AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036278

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036278 MtrunA17_Chr7g0224271 98.077 676 11 1 1 676 1 674 0.0 1377
MsaG036278 MtrunA17_Chr4g0057891 41.604 399 211 6 290 676 133 521 2.01e-96 306
MsaG036278 MtrunA17_Chr8g0376381 41.895 401 191 10 286 675 153 522 1.76e-94 302
MsaG036278 MtrunA17_Chr2g0306641 42.250 400 190 10 292 676 115 488 3.88e-93 297
MsaG036278 MtrunA17_Chr3g0110971 40.146 411 199 9 286 675 203 587 7.28e-93 300
MsaG036278 MtrunA17_Chr7g0245601 41.910 377 186 6 306 675 442 792 1.58e-92 304
MsaG036278 MtrunA17_Chr8g0357601 40.863 394 206 6 294 675 174 552 4.15e-88 286
MsaG036278 MtrunA17_Chr5g0447691 40.204 393 205 5 295 675 170 544 5.39e-88 285
MsaG036278 MtrunA17_Chr4g0057291 38.303 389 217 7 296 674 286 661 5.94e-82 273
MsaG036278 MtrunA17_Chr1g0159461 39.589 389 201 6 301 675 224 592 5.94e-81 268
MsaG036278 MtrunA17_Chr2g0319091 36.721 433 238 5 260 674 164 578 1.16e-79 264
MsaG036278 MtrunA17_Chr4g0038761 39.516 372 206 8 306 675 88 442 2.66e-78 257
MsaG036278 MtrunA17_Chr3g0101141 32.554 556 297 10 164 675 21 542 2.02e-77 257
MsaG036278 MtrunA17_Chr5g0445331 33.551 459 269 7 235 675 92 532 3.34e-77 256
MsaG036278 MtrunA17_Chr3g0125811 37.887 388 209 5 301 675 202 570 3.12e-76 255
MsaG036278 MtrunA17_Chr4g0076091 35.878 393 221 5 297 676 170 544 4.61e-71 240
MsaG036278 MtrunA17_Chr2g0290241 33.915 401 218 8 297 676 52 426 9.83e-70 233
MsaG036278 MtrunA17_Chr1g0183641 35.128 390 224 10 292 676 113 478 3.59e-65 223
MsaG036278 MtrunA17_Chr4g0031721 34.000 400 234 6 288 674 301 683 4.28e-65 228
MsaG036278 MtrunA17_Chr5g0424131 36.930 417 210 17 290 676 107 500 2.03e-64 221
MsaG036278 MtrunA17_Chr1g0200401 34.140 413 220 14 292 675 70 459 5.96e-64 219
MsaG036278 MtrunA17_Chr3g0114841 37.346 407 218 14 292 676 110 501 6.63e-64 220
MsaG036278 MtrunA17_Chr3g0085681 30.140 428 227 5 303 675 26 436 7.60e-62 213
MsaG036278 MtrunA17_Chr7g0238891 32.398 392 234 5 297 675 367 740 4.58e-59 212
MsaG036278 MtrunA17_Chr4g0031701 31.910 398 234 5 293 672 245 623 1.04e-58 209
MsaG036278 MtrunA17_Chr4g0031731 31.910 398 234 5 293 672 245 623 1.55e-57 206
MsaG036278 MtrunA17_Chr2g0328391 31.807 393 237 6 296 674 293 668 1.02e-56 204
MsaG036278 MtrunA17_Chr4g0031741 31.714 391 234 7 297 674 279 649 1.60e-56 203
MsaG036278 MtrunA17_Chr2g0328341 30.848 389 240 6 297 674 267 637 2.18e-56 202
MsaG036278 MtrunA17_Chr4g0031711 31.671 401 237 7 292 674 351 732 2.18e-55 201
MsaG036278 MtrunA17_Chr2g0328381 30.334 389 242 6 297 674 270 640 9.84e-55 198
MsaG036278 MtrunA17_Chr2g0328411 29.771 393 242 5 295 674 282 653 4.03e-54 197
MsaG036278 MtrunA17_Chr2g0328431 30.227 397 235 8 295 674 282 653 1.83e-51 189
MsaG036278 MtrunA17_Chr4g0039661 33.962 371 208 8 318 675 209 555 1.20e-50 185
MsaG036278 MtrunA17_Chr2g0294461 31.514 403 248 9 288 675 196 585 2.84e-49 181
MsaG036278 MtrunA17_Chr3g0085361 30.747 348 181 5 383 675 8 350 2.37e-48 173
MsaG036278 MtrunA17_Chr5g0401131 30.923 401 240 11 295 675 107 490 7.79e-48 176
MsaG036278 MtrunA17_Chr2g0294471 29.703 404 251 11 288 675 190 576 1.60e-46 174
MsaG036278 MtrunA17_Chr2g0328401 27.933 358 226 5 297 642 285 622 6.22e-45 170
MsaG036278 MtrunA17_Chr2g0328361 28.093 388 227 7 297 674 269 614 1.86e-44 168
MsaG036278 MtrunA17_Chr7g0270031 29.723 397 253 9 288 675 175 554 3.26e-44 167
MsaG036278 MtrunA17_Chr8g0383981 32.426 404 221 15 289 674 126 495 5.49e-42 159
MsaG036278 MtrunA17_Chr1g0194621 28.502 414 273 12 275 675 115 518 3.68e-41 157
MsaG036278 MtrunA17_Chr2g0328371 34.286 245 150 4 389 622 1 245 3.87e-41 150
MsaG036278 MtrunA17_Chr5g0396491 36.797 231 124 2 291 518 45 256 8.08e-40 152
MsaG036278 MtrunA17_Chr5g0396491 43.624 149 84 0 527 675 330 478 5.45e-39 150
MsaG036278 MtrunA17_Chr4g0038851 44.595 148 82 0 528 675 322 469 1.92e-39 151
MsaG036278 MtrunA17_Chr4g0038851 35.526 228 131 2 291 518 37 248 3.06e-35 139
MsaG036278 MtrunA17_Chr7g0236851 34.007 297 175 8 394 674 365 656 1.58e-38 151
MsaG036278 MtrunA17_Chr7g0230771 29.124 388 238 11 299 676 372 732 8.41e-36 144
MsaG036278 MtrunA17_Chr7g0230731 29.124 388 238 11 299 676 369 729 4.48e-35 142
MsaG036278 MtrunA17_Chr6g0468561 29.383 405 216 13 303 675 296 662 4.98e-34 138
MsaG036278 MtrunA17_Chr4g0053901 25.714 420 254 9 302 674 59 467 1.46e-32 131
MsaG036278 MtrunA17_Chr2g0329591 26.076 395 254 10 302 675 74 451 2.59e-32 130
MsaG036278 MtrunA17_Chr4g0012071 28.238 386 235 12 303 676 197 552 6.47e-32 130
MsaG036278 MtrunA17_Chr5g0401421 25.298 419 259 11 302 674 35 445 5.33e-31 126
MsaG036278 MtrunA17_Chr8g0373561 27.226 393 242 14 299 676 179 542 6.35e-29 121
MsaG036278 MtrunA17_Chr8g0344101 23.587 407 251 12 301 675 222 600 1.66e-19 92.8
MsaG036278 MtrunA17_Chr2g0323111 26.286 175 120 3 507 674 133 305 4.69e-14 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036278 AT1G66350.1 44.103 390 184 11 293 676 146 507 1.29e-97 310
MsaG036278 AT3G03450.1 42.347 392 197 8 293 675 174 545 1.09e-95 306
MsaG036278 AT3G54220.1 41.709 398 196 6 287 674 278 649 1.11e-92 301
MsaG036278 AT1G14920.1 40.712 393 197 9 293 675 163 529 4.25e-90 291
MsaG036278 AT5G17490.1 42.967 391 190 10 293 675 151 516 2.51e-89 288
MsaG036278 AT2G01570.1 40.458 393 198 10 293 675 215 581 4.59e-87 284
MsaG036278 AT5G41920.1 40.759 395 202 9 289 675 30 400 2.48e-86 277
MsaG036278 AT2G04890.1 39.949 393 209 5 289 675 42 413 8.47e-84 270
MsaG036278 AT5G48150.1 39.481 385 204 5 303 675 123 490 7.20e-83 270
MsaG036278 AT5G48150.4 39.481 385 204 5 303 675 123 490 7.20e-83 270
MsaG036278 AT5G48150.2 39.481 385 204 5 303 675 123 490 7.20e-83 270
MsaG036278 AT5G48150.3 39.481 385 204 5 303 675 123 490 7.20e-83 270
MsaG036278 AT1G50600.3 38.560 389 209 6 299 675 156 526 2.03e-73 246
MsaG036278 AT1G50600.1 38.560 389 209 6 299 675 227 597 6.36e-73 247
MsaG036278 AT1G50600.2 38.560 389 209 6 299 675 231 601 7.54e-73 247
MsaG036278 AT1G21450.1 36.434 387 217 4 301 675 224 593 2.94e-72 245
MsaG036278 AT1G55580.1 37.438 406 226 8 298 675 40 445 1.22e-71 239
MsaG036278 AT4G17230.1 36.061 391 220 4 297 675 153 525 8.02e-68 231
MsaG036278 AT4G17230.2 36.061 391 220 4 297 675 153 525 8.02e-68 231
MsaG036278 AT1G63100.1 34.596 396 227 7 297 674 247 628 4.36e-67 232
MsaG036278 AT1G63100.2 34.596 396 227 7 297 674 272 653 7.38e-67 232
MsaG036278 AT1G07520.2 32.010 403 244 8 288 674 140 528 8.11e-59 207
MsaG036278 AT1G07520.4 32.010 403 244 8 288 674 140 528 8.11e-59 207
MsaG036278 AT1G07520.3 31.841 402 246 7 288 674 305 693 3.22e-58 209
MsaG036278 AT1G07520.1 31.841 402 246 7 288 674 304 692 3.54e-58 209
MsaG036278 AT5G59450.1 29.442 394 246 6 297 675 222 598 3.69e-57 204
MsaG036278 AT3G46600.1 30.326 399 246 5 290 675 200 579 4.64e-57 204
MsaG036278 AT3G46600.3 30.326 399 246 5 290 675 168 547 8.07e-57 202
MsaG036278 AT3G46600.2 30.670 388 237 5 301 675 3 371 3.92e-56 196
MsaG036278 AT1G07530.1 31.538 390 235 6 299 674 393 764 3.35e-55 202
MsaG036278 AT2G29065.1 29.820 389 244 7 302 674 252 627 9.47e-55 198
MsaG036278 AT5G66770.1 35.294 391 216 10 299 675 217 584 1.78e-53 194
MsaG036278 AT2G29060.2 30.612 392 238 7 299 674 281 654 8.33e-53 193
MsaG036278 AT2G29060.1 30.612 392 238 7 299 674 318 691 1.23e-52 193
MsaG036278 AT2G37650.2 29.923 391 243 5 297 675 330 701 1.39e-52 193
MsaG036278 AT2G37650.1 29.923 391 243 5 297 675 342 713 1.84e-52 193
MsaG036278 AT4G08250.1 32.432 407 228 14 290 674 95 476 5.48e-49 179
MsaG036278 AT3G50650.1 33.962 371 205 9 322 675 195 542 6.36e-46 172
MsaG036278 AT4G37650.1 29.397 398 243 10 302 675 146 529 3.14e-43 164
MsaG036278 AT5G52510.1 30.250 400 224 9 303 675 269 640 6.38e-40 156
MsaG036278 AT1G50420.1 45.333 150 82 0 527 676 331 480 7.28e-39 150
MsaG036278 AT1G50420.1 38.496 226 125 2 293 518 48 259 7.57e-38 147
MsaG036278 AT3G49950.1 29.426 401 234 13 301 674 29 407 9.37e-33 131
MsaG036278 AT2G45160.1 27.829 327 204 9 369 676 327 640 1.89e-28 121
MsaG036278 AT4G00150.1 27.431 401 238 13 284 676 200 555 2.74e-28 120
MsaG036278 AT4G36710.1 26.884 398 256 11 289 675 112 485 5.29e-28 118
MsaG036278 AT3G60630.1 28.852 305 191 10 390 676 327 623 8.91e-28 119
MsaG036278 AT3G13840.1 24.557 395 245 14 302 675 148 510 1.93e-14 77.0

Find 169 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCAATATCAACAATTT+TGG 0.134391 7:-30786763 MsaT036278.1:CDS
TGTTGCATATATTGATTATT+TGG 0.203768 7:+30786894 None:intergenic
GTAGAAGCTTGTGGTGAATT+AGG 0.209436 7:+30787074 None:intergenic
CTATCCAAAATTGTTGATAT+TGG 0.238972 7:+30786759 None:intergenic
AATAGTAATAATCAACAAAA+AGG 0.242712 7:-30786954 MsaT036278.1:CDS
TGCTTCAAACGCCTCGAAAA+TGG 0.246534 7:+30785672 None:intergenic
GACTCAATCAAGGATATTAT+TGG 0.253341 7:-30785005 MsaT036278.1:CDS
TGTATTCATGTGATGGTTAC+AGG 0.259318 7:-30784979 MsaT036278.1:CDS
GGCATGAGAGGTTGGAGAAA+TGG 0.280692 7:-30785084 MsaT036278.1:CDS
GATGGTAAGTTTAGGCTTTG+TGG 0.292426 7:+30787227 None:intergenic
GTTAGGGTTAATGTTTCTAA+TGG 0.293041 7:+30787097 None:intergenic
TAGAAGCTTGTGGTGAATTA+GGG 0.297220 7:+30787075 None:intergenic
GGCAAGCCTGGTAGACAATT+TGG 0.300466 7:+30785731 None:intergenic
CTTGATTGAGTCACTGCATT+AGG 0.311878 7:+30785016 None:intergenic
GTATCATCAAATGGGATCTA+TGG 0.314373 7:-30786631 MsaT036278.1:CDS
TCATGAAATCATTATCTAAA+TGG 0.317694 7:+30787129 None:intergenic
TCTCTCAAATTTGACTTAGA+TGG 0.323118 7:-30787206 MsaT036278.1:CDS
AACCGTCTCCACCGTGTTCC+TGG 0.336032 7:-30785367 MsaT036278.1:CDS
ATTTGTTTGATTTGAAGAAT+TGG 0.347254 7:+30787313 None:intergenic
CATCGACCTTGATATCCTTC+AGG 0.351187 7:-30785632 MsaT036278.1:CDS
ACTCAATCAAGGATATTATT+GGG 0.352700 7:-30785004 MsaT036278.1:CDS
TTGACGATGACTTGGCTGTT+AGG 0.362582 7:+30785848 None:intergenic
TCTAAGAACTCTTCAATTGC+TGG 0.367894 7:+30786852 None:intergenic
CTAACAGTTCCTATTCCAAT+TGG 0.374016 7:-30786528 MsaT036278.1:CDS
CCGCCGTGTAGGCGAGGCTT+TGG 0.375077 7:-30785404 MsaT036278.1:CDS
GCTTGTGGTGAATTAGGGTT+AGG 0.379242 7:+30787080 None:intergenic
ATAAAGGTTGTTTGTTGCTT+GGG 0.381702 7:-30784946 MsaT036278.1:CDS
GTCACTGCATTAGGACTTAG+AGG 0.385725 7:+30785025 None:intergenic
TGGTGCTTGGTCTCTTATCA+TGG 0.387315 7:+30785321 None:intergenic
GATAAAGGTTGTTTGTTGCT+TGG 0.388205 7:-30784947 MsaT036278.1:CDS
GATAGAGAGGCATGAGAGGT+TGG 0.388288 7:-30785092 MsaT036278.1:CDS
GCTTCCCACATTGAACTATC+TGG 0.392042 7:+30787164 None:intergenic
AGGACTTCCATAGGGTTTGA+TGG 0.398057 7:+30785763 None:intergenic
TCCTCAAGTTGTTCTCCAAC+GGG 0.400097 7:+30785448 None:intergenic
AATGCAAAGCCTCGAGAAAT+CGG 0.401833 7:+30785242 None:intergenic
GCCAATGCCTGCATGAATGC+AGG 0.402364 7:+30785598 None:intergenic
ATGCAAATTCAAAGCCTATC+AGG 0.406320 7:-30787017 MsaT036278.1:CDS
TACAGGTTAACTGAGGATAA+AGG 0.412392 7:-30784962 MsaT036278.1:CDS
CACAAGCTTTACAACAAGAA+AGG 0.417444 7:-30786593 MsaT036278.1:CDS
ATTTGAGAGATGGTAAGTTT+AGG 0.417615 7:+30787219 None:intergenic
TTTGTTTGATTTGAAGAATT+GGG 0.419908 7:+30787314 None:intergenic
TTAGGGTTAATGTTTCTAAT+GGG 0.423047 7:+30787098 None:intergenic
CAGGCATTGGCTGCACGGCC+CGG 0.426882 7:-30785586 MsaT036278.1:CDS
GAGTGATATGTATCATCAAA+TGG 0.430639 7:-30786640 MsaT036278.1:CDS
TCCACAAGTGTCACTATGTT+TGG 0.431028 7:+30785301 None:intergenic
ATATATTTGCACCGGAGATT+CGG 0.433485 7:-30785144 MsaT036278.1:CDS
CTCGAAAATGGCTTGGTTAG+CGG 0.435294 7:+30785684 None:intergenic
GGCCGTGCATCGAATCAGTT+AGG 0.436845 7:-30785531 MsaT036278.1:CDS
TCATGCAGGCATTGGCTGCA+CGG 0.438295 7:-30785591 MsaT036278.1:CDS
CCGGAGGAAAGGAGCACCAC+CGG 0.441381 7:+30785567 None:intergenic
GGAGAAATGGAGGAAGATAA+TGG 0.442415 7:-30785071 MsaT036278.1:CDS
GGAAACCGGACGATGCTTAA+CGG 0.443248 7:-30785509 MsaT036278.1:CDS
AAAATGATGAATTCACTTTG+TGG 0.443783 7:-30787371 MsaT036278.1:CDS
ATTACCTGCTCAATTCCAAT+TGG 0.449064 7:+30786513 None:intergenic
GTGTTCCTGGCAATCATCTC+GGG 0.453444 7:-30785354 MsaT036278.1:CDS
GCTACGATGTTCCGAATCTC+CGG 0.453601 7:+30785133 None:intergenic
ATCGAATCAGTTAGGGAAAC+CGG 0.454197 7:-30785523 MsaT036278.1:CDS
CAAAATTCTTCAAGCTATTG+TGG 0.454394 7:-30786726 MsaT036278.1:CDS
TAAATCACTACAAGAATTTG+TGG 0.454463 7:+30787271 None:intergenic
TGCTCAATTCCAATTGGAAT+AGG 0.456239 7:+30786519 None:intergenic
GGTCTCTTATCATGGACAAC+AGG 0.459964 7:+30785329 None:intergenic
AGCAAGAAGCAAGCCATAAC+GGG 0.462121 7:-30785279 MsaT036278.1:CDS
GCCCTTGGTGCTGATTCCAC+TGG 0.463860 7:+30785181 None:intergenic
CCGTGTAGGCGAGGCTTTGG+CGG 0.466492 7:-30785401 MsaT036278.1:CDS
CGTGTTCCTGGCAATCATCT+CGG 0.467483 7:-30785355 MsaT036278.1:CDS
ACATTGAACTATCTGGTGAT+TGG 0.469373 7:+30787171 None:intergenic
TACTGCTCCACCTTAGCCCT+TGG 0.470876 7:+30785166 None:intergenic
TGACGATGACTTGGCTGTTA+GGG 0.471157 7:+30785849 None:intergenic
CTTGTGGTGAATTAGGGTTA+GGG 0.477041 7:+30787081 None:intergenic
ACGGCCCTACGCCGGTGATC+CGG 0.477412 7:+30785548 None:intergenic
TGTGCTGAAGCAGTGGCAAA+AGG 0.478803 7:-30785994 MsaT036278.1:CDS
AACACGGTGGAGACGGTTAA+CGG 0.479130 7:+30785372 None:intergenic
ATGAGAGGTTGGAGAAATGG+AGG 0.480103 7:-30785081 MsaT036278.1:CDS
CACTGGAAAAGTGGCGTCCA+AGG 0.485225 7:+30785198 None:intergenic
TATTCCAATTGGAATTGAGC+AGG 0.485481 7:-30786517 MsaT036278.1:intron
TTCCATAGGGTTTGATGGGA+AGG 0.488537 7:+30785768 None:intergenic
GCATTGGCTGCACGGCCCGG+TGG 0.490051 7:-30785583 MsaT036278.1:CDS
ATCGACCTTGATATCCTTCA+GGG 0.491571 7:-30785631 MsaT036278.1:CDS
GCCTGCATTCATGCAGGCAT+TGG 0.492416 7:-30785599 MsaT036278.1:CDS
GTGTCACTATGTTTGGTGCT+TGG 0.501545 7:+30785308 None:intergenic
CCGCCAAAGCCTCGCCTACA+CGG 0.501971 7:+30785401 None:intergenic
GTGCTGAAGCAGTGGCAAAA+GGG 0.503557 7:-30785993 MsaT036278.1:CDS
AGTGATATGTATCATCAAAT+GGG 0.505152 7:-30786639 MsaT036278.1:CDS
TACCTTCCCATCAAACCCTA+TGG 0.506323 7:-30785770 MsaT036278.1:CDS
TCCCTAACTGATTCGATGCA+CGG 0.507816 7:+30785529 None:intergenic
AGAGGATAGAGAGGCATGAG+AGG 0.511494 7:-30785096 MsaT036278.1:CDS
CCGGTGGTGCTCCTTTCCTC+CGG 0.515736 7:-30785567 MsaT036278.1:CDS
CAAACGCCTCGAAAATGGCT+TGG 0.518445 7:+30785677 None:intergenic
AAGGTGAGGAGAGGATAGAG+AGG 0.521365 7:-30785105 MsaT036278.1:CDS
GGAAGATAATGGAAGGGAAA+GGG 0.523029 7:-30785060 MsaT036278.1:CDS
TAACTCCGTTAAGCATCGTC+CGG 0.530160 7:+30785504 None:intergenic
TTCCTCCGGATCACCGGCGT+AGG 0.532300 7:-30785553 MsaT036278.1:CDS
CCAATGGCCTGCATTCATGC+AGG 0.532300 7:-30785605 MsaT036278.1:CDS
GCTCCTTTCCTCCGGATCAC+CGG 0.533425 7:-30785559 MsaT036278.1:CDS
TAGATCTTGAGGACTTCCAT+AGG 0.534706 7:+30785754 None:intergenic
CCCTTCGAGTTCCACCCCGT+TGG 0.536028 7:-30785463 MsaT036278.1:CDS
CCAACGGGGTGGAACTCGAA+GGG 0.537973 7:+30785463 None:intergenic
CAGGCCATTGGTAGCCCTGA+AGG 0.538080 7:+30785617 None:intergenic
TGCCAGGAACACGGTGGAGA+CGG 0.538538 7:+30785365 None:intergenic
AGAATCTCTAAGTGCAAGAC+TGG 0.538771 7:-30785878 MsaT036278.1:CDS
GGACTTCCATAGGGTTTGAT+GGG 0.540263 7:+30785764 None:intergenic
GGAATCAGCACCAAGGGCTA+AGG 0.543556 7:-30785176 MsaT036278.1:CDS
ATCCTCAAGTTGTTCTCCAA+CGG 0.543558 7:+30785447 None:intergenic
CTAATGCAGTGACTCAATCA+AGG 0.544135 7:-30785015 MsaT036278.1:CDS
TTGGGTTTGTATTCATGTGA+TGG 0.544918 7:-30784986 MsaT036278.1:CDS
AGACAATTTGGTAGATCTTG+AGG 0.549770 7:+30785743 None:intergenic
AAGGTTGTTTGTTGCTTGGG+TGG 0.550029 7:-30784943 MsaT036278.1:CDS
TCCAGTGGAATCAGCACCAA+GGG 0.550198 7:-30785182 MsaT036278.1:CDS
AGATCTTGAGGACTTCCATA+GGG 0.551151 7:+30785755 None:intergenic
AGGTTCCCGAGATGATTGCC+AGG 0.551741 7:+30785349 None:intergenic
GAGCAAGAAGCAAGCCATAA+CGG 0.555779 7:-30785280 MsaT036278.1:CDS
TCTAAGTCAAATTTGAGAGA+TGG 0.557349 7:+30787209 None:intergenic
TGTAACTATAACTATGCTCA+AGG 0.559054 7:-30787041 MsaT036278.1:CDS
ATCAGCACCAAGGGCTAAGG+TGG 0.559830 7:-30785173 MsaT036278.1:CDS
TGCTGAAGCAGTGGCAAAAG+GGG 0.561256 7:-30785992 MsaT036278.1:CDS
ATGGAAGGGAAAGGGTTCAA+AGG 0.562746 7:-30785052 MsaT036278.1:CDS
GGTAGCCCTGAAGGATATCA+AGG 0.563155 7:+30785626 None:intergenic
GGATATCAAGGTCGATGACA+TGG 0.564199 7:+30785638 None:intergenic
GATCTACCAAATTGTCTACC+AGG 0.564411 7:-30785737 MsaT036278.1:CDS
CGGAGGAAAGGAGCACCACC+GGG 0.564457 7:+30785568 None:intergenic
GGTAGATAAACATGATGAGG+AGG 0.565470 7:+30785789 None:intergenic
GAAGGTAGATAAACATGATG+AGG 0.572092 7:+30785786 None:intergenic
AAATGGAGGAAGATAATGGA+AGG 0.574690 7:-30785067 MsaT036278.1:CDS
GAGGTGGTGAACAACCTGAT+AGG 0.574741 7:+30787003 None:intergenic
ACGCCGGTGATCCGGAGGAA+AGG 0.576975 7:+30785556 None:intergenic
CTATTATCGCTGCTTCTGCA+TGG 0.579656 7:-30784910 MsaT036278.1:CDS
ACATGAAGCTACTCGTTGCA+TGG 0.580886 7:+30785906 None:intergenic
TCCAACGGGGTGGAACTCGA+AGG 0.584775 7:+30785462 None:intergenic
TCCTCCGGATCACCGGCGTA+GGG 0.584832 7:-30785552 MsaT036278.1:CDS
CCTGCATGAATGCAGGCCAT+TGG 0.587372 7:+30785605 None:intergenic
GTTTAACCGCCGTGTAGGCG+AGG 0.587763 7:-30785410 MsaT036278.1:CDS
GTGTTACTACTCTATTGAGC+TGG 0.588376 7:+30785941 None:intergenic
GGGAATACATGCTAGCAAGA+AGG 0.591113 7:-30785972 MsaT036278.1:CDS
CTACTCTATTGAGCTGGTGT+AGG 0.595572 7:+30785947 None:intergenic
TGCTGATTCCACTGGAAAAG+TGG 0.596664 7:+30785189 None:intergenic
CGGAACATCGTAGCATGTGA+AGG 0.597195 7:-30785124 MsaT036278.1:CDS
GAATTAAATACTCTATGAAG+TGG 0.597232 7:+30786918 None:intergenic
TTCGATGCACGGCCCTACGC+CGG 0.597944 7:+30785540 None:intergenic
GCCGTGCATCGAATCAGTTA+GGG 0.599094 7:-30785530 MsaT036278.1:CDS
CAACGGGGTGGAACTCGAAG+GGG 0.601149 7:+30785464 None:intergenic
TCTAGCATGTGCTGAAGCAG+TGG 0.602572 7:-30786001 MsaT036278.1:CDS
CGAGATGATTGCCAGGAACA+CGG 0.603757 7:+30785356 None:intergenic
ATCACCAGATAGTTCAATGT+GGG 0.605621 7:-30787168 MsaT036278.1:CDS
ATATCCTTCAGGGCTACCAA+TGG 0.606276 7:-30785621 MsaT036278.1:CDS
GCCCTACGCCGGTGATCCGG+AGG 0.610803 7:+30785551 None:intergenic
AGGAAGATAATGGAAGGGAA+AGG 0.613642 7:-30785061 MsaT036278.1:CDS
CCCCGTTGGAGAACAACTTG+AGG 0.617458 7:-30785449 MsaT036278.1:CDS
CATCGTAGCATGTGAAGGTG+AGG 0.619286 7:-30785119 MsaT036278.1:CDS
GGAGCAGTATATATTTGCAC+CGG 0.624565 7:-30785152 MsaT036278.1:CDS
TTCCAGTGGAATCAGCACCA+AGG 0.632372 7:-30785183 MsaT036278.1:CDS
AATGGAGGAAGATAATGGAA+GGG 0.644464 7:-30785066 MsaT036278.1:CDS
ATTTGACTTAGATGGTGATG+TGG 0.646549 7:-30787198 MsaT036278.1:CDS
CGTTGCATGGAGTCACCTAG+TGG 0.646828 7:+30785919 None:intergenic
AATAGAGTAGTAACACCACT+AGG 0.648126 7:-30785934 MsaT036278.1:CDS
CACATGTTTAACCGCCGTGT+AGG 0.660169 7:-30785415 MsaT036278.1:CDS
CAAGTTGTTCTCCAACGGGG+TGG 0.668903 7:+30785452 None:intergenic
ACACGGCGGTTAAACATGTG+CGG 0.674730 7:+30785418 None:intergenic
TAGCATGTGAAGGTGAGGAG+AGG 0.683443 7:-30785114 MsaT036278.1:CDS
ACCAAACATAGTGACACTTG+TGG 0.687522 7:-30785302 MsaT036278.1:CDS
GATGACGATGATAACGATGA+TGG 0.694572 7:+30785811 None:intergenic
TATATGTAGGAACAAGACAG+TGG 0.702151 7:-30786045 MsaT036278.1:intron
TTATTGTAAGTAGAAGCTTG+TGG 0.706364 7:+30787065 None:intergenic
CCAATTTGTGATGCAAAACG+AGG 0.711917 7:+30786984 None:intergenic
AATCACCAGATAGTTCAATG+TGG 0.716750 7:-30787169 MsaT036278.1:CDS
TGATGGTTACAGGTTAACTG+AGG 0.719493 7:-30784969 MsaT036278.1:CDS
CCTCAAGTTGTTCTCCAACG+GGG 0.734185 7:+30785449 None:intergenic
ATTTGTGATGCAAAACGAGG+TGG 0.735195 7:+30786987 None:intergenic
GATGATTGCCAGGAACACGG+TGG 0.742020 7:+30785359 None:intergenic
CCAAAGCCTCGCCTACACGG+CGG 0.759718 7:+30785404 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 30784901 30787390 30784901 ID=MsaG036278
Chr7 mRNA 30784901 30787390 30784901 ID=MsaT036278.1;Parent=MsaG036278
Chr7 exon 30784901 30786058 30784901 ID=MsaT036278.1.exon2;Parent=MsaT036278.1
Chr7 CDS 30784901 30786058 30784901 ID=cds.MsaT036278.1;Parent=MsaT036278.1
Chr7 exon 30786518 30787390 30786518 ID=MsaT036278.1.exon1;Parent=MsaT036278.1
Chr7 CDS 30786518 30787390 30786518 ID=cds.MsaT036278.1;Parent=MsaT036278.1
Gene Sequence

>MsaT036278.1

ATGATGAATTCACTTTGTGGAAGCTCAAATTCTCTCAAAGAAAAATGTCTTCAACCCAATTCTTCAAATCAAACAAATACTCACTCAAAGAAAAATGCCACAAATTCTTGTAGTGATTTAGAGCAAATTAATGTTCTAACTCCACAAAGCCTAAACTTACCATCTCTCAAATTTGACTTAGATGGTGATGTGGAAGTCCAATCACCAGATAGTTCAATGTGGGAAGCATTTTTCAATGACCATTTAGATAATGATTTCATGATATCATCACCCATTAGAAACATTAACCCTAACCCTAATTCACCACAAGCTTCTACTTACAATAATTGTAACTATAACTATGCTCAAGGCATGCAAATTCAAAGCCTATCAGGTTGTTCACCACCTCGTTTTGCATCACAAATTGGTTCATTAAATAGTAATAATCAACAAAAAGGGAAAGGACTTAGTCCACTTCATAGAGTATTTAATTCACCAAATAATCAATATATGCAACATGTTGAAAATCTTTCATTGCCAGCAATTGAAGAGTTCTTAGAAGATTTTCAAGGTGATGTTGATCACTTTTCATCAACAAAGGTTTCTTCTGAATGTTTTGACATGGAAACTCCAATATCAACAATTTTGGATAGTTTAACAATGCAAAATTCTTCAAGCTATTGTGGTTCAGTTAATGAAGAATCTACTATTTTGCATGGTGGTAATTCTTCTTCTCAACTTTCTCAAGAGAGTGATATGTATCATCAAATGGGATCTATGGCTAGTGCTTCTCTTTCACAAGCTTTACAACAAGAAAGGTACCAAGAGAAACATCAAAAGATGCAAGCTCAACAGCAGAATCTAACAGTTCCTATTCCAATTGGAATTGAGCAGGAACAAGACAGTGGACTTCAACTAGTGCACTTACTTCTAGCATGTGCTGAAGCAGTGGCAAAAGGGGAATACATGCTAGCAAGAAGGTACCTACACCAGCTCAATAGAGTAGTAACACCACTAGGTGACTCCATGCAACGAGTAGCTTCATGTTTCACAGAATCTCTAAGTGCAAGACTGGCTGCAACCCTAACAGCCAAGTCATCGTCAACCAAAAAGTTAACACCATCATCGTTATCATCGTCATCCTCCTCATCATGTTTATCTACCTTCCCATCAAACCCTATGGAAGTCCTCAAGATCTACCAAATTGTCTACCAGGCTTGCCCTTACATAAAATTTGCACACTTTACCGCTAACCAAGCCATTTTCGAGGCGTTTGAAGCAGAAGAGCGTGTCCATGTCATCGACCTTGATATCCTTCAGGGCTACCAATGGCCTGCATTCATGCAGGCATTGGCTGCACGGCCCGGTGGTGCTCCTTTCCTCCGGATCACCGGCGTAGGGCCGTGCATCGAATCAGTTAGGGAAACCGGACGATGCTTAACGGAGTTAGCTCATTCTCTTCGTATCCCCTTCGAGTTCCACCCCGTTGGAGAACAACTTGAGGATCTTAAACCGCACATGTTTAACCGCCGTGTAGGCGAGGCTTTGGCGGTTAACACCGTTAACCGTCTCCACCGTGTTCCTGGCAATCATCTCGGGAACCTGTTGTCCATGATAAGAGACCAAGCACCAAACATAGTGACACTTGTGGAGCAAGAAGCAAGCCATAACGGGCCGTACTTTTTGGGCCGATTTCTCGAGGCTTTGCATTACTACTCTGCAATTTTCGACTCCTTGGACGCCACTTTTCCAGTGGAATCAGCACCAAGGGCTAAGGTGGAGCAGTATATATTTGCACCGGAGATTCGGAACATCGTAGCATGTGAAGGTGAGGAGAGGATAGAGAGGCATGAGAGGTTGGAGAAATGGAGGAAGATAATGGAAGGGAAAGGGTTCAAAGGAGTGCCTCTAAGTCCTAATGCAGTGACTCAATCAAGGATATTATTGGGTTTGTATTCATGTGATGGTTACAGGTTAACTGAGGATAAAGGTTGTTTGTTGCTTGGGTGGCAAGACAGAGCTATTATCGCTGCTTCTGCATGGCGATGCTGA
Protein sequence

>MsaT036278.1

MMNSLCGSSNSLKEKCLQPNSSNQTNTHSKKNATNSCSDLEQINVLTPQSLNLPSLKFDLDGDVEVQSPDSSMWEAFFNDHLDNDFMISSPIRNINPNPNSPQASTYNNCNYNYAQGMQIQSLSGCSPPRFASQIGSLNSNNQQKGKGLSPLHRVFNSPNNQYMQHVENLSLPAIEEFLEDFQGDVDHFSSTKVSSECFDMETPISTILDSLTMQNSSSYCGSVNEESTILHGGNSSSQLSQESDMYHQMGSMASASLSQALQQERYQEKHQKMQAQQQNLTVPIPIGIEQEQDSGLQLVHLLLACAEAVAKGEYMLARRYLHQLNRVVTPLGDSMQRVASCFTESLSARLAATLTAKSSSTKKLTPSSLSSSSSSSCLSTFPSNPMEVLKIYQIVYQACPYIKFAHFTANQAIFEAFEAEERVHVIDLDILQGYQWPAFMQALAARPGGAPFLRITGVGPCIESVRETGRCLTELAHSLRIPFEFHPVGEQLEDLKPHMFNRRVGEALAVNTVNRLHRVPGNHLGNLLSMIRDQAPNIVTLVEQEASHNGPYFLGRFLEALHYYSAIFDSLDATFPVESAPRAKVEQYIFAPEIRNIVACEGEERIERHERLEKWRKIMEGKGFKGVPLSPNAVTQSRILLGLYSCDGYRLTEDKGCLLLGWQDRAIIAASAWRC*