AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036295


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036295 MtrunA17_Chr7g0224581 96.396 222 5 1 1 222 66 284 1.59e-154 429
MsaG036295 MtrunA17_Chr5g0416581 57.292 96 24 3 1 79 83 178 2.23e-25 102
MsaG036295 MtrunA17_Chr2g0277361 75.000 64 10 1 1 58 146 209 1.18e-24 100
MsaG036295 MtrunA17_Chr8g0350941 70.968 62 18 0 1 62 96 157 1.75e-24 99.0
MsaG036295 MtrunA17_Chr4g0060601 89.362 47 5 0 1 47 113 159 6.32e-24 98.6
MsaG036295 MtrunA17_Chr1g0204001 70.968 62 18 0 1 62 80 141 7.89e-24 97.1
MsaG036295 MtrunA17_Chr1g0180911 85.106 47 7 0 1 47 65 111 1.18e-23 94.0
MsaG036295 MtrunA17_Chr1g0164881 62.162 74 28 0 1 74 48 121 2.85e-23 93.6
MsaG036295 MtrunA17_Chr1g0211941 83.333 48 8 0 1 48 49 96 1.79e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036295 AT3G27010.2 56.061 198 64 7 1 192 94 274 2.69e-49 164
MsaG036295 AT3G27010.1 56.061 198 64 7 1 192 94 274 2.69e-49 164
MsaG036295 AT1G58100.1 75.000 64 10 1 1 58 76 139 2.63e-25 102
MsaG036295 AT5G41030.1 35.437 206 69 11 1 204 84 227 1.06e-24 98.2
MsaG036295 AT2G45680.1 64.706 68 24 0 1 68 91 158 5.09e-24 98.2
MsaG036295 AT3G47620.1 91.489 47 4 0 1 47 133 179 6.34e-24 99.4
MsaG036295 AT1G69690.1 85.417 48 7 0 1 48 68 115 6.60e-24 97.4
MsaG036295 AT1G35560.1 73.438 64 11 1 1 58 71 134 7.78e-24 97.4
MsaG036295 AT1G72010.1 87.234 47 6 0 1 47 79 125 1.98e-23 97.1
MsaG036295 AT5G51910.1 65.625 64 22 0 1 64 73 136 2.30e-23 95.5
MsaG036295 AT5G51910.2 65.625 64 22 0 1 64 73 136 2.30e-23 95.5
MsaG036295 AT5G08330.1 72.881 59 16 0 1 59 47 105 2.63e-23 94.4
MsaG036295 AT5G23280.1 83.333 48 8 0 1 48 55 102 1.40e-22 92.4
MsaG036295 AT2G37000.1 84.091 44 7 0 1 44 59 102 4.32e-19 82.0
MsaG036295 AT3G45150.1 58.140 43 18 0 1 43 33 75 1.10e-11 61.6

Find 56 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGCCTGGTTTGGAATT+AGG 0.117190 7:-31216639 MsaT036295.1:CDS
ATGTTAGTTCTACAAGTTTA+TGG 0.160920 7:-31216857 MsaT036295.1:CDS
GATGGGCCTGGTGGGCTTGA+TGG 0.187359 7:+31216898 None:intergenic
CAAGGTTCATCTTTGTCTTC+TGG 0.222188 7:-31216957 MsaT036295.1:CDS
TATTATTATTCTGCTGATTC+TGG 0.237820 7:+31216523 None:intergenic
CATCATCAAGTTGGTGGATA+TGG 0.242402 7:-31216831 MsaT036295.1:CDS
TATGCCAACAGGAACAAATT+TGG 0.247795 7:-31216709 MsaT036295.1:CDS
AGTTGAATGATAGGAATAAT+TGG 0.264011 7:-31216929 MsaT036295.1:CDS
AGATGCCTGGTTTGGAATTA+GGG 0.264298 7:-31216638 MsaT036295.1:CDS
ATGGGCCTGGTGGGCTTGAT+GGG 0.294523 7:+31216899 None:intergenic
GCTTCAGCTTTAGCTTCTTC+TGG 0.304919 7:-31216996 MsaT036295.1:CDS
CCTGTTGCAGCTATGATTGA+TGG 0.306906 7:+31217035 None:intergenic
GAATTGTGAGAATCATCTTT+AGG 0.322739 7:+31216498 None:intergenic
TTTCATCAATCATCATCTTC+TGG 0.335762 7:-31216807 MsaT036295.1:CDS
CTTGATGGGCCTGATGGGCC+TGG 0.347126 7:+31216886 None:intergenic
TTTCAACTCACAAGAGAGTT+AGG 0.358109 7:-31217107 MsaT036295.1:CDS
ATGGGCCTGATGGGCCTGGT+GGG 0.388474 7:+31216890 None:intergenic
CTGGTTTGCAGTTGAATGAT+AGG 0.398808 7:-31216938 MsaT036295.1:CDS
ATGCCAACAGGAACAAATTT+GGG 0.405289 7:-31216708 MsaT036295.1:CDS
TCAAGCCCACCAGGCCCATC+AGG 0.414777 7:-31216895 MsaT036295.1:CDS
ATCATAGCTGCAACAGGAAC+AGG 0.443120 7:-31217029 MsaT036295.1:CDS
ACTCAGATTTATCAGCAGAT+TGG 0.447988 7:-31216570 MsaT036295.1:CDS
TCTGGATTTGATATGCCAAC+AGG 0.449552 7:-31216720 MsaT036295.1:CDS
ACTCCCAAATTTGTTCCTGT+TGG 0.452759 7:+31216705 None:intergenic
GTTGAATGATAGGAATAATT+GGG 0.466051 7:-31216928 MsaT036295.1:CDS
AAATCAGCAGATGCCTGGTT+TGG 0.470684 7:-31216646 MsaT036295.1:CDS
AACTAACATGATGACCTTGA+TGG 0.471715 7:+31216871 None:intergenic
ATGACCTTGATGGGCCTGAT+GGG 0.473600 7:+31216881 None:intergenic
CCATCAATCATAGCTGCAAC+AGG 0.479395 7:-31217035 MsaT036295.1:CDS
GATGGGCCTGATGGGCCTGG+TGG 0.480862 7:+31216889 None:intergenic
TTGTCACAAGATGGACATAT+TGG 0.480930 7:-31216615 MsaT036295.1:CDS
GAATTAGGGTTGTCACAAGA+TGG 0.488895 7:-31216624 MsaT036295.1:CDS
ACTGTTGGTGAAAATAATAG+TGG 0.505174 7:-31216768 MsaT036295.1:CDS
TGGCCTCATCATCAAGTTGG+TGG 0.511204 7:-31216837 MsaT036295.1:CDS
GGTGCAAATCAGCAGATGCC+TGG 0.519917 7:-31216651 MsaT036295.1:CDS
TCAGACCCATCAAGCCCACC+AGG 0.525810 7:-31216904 MsaT036295.1:CDS
CAGATGGTGAAACAATTCAA+TGG 0.529264 7:-31217076 MsaT036295.1:CDS
AGATTGGTCAAAATCAAACT+AGG 0.530078 7:-31216554 MsaT036295.1:CDS
CAGGCCCATCAGGCCCATCA+AGG 0.530139 7:-31216885 MsaT036295.1:CDS
ATTCTTGCTGCACATAAAGC+TGG 0.536084 7:+31217131 None:intergenic
AACAGGAACAAATTTGGGAG+TGG 0.545963 7:-31216703 MsaT036295.1:CDS
GGTTTAGTAGCTACTACTGT+TGG 0.551441 7:-31216783 MsaT036295.1:CDS
GATGACCTTGATGGGCCTGA+TGG 0.557322 7:+31216880 None:intergenic
ACTAACATGATGACCTTGAT+GGG 0.557914 7:+31216872 None:intergenic
CATCAATCATCATCTTCTGG+TGG 0.564429 7:-31216804 MsaT036295.1:CDS
GGAACAAATTTGGGAGTGGG+AGG 0.568972 7:-31216699 MsaT036295.1:CDS
GAAGAAGCTAAAGCTGAAGC+TGG 0.576280 7:+31216999 None:intergenic
GGTAATACTTTGACACCACA+AGG 0.588387 7:-31216975 MsaT036295.1:CDS
ACAGGAACAAATTTGGGAGT+GGG 0.594534 7:-31216702 MsaT036295.1:CDS
GATTGGTCAAAATCAAACTA+GGG 0.595101 7:-31216553 MsaT036295.1:CDS
GAACAAATTTGGGAGTGGGA+GGG 0.598189 7:-31216698 MsaT036295.1:CDS
TTATGGCCTCATCATCAAGT+TGG 0.605767 7:-31216840 MsaT036295.1:CDS
GAGTTAGGTCATAAATCAGA+TGG 0.610512 7:-31217092 MsaT036295.1:CDS
TATCCACCAACTTGATGATG+AGG 0.610804 7:+31216834 None:intergenic
GACAACCCTAATTCCAAACC+AGG 0.628289 7:+31216633 None:intergenic
GAAGACAAAGATGAACCTTG+TGG 0.735014 7:+31216960 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 31216488 31217156 31216488 ID=MsaG036295
Chr7 mRNA 31216488 31217156 31216488 ID=MsaT036295.1;Parent=MsaG036295
Chr7 exon 31216488 31217156 31216488 ID=MsaT036295.1.exon1;Parent=MsaT036295.1
Chr7 CDS 31216488 31217156 31216488 ID=cds.MsaT036295.1;Parent=MsaT036295.1
Gene Sequence

>MsaT036295.1

ATGCCAGCTTTATGTGCAGCAAGAATCTTTCAACTCACAAGAGAGTTAGGTCATAAATCAGATGGTGAAACAATTCAATGGCTTTTACAACAATCTGAACCATCAATCATAGCTGCAACAGGAACAGGAACAATACCAGCTTCAGCTTTAGCTTCTTCTGGTAATACTTTGACACCACAAGGTTCATCTTTGTCTTCTGGTTTGCAGTTGAATGATAGGAATAATTGGGCTCAGACCCATCAAGCCCACCAGGCCCATCAGGCCCATCAAGGTCATCATGTTAGTTCTACAAGTTTATGGCCTCATCATCAAGTTGGTGGATATGGATTTCATCAATCATCATCTTCTGGTGGTTTAGTAGCTACTACTGTTGGTGAAAATAATAGTGGAAATTATTTTCAGAAAATTGGGTTTTCTGGATTTGATATGCCAACAGGAACAAATTTGGGAGTGGGAGGGATGAGTTTTACATCAATTTTGGGGGGTGCAAATCAGCAGATGCCTGGTTTGGAATTAGGGTTGTCACAAGATGGACATATTGGTGTGTTGAATCAACAAGCTTTGACTCAGATTTATCAGCAGATTGGTCAAAATCAAACTAGGGTTCAGCACCAGAATCAGCAGAATAATAATACTCCTAAAGATGATTCTCACAATTCAGAACAGTAG

Protein sequence

>MsaT036295.1

MPALCAARIFQLTRELGHKSDGETIQWLLQQSEPSIIAATGTGTIPASALASSGNTLTPQGSSLSSGLQLNDRNNWAQTHQAHQAHQAHQGHHVSSTSLWPHHQVGGYGFHQSSSSGGLVATTVGENNSGNYFQKIGFSGFDMPTGTNLGVGGMSFTSILGGANQQMPGLELGLSQDGHIGVLNQQALTQIYQQIGQNQTRVQHQNQQNNNTPKDDSHNSEQ*