AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036304

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036304 MtrunA17_Chr2g0295731 72.917 96 20 1 9 98 51 146 6.66e-42 140
MsaG036304 MtrunA17_Chr2g0295711 71.717 99 22 1 6 98 47 145 1.06e-40 133
MsaG036304 MtrunA17_Chr2g0296111 68.687 99 25 1 6 98 40 138 8.71e-39 132
MsaG036304 MtrunA17_Chr2g0296151 67.677 99 26 1 6 98 41 139 4.80e-37 128
MsaG036304 MtrunA17_Chr2g0295751 66.667 99 27 1 6 98 48 146 1.58e-35 125
MsaG036304 MtrunA17_Chr2g0295741 67.677 99 26 1 6 98 47 145 1.63e-35 124
MsaG036304 MtrunA17_Chr2g0296121 63.636 99 30 1 6 98 127 225 1.37e-33 120
MsaG036304 MtrunA17_Chr2g0295761 52.083 96 42 1 7 98 62 157 3.21e-29 107
MsaG036304 MtrunA17_Chr2g0296251 54.639 97 40 1 6 98 33 129 1.02e-27 103
MsaG036304 MtrunA17_Chr2g0296561 54.167 96 40 1 7 98 38 133 1.27e-27 98.2
MsaG036304 MtrunA17_Chr2g0296711 54.082 98 39 1 7 98 55 152 1.88e-27 103
MsaG036304 MtrunA17_Chr2g0296301 51.546 97 43 1 6 98 37 133 2.46e-27 102
MsaG036304 MtrunA17_Chr2g0296221 55.102 98 38 1 7 98 81 178 2.50e-27 103
MsaG036304 MtrunA17_Chr2g0296171 54.082 98 39 1 7 98 62 159 3.13e-27 102
MsaG036304 MtrunA17_Chr4g0067121 54.082 98 40 2 6 98 52 149 3.38e-27 102
MsaG036304 MtrunA17_Chr2g0296191 55.102 98 38 1 7 98 664 761 4.10e-27 103
MsaG036304 MtrunA17_Chr2g0296191 50.515 97 44 1 6 98 183 279 1.73e-23 93.2
MsaG036304 MtrunA17_Chr2g0296181 53.061 98 40 1 7 98 53 150 1.16e-26 100
MsaG036304 MtrunA17_Chr2g0315731 54.082 98 39 1 7 98 72 169 2.96e-26 100
MsaG036304 MtrunA17_Chr2g0296721 54.082 98 39 1 7 98 52 149 5.66e-26 99.0
MsaG036304 MtrunA17_Chr2g0296291 52.632 95 41 1 6 96 33 127 3.34e-25 97.1
MsaG036304 MtrunA17_Chr2g0296231 51.020 98 42 2 7 98 51 148 1.88e-24 94.7
MsaG036304 MtrunA17_Chr2g0286191 47.423 97 47 1 6 98 32 128 7.77e-24 92.8
MsaG036304 MtrunA17_Chr4g0067671 48.454 97 46 1 6 98 45 141 3.04e-22 88.6
MsaG036304 MtrunA17_Chr2g0296741 45.918 98 47 1 7 98 72 169 4.12e-22 88.6
MsaG036304 MtrunA17_Chr4g0067141 43.299 97 51 1 6 98 45 141 5.30e-20 82.4
MsaG036304 MtrunA17_Chr2g0296211 46.479 71 32 1 7 71 119 189 2.62e-12 61.2
MsaG036304 MtrunA17_Chr4g0001831 41.772 79 42 1 12 86 44 122 2.69e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036304 AT1G21150.1 41.414 99 54 2 4 98 54 152 3.21e-17 75.5
MsaG036304 AT1G21150.2 41.414 99 54 2 4 98 118 216 4.20e-17 75.1
MsaG036304 AT1G62085.1 37.755 98 57 1 5 98 54 151 4.58e-17 75.1
MsaG036304 AT1G62085.2 37.755 98 57 1 5 98 54 151 4.58e-17 75.1
MsaG036304 AT1G61980.3 37.113 97 57 1 6 98 52 148 5.23e-17 74.7
MsaG036304 AT1G61980.2 37.113 97 57 1 6 98 52 148 5.23e-17 74.7
MsaG036304 AT1G61980.1 37.113 97 57 1 6 98 52 148 5.23e-17 74.7
MsaG036304 AT1G21150.3 41.237 97 53 2 6 98 20 116 1.29e-16 73.6
MsaG036304 AT1G62120.1 38.542 96 55 1 7 98 57 152 2.73e-16 72.8
MsaG036304 AT3G46950.1 37.113 97 57 1 6 98 52 148 1.35e-15 70.9
MsaG036304 AT5G23930.1 38.144 97 56 1 6 98 55 151 2.14e-15 70.5
MsaG036304 AT1G61970.2 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61970.7 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61970.6 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61970.3 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61970.5 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61970.4 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61970.1 36.458 96 57 1 7 98 53 148 3.00e-15 69.7
MsaG036304 AT1G61990.2 37.500 96 56 1 7 98 53 148 1.36e-14 67.8
MsaG036304 AT1G61990.1 37.500 96 56 1 7 98 53 148 1.36e-14 67.8
MsaG036304 AT1G62150.1 35.417 96 58 1 7 98 59 154 5.81e-14 66.2
MsaG036304 AT1G61960.1 34.375 96 59 1 7 98 53 148 6.03e-14 66.2
MsaG036304 AT1G62110.1 34.375 96 59 1 7 98 53 148 4.82e-13 63.5
MsaG036304 AT5G07900.1 33.673 98 61 1 5 98 68 165 2.19e-12 61.6

Find 20 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.168369 7:-31549974 MsaT036304.1:CDS
GGTGGACTTGGAACGAAATT+TGG 0.191925 7:+31549931 None:intergenic
AGGCTTACCTACAAGACTTT+AGG 0.243880 7:+31549719 None:intergenic
AAACTTTGGCAACACGGTTT+TGG 0.279663 7:+31549802 None:intergenic
GGCTTACCTACAAGACTTTA+GGG 0.297356 7:+31549720 None:intergenic
CTCAAATTCTTTAGAAATCA+TGG 0.303416 7:-31549890 MsaT036304.1:CDS
AAGAATTTGAGAACTGAATC+AGG 0.344102 7:+31549902 None:intergenic
TAAGAACCCTAAAGTCTTGT+AGG 0.349158 7:-31549726 None:intergenic
GTCAGAGGTAGAAGCGCCTT+TGG 0.402517 7:+31549766 None:intergenic
TGGCAATCATATCGCGTAGT+TGG 0.438979 7:+31549855 None:intergenic
GGCAATCATATCGCGTAGTT+GGG 0.453430 7:+31549856 None:intergenic
TGAAAGCAGCTCTGGCATCT+TGG 0.471973 7:+31549835 None:intergenic
GGTTTGCATGAAAGCAGCTC+TGG 0.519314 7:+31549827 None:intergenic
CTTTAGGGTTCTTACTGACA+AGG 0.533379 7:+31549735 None:intergenic
AAAAGCCAAAATTGTTGATG+AGG 0.561539 7:+31549969 None:intergenic
GCCAAAATTGTTGATGAGGT+AGG 0.577466 7:+31549973 None:intergenic
AAATTGAAACTTTGGCAACA+CGG 0.599934 7:+31549796 None:intergenic
AAAATTGTTGATGAGGTAGG+AGG 0.631900 7:+31549976 None:intergenic
AAATTTGGAAGCAGATTGTG+AGG 0.633510 7:+31549946 None:intergenic
GACAAGGTTAACAATGTCAG+AGG 0.654821 7:+31549751 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 31549727 31551138 31549727 ID=MsaG036304
Chr7 mRNA 31549727 31551138 31549727 ID=MsaT036304.1;Parent=MsaG036304
Chr7 exon 31549727 31549998 31549727 ID=MsaT036304.1.exon2;Parent=MsaT036304.1
Chr7 CDS 31549727 31549998 31549727 ID=cds.MsaT036304.1;Parent=MsaT036304.1
Chr7 exon 31551114 31551138 31551114 ID=MsaT036304.1.exon1;Parent=MsaT036304.1
Chr7 CDS 31551114 31551138 31551114 ID=cds.MsaT036304.1;Parent=MsaT036304.1
Gene Sequence

>MsaT036304.1

ATGGGACTTTTATTAGCATTTTTCGCCTCCTACCTCATCAACAATTTTGGCTTTTCCTCACAATCTGCTTCCAAATTTCGTTCCAAGTCCACCCAAAAACCTGATTCAGTTCTCAAATTCTTTAGAAATCATGGTTTATCTGATTCCCAACTACGCGATATGATTGCCAAGATGCCAGAGCTGCTTTCATGCAAACCCTCCAAAACCGTGTTGCCAAAGTTTCAATTTTTTCACTCCAAAGGCGCTTCTACCTCTGACATTGTTAACCTTGTCAGTAAGAACCCTAAAGTCTTGTAG
Protein sequence

>MsaT036304.1

MGLLLAFFASYLINNFGFSSQSASKFRSKSTQKPDSVLKFFRNHGLSDSQLRDMIAKMPELLSCKPSKTVLPKFQFFHSKGASTSDIVNLVSKNPKVL*