AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036335


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036335 MtrunA17_Chr7g0225371 96.443 253 9 0 1 253 1 253 0.0 511
MsaG036335 MtrunA17_Chr7g0225251 96.316 190 7 0 64 253 3 192 5.82e-136 380
MsaG036335 MtrunA17_Chr7g0225341 78.378 111 22 2 136 245 8 117 1.19e-52 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036335 AT5G14140.1 63.158 247 82 4 11 249 14 259 4.10e-111 320

Find 58 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTCATGATTCTTTCTTTC+AGG 0.183557 7:+32222723 MsaT036335.1:CDS
GAAGTCTTCTGTCCCATTGC+TGG 0.271752 7:+32222574 MsaT036335.1:CDS
CAAAACGGAAAATTTGAATC+TGG 0.300941 7:-32221371 None:intergenic
GGGTTGGAACTTACGTGAAA+TGG 0.341316 7:-32221399 None:intergenic
TGCTGGGTGTGGAGCGCGTT+TGG 0.354156 7:+32222591 MsaT036335.1:CDS
TTTATCCTGTGTTTCTTTGA+AGG 0.386367 7:-32223802 None:intergenic
TTCTGTCCCATTGCTGGGTG+TGG 0.397837 7:+32222580 MsaT036335.1:CDS
ATGACATGCCTTTGGTGTTA+CGG 0.399250 7:-32223966 None:intergenic
ACGAGAACTTCTTGTCAAAC+AGG 0.415459 7:+32221454 MsaT036335.1:CDS
TCTCAAAAGGAGGATACTTC+AGG 0.418130 7:+32223844 MsaT036335.1:CDS
TGACAAGAAGTTCTCGTTCA+AGG 0.428214 7:-32221448 None:intergenic
CGTGAAATGGGTTTCCAAAA+CGG 0.438517 7:-32221386 None:intergenic
CGCGCTCCACACCCAGCAAT+GGG 0.438523 7:-32222586 None:intergenic
ACTCCTTCCTCTATCAGCTT+TGG 0.443914 7:+32223940 MsaT036335.1:CDS
GGTTGGAACTTACGTGAAAT+GGG 0.448158 7:-32221398 None:intergenic
GACTCTAGAACACACAGAAC+AGG 0.456459 7:-32222662 None:intergenic
AAGTCTTCTGTCCCATTGCT+GGG 0.459510 7:+32222575 MsaT036335.1:CDS
GGAGGATACTTCAGGAATGA+TGG 0.477569 7:+32223852 MsaT036335.1:CDS
AGGGACAAATGACATGCCTT+TGG 0.478422 7:-32223974 None:intergenic
AAAGAAAGAATCATGAGCTT+CGG 0.485050 7:-32222719 None:intergenic
GATAGCGAAGAAAGGTGAAT+CGG 0.497649 7:-32221420 None:intergenic
AATTCAAAAGCTACAAAAGT+AGG 0.497972 7:+32223716 MsaT036335.1:CDS
CGAGCTGTTCAGCGTGGGAA+AGG 0.500378 7:+32223997 MsaT036335.1:CDS
GTAAGCTTATTTCTTAGTAA+CGG 0.507580 7:-32224037 None:intergenic
ACAAGAGAAGGAACAGTTTG+AGG 0.507962 7:+32221609 MsaT036335.1:CDS
GATTCACCTTTCTTCGCTAT+CGG 0.511476 7:+32221422 MsaT036335.1:CDS
TTAATTTGAAAAGGTTGCGT+TGG 0.517594 7:+32221573 MsaT036335.1:intron
GATATAGTATGAATGCTTAG+TGG 0.520599 7:+32223678 MsaT036335.1:intron
ACGCGCTCCACACCCAGCAA+TGG 0.530230 7:-32222587 None:intergenic
TAGTATGAATGCTTAGTGGA+AGG 0.549245 7:+32223682 MsaT036335.1:intron
CGAAGAAAGGTGAATCGGGT+TGG 0.552955 7:-32221415 None:intergenic
ATTTGAATTAACACAAGAGA+AGG 0.553168 7:+32221597 MsaT036335.1:CDS
CTCTGCCTTCAAAGAAACAC+AGG 0.556458 7:+32223797 MsaT036335.1:CDS
ACGGCGACCAAAGCTGATAG+AGG 0.559633 7:-32223947 None:intergenic
TACTTCAGGAATGATGGAAG+TGG 0.560728 7:+32223858 MsaT036335.1:CDS
GGAAGTGGAAAATGCAGCTA+TGG 0.560741 7:+32223873 MsaT036335.1:CDS
TCCCTCGAGCTGTTCAGCGT+GGG 0.564627 7:+32223992 MsaT036335.1:CDS
ATAGCGAAGAAAGGTGAATC+GGG 0.565377 7:-32221419 None:intergenic
CGTGGGAAAGGATCTAACTC+TGG 0.568460 7:+32224009 MsaT036335.1:CDS
GTCCCTCGAGCTGTTCAGCG+TGG 0.579249 7:+32223991 MsaT036335.1:CDS
TTCCCACGCTGAACAGCTCG+AGG 0.579646 7:-32223994 None:intergenic
ACTTGATACAAGAGGATGGA+AGG 0.580239 7:+32221632 MsaT036335.1:CDS
AGGTTACCGATAGCGAAGAA+AGG 0.582602 7:-32221428 None:intergenic
GGTTGAACTAACGATGGAAG+TGG 0.593198 7:+32221337 None:intergenic
AGTATGAATGCTTAGTGGAA+GGG 0.595383 7:+32223683 MsaT036335.1:intron
GAATGCTTAGTGGAAGGGTG+CGG 0.598441 7:+32223688 MsaT036335.1:CDS
CGACCAAAGCTGATAGAGGA+AGG 0.601809 7:-32223943 None:intergenic
TATGCTGAGCAAACGTGATG+TGG 0.604052 7:-32222689 None:intergenic
TTTCAGGCAAAAGTTGCTCG+TGG 0.610110 7:+32222739 MsaT036335.1:CDS
GAGGACTTGATACAAGAGGA+TGG 0.612431 7:+32221628 MsaT036335.1:CDS
GTTTGAGGACTTGATACAAG+AGG 0.614127 7:+32221624 MsaT036335.1:CDS
TTTGGTCGCCGTAACACCAA+AGG 0.617378 7:+32223958 MsaT036335.1:CDS
ATGCTGAGCAAACGTGATGT+GGG 0.625974 7:-32222688 None:intergenic
AGTTGCTCGTGGCTATGACA+TGG 0.638718 7:+32222750 MsaT036335.1:CDS
TCCCACGCTGAACAGCTCGA+GGG 0.661255 7:-32223993 None:intergenic
AAAGCTGATAGAGGAAGGAG+TGG 0.665134 7:-32223938 None:intergenic
ATCTAGATACTGCTGAAACG+AGG 0.692577 7:-32223900 None:intergenic
TGCTGAGCAAACGTGATGTG+GGG 0.701816 7:-32222687 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 32221350 32224053 32221350 ID=MsaG036335
Chr7 mRNA 32221350 32224053 32221350 ID=MsaT036335.1;Parent=MsaG036335
Chr7 exon 32221350 32221475 32221350 ID=MsaT036335.1.exon1;Parent=MsaT036335.1
Chr7 CDS 32221350 32221475 32221350 ID=cds.MsaT036335.1;Parent=MsaT036335.1
Chr7 exon 32221586 32221653 32221586 ID=MsaT036335.1.exon2;Parent=MsaT036335.1
Chr7 CDS 32221586 32221653 32221586 ID=cds.MsaT036335.1;Parent=MsaT036335.1
Chr7 exon 32222573 32222771 32222573 ID=MsaT036335.1.exon3;Parent=MsaT036335.1
Chr7 CDS 32222573 32222771 32222573 ID=cds.MsaT036335.1;Parent=MsaT036335.1
Chr7 exon 32223685 32224053 32223685 ID=MsaT036335.1.exon4;Parent=MsaT036335.1
Chr7 CDS 32223685 32224053 32223685 ID=cds.MsaT036335.1;Parent=MsaT036335.1
Gene Sequence

>MsaT036335.1

ATGGAAGTGGAGAATCGAAATCCAGATTCAAATTTTCCGTTTTGGAAACCCATTTCACGTAAGTTCCAACCCGATTCACCTTTCTTCGCTATCGGTAACCTTGAACGAGAACTTCTTGTCAAACAGGTTGCGTTGGAATTTGAATTAACACAAGAGAAGGAACAGTTTGAGGACTTGATACAAGAGGATGGAAGGGAAGTCTTCTGTCCCATTGCTGGGTGTGGAGCGCGTTTGGCTTCAATTGAGAATTTTGAAAATCACTATAATGCAAGACATACTGCATCCTGTTCTGTGTGTTCTAGAGTCTACCCCACATCACGTTTGCTCAGCATACATGTATCCGAAGCTCATGATTCTTTCTTTCAGGCAAAAGTTGCTCGTGGCTATGACATGTATGAATGCTTAGTGGAAGGGTGCGGTTTGAAATTCAAAAGCTACAAAAGTAGGCAGCAGCATCTAGTTGACAAGCATAAATTCCCGACGTCATTCGAGTTTTTCAAGAAGGCTCTGCCTTCAAAGAAACACAGGATAAAGTCTCAACGCAAAAAACCTTCTCAAAAGGAGGATACTTCAGGAATGATGGAAGTGGAAAATGCAGCTATGGATGACCTCGTTTCAGCAGTATCTAGATTAAATACTTCAGACACCACTCCTTCCTCTATCAGCTTTGGTCGCCGTAACACCAAAGGCATGTCATTTGTCCCTCGAGCTGTTCAGCGTGGGAAAGGATCTAACTCTGGCTCATCCGTTACTAAGAAATAA

Protein sequence

>MsaT036335.1

MEVENRNPDSNFPFWKPISRKFQPDSPFFAIGNLERELLVKQVALEFELTQEKEQFEDLIQEDGREVFCPIAGCGARLASIENFENHYNARHTASCSVCSRVYPTSRLLSIHVSEAHDSFFQAKVARGYDMYECLVEGCGLKFKSYKSRQQHLVDKHKFPTSFEFFKKALPSKKHRIKSQRKKPSQKEDTSGMMEVENAAMDDLVSAVSRLNTSDTTPSSISFGRRNTKGMSFVPRAVQRGKGSNSGSSVTKK*