AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036455


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036455 MtrunA17_Chr7g0226451 89.627 241 14 3 1 235 117 352 8.32e-146 410
MsaG036455 MtrunA17_Chr5g0431351 39.844 128 42 3 108 234 123 216 2.21e-21 88.6
MsaG036455 MtrunA17_Chr1g0209861 41.860 129 52 4 109 234 316 424 1.60e-19 86.7
MsaG036455 MtrunA17_Chr8g0390971 37.126 167 55 7 76 234 276 400 2.54e-19 85.9
MsaG036455 MtrunA17_Chr4g0071811 39.669 121 44 3 114 234 293 384 7.22e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036455 AT5G14370.1 54.054 185 56 9 56 235 177 337 3.23e-43 149
MsaG036455 AT5G57180.2 43.548 124 35 3 111 234 337 425 7.55e-23 96.3
MsaG036455 AT1G73870.1 42.963 135 46 5 108 234 276 387 6.49e-22 93.2
MsaG036455 AT4G25990.1 42.400 125 37 3 111 234 293 383 9.72e-21 90.1
MsaG036455 AT1G25440.1 42.188 128 55 4 109 234 293 403 7.37e-20 87.8
MsaG036455 AT1G07050.1 42.188 128 36 4 109 235 104 194 1.69e-19 83.6
MsaG036455 AT5G57180.1 41.525 118 34 3 111 228 337 419 8.07e-19 84.7
MsaG036455 AT1G68520.1 37.879 132 57 4 108 234 288 399 4.42e-17 79.7
MsaG036455 AT4G25990.2 36.111 144 34 3 111 234 293 398 4.43e-17 79.7
MsaG036455 AT4G25990.3 38.211 123 33 2 111 228 293 377 9.53e-17 78.6
MsaG036455 AT1G07050.2 38.843 121 36 4 109 228 104 187 3.21e-14 69.3
MsaG036455 AT1G49130.1 68.889 45 14 0 190 234 276 320 3.30e-14 71.2
MsaG036455 AT1G49130.2 68.889 45 14 0 190 234 269 313 3.47e-14 70.9
MsaG036455 AT2G46670.1 43.284 67 34 1 171 233 107 173 2.13e-11 61.2
MsaG036455 AT2G46790.5 43.284 67 34 1 171 233 235 301 2.98e-11 62.4
MsaG036455 AT2G46790.4 43.284 67 34 1 171 233 235 301 2.98e-11 62.4
MsaG036455 AT2G46790.2 43.284 67 34 1 171 233 275 341 6.07e-11 61.6
MsaG036455 AT2G46790.1 43.284 67 34 1 171 233 392 458 8.89e-11 61.6
MsaG036455 AT2G24790.1 62.222 45 17 0 190 234 227 271 9.06e-11 60.8

Find 60 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTATTTGTACAGGTAATA+TGG 0.161633 7:-34658609 MsaT036455.1:intron
GGTTATGGGTGTGATGTTAA+TGG 0.287554 7:-34658583 MsaT036455.1:CDS
TTGTTAGCTCCAACAACTTC+AGG 0.323703 7:+34658544 None:intergenic
TTGAATGCTTGGTCTGATAA+AGG 0.338505 7:-34660051 MsaT036455.1:CDS
CAGGTAATATGGGATGGTTA+TGG 0.362688 7:-34658598 MsaT036455.1:CDS
AGGTAATATGGGATGGTTAT+GGG 0.363177 7:-34658597 MsaT036455.1:CDS
TGAAGAGAGTGAAGAGAGAT+GGG 0.373318 7:-34660202 MsaT036455.1:CDS
AAAGGTTCTCTTTATGTTGA+TGG 0.373469 7:-34660033 MsaT036455.1:CDS
CAGACTGTTCCTGATTTGTT+CGG 0.385801 7:-34659994 MsaT036455.1:CDS
GGTATTTGTACAGGTAATAT+GGG 0.387282 7:-34658608 MsaT036455.1:intron
TGTGAAGGTGAAGGAAGAAA+TGG 0.408295 7:-34658515 MsaT036455.1:CDS
GACGGTGGAGCAACGCTAAA+CGG 0.415141 7:-34660366 MsaT036455.1:CDS
ATGAAGAGAGTGAAGAGAGA+TGG 0.416748 7:-34660203 MsaT036455.1:CDS
GAAGAAATGGGTTGGAAGCT+TGG 0.419659 7:-34658502 MsaT036455.1:CDS
AAGAAATGGGTTGGAAGCTT+GGG 0.434227 7:-34658501 MsaT036455.1:CDS
ATTGAACCACCGAACAAATC+AGG 0.449972 7:+34659985 None:intergenic
GGAAAGCAATGAGGAGGAAG+AGG 0.454954 7:-34660233 MsaT036455.1:CDS
GTTATGGGTGTGATGTTAAT+GGG 0.457104 7:-34658582 MsaT036455.1:CDS
GAAGTTAATAGTACTTGTGT+TGG 0.464035 7:-34660132 MsaT036455.1:CDS
AGGTGTCCGGTGATGGATCA+AGG 0.465230 7:-34660264 MsaT036455.1:CDS
TGGAAAGTTCCTGAAGTTGT+TGG 0.467176 7:-34658553 MsaT036455.1:CDS
AGAGACGAAGAAGGAAATGA+TGG 0.504022 7:-34660157 MsaT036455.1:CDS
ATTAGTAAAATTACATTGGG+AGG 0.505856 7:+34659961 None:intergenic
AGTGATGAGGTGTCCGGTGA+TGG 0.506901 7:-34660271 MsaT036455.1:CDS
AGTTGTTGAAGAGACGAAGA+AGG 0.513266 7:-34660166 MsaT036455.1:CDS
GATGACGACGACGGAGGAAG+AGG 0.514002 7:-34660307 MsaT036455.1:CDS
TTAGTAAAATTACATTGGGA+GGG 0.521925 7:+34659962 None:intergenic
GCAGGGACGATTTGTGAAGA+GGG 0.527154 7:-34656582 MsaT036455.1:intron
ATGATGGGATATTGAATGCT+TGG 0.532795 7:-34660062 MsaT036455.1:CDS
TGTTGAGATACAAAGAGAAG+AGG 0.545019 7:-34658462 MsaT036455.1:CDS
TCAACTTGCGAACTTCATAA+CGG 0.545911 7:+34658413 None:intergenic
TTATGTTGATGGTTGTGATG+AGG 0.548680 7:-34660022 MsaT036455.1:CDS
TCCGGTGATGGATCAAGGGT+TGG 0.551320 7:-34660259 MsaT036455.1:CDS
ACTGTTCCTGATTTGTTCGG+TGG 0.551576 7:-34659991 MsaT036455.1:CDS
TGCAGGGACGATTTGTGAAG+AGG 0.561388 7:-34656583 MsaT036455.1:intron
GTTTGAAGCTTGATCATGAT+GGG 0.563359 7:-34660077 MsaT036455.1:CDS
AGTTTGAAGCTTGATCATGA+TGG 0.572212 7:-34660078 MsaT036455.1:CDS
TTTGTACAGGTAATATGGGA+TGG 0.575240 7:-34658604 MsaT036455.1:intron
GACGAAGAAGGAAATGATGG+AGG 0.575278 7:-34660154 MsaT036455.1:CDS
GTGAAGGTGAAGGAAGAAAT+GGG 0.575413 7:-34658514 MsaT036455.1:CDS
ACCAACCCTTGATCCATCAC+CGG 0.584061 7:+34660258 None:intergenic
TGGTGAGGAAAGCAATGAGG+AGG 0.586113 7:-34660239 MsaT036455.1:CDS
AGGTGAAGGAAGAAATGGGT+TGG 0.586587 7:-34658510 MsaT036455.1:CDS
CAATAACAATGTGAAGGTGA+AGG 0.593718 7:-34658524 MsaT036455.1:CDS
AGAAATAGTACCTTCATCCT+CGG 0.597938 7:+34658376 None:intergenic
GACGACGACGGAGGAAGAGG+AGG 0.602906 7:-34660304 MsaT036455.1:CDS
ATTGTCAGTGATGAGGTGTC+CGG 0.606087 7:-34660277 MsaT036455.1:CDS
TGATGGATCAAGGGTTGGTG+AGG 0.611319 7:-34660254 MsaT036455.1:CDS
TTGGAAGCTTGGGCAGAGAG+AGG 0.619401 7:-34658491 MsaT036455.1:CDS
AACAAATCAGGAACAGTCTG+AGG 0.628449 7:+34659997 None:intergenic
AGCTAACAATAACAATGTGA+AGG 0.629964 7:-34658530 MsaT036455.1:CDS
GCGTTACTCTTCACATCAGA+CGG 0.631227 7:-34660384 MsaT036455.1:CDS
AGGAGTCATTGTCAGTGATG+AGG 0.647629 7:-34660284 MsaT036455.1:CDS
GTGATGTTAATGGGAACACA+TGG 0.674682 7:-34658573 MsaT036455.1:CDS
GGTTGGTGAGGAAAGCAATG+AGG 0.677201 7:-34660242 MsaT036455.1:CDS
GGTGTCCGGTGATGGATCAA+GGG 0.677873 7:-34660263 MsaT036455.1:CDS
AGCGGTGATGACGACGACGG+AGG 0.681137 7:-34660313 MsaT036455.1:CDS
TTACTCTTCACATCAGACGG+TGG 0.732316 7:-34660381 MsaT036455.1:CDS
AACAGCGGTGATGACGACGA+CGG 0.737423 7:-34660316 MsaT036455.1:CDS
TGAATCGACTACGTTAACAG+CGG 0.787904 7:-34660331 MsaT036455.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 34656577 34660427 34656577 ID=MsaG036455
Chr7 mRNA 34656577 34660427 34656577 ID=MsaT036455.1;Parent=MsaG036455
Chr7 exon 34656577 34656600 34656577 ID=MsaT036455.1.exon3;Parent=MsaT036455.1
Chr7 CDS 34656577 34656600 34656577 ID=cds.MsaT036455.1;Parent=MsaT036455.1
Chr7 exon 34658387 34658617 34658387 ID=MsaT036455.1.exon2;Parent=MsaT036455.1
Chr7 CDS 34658387 34658617 34658387 ID=cds.MsaT036455.1;Parent=MsaT036455.1
Chr7 exon 34659975 34660427 34659975 ID=MsaT036455.1.exon1;Parent=MsaT036455.1
Chr7 CDS 34659975 34660427 34659975 ID=cds.MsaT036455.1;Parent=MsaT036455.1
Gene Sequence

>MsaT036455.1

ATGCACGAAGAAAACAATGTAGCGTTACTCTTCACATCAGACGGTGGAGCAACGCTAAACGGTCTTCTAGAAAATGAATCGACTACGTTAACAGCGGTGATGACGACGACGGAGGAAGAGGAGGAGTCATTGTCAGTGATGAGGTGTCCGGTGATGGATCAAGGGTTGGTGAGGAAAGCAATGAGGAGGAAGAGGAATGATCATGAAGAGAGTGAAGAGAGATGGGTGAGTTATTCAGAAGTTGTTGAAGAGACGAAGAAGGAAATGATGGAGGAAGTTAATAGTACTTGTGTTGGATTTGAAACGACGTCGTTTTTTGGGTCTTTGAGTTTGAAGCTTGATCATGATGGGATATTGAATGCTTGGTCTGATAAAGGTTCTCTTTATGTTGATGGTTGTGATGAGGCTCCTCAGACTGTTCCTGATTTGTTCGGTGGTTCAATCCCTCCCAATGTAATATGGGATGGTTATGGGTGTGATGTTAATGGGAACACATGGAAAGTTCCTGAAGTTGTTGGAGCTAACAATAACAATGTGAAGGTGAAGGAAGAAATGGGTTGGAAGCTTGGGCAGAGAGAGGCAAGTTTGTTGAGATACAAAGAGAAGAGGCAAAGCAGACTCTTTGCTAAGAGAATCCGTTATGAAGTTCGCAAGTTGAATGCTGAAAAACGGCCGAGGATGAAGGGACGATTTGTGAAGAGGGAATGA

Protein sequence

>MsaT036455.1

MHEENNVALLFTSDGGATLNGLLENESTTLTAVMTTTEEEEESLSVMRCPVMDQGLVRKAMRRKRNDHEESEERWVSYSEVVEETKKEMMEEVNSTCVGFETTSFFGSLSLKLDHDGILNAWSDKGSLYVDGCDEAPQTVPDLFGGSIPPNVIWDGYGCDVNGNTWKVPEVVGANNNNVKVKEEMGWKLGQREASLLRYKEKRQSRLFAKRIRYEVRKLNAEKRPRMKGRFVKRE*