AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036660 MtrunA17_Chr7g0228531 44.175 206 64 6 26 181 194 398 2.49e-41 144
MsaG036660 MtrunA17_Chr7g0228481 44.660 206 63 6 26 181 194 398 8.51e-39 137
MsaG036660 MtrunA17_Chr7g0233061 37.629 194 81 5 21 174 148 341 4.32e-21 88.6
MsaG036660 MtrunA17_Chr3g0117511 45.283 106 46 3 54 147 185 290 1.46e-20 87.8
MsaG036660 MtrunA17_Chr1g0177901 33.929 168 58 3 67 181 20 187 2.61e-20 84.0
MsaG036660 MtrunA17_Chr7g0233151 40.323 124 36 2 96 181 7 130 5.93e-20 82.0
MsaG036660 MtrunA17_Chr7g0228401 34.810 158 50 3 77 181 315 472 5.01e-19 83.6
MsaG036660 MtrunA17_Chr4g0058991 38.261 115 59 3 46 148 203 317 1.09e-18 82.4
MsaG036660 MtrunA17_Chr5g0429661 31.707 164 90 5 12 158 77 235 2.62e-18 80.9
MsaG036660 MtrunA17_Chr6g0460661 38.614 101 52 3 75 169 50 146 5.03e-15 68.9
MsaG036660 MtrunA17_Chr5g0397841 40.860 93 43 3 54 134 112 204 6.68e-15 71.6
MsaG036660 MtrunA17_Chr4g0008551 38.261 115 51 5 55 149 41 155 1.33e-14 70.5
MsaG036660 MtrunA17_Chr7g0238631 41.935 93 42 3 55 135 116 208 7.30e-14 68.9
MsaG036660 MtrunA17_Chr1g0157731 32.174 115 65 4 55 156 94 208 2.52e-13 67.4
MsaG036660 MtrunA17_Chr3g0139671 33.913 115 63 4 55 156 88 202 6.48e-13 66.2
MsaG036660 MtrunA17_Chr5g0417621 35.043 117 59 4 48 147 130 246 2.11e-12 64.3
MsaG036660 MtrunA17_Chr4g0042501 35.345 116 62 4 34 136 86 201 2.26e-12 64.3
MsaG036660 MtrunA17_Chr4g0070641 32.000 100 55 3 49 135 11 110 1.48e-11 60.1
MsaG036660 MtrunA17_Chr7g0233161 44.944 89 37 3 15 91 262 350 1.53e-11 61.6
MsaG036660 MtrunA17_Chr4g0053431 36.559 93 47 3 55 135 8 100 1.96e-11 60.8
MsaG036660 MtrunA17_Chr7g0247131 45.763 59 31 1 77 134 17 75 1.97e-11 60.8
MsaG036660 MtrunA17_Chr3g0133601 35.484 93 48 3 55 135 40 132 2.65e-11 60.1
MsaG036660 MtrunA17_Chr8g0346561 34.043 94 49 3 55 135 17 110 2.89e-11 60.8
MsaG036660 MtrunA17_Chr5g0441131 45.312 64 33 2 77 138 48 111 4.82e-11 58.9
MsaG036660 MtrunA17_Chr7g0270891 40.984 61 35 1 77 136 51 111 5.00e-11 60.5
MsaG036660 MtrunA17_Chr1g0186061 34.066 91 47 3 55 132 25 115 5.08e-11 59.7
MsaG036660 MtrunA17_Chr8g0349261 45.763 59 31 1 77 134 53 111 5.11e-11 60.1
MsaG036660 MtrunA17_Chr4g0070611 30.833 120 68 4 50 154 12 131 7.01e-11 59.7
MsaG036660 MtrunA17_Chr5g0437321 43.333 60 33 1 77 135 55 114 7.57e-11 59.7
MsaG036660 MtrunA17_Chr4g0055981 32.000 100 53 3 50 135 55 153 7.62e-11 58.9
MsaG036660 MtrunA17_Chr8g0343831 31.313 99 55 3 50 135 8 106 9.07e-11 58.9
MsaG036660 MtrunA17_Chr4g0048511 46.667 60 31 1 77 135 52 111 9.42e-11 59.7
MsaG036660 MtrunA17_Chr4g0044421 43.333 60 33 1 77 135 51 110 9.46e-11 58.2
MsaG036660 MtrunA17_Chr7g0274581 42.029 69 37 2 77 142 51 119 9.84e-11 58.5
MsaG036660 MtrunA17_Chr1g0193801 40.984 61 35 1 77 136 51 111 1.00e-10 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036660 AT4G18770.1 40.000 115 57 3 46 148 211 325 1.27e-20 88.2
MsaG036660 AT3G27785.1 40.541 111 54 2 46 144 183 293 1.57e-19 85.1
MsaG036660 AT5G40360.1 40.678 118 56 3 44 148 150 266 1.73e-18 82.0
MsaG036660 AT5G58850.1 38.532 109 55 3 49 145 102 210 1.60e-17 79.7
MsaG036660 AT2G25230.1 39.535 129 65 4 44 159 18 146 1.93e-17 77.4
MsaG036660 AT5G11050.1 39.640 111 55 3 47 145 100 210 2.20e-17 79.0
MsaG036660 AT5G40430.1 36.691 139 76 3 46 172 48 186 2.03e-16 75.1
MsaG036660 AT3G53200.2 43.056 72 39 2 62 132 1 71 1.00e-13 67.0
MsaG036660 AT5G11510.3 37.778 90 44 2 55 132 84 173 4.45e-13 67.0
MsaG036660 AT5G11510.1 37.778 90 44 2 55 132 84 173 4.45e-13 67.0
MsaG036660 AT5G11510.4 37.778 90 44 2 55 132 84 173 4.45e-13 67.0
MsaG036660 AT5G11510.2 37.778 90 44 2 55 132 84 173 4.72e-13 67.0
MsaG036660 AT5G11510.5 37.778 90 44 2 55 132 84 173 4.92e-13 67.0
MsaG036660 AT4G33450.1 44.595 74 36 2 77 149 55 124 8.62e-13 65.1
MsaG036660 AT1G69560.1 29.921 127 72 4 44 153 99 225 1.21e-12 65.1
MsaG036660 AT1G69560.2 29.921 127 72 4 44 153 115 241 1.26e-12 65.1
MsaG036660 AT3G28470.1 33.663 101 54 3 48 135 10 110 1.29e-12 65.1
MsaG036660 AT3G53200.1 47.368 57 29 1 77 132 48 104 1.95e-12 63.9
MsaG036660 AT5G02320.3 36.559 93 47 3 55 135 70 162 2.10e-12 65.1
MsaG036660 AT5G02320.1 36.559 93 47 3 55 135 130 222 2.15e-12 65.1
MsaG036660 AT5G02320.2 36.559 93 47 3 55 135 130 222 2.15e-12 65.1
MsaG036660 AT2G47190.1 32.110 109 61 3 37 132 7 115 2.39e-12 63.9
MsaG036660 AT1G57560.2 42.029 69 39 1 77 144 51 119 3.58e-12 63.5
MsaG036660 AT3G09370.2 38.710 93 45 3 55 135 138 230 3.96e-12 64.3
MsaG036660 AT3G09370.1 38.710 93 45 3 55 135 133 225 3.99e-12 63.9
MsaG036660 AT1G57560.1 42.029 69 39 1 77 144 51 119 4.46e-12 63.5
MsaG036660 AT3G09370.3 38.710 93 45 3 55 135 146 238 5.19e-12 63.9
MsaG036660 AT5G35550.1 36.585 82 51 1 77 157 53 134 3.29e-11 60.8
MsaG036660 AT5G35550.2 36.585 82 51 1 77 157 56 137 3.47e-11 60.5
MsaG036660 AT4G01680.2 35.632 87 50 2 62 147 48 129 3.54e-11 61.2
MsaG036660 AT1G09540.1 43.333 60 33 1 77 135 51 110 3.83e-11 61.2
MsaG036660 AT4G28110.1 46.667 60 31 1 77 135 51 110 4.18e-11 60.5
MsaG036660 AT4G32730.1 34.409 93 49 3 55 135 90 182 4.71e-11 61.2
MsaG036660 AT1G26780.1 44.262 61 33 1 77 136 134 194 5.12e-11 60.5
MsaG036660 AT3G55730.1 34.409 93 49 3 55 135 59 151 5.28e-11 60.8
MsaG036660 AT4G32730.5 34.409 93 49 3 55 135 90 182 5.43e-11 60.8
MsaG036660 AT4G32730.4 34.409 93 49 3 55 135 90 182 5.43e-11 60.8
MsaG036660 AT4G32730.3 34.409 93 49 3 55 135 90 182 5.43e-11 60.8
MsaG036660 AT4G32730.2 34.409 93 49 3 55 135 90 182 5.43e-11 60.8
MsaG036660 AT1G17950.1 40.260 77 42 2 77 149 41 117 7.18e-11 59.7
MsaG036660 AT1G26780.2 44.262 61 33 1 77 136 134 194 8.30e-11 60.1
MsaG036660 AT4G12350.1 42.188 64 36 1 73 135 47 110 9.65e-11 59.7
MsaG036660 AT5G56110.1 34.343 99 52 3 49 134 11 109 9.88e-11 59.7

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATTCTCGACTTGCTTAA+TGG 0.229142 7:+40840514 None:intergenic
ACTACTCCAGAAGATGAATT+TGG 0.261057 7:-40840428 MsaT036660.1:CDS
GATCAAGCCCACAACATATT+TGG 0.264284 7:-40840608 MsaT036660.1:CDS
AAAAGAAACACCTTAATATC+TGG 0.291683 7:+40841122 None:intergenic
CCATTGATCAGCATTTATAT+TGG 0.314785 7:+40841383 None:intergenic
GAATAGTATTCTGCTGCTTA+TGG 0.333313 7:+40841436 None:intergenic
CAATCTTAAACCAGATATTA+AGG 0.334007 7:-40841132 MsaT036660.1:intron
TATCCACCAAATTCATCTTC+TGG 0.354153 7:+40840422 None:intergenic
TAAGCAGCAGAATACTATTC+AGG 0.381837 7:-40841433 MsaT036660.1:CDS
TAATCATCGTTGAATGTCAT+TGG 0.440215 7:+40840395 None:intergenic
ACAACATATTTGGAGACAAC+TGG 0.446422 7:-40840598 MsaT036660.1:CDS
GTTGTCTCCAAATATGTTGT+GGG 0.463375 7:+40840601 None:intergenic
TGCTTATGGTAAATGAGAAC+GGG 0.465009 7:+40841450 None:intergenic
ATATTTGTATCCAATTTCAC+TGG 0.483235 7:+40841491 None:intergenic
AATGGACTACAAAAGAGAAT+AGG 0.491834 7:-40841365 MsaT036660.1:intron
AGTTGTCTCCAAATATGTTG+TGG 0.504440 7:+40840600 None:intergenic
GAAATTTCAAGAAGATTGCC+AGG 0.513813 7:-40840572 MsaT036660.1:CDS
CCAATATAAATGCTGATCAA+TGG 0.515462 7:-40841383 MsaT036660.1:CDS
TGAGAACGGGTTCTTGATCA+TGG 0.521600 7:+40841463 None:intergenic
CTGCTTATGGTAAATGAGAA+CGG 0.536086 7:+40841449 None:intergenic
TGGATCTCTTTCTAAATCGA+TGG 0.536456 7:-40841184 MsaT036660.1:CDS
TAAAACAATGCATGGAGAGA+TGG 0.560064 7:-40841157 MsaT036660.1:CDS
ATGGACCTTAAAACAATGCA+TGG 0.570560 7:-40841165 MsaT036660.1:CDS
ACTCCAGAAGATGAATTTGG+TGG 0.588319 7:-40840425 MsaT036660.1:CDS
TAAGGATCATCATATGGATG+AGG 0.593905 7:-40841538 None:intergenic
GAATGAGCCATTGAGTGAGG+AGG 0.611909 7:-40840645 MsaT036660.1:CDS
ACATAACTATCGTTACGTCC+TGG 0.612302 7:+40840554 None:intergenic
TGGTTGCAAAACTAGAGTGA+TGG 0.623138 7:+40841511 None:intergenic
TCGATTTAGAAAGAGATCCA+CGG 0.651610 7:+40841187 None:intergenic
GTAGAATGAGCCATTGAGTG+AGG 0.657428 7:-40840648 MsaT036660.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 40840394 40841547 40840394 ID=MsaG036660
Chr7 mRNA 40840394 40841547 40840394 ID=MsaT036660.1;Parent=MsaG036660
Chr7 exon 40840394 40840666 40840394 ID=MsaT036660.1.exon3;Parent=MsaT036660.1
Chr7 CDS 40840394 40840666 40840394 ID=cds.MsaT036660.1;Parent=MsaT036660.1
Chr7 exon 40841133 40841223 40841133 ID=MsaT036660.1.exon2;Parent=MsaT036660.1
Chr7 CDS 40841133 40841223 40841133 ID=cds.MsaT036660.1;Parent=MsaT036660.1
Chr7 exon 40841366 40841547 40841366 ID=MsaT036660.1.exon1;Parent=MsaT036660.1
Chr7 CDS 40841366 40841547 40841366 ID=cds.MsaT036660.1;Parent=MsaT036660.1
Gene Sequence

>MsaT036660.1

ATGGATGAGGTTAACCATCACTCTAGTTTTGCAACCAGTGAAATTGGATACAAATATCAAGACCATGATCAAGAACCCGTTCTCATTTACCATAAGCAGCAGAATACTATTCAGGAGAATAATGAAATTCATCACAAATATTCCAATATAAATGCTGATCAATGGACTACAAAAGAGAATAGCATTTTTGTCGAATCCGTGGATCTCTTTCTAAATCGATGGACCTTAAAACAATGCATGGAGAGATGGTACAATCTTAAACCAGATATTAAGAATGAGCCATTGAGTGAGGAGGAGCAAAACATACTAGATCAAGCCCACAACATATTTGGAGACAACTGGACTGAAATTTCAAGAAGATTGCCAGGACGTAACGATAGTTATGTTAAGAATCATTTGAACGCCATTAAGCAAGTCGAGAATGCCAAGAAAAAATCCAGGAACATGGTGAAAATTAAGAACCAACCTAATTACATTTTTGAAGGTTTGACTACTCCAGAAGATGAATTTGGTGGATATGTGCCAATGACATTCAACGATGATTAG

Protein sequence

>MsaT036660.1

MDEVNHHSSFATSEIGYKYQDHDQEPVLIYHKQQNTIQENNEIHHKYSNINADQWTTKENSIFVESVDLFLNRWTLKQCMERWYNLKPDIKNEPLSEEEQNILDQAHNIFGDNWTEISRRLPGRNDSYVKNHLNAIKQVENAKKKSRNMVKIKNQPNYIFEGLTTPEDEFGGYVPMTFNDD*