AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036699


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036699 MtrunA17_Chr7g0224601 89.869 306 27 2 1 302 1 306 0.0 534
MsaG036699 MtrunA17_Chr8g0392781 56.418 335 111 2 1 300 11 345 1.27e-121 352
MsaG036699 MtrunA17_Chr3g0099561 62.409 274 103 0 28 301 26 299 2.42e-119 345
MsaG036699 MtrunA17_Chr1g0177151 66.239 234 79 0 14 247 20 253 3.65e-111 322
MsaG036699 MtrunA17_Chr5g0444621 67.157 204 67 0 68 271 60 263 5.68e-89 266
MsaG036699 MtrunA17_Chr6g0477171 67.005 197 65 0 49 245 45 241 1.22e-86 260
MsaG036699 MtrunA17_Chr3g0083811 57.759 116 49 0 176 291 1 116 5.92e-38 130
MsaG036699 MtrunA17_Chr3g0083821 67.368 95 31 0 70 164 36 130 6.48e-38 130
MsaG036699 MtrunA17_Chr5g0444631 86.792 53 7 0 102 154 4 56 1.91e-26 99.0
MsaG036699 MtrunA17_Chr6g0477181 71.429 49 14 0 1 49 11 59 2.12e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036699 AT3G20800.1 57.235 311 122 1 1 300 1 311 1.55e-115 336
MsaG036699 AT5G12980.1 54.074 270 124 0 22 291 29 298 1.42e-100 298
MsaG036699 AT2G32550.2 33.955 268 167 4 31 288 53 320 7.15e-39 139
MsaG036699 AT2G32550.3 34.921 252 138 4 54 288 39 281 8.03e-39 138
MsaG036699 AT2G32550.1 37.374 198 121 2 94 288 29 226 1.37e-36 131

Find 62 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCCCAATTTGAGCATTTA+AGG 0.127092 7:-41632221 MsaT036699.1:CDS
CGGAACTTGCACCTTTATTA+TGG 0.186230 7:-41633683 MsaT036699.1:CDS
CAAGCCTTAAATGCTCAAAT+TGG 0.232859 7:+41632217 None:intergenic
TAAGAGGATTGGGTTATTTC+CGG 0.242539 7:-41633703 MsaT036699.1:intron
ATGAAGTTGCATGAAAATAT+TGG 0.267240 7:-41629075 MsaT036699.1:CDS
CCTTTATTATGGAATTCTTA+TGG 0.279542 7:-41633672 MsaT036699.1:CDS
AAGGAAATCTATGCCTTCTT+TGG 0.298112 7:+41631517 None:intergenic
AATAGAGAAACTAACAGTTT+TGG 0.311116 7:+41631436 None:intergenic
TTTGGGCTGTGGTGCTAGAT+TGG 0.341570 7:+41635293 None:intergenic
GTTCCATGGATGCCATGCTT+TGG 0.343484 7:+41635275 None:intergenic
TGAAGTTGCATGAAAATATT+GGG 0.343625 7:-41629074 MsaT036699.1:CDS
TTCCATGGATGCCATGCTTT+GGG 0.345713 7:+41635276 None:intergenic
CATAATAAAGGTGCAAGTTC+CGG 0.352274 7:+41633684 None:intergenic
GGGCTTGCAATGCATTAAAA+AGG 0.353362 7:-41630995 MsaT036699.1:CDS
CAATGTTCTGGAACTACTTC+AGG 0.355418 7:-41632871 MsaT036699.1:intron
ATTCCCTCCACCTGGTACTA+AGG 0.362304 7:+41629038 None:intergenic
CCATAAGAATTCCATAATAA+AGG 0.371929 7:+41633672 None:intergenic
AACTCGAGTGTGCAATGTTC+TGG 0.374857 7:-41632883 MsaT036699.1:CDS
AACTCAACAATCCTGATCTC+AGG 0.384176 7:-41635240 MsaT036699.1:CDS
TTGGGCTGTGGTGCTAGATT+GGG 0.395336 7:+41635294 None:intergenic
CTTGCTAGTCTTGGAGTCAT+TGG 0.399485 7:-41632198 MsaT036699.1:CDS
CATTTAAGGCTTGCTAGTCT+TGG 0.414086 7:-41632207 MsaT036699.1:CDS
TGCCTACGCAACATGGAAAT+CGG 0.417567 7:-41631470 MsaT036699.1:CDS
CTAACTCGCTTGTCGTTTGA+AGG 0.424446 7:+41632241 None:intergenic
GGATGCCATGCTTTGGGCTG+TGG 0.432887 7:+41635282 None:intergenic
TCGCTTGTCGTTTGAAGGAA+TGG 0.449153 7:+41632246 None:intergenic
TTCAATAACAAGGCGTTCCA+TGG 0.458343 7:+41635261 None:intergenic
TACTATTGAAATATTTGTAC+AGG 0.478947 7:-41633649 MsaT036699.1:intron
AAAATTATGTCAGATGATGA+TGG 0.481246 7:-41631257 MsaT036699.1:CDS
TGCTCTTCGTGTTCTCTCCA+AGG 0.482455 7:-41635212 MsaT036699.1:intron
AGCCCAAAGCATGGCATCCA+TGG 0.487495 7:-41635278 MsaT036699.1:CDS
AGATTCAACAGCGGAATAGT+AGG 0.487502 7:+41632921 None:intergenic
AAGCCTTAAATGCTCAAATT+GGG 0.502413 7:+41632218 None:intergenic
GATGGTTTGATTTATGTTTG+TGG 0.502440 7:-41631239 MsaT036699.1:CDS
TGAAAATATTGGGGTGAACC+AGG 0.503360 7:-41629064 MsaT036699.1:CDS
AAGATGAAACAACTTGGAGC+TGG 0.506447 7:-41629101 MsaT036699.1:CDS
TCACAGGTTAACACCAAAGA+AGG 0.528410 7:-41631530 MsaT036699.1:intron
TCTTGGAGTCATTGGTGCAT+TGG 0.530074 7:-41632190 MsaT036699.1:CDS
CACCTGGTACTAAGGGAACC+TGG 0.533033 7:+41629046 None:intergenic
TCCACTATGCCTACGCAACA+TGG 0.551315 7:-41631477 MsaT036699.1:CDS
TTCAGGAAGATGAAACAACT+TGG 0.555820 7:-41629107 MsaT036699.1:intron
AACCAGGTTCCCTTAGTACC+AGG 0.560044 7:-41629048 MsaT036699.1:CDS
ACTCAACAATCCTGATCTCA+GGG 0.561365 7:-41635239 MsaT036699.1:CDS
TCCATGTTGCGTAGGCATAG+TGG 0.563175 7:+41631476 None:intergenic
GATTGGGATGAAGCAGTTCG+AGG 0.563459 7:+41635310 None:intergenic
TCCGCTGTTGAATCTTACTC+AGG 0.564161 7:-41632913 MsaT036699.1:CDS
GTTCCCTTAGTACCAGGTGG+AGG 0.568228 7:-41629042 MsaT036699.1:CDS
TGCCGATTTCCATGTTGCGT+AGG 0.569945 7:+41631468 None:intergenic
TTCCCTCCACCTGGTACTAA+GGG 0.571710 7:+41629039 None:intergenic
AGATGAAACAACTTGGAGCT+GGG 0.578633 7:-41629100 MsaT036699.1:CDS
TTCCCTTAGTACCAGGTGGA+GGG 0.586677 7:-41629041 MsaT036699.1:CDS
GAATTATCTCACTTCTCAGA+AGG 0.602747 7:+41631498 None:intergenic
AGTCATTGGTGCATTGGTGA+AGG 0.613240 7:-41632184 MsaT036699.1:intron
TATCATTGATTCCCTCCACC+TGG 0.621495 7:+41629030 None:intergenic
TAGCACCACAGCCCAAAGCA+TGG 0.626075 7:-41635287 MsaT036699.1:CDS
AGTTTGATCAATTGATGTGA+AGG 0.628164 7:+41631149 None:intergenic
AACATGGTATTGGAAAGTGT+TGG 0.637520 7:-41631179 MsaT036699.1:CDS
GATTGTTGAGTTCAATAACA+AGG 0.670356 7:+41635251 None:intergenic
GAAGTTGCATGAAAATATTG+GGG 0.686036 7:-41629073 MsaT036699.1:CDS
CAGGTTCCCTTAGTACCAGG+TGG 0.694292 7:-41629045 MsaT036699.1:CDS
TGAGACAAAAGAGAATAACA+TGG 0.703854 7:+41631119 None:intergenic
GCCTGAGTAAGATTCAACAG+CGG 0.773865 7:+41632912 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 41629032 41635359 41629032 ID=MsaG036699
Chr7 mRNA 41629032 41635359 41629032 ID=MsaT036699.1;Parent=MsaG036699
Chr7 exon 41629032 41629124 41629032 ID=MsaT036699.1.exon9;Parent=MsaT036699.1
Chr7 CDS 41629032 41629124 41629032 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41630947 41631016 41630947 ID=MsaT036699.1.exon8;Parent=MsaT036699.1
Chr7 CDS 41630947 41631016 41630947 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41631110 41631300 41631110 ID=MsaT036699.1.exon7;Parent=MsaT036699.1
Chr7 CDS 41631110 41631300 41631110 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41631451 41631546 41631451 ID=MsaT036699.1.exon6;Parent=MsaT036699.1
Chr7 CDS 41631451 41631546 41631451 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41632185 41632291 41632185 ID=MsaT036699.1.exon5;Parent=MsaT036699.1
Chr7 CDS 41632185 41632291 41632185 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41632639 41632681 41632639 ID=MsaT036699.1.exon4;Parent=MsaT036699.1
Chr7 CDS 41632639 41632681 41632639 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41632872 41632961 41632872 ID=MsaT036699.1.exon3;Parent=MsaT036699.1
Chr7 CDS 41632872 41632961 41632872 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41633650 41633721 41633650 ID=MsaT036699.1.exon2;Parent=MsaT036699.1
Chr7 CDS 41633650 41633721 41633650 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Chr7 exon 41635213 41635359 41635213 ID=MsaT036699.1.exon1;Parent=MsaT036699.1
Chr7 CDS 41635213 41635359 41635213 ID=cds.MsaT036699.1;Parent=MsaT036699.1
Gene Sequence

>MsaT036699.1

ATGAATAATGTTGCGTCGTTTGTAAGTCCTCGAACTGCTTCATCCCAATCTAGCACCACAGCCCAAAGCATGGCATCCATGGAACGCCTTGTTATTGAACTCAACAATCCTGATCTCAGGGAAAATGCTCTTCGTGTTCTCTCCAAGAGGATTGGGTTATTTCCGGAACTTGCACCTTTATTATGGAATTCTTATGGTACTATTGAAATATTTGTACAGGAAATAACTTCAATATATCCTACTATTCCGCTGTTGAATCTTACTCAGGCACAATCAACTCGAGTGTGCAATGTTCTGGAACTACTTCAGTGTGTGGCATCTCACCCTGATACAAAAATGTCATTTCTCAATGCTAACATGCCTTTATATTTTTATCCATTCCTTCAAACGACAAGCGAGTTAGCCCAATTTGAGCATTTAAGGCTTGCTAGTCTTGGAGTCATTGGTGCATTGGTGAAGGTTAACACCAAAGAAGGCATAGATTTCCTTCTGAGAAGTGAGATAATTCCACTATGCCTACGCAACATGGAAATCGGCAAAGAATTATCCAAAACTGTTGCAACATTTATAATTCAAAAAATTATGTCAGATGATGATGGTTTGATTTATGTTTGTGGAACAGCAGAACGTTTTTTTGCAGTAGTTCAAGTTTTGAACATGGTATTGGAAAGTGTTGGCAATCAGCCTTCACATCAATTGATCAAACTTCTGATTCCATGTTATTCTCTTTTGTCTCAACATCCTAGGGCTTGCAATGCATTAAAAAGGCGACTTCCAAACATGCTGAAAACTGTAAATTTCATCAATTGCCTTCGCGAAGATGAAACAACTTGGAGCTGGGTAATGAAGTTGCATGAAAATATTGGGGTGAACCAGGTTCCCTTAGTACCAGGTGGAGGGAATCAATGA

Protein sequence

>MsaT036699.1

MNNVASFVSPRTASSQSSTTAQSMASMERLVIELNNPDLRENALRVLSKRIGLFPELAPLLWNSYGTIEIFVQEITSIYPTIPLLNLTQAQSTRVCNVLELLQCVASHPDTKMSFLNANMPLYFYPFLQTTSELAQFEHLRLASLGVIGALVKVNTKEGIDFLLRSEIIPLCLRNMEIGKELSKTVATFIIQKIMSDDDGLIYVCGTAERFFAVVQVLNMVLESVGNQPSHQLIKLLIPCYSLLSQHPRACNALKRRLPNMLKTVNFINCLREDETTWSWVMKLHENIGVNQVPLVPGGGNQ*