AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036703


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036703 MtrunA17_Chr7g0224601 88.406 276 30 1 1 274 1 276 3.43e-168 474
MsaG036703 MtrunA17_Chr1g0177151 64.854 239 82 1 11 249 17 253 3.68e-106 315
MsaG036703 MtrunA17_Chr3g0099561 63.968 247 87 1 28 274 26 270 1.40e-103 310
MsaG036703 MtrunA17_Chr8g0392781 55.987 309 99 3 1 274 11 317 1.77e-103 311
MsaG036703 MtrunA17_Chr5g0444621 66.667 210 70 0 70 279 60 269 1.06e-89 273
MsaG036703 MtrunA17_Chr6g0477171 66.667 198 66 0 50 247 44 241 1.34e-84 259
MsaG036703 MtrunA17_Chr3g0083821 67.021 94 31 0 73 166 37 130 3.04e-36 129
MsaG036703 MtrunA17_Chr3g0083811 58.763 97 40 0 178 274 1 97 4.42e-28 107
MsaG036703 MtrunA17_Chr5g0444631 83.019 53 9 0 104 156 4 56 3.04e-24 95.1
MsaG036703 MtrunA17_Chr2g0323051 93.617 47 3 0 288 334 213 259 1.27e-21 97.4
MsaG036703 MtrunA17_Chr4g0023601 72.000 50 14 0 285 334 137 186 4.56e-18 86.3
MsaG036703 MtrunA17_Chr6g0477181 71.429 49 14 0 1 49 11 59 1.15e-14 69.7
MsaG036703 MtrunA17_Chr8g0363081 70.000 40 12 0 358 397 56 95 4.39e-14 67.8
MsaG036703 MtrunA17_Chr1g0167871 41.772 79 46 0 335 413 541 619 1.14e-12 70.1
MsaG036703 MtrunA17_Chr5g0393521 34.409 93 61 0 335 427 94 186 4.78e-12 66.6
MsaG036703 MtrunA17_Chr5g0417421 34.409 93 61 0 335 427 162 254 1.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036703 AT3G20800.1 58.246 285 106 2 1 274 1 283 8.58e-101 303
MsaG036703 AT5G12980.1 56.746 252 107 1 22 273 29 278 6.20e-93 283
MsaG036703 AT2G32550.3 37.611 226 115 4 56 264 39 255 2.71e-37 137
MsaG036703 AT2G32550.2 37.611 226 115 4 56 264 78 294 7.81e-37 137
MsaG036703 AT2G32550.1 40.698 172 99 2 96 264 29 200 2.80e-34 128
MsaG036703 AT4G15210.2 58.182 55 23 0 280 334 134 188 4.16e-15 77.4
MsaG036703 AT4G15210.3 58.182 55 23 0 280 334 81 135 6.33e-15 77.0
MsaG036703 AT4G15210.4 58.182 55 23 0 280 334 81 135 6.33e-15 77.0
MsaG036703 AT4G15210.1 58.182 55 23 0 280 334 134 188 6.37e-15 77.0
MsaG036703 AT2G32290.1 62.000 50 19 0 285 334 202 251 1.17e-13 73.2

Find 78 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAATAAACCTTTCTTTAA+TGG 0.118021 7:-41831705 MsaT036703.1:CDS
TAGCCCAATTTGAACATTTA+AGG 0.121073 7:-41834852 MsaT036703.1:CDS
ACAATGCTGAATTTGTATTT+TGG 0.151011 7:-41827875 MsaT036703.1:CDS
CAGAACTTGCACCTTTATTA+TGG 0.191101 7:-41836320 MsaT036703.1:CDS
CAAGCCTTAAATGTTCAAAT+TGG 0.198561 7:+41834848 None:intergenic
AGGGAATGTCTTCAGATTAT+TGG 0.267363 7:-41827849 MsaT036703.1:CDS
CATAATAAAGGTGCAAGTTC+TGG 0.267649 7:+41836321 None:intergenic
CCTTTATTATGGAATTCTTA+TGG 0.279542 7:-41836309 MsaT036703.1:CDS
TATCATCAATCATCAGTTCT+AGG 0.300027 7:-41827612 MsaT036703.1:CDS
GCATTACTGCTGATGCTCTT+AGG 0.304459 7:-41827827 MsaT036703.1:CDS
AATAGAGAAACTAACAGTTT+TGG 0.311116 7:+41834060 None:intergenic
TAATGGAAGAACTTCCATTC+AGG 0.344450 7:-41831688 MsaT036703.1:intron
GGGCTTGCAATGCATTAAAA+AGG 0.353362 7:-41833618 MsaT036703.1:CDS
CAATGTTCTGGAACTACTTC+AGG 0.355418 7:-41835503 MsaT036703.1:intron
GGTTTCTCGCGTGATCCTGC+TGG 0.362273 7:-41827591 MsaT036703.1:CDS
TTTGTGCTGTGGTGCTAGAT+TGG 0.371012 7:+41837943 None:intergenic
CCATAAGAATTCCATAATAA+AGG 0.371929 7:+41836309 None:intergenic
AACTCGAGTGTGCAATGTTC+TGG 0.374857 7:-41835515 MsaT036703.1:CDS
AACTCAACAATCCTGATCTC+AGG 0.384176 7:-41837890 MsaT036703.1:CDS
TTGTGCTGTGGTGCTAGATT+GGG 0.395336 7:+41837944 None:intergenic
CTTGCTAGTCTTGGAGTCAT+TGG 0.399485 7:-41834829 MsaT036703.1:CDS
CATTTAAGGCTTGCTAGTCT+TGG 0.411996 7:-41834838 MsaT036703.1:CDS
AAGCCTTAAATGTTCAAATT+GGG 0.413189 7:+41834849 None:intergenic
ACACATTTGGACAAATTGCT+TGG 0.414892 7:-41827672 MsaT036703.1:CDS
TCTGATATATCCAACACATT+TGG 0.418453 7:-41827685 MsaT036703.1:CDS
CACTGGTAAGGCAGTGATGC+TGG 0.420314 7:-41831727 MsaT036703.1:CDS
GAAGTAGGACTTGGCCCTGC+TGG 0.426559 7:-41831213 MsaT036703.1:CDS
GATTGGGATGAAGCAGTTAG+AGG 0.438610 7:+41837960 None:intergenic
TCGCTTGTCGTTCGAAGGAA+TGG 0.440010 7:+41834877 None:intergenic
GAAGTTCTTCCATTAAAGAA+AGG 0.443893 7:+41831696 None:intergenic
TTCAATAACAAGGCGTTCCA+TGG 0.458343 7:+41837911 None:intergenic
TGCCTACGCAGCATGGAAAT+CGG 0.460606 7:-41834094 MsaT036703.1:CDS
AAGGAAATCTATGCTTTCCT+TGG 0.460812 7:+41834141 None:intergenic
AGCAATTTGTCCAAATGTGT+TGG 0.481139 7:+41827675 None:intergenic
AAAATTATGTCAGATGATGA+TGG 0.481246 7:-41833881 MsaT036703.1:CDS
TGCTCTTCGTGTTCTCTCCA+AGG 0.482455 7:-41837862 MsaT036703.1:intron
GTTTCTCGCGTGATCCTGCT+GGG 0.485040 7:-41827590 MsaT036703.1:CDS
AGATTCAACAGCGGAATAGT+AGG 0.487502 7:+41835553 None:intergenic
ATGTTTGCAGGTCACTGGTA+AGG 0.491200 7:-41831739 MsaT036703.1:intron
GATGGTTTGATTTATGTTTG+TGG 0.502440 7:-41833863 MsaT036703.1:CDS
AGCACAAAGCATGGCATCCA+TGG 0.505480 7:-41837928 MsaT036703.1:CDS
GGATATCTAAGTTACCCAGC+AGG 0.522470 7:+41831198 None:intergenic
TTATTATTAGCTAACCTGAA+TGG 0.528931 7:+41831674 None:intergenic
TGTTGATGTTTGCAGGTCAC+TGG 0.529088 7:-41831744 MsaT036703.1:intron
AAGTAGGACTTGGCCCTGCT+GGG 0.529264 7:-41831212 MsaT036703.1:intron
TCTTGGAGTCATTGGTGCAT+TGG 0.530074 7:-41834821 MsaT036703.1:CDS
GAATTATCTCACTTCTAAGA+AGG 0.538844 7:+41834122 None:intergenic
ATCAAGGCTCACCTTCATCC+TGG 0.543372 7:-41827558 MsaT036703.1:CDS
TCCACTATGCCTACGCAGCA+TGG 0.546502 7:-41834101 MsaT036703.1:CDS
GGATGCCATGCTTTGTGCTG+TGG 0.548520 7:+41837932 None:intergenic
CTAACTCGCTTGTCGTTCGA+AGG 0.550213 7:+41834872 None:intergenic
GTGGTTGTGGAAGTTGTCTA+TGG 0.552553 7:-41827903 MsaT036703.1:CDS
ATTATGGTCCACCAGGATGA+AGG 0.555644 7:+41827547 None:intergenic
CTACTTATAGACATTGAAGT+AGG 0.559028 7:-41831228 MsaT036703.1:CDS
TGCTGGGATTGAGAAAATCA+AGG 0.559403 7:-41827574 MsaT036703.1:CDS
ACTCAACAATCCTGATCTCA+GGG 0.561365 7:-41837889 MsaT036703.1:CDS
TCCGCTGTTGAATCTTACTC+AGG 0.564161 7:-41835545 MsaT036703.1:CDS
TCCATGCTGCGTAGGCATAG+TGG 0.580220 7:+41834100 None:intergenic
ATAGACATTGAAGTAGGACT+TGG 0.586139 7:-41831222 MsaT036703.1:CDS
AAACAACGATACCTCTTGCA+TGG 0.595813 7:+41827800 None:intergenic
TGCCGATTTCCATGCTGCGT+AGG 0.604124 7:+41834092 None:intergenic
TAGCACCACAGCACAAAGCA+TGG 0.612717 7:-41837937 MsaT036703.1:CDS
AGTCATTGGTGCATTGGTGA+AGG 0.613240 7:-41834815 MsaT036703.1:intron
GATATCTAAGTTACCCAGCA+GGG 0.617193 7:+41831199 None:intergenic
CACAAGAAGTACTTACGCGA+AGG 0.618083 7:+41833554 None:intergenic
GATTTGGATTATGGTCCACC+AGG 0.621893 7:+41827540 None:intergenic
AAAATACAAATTCAGCATTG+TGG 0.627275 7:+41827877 None:intergenic
AGTTTGATCAATTGATGTGA+AGG 0.628164 7:+41833773 None:intergenic
AACATGGTATTGGAAAGTGT+TGG 0.637520 7:-41833803 MsaT036703.1:CDS
GTTGTGGAAGTTGTCTATGG+TGG 0.638748 7:-41827900 MsaT036703.1:CDS
AAGGCTCACCTTCATCCTGG+TGG 0.639182 7:-41827555 MsaT036703.1:CDS
AAATGTGTTGGATATATCAG+AGG 0.642160 7:+41827687 None:intergenic
GATTGTTGAGTTCAATAACA+AGG 0.670356 7:+41837901 None:intergenic
TCTTAGGAAGACCATGCAAG+AGG 0.688256 7:-41827811 MsaT036703.1:intron
TGAGACAAAAGAGAATAACA+TGG 0.703854 7:+41833743 None:intergenic
ATAGTCACAGGCTAACACCA+AGG 0.732486 7:-41834158 MsaT036703.1:intron
GCCTGAGTAAGATTCAACAG+CGG 0.773865 7:+41835544 None:intergenic
ACTGATGATTGATGATAACG+CGG 0.786782 7:+41827618 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 41827548 41838009 41827548 ID=MsaG036703
Chr7 mRNA 41827548 41838009 41827548 ID=MsaT036703.1;Parent=MsaG036703
Chr7 exon 41827548 41827724 41827548 ID=MsaT036703.1.exon12;Parent=MsaT036703.1
Chr7 CDS 41827548 41827724 41827548 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41827812 41827932 41827812 ID=MsaT036703.1.exon11;Parent=MsaT036703.1
Chr7 CDS 41827812 41827932 41827812 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41831213 41831310 41831213 ID=MsaT036703.1.exon10;Parent=MsaT036703.1
Chr7 CDS 41831213 41831310 41831213 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41831689 41831751 41831689 ID=MsaT036703.1.exon9;Parent=MsaT036703.1
Chr7 CDS 41831689 41831751 41831689 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41833552 41833639 41833552 ID=MsaT036703.1.exon8;Parent=MsaT036703.1
Chr7 CDS 41833552 41833639 41833552 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41833734 41833924 41833734 ID=MsaT036703.1.exon7;Parent=MsaT036703.1
Chr7 CDS 41833734 41833924 41833734 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41834075 41834170 41834075 ID=MsaT036703.1.exon6;Parent=MsaT036703.1
Chr7 CDS 41834075 41834170 41834075 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41834816 41834922 41834816 ID=MsaT036703.1.exon5;Parent=MsaT036703.1
Chr7 CDS 41834816 41834922 41834816 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41835271 41835313 41835271 ID=MsaT036703.1.exon4;Parent=MsaT036703.1
Chr7 CDS 41835271 41835313 41835271 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41835504 41835593 41835504 ID=MsaT036703.1.exon3;Parent=MsaT036703.1
Chr7 CDS 41835504 41835593 41835504 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41836287 41836364 41836287 ID=MsaT036703.1.exon2;Parent=MsaT036703.1
Chr7 CDS 41836287 41836364 41836287 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Chr7 exon 41837863 41838009 41837863 ID=MsaT036703.1.exon1;Parent=MsaT036703.1
Chr7 CDS 41837863 41838009 41837863 ID=cds.MsaT036703.1;Parent=MsaT036703.1
Gene Sequence

>MsaT036703.1

ATGAATAACGTTGCGTCGTTTGTAAGTCCTCTAACTGCTTCATCCCAATCTAGCACCACAGCACAAAGCATGGCATCCATGGAACGCCTTGTTATTGAACTCAACAATCCTGATCTCAGGGAAAATGCTCTTCGTGTTCTCTCCAAGTTTCGGAGGATTGGGTTATTTCCAGAACTTGCACCTTTATTATGGAATTCTTATGGTACTATTGAAATATTTTTACAGGAAATAACTTCAATATATCCTACTATTCCGCTGTTGAATCTTACTCAGGCACAATCAACTCGAGTGTGCAATGTTCTGGAACTACTTCAGTGTGTGGCATCTCACCCTGATACAAAAATGTCATTTCTCAATGCTAACATGCCTTTATATTTTTATCCATTCCTTCGAACGACAAGCGAGTTAGCCCAATTTGAACATTTAAGGCTTGCTAGTCTTGGAGTCATTGGTGCATTGGTGAAGGCTAACACCAAGGAAAGCATAGATTTCCTTCTTAGAAGTGAGATAATTCCACTATGCCTACGCAGCATGGAAATCGGCAAAGAATTATCCAAAACTGTTGCAACATTTATAATTCAAAAAATTATGTCAGATGATGATGGTTTGATTTATGTTTGTGGAACAGCAGAACGTTTTTTTGCAGTAGTTCAAGTTTTGAACATGGTATTGGAAAGTGTTGGCAATCAGCCTTCACATCAATTGATCAAACTTCTGATTCCATGTTATTCTCTTTTGTCTCAACATCCTAGGGCTTGCAATGCATTAAAAAGGCGACTTCCAAACATGCTGAAAACTGTCAATTTCATCAATTGCCTTCGCGTAAGTACTTCTTGTGATGTCACTGGTAAGGCAGTGATGCTGGATAATAAACCTTTCTTTAATGGAAGAACTTCCATTCAGATTTATAGCGACTACATGAAGAGTTTTAGAGAGAACATGGCTGATTTCTTAGAATCTGAGCTACTTATAGACATTGAAGTAGGACTTGGCCCTGCTGGATACAAAGTGGTTGTGGAAGTTGTCTATGGTGGCCACAATGCTGAATTTGTATTTTGGGATAGGGAATGTCTTCAGATTATTGGCATTACTGCTGATGCTCTTAGGAAGACCATGCAAGAGGTCGGTGAAGATGATCCTCTGATATATCCAACACATTTGGACAAATTGCTTGGACTTGAGTTTGCGTTTCGTGCAAAGTATCAGCCGCGTTATCATCAATCATCAGTTCTAGGTTTCTCGCGTGATCCTGCTGGGATTGAGAAAATCAAGGCTCACCTTCATCCTGGTGGACCATAA

Protein sequence

>MsaT036703.1

MNNVASFVSPLTASSQSSTTAQSMASMERLVIELNNPDLRENALRVLSKFRRIGLFPELAPLLWNSYGTIEIFLQEITSIYPTIPLLNLTQAQSTRVCNVLELLQCVASHPDTKMSFLNANMPLYFYPFLRTTSELAQFEHLRLASLGVIGALVKANTKESIDFLLRSEIIPLCLRSMEIGKELSKTVATFIIQKIMSDDDGLIYVCGTAERFFAVVQVLNMVLESVGNQPSHQLIKLLIPCYSLLSQHPRACNALKRRLPNMLKTVNFINCLRVSTSCDVTGKAVMLDNKPFFNGRTSIQIYSDYMKSFRENMADFLESELLIDIEVGLGPAGYKVVVEVVYGGHNAEFVFWDRECLQIIGITADALRKTMQEVGEDDPLIYPTHLDKLLGLEFAFRAKYQPRYHQSSVLGFSRDPAGIEKIKAHLHPGGP*