AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036705

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036705 MtrunA17_Chr1g0154241 94.144 222 13 0 4 225 36 257 7.36e-153 425
MsaG036705 MtrunA17_Chr1g0154101 59.176 267 62 4 4 224 197 462 5.15e-92 287
MsaG036705 MtrunA17_Chr1g0154101 74.194 124 32 0 4 127 40 163 1.05e-57 195
MsaG036705 MtrunA17_Chr1g0154211 63.393 224 73 3 4 224 37 254 4.81e-91 273
MsaG036705 MtrunA17_Chr1g0154251 59.471 227 78 5 4 217 41 266 1.66e-80 249
MsaG036705 MtrunA17_Chr1g0154111 60.811 222 58 4 4 224 41 234 3.14e-78 246
MsaG036705 MtrunA17_Chr1g0154111 56.140 228 71 8 4 225 368 572 4.30e-66 214
MsaG036705 MtrunA17_Chr1g0154191 59.111 225 49 5 4 224 37 222 2.12e-75 232
MsaG036705 MtrunA17_Chr1g0154151 54.185 227 57 5 1 224 16 198 1.04e-68 214
MsaG036705 MtrunA17_Chr1g0154171 55.357 224 59 5 4 224 37 222 6.94e-64 202
MsaG036705 MtrunA17_Chr1g0154131 71.200 125 36 0 4 128 40 164 1.62e-56 186
MsaG036705 MtrunA17_Chr1g0154131 52.451 204 45 4 4 206 197 349 1.75e-56 186
MsaG036705 MtrunA17_Chr1g0154161 76.190 105 24 1 4 108 45 148 4.98e-49 163
MsaG036705 MtrunA17_Chr1g0154071 66.942 121 40 0 4 124 170 290 1.85e-48 166
MsaG036705 MtrunA17_Chr1g0154071 73.267 101 27 0 4 104 42 142 3.51e-47 162
MsaG036705 MtrunA17_Chr1g0154041 36.047 86 54 1 5 90 31 115 8.46e-13 67.0
MsaG036705 MtrunA17_Chr7g0233561 36.486 74 47 0 5 78 34 107 4.50e-11 61.6
MsaG036705 MtrunA17_Chr3g0131891 36.709 79 50 0 5 83 35 113 8.95e-11 60.5
MsaG036705 MtrunA17_Chr7g0233531 36.709 79 50 0 5 83 2 80 9.80e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036705 AT4G33280.2 55.208 96 42 1 4 98 38 133 3.62e-29 110
MsaG036705 AT4G33280.1 55.208 96 42 1 4 98 38 133 1.11e-28 110
MsaG036705 AT4G33280.3 55.208 96 42 1 4 98 38 133 1.55e-28 111
MsaG036705 AT4G34400.2 36.145 83 53 0 4 86 30 112 1.43e-14 72.4
MsaG036705 AT4G34400.1 36.145 83 53 0 4 86 30 112 1.43e-14 72.4
MsaG036705 AT3G06220.1 27.273 110 72 2 5 114 25 126 6.07e-12 62.4
MsaG036705 AT3G18990.2 37.333 75 47 0 5 79 22 96 7.86e-12 64.3
MsaG036705 AT5G60130.5 38.636 88 49 2 4 86 28 115 7.96e-12 63.2
MsaG036705 AT3G18990.1 37.333 75 47 0 5 79 22 96 1.01e-11 63.9
MsaG036705 AT5G60130.3 38.636 88 49 2 4 86 2 89 1.19e-11 63.2
MsaG036705 AT5G60130.4 38.636 88 49 2 4 86 25 112 1.55e-11 63.2
MsaG036705 AT5G60130.1 38.636 88 49 2 4 86 28 115 1.58e-11 63.2
MsaG036705 AT5G60130.2 38.636 88 49 2 4 86 28 115 1.83e-11 62.8

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTCCACTAGGACATTTA+AGG 0.197699 7:+41916831 None:intergenic
GCTGGTCTTCCCCGCTTCTT+AGG 0.270434 7:+41916311 None:intergenic
CCCCGCTTCTTAGGCTGGCC+AGG 0.320088 7:+41916320 None:intergenic
CCAGGGGTAACTTCGTTAAC+AGG 0.339699 7:+41916338 None:intergenic
GTCTTCCCCGCTTCTTAGGC+TGG 0.353235 7:+41916315 None:intergenic
AACTTCGTTAACAGGCTTCT+TGG 0.357459 7:+41916346 None:intergenic
CCAGCAAAATCAGCTACTTC+TGG 0.385623 7:-41916293 MsaT036705.1:CDS
CCCGCTTCTTAGGCTGGCCA+GGG 0.386604 7:+41916321 None:intergenic
GTGGAGTTGTGTGGAATATA+TGG 0.388254 7:-41916817 MsaT036705.1:CDS
AATGTGGTTCTGAGAAATTT+CGG 0.410547 7:-41916520 MsaT036705.1:CDS
GCATCAGCCATGACCTGCTC+AGG 0.424246 7:+41916410 None:intergenic
TTAGTTAGAAAATGTGGAAA+AGG 0.447930 7:-41916626 MsaT036705.1:CDS
TGAGTCTGCTTCCCCTGAGC+AGG 0.449413 7:-41916423 MsaT036705.1:CDS
GCGAGAAAGCAATTGTTGAT+TGG 0.450466 7:-41916271 MsaT036705.1:CDS
CCCTGGCCAGCCTAAGAAGC+GGG 0.452831 7:-41916321 MsaT036705.1:CDS
AACTCCACTAGGACATTTAA+GGG 0.453248 7:+41916832 None:intergenic
TGCTTCCCCTGAGCAGGTCA+TGG 0.458901 7:-41916417 MsaT036705.1:CDS
GAAGTCAACACTGCTTCTAA+TGG 0.464985 7:-41916551 MsaT036705.1:CDS
AGAGCCCGCTTTCTCGCATA+AGG 0.473371 7:+41916652 None:intergenic
GACACTGTTTACTTCACAAA+CGG 0.478703 7:-41916779 MsaT036705.1:CDS
CGAGAAAGCAATTGTTGATT+GGG 0.485091 7:-41916270 MsaT036705.1:CDS
GCGGTTCCCTCCCAAACAAC+CGG 0.485847 7:-41916377 MsaT036705.1:CDS
TACTTCACAAACGGTTGGCA+AGG 0.517034 7:-41916770 MsaT036705.1:CDS
CTGTTTACTTCACAAACGGT+TGG 0.547920 7:-41916775 MsaT036705.1:CDS
AATGAAAAGACTTATAATGC+TGG 0.548214 7:-41916449 MsaT036705.1:CDS
CATCAGCCATGACCTGCTCA+GGG 0.563227 7:+41916411 None:intergenic
GAAAGCAATTGTTGATTGGG+TGG 0.568458 7:-41916267 MsaT036705.1:CDS
CCTGTTAACGAAGTTACCCC+TGG 0.570624 7:-41916338 MsaT036705.1:CDS
TCTTACTTAGTTAGAAAATG+TGG 0.573126 7:-41916632 MsaT036705.1:CDS
CCCCTGGCCAGCCTAAGAAG+CGG 0.578924 7:-41916322 MsaT036705.1:CDS
TATATGGCTGACAACTAAAG+AGG 0.582468 7:-41916801 MsaT036705.1:CDS
GAGACCTTATGCGAGAAAGC+GGG 0.590946 7:-41916656 MsaT036705.1:CDS
ATATTCCACACAACTCCACT+AGG 0.593357 7:+41916821 None:intergenic
GGCTGATGCAGTCACTATTG+CGG 0.596142 7:-41916396 MsaT036705.1:CDS
TACTGAGCAAGAGGGCGACA+AGG 0.599125 7:-41916600 MsaT036705.1:CDS
AAAAGGTCATACTGAGCAAG+AGG 0.615156 7:-41916609 MsaT036705.1:CDS
TCTAATGGCGGTGTTGAATG+TGG 0.619297 7:-41916536 MsaT036705.1:CDS
GTGACCCTTAAATGTCCTAG+TGG 0.621044 7:-41916836 MsaT036705.1:CDS
CCGCTTCTTAGGCTGGCCAG+GGG 0.627170 7:+41916322 None:intergenic
TGAGACCTTATGCGAGAAAG+CGG 0.630542 7:-41916657 MsaT036705.1:CDS
GCTTGTAGCAAGGAAAACTC+AGG 0.631925 7:-41916245 MsaT036705.1:CDS
CCTGGCCAGCCTAAGAAGCG+GGG 0.643193 7:-41916320 MsaT036705.1:CDS
AATGTCCTAGTGGAGTTGTG+TGG 0.645544 7:-41916826 MsaT036705.1:CDS
GTCAACACTGCTTCTAATGG+CGG 0.645750 7:-41916548 MsaT036705.1:CDS
TGATTGGGTGGCTTGTAGCA+AGG 0.654638 7:-41916255 MsaT036705.1:CDS
AAAGGTCATACTGAGCAAGA+GGG 0.680883 7:-41916608 MsaT036705.1:CDS
ATCAGCCATGACCTGCTCAG+GGG 0.726483 7:+41916412 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 41916241 41916972 41916241 ID=MsaG036705
Chr7 mRNA 41916241 41916972 41916241 ID=MsaT036705.1;Parent=MsaG036705
Chr7 exon 41916241 41916909 41916241 ID=MsaT036705.1.exon2;Parent=MsaT036705.1
Chr7 CDS 41916241 41916909 41916241 ID=cds.MsaT036705.1;Parent=MsaT036705.1
Chr7 exon 41916964 41916972 41916964 ID=MsaT036705.1.exon1;Parent=MsaT036705.1
Chr7 CDS 41916964 41916972 41916964 ID=cds.MsaT036705.1;Parent=MsaT036705.1
Gene Sequence

>MsaT036705.1

ATGACTAGTGCACTTCCTAAAACATTTTCAGCCAATTTAAAGAAAAAGTTGCCTGAAAATGTGACCCTTAAATGTCCTAGTGGAGTTGTGTGGAATATATGGCTGACAACTAAAGAGGACACTGTTTACTTCACAAACGGTTGGCAAGGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATCTCCTTTTTTTCAAGTACAATGGCGAATCGTTGTTTGAAGTTTTAATATTTCATGGTGAGACCTTATGCGAGAAAGCGGGCTCTTACTTAGTTAGAAAATGTGGAAAAGGTCATACTGAGCAAGAGGGCGACAAGGCAAAAGTTGCAAAGAATTCTGTTGAAGAAGTCAACACTGCTTCTAATGGCGGTGTTGAATGTGGTTCTGAGAAATTTCGGAAGCTTGACACTATAAGAACACCATTAGCTGTACCATTCGAAAATACCAATGAAAAGACTTATAATGCTGGTGTTGAGTCTGCTTCCCCTGAGCAGGTCATGGCTGATGCAGTCACTATTGCGGTTCCCTCCCAAACAACCGGCAAAAAGACCAAGAAGCCTGTTAACGAAGTTACCCCTGGCCAGCCTAAGAAGCGGGGAAGACCAGCAAAATCAGCTACTTCTGGCGAGAAAGCAATTGTTGATTGGGTGGCTTGTAGCAAGGAAAACTCAGGTTAA
Protein sequence

>MsaT036705.1

MTSALPKTFSANLKKKLPENVTLKCPSGVVWNIWLTTKEDTVYFTNGWQGFVNDHSLKESDLLFFKYNGESLFEVLIFHGETLCEKAGSYLVRKCGKGHTEQEGDKAKVAKNSVEEVNTASNGGVECGSEKFRKLDTIRTPLAVPFENTNEKTYNAGVESASPEQVMADAVTIAVPSQTTGKKTKKPVNEVTPGQPKKRGRPAKSATSGEKAIVDWVACSKENSG*