AlfalfaGEDB | MsaG036706

M. sativa ssp. caerulea / MsaG036706

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG036706	XP_024636626.1	60.309	194	59	6	204	380	29	221	2.22e-58	201
MsaG036706	XP_024636626.1	87.619	210	15	1	4	202	36	245	2.45e-124	370

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG036706	sp\|Q8RYD1\|REM16_ARATH	45.000	100	41	3	4	91	38	135	1.51e-16	83.2
MsaG036706	sp\|Q8RYD1\|REM16_ARATH	50.649	77	37	1	210	285	37	113	1.97e-15	80.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG036706	tr\|A0A396JGK5\|A0A396JGK5_MEDTR	60.309	194	59	6	204	380	29	221	1.06e-58	201
MsaG036706	tr\|A0A396JGK5\|A0A396JGK5_MEDTR	87.619	210	15	1	4	202	36	245	1.17e-124	370

TFs/TRs:

Gene ID	Type	Classification
MsaG036706	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000325	MsaG036706	0.804452	3.621395e-49	5.420801e-47
MsaG004280	MsaG036706	0.816453	9.525667e-52	1.910567e-49
MsaG004819	MsaG036706	0.811902	9.527912e-51	1.704596e-48
MsaG004924	MsaG036706	0.802672	8.438761e-49	1.212291e-46
MsaG010706	MsaG036706	0.813971	3.370792e-51	6.349702e-49
MsaG016419	MsaG036706	0.810089	2.343746e-50	4.011099e-48
MsaG016695	MsaG036706	0.813062	5.328511e-51	9.811754e-49
MsaG018054	MsaG036706	0.800496	2.345670e-48	3.207190e-46
MsaG020240	MsaG036706	0.819959	1.546076e-52	3.396816e-50
MsaG023298	MsaG036706	0.823349	2.565808e-53	6.170979e-51
MsaG024134	MsaG036706	0.838450	5.252654e-57	1.953500e-54
MsaG024480	MsaG036706	0.813857	3.571021e-51	6.707751e-49
MsaG026480	MsaG036706	0.810538	1.877336e-50	3.248296e-48
MsaG027572	MsaG036706	0.823282	2.660019e-53	6.386018e-51
MsaG027828	MsaG036706	0.827247	3.102107e-54	8.305634e-52
MsaG028661	MsaG036706	0.806450	1.386468e-49	2.174766e-47
MsaG029091	MsaG036706	0.801181	1.702705e-48	2.364329e-46
MsaG029147	MsaG036706	0.832527	1.625473e-55	5.060806e-53
MsaG029561	MsaG036706	0.809861	2.622364e-50	4.463268e-48
MsaG029546	MsaG036706	0.800157	2.747765e-48	3.728245e-46
MsaG033781	MsaG036706	0.816063	1.163114e-51	2.309766e-49
MsaG036706	MsaG040728	0.807294	9.208845e-50	1.473466e-47
MsaG036706	MsaG047097	0.810706	1.726938e-50	3.000297e-48
MsaG036706	MsaG002243	0.814861	2.147389e-51	4.136577e-49
MsaG036706	MsaG013496	0.808442	5.263856e-50	8.657818e-48
MsaG036706	MsaG028079	0.802805	7.921348e-49	1.141423e-46
MsaG036706	MsaG030561	0.819023	2.522315e-52	5.406553e-50
MsaG036706	MsaG036286	0.818823	2.799515e-52	5.969204e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG036706	MtrunA17_Chr1g0154241	87.619	210	15	1	4	202	36	245	2.26e-128	370
MsaG036706	MtrunA17_Chr1g0154241	60.309	194	59	6	204	380	29	221	2.04e-62	201
MsaG036706	MtrunA17_Chr1g0154101	43.636	385	125	6	4	359	40	361	7.61e-85	275
MsaG036706	MtrunA17_Chr1g0154101	41.294	402	115	7	4	284	197	598	5.82e-75	248
MsaG036706	MtrunA17_Chr1g0154101	77.108	83	19	0	204	286	33	115	3.35e-36	140
MsaG036706	MtrunA17_Chr1g0154211	61.033	213	64	3	4	202	37	244	6.67e-79	248
MsaG036706	MtrunA17_Chr1g0154211	48.824	170	68	2	204	355	30	198	4.67e-41	149
MsaG036706	MtrunA17_Chr1g0154131	47.440	293	82	4	4	285	40	271	8.76e-76	242
MsaG036706	MtrunA17_Chr1g0154131	40.705	312	88	12	4	272	197	454	5.67e-50	174
MsaG036706	MtrunA17_Chr1g0154131	72.619	84	23	0	203	286	32	115	3.51e-34	132
MsaG036706	MtrunA17_Chr1g0154251	39.642	391	169	12	4	334	41	424	1.49e-69	227
MsaG036706	MtrunA17_Chr1g0154111	45.092	326	100	8	4	272	41	344	2.68e-68	226
MsaG036706	MtrunA17_Chr1g0154111	58.537	205	68	6	4	202	368	561	1.91e-63	213
MsaG036706	MtrunA17_Chr1g0154111	65.359	153	45	3	211	362	368	513	6.96e-54	187
MsaG036706	MtrunA17_Chr1g0154111	50.602	166	68	3	204	355	34	199	9.55e-39	146
MsaG036706	MtrunA17_Chr1g0154191	41.834	349	102	10	4	284	37	352	1.72e-62	204
MsaG036706	MtrunA17_Chr1g0154191	55.696	158	40	4	211	362	37	170	3.88e-41	149
MsaG036706	MtrunA17_Chr1g0154151	41.159	328	93	8	1	272	16	299	8.09e-61	199
MsaG036706	MtrunA17_Chr1g0154151	48.951	143	60	4	211	346	19	155	8.59e-32	122
MsaG036706	MtrunA17_Chr1g0154171	39.884	346	104	11	4	285	37	342	2.88e-55	186
MsaG036706	MtrunA17_Chr1g0154171	52.632	152	62	3	205	346	31	182	2.27e-37	138
MsaG036706	MtrunA17_Chr1g0154161	37.838	296	136	9	4	285	45	306	4.81e-41	147
MsaG036706	MtrunA17_Chr1g0154161	83.333	78	13	0	211	288	45	122	1.28e-37	138
MsaG036706	MtrunA17_Chr1g0154071	36.970	330	148	12	4	287	170	485	4.83e-40	148
MsaG036706	MtrunA17_Chr1g0154071	65.347	101	24	1	4	93	42	142	2.88e-35	135
MsaG036706	MtrunA17_Chr1g0154071	72.941	85	23	0	204	288	35	119	4.04e-33	129
MsaG036706	MtrunA17_Chr1g0154071	74.026	77	20	0	209	285	168	244	1.93e-30	121
MsaG036706	MtrunA17_Chr7g0233561	25.329	304	182	12	5	287	34	313	2.54e-13	70.5
MsaG036706	MtrunA17_Chr1g0154041	36.047	86	55	0	203	288	22	107	4.59e-12	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG036706	AT4G33280.2	44.792	96	41	2	4	87	38	133	6.63e-18	83.6
MsaG036706	AT4G33280.2	50.649	77	37	1	210	285	37	113	1.34e-16	79.7
MsaG036706	AT4G33280.1	45.000	100	41	3	4	91	38	135	1.53e-17	83.2
MsaG036706	AT4G33280.1	50.649	77	37	1	210	285	37	113	2.00e-16	80.1
MsaG036706	AT4G33280.3	45.000	100	41	3	4	91	38	135	1.79e-17	84.0
MsaG036706	AT4G33280.3	50.649	77	37	1	210	285	37	113	2.80e-16	80.1
MsaG036706	AT4G34400.2	38.750	80	49	0	206	285	25	104	2.44e-15	77.4
MsaG036706	AT4G34400.2	38.028	71	44	0	4	74	30	100	2.99e-13	71.2
MsaG036706	AT4G34400.1	38.750	80	49	0	206	285	25	104	2.44e-15	77.4
MsaG036706	AT4G34400.1	38.028	71	44	0	4	74	30	100	2.99e-13	71.2
MsaG036706	AT3G06220.1	33.784	74	49	0	212	285	25	98	3.02e-13	67.8
MsaG036706	AT3G06220.1	28.704	108	62	2	5	103	25	126	4.15e-12	64.7
MsaG036706	AT3G06160.1	36.585	82	49	1	204	285	35	113	3.62e-12	67.4
MsaG036706	AT3G06160.1	28.025	157	94	4	4	159	39	177	4.57e-11	63.9
MsaG036706	AT3G06160.5	36.585	82	49	1	204	285	20	98	3.74e-12	67.0
MsaG036706	AT3G06160.5	28.025	157	94	4	4	159	24	162	3.22e-11	64.3
MsaG036706	AT3G06160.4	36.585	82	49	1	204	285	20	98	3.74e-12	67.0
MsaG036706	AT3G06160.4	28.025	157	94	4	4	159	24	162	3.22e-11	64.3
MsaG036706	AT3G06160.3	36.585	82	49	1	204	285	20	98	3.74e-12	67.0
MsaG036706	AT3G06160.3	28.025	157	94	4	4	159	24	162	3.22e-11	64.3
MsaG036706	AT3G06160.2	36.585	82	49	1	204	285	79	157	4.37e-12	67.4
MsaG036706	AT3G06160.2	28.025	157	94	4	4	159	83	221	4.24e-11	64.3
MsaG036706	AT5G66980.1	45.122	82	37	3	210	285	23	102	1.78e-11	65.1

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACTCCACTAGGACCTTTA+AGG	0.172719	7:-41917580	None:intergenic
CTACTCCACTAGGACCTTTA+AGG	0.172719	7:-41939904	None:intergenic
CCTGAAAATGTGACCCTTAA+AGG	0.191053	7:+41917566	MsaT036706.1:CDS
CCTGAAAATGTGACCCTTAA+AGG	0.191053	7:+41939890	MsaT036706.1:CDS
AAGACACAAGAACGCGAAAT+AGG	0.246820	7:-41930002	None:intergenic
TCTGGTCTTCCCCGCTTCTT+AGG	0.272873	7:-41918104	None:intergenic
GTCTTCCCCGCTTCTTAGGC+TGG	0.343652	7:-41918100	None:intergenic
CCAGGCGTAACTTCGTTAAC+AGG	0.352395	7:-41918077	None:intergenic
AATGCGGTTCTGAGAAATTT+CGG	0.363117	7:+41917895	MsaT036706.1:CDS
CGAGAAAGCGTGCTCTTACT+TGG	0.378847	7:+41917770	MsaT036706.1:intron
CCCCGCTTCTTAGGCTGGCC+AGG	0.381562	7:-41918095	None:intergenic
AGACACAAGAACGCGAAATA+GGG	0.418363	7:-41930001	None:intergenic
GCATCAGCCATGACCTGCTC+AGG	0.424246	7:-41918005	None:intergenic
AACTTCGTTAACAGGCTTCT+CGG	0.427121	7:-41918069	None:intergenic
AAGACCGAGAAGCTCGTTAA+CGG	0.428507	7:+41940381	MsaT036706.1:CDS
TTGGTTAGAAAATGTGGAAA+AGG	0.434707	7:+41917789	MsaT036706.1:CDS
TTGGTTAGAAAATGTGGAAA+AGG	0.434707	7:+41940113	MsaT036706.1:intron
TGAGTCTGCTTCCCCTGAGC+AGG	0.449413	7:+41917992	MsaT036706.1:CDS
TGCGATTCCTCCCAAACAAC+CGG	0.452034	7:+41940355	MsaT036706.1:CDS
TGCTTCCCCTGAGCAGGTCA+TGG	0.458901	7:+41917998	MsaT036706.1:CDS
GAAGTCAACACTGCTTCTAA+TGG	0.464985	7:+41917864	MsaT036706.1:CDS
GAAGTCAACACTGCTTCTAA+TGG	0.464985	7:+41940188	MsaT036706.1:CDS
GCCTGGCCAGCCTAAGAAGC+GGG	0.466869	7:+41918094	MsaT036706.1:CDS
GCGGTTCCCTCCCAAACAAC+TGG	0.474593	7:+41918038	MsaT036706.1:CDS
TACTCCACTAGGACCTTTAA+GGG	0.477835	7:-41917579	None:intergenic
TACTCCACTAGGACCTTTAA+GGG	0.477835	7:-41939903	None:intergenic
GACACTGTTTACTTCACAAA+CGG	0.478703	7:+41917632	MsaT036706.1:CDS
GACACTGTTTACTTCACAAA+CGG	0.478703	7:+41939956	MsaT036706.1:CDS
TACTTCACAAACGGTTGGCA+AGG	0.517034	7:+41917641	MsaT036706.1:CDS
TACTTCACAAACGGTTGGCA+AGG	0.517034	7:+41939965	MsaT036706.1:CDS
GTAACCGTTAACGAGCTTCT+CGG	0.522088	7:-41940385	None:intergenic
TCTTACTTGGTTAGAAAATG+TGG	0.532406	7:+41917783	MsaT036706.1:CDS
TCTTACTTGGTTAGAAAATG+TGG	0.532406	7:+41940107	MsaT036706.1:intron
CCTGTTAACGAAGTTACGCC+TGG	0.535294	7:+41918077	MsaT036706.1:CDS
TATATGCTTGACAACTAAAG+AGG	0.535537	7:+41917610	MsaT036706.1:CDS
TATATGCTTGACAACTAAAG+AGG	0.535537	7:+41939934	MsaT036706.1:CDS
CTGTTTACTTCACAAACGGT+TGG	0.547920	7:+41917636	MsaT036706.1:CDS
CTGTTTACTTCACAAACGGT+TGG	0.547920	7:+41939960	MsaT036706.1:CDS
AATGAAAAGACTTATAATGC+TGG	0.548214	7:+41917966	MsaT036706.1:CDS
AATGAAAAGACTTATAATGC+TGG	0.548214	7:+41940288	MsaT036706.1:CDS
GTCCGCTTCGAGCAGTGTCA+TGG	0.552007	7:+41940317	MsaT036706.1:CDS
TGGTCTTCCCGCTTCTTAGG+TGG	0.555857	7:-41940412	None:intergenic
CATCAGCCATGACCTGCTCA+GGG	0.563227	7:-41918004	None:intergenic
CGCCTGGCCAGCCTAAGAAG+CGG	0.563693	7:+41918093	MsaT036706.1:CDS
GAAATAGGGCATGCTGTCAG+AGG	0.583681	7:-41929987	None:intergenic
AGCCATGACACTGCTCGAAG+CGG	0.586314	7:-41940319	None:intergenic
GGCTGATGCAGTCACTATTG+CGG	0.596142	7:+41918019	MsaT036706.1:CDS
TACTGAGCAAGAGGGAGACA+AGG	0.597506	7:+41917815	MsaT036706.1:CDS
TACTGAGCAAGAGGGAGACA+AGG	0.597506	7:+41940139	MsaT036706.1:CDS
AAAAGGTCATACTGAGCAAG+AGG	0.610370	7:+41917806	MsaT036706.1:CDS
AAAAGGTCATACTGAGCAAG+AGG	0.610370	7:+41940130	MsaT036706.1:CDS
TCTTCCCGCTTCTTAGGTGG+CGG	0.611982	7:-41940409	None:intergenic
AAATAGGGCATGCTGTCAGA+GGG	0.616255	7:-41929986	None:intergenic
ATATTCCACACTACTCCACT+AGG	0.619974	7:-41917590	None:intergenic
ATATTCCACACTACTCCACT+AGG	0.619974	7:-41939914	None:intergenic
GTGACCCTTAAAGGTCCTAG+TGG	0.630968	7:+41917575	MsaT036706.1:CDS
GTGACCCTTAAAGGTCCTAG+TGG	0.630968	7:+41939899	MsaT036706.1:CDS
AAGGTCCTAGTGGAGTAGTG+TGG	0.632095	7:+41917585	MsaT036706.1:CDS
AAGGTCCTAGTGGAGTAGTG+TGG	0.632095	7:+41939909	MsaT036706.1:CDS
CCTGGCCAGCCTAAGAAGCG+GGG	0.640983	7:+41918095	MsaT036706.1:CDS
GCAAGAGGGAGACAAGGCAA+AGG	0.642426	7:+41917821	MsaT036706.1:CDS
GCAAGAGGGAGACAAGGCAA+AGG	0.642426	7:+41940145	MsaT036706.1:CDS
GTCAACACTGCTTCTAATGG+CGG	0.645750	7:+41917867	MsaT036706.1:CDS
GTCAACACTGCTTCTAATGG+CGG	0.645750	7:+41940191	MsaT036706.1:CDS
TCTAATGGCGGTGTTGAATG+CGG	0.661026	7:+41917879	MsaT036706.1:CDS
AAAGGTCATACTGAGCAAGA+GGG	0.680883	7:+41917807	MsaT036706.1:CDS
AAAGGTCATACTGAGCAAGA+GGG	0.680883	7:+41940131	MsaT036706.1:CDS
ATCAGCCATGACCTGCTCAG+GGG	0.726483	7:-41918003	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	41917461	41940419	41917461	ID=MsaG036706
Chr7	mRNA	41917461	41940419	41917461	ID=MsaT036706.1;Parent=MsaG036706
Chr7	exon	41917461	41917469	41917461	ID=MsaT036706.1.exon1;Parent=MsaT036706.1
Chr7	CDS	41917461	41917469	41917461	ID=cds.MsaT036706.1;Parent=MsaT036706.1
Chr7	exon	41917524	41917736	41917524	ID=MsaT036706.1.exon2;Parent=MsaT036706.1
Chr7	CDS	41917524	41917736	41917524	ID=cds.MsaT036706.1;Parent=MsaT036706.1
Chr7	exon	41917774	41918157	41917774	ID=MsaT036706.1.exon3;Parent=MsaT036706.1
Chr7	CDS	41917774	41918157	41917774	ID=cds.MsaT036706.1;Parent=MsaT036706.1
Chr7	exon	41929986	41930009	41929986	ID=MsaT036706.1.exon4;Parent=MsaT036706.1
Chr7	CDS	41929986	41930009	41929986	ID=cds.MsaT036706.1;Parent=MsaT036706.1
Chr7	exon	41939848	41940076	41939848	ID=MsaT036706.1.exon5;Parent=MsaT036706.1
Chr7	CDS	41939848	41940076	41939848	ID=cds.MsaT036706.1;Parent=MsaT036706.1
Chr7	exon	41940121	41940419	41940121	ID=MsaT036706.1.exon6;Parent=MsaT036706.1
Chr7	CDS	41940121	41940419	41940121	ID=cds.MsaT036706.1;Parent=MsaT036706.1

Gene Sequence

>MsaT036706.1

ATGACTAGTGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAAAAGTTGCCTGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTAGTGTGGAATATATGCTTGACAACTAAAGAGGACACTGTTTACTTCACAAACGGTTGGCAAGGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATCTCCTTTTTTTCATGTACAATGGCGAATCATTGTTTGAAAAAGCGTGCTCTTACTTGGTTAGAAAATGTGGAAAAGGTCATACTGAGCAAGAGGGAGACAAGGCAAAGGTTGCAAAGAATTCTGTTGAAGAAGTCAACACTGCTTCTAATGGCGGTGTTGAATGCGGTTCTGAGAAATTTCGGAAGCTTGACACTATAAGAACACCATTAGCTGTACCTTTCGAAAATACCAATGAAAAGACTTATAATGCTGGTGTTGAGTCTGCTTCCCCTGAGCAGGTCATGGCTGATGCAGTCACTATTGCGGTTCCCTCCCAAACAACTGGCAAAAAGACCGAGAAGCCTGTTAACGAAGTTACGCCTGGCCAGCCTAAGAAGCGGGGAAGACCAGAAAAATCAGCTATTTCTGGCGAGAAAGCAATTCCCTCTGACAGCATGCCCTATTTCGCACTTCCTAAAACATTTTCAGACAATTTAAAGAAAAAGTTGCCTGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTAGTGTGGAATATATGCTTGACAACTAAAGAGGACACTGTTTACTTCACAAACGGTTGGCAAGGATTTGTGAATGATCACTCTTTGAAAGAGAGTGATCTCCTTTTTTTCATGTACAATGGCGAATCATTGTTTGAAGTTTTAATATTTCATGAAAATGTGGAAAAGGTCATACTGAGCAAGAGGGAGACAAGGCAAAGGTTGCAAAGAATTCTGTTGAAGAAGTCAACACTGCTTCTAATGGCGGTGTTGAATGCGATTCGAGAAATTTCGAAGCTTGACACTATAAGAACACCATTAGCTGTACCTTTTCAAAATACCAATGAAAAGACTTATAATGCTGGTGTTGAGTCCGCTTCGAGCAGTGTCATGGCTGATGCGTCACTATTGCGATTCCTCCCAAACAACCGGCAAAAGACCGAGAAGCTCGTTAACGGTTACGCCGCCACCTAA

Protein sequence

>MsaT036706.1

MTSALPKTFSDNLKKKLPENVTLKGPSGVVWNICLTTKEDTVYFTNGWQGFVNDHSLKESDLLFFMYNGESLFEKACSYLVRKCGKGHTEQEGDKAKVAKNSVEEVNTASNGGVECGSEKFRKLDTIRTPLAVPFENTNEKTYNAGVESASPEQVMADAVTIAVPSQTTGKKTEKPVNEVTPGQPKKRGRPEKSAISGEKAIPSDSMPYFALPKTFSDNLKKKLPENVTLKGPSGVVWNICLTTKEDTVYFTNGWQGFVNDHSLKESDLLFFMYNGESLFEVLIFHENVEKVILSKRETRQRLQRILLKKSTLLLMAVLNAIREISKLDTIRTPLAVPFQNTNEKTYNAGVESASSSVMADASLLRFLPNNRQKTEKLVNGYAAT*