AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036746

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036746 MtrunA17_Chr7g0231181 97.050 339 7 1 1 336 23 361 0.0 671
MsaG036746 MtrunA17_Chr4g0056091 43.299 291 123 10 47 330 60 315 7.14e-57 187
MsaG036746 MtrunA17_Chr4g0012691 44.784 393 118 16 1 334 24 376 6.98e-54 181
MsaG036746 MtrunA17_Chr4g0061301 39.873 158 52 4 205 321 340 495 2.36e-25 106
MsaG036746 MtrunA17_Chr5g0399261 36.453 203 97 6 38 237 71 244 1.19e-23 98.2
MsaG036746 MtrunA17_Chr1g0182161 42.735 117 52 4 215 317 39 154 1.69e-21 89.7
MsaG036746 MtrunA17_Chr7g0263131 40.816 147 69 9 188 319 6 149 2.27e-21 89.4
MsaG036746 MtrunA17_Chr1g0207451 37.956 137 64 6 200 316 64 199 9.92e-20 87.0
MsaG036746 MtrunA17_Chr4g0012791 31.897 232 86 7 144 313 159 380 1.79e-19 89.0
MsaG036746 MtrunA17_Chr2g0327901 34.694 147 70 5 178 319 4 129 1.47e-18 80.9
MsaG036746 MtrunA17_Chr3g0134711 39.506 162 70 7 185 320 57 216 1.43e-17 80.9
MsaG036746 MtrunA17_Chr7g0263121 40.351 114 56 5 215 317 44 156 1.62e-17 79.0
MsaG036746 MtrunA17_Chr1g0152661 33.155 187 80 7 141 312 37 193 1.20e-16 78.2
MsaG036746 MtrunA17_Chr1g0182181 38.583 127 60 6 215 326 35 158 1.84e-16 75.9
MsaG036746 MtrunA17_Chr5g0425411 41.176 102 40 3 214 295 118 219 5.11e-16 76.6
MsaG036746 MtrunA17_Chr7g0222831 40.367 109 53 4 181 283 6 108 1.15e-15 74.3
MsaG036746 MtrunA17_Chr4g0027991 33.735 166 55 4 215 327 113 276 1.37e-15 76.3
MsaG036746 MtrunA17_Chr4g0018761 26.752 314 144 12 1 313 26 254 1.00e-14 73.2
MsaG036746 MtrunA17_Chr1g0212561 42.683 82 38 2 207 283 41 118 1.34e-13 68.9
MsaG036746 MtrunA17_Chr4g0043071 50.820 61 22 2 261 316 441 498 1.59e-13 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036746 AT3G60580.1 43.636 330 112 11 1 322 23 286 4.46e-59 192
MsaG036746 AT2G45120.1 43.373 332 134 11 1 322 23 310 1.44e-50 171
MsaG036746 AT1G02030.1 40.426 282 107 10 57 320 25 263 2.17e-34 127
MsaG036746 AT3G53600.1 39.535 129 65 4 215 333 49 174 3.68e-22 92.0
MsaG036746 AT3G19580.1 31.019 216 79 7 140 319 64 245 1.97e-21 92.4
MsaG036746 AT3G19580.2 31.019 216 79 7 140 319 64 245 1.97e-21 92.4
MsaG036746 AT2G37430.1 43.443 122 43 6 214 317 46 159 4.07e-19 84.0
MsaG036746 AT2G28200.1 34.839 155 44 6 215 317 115 264 8.97e-18 82.8
MsaG036746 AT5G61470.1 46.667 75 40 0 211 285 224 298 1.09e-17 82.8
MsaG036746 AT5G43170.1 35.333 150 51 3 142 291 45 148 2.63e-17 79.3
MsaG036746 AT4G35280.1 36.719 128 57 4 214 327 161 278 4.51e-17 80.5
MsaG036746 AT2G28710.1 38.519 135 60 7 200 319 21 147 1.27e-16 76.6
MsaG036746 AT1G49900.1 47.887 71 35 1 215 283 750 820 1.80e-16 80.9
MsaG036746 AT1G49900.1 44.000 75 36 1 215 283 193 267 7.28e-14 73.2
MsaG036746 AT5G03510.1 35.664 143 50 4 213 313 116 258 4.95e-16 77.8
MsaG036746 AT2G17180.1 38.017 121 48 3 214 317 147 257 1.67e-15 75.9
MsaG036746 AT3G49930.1 41.228 114 51 3 215 319 94 200 1.86e-15 74.7
MsaG036746 AT5G67450.1 40.217 92 30 2 215 283 97 186 2.95e-15 74.7
MsaG036746 AT1G27730.1 33.654 208 63 9 140 319 48 208 3.19e-15 74.3
MsaG036746 AT3G46090.1 41.739 115 42 6 215 310 40 148 3.62e-15 72.8
MsaG036746 AT5G59820.1 53.623 69 29 2 215 283 39 104 4.64e-15 72.4
MsaG036746 AT3G46080.1 47.368 76 28 3 215 283 37 107 1.52e-14 70.9
MsaG036746 AT1G26610.1 57.377 61 21 2 259 316 397 455 2.22e-14 74.3
MsaG036746 AT5G04340.1 41.176 85 31 2 215 283 89 170 9.27e-14 70.5
MsaG036746 AT3G46070.1 42.308 78 40 2 211 283 31 108 4.19e-13 67.0
MsaG036746 AT4G16610.1 38.462 117 57 3 43 157 52 155 1.58e-12 66.2

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAAAGGGTCTTAATTA+TGG 0.258113 7:+43351515 MsaT036746.1:CDS
ACGAGACGAATGCTTCTCTT+TGG 0.315457 7:-43351551 None:intergenic
AATTGAAATCGAAAGATCTT+TGG 0.317289 7:+43351940 MsaT036746.1:CDS
CTAACTTCATCATCTTCAAT+TGG 0.374158 7:-43352325 None:intergenic
AAAAGAACACATGTGATTCA+TGG 0.392470 7:+43352226 MsaT036746.1:CDS
AGATGATGACGTTGATGAAA+AGG 0.399134 7:+43351502 MsaT036746.1:CDS
ACAAAATGCAACATCTTCTT+CGG 0.417782 7:-43351816 None:intergenic
TTCAAATCTTATCAAGCATT+GGG 0.429788 7:+43352058 MsaT036746.1:CDS
GCATCAGAAACCGAACTAAC+AGG 0.435004 7:-43351788 None:intergenic
GTTCAAATCTTATCAAGCAT+TGG 0.444666 7:+43352057 MsaT036746.1:CDS
CTTCTTGCTTTGTAACAAGA+AGG 0.459791 7:-43351409 None:intergenic
TGAACATGAACCTGTTAGTT+CGG 0.475159 7:+43351778 MsaT036746.1:CDS
GATTCTTCCACCGTTATGTT+CGG 0.482402 7:-43352112 None:intergenic
GATGATGACGTTGATGAAAA+GGG 0.491203 7:+43351503 MsaT036746.1:CDS
TTCAATTCGGGTCAAGCACT+TGG 0.496045 7:+43352196 MsaT036746.1:CDS
TGCAGTTGTCGAGAGACAAA+TGG 0.497909 7:+43351843 MsaT036746.1:CDS
TCATCATCTTCAATTGGAGC+AGG 0.500559 7:-43352319 None:intergenic
TTCTATATCTTGAAGAATCA+CGG 0.508062 7:-43351612 None:intergenic
AAAGAAAGTTCATGAATGTC+CGG 0.517315 7:+43352159 MsaT036746.1:CDS
AATAATAATAAAGTACGTAA+AGG 0.519533 7:+43352004 MsaT036746.1:CDS
CAACTCGGAAAAGATCCAAA+AGG 0.530550 7:+43351663 MsaT036746.1:CDS
AATTCGGGTCAAGCACTTGG+TGG 0.534790 7:+43352199 MsaT036746.1:CDS
AAAGGGTGTGGAAAATTCGC+GGG 0.538446 7:+43351681 MsaT036746.1:CDS
TGATGACGACGAAGCTGAAG+AGG 0.541596 7:+43351907 MsaT036746.1:CDS
AACTCGGAAAAGATCCAAAA+GGG 0.553686 7:+43351664 MsaT036746.1:CDS
AAAAGGGTGTGGAAAATTCG+CGG 0.559838 7:+43351680 MsaT036746.1:CDS
ACGGAACTTGTATCCACTTG+TGG 0.567540 7:-43351593 None:intergenic
GGAAAAGATCCAAAAGGGTG+TGG 0.567774 7:+43351669 MsaT036746.1:CDS
GAAGATTAAACCGAACATAA+CGG 0.578318 7:+43352102 MsaT036746.1:CDS
TTGAATACTCGAAAACATAC+CGG 0.586652 7:-43352178 None:intergenic
CATCTTCAATTGGAGCAGGA+AGG 0.589340 7:-43352315 None:intergenic
CATCGAAGAATAATCCAACT+CGG 0.596368 7:+43351648 MsaT036746.1:CDS
ATAATAAAGTACGTAAAGGA+AGG 0.605908 7:+43352008 MsaT036746.1:CDS
AAATCTTATCAAGCATTGGG+TGG 0.639931 7:+43352061 MsaT036746.1:CDS
AGAAGAAGAATCATCGCGAA+CGG 0.649326 7:+43351427 MsaT036746.1:CDS
TCTGATGACGATGATGAAGA+CGG 0.688378 7:-43351479 None:intergenic
GATTAAACCGAACATAACGG+TGG 0.692339 7:+43352105 MsaT036746.1:CDS
AAATGAACAATATGAAGACG+TGG 0.705726 7:+43351874 MsaT036746.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 43351389 43352399 43351389 ID=MsaG036746
Chr7 mRNA 43351389 43352399 43351389 ID=MsaT036746.1;Parent=MsaG036746
Chr7 exon 43351389 43352399 43351389 ID=MsaT036746.1.exon1;Parent=MsaT036746.1
Chr7 CDS 43351389 43352399 43351389 ID=cds.MsaT036746.1;Parent=MsaT036746.1
Gene Sequence

>MsaT036746.1

ATGAGATCTCATATGATGAACCTTCTTGTTACAAAGCAAGAAGAAGAATCATCGCGAACGGTTCAACTTAGTTTTGAAGCTGAATCAGCACCGTCTTCATCATCGTCATCAGAAGATGATGACGTTGATGAAAAGGGTCTTAATTATGGTCTTAGAGAGAATCCAAAGAGAAGCATTCGTCTCGTAGATCAAGAGTTTTCTTTTCCACAAGTGGATACAAGTTCCGTGATTCTTCAAGATATAGAAAGCGAAACTGAATCATCGAAGAATAATCCAACTCGGAAAAGATCCAAAAGGGTGTGGAAAATTCGCGGGTTTGAACAAAAGTATTACAATGAATCTGCTACGAAGAAAATGAAATTTTTTAACAAGAATGATTCTTCGGTTGTTGAACATGAACCTGTTAGTTCGGTTTCTGATGCAACAACCGAAGAAGATGTTGCATTTTGTCTAATGCAGTTGTCGAGAGACAAATGGAATAGACAAAATGAACAATATGAAGACGTGGAAGAAGAAGATGATGACGACGAAGCTGAAGAGGAAGAAGATGAAATTGAAATCGAAAGATCTTTGGAAGATAGTGATGAATCTCAAGAGCTAGTAAAAGTATGCAAGAATAATAATAAAGTACGTAAAGGAAGGTACAAATGTGAAACATGTAATAAAGTGTTCAAATCTTATCAAGCATTGGGTGGTCATAGAGCAAGTCACAAGAAGATTAAACCGAACATAACGGTGGAAGAATCGTCGCCAGAATTCGAAGTTGTTGAAAAGAAAGTTCATGAATGTCCGGTATGTTTTCGAGTATTCAATTCGGGTCAAGCACTTGGTGGACACAAAAGAACACATGTGATTCATGGATCATCATCAACAACAGTTCCAATTTTCAGCTCAAAAAGAGTTGCAAAAAGTGTTATAGATCTTAACCTTCCTGCTCCAATTGAAGATGATGAAGTTAGTCAAATTGAAAATTCTGCTGTTTCTGATGCAGAATTTGTCAAAACTCGTTAA
Protein sequence

>MsaT036746.1

MRSHMMNLLVTKQEEESSRTVQLSFEAESAPSSSSSSEDDDVDEKGLNYGLRENPKRSIRLVDQEFSFPQVDTSSVILQDIESETESSKNNPTRKRSKRVWKIRGFEQKYYNESATKKMKFFNKNDSSVVEHEPVSSVSDATTEEDVAFCLMQLSRDKWNRQNEQYEDVEEEDDDDEAEEEEDEIEIERSLEDSDESQELVKVCKNNNKVRKGRYKCETCNKVFKSYQALGGHRASHKKIKPNITVEESSPEFEVVEKKVHECPVCFRVFNSGQALGGHKRTHVIHGSSSTTVPIFSSKRVAKSVIDLNLPAPIEDDEVSQIENSAVSDAEFVKTR*