AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036766

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036766 MtrunA17_Chr7g0230771 95.251 737 19 4 1 729 4 732 0.0 1432
MsaG036766 MtrunA17_Chr7g0230731 93.351 737 30 5 1 729 4 729 0.0 1268
MsaG036766 MtrunA17_Chr4g0012071 53.545 536 191 11 209 729 60 552 4.82e-180 525
MsaG036766 MtrunA17_Chr8g0373561 35.405 370 222 11 370 729 180 542 1.33e-58 207
MsaG036766 MtrunA17_Chr2g0306641 31.316 380 227 9 369 728 122 487 4.34e-42 160
MsaG036766 MtrunA17_Chr3g0110971 27.895 380 246 7 369 728 216 587 7.23e-36 143
MsaG036766 MtrunA17_Chr8g0376381 28.877 374 235 9 369 728 166 522 9.34e-36 142
MsaG036766 MtrunA17_Chr7g0224271 27.202 386 248 9 369 729 297 674 1.31e-35 143
MsaG036766 MtrunA17_Chr4g0038761 29.539 369 232 13 375 728 87 442 8.08e-34 135
MsaG036766 MtrunA17_Chr3g0101141 26.790 377 249 9 371 728 174 542 3.33e-32 132
MsaG036766 MtrunA17_Chr4g0039661 28.606 409 266 13 335 728 158 555 2.46e-29 123
MsaG036766 MtrunA17_Chr5g0424131 29.442 394 234 16 369 729 118 500 3.99e-29 122
MsaG036766 MtrunA17_Chr5g0445331 25.201 373 260 7 373 728 162 532 1.68e-28 120
MsaG036766 MtrunA17_Chr3g0125811 26.954 371 252 8 373 728 204 570 7.45e-28 119
MsaG036766 MtrunA17_Chr3g0114841 26.933 401 237 16 369 729 117 501 9.71e-27 115
MsaG036766 MtrunA17_Chr2g0319091 25.773 388 264 9 358 727 197 578 1.81e-26 114
MsaG036766 MtrunA17_Chr7g0245601 26.776 366 242 9 375 728 441 792 4.83e-26 114
MsaG036766 MtrunA17_Chr5g0447691 22.817 355 257 7 388 728 193 544 3.35e-25 110
MsaG036766 MtrunA17_Chr1g0159461 26.400 375 249 11 373 728 226 592 3.36e-25 110
MsaG036766 MtrunA17_Chr8g0383981 26.273 373 254 9 366 727 133 495 4.38e-25 109
MsaG036766 MtrunA17_Chr2g0290241 24.804 383 252 11 371 729 56 426 5.75e-25 108
MsaG036766 MtrunA17_Chr4g0076091 24.147 381 253 9 373 729 176 544 8.83e-24 106
MsaG036766 MtrunA17_Chr4g0057291 23.607 377 266 6 369 727 289 661 1.14e-23 106
MsaG036766 MtrunA17_Chr4g0057891 25.258 388 251 12 369 729 146 521 3.12e-23 104
MsaG036766 MtrunA17_Chr5g0403741 30.303 231 148 6 506 729 51 275 6.37e-23 99.4
MsaG036766 MtrunA17_Chr1g0183641 28.000 375 240 16 369 729 120 478 9.05e-23 102
MsaG036766 MtrunA17_Chr8g0357601 22.798 386 260 10 369 728 179 552 4.59e-19 91.7
MsaG036766 MtrunA17_Chr2g0328431 23.181 371 262 9 373 727 290 653 1.24e-18 90.5
MsaG036766 MtrunA17_Chr2g0294471 23.016 378 273 9 368 728 200 576 3.15e-18 89.0
MsaG036766 MtrunA17_Chr1g0200401 26.196 397 240 15 369 727 77 458 3.80e-18 88.2
MsaG036766 MtrunA17_Chr2g0294461 23.885 381 269 9 368 728 206 585 4.62e-18 88.6
MsaG036766 MtrunA17_Chr2g0328381 24.933 373 254 11 373 727 276 640 5.69e-18 88.2
MsaG036766 MtrunA17_Chr4g0031741 23.577 369 264 7 373 727 285 649 1.92e-17 86.7
MsaG036766 MtrunA17_Chr7g0270031 24.933 373 255 11 373 728 190 554 2.56e-17 85.9
MsaG036766 MtrunA17_Chr7g0238891 22.222 378 262 6 373 728 373 740 4.55e-17 85.9
MsaG036766 MtrunA17_Chr4g0031721 22.034 413 282 10 343 727 283 683 7.53e-17 84.7
MsaG036766 MtrunA17_Chr2g0328391 22.572 381 257 9 373 727 300 668 7.55e-17 84.7
MsaG036766 MtrunA17_Chr2g0328341 23.057 386 245 10 373 727 273 637 9.45e-17 84.3
MsaG036766 MtrunA17_Chr2g0329591 23.274 391 251 10 373 728 75 451 2.38e-16 82.4
MsaG036766 MtrunA17_Chr2g0328411 22.059 408 285 10 346 727 253 653 8.96e-16 81.3
MsaG036766 MtrunA17_Chr4g0031731 22.703 370 264 9 373 723 255 621 2.26e-15 80.1
MsaG036766 MtrunA17_Chr4g0031711 21.984 373 271 7 373 727 362 732 1.34e-14 77.8
MsaG036766 MtrunA17_Chr4g0038851 33.529 170 105 2 349 510 19 188 1.88e-14 76.6
MsaG036766 MtrunA17_Chr4g0031701 21.680 369 269 7 373 723 255 621 1.99e-14 77.0
MsaG036766 MtrunA17_Chr2g0328401 21.714 350 251 7 373 706 291 633 1.29e-13 74.3
MsaG036766 MtrunA17_Chr5g0396491 27.692 195 135 2 349 541 33 223 3.14e-13 72.8
MsaG036766 MtrunA17_Chr5g0401131 21.465 396 251 11 373 728 115 490 4.56e-13 72.4
MsaG036766 MtrunA17_Chr2g0328371 24.400 250 171 6 442 678 2 246 4.37e-12 67.0
MsaG036766 MtrunA17_Chr2g0328361 24.267 375 229 14 373 727 275 614 4.71e-12 69.3
MsaG036766 MtrunA17_Chr3g0085681 31.034 145 90 2 373 511 26 166 5.87e-12 68.6
MsaG036766 MtrunA17_Chr1g0194621 23.210 405 270 16 353 728 126 518 6.78e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036766 AT4G00150.1 57.474 388 137 10 345 729 193 555 9.61e-140 422
MsaG036766 AT2G45160.1 47.974 469 192 17 292 729 193 640 2.64e-119 372
MsaG036766 AT3G60630.1 44.821 502 202 16 271 729 154 623 6.13e-114 357
MsaG036766 AT4G36710.1 32.353 374 227 12 370 728 123 485 1.86e-54 195
MsaG036766 AT2G01570.1 29.301 372 240 9 369 728 221 581 9.35e-34 137
MsaG036766 AT5G66770.1 28.499 393 258 12 351 728 200 584 3.74e-32 132
MsaG036766 AT3G03450.1 27.321 377 246 10 369 728 180 545 2.09e-31 130
MsaG036766 AT5G17490.1 28.989 376 235 13 369 728 157 516 6.47e-31 128
MsaG036766 AT2G04890.1 26.005 373 246 8 373 728 54 413 7.85e-30 123
MsaG036766 AT3G54220.1 27.778 360 247 8 375 727 296 649 2.49e-29 124
MsaG036766 AT1G14920.1 26.260 377 245 9 369 728 169 529 6.75e-29 122
MsaG036766 AT5G48150.1 24.462 372 261 8 373 728 123 490 7.50e-29 121
MsaG036766 AT5G48150.4 24.462 372 261 8 373 728 123 490 7.50e-29 121
MsaG036766 AT5G48150.2 24.462 372 261 8 373 728 123 490 7.50e-29 121
MsaG036766 AT5G48150.3 24.462 372 261 8 373 728 123 490 7.50e-29 121
MsaG036766 AT3G50650.1 29.067 375 221 12 382 728 185 542 2.30e-28 120
MsaG036766 AT5G41920.1 24.878 410 267 10 335 728 16 400 2.91e-28 118
MsaG036766 AT1G66350.1 25.606 371 251 10 369 729 152 507 1.25e-27 118
MsaG036766 AT1G50600.3 24.194 372 261 7 373 728 160 526 2.84e-27 117
MsaG036766 AT4G08250.1 29.288 379 242 14 369 727 104 476 5.88e-27 115
MsaG036766 AT1G50600.1 24.194 372 261 7 373 728 231 597 7.65e-27 116
MsaG036766 AT1G50600.2 24.194 372 261 7 373 728 235 601 7.95e-27 116
MsaG036766 AT4G17230.1 26.053 380 244 8 373 728 159 525 9.60e-27 115
MsaG036766 AT4G17230.2 26.053 380 244 8 373 728 159 525 9.60e-27 115
MsaG036766 AT1G21450.1 24.331 411 273 11 346 728 193 593 5.46e-26 114
MsaG036766 AT5G59450.1 23.095 420 282 10 339 728 190 598 4.46e-22 101
MsaG036766 AT1G55580.1 25.382 327 218 6 418 728 129 445 5.48e-20 94.0
MsaG036766 AT1G63100.1 24.495 396 246 14 369 727 249 628 1.26e-19 94.0
MsaG036766 AT1G63100.2 24.495 396 246 14 369 727 274 653 1.37e-19 94.0
MsaG036766 AT1G07520.3 21.067 375 269 8 376 727 323 693 2.82e-18 89.7
MsaG036766 AT1G07520.1 21.067 375 269 8 376 727 322 692 3.04e-18 89.7
MsaG036766 AT1G07520.2 21.067 375 269 8 376 727 158 528 3.54e-18 89.0
MsaG036766 AT1G07520.4 21.067 375 269 8 376 727 158 528 3.54e-18 89.0
MsaG036766 AT3G46600.1 21.851 389 280 7 361 728 194 579 3.72e-17 85.9
MsaG036766 AT3G46600.3 21.851 389 280 7 361 728 162 547 1.23e-16 84.3
MsaG036766 AT3G46600.2 21.892 370 272 6 373 728 5 371 2.74e-16 82.0
MsaG036766 AT1G07530.1 22.812 377 260 9 373 727 397 764 3.34e-16 83.6
MsaG036766 AT1G50420.1 29.381 194 117 5 369 551 54 238 6.08e-15 78.6
MsaG036766 AT4G37650.1 22.081 394 258 10 373 728 147 529 1.11e-14 78.2
MsaG036766 AT5G52510.1 24.204 314 192 11 450 728 338 640 3.61e-13 73.6
MsaG036766 AT2G29065.1 21.220 377 273 7 373 727 253 627 1.59e-12 71.2

Find 142 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGTGAAGAACTGAAATT+AGG 0.187840 7:+43908729 None:intergenic
GGAAAAGGTGTGTTAGATTT+TGG 0.194202 7:-43909368 MsaT036766.1:CDS
GGCAGTTGAGAAGCTTGTTT+TGG 0.246331 7:-43907455 MsaT036766.1:CDS
AAGTTACCATTTGAAGAAAT+AGG 0.249425 7:+43908858 None:intergenic
ATTTGTTCTGATTTCCCTTT+TGG 0.265105 7:-43908780 MsaT036766.1:CDS
TCTTCGCCGCCACCGCTTCC+AGG 0.267033 7:+43908351 None:intergenic
TTCCATTATCAATTTCAATA+TGG 0.276508 7:+43909007 None:intergenic
TTCCATATTGAAATTGATAA+TGG 0.285700 7:-43909009 MsaT036766.1:CDS
GCTTCCCCATAGGAGAAATT+TGG 0.291304 7:+43908214 None:intergenic
AAATGCCTCCTTAAAATAAA+AGG 0.302232 7:+43908176 None:intergenic
GTAGGAGTGGTTTGTTGATT+TGG 0.323080 7:+43909212 None:intergenic
AGATATTGAAGATGCATCTA+TGG 0.323147 7:-43908985 MsaT036766.1:CDS
GTTTATGCCTAACCCTAGTT+TGG 0.334769 7:-43908601 MsaT036766.1:CDS
GGAACACGCGCACGGGATAT+TGG 0.339508 7:-43908250 MsaT036766.1:CDS
AATGGCGCCTAAGCAGAAAA+TGG 0.344322 7:-43908379 MsaT036766.1:CDS
CAACAAGATGTTGAATTCAA+TGG 0.350188 7:-43908918 MsaT036766.1:CDS
CTTGTGAAGAACTGAAATTA+GGG 0.351845 7:+43908730 None:intergenic
AGCTTCAAGCAATGCTTGAT+TGG 0.352788 7:+43908020 None:intergenic
TGTTTGGTGCAGAGGATTCC+TGG 0.364499 7:-43907328 MsaT036766.1:CDS
TTTGCACTCCAGTCTTATTC+CGG 0.364854 7:-43907559 MsaT036766.1:CDS
AGTTACCATTTGAAGAAATA+GGG 0.368741 7:+43908859 None:intergenic
CAATCACCAAATTTCTCCTA+TGG 0.395643 7:-43908220 MsaT036766.1:CDS
TATGTATTCTTCAAAGGGTA+AGG 0.404339 7:+43909391 None:intergenic
ACTTATAGACGCCGGTAATC+CGG 0.404547 7:-43908271 MsaT036766.1:CDS
CTTGACCCTATTTCTTCAAA+TGG 0.407880 7:-43908864 MsaT036766.1:CDS
AAAGATGTGGAATGGAAGAT+TGG 0.408700 7:-43909073 MsaT036766.1:CDS
TTCTATCCTGCGAGCGCAAA+CGG 0.414907 7:+43907431 None:intergenic
AAATTTGCAAACTGCAGAAC+AGG 0.415407 7:+43908048 None:intergenic
GTGCGCGTGTTCCGGATTAC+CGG 0.417521 7:+43908260 None:intergenic
GGGAACCCTGGAAGCGGTGG+CGG 0.418143 7:-43908357 MsaT036766.1:CDS
AGTCTCTGAATTCTGTTTCG+TGG 0.422058 7:-43907762 MsaT036766.1:CDS
AATATCCCGTGCGCGTGTTC+CGG 0.422409 7:+43908252 None:intergenic
AGAGTCGCAGGCAGAGTGTT+TGG 0.429838 7:-43907344 MsaT036766.1:CDS
ATGGCGCCTAAGCAGAAAAT+GGG 0.433521 7:-43908378 MsaT036766.1:CDS
AACTTGTGGTATGTTTCTGT+TGG 0.440631 7:+43909103 None:intergenic
TCGATCATCTGAAGGACATC+CGG 0.441979 7:+43907496 None:intergenic
TAATTTGAGAAAGAGCAAAT+AGG 0.442683 7:+43907697 None:intergenic
ACTAGCCTCTTGCTCAAGAT+TGG 0.445518 7:-43908099 MsaT036766.1:CDS
ATCTCATCTCGGTTTCAACT+TGG 0.447156 7:-43907246 MsaT036766.1:CDS
TTTCCTATGTATTCTTCAAA+GGG 0.453051 7:+43909386 None:intergenic
GAGGCTGAAGCTGAATCTCT+TGG 0.460878 7:+43908544 None:intergenic
TTCATCCCTCTACTACCAAA+AGG 0.463150 7:+43908765 None:intergenic
TCACAAGTTTCACTTTCTCA+TGG 0.465348 7:-43908714 MsaT036766.1:CDS
GGTGCAGAGGATTCCTGGTA+GGG 0.470286 7:-43907323 MsaT036766.1:CDS
ATCAAGTGATTCAAGCAAAC+CGG 0.470386 7:+43907540 None:intergenic
TCGCAATCGCGTAGTGGCTG+CGG 0.474481 7:+43907739 None:intergenic
TAAAAGGCAGCCCTTTGAAA+AGG 0.479249 7:+43908192 None:intergenic
GCCTAAAATTGTCGTAACAT+TGG 0.480828 7:-43907629 MsaT036766.1:CDS
AGGGAAAATTGTTGCTATGC+GGG 0.481377 7:-43909275 MsaT036766.1:CDS
CTCGATTTCGTCGTGGTGTG+AGG 0.481816 7:+43907864 None:intergenic
CCATTGGCCTCTGCTGCAGA+TGG 0.481891 7:+43908397 None:intergenic
AAGATGTGGAATGGAAGATT+GGG 0.482044 7:-43909072 MsaT036766.1:CDS
CGCAGGATAGAACACTTCCC+TGG 0.482072 7:-43907420 MsaT036766.1:CDS
TTTGAAGAATACATAGGAAA+AGG 0.490303 7:-43909383 MsaT036766.1:CDS
TTTGGGCCGTTTGCGCTCGC+AGG 0.491743 7:-43907437 MsaT036766.1:CDS
TGGTGCAGAGGATTCCTGGT+AGG 0.496504 7:-43907324 MsaT036766.1:CDS
ACGCGATTGCGAAGCAGTTG+TGG 0.497464 7:-43907728 MsaT036766.1:CDS
AGCACTAAAGGCAGGTATGA+AGG 0.498213 7:+43907673 None:intergenic
CGAGAAATACTATATTCAAC+CGG 0.499423 7:-43907476 MsaT036766.1:CDS
ATCTTGAGCAAGAGGCTAGT+TGG 0.505941 7:+43908102 None:intergenic
AATCACCAAATTTCTCCTAT+GGG 0.509811 7:-43908219 MsaT036766.1:CDS
CGGTGGCGGCGAAGATGTAT+CGG 0.512246 7:-43908343 MsaT036766.1:CDS
GCATAAACTGAGCTTGGTGT+TGG 0.513586 7:+43908616 None:intergenic
CCATCTGCAGCAGAGGCCAA+TGG 0.514076 7:-43908397 MsaT036766.1:CDS
CGATCATCTGAAGGACATCC+GGG 0.514286 7:+43907497 None:intergenic
TCACACGGCAGCGTCGGCGG+CGG 0.515418 7:-43909167 MsaT036766.1:CDS
ACAATGGTCTTCATTTATGC+AGG 0.517675 7:-43907944 MsaT036766.1:CDS
GTGCAGAGGATTCCTGGTAG+GGG 0.518621 7:-43907322 MsaT036766.1:CDS
AGAAACATACCACAAGTTGA+AGG 0.520739 7:-43909098 MsaT036766.1:CDS
CAGCATCAACAACAATCAGT+TGG 0.520795 7:-43908678 MsaT036766.1:CDS
TTACCCTTTGAAGAATACAT+AGG 0.522370 7:-43909389 MsaT036766.1:CDS
AGCATCAACAACAATCAGTT+GGG 0.523329 7:-43908677 MsaT036766.1:CDS
AAAACAAGCTTCTCAACTGC+CGG 0.524789 7:+43907457 None:intergenic
CTTCGCCGCCACCGCTTCCA+GGG 0.526440 7:+43908352 None:intergenic
TCCTCACACGGCAGCGTCGG+CGG 0.526995 7:-43909170 MsaT036766.1:CDS
AGAACTCACTTGTCTTGTGT+TGG 0.527376 7:-43907279 MsaT036766.1:CDS
GCCGCCGACGCTGCCGTGTG+AGG 0.528406 7:+43909169 None:intergenic
CACTTGTCTTGTGTTGGCAA+AGG 0.532124 7:-43907273 MsaT036766.1:CDS
TCATCCCTCTACTACCAAAA+GGG 0.532544 7:+43908766 None:intergenic
ATGGGGAACCCTGGAAGCGG+TGG 0.534973 7:-43908360 MsaT036766.1:CDS
TGTCTTGTGTTGGCAAAGGA+AGG 0.536796 7:-43907269 MsaT036766.1:CDS
GACAGTGTTGAGGATGAAGT+AGG 0.538311 7:+43909194 None:intergenic
CAAGCAAACCGGAATAAGAC+TGG 0.539954 7:+43907551 None:intergenic
TCAAGCATTGCTTGAAGCTG+TGG 0.542939 7:-43908016 MsaT036766.1:CDS
TCCACATGGAATCCCCTACC+AGG 0.543648 7:+43907310 None:intergenic
GGTTAGGCATAAACTGAGCT+TGG 0.545303 7:+43908610 None:intergenic
GATATTGAAGATGCATCTAT+GGG 0.546979 7:-43908984 MsaT036766.1:CDS
TAGTAACTTCACAGAGTCGC+AGG 0.548006 7:-43907356 MsaT036766.1:CDS
AAGCACCAATCTTGAGCAAG+AGG 0.549041 7:+43908094 None:intergenic
CTTTGAAAAGGCTTCCCCAT+AGG 0.549307 7:+43908204 None:intergenic
CAAGTTTCACTTTCTCATGG+TGG 0.549648 7:-43908711 MsaT036766.1:CDS
TGAAGGTAAAAGATGTGGAA+TGG 0.553057 7:-43909081 MsaT036766.1:CDS
TTCCTCACCATCTGCAGCAG+AGG 0.553713 7:-43908404 MsaT036766.1:CDS
AAAATCATGTGCTGAGGAAA+CGG 0.554908 7:+43907580 None:intergenic
ATCGGCTCATCAGTTTCAGC+AGG 0.556576 7:-43908325 MsaT036766.1:CDS
TTCACGAATCGAAGCACTAA+AGG 0.557184 7:+43907661 None:intergenic
TGTTGAGGATGAAGTAGGAG+TGG 0.560505 7:+43909199 None:intergenic
TCGATTTCGTCGTGGTGTGA+GGG 0.560923 7:+43907865 None:intergenic
AGGCAGAGTGTTTGGTGCAG+AGG 0.564913 7:-43907336 MsaT036766.1:CDS
GAATGATCTTGATCTTGACT+TGG 0.565052 7:+43909029 None:intergenic
GCACTAAAGGCAGGTATGAA+GGG 0.565237 7:+43907674 None:intergenic
GGCCTCTGCTGCAGATGGTG+AGG 0.565284 7:+43908402 None:intergenic
AAGCAGAAAATGGGGAACCC+TGG 0.568423 7:-43908369 MsaT036766.1:CDS
GAGAATCTGAAGCAATATGC+TGG 0.570600 7:-43907826 MsaT036766.1:CDS
AATCTCAGCAGCAGTAGCAG+TGG 0.572943 7:+43908800 None:intergenic
GGTAATCCGGAACACGCGCA+CGG 0.575492 7:-43908258 MsaT036766.1:CDS
CAAGTTGAAGGTAAAAGATG+TGG 0.580900 7:-43909086 MsaT036766.1:CDS
AAAACCTACTCTTGTCATCA+GGG 0.582681 7:-43907396 MsaT036766.1:CDS
GCATTGCTTGAAGCTGTGGA+AGG 0.584725 7:-43908012 MsaT036766.1:CDS
CGAATCGAAGCACTAAAGGC+AGG 0.590451 7:+43907665 None:intergenic
GTAATCCGGAACACGCGCAC+GGG 0.591224 7:-43908257 MsaT036766.1:CDS
TGCAGCTGAGAATATGTGAA+AGG 0.595086 7:+43908573 None:intergenic
GCAAAGGAAGGATCTCATCT+CGG 0.596929 7:-43907257 MsaT036766.1:CDS
AGTATTTCTCGATCATCTGA+AGG 0.598913 7:+43907488 None:intergenic
GCTTCTACCGATAGCACCAC+CGG 0.599672 7:-43909143 MsaT036766.1:CDS
GGATAGGAGTTGTGATCGAA+CGG 0.600755 7:-43907608 MsaT036766.1:CDS
ATGTATTCTTCAAAGGGTAA+GGG 0.603369 7:+43909392 None:intergenic
TTATGCAGGAGTTAGCACTA+AGG 0.606201 7:-43907930 MsaT036766.1:CDS
GTCAAGAGATGTTTCTCAAG+AGG 0.606896 7:-43908458 MsaT036766.1:CDS
AGTTCTGCTTCTTCTCCACA+TGG 0.609894 7:+43907296 None:intergenic
AAAATGGGGAACCCTGGAAG+CGG 0.610138 7:-43908363 MsaT036766.1:CDS
TCTTCCTCACACGGCAGCGT+CGG 0.614368 7:-43909173 MsaT036766.1:CDS
AAATTGTCGTAACATTGGAT+AGG 0.615531 7:-43907624 MsaT036766.1:CDS
TCAACACTGTCTTCCTCACA+CGG 0.617431 7:-43909182 MsaT036766.1:CDS
ACGCGCACGGGATATTGGCG+CGG 0.617795 7:-43908245 MsaT036766.1:CDS
AGAACCCTGATGACAAGAGT+AGG 0.623547 7:+43907392 None:intergenic
CAGGAGTTAGCACTAAGGAA+TGG 0.623926 7:-43907925 MsaT036766.1:CDS
ATCACCAAATTTCTCCTATG+GGG 0.632427 7:-43908218 MsaT036766.1:CDS
TCCTGGTAGGGGATTCCATG+TGG 0.639151 7:-43907311 MsaT036766.1:CDS
ACTGCTTCGCAATCGCGTAG+TGG 0.643756 7:+43907733 None:intergenic
AATGCAGTGATCTTGAGTGC+AGG 0.644744 7:+43907895 None:intergenic
GAGGGAAAATTGTTGCTATG+CGG 0.647727 7:-43909276 MsaT036766.1:CDS
GAGTTAGCACTAAGGAATGG+TGG 0.649571 7:-43907922 MsaT036766.1:CDS
CCAGCTAAAAGACTCAATTG+TGG 0.675226 7:-43908522 MsaT036766.1:CDS
GTGAAGTTACTAAACGTCAA+CGG 0.678122 7:+43907367 None:intergenic
TGGCGCCTAAGCAGAAAATG+GGG 0.679052 7:-43908377 MsaT036766.1:CDS
TGTTAGTGTTAATGTCCACC+CGG 0.680851 7:-43907515 MsaT036766.1:CDS
TGGTTCATAACAAACTGATG+AGG 0.687444 7:+43908636 None:intergenic
GCTGCAGAACTTATAGACGC+CGG 0.689078 7:-43908279 MsaT036766.1:CDS
AACTAAGCTCGATTTCGTCG+TGG 0.700452 7:+43907857 None:intergenic
TCATCTGAAGGACATCCGGG+TGG 0.719806 7:+43907500 None:intergenic
GTGTGAGGAAGACAGTGTTG+AGG 0.771629 7:+43909184 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 43907237 43909426 43907237 ID=MsaG036766
Chr7 mRNA 43907237 43909426 43907237 ID=MsaT036766.1;Parent=MsaG036766
Chr7 exon 43907237 43909426 43907237 ID=MsaT036766.1.exon1;Parent=MsaT036766.1
Chr7 CDS 43907237 43909426 43907237 ID=cds.MsaT036766.1;Parent=MsaT036766.1
Gene Sequence

>MsaT036766.1

ATGAAGGCTATGCCCTTACCCTTTGAAGAATACATAGGAAAAGGTGTGTTAGATTTTGGTTCAAGTGTTGTTGCTTCTTCAGATTCATATTTGTTTTCACAACAGCAAGAACAGTTTTTGCAAAGGTGGAGGGAAAATTGTTGCTATGCGGGCATTGAGCCCAATTCTGTTGTTGTTTTTGACCTCAAAAAACCAAATCAACAAACCACTCCTACTTCATCCTCAACACTGTCTTCCTCACACGGCAGCGTCGGCGGCGGCGCTTCTACCGATAGCACCACCGGCGCAAAAGATAACAACACCAACAGAAACATACCACAAGTTGAAGGTAAAAGATGTGGAATGGAAGATTGGGAGAGTGTGTTATCTGAATCACCAAGTCAAGATCAAGATCATTCCATATTGAAATTGATAATGGAAGATATTGAAGATGCATCTATGGGATTAAGCAAGCTTTTACAAGTTGGAACTAGTAATTCTCATTCTCAACAAGATGTTGAATTCAATGGTTTTAGTTTTATGGATCAACAAAGTTCTGTACTTGACCCTATTTCTTCAAATGGTAACTTTGTAACGAGCATTGATTCTTCTTCTGTTGTTGCTACCACTGCTACTGCTGCTGAGATTTGTTCTGATTTCCCTTTTGGTAGTAGAGGGATGAATCAAATTCAAAACCCTAATTTCAGTTCTTCACAAGTTTCACTTTCTCATGGTGGTTTGTTTCATCAGCATCAACAACAATCAGTTGGGTCTCTTGATGAGAAGCTACCTCATCAGTTTGTTATGAACCAACACCAAGCTCAGTTTATGCCTAACCCTAGTTTGGTTTTTCCTTTCACATATTCTCAGCTGCAAGAAAACCAAGAGATTCAGCTTCAGCCTCCAGCTAAAAGACTCAATTGTGGAACGAATTATGAAATTCCAAAGATACCGTTTTTGGATTCAGGTCAAGAGATGTTTCTCAAGAGGCAACAACAACATCAGCAGCAGCTTCAACTGCTTCCTCACCATCTGCAGCAGAGGCCAATGGCGCCTAAGCAGAAAATGGGGAACCCTGGAAGCGGTGGCGGCGAAGATGTATCGGCTCATCAGTTTCAGCAGGCTATAATTGATCAGCTTTTCAAGGCTGCAGAACTTATAGACGCCGGTAATCCGGAACACGCGCACGGGATATTGGCGCGGCTCAATCACCAAATTTCTCCTATGGGGAAGCCTTTTCAAAGGGCTGCCTTTTATTTTAAGGAGGCATTTCAATTGCTTCTTCAATCAAATGTCAACAACAGCAACAACAACAGCTTTTCACCAACTAGCCTCTTGCTCAAGATTGGTGCTTATAAATCATTTTCAGAAATCTCACCTGTTCTGCAGTTTGCAAATTTCACTTCCAATCAAGCATTGCTTGAAGCTGTGGAAGGTTTCGATCGAATTCATATAATAGATTTTGATATTGGTTTTGGTGGACAATGGTCTTCATTTATGCAGGAGTTAGCACTAAGGAATGGTGGTGCACCTGCACTCAAGATCACTGCATTTGTTTCGCCCTCACACCACGACGAAATCGAGCTTAGTTTCACTAACGAGAATCTGAAGCAATATGCTGGTGAAATTAACATGTCATTTGAGCTCGAGATTCTGACACTTGAGTCTCTGAATTCTGTTTCGTGGCCGCAGCCACTACGCGATTGCGAAGCAGTTGTGGTTAACTTGCCTATTTGCTCTTTCTCAAATTACCCTTCATACCTGCCTTTAGTGCTTCGATTCGTGAAGCAGCTAATGCCTAAAATTGTCGTAACATTGGATAGGAGTTGTGATCGAACGGATGCACCGTTTCCTCAGCACATGATTTTTGCACTCCAGTCTTATTCCGGTTTGCTTGAATCACTTGATGCTGTTAGTGTTAATGTCCACCCGGATGTCCTTCAGATGATCGAGAAATACTATATTCAACCGGCAGTTGAGAAGCTTGTTTTGGGCCGTTTGCGCTCGCAGGATAGAACACTTCCCTGGAAAAACCTACTCTTGTCATCAGGGTTCTCGCCGTTGACGTTTAGTAACTTCACAGAGTCGCAGGCAGAGTGTTTGGTGCAGAGGATTCCTGGTAGGGGATTCCATGTGGAGAAGAAGCAGAACTCACTTGTCTTGTGTTGGCAAAGGAAGGATCTCATCTCGGTTTCAACTTGGAGATGCTGA
Protein sequence

>MsaT036766.1

MKAMPLPFEEYIGKGVLDFGSSVVASSDSYLFSQQQEQFLQRWRENCCYAGIEPNSVVVFDLKKPNQQTTPTSSSTLSSSHGSVGGGASTDSTTGAKDNNTNRNIPQVEGKRCGMEDWESVLSESPSQDQDHSILKLIMEDIEDASMGLSKLLQVGTSNSHSQQDVEFNGFSFMDQQSSVLDPISSNGNFVTSIDSSSVVATTATAAEICSDFPFGSRGMNQIQNPNFSSSQVSLSHGGLFHQHQQQSVGSLDEKLPHQFVMNQHQAQFMPNPSLVFPFTYSQLQENQEIQLQPPAKRLNCGTNYEIPKIPFLDSGQEMFLKRQQQHQQQLQLLPHHLQQRPMAPKQKMGNPGSGGGEDVSAHQFQQAIIDQLFKAAELIDAGNPEHAHGILARLNHQISPMGKPFQRAAFYFKEAFQLLLQSNVNNSNNNSFSPTSLLLKIGAYKSFSEISPVLQFANFTSNQALLEAVEGFDRIHIIDFDIGFGGQWSSFMQELALRNGGAPALKITAFVSPSHHDEIELSFTNENLKQYAGEINMSFELEILTLESLNSVSWPQPLRDCEAVVVNLPICSFSNYPSYLPLVLRFVKQLMPKIVVTLDRSCDRTDAPFPQHMIFALQSYSGLLESLDAVSVNVHPDVLQMIEKYYIQPAVEKLVLGRLRSQDRTLPWKNLLLSSGFSPLTFSNFTESQAECLVQRIPGRGFHVEKKQNSLVLCWQRKDLISVSTWRC*