AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036803


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036803 MtrunA17_Chr7g0230291 68.796 407 26 6 1 306 1 407 7.36e-178 504
MsaG036803 MtrunA17_Chr4g0011281 59.223 412 59 8 4 306 15 426 2.77e-155 447
MsaG036803 MtrunA17_Chr4g0073351 50.367 409 96 6 4 306 13 420 2.05e-126 373
MsaG036803 MtrunA17_Chr5g0398811 53.203 359 82 6 34 306 57 415 5.51e-113 339
MsaG036803 MtrunA17_Chr3g0121661 50.546 366 91 5 31 306 59 424 5.96e-113 339
MsaG036803 MtrunA17_Chr4g0056941 49.144 409 101 7 4 306 11 418 1.14e-112 338
MsaG036803 MtrunA17_Chr5g0396581 29.545 176 114 4 21 195 12 178 2.03e-12 68.6
MsaG036803 MtrunA17_Chr8g0386331 33.333 138 77 4 66 195 8 138 8.35e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036803 AT1G02000.1 60.291 413 55 7 2 306 6 417 1.16e-158 456
MsaG036803 AT4G00110.1 60.049 408 60 7 2 306 9 416 3.04e-157 452
MsaG036803 AT2G45310.1 56.355 417 70 7 2 306 6 422 7.04e-142 413
MsaG036803 AT3G23820.1 55.491 346 71 4 44 306 92 437 1.04e-119 357
MsaG036803 AT4G30440.1 48.522 406 106 8 3 306 9 413 1.66e-116 348
MsaG036803 AT4G12250.1 50.839 417 92 8 2 306 6 421 3.40e-112 337
MsaG036803 AT4G23920.1 35.036 137 77 3 65 195 4 134 5.88e-13 70.5
MsaG036803 AT1G12780.1 32.609 138 88 3 65 199 8 143 4.73e-12 67.4
MsaG036803 AT4G10960.1 32.847 137 80 3 65 195 5 135 1.18e-11 66.2

Find 87 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGATCTTGTTAGTATTT+TGG 0.146826 7:+44541280 MsaT036803.1:CDS
GCTTCTTCGAGTTCAGTTTA+TGG 0.231685 7:+44540853 MsaT036803.1:CDS
GCCGGTCATGGATGGGATTA+AGG 0.264379 7:+44542896 MsaT036803.1:CDS
CAATTAAGTCTAGTCCTTCT+TGG 0.272460 7:-44542827 None:intergenic
TCTCATTCATAAAGATCTAT+TGG 0.335150 7:+44542767 MsaT036803.1:intron
AACCACAACCTGCTATAGTT+TGG 0.336835 7:+44540830 MsaT036803.1:CDS
GTCCCCACCAGGTCGAAATA+TGG 0.345335 7:+44543455 MsaT036803.1:CDS
CAACGCAAATATTGATTTGA+AGG 0.345457 7:+44542100 MsaT036803.1:CDS
TTCTCCATCTTGAACTTGCC+AGG 0.365911 7:-44540256 None:intergenic
TATGTTCATAGTAACATTGC+AGG 0.375158 7:+44540772 MsaT036803.1:CDS
GTGCTTGTAACCGGCGCTGC+AGG 0.393961 7:+44540487 MsaT036803.1:CDS
GGAGAGGCTGTTGAAGGTTA+AGG 0.396204 7:+44541301 MsaT036803.1:CDS
AGGACTAGACTTAATTGATT+GGG 0.396517 7:+44542833 MsaT036803.1:CDS
ACCACAACCTGCTATAGTTT+GGG 0.398781 7:+44540831 MsaT036803.1:CDS
TCCGTAATTGTAGGTTCTTA+AGG 0.403374 7:-44540401 None:intergenic
CGTAGAGGCGACGGTGTTGT+TGG 0.407691 7:+44540544 MsaT036803.1:CDS
CACCGTCGCCTCTACGTTTG+AGG 0.417932 7:-44540537 None:intergenic
AAGGACTAGACTTAATTGAT+TGG 0.427884 7:+44542832 MsaT036803.1:CDS
TGCTGCGCCTCCGTAATTGT+AGG 0.435814 7:-44540410 None:intergenic
CCACTCTACTTCTGTCAGGC+GGG 0.447582 7:-44543345 None:intergenic
GGAGTCTCTGTGCTTGTAAC+CGG 0.459629 7:+44540478 MsaT036803.1:CDS
CGGTTCGTTCTAAAAGTGTC+TGG 0.464881 7:-44540615 None:intergenic
TCCTTAAGAACCTACAATTA+CGG 0.465555 7:+44540400 MsaT036803.1:CDS
AGGCAAATGCCGGTCATGGA+TGG 0.473023 7:+44542888 MsaT036803.1:CDS
CTTCAAACCAGTCTGTAAAT+CGG 0.484575 7:-44541423 None:intergenic
CTTCCCACTCTACTTCTGTC+AGG 0.490163 7:-44543349 None:intergenic
TTGAACTTGCCAGGTGATGA+AGG 0.495615 7:-44540247 None:intergenic
TTCCATATTTCGACCTGGTG+GGG 0.498527 7:-44543457 None:intergenic
CCTACGACCGATTTACAGAC+TGG 0.502904 7:+44541416 MsaT036803.1:CDS
ACCTTAATCCCATCCATGAC+CGG 0.504581 7:-44542897 None:intergenic
CGATAGGCAAATGCCGGTCA+TGG 0.505017 7:+44542884 MsaT036803.1:CDS
CTAGCAACAGTTCCATGGTT+CGG 0.505129 7:-44541113 None:intergenic
ACCGTCGCCTCTACGTTTGA+GGG 0.505630 7:-44540536 None:intergenic
CTGGATGATATCCGTGATGA+AGG 0.510464 7:+44543389 MsaT036803.1:CDS
TCCGACGAAACCTGCAGCGC+CGG 0.516569 7:-44540497 None:intergenic
CGAACCATGGAACTGTTGCT+AGG 0.530395 7:+44541114 MsaT036803.1:CDS
TTTCCATATTTCGACCTGGT+GGG 0.533290 7:-44543458 None:intergenic
TTACTATGAACATATGATCC+AGG 0.534976 7:-44540763 None:intergenic
GATACTGCTGAGAAGAGTAC+TGG 0.537927 7:+44541185 MsaT036803.1:CDS
CAATTACGGAGGCGCAGCAT+GGG 0.541042 7:+44540414 MsaT036803.1:CDS
AGATATGCTATGGAAAATCC+TGG 0.541942 7:+44540745 MsaT036803.1:CDS
GAAGAAGCCCAAACTATAGC+AGG 0.545446 7:-44540838 None:intergenic
TTATATTGATGATATTGTGA+GGG 0.550359 7:+44541145 MsaT036803.1:CDS
AAATGCATCACATGAGTGAA+AGG 0.550667 7:-44540703 None:intergenic
GTGATGAAGATGCCGCGGAA+CGG 0.550809 7:+44541335 MsaT036803.1:CDS
GGCAAATGCCGGTCATGGAT+GGG 0.556022 7:+44542889 MsaT036803.1:CDS
ATCACCTGGCAAGTTCAAGA+TGG 0.558738 7:+44540252 MsaT036803.1:CDS
ACAATTACGGAGGCGCAGCA+TGG 0.559108 7:+44540413 MsaT036803.1:CDS
CACTCTACTTCTGTCAGGCG+GGG 0.560608 7:-44543344 None:intergenic
GCCCAAACTATAGCAGGTTG+TGG 0.561214 7:-44540832 None:intergenic
ACCAAAATGCTAACTTTGGT+AGG 0.562854 7:-44540300 None:intergenic
AATCCCTAGCAACAGTTCCA+TGG 0.564724 7:-44541118 None:intergenic
CCCACTCTACTTCTGTCAGG+CGG 0.567730 7:-44543346 None:intergenic
ACCGGCGCTGCAGGTTTCGT+CGG 0.570051 7:+44540496 MsaT036803.1:CDS
TGGCGACCGCGTTTCAACAT+CGG 0.570679 7:-44540595 None:intergenic
CTTATATTGATGATATTGTG+AGG 0.578974 7:+44541144 MsaT036803.1:CDS
CCGCCTGACAGAAGTAGAGT+GGG 0.584369 7:+44543346 MsaT036803.1:CDS
TTGAGCTGATTCTCATCGAT+AGG 0.588143 7:+44542868 MsaT036803.1:CDS
TTGTTGAATCAGTCCCCACC+AGG 0.589026 7:+44543444 MsaT036803.1:CDS
GAACCATGGAACTGTTGCTA+GGG 0.593189 7:+44541115 MsaT036803.1:CDS
CCCGCCTGACAGAAGTAGAG+TGG 0.595822 7:+44543345 MsaT036803.1:CDS
ATGGACATTCCTTCATCACC+TGG 0.596878 7:+44540238 MsaT036803.1:CDS
CCAAAATGCTAACTTTGGTA+GGG 0.597907 7:-44540299 None:intergenic
TCTCATCGATAGGCAAATGC+CGG 0.598749 7:+44542878 MsaT036803.1:CDS
GGACTAGACTTAATTGATTG+GGG 0.603312 7:+44542834 MsaT036803.1:CDS
ATGCATTTAGCTGCACAAGC+TGG 0.612544 7:+44540718 MsaT036803.1:CDS
ATGCACAAAGAACTTTGCAA+AGG 0.613273 7:+44542179 MsaT036803.1:CDS
GTGTCTGAAGAGAATGATGA+AGG 0.614263 7:-44540367 None:intergenic
AAAGATCTATTGGAAAGACT+TGG 0.617466 7:+44542777 MsaT036803.1:intron
AGATGGAGAAAAGAACAAGA+TGG 0.619336 7:+44540269 MsaT036803.1:CDS
TTTAAGTTCATCAAGAGACA+AGG 0.625412 7:-44543417 None:intergenic
AGCTGGTGTGAGATATGCTA+TGG 0.628761 7:+44540735 MsaT036803.1:CDS
CCAGTCTGTAAATCGGTCGT+AGG 0.628896 7:-44541416 None:intergenic
GCCCTCAAACGTAGAGGCGA+CGG 0.635983 7:+44540535 MsaT036803.1:CDS
TTAAGTTCATCAAGAGACAA+GGG 0.640940 7:-44543416 None:intergenic
ACCGGCGTGTTCATTGTCGA+AGG 0.641131 7:+44540634 MsaT036803.1:CDS
AACTGAACATCTCCGTTCCG+CGG 0.643399 7:-44541347 None:intergenic
GTTTGAGGGCTATAGAGACA+TGG 0.654141 7:-44540522 None:intergenic
GCTGAGAAGAGTACTGGTAG+TGG 0.661051 7:+44541191 MsaT036803.1:CDS
TATATTGATGATATTGTGAG+GGG 0.666488 7:+44541146 MsaT036803.1:CDS
GAAATGTGATGAAGATGCCG+CGG 0.668376 7:+44541330 MsaT036803.1:CDS
GAGAAGAGTACTGGTAGTGG+TGG 0.687937 7:+44541194 MsaT036803.1:CDS
TCTATAGCCCTCAAACGTAG+AGG 0.690435 7:+44540529 MsaT036803.1:CDS
TTAAGAACCTACAATTACGG+AGG 0.706489 7:+44540403 MsaT036803.1:CDS
ATATTGATGATATTGTGAGG+GGG 0.741076 7:+44541147 MsaT036803.1:CDS
GCCTTCGACAATGAACACGC+CGG 0.748412 7:-44540635 None:intergenic
TATGATCCGATGTTGAAACG+CGG 0.786031 7:+44540589 MsaT036803.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 44540238 44543523 44540238 ID=MsaG036803
Chr7 mRNA 44540238 44543523 44540238 ID=MsaT036803.1;Parent=MsaG036803
Chr7 exon 44540238 44540308 44540238 ID=MsaT036803.1.exon1;Parent=MsaT036803.1
Chr7 CDS 44540238 44540308 44540238 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Chr7 exon 44540363 44540888 44540363 ID=MsaT036803.1.exon2;Parent=MsaT036803.1
Chr7 CDS 44540363 44540888 44540363 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Chr7 exon 44541074 44541215 44541074 ID=MsaT036803.1.exon3;Parent=MsaT036803.1
Chr7 CDS 44541074 44541215 44541074 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Chr7 exon 44541279 44541451 44541279 ID=MsaT036803.1.exon4;Parent=MsaT036803.1
Chr7 CDS 44541279 44541451 44541279 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Chr7 exon 44542083 44542200 44542083 ID=MsaT036803.1.exon5;Parent=MsaT036803.1
Chr7 CDS 44542083 44542200 44542083 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Chr7 exon 44542781 44542917 44542781 ID=MsaT036803.1.exon6;Parent=MsaT036803.1
Chr7 CDS 44542781 44542917 44542781 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Chr7 exon 44543344 44543523 44543344 ID=MsaT036803.1.exon7;Parent=MsaT036803.1
Chr7 CDS 44543344 44543523 44543344 ID=cds.MsaT036803.1;Parent=MsaT036803.1
Gene Sequence

>MsaT036803.1

ATGGACATTCCTTCATCACCTGGCAAGTTCAAGATGGAGAAAAGAACAAGATGGCATAAGTCCCTACCAAAAACACCTTCATCATTCTCTTCAGACACTTCACGTAGATCCTTAAGAACCTACAATTACGGAGGCGCAGCATGGGAAAAGAAAGTCCGTTCTTCTGCAAAAGTGACTTCCAAAAATGGAGTCTCTGTGCTTGTAACCGGCGCTGCAGGTTTCGTCGGAAGCCATGTCTCTATAGCCCTCAAACGTAGAGGCGACGGTGTTGTTGGTCTTGACAATTTCAATGACTATTATGATCCGATGTTGAAACGCGGTCGCCAGACACTTTTAGAACGAACCGGCGTGTTCATTGTCGAAGGCGATATCAATGATGCAACACTTTTGAAGAAACTTTTTGAGGTAGTTCCTTTCACTCATGTGATGCATTTAGCTGCACAAGCTGGTGTGAGATATGCTATGGAAAATCCTGGATCATATGTTCATAGTAACATTGCAGGTTTTGTTAATTTACTTGAAGTGTGTAAATCTGCTGAACCACAACCTGCTATAGTTTGGGCTTCTTCGAGTTCAGTTTATGGATTAAATTCAAAAGATATTTTGAGAGGGAAATCGATTACGATTTTTGAAGGGCCGAACCATGGAACTGTTGCTAGGGATTTTACTTATATTGATGATATTGTGAGGGGGTGTTTAGGAGCATTGGATACTGCTGAGAAGAGTACTGGTAGTGGTGTTACTGATCTTGTTAGTATTTTGGAGAGGCTGTTGAAGGTTAAGGCTAAGAGAAATGTGATGAAGATGCCGCGGAACGGAGATGTTCAGTTTACGCATGCGAATATTAGCTATGCGAAAAAAGAGTTTGGTTATAAACCTACGACCGATTTACAGACTGGTTTGAAGAAGTTTGTAAAGAATGCAGTTTACAACGCAAATATTGATTTGAAGGAAAATTTTGATCAAATCTTGGAGTACATTGAAAAGAAAAAATATGAGATCATGATCATGCACAAAGAACTTTGCAAAGATCTATTGGAAAGACTTGGTGTAGAAGCTCATACAGTGAAAAATGGCCAAGAAGGACTAGACTTAATTGATTGGGGTGCTAAACAATTTGAGCTGATTCTCATCGATAGGCAAATGCCGGTCATGGATGGGATTAAGCCCCGCCTGACAGAAGTAGAGTGGGAAGAATTTTTTAGGACGGGGCTGGATGATATCCGTGATGAAGGAAAACCCTTGTCTCTTGATGAACTTAAAGTCATTGTTGAATCAGTCCCCACCAGGTCGAAATATGGAAAAATCACATTAGGATATTATGTTATTTTTCTATATACTAAATAG

Protein sequence

>MsaT036803.1

MDIPSSPGKFKMEKRTRWHKSLPKTPSSFSSDTSRRSLRTYNYGGAAWEKKVRSSAKVTSKNGVSVLVTGAAGFVGSHVSIALKRRGDGVVGLDNFNDYYDPMLKRGRQTLLERTGVFIVEGDINDATLLKKLFEVVPFTHVMHLAAQAGVRYAMENPGSYVHSNIAGFVNLLEVCKSAEPQPAIVWASSSSVYGLNSKDILRGKSITIFEGPNHGTVARDFTYIDDIVRGCLGALDTAEKSTGSGVTDLVSILERLLKVKAKRNVMKMPRNGDVQFTHANISYAKKEFGYKPTTDLQTGLKKFVKNAVYNANIDLKENFDQILEYIEKKKYEIMIMHKELCKDLLERLGVEAHTVKNGQEGLDLIDWGAKQFELILIDRQMPVMDGIKPRLTEVEWEEFFRTGLDDIRDEGKPLSLDELKVIVESVPTRSKYGKITLGYYVIFLYTK*