AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036905

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036905 MtrunA17_Chr7g0230771 96.054 735 23 2 1 735 4 732 0.0 1449
MsaG036905 MtrunA17_Chr7g0230731 95.102 735 27 3 1 735 4 729 0.0 1431
MsaG036905 MtrunA17_Chr4g0012071 53.789 541 187 13 210 735 60 552 3.25e-179 523
MsaG036905 MtrunA17_Chr8g0373561 35.946 370 220 11 376 735 180 542 4.63e-58 206
MsaG036905 MtrunA17_Chr2g0306641 31.579 380 226 9 375 734 122 487 1.45e-41 159
MsaG036905 MtrunA17_Chr7g0224271 26.712 438 269 11 323 735 264 674 1.21e-35 144
MsaG036905 MtrunA17_Chr3g0110971 27.763 389 253 7 366 734 207 587 1.86e-35 142
MsaG036905 MtrunA17_Chr8g0376381 28.000 375 237 9 375 734 166 522 4.55e-35 140
MsaG036905 MtrunA17_Chr4g0038761 29.810 369 231 13 381 734 87 442 1.06e-33 135
MsaG036905 MtrunA17_Chr3g0101141 26.525 377 250 9 377 734 174 542 2.13e-31 129
MsaG036905 MtrunA17_Chr4g0039661 28.642 405 271 12 341 734 158 555 8.36e-29 121
MsaG036905 MtrunA17_Chr5g0424131 29.188 394 235 15 375 735 118 500 1.19e-28 120
MsaG036905 MtrunA17_Chr5g0445331 24.933 373 261 7 379 734 162 532 8.76e-28 118
MsaG036905 MtrunA17_Chr3g0125811 27.419 372 249 9 379 734 204 570 5.14e-27 116
MsaG036905 MtrunA17_Chr3g0114841 26.933 401 237 16 375 735 117 501 4.78e-26 112
MsaG036905 MtrunA17_Chr7g0245601 27.049 366 241 9 381 734 441 792 5.99e-26 114
MsaG036905 MtrunA17_Chr2g0319091 25.538 372 254 8 379 733 213 578 3.73e-25 110
MsaG036905 MtrunA17_Chr5g0447691 22.817 355 257 7 394 734 193 544 7.98e-25 109
MsaG036905 MtrunA17_Chr1g0159461 26.596 376 247 12 379 734 226 592 9.41e-25 109
MsaG036905 MtrunA17_Chr8g0383981 26.542 373 253 9 372 733 133 495 1.09e-24 108
MsaG036905 MtrunA17_Chr2g0290241 24.804 383 252 11 377 735 56 426 4.47e-24 105
MsaG036905 MtrunA17_Chr4g0057291 23.810 378 264 7 375 733 289 661 7.25e-24 107
MsaG036905 MtrunA17_Chr5g0403741 31.897 232 143 7 512 735 51 275 1.95e-23 100
MsaG036905 MtrunA17_Chr1g0183641 28.267 375 239 16 375 735 120 478 1.71e-22 101
MsaG036905 MtrunA17_Chr4g0057891 25.258 388 251 12 375 735 146 521 1.72e-22 102
MsaG036905 MtrunA17_Chr4g0076091 23.622 381 255 9 379 735 176 544 2.14e-22 102
MsaG036905 MtrunA17_Chr8g0357601 22.798 386 260 10 375 734 179 552 2.43e-18 89.4
MsaG036905 MtrunA17_Chr2g0328431 22.911 371 263 9 379 733 290 653 1.60e-17 87.0
MsaG036905 MtrunA17_Chr2g0328381 24.933 373 254 11 379 733 276 640 1.63e-17 87.0
MsaG036905 MtrunA17_Chr1g0200401 25.935 401 236 16 375 733 77 458 2.87e-17 85.5
MsaG036905 MtrunA17_Chr2g0294471 23.454 388 272 10 364 734 197 576 2.92e-17 85.9
MsaG036905 MtrunA17_Chr2g0294461 23.885 381 269 9 374 734 206 585 3.23e-17 85.9
MsaG036905 MtrunA17_Chr7g0270031 25.067 371 257 11 379 734 190 554 1.30e-16 84.0
MsaG036905 MtrunA17_Chr2g0328391 22.835 381 256 9 379 733 300 668 2.28e-16 83.2
MsaG036905 MtrunA17_Chr2g0328341 23.057 386 245 10 379 733 273 637 2.74e-16 83.2
MsaG036905 MtrunA17_Chr7g0238891 22.938 388 247 8 379 734 373 740 3.70e-16 82.8
MsaG036905 MtrunA17_Chr4g0031741 22.764 369 267 6 379 733 285 649 5.18e-16 82.0
MsaG036905 MtrunA17_Chr4g0031721 21.550 413 284 10 349 733 283 683 6.26e-16 82.0
MsaG036905 MtrunA17_Chr2g0329591 22.762 391 253 10 379 734 75 451 2.07e-15 79.3
MsaG036905 MtrunA17_Chr2g0328411 24.815 270 179 6 352 601 253 518 7.69e-15 78.6
MsaG036905 MtrunA17_Chr4g0038851 33.529 170 105 2 355 516 19 188 1.47e-14 77.0
MsaG036905 MtrunA17_Chr4g0031731 22.162 370 266 9 379 729 255 621 2.05e-14 77.0
MsaG036905 MtrunA17_Chr4g0031701 21.138 369 271 7 379 729 255 621 1.20e-13 74.7
MsaG036905 MtrunA17_Chr2g0328401 25.000 236 157 6 379 601 291 519 2.01e-13 73.9
MsaG036905 MtrunA17_Chr4g0031711 21.448 373 273 7 379 733 362 732 2.73e-13 73.6
MsaG036905 MtrunA17_Chr5g0396491 27.692 195 135 2 355 547 33 223 3.02e-13 72.8
MsaG036905 MtrunA17_Chr5g0401131 21.465 396 251 11 379 734 115 490 6.97e-13 71.6
MsaG036905 MtrunA17_Chr7g0236851 26.010 396 218 17 379 733 295 656 7.07e-13 72.0
MsaG036905 MtrunA17_Chr3g0085681 31.034 145 90 2 379 517 26 166 6.04e-12 68.6
MsaG036905 MtrunA17_Chr2g0328371 24.400 250 171 6 448 684 2 246 1.30e-11 65.5
MsaG036905 MtrunA17_Chr2g0328361 24.000 375 230 14 379 733 275 614 3.13e-11 66.6
MsaG036905 MtrunA17_Chr1g0194621 23.457 405 269 16 359 734 126 518 3.46e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036905 AT4G00150.1 56.818 396 141 11 343 735 187 555 5.11e-139 421
MsaG036905 AT2G45160.1 47.854 466 202 16 293 735 193 640 6.44e-120 374
MsaG036905 AT3G60630.1 44.970 507 199 17 272 735 154 623 2.20e-113 356
MsaG036905 AT4G36710.1 32.620 374 226 12 376 734 123 485 4.75e-54 194
MsaG036905 AT2G01570.1 29.570 372 239 9 375 734 221 581 2.83e-33 136
MsaG036905 AT3G03450.1 27.202 386 253 10 366 734 171 545 5.23e-31 129
MsaG036905 AT5G66770.1 27.990 393 260 12 357 734 200 584 5.74e-31 129
MsaG036905 AT5G17490.1 29.067 375 236 12 375 734 157 516 2.13e-30 126
MsaG036905 AT3G54220.1 28.056 360 246 8 381 733 296 649 4.80e-29 123
MsaG036905 AT2G04890.1 25.469 373 248 8 379 734 54 413 6.70e-29 120
MsaG036905 AT1G14920.1 26.525 377 244 9 375 734 169 529 1.56e-28 121
MsaG036905 AT5G48150.1 24.194 372 262 8 379 734 123 490 4.89e-28 119
MsaG036905 AT5G48150.4 24.194 372 262 8 379 734 123 490 4.89e-28 119
MsaG036905 AT5G48150.2 24.194 372 262 8 379 734 123 490 4.89e-28 119
MsaG036905 AT5G48150.3 24.194 372 262 8 379 734 123 490 4.89e-28 119
MsaG036905 AT5G41920.1 24.634 410 268 10 341 734 16 400 1.85e-27 115
MsaG036905 AT3G50650.1 28.533 375 223 12 388 734 185 542 3.00e-27 117
MsaG036905 AT1G66350.1 25.876 371 250 10 375 735 152 507 3.83e-27 116
MsaG036905 AT1G50600.3 23.656 372 263 7 379 734 160 526 3.23e-26 114
MsaG036905 AT4G17230.1 25.789 380 245 8 379 734 159 525 8.21e-26 112
MsaG036905 AT4G17230.2 25.789 380 245 8 379 734 159 525 8.21e-26 112
MsaG036905 AT1G50600.1 23.656 372 263 7 379 734 231 597 9.78e-26 113
MsaG036905 AT1G50600.2 23.656 372 263 7 379 734 235 601 1.01e-25 113
MsaG036905 AT1G21450.1 24.574 411 272 11 352 734 193 593 1.52e-25 112
MsaG036905 AT4G08250.1 28.496 379 245 14 375 733 104 476 2.01e-25 110
MsaG036905 AT5G59450.1 22.619 420 284 10 345 734 190 598 3.88e-21 98.6
MsaG036905 AT1G63100.1 24.242 396 247 14 375 733 249 628 1.67e-19 93.6
MsaG036905 AT1G63100.2 23.797 395 250 12 375 733 274 653 1.71e-19 93.6
MsaG036905 AT1G55580.1 24.465 327 221 6 424 734 129 445 5.72e-19 90.9
MsaG036905 AT1G07520.3 21.011 376 268 9 382 733 323 693 2.00e-17 87.0
MsaG036905 AT1G07520.1 21.011 376 268 9 382 733 322 692 2.17e-17 87.0
MsaG036905 AT1G07520.2 20.800 375 270 8 382 733 158 528 2.43e-17 86.3
MsaG036905 AT1G07520.4 20.800 375 270 8 382 733 158 528 2.43e-17 86.3
MsaG036905 AT1G07530.1 22.812 377 260 9 379 733 397 764 1.79e-15 81.3
MsaG036905 AT3G46600.1 21.351 370 274 6 379 734 213 579 1.83e-15 80.5
MsaG036905 AT3G46600.3 21.351 370 274 6 379 734 181 547 4.99e-15 79.3
MsaG036905 AT3G46600.2 26.857 175 126 1 379 551 5 179 5.33e-15 77.8
MsaG036905 AT1G50420.1 29.381 194 117 5 375 557 54 238 8.54e-15 78.2
MsaG036905 AT4G37650.1 21.446 401 252 10 379 734 147 529 6.00e-14 75.9
MsaG036905 AT5G52510.1 23.567 314 194 11 456 734 338 640 7.85e-12 69.3
MsaG036905 AT2G29065.1 20.690 377 275 7 379 733 253 627 3.30e-11 67.0

Find 155 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGTGAAGAACTGAAATT+AGG 0.187840 7:-47052047 None:intergenic
GGAAAAGGTGTGTTAGATTT+TGG 0.194202 7:+47051405 MsaT036905.1:CDS
TTGTTACCATTTGAAGAAAT+AGG 0.224501 7:-47051915 None:intergenic
GACAATTGAGAAGCTTGTTT+TGG 0.255336 7:+47053336 MsaT036905.1:CDS
AGTCTCTGAATTCTGTTTCA+TGG 0.261651 7:+47053029 MsaT036905.1:CDS
TCTTCGCCGCCACCGCTTCC+AGG 0.267033 7:-47052440 None:intergenic
TTCCATTATCAATTTCAATA+TGG 0.276508 7:-47051766 None:intergenic
AATTCCGAAGATACCCTTCT+TGG 0.278051 7:+47052289 MsaT036905.1:CDS
TTCCATATTGAAATTGATAA+TGG 0.285700 7:+47051764 MsaT036905.1:CDS
GCTTCCCCATAGGAGAAATT+TGG 0.291304 7:-47052577 None:intergenic
AAATGCCTCCTTAAAATAAA+AGG 0.302232 7:-47052615 None:intergenic
GCATAAACTGAGCTTGGTTT+TGG 0.318676 7:-47052160 None:intergenic
GTAGGAGTGGTTTGTTGATT+TGG 0.323080 7:-47051561 None:intergenic
AGATATTGAAGATGCATCTA+TGG 0.323147 7:+47051788 MsaT036905.1:CDS
GTTTATGCCTAACCCTAGTT+TGG 0.334769 7:+47052175 MsaT036905.1:CDS
GGAACACGCGCACGGGATAT+TGG 0.339508 7:+47052541 MsaT036905.1:CDS
GAAGATACCCTTCTTGGATT+CGG 0.340738 7:+47052295 MsaT036905.1:CDS
AATGGCGCCTAAGCAGAAAA+TGG 0.344322 7:+47052412 MsaT036905.1:CDS
CAACAAGATGTTGAATTCAA+TGG 0.350188 7:+47051855 MsaT036905.1:CDS
CTTGTGAAGAACTGAAATTA+GGG 0.351845 7:-47052046 None:intergenic
CAGCATCAACAACAATCAAT+TGG 0.353717 7:+47052098 MsaT036905.1:CDS
AAGATACCCTTCTTGGATTC+GGG 0.361865 7:+47052296 MsaT036905.1:CDS
TGTTACCATTTGAAGAAATA+GGG 0.362144 7:-47051914 None:intergenic
TGTTTGGTGCAGAGGATTCC+TGG 0.364499 7:+47053463 MsaT036905.1:CDS
TTTGCACTCCAGTCTTATTC+CGG 0.364854 7:+47053232 MsaT036905.1:CDS
CGGGTCAAGAGATGTTTCTT+AGG 0.388186 7:+47052315 MsaT036905.1:CDS
ATCGGTAGAAGCGCCGCCTC+CGG 0.394887 7:-47051619 None:intergenic
CAATCACCAAATTTCTCCTA+TGG 0.395643 7:+47052571 MsaT036905.1:CDS
AAGATGTGGAGTGGAGGATT+GGG 0.397037 7:+47051701 MsaT036905.1:CDS
CGAAGATGTATCGGCTCATC+AGG 0.401016 7:+47052457 MsaT036905.1:CDS
ACTTATAGACGCCGGTAATC+CGG 0.404547 7:+47052520 MsaT036905.1:CDS
CTTGACCCTATTTCTTCAAA+TGG 0.407880 7:+47051909 MsaT036905.1:CDS
TTTCCTATGTATTCTTCAAA+AGG 0.413997 7:-47051387 None:intergenic
AAAGATGTGGAGTGGAGGAT+TGG 0.414318 7:+47051700 MsaT036905.1:CDS
TTCTATCCTGCGAGCGCAAA+CGG 0.414907 7:-47053360 None:intergenic
AAATTTGCAAACTGCAGAAC+AGG 0.415407 7:-47052743 None:intergenic
TATGTATTCTTCAAAAGGTA+AGG 0.415901 7:-47051382 None:intergenic
GTGCGCGTGTTCCGGATTAC+CGG 0.417521 7:-47052531 None:intergenic
GGGAACCCTGGAAGCGGTGG+CGG 0.418143 7:+47052434 MsaT036905.1:CDS
AATATCCCGTGCGCGTGTTC+CGG 0.422409 7:-47052539 None:intergenic
AAAACAAGCTTCTCAATTGT+CGG 0.427465 7:-47053334 None:intergenic
AGAGTCGCAGGCAGAGTGTT+TGG 0.429838 7:+47053447 MsaT036905.1:CDS
ATGGCGCCTAAGCAGAAAAT+GGG 0.433521 7:+47052413 MsaT036905.1:CDS
CCACAGCTTCAAGCAATGCT+TGG 0.439953 7:-47052775 None:intergenic
CTCAATTTCGTCGTGGTGTG+AGG 0.440067 7:-47052927 None:intergenic
TCGATCATCTGAAGGACATC+CGG 0.441979 7:-47053295 None:intergenic
TTCATCCCTCTACTAGCAAA+AGG 0.442041 7:-47052011 None:intergenic
TAATTTGAGAAAGAGCAAAT+AGG 0.442683 7:-47053094 None:intergenic
AGCTTGTGGTATGTTTCTGT+TGG 0.443296 7:-47051670 None:intergenic
ACTAGCCTCTTGCTCAAGAT+TGG 0.445518 7:+47052692 MsaT036905.1:CDS
ATCTCATCTCGGTTTCAACT+TGG 0.447156 7:+47053545 MsaT036905.1:CDS
TTTGAAGAATACATAGGAAA+AGG 0.459740 7:+47051390 MsaT036905.1:CDS
GAGGCTGAAGCTGAATCTCT+TGG 0.460878 7:-47052232 None:intergenic
TCACAAGTTTCACTTTCTCA+TGG 0.465348 7:+47052062 MsaT036905.1:CDS
GGTGCAGAGGATTCCTGGTA+GGG 0.470286 7:+47053468 MsaT036905.1:CDS
TCGCAATCGCGTAGTGGCTG+CGG 0.474481 7:-47053052 None:intergenic
AAAATCATATGCTGAGGAAC+CGG 0.475265 7:-47053211 None:intergenic
GGTTAGGCATAAACTGAGCT+TGG 0.478491 7:-47052166 None:intergenic
TAAAAGGCAGCCCTTTGAAA+AGG 0.479249 7:-47052599 None:intergenic
AGGGAAAATTGTTGCTATGC+GGG 0.481377 7:+47051498 MsaT036905.1:CDS
CGCAGGATAGAACACTTCCC+TGG 0.482072 7:+47053371 MsaT036905.1:CDS
AGCACTAAAGGTAGGTATGA+AGG 0.484386 7:-47053118 None:intergenic
AGCTTCAAGCAATGCTTGGT+TGG 0.488113 7:-47052771 None:intergenic
AGCATCAACAACAATCAATT+GGG 0.491428 7:+47052099 MsaT036905.1:CDS
TTTGGGCCGTTTGCGCTCGC+AGG 0.491743 7:+47053354 MsaT036905.1:CDS
GCCTAAAATCGTCGTAACAT+TGG 0.494379 7:+47053162 MsaT036905.1:CDS
ATCAAGTGATTCGAGCAAAC+CGG 0.496065 7:-47053251 None:intergenic
TGGTGCAGAGGATTCCTGGT+AGG 0.496504 7:+47053467 MsaT036905.1:CDS
ACGCGATTGCGAAGCAGTTG+TGG 0.497464 7:+47053063 MsaT036905.1:CDS
TCACACGGTAGCGCCGGAGG+CGG 0.497760 7:+47051606 MsaT036905.1:CDS
CTTTGCGCCGGTGGTGCTAT+CGG 0.499180 7:-47051637 None:intergenic
ACAATGGTCTTCATTTATGC+AGG 0.504102 7:+47052847 MsaT036905.1:CDS
ATCTTGAGCAAGAGGCTAGT+TGG 0.505941 7:-47052689 None:intergenic
AATCACCAAATTTCTCCTAT+GGG 0.509811 7:+47052572 MsaT036905.1:CDS
GAATCCAAGAAGGGTATCTT+CGG 0.510787 7:-47052293 None:intergenic
CGGTGGCGGCGAAGATGTAT+CGG 0.512246 7:+47052448 MsaT036905.1:CDS
CGATCATCTGAAGGACATCC+GGG 0.514286 7:-47053294 None:intergenic
GTGCAGAGGATTCCTGGTAG+GGG 0.518621 7:+47053469 MsaT036905.1:CDS
CCAAGCATTGCTTGAAGCTG+TGG 0.524919 7:+47052775 MsaT036905.1:CDS
CTTCGCCGCCACCGCTTCCA+GGG 0.526440 7:-47052439 None:intergenic
ATCGGCTCATCAGGTTCAGC+AGG 0.527235 7:+47052466 MsaT036905.1:CDS
AGAACTCACTTGTCTTGTGT+TGG 0.527376 7:+47053512 MsaT036905.1:CDS
CACTTGTCTTGTGTTGGCAA+AGG 0.532124 7:+47053518 MsaT036905.1:CDS
ATGGGGAACCCTGGAAGCGG+TGG 0.534973 7:+47052431 MsaT036905.1:CDS
TCATCCCTCTACTAGCAAAA+GGG 0.536599 7:-47052010 None:intergenic
TGTCTTGTGTTGGCAAAGGA+AGG 0.536796 7:+47053522 MsaT036905.1:CDS
GACAGTGTTGAGGATGAAGT+AGG 0.538311 7:-47051579 None:intergenic
CTCTTGACCCGAATCCAAGA+AGG 0.539089 7:-47052303 None:intergenic
CGAGCAAACCGGAATAAGAC+TGG 0.539561 7:-47053240 None:intergenic
GCACTAAAGGTAGGTATGAA+GGG 0.542279 7:-47053117 None:intergenic
TCCACATGGAATCCCCTACC+AGG 0.543648 7:-47053481 None:intergenic
GATATTGAAGATGCATCTAT+GGG 0.546979 7:+47051789 MsaT036905.1:CDS
TAGTAACTTCACAGAGTCGC+AGG 0.548006 7:+47053435 MsaT036905.1:CDS
ATTGTTATCCTTTGCGCCGG+TGG 0.548468 7:-47051646 None:intergenic
AAGCACCAATCTTGAGCAAG+AGG 0.549041 7:-47052697 None:intergenic
CTTTGAAAAGGCTTCCCCAT+AGG 0.549307 7:-47052587 None:intergenic
CAAGTTTCACTTTCTCATGG+TGG 0.549648 7:+47052065 MsaT036905.1:CDS
GCCTCCGGCGCTACCGTGTG+AGG 0.550488 7:-47051604 None:intergenic
TTGTGATCGAACGGATGCAC+CGG 0.554827 7:+47053192 MsaT036905.1:CDS
CCATTGGCCTATGCTGCAGA+TGG 0.558131 7:-47052394 None:intergenic
TGTTGAGGATGAAGTAGGAG+TGG 0.560505 7:-47051574 None:intergenic
TCAATTTCGTCGTGGTGTGA+GGG 0.560921 7:-47052926 None:intergenic
TTCACGAATCGAAGCACTAA+AGG 0.562327 7:-47053130 None:intergenic
AACAGAAGAAGAATCAATGC+TGG 0.562661 7:-47051952 None:intergenic
GTCAAGAGATGTTTCTTAGG+AGG 0.562943 7:+47052318 MsaT036905.1:CDS
AGGCAGAGTGTTTGGTGCAG+AGG 0.564913 7:+47053455 MsaT036905.1:CDS
GAATGATCTTGATCTTGACT+TGG 0.565052 7:-47051744 None:intergenic
AAGCAGAAAATGGGGAACCC+TGG 0.568423 7:+47052422 MsaT036905.1:CDS
TTCCTCACCATCTGCAGCAT+AGG 0.569164 7:+47052387 MsaT036905.1:CDS
TCCTCACACGGTAGCGCCGG+AGG 0.569283 7:+47051603 MsaT036905.1:CDS
AAATCGTCGTAACATTGGAT+AGG 0.569644 7:+47053167 MsaT036905.1:CDS
TCTTCCTCACACGGTAGCGC+CGG 0.570460 7:+47051600 MsaT036905.1:CDS
GAGAATCTGAAGCAATATGC+TGG 0.570600 7:+47052965 MsaT036905.1:CDS
TTATGCAGGAGTTAGCACTT+AGG 0.570667 7:+47052861 MsaT036905.1:CDS
GGTAATCCGGAACACGCGCA+CGG 0.575492 7:+47052533 MsaT036905.1:CDS
AAAACCTACTCTTGTCATCA+GGG 0.582681 7:+47053395 MsaT036905.1:CDS
GGTATTGTTATCCTTTGCGC+CGG 0.583054 7:-47051649 None:intergenic
GCATTGCTTGAAGCTGTGGA+AGG 0.584725 7:+47052779 MsaT036905.1:CDS
CGATAGCACCACCGGCGCAA+AGG 0.585841 7:+47051638 MsaT036905.1:CDS
CCATCTGCAGCATAGGCCAA+TGG 0.588621 7:+47052394 MsaT036905.1:CDS
GTAATCCGGAACACGCGCAC+GGG 0.591224 7:+47052534 MsaT036905.1:CDS
GCAAAGGAAGGATCTCATCT+CGG 0.596929 7:+47053534 MsaT036905.1:CDS
AGTATTTCTCGATCATCTGA+AGG 0.598913 7:-47053303 None:intergenic
GCTTCTACCGATAGCACCAC+CGG 0.599672 7:+47051630 MsaT036905.1:CDS
GGATAGGAGTTGTGATCGAA+CGG 0.600755 7:+47053183 MsaT036905.1:CDS
ATGTATTCTTCAAAAGGTAA+GGG 0.601264 7:-47051381 None:intergenic
CGAATCGAAGCACTAAAGGT+AGG 0.602747 7:-47053126 None:intergenic
AGGTAAAAGATGTGGAGTGG+AGG 0.606059 7:+47051695 MsaT036905.1:CDS
AGTTCTGCTTCTTCTCCACA+TGG 0.609894 7:-47053495 None:intergenic
AAAATGGGGAACCCTGGAAG+CGG 0.610138 7:+47052428 MsaT036905.1:CDS
TGCAGCTGAGAGTGTGTGAA+AGG 0.610994 7:-47052203 None:intergenic
CAAGCTGAAGGTAAAAGATG+TGG 0.613564 7:+47051687 MsaT036905.1:CDS
ACGCGCACGGGATATTGGCG+CGG 0.617795 7:+47052546 MsaT036905.1:CDS
AGAACCCTGATGACAAGAGT+AGG 0.623547 7:-47053399 None:intergenic
CAGGAGTTAGCACTTAGGAA+TGG 0.623926 7:+47052866 MsaT036905.1:CDS
GGCCTATGCTGCAGATGGTG+AGG 0.630076 7:-47052389 None:intergenic
TCTTGACCCGAATCCAAGAA+GGG 0.632079 7:-47052302 None:intergenic
ATCACCAAATTTCTCCTATG+GGG 0.632427 7:+47052573 MsaT036905.1:CDS
TCAACACTGTCTTCCTCACA+CGG 0.633334 7:+47051591 MsaT036905.1:CDS
TGAAGGTAAAAGATGTGGAG+TGG 0.636742 7:+47051692 MsaT036905.1:CDS
TGGTTCATAACAAACTGATG+AGG 0.638887 7:-47052140 None:intergenic
TCCTGGTAGGGGATTCCATG+TGG 0.639151 7:+47053480 MsaT036905.1:CDS
AGAAACATACCACAAGCTGA+AGG 0.641675 7:+47051675 MsaT036905.1:CDS
ACTGCTTCGCAATCGCGTAG+TGG 0.643756 7:-47053058 None:intergenic
AATGCAGTGATCTTGAGTGC+AGG 0.644744 7:-47052896 None:intergenic
GAGTTAGCACTTAGGAATGG+TGG 0.645397 7:+47052869 MsaT036905.1:CDS
GAGGGAAAATTGTTGCTATG+CGG 0.647727 7:+47051497 MsaT036905.1:CDS
CCAGCTAAAAGACTCAATTG+TGG 0.675226 7:+47052254 MsaT036905.1:CDS
GTGAAGTTACTAAACGTCAA+CGG 0.678122 7:-47053424 None:intergenic
TGGCGCCTAAGCAGAAAATG+GGG 0.679052 7:+47052414 MsaT036905.1:CDS
TGTTAGTGTCAATGTCCACC+CGG 0.680851 7:+47053276 MsaT036905.1:CDS
GCTGCAGAACTTATAGACGC+CGG 0.689078 7:+47052512 MsaT036905.1:CDS
AACTGAGCTCAATTTCGTCG+TGG 0.691423 7:-47052934 None:intergenic
TCATCTGAAGGACATCCGGG+TGG 0.719806 7:-47053291 None:intergenic
GTGTGAGGAAGACAGTGTTG+AGG 0.783398 7:-47051589 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 47051369 47053576 47051369 ID=MsaG036905
Chr7 mRNA 47051369 47053576 47051369 ID=MsaT036905.1;Parent=MsaG036905
Chr7 exon 47051369 47053576 47051369 ID=MsaT036905.1.exon1;Parent=MsaT036905.1
Chr7 CDS 47051369 47053576 47051369 ID=cds.MsaT036905.1;Parent=MsaT036905.1
Gene Sequence

>MsaT036905.1

ATGAAGGCTATGCCCTTACCTTTTGAAGAATACATAGGAAAAGGTGTGTTAGATTTTGGTTCAAGTGTTGTTGCTTCTTCAGATTCATATTTGTTTTCACAACAGCAAGAACAGTTTTTGCAAAGGTGGAGGGAAAATTGTTGCTATGCGGGCATTGAGCCCAATTCTGTTGTTGTTTTTGACCTCAAAAAACCAAATCAACAAACCACTCCTACTTCATCCTCAACACTGTCTTCCTCACACGGTAGCGCCGGAGGCGGCGCTTCTACCGATAGCACCACCGGCGCAAAGGATAACAATACCAACAGAAACATACCACAAGCTGAAGGTAAAAGATGTGGAGTGGAGGATTGGGAGAGTGTGTTATCTGAATCACCAAGTCAAGATCAAGATCATTCCATATTGAAATTGATAATGGAAGATATTGAAGATGCATCTATGGGATTAAGCAAGCTTTTACAAGTTGGAACTAGTAATTCTCATTCTCAACAAGATGTTGAATTCAATGGTTTTAGTTTTATGGATCAACAAAGTTCTGTACTTGACCCTATTTCTTCAAATGGTAACAACAATAACTTTGTAACCAGCATTGATTCTTCTTCTGTTATTGCTACTACTGCTGCTGAGATTTGTTCTGATTTCCCTTTTGCTAGTAGAGGGATGAATCAAATTCAAAACCCTAATTTCAGTTCTTCACAAGTTTCACTTTCTCATGGTGGTTTGTTTCATCAGCATCAACAACAATCAATTGGGTCTCTTGATGAGAAACTACCTCATCAGTTTGTTATGAACCAAAACCAAGCTCAGTTTATGCCTAACCCTAGTTTGGTTTTTCCTTTCACACACTCTCAGCTGCAAGAAAACCAAGAGATTCAGCTTCAGCCTCCAGCTAAAAGACTCAATTGTGGAACGAATTATGAAATTCCGAAGATACCCTTCTTGGATTCGGGTCAAGAGATGTTTCTTAGGAGGCAACAACAGCATCAGCAACAGCAACATCAGCAGCAGCTTCAACTGCTTCCTCACCATCTGCAGCATAGGCCAATGGCGCCTAAGCAGAAAATGGGGAACCCTGGAAGCGGTGGCGGCGAAGATGTATCGGCTCATCAGGTTCAGCAGGCTATAATTGATCAGCTTTTCAAGGCTGCAGAACTTATAGACGCCGGTAATCCGGAACACGCGCACGGGATATTGGCGCGGCTCAATCACCAAATTTCTCCTATGGGGAAGCCTTTTCAAAGGGCTGCCTTTTATTTTAAGGAGGCATTTCAATTGCTTCTTCAATCAAATGTCAACAACAGCAACAACAACAGCTTTTCACCAACTAGCCTCTTGCTCAAGATTGGTGCTTATAAATCATTCTCAGAAATCTCACCTGTTCTGCAGTTTGCAAATTTCACTTCCAACCAAGCATTGCTTGAAGCTGTGGAAGGTTTTGATCGAATTCATATAATAGATTTTGATATTGGTTTTGGCGGACAATGGTCTTCATTTATGCAGGAGTTAGCACTTAGGAATGGTGGTGCACCTGCACTCAAGATCACTGCATTTGTTTCGCCCTCACACCACGACGAAATTGAGCTCAGTTTCACTAACGAGAATCTGAAGCAATATGCTGGTGAAATTAACATGTCATTTGAGCTCGAGATTCTGACACTTGAGTCTCTGAATTCTGTTTCATGGCCGCAGCCACTACGCGATTGCGAAGCAGTTGTGGTTAACTTGCCTATTTGCTCTTTCTCAAATTACCCTTCATACCTACCTTTAGTGCTTCGATTCGTGAAGCAGCTCATGCCTAAAATCGTCGTAACATTGGATAGGAGTTGTGATCGAACGGATGCACCGGTTCCTCAGCATATGATTTTTGCACTCCAGTCTTATTCCGGTTTGCTCGAATCACTTGATGCTGTTAGTGTCAATGTCCACCCGGATGTCCTTCAGATGATCGAGAAATACTATCTTCAACCGACAATTGAGAAGCTTGTTTTGGGCCGTTTGCGCTCGCAGGATAGAACACTTCCCTGGAAAAACCTACTCTTGTCATCAGGGTTCTCGCCGTTGACGTTTAGTAACTTCACAGAGTCGCAGGCAGAGTGTTTGGTGCAGAGGATTCCTGGTAGGGGATTCCATGTGGAGAAGAAGCAGAACTCACTTGTCTTGTGTTGGCAAAGGAAGGATCTCATCTCGGTTTCAACTTGGAGATGCTGA
Protein sequence

>MsaT036905.1

MKAMPLPFEEYIGKGVLDFGSSVVASSDSYLFSQQQEQFLQRWRENCCYAGIEPNSVVVFDLKKPNQQTTPTSSSTLSSSHGSAGGGASTDSTTGAKDNNTNRNIPQAEGKRCGVEDWESVLSESPSQDQDHSILKLIMEDIEDASMGLSKLLQVGTSNSHSQQDVEFNGFSFMDQQSSVLDPISSNGNNNNFVTSIDSSSVIATTAAEICSDFPFASRGMNQIQNPNFSSSQVSLSHGGLFHQHQQQSIGSLDEKLPHQFVMNQNQAQFMPNPSLVFPFTHSQLQENQEIQLQPPAKRLNCGTNYEIPKIPFLDSGQEMFLRRQQQHQQQQHQQQLQLLPHHLQHRPMAPKQKMGNPGSGGGEDVSAHQVQQAIIDQLFKAAELIDAGNPEHAHGILARLNHQISPMGKPFQRAAFYFKEAFQLLLQSNVNNSNNNSFSPTSLLLKIGAYKSFSEISPVLQFANFTSNQALLEAVEGFDRIHIIDFDIGFGGQWSSFMQELALRNGGAPALKITAFVSPSHHDEIELSFTNENLKQYAGEINMSFELEILTLESLNSVSWPQPLRDCEAVVVNLPICSFSNYPSYLPLVLRFVKQLMPKIVVTLDRSCDRTDAPVPQHMIFALQSYSGLLESLDAVSVNVHPDVLQMIEKYYLQPTIEKLVLGRLRSQDRTLPWKNLLLSSGFSPLTFSNFTESQAECLVQRIPGRGFHVEKKQNSLVLCWQRKDLISVSTWRC*