AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG036931

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036931 MtrunA17_Chr7g0231461 98.780 492 6 0 1 492 1 492 0.0 1008
MsaG036931 MtrunA17_Chr1g0193321 51.351 111 50 2 268 375 434 543 2.18e-24 107
MsaG036931 MtrunA17_Chr7g0262691 49.167 120 41 2 284 401 237 338 1.37e-23 103
MsaG036931 MtrunA17_Chr1g0182791 59.341 91 31 2 284 371 301 388 3.75e-23 102
MsaG036931 MtrunA17_Chr1g0153241 70.968 62 18 0 298 359 86 147 5.88e-23 98.2
MsaG036931 MtrunA17_Chr3g0095391 39.535 172 68 5 263 433 278 414 1.08e-22 100
MsaG036931 MtrunA17_Chr5g0402031 41.129 124 59 3 307 429 115 225 1.06e-18 87.4
MsaG036931 MtrunA17_Chr7g0271251 31.739 230 120 7 260 455 396 622 2.12e-18 88.6
MsaG036931 MtrunA17_Chr7g0270871 33.735 166 86 4 283 431 181 339 8.75e-17 82.0
MsaG036931 MtrunA17_Chr4g0074161 53.030 66 31 0 294 359 203 268 2.51e-15 77.8
MsaG036931 MtrunA17_Chr1g0190731 48.438 64 33 0 297 360 193 256 6.45e-13 70.1
MsaG036931 MtrunA17_Chr5g0421111 32.500 160 69 6 253 384 325 473 2.05e-12 69.3
MsaG036931 MtrunA17_Chr3g0144641 34.014 147 75 5 297 438 191 320 4.70e-12 67.8
MsaG036931 MtrunA17_Chr4g0035101 31.111 225 110 14 280 467 116 332 7.17e-12 66.6
MsaG036931 MtrunA17_Chr8g0365941 40.659 91 43 2 297 376 305 395 2.63e-11 65.9
MsaG036931 MtrunA17_Chr1g0152131 48.438 64 32 1 297 359 260 323 8.35e-11 63.9
MsaG036931 MtrunA17_Chr3g0134741 50.000 60 28 1 302 359 232 291 9.12e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG036931 AT4G00050.1 45.661 484 158 19 1 478 1 385 2.57e-102 312
MsaG036931 AT4G00050.3 44.583 480 159 20 1 472 1 381 2.45e-94 291
MsaG036931 AT4G00050.2 49.133 346 107 14 139 478 1 283 1.51e-76 242
MsaG036931 AT2G20180.4 29.047 451 233 16 1 434 1 381 2.11e-27 115
MsaG036931 AT2G20180.6 29.047 451 233 16 1 434 1 381 2.11e-27 115
MsaG036931 AT2G20180.2 29.047 451 233 16 1 434 1 381 2.11e-27 115
MsaG036931 AT2G20180.3 29.047 451 233 16 1 434 1 381 2.11e-27 115
MsaG036931 AT2G20180.8 29.973 377 207 14 1 358 1 339 4.13e-26 109
MsaG036931 AT2G20180.7 35.165 273 123 9 173 438 89 314 4.40e-26 110
MsaG036931 AT2G20180.1 35.165 273 123 9 173 438 89 314 4.40e-26 110
MsaG036931 AT2G20180.5 35.165 273 123 9 173 438 89 314 4.40e-26 110
MsaG036931 AT4G28790.1 30.496 282 171 8 112 389 101 361 1.62e-25 108
MsaG036931 AT4G36930.1 56.471 85 34 1 303 384 197 281 1.23e-22 99.8
MsaG036931 AT1G09530.3 45.082 122 57 1 270 391 310 421 1.67e-22 100
MsaG036931 AT1G09530.2 45.082 122 57 1 270 391 310 421 1.67e-22 100
MsaG036931 AT1G09530.5 45.082 122 57 1 270 391 310 421 1.67e-22 100
MsaG036931 AT1G09530.1 45.082 122 57 1 270 391 310 421 1.67e-22 100
MsaG036931 AT1G09530.4 45.082 122 57 1 270 391 310 421 1.67e-22 100
MsaG036931 AT1G09530.6 45.082 122 57 1 270 391 310 421 1.67e-22 100
MsaG036931 AT5G67110.1 57.471 87 34 1 287 370 77 163 1.71e-22 95.9
MsaG036931 AT4G28790.2 33.333 189 109 4 168 356 159 330 2.81e-21 95.5
MsaG036931 AT5G61270.2 36.232 207 126 4 281 484 56 259 1.79e-20 91.7
MsaG036931 AT5G61270.3 36.232 207 126 4 281 484 56 259 1.79e-20 91.7
MsaG036931 AT5G61270.1 36.232 207 126 4 281 484 144 347 3.01e-20 92.8
MsaG036931 AT2G46970.2 35.838 173 94 6 267 431 162 325 3.92e-20 92.8
MsaG036931 AT2G46970.1 35.838 173 94 6 267 431 193 356 5.01e-20 92.8
MsaG036931 AT2G46970.3 35.838 173 94 6 267 431 171 334 5.48e-20 92.4
MsaG036931 AT4G28815.2 58.209 67 28 0 291 357 62 128 1.57e-19 88.2
MsaG036931 AT4G28815.1 58.209 67 28 0 291 357 138 204 6.40e-19 87.8
MsaG036931 AT4G28800.1 38.095 168 68 5 280 446 236 368 1.29e-18 88.6
MsaG036931 AT3G62090.1 40.136 147 76 5 293 431 161 303 2.56e-18 86.7
MsaG036931 AT3G62090.2 40.136 147 76 5 293 431 178 320 2.70e-18 87.0
MsaG036931 AT4G28800.2 36.905 168 64 5 280 446 248 374 1.07e-17 85.9
MsaG036931 AT5G58010.1 52.941 68 32 0 293 360 95 162 3.83e-16 79.3
MsaG036931 AT2G24260.2 50.746 67 33 0 294 360 32 98 7.57e-16 77.8
MsaG036931 AT4G28811.1 30.405 296 171 9 194 488 265 526 7.85e-16 80.5
MsaG036931 AT4G30980.1 53.731 67 31 0 294 360 127 193 9.36e-16 78.6
MsaG036931 AT2G24260.1 50.746 67 33 0 294 360 135 201 1.66e-15 78.2
MsaG036931 AT3G59060.1 42.748 131 56 2 269 380 222 352 1.91e-15 79.0
MsaG036931 AT3G59060.3 42.748 131 56 2 269 380 222 352 2.00e-15 79.0
MsaG036931 AT3G59060.2 42.748 131 56 2 269 380 222 352 2.00e-15 79.0
MsaG036931 AT3G59060.4 42.748 131 56 2 269 380 222 352 2.00e-15 79.0
MsaG036931 AT4G30980.2 53.731 67 31 0 294 360 175 241 2.46e-15 77.8
MsaG036931 AT2G43010.4 46.078 102 48 1 298 392 252 353 3.01e-15 78.2
MsaG036931 AT2G43010.2 46.078 102 48 1 298 392 252 353 3.15e-15 78.2
MsaG036931 AT2G43010.1 46.078 102 48 1 298 392 252 353 3.81e-15 77.8
MsaG036931 AT2G43010.3 46.078 102 48 1 298 392 259 360 4.92e-15 77.8
MsaG036931 AT2G43010.5 46.078 102 48 1 298 392 294 395 6.79e-15 77.4
MsaG036931 AT1G02340.2 40.741 81 47 1 311 390 134 214 1.54e-11 65.5
MsaG036931 AT1G02340.1 33.871 124 59 3 311 433 141 242 1.80e-11 65.5
MsaG036931 AT5G50915.3 50.000 64 31 1 297 359 136 199 3.71e-11 64.3
MsaG036931 AT5G50915.4 50.000 64 31 1 297 359 136 199 3.71e-11 64.3
MsaG036931 AT1G27740.1 55.319 47 21 0 313 359 183 229 4.14e-11 63.9
MsaG036931 AT5G50915.1 50.000 64 31 1 297 359 136 199 4.34e-11 64.3
MsaG036931 AT5G50915.2 50.000 64 31 1 297 359 136 199 4.34e-11 64.3
MsaG036931 AT2G14760.1 47.619 63 31 1 299 359 237 299 5.10e-11 64.3
MsaG036931 AT1G68920.3 28.972 214 122 7 167 359 161 365 5.58e-11 65.1
MsaG036931 AT4G33880.1 47.619 63 31 1 299 359 266 328 7.83e-11 63.9

Find 189 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAATACCTTGCTGGAATT+TGG 0.116447 7:+48095835 None:intergenic
CTCGCTGGTGGTGGTGTTTA+TGG 0.210112 7:-48097606 MsaT036931.1:CDS
GATACGTACGATAGGGAATT+TGG 0.237818 7:-48097228 MsaT036931.1:CDS
TTCAACTTCAATAGCTTCTA+TGG 0.248590 7:-48097190 MsaT036931.1:CDS
CTAATACCTTGCTGGAATTT+GGG 0.269938 7:+48095836 None:intergenic
ATCCAACATTGAAGCTTTAT+CGG 0.300002 7:+48092804 None:intergenic
AATCATCAGTGTCCACTAAA+AGG 0.320838 7:-48096047 MsaT036931.1:CDS
AATCCAATGGGCATGGGAAT+GGG 0.328179 7:-48092664 MsaT036931.1:CDS
GGGTCAGCCATCCCGGCTGC+TGG 0.329557 7:+48092472 None:intergenic
ATTGCAAGCACAAGTGAATA+TGG 0.338807 7:-48092758 MsaT036931.1:CDS
TTTGCAATGTCTTCATTCTC+TGG 0.342653 7:+48095866 None:intergenic
CCAATGGGAATGGCCGGAAT+GGG 0.350269 7:-48092640 MsaT036931.1:CDS
AGGGTTAGTTGAATTAGAAT+TGG 0.352630 7:+48098177 None:intergenic
GCAGAATTGACATGGGAAAA+TGG 0.354104 7:-48097798 MsaT036931.1:CDS
CAAATTTCTATGCATGGCTT+AGG 0.354297 7:-48097774 MsaT036931.1:CDS
CACACGTGGCGGGGAGTGGC+AGG 0.362431 7:-48097308 MsaT036931.1:CDS
GTGGCACACGTGGCGGGGAG+TGG 0.363770 7:-48097312 MsaT036931.1:CDS
GGATGTTTATACGGCACTTT+CGG 0.364126 7:+48097747 None:intergenic
CATGATCCAATCCCTGATCT+AGG 0.366172 7:+48097438 None:intergenic
GGAGGGTGGCACACGTGGCG+GGG 0.367653 7:-48097317 MsaT036931.1:CDS
TCCCATACCCATCCCCATTC+CGG 0.371497 7:+48092627 None:intergenic
GGGAGGGTGGCACACGTGGC+GGG 0.375369 7:-48097318 MsaT036931.1:CDS
TATATTCTCTAATACCTTGC+TGG 0.384413 7:+48095828 None:intergenic
GCGGTTGATGGATGTTTATA+CGG 0.384417 7:+48097738 None:intergenic
GGCGGCTGTGGCAGCTGCGA+TGG 0.385766 7:+48097542 None:intergenic
TCTCTGCTCCTTAGTAGTAT+TGG 0.406917 7:+48097482 None:intergenic
GATGTTTATTTACCTGTGAT+TGG 0.409539 7:+48092428 None:intergenic
TGGGAGGGTGGCACACGTGG+CGG 0.410223 7:-48097319 MsaT036931.1:CDS
TGGAGAACCGGAGGCATCCC+AGG 0.410929 7:+48092559 None:intergenic
ACGTATCAAGTGTCACTTGC+TGG 0.412295 7:+48097244 None:intergenic
TTGCACTGCCACTCATACTT+TGG 0.415885 7:+48097283 None:intergenic
TATGGGAATGGGCATGGGAA+TGG 0.417658 7:-48092611 MsaT036931.1:CDS
ATGAGTGGCAGTGCAACCTT+TGG 0.418201 7:-48097276 MsaT036931.1:CDS
GCCAATGGGAATGGCCGGAA+TGG 0.418467 7:-48092641 MsaT036931.1:CDS
GGCCGGAATGGGGATGGGTA+TGG 0.418753 7:-48092629 MsaT036931.1:CDS
CAATTCTTTGAACCTGGTGC+TGG 0.423938 7:+48091485 None:intergenic
CATGCCGTTGTTGGCGGCTG+TGG 0.424655 7:+48097530 None:intergenic
TGCTGTCGTGGCTGCAACGC+AGG 0.424858 7:-48097379 MsaT036931.1:CDS
GGGCATGGGAATGGGGCCAA+TGG 0.424993 7:-48092656 MsaT036931.1:CDS
CAATGGGAATGGCCGGAATG+GGG 0.435164 7:-48092639 MsaT036931.1:CDS
AGGGATGTTAACGCGATTCA+TGG 0.435719 7:+48092579 None:intergenic
GAATCCAATGGGCATGGGAA+TGG 0.435727 7:-48092665 MsaT036931.1:CDS
ATGGGGATGGGTATGGGAAT+GGG 0.437973 7:-48092622 MsaT036931.1:CDS
CATTCATGCCTATGCCTTCG+TGG 0.438570 7:-48092528 MsaT036931.1:CDS
ACTGATCTTGGAGGTGGTTG+TGG 0.440551 7:+48098211 None:intergenic
CACCGCCATGCCGTTGTTGG+CGG 0.441882 7:+48097524 None:intergenic
ATCCACCGCCATGCCGTTGT+TGG 0.442498 7:+48097521 None:intergenic
ATGGATCCAGTCCCTAGATC+AGG 0.446722 7:-48097450 MsaT036931.1:CDS
GATGGGTATGGGAATGGGCA+TGG 0.448682 7:-48092617 MsaT036931.1:CDS
TGGGAAAAGCCTCGTGGTAG+TGG 0.449161 7:-48097690 MsaT036931.1:CDS
GGTGCACTGTTTCTCAGAGC+TGG 0.453141 7:+48097161 None:intergenic
CCCATTCCGGCCATTCCCAT+TGG 0.453787 7:+48092640 None:intergenic
AATGCAGATGGGTGGAGAAC+CGG 0.455967 7:+48092547 None:intergenic
CGTCAGCGGCAAAGCGTGGG+AGG 0.457321 7:-48097335 MsaT036931.1:CDS
GGGAATGGCCGGAATGGGGA+TGG 0.458260 7:-48092635 MsaT036931.1:CDS
AATGGGGATGGGTATGGGAA+TGG 0.459338 7:-48092623 MsaT036931.1:CDS
ATCGTCTACGGTGGTTGTCC+TGG 0.460526 7:+48097140 None:intergenic
ACACCACCACCAGCGAGAAA+TGG 0.461889 7:+48097612 None:intergenic
CGATGGCTGCAGCACGTTGT+GGG 0.463058 7:+48097559 None:intergenic
ATGGGTATGGGAATGGGCAT+GGG 0.465057 7:-48092616 MsaT036931.1:CDS
CTTCAAGGACCTCCAGCAGC+CGG 0.466012 7:-48092484 MsaT036931.1:CDS
GGGAATGGGGCCAATGGGAA+TGG 0.466616 7:-48092650 MsaT036931.1:CDS
GGCCCCATTCCCATGCCCAT+TGG 0.468409 7:+48092661 None:intergenic
TGAGCCAGTGTGTTCCTAGT+TGG 0.468451 7:-48098249 MsaT036931.1:CDS
AAATTTCTATGCATGGCTTA+GGG 0.469175 7:-48097773 MsaT036931.1:CDS
GAACGAAGAGAGACTGATCT+TGG 0.469694 7:+48098199 None:intergenic
TTCTTTGAACCTGGTGCTGG+AGG 0.470956 7:+48091488 None:intergenic
GCTGCGTCAGCGGCAAAGCG+TGG 0.471868 7:-48097339 MsaT036931.1:CDS
TTGTTGCATCTGCTGATACA+TGG 0.477496 7:+48091514 None:intergenic
GGTGTTGTACCTGCGGTTGA+TGG 0.477521 7:+48097726 None:intergenic
CGCCAACAACGGCATGGCGG+TGG 0.477769 7:-48097523 MsaT036931.1:CDS
ATGGGGCCAATGGGAATGGC+CGG 0.479464 7:-48092646 MsaT036931.1:CDS
AATGAAGACATTGCAAAAGT+TGG 0.480140 7:-48095860 MsaT036931.1:CDS
GGTGGTGGTGACCGACTTCA+AGG 0.482403 7:-48092499 MsaT036931.1:CDS
GCTCACTTACCCTTTCAGAT+TGG 0.484516 7:+48096001 None:intergenic
GCCGGAATGGGGATGGGTAT+GGG 0.484859 7:-48092628 MsaT036931.1:CDS
GATAGGGAATTTGGTATGAC+TGG 0.488691 7:-48097219 MsaT036931.1:CDS
GGCATGGGAATGGGGCCAAT+GGG 0.488847 7:-48092655 MsaT036931.1:CDS
GCACGTTGTGGGTCAAGCCA+TGG 0.490368 7:+48097570 None:intergenic
CTCCTTAGTAGTATTGGAGC+AGG 0.491917 7:+48097488 None:intergenic
ATGAATCGCGTTAACATCCC+TGG 0.495403 7:-48092577 MsaT036931.1:CDS
TGACCATGGATGCTTATAGT+AGG 0.495601 7:-48091544 MsaT036931.1:CDS
CCTATGCCTTCGTGGGATGC+CGG 0.500355 7:-48092520 MsaT036931.1:CDS
CGCAGGTACAACACCTTCCA+AGG 0.500491 7:-48097718 MsaT036931.1:CDS
CTGCGTCAGCGGCAAAGCGT+GGG 0.500962 7:-48097338 MsaT036931.1:CDS
AATGGACAAATTTCTATGCA+TGG 0.502362 7:-48097780 MsaT036931.1:CDS
GCGTGGGAGGGTGGCACACG+TGG 0.503424 7:-48097322 MsaT036931.1:CDS
CAACACCTTCCAAGGAAACA+TGG 0.504499 7:-48097710 MsaT036931.1:CDS
GGTTGGCGGCCACACACCTG+TGG 0.504726 7:-48097412 MsaT036931.1:CDS
TTGAAGTCGGTCACCACCAC+CGG 0.507200 7:+48092501 None:intergenic
GCCTCGTGGTAGTGGGACCT+TGG 0.508052 7:-48097682 MsaT036931.1:CDS
ATCCAATGGGCATGGGAATG+GGG 0.511377 7:-48092663 MsaT036931.1:CDS
CCTTCGTGGGATGCCGGTGG+TGG 0.512954 7:-48092514 MsaT036931.1:CDS
GCGATGGCTGCAGCACGTTG+TGG 0.514164 7:+48097558 None:intergenic
CACGTTGTGGGTCAAGCCAT+GGG 0.517113 7:+48097571 None:intergenic
GAGCCAGTGTGTTCCTAGTT+GGG 0.517262 7:-48098248 MsaT036931.1:CDS
TTCAAGGACCTCCAGCAGCC+GGG 0.524548 7:-48092483 MsaT036931.1:CDS
ACTATAAGCATCCATGGTCA+TGG 0.525290 7:+48091547 None:intergenic
CGGCATGGCGGTGGATGCGT+TGG 0.525524 7:-48097514 MsaT036931.1:CDS
ATGGAAACATGTTAGTCCCA+TGG 0.525747 7:-48097587 MsaT036931.1:CDS
TAACATCCCTGGGATGCCTC+CGG 0.526173 7:-48092566 MsaT036931.1:CDS
ACACGTGGCGGGGAGTGGCA+GGG 0.528731 7:-48097307 MsaT036931.1:CDS
CCAATCCCTGATCTAGGGAC+TGG 0.531824 7:+48097444 None:intergenic
CTGCTGGAGGTCCTTGAAGT+CGG 0.531831 7:+48092488 None:intergenic
TCAGCCATCCCGGCTGCTGG+AGG 0.532504 7:+48092475 None:intergenic
GGCATAGGCATGAATGCAGA+TGG 0.533264 7:+48092535 None:intergenic
AGGACCTCCAGCAGCCGGGA+TGG 0.533912 7:-48092479 MsaT036931.1:CDS
AGGCGTCCTTGCTGCGTCAG+CGG 0.534040 7:-48097349 MsaT036931.1:CDS
GCTGCCACAGCCGCCAACAA+CGG 0.534387 7:-48097534 MsaT036931.1:CDS
GGAATGGCCGGAATGGGGAT+GGG 0.536484 7:-48092634 MsaT036931.1:CDS
CCAGTCCCTAGATCAGGGAT+TGG 0.536661 7:-48097444 MsaT036931.1:CDS
CATTGTCATCGGCATCATCA+TGG 0.537813 7:+48092714 None:intergenic
TGAGTGGCAGTGCAACCTTT+GGG 0.538614 7:-48097275 MsaT036931.1:CDS
TCACCATTTCTCGCTGGTGG+TGG 0.540423 7:-48097615 MsaT036931.1:CDS
TATTGGAATTTCAGAAAAGG+AGG 0.542715 7:-48095900 MsaT036931.1:intron
TCCGCGGTGCGGAAAGCTAG+TGG 0.545630 7:+48097641 None:intergenic
GGGATTGGATCATGCATGGT+TGG 0.546571 7:-48097429 MsaT036931.1:CDS
TCCCTGCTCCAATACTACTA+AGG 0.550539 7:-48097490 MsaT036931.1:CDS
ATTGGAATTTCAGAAAAGGA+GGG 0.550647 7:-48095899 MsaT036931.1:intron
AATACACAACCAATCTGAAA+GGG 0.552633 7:-48096010 MsaT036931.1:intron
AGCGGCAAAGCGTGGGAGGG+TGG 0.553726 7:-48097331 MsaT036931.1:CDS
AACAGAATCATGATCGTCTA+CGG 0.554311 7:+48097128 None:intergenic
ATTGGATCATGCATGGTTGG+CGG 0.555215 7:-48097426 MsaT036931.1:CDS
TAGGGACTGGATCCATTGCA+TGG 0.557029 7:+48097457 None:intergenic
ATGTCGATGATGAATCCAAT+GGG 0.557625 7:-48092676 MsaT036931.1:CDS
GACCGATAAAGCTTCAATGT+TGG 0.557873 7:-48092806 MsaT036931.1:CDS
TTGCTGGCTCTGCCTCCCAA+AGG 0.562082 7:+48097260 None:intergenic
GGTGCGGAAAGCTAGTGGCT+TGG 0.564273 7:+48097646 None:intergenic
CTATAAGCATCCATGGTCAT+GGG 0.569472 7:+48091548 None:intergenic
TGACACTTGATACGTACGAT+AGG 0.571959 7:-48097236 MsaT036931.1:CDS
CGAAGAGAGACTGATCTTGG+AGG 0.573312 7:+48098202 None:intergenic
CCGGCATCCCACGAAGGCAT+AGG 0.573622 7:+48092520 None:intergenic
CACAGCCGCCAACAACGGCA+TGG 0.574856 7:-48097529 MsaT036931.1:CDS
GGAGAACCGGAGGCATCCCA+GGG 0.575931 7:+48092560 None:intergenic
ACAGATCAATTCTTTGAACC+TGG 0.576343 7:+48091479 None:intergenic
GGGATCTTGCAGTGCTGTCG+TGG 0.578008 7:-48097391 MsaT036931.1:CDS
ATGATCCAATCCCTGATCTA+GGG 0.578354 7:+48097439 None:intergenic
AGTTGTTGTTGCATTGTCAT+CGG 0.578530 7:+48092703 None:intergenic
GACACTTGATACGTACGATA+GGG 0.579795 7:-48097235 MsaT036931.1:CDS
TAAAGCTTCAATGTTGGATG+AGG 0.582737 7:-48092800 MsaT036931.1:CDS
AGAGAGACTGATCTTGGAGG+TGG 0.583200 7:+48098205 None:intergenic
CAACAACAGCCTCCAGCACC+AGG 0.583990 7:-48091497 MsaT036931.1:CDS
TGCAAGATCCCACAGGTGTG+TGG 0.585225 7:+48097403 None:intergenic
AATGTCGATGATGAATCCAA+TGG 0.587525 7:-48092677 MsaT036931.1:CDS
GCTCTGAGAAACAGTGCACC+AGG 0.588338 7:-48097158 MsaT036931.1:CDS
AATCCTACTATAAGCATCCA+TGG 0.589299 7:+48091541 None:intergenic
GGGAAAAGCCTCGTGGTAGT+GGG 0.593722 7:-48097689 MsaT036931.1:CDS
TTGGTTCACTATAGACTCCA+AGG 0.593852 7:+48097665 None:intergenic
TGAATCGCGTTAACATCCCT+GGG 0.595225 7:-48092576 MsaT036931.1:CDS
GCTTTGCCGCTGACGCAGCA+AGG 0.596395 7:+48097343 None:intergenic
GATGATGAATCCAATGGGCA+TGG 0.598687 7:-48092671 MsaT036931.1:CDS
CAATACACAACCAATCTGAA+AGG 0.601190 7:-48096011 MsaT036931.1:CDS
GGAAAATCACCATTTCTCGC+TGG 0.601319 7:-48097621 MsaT036931.1:CDS
ATGATGAATCCAATGGGCAT+GGG 0.601337 7:-48092670 MsaT036931.1:CDS
AAATCACCATTTCTCGCTGG+TGG 0.608017 7:-48097618 MsaT036931.1:CDS
TGTTTGTCACAGTAGACCAC+CGG 0.608502 7:-48097109 MsaT036931.1:intron
ATCAGGGATTGGATCATGCA+TGG 0.608506 7:-48097433 MsaT036931.1:CDS
ATTCATGCCTATGCCTTCGT+GGG 0.610276 7:-48092527 MsaT036931.1:CDS
TGGATCCAGTCCCTAGATCA+GGG 0.617422 7:-48097449 MsaT036931.1:CDS
AGGGACTGGATCCATTGCAT+GGG 0.619973 7:+48097458 None:intergenic
GCTGCAACGCAGGACGAAGG+AGG 0.620227 7:-48097369 MsaT036931.1:CDS
GTCAGCGGCAAAGCGTGGGA+GGG 0.620551 7:-48097334 MsaT036931.1:CDS
AACACCTTCCAAGGAAACAT+GGG 0.622242 7:-48097709 MsaT036931.1:CDS
GGCAGGGACCAAAGTATGAG+TGG 0.622617 7:-48097291 MsaT036931.1:CDS
GTGGCTGCAACGCAGGACGA+AGG 0.623187 7:-48097372 MsaT036931.1:CDS
GTTGGCGGCCACACACCTGT+GGG 0.625793 7:-48097411 MsaT036931.1:CDS
GCTGACATGGGAACATCATG+AGG 0.626938 7:+48098157 None:intergenic
GGAGCAGAGAACCCATGCAA+TGG 0.627174 7:-48097469 MsaT036931.1:CDS
ATAGAGGGAGGAAGCTGACG+TGG 0.633549 7:-48096094 MsaT036931.1:intron
ATGCCTTCGTGGGATGCCGG+TGG 0.634760 7:-48092517 MsaT036931.1:CDS
ATGATGTTGCAGAATTGACA+TGG 0.641179 7:-48097806 MsaT036931.1:CDS
ACAGCACTGCAAGATCCCAC+AGG 0.641870 7:+48097396 None:intergenic
TATAAACATCCATCAACCGC+AGG 0.646938 7:-48097735 MsaT036931.1:CDS
TAGGCATGAATGCAGATGGG+TGG 0.647258 7:+48092539 None:intergenic
GCATAGGCATGAATGCAGAT+GGG 0.648584 7:+48092536 None:intergenic
CTGACATGGGAACATCATGA+GGG 0.655129 7:+48098158 None:intergenic
GCAGATGGGTGGAGAACCGG+AGG 0.655471 7:+48092550 None:intergenic
TCCTTAGTAGTATTGGAGCA+GGG 0.655762 7:+48097489 None:intergenic
CTTGGAAGGTGTTGTACCTG+CGG 0.658125 7:+48097719 None:intergenic
GCCACTAGCTTTCCGCACCG+CGG 0.661460 7:-48097642 MsaT036931.1:CDS
GTGGCAGTGCAACCTTTGGG+AGG 0.676643 7:-48097272 MsaT036931.1:CDS
GTTGGTCCCAAATTCCAGCA+AGG 0.680499 7:-48095842 MsaT036931.1:intron
CCACCACCGGCATCCCACGA+AGG 0.687100 7:+48092514 None:intergenic
CATCCCAACTAGGAACACAC+TGG 0.691416 7:+48098245 None:intergenic
AGCCGCCAACAACGGCATGG+CGG 0.704952 7:-48097526 MsaT036931.1:CDS
AGAATCATGATCGTCTACGG+TGG 0.711312 7:+48097131 None:intergenic
TCCAAGGTCCCACTACCACG+AGG 0.721374 7:+48097681 None:intergenic
TGATGTTGCAGAATTGACAT+GGG 0.723669 7:-48097805 MsaT036931.1:CDS
GAAACATGGGAAAAGCCTCG+TGG 0.735368 7:-48097696 MsaT036931.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 48091484 48098272 48091484 ID=MsaG036931
Chr7 mRNA 48091484 48098272 48091484 ID=MsaT036931.1;Parent=MsaG036931
Chr7 exon 48091484 48091570 48091484 ID=MsaT036931.1.exon6;Parent=MsaT036931.1
Chr7 CDS 48091484 48091570 48091484 ID=cds.MsaT036931.1;Parent=MsaT036931.1
Chr7 exon 48092441 48092827 48092441 ID=MsaT036931.1.exon5;Parent=MsaT036931.1
Chr7 CDS 48092441 48092827 48092441 ID=cds.MsaT036931.1;Parent=MsaT036931.1
Chr7 exon 48095843 48095908 48095843 ID=MsaT036931.1.exon4;Parent=MsaT036931.1
Chr7 CDS 48095843 48095908 48095843 ID=cds.MsaT036931.1;Parent=MsaT036931.1
Chr7 exon 48096011 48096112 48096011 ID=MsaT036931.1.exon3;Parent=MsaT036931.1
Chr7 CDS 48096011 48096112 48096011 ID=cds.MsaT036931.1;Parent=MsaT036931.1
Chr7 exon 48097110 48097836 48097110 ID=MsaT036931.1.exon2;Parent=MsaT036931.1
Chr7 CDS 48097110 48097836 48097110 ID=cds.MsaT036931.1;Parent=MsaT036931.1
Chr7 exon 48098163 48098272 48098163 ID=MsaT036931.1.exon1;Parent=MsaT036931.1
Chr7 CDS 48098163 48098272 48098163 ID=cds.MsaT036931.1;Parent=MsaT036931.1
Gene Sequence

>MsaT036931.1

ATGAGCCAGTGTGTTCCTAGTTGGGATGTTGACGAAAATCCACAACCACCTCCAAGATCAGTCTCTCTTCGTTCCAATTCTAATTCAACTAACCCTCATGATGTTCCCATGTTAGACTATGATGTTGCAGAATTGACATGGGAAAATGGACAAATTTCTATGCATGGCTTAGGGTTACCGAAAGTGCCGTATAAACATCCATCAACCGCAGGTACAACACCTTCCAAGGAAACATGGGAAAAGCCTCGTGGTAGTGGGACCTTGGAGTCTATAGTGAACCAAGCCACTAGCTTTCCGCACCGCGGAAAATCACCATTTCTCGCTGGTGGTGGTGTTTATGGAAACATGTTAGTCCCATGGCTTGACCCACAACGTGCTGCAGCCATCGCAGCTGCCACAGCCGCCAACAACGGCATGGCGGTGGATGCGTTGGTTCCCTGCTCCAATACTACTAAGGAGCAGAGAACCCATGCAATGGATCCAGTCCCTAGATCAGGGATTGGATCATGCATGGTTGGCGGCCACACACCTGTGGGATCTTGCAGTGCTGTCGTGGCTGCAACGCAGGACGAAGGAGGCGTCCTTGCTGCGTCAGCGGCAAAGCGTGGGAGGGTGGCACACGTGGCGGGGAGTGGCAGGGACCAAAGTATGAGTGGCAGTGCAACCTTTGGGAGGCAGAGCCAGCAAGTGACACTTGATACGTACGATAGGGAATTTGGTATGACTGGTTTCACTTCAACTTCAATAGCTTCTATGGATAACACCAGCTCTGAGAAACAGTGCACCAGGACAACCACCGTAGACGATCATGATTCTGTTTGTCACAGTAGACCACCGAGGGAGGAAGCTGACGTGGATGAGAAAAAAAGAGAAAATAGAAAATCATCAGTGTCCACTAAAAGGAGTAGAGCTGCAGCAATACACAACCAATCTGAAAGGAAAAGGAGGGATAAGATAAACCAGAGAATGAAGACATTGCAAAAGTTGGTCCCAAATTCCAGCAAGACCGATAAAGCTTCAATGTTGGATGAGGTGATAGAATATCTGAAACAATTGCAAGCACAAGTGAATATGGTGAATAGATTCAACATGTCATCCATGATGATGCCGATGACAATGCAACAACAACTTCAAATGTCGATGATGAATCCAATGGGCATGGGAATGGGGCCAATGGGAATGGCCGGAATGGGGATGGGTATGGGAATGGGCATGGGAATGGATATGAACACCATGAATCGCGTTAACATCCCTGGGATGCCTCCGGTTCTCCACCCATCTGCATTCATGCCTATGCCTTCGTGGGATGCCGGTGGTGGTGACCGACTTCAAGGACCTCCAGCAGCCGGGATGGCTGACCCCCTCTCCACATTTTTTGGTTGCCAATCACAGCCCATGACCATGGATGCTTATAGTAGGATTGCAGCCATGTATCAGCAGATGCAACAACAGCCTCCAGCACCAGGTTCAAAGAATTGA
Protein sequence

>MsaT036931.1

MSQCVPSWDVDENPQPPPRSVSLRSNSNSTNPHDVPMLDYDVAELTWENGQISMHGLGLPKVPYKHPSTAGTTPSKETWEKPRGSGTLESIVNQATSFPHRGKSPFLAGGGVYGNMLVPWLDPQRAAAIAAATAANNGMAVDALVPCSNTTKEQRTHAMDPVPRSGIGSCMVGGHTPVGSCSAVVAATQDEGGVLAASAAKRGRVAHVAGSGRDQSMSGSATFGRQSQQVTLDTYDREFGMTGFTSTSIASMDNTSSEKQCTRTTTVDDHDSVCHSRPPREEADVDEKKRENRKSSVSTKRSRAAAIHNQSERKRRDKINQRMKTLQKLVPNSSKTDKASMLDEVIEYLKQLQAQVNMVNRFNMSSMMMPMTMQQQLQMSMMNPMGMGMGPMGMAGMGMGMGMGMGMDMNTMNRVNIPGMPPVLHPSAFMPMPSWDAGGGDRLQGPPAAGMADPLSTFFGCQSQPMTMDAYSRIAAMYQQMQQQPPAPGSKN*