AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037021

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037021 MtrunA17_Chr7g0232671 87.891 256 26 2 1 255 1 252 7.01e-166 458
MsaG037021 MtrunA17_Chr7g0232681 47.458 236 51 7 24 254 29 196 3.52e-55 176
MsaG037021 MtrunA17_Chr7g0232701 75.269 93 21 1 162 254 47 137 3.55e-43 143
MsaG037021 MtrunA17_Chr3g0140331 66.102 59 20 0 190 248 253 311 7.31e-22 93.2
MsaG037021 MtrunA17_Chr4g0028961 45.794 107 54 2 149 251 187 293 1.31e-21 91.7
MsaG037021 MtrunA17_Chr4g0035891 53.333 75 35 0 177 251 198 272 1.44e-21 91.3
MsaG037021 MtrunA17_Chr1g0146231 62.903 62 23 0 190 251 234 295 3.55e-21 90.9
MsaG037021 MtrunA17_Chr3g0145121 58.730 63 26 0 189 251 214 276 3.91e-21 90.1
MsaG037021 MtrunA17_Chr1g0199671 62.903 62 23 0 190 251 242 303 6.76e-21 90.1
MsaG037021 MtrunA17_Chr2g0326771 46.067 89 48 0 167 255 193 281 7.68e-21 89.7
MsaG037021 MtrunA17_Chr6g0461591 61.290 62 24 0 190 251 192 253 8.42e-21 88.6
MsaG037021 MtrunA17_Chr1g0213161 61.290 62 24 0 190 251 152 213 9.82e-21 87.8
MsaG037021 MtrunA17_Chr5g0404981 43.000 100 54 1 152 251 265 361 1.29e-20 89.7
MsaG037021 MtrunA17_Chr8g0340401 61.290 62 24 0 190 251 226 287 1.74e-20 89.0
MsaG037021 MtrunA17_Chr7g0253101 59.677 62 25 0 190 251 188 249 2.62e-20 87.4
MsaG037021 MtrunA17_Chr8g0372291 58.065 62 26 0 190 251 218 279 7.24e-20 86.7
MsaG037021 MtrunA17_Chr1g0169431 46.809 94 44 2 163 254 149 238 2.81e-19 84.0
MsaG037021 MtrunA17_Chr5g0395391 56.716 67 27 1 190 254 270 336 2.44e-18 83.2
MsaG037021 MtrunA17_Chr5g0395391 56.452 62 27 0 190 251 168 229 3.55e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037021 AT3G60530.1 54.930 71 32 0 181 251 149 219 8.27e-23 94.0
MsaG037021 AT5G25830.1 61.905 63 24 0 189 251 218 280 2.04e-22 94.4
MsaG037021 AT4G32890.1 61.290 62 24 0 190 251 197 258 7.81e-22 92.4
MsaG037021 AT3G45170.1 57.143 63 27 0 189 251 114 176 1.30e-21 89.7
MsaG037021 AT4G36240.1 61.290 62 24 0 190 251 164 225 1.74e-21 90.1
MsaG037021 AT2G45050.1 58.065 62 26 0 190 251 179 240 2.71e-21 90.1
MsaG037021 AT3G54810.1 60.606 66 26 0 183 248 222 287 3.66e-21 90.9
MsaG037021 AT3G54810.2 60.606 66 26 0 183 248 222 287 3.66e-21 90.9
MsaG037021 AT1G08000.2 48.454 97 45 2 155 251 188 279 3.69e-21 90.9
MsaG037021 AT1G08000.1 48.454 97 45 2 155 251 188 279 3.69e-21 90.9
MsaG037021 AT2G28340.1 61.667 60 23 0 192 251 193 252 6.46e-21 89.7
MsaG037021 AT1G08010.2 47.423 97 46 2 155 251 190 281 8.07e-21 89.7
MsaG037021 AT1G08010.1 47.423 97 46 2 155 251 190 281 8.07e-21 89.7
MsaG037021 AT1G08010.3 47.423 97 46 2 155 251 190 281 8.07e-21 89.7
MsaG037021 AT5G66320.2 57.143 63 27 0 189 251 248 310 1.20e-20 89.7
MsaG037021 AT5G66320.1 57.143 63 27 0 189 251 248 310 1.20e-20 89.7
MsaG037021 AT4G34680.1 50.000 76 37 1 176 251 167 241 1.41e-20 88.6
MsaG037021 AT4G34680.2 50.000 76 37 1 176 251 167 241 1.41e-20 88.6
MsaG037021 AT3G51080.1 58.065 62 26 0 190 251 221 282 6.25e-20 87.4
MsaG037021 AT3G24050.1 56.716 67 27 1 190 254 194 260 2.01e-19 85.5

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTCTCTCTCATAAATATT+TGG 0.165198 7:-50310392 None:intergenic
TGAGAGAGAAATTATCAATT+TGG 0.205113 7:+50310403 MsaT037021.1:CDS
AAAGGAGGTGCTACAAAATT+TGG 0.217622 7:+50310166 MsaT037021.1:CDS
GAATATACCAAAAGAGAAAT+TGG 0.273307 7:+50310499 MsaT037021.1:CDS
AAAGGTCAAATTCAATCAAT+TGG 0.302205 7:+50310277 MsaT037021.1:CDS
ATTGTATCCAATTTCTCTTT+TGG 0.309899 7:-50310506 None:intergenic
TCTCCATTGAGTAGTGTATT+TGG 0.321114 7:-50310678 None:intergenic
TGGTATATTCTCTTGATTGA+TGG 0.325827 7:-50310486 None:intergenic
TTCACTTTAGTCCTTCTTAG+AGG 0.336639 7:-50310581 None:intergenic
CAATGGAGAATGGGTCCTTT+GGG 0.340295 7:+50310692 MsaT037021.1:CDS
TCAATGGAGAATGGGTCCTT+TGG 0.360479 7:+50310691 MsaT037021.1:CDS
TACCATGGAGAAAAGTTTGC+AGG 0.430974 7:+50310211 MsaT037021.1:CDS
TCATAAGATCTTGTTGAGAA+AGG 0.455470 7:+50310826 MsaT037021.1:CDS
AAAGAGAAATTGGATACAAT+AGG 0.462489 7:+50310509 MsaT037021.1:CDS
TACACTACTCAATGGAGAAT+GGG 0.464174 7:+50310683 MsaT037021.1:CDS
AAGGTCAAATTCAATCAATT+GGG 0.464264 7:+50310278 MsaT037021.1:CDS
AATATGAAAGAAGAGCAATA+TGG 0.475297 7:+50310539 MsaT037021.1:CDS
AAGTCAAATCGATTGGTTCA+AGG 0.491839 7:+50310752 MsaT037021.1:CDS
AGTCAAATCGATTGGTTCAA+GGG 0.501447 7:+50310753 MsaT037021.1:CDS
ATACACTACTCAATGGAGAA+TGG 0.502570 7:+50310682 MsaT037021.1:CDS
TGACACTTGTGAGAAAGAAA+AGG 0.507889 7:+50310259 MsaT037021.1:CDS
AAGAATAAAATTGCTCTGCA+TGG 0.509608 7:-50310136 None:intergenic
AAAGAAGAGCAATATGGTAA+AGG 0.511666 7:+50310545 MsaT037021.1:CDS
TGAATGCCTATTAAAATCAA+AGG 0.520274 7:-50310798 None:intergenic
ACGATACAAGTCAAATCGAT+TGG 0.539842 7:+50310745 MsaT037021.1:CDS
AAACTCAAAAGCCTCTAAGA+AGG 0.540850 7:+50310570 MsaT037021.1:CDS
AGACCAAATACACTACTCAA+TGG 0.550163 7:+50310675 MsaT037021.1:CDS
AAGATCTTGTTGAGAAAGGA+AGG 0.551664 7:+50310830 MsaT037021.1:CDS
TCAAAGGATGGACTTGCCGC+AGG 0.556754 7:-50310782 None:intergenic
TGCCTATTAAAATCAAAGGA+TGG 0.573342 7:-50310794 None:intergenic
AAATTCAATCAATTGGGTGA+AGG 0.606199 7:+50310284 MsaT037021.1:CDS
CTCGAAAATTAAATATCATG+AGG 0.610888 7:+50310109 MsaT037021.1:CDS
CATGGAGAAAAGTTTGCAGG+AGG 0.621981 7:+50310214 MsaT037021.1:CDS
AATGGAGAATGGGTCCTTTG+GGG 0.623322 7:+50310693 MsaT037021.1:CDS
AATACATTGTGCAATGCATG+TGG 0.624464 7:+50310719 MsaT037021.1:CDS
TTATTCTTATATCCCAAAGG+AGG 0.626923 7:+50310151 MsaT037021.1:CDS
GTTTGTCTAATGATTTCGTG+TGG 0.672848 7:+50310369 MsaT037021.1:CDS
GGTTCAAGGGTATAGACCTG+CGG 0.691775 7:+50310766 MsaT037021.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50310089 50310856 50310089 ID=MsaG037021
Chr7 mRNA 50310089 50310856 50310089 ID=MsaT037021.1;Parent=MsaG037021
Chr7 exon 50310089 50310856 50310089 ID=MsaT037021.1.exon1;Parent=MsaT037021.1
Chr7 CDS 50310089 50310856 50310089 ID=cds.MsaT037021.1;Parent=MsaT037021.1
Gene Sequence

>MsaT037021.1

ATGGCTATATTCGACTTCAACTCGAAAATTAAATATCATGAGGAAAACCATGCAGAGCAATTTTATTCTTATATCCCAAAGGAGGTGCTACAAAATTTGGAAAGCTACCCTACTTTTTGGGATACCATGGAGAAAAGTTTGCAGGAGGATTTGAATGATTTTCTTTTTTGTGACACTTGTGAGAAAGAAAAGGTCAAATTCAATCAATTGGGTGAAGGTAAAACACAATCTTTCGATGTAACAAATGTTCAAATATATCAAGAGCACAATATTGACACATGTTTGTCTAATGATTTCGTGTGGCCAAATATTTATGAGAGAGAAATTATCAATTTGGAGCAACCTAGTGAAGAACAAAACCTAAAAGTTCCTTTTTTGGATGTGTCAAATATTCATGCCATCAATCAAGAGAATATACCAAAAGAGAAATTGGATACAATAGGTAAAGAGAATATGAAAGAAGAGCAATATGGTAAAGGAAAAACTCAAAAGCCTCTAAGAAGGACTAAAGTGAAGTCAAGTTCTATAAAGAAGAGTAGAGCAAAGAGCATAGATATGTATAGAGAGAGAAGTTGCAGTCATTGTGAGACCAAATACACTACTCAATGGAGAATGGGTCCTTTGGGGAAAAATACATTGTGCAATGCATGTGGATTACGATACAAGTCAAATCGATTGGTTCAAGGGTATAGACCTGCGGCAAGTCCATCCTTTGATTTTAATAGGCATTCAAATTTTCATAAGATCTTGTTGAGAAAGGAAGGATAA
Protein sequence

>MsaT037021.1

MAIFDFNSKIKYHEENHAEQFYSYIPKEVLQNLESYPTFWDTMEKSLQEDLNDFLFCDTCEKEKVKFNQLGEGKTQSFDVTNVQIYQEHNIDTCLSNDFVWPNIYEREIINLEQPSEEQNLKVPFLDVSNIHAINQENIPKEKLDTIGKENMKEEQYGKGKTQKPLRRTKVKSSSIKKSRAKSIDMYRERSCSHCETKYTTQWRMGPLGKNTLCNACGLRYKSNRLVQGYRPAASPSFDFNRHSNFHKILLRKEG*