AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037023


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037023 MtrunA17_Chr7g0232681 82.653 196 24 1 1 186 1 196 2.87e-117 330
MsaG037023 MtrunA17_Chr7g0232701 75.168 149 15 2 48 186 1 137 1.60e-73 218
MsaG037023 MtrunA17_Chr7g0232671 43.162 234 53 6 29 187 24 252 1.80e-49 160
MsaG037023 MtrunA17_Chr3g0140331 54.762 84 37 1 98 180 228 311 3.53e-22 92.0
MsaG037023 MtrunA17_Chr2g0326771 35.461 141 75 3 55 187 149 281 1.38e-19 84.3
MsaG037023 MtrunA17_Chr4g0028961 55.385 65 29 0 119 183 229 293 1.62e-19 84.0
MsaG037023 MtrunA17_Chr4g0035891 32.911 158 100 3 30 183 117 272 2.04e-19 83.6
MsaG037023 MtrunA17_Chr7g0253101 55.385 65 29 0 119 183 185 249 1.06e-18 80.9
MsaG037023 MtrunA17_Chr3g0145121 53.731 67 29 1 121 185 214 280 1.19e-18 81.6
MsaG037023 MtrunA17_Chr1g0199671 56.452 62 27 0 122 183 242 303 1.85e-18 81.3
MsaG037023 MtrunA17_Chr1g0146231 56.452 62 27 0 122 183 234 295 2.60e-18 80.9
MsaG037023 MtrunA17_Chr1g0213161 39.286 112 66 2 74 183 102 213 5.55e-18 78.6
MsaG037023 MtrunA17_Chr6g0461591 54.839 62 28 0 122 183 192 253 6.27e-18 79.0
MsaG037023 MtrunA17_Chr8g0340401 54.839 62 28 0 122 183 226 287 1.23e-17 79.0
MsaG037023 MtrunA17_Chr5g0404981 53.226 62 29 0 122 183 300 361 4.17e-17 78.2
MsaG037023 MtrunA17_Chr8g0372291 53.226 62 29 0 122 183 218 279 4.76e-17 77.0
MsaG037023 MtrunA17_Chr1g0169431 53.731 67 29 1 122 186 172 238 1.87e-16 74.7
MsaG037023 MtrunA17_Chr5g0395391 33.858 127 80 1 61 183 205 331 9.26e-16 73.9
MsaG037023 MtrunA17_Chr5g0395391 50.000 62 31 0 122 183 168 229 7.13e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037023 AT4G34680.1 32.317 164 106 1 20 183 83 241 1.00e-22 92.4
MsaG037023 AT4G34680.2 32.317 164 106 1 20 183 83 241 1.00e-22 92.4
MsaG037023 AT3G60530.1 56.250 64 28 0 120 183 156 219 3.63e-21 87.4
MsaG037023 AT5G25830.1 58.730 63 26 0 121 183 218 280 2.10e-20 87.0
MsaG037023 AT2G45050.1 54.688 64 29 0 120 183 177 240 3.21e-20 85.5
MsaG037023 AT3G45170.1 31.544 149 97 2 37 183 31 176 5.81e-20 83.6
MsaG037023 AT4G32890.1 42.574 101 46 2 95 183 158 258 7.33e-20 85.1
MsaG037023 AT3G54810.1 60.000 65 24 1 122 184 229 293 4.69e-19 83.2
MsaG037023 AT3G54810.2 60.000 65 24 1 122 184 229 293 4.69e-19 83.2
MsaG037023 AT2G28340.1 58.333 60 25 0 124 183 193 252 1.83e-18 81.3
MsaG037023 AT4G36240.1 53.226 62 29 0 122 183 164 225 2.21e-18 80.1
MsaG037023 AT5G66320.2 43.956 91 44 1 100 183 220 310 3.21e-18 81.3
MsaG037023 AT5G66320.1 43.956 91 44 1 100 183 220 310 3.21e-18 81.3
MsaG037023 AT3G24050.1 51.351 74 34 1 115 186 187 260 9.21e-18 79.0
MsaG037023 AT1G08000.2 55.224 67 28 1 122 186 218 284 2.30e-17 78.6
MsaG037023 AT1G08000.1 55.224 67 28 1 122 186 218 284 2.30e-17 78.6
MsaG037023 AT1G08010.2 55.932 59 26 0 122 180 220 278 2.35e-17 78.2
MsaG037023 AT1G08010.1 55.932 59 26 0 122 180 220 278 2.35e-17 78.2
MsaG037023 AT1G08010.3 55.932 59 26 0 122 180 220 278 2.35e-17 78.2
MsaG037023 AT3G51080.1 53.226 62 29 0 122 183 221 282 4.57e-17 77.8

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGAGGCACTAGAAAATT+TGG 0.257280 7:+50318162 MsaT037023.1:CDS
AAGCTAAATGGATTGGTTAA+AGG 0.267058 7:+50318529 MsaT037023.1:CDS
TCAATGGAGGTTGGGTCCTT+TGG 0.284044 7:+50318468 MsaT037023.1:CDS
GTACTGGCAATCCTCATCTT+TGG 0.297320 7:+50317693 MsaT037023.1:CDS
CCTCCATTGAGTAGTTTCTT+TGG 0.310305 7:-50318455 None:intergenic
GGATTACGATACAAGCTAAA+TGG 0.317668 7:+50318517 MsaT037023.1:CDS
AGCTAAATGGATTGGTTAAA+GGG 0.347180 7:+50318530 MsaT037023.1:CDS
TACTGGCAATCCTCATCTTT+GGG 0.354229 7:+50317694 MsaT037023.1:CDS
CAATGGAGGTTGGGTCCTTT+GGG 0.359077 7:+50318469 MsaT037023.1:CDS
CAAATTCTCTGATGATGATT+AGG 0.374222 7:-50318337 None:intergenic
ACTAGAGCCAAGACATTCAT+AGG 0.414135 7:+50318406 MsaT037023.1:CDS
CCGCCTTGTGTAAATTTGAA+TGG 0.415356 7:-50318591 None:intergenic
TCAAAAGCTGGACTTGCCTT+AGG 0.449589 7:-50318559 None:intergenic
AAATGTTCAAAATAATCAAC+AGG 0.466273 7:+50318294 MsaT037023.1:CDS
ACATTTGACACATTGAAAGA+TGG 0.475716 7:-50318277 None:intergenic
TCTCTCTCCTATGAATGTCT+TGG 0.487683 7:-50318413 None:intergenic
GAAACTACTCAATGGAGGTT+GGG 0.488791 7:+50318460 MsaT037023.1:CDS
AACATGTAGTGGGTCAGTAC+TGG 0.492576 7:+50317677 MsaT037023.1:CDS
ACGATACAAGCTAAATGGAT+TGG 0.515501 7:+50318522 MsaT037023.1:CDS
AGAAACTACTCAATGGAGGT+TGG 0.521850 7:+50318459 MsaT037023.1:CDS
ACACAAGGCGGCGATGAGAA+AGG 0.533727 7:+50318603 MsaT037023.1:CDS
GGTTAAAGGGTATAGACCTA+AGG 0.562082 7:+50318543 MsaT037023.1:CDS
TGGTTTCTGATATCAAAAGC+TGG 0.565390 7:-50318571 None:intergenic
AGACCAAAGAAACTACTCAA+TGG 0.572896 7:+50318452 MsaT037023.1:CDS
AGATTTGAGTAAACATGTAG+TGG 0.573609 7:+50317666 MsaT037023.1:CDS
CATCAAATTGGATCAATCGA+TGG 0.580669 7:+50318231 MsaT037023.1:CDS
TAGTTTCTTTGGTCTCACAA+TGG 0.599313 7:-50318444 None:intergenic
AATACTTTGTGCAATGCATG+TGG 0.610080 7:+50318496 MsaT037023.1:CDS
AAGGCGGCGATGAGAAAGGA+AGG 0.631884 7:+50318607 MsaT037023.1:CDS
ATAATACTAACCCAAAGATG+AGG 0.642509 7:-50317704 None:intergenic
GATTTGAGTAAACATGTAGT+GGG 0.645437 7:+50317667 MsaT037023.1:CDS
CCAAAGAAACTACTCAATGG+AGG 0.662722 7:+50318455 MsaT037023.1:CDS
CCATTCAAATTTACACAAGG+CGG 0.663290 7:+50318591 MsaT037023.1:CDS
TTGGCTCTAGTCCTTCGTAG+AGG 0.667318 7:-50318394 None:intergenic
CTACTCAAAAGCCTCTACGA+AGG 0.678918 7:+50318383 MsaT037023.1:CDS
ACTTGATTATATCCCACATG+AGG 0.689620 7:+50318147 MsaT037023.1:CDS
AAACCATTCAAATTTACACA+AGG 0.692002 7:+50318588 MsaT037023.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50317643 50318633 50317643 ID=MsaG037023
Chr7 mRNA 50317643 50318633 50317643 ID=MsaT037023.1;Parent=MsaG037023
Chr7 exon 50317643 50317715 50317643 ID=MsaT037023.1.exon1;Parent=MsaT037023.1
Chr7 CDS 50317643 50317715 50317643 ID=cds.MsaT037023.1;Parent=MsaT037023.1
Chr7 exon 50318143 50318633 50318143 ID=MsaT037023.1.exon2;Parent=MsaT037023.1
Chr7 CDS 50318143 50318633 50318143 ID=cds.MsaT037023.1;Parent=MsaT037023.1
Gene Sequence

>MsaT037023.1

ATGGCTAATCTGAGAGAAGAACAAGATTTGAGTAAACATGTAGTGGGTCAGTACTGGCAATCCTCATCTTTGGGGAAACTTGATTATATCCCACATGAGGCACTAGAAAATTTGGAATGCTACACTACTTTTTGGGATGACATGAAGAAATTAGAAAAAGTCATCAAATTGGATCAATCGATGGATGAGAGAAAAACACAAGAAAATCCATCTTTCAATGTGTCAAATGTTCAAAATAATCAACAGGAGAATATTGATACAAGTGTGCCTAATCATCATCAGAGAATTTGCAGCGATAGTGAGACCAAAGCAACTACTCAAAAGCCTCTACGAAGGACTAGAGCCAAGACATTCATAGGAGAGAGAAGTTGCAGCCATTGTGAGACCAAAGAAACTACTCAATGGAGGTTGGGTCCTTTGGGAAAAAATACTTTGTGCAATGCATGTGGATTACGATACAAGCTAAATGGATTGGTTAAAGGGTATAGACCTAAGGCAAGTCCAGCTTTTGATATCAGAAACCATTCAAATTTACACAAGGCGGCGATGAGAAAGGAAGGATAA

Protein sequence

>MsaT037023.1

MANLREEQDLSKHVVGQYWQSSSLGKLDYIPHEALENLECYTTFWDDMKKLEKVIKLDQSMDERKTQENPSFNVSNVQNNQQENIDTSVPNHHQRICSDSETKATTQKPLRRTRAKTFIGERSCSHCETKETTQWRLGPLGKNTLCNACGLRYKLNGLVKGYRPKASPAFDIRNHSNLHKAAMRKEG*