AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037030 MtrunA17_Chr7g0232811 88.647 414 43 1 1 410 126 539 0.0 756
MsaG037030 MtrunA17_Chr7g0232841 84.337 415 45 5 1 410 129 528 0.0 675
MsaG037030 MtrunA17_Chr7g0232981 77.349 415 65 5 1 410 126 516 0.0 614
MsaG037030 MtrunA17_Chr5g0399801 44.609 473 179 10 1 410 637 1089 1.45e-117 367
MsaG037030 MtrunA17_Chr4g0003511 43.644 472 184 9 1 410 601 1052 1.03e-114 359
MsaG037030 MtrunA17_Chr4g0003501 43.008 472 185 9 1 410 506 955 4.08e-113 353
MsaG037030 MtrunA17_Chr7g0232751 90.756 119 11 0 292 410 1 119 5.25e-74 226
MsaG037030 MtrunA17_Chr7g0232871 91.818 110 9 0 201 310 1 110 1.60e-65 204
MsaG037030 MtrunA17_Chr1g0170651 32.653 490 203 13 2 410 103 546 6.20e-65 217
MsaG037030 MtrunA17_Chr7g0252211 33.401 494 228 15 2 410 280 757 1.16e-64 220
MsaG037030 MtrunA17_Chr3g0080761 31.753 485 229 15 2 405 196 659 1.60e-63 215
MsaG037030 MtrunA17_Chr6g0476111 33.469 493 228 15 2 410 218 694 1.69e-63 216
MsaG037030 MtrunA17_Chr7g0232761 79.259 135 26 1 1 135 142 274 7.28e-63 203
MsaG037030 MtrunA17_Chr6g0476121 33.469 493 228 15 2 410 167 643 2.97e-62 211
MsaG037030 MtrunA17_Chr7g0232861 81.667 120 20 1 1 120 125 242 2.46e-61 198
MsaG037030 MtrunA17_Chr8g0354821 31.393 481 231 18 3 405 224 683 1.98e-59 205
MsaG037030 MtrunA17_Chr8g0368911 31.718 454 218 15 1 379 312 748 2.95e-57 200
MsaG037030 MtrunA17_Chr7g0255181 31.224 474 225 14 2 405 258 700 1.04e-56 197
MsaG037030 MtrunA17_Chr8g0368901 31.498 454 219 14 1 379 319 755 1.71e-56 198
MsaG037030 MtrunA17_Chr8g0369301 31.718 454 218 13 1 379 299 735 2.53e-53 189
MsaG037030 MtrunA17_Chr1g0163051 29.630 459 232 13 1 386 211 651 9.44e-50 178
MsaG037030 MtrunA17_Chr6g0467421 28.540 452 230 15 1 379 131 562 2.43e-49 175
MsaG037030 MtrunA17_Chr6g0467381 28.761 452 229 15 1 379 131 562 3.14e-49 175
MsaG037030 MtrunA17_Chr6g0467531 28.761 452 229 15 1 379 131 562 9.99e-48 171
MsaG037030 MtrunA17_Chr6g0467401 28.540 452 230 15 1 379 131 562 3.25e-47 169
MsaG037030 MtrunA17_Chr3g0094881 28.200 461 218 16 1 379 192 621 1.10e-43 161
MsaG037030 MtrunA17_Chr6g0467411 28.074 431 238 16 1 379 70 480 3.74e-41 152
MsaG037030 MtrunA17_Chr1g0163041 28.355 462 238 14 1 387 128 571 1.32e-37 143
MsaG037030 MtrunA17_Chr2g0306961 28.261 414 226 16 3 379 82 461 5.48e-33 129
MsaG037030 MtrunA17_Chr4g0075321 37.000 200 86 5 215 410 901 1064 7.31e-30 122
MsaG037030 MtrunA17_Chr5g0403241 35.859 198 91 5 215 410 1344 1507 1.25e-28 119
MsaG037030 MtrunA17_Chr8g0373821 35.071 211 95 7 200 405 360 533 9.87e-27 112
MsaG037030 MtrunA17_Chr1g0190311 32.536 209 108 8 202 408 465 642 2.80e-18 87.0
MsaG037030 MtrunA17_Chr1g0183321 30.476 210 104 7 202 405 568 741 2.00e-16 81.6
MsaG037030 MtrunA17_Chr7g0262001 28.934 197 101 7 215 405 534 697 8.60e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037030 AT2G35160.5 42.947 475 163 13 1 410 327 758 5.94e-108 335
MsaG037030 AT2G35160.2 42.947 475 163 13 1 410 363 794 9.29e-108 335
MsaG037030 AT2G35160.3 42.947 475 163 13 1 410 363 794 9.29e-108 335
MsaG037030 AT2G35160.1 42.947 475 163 13 1 410 363 794 9.29e-108 335
MsaG037030 AT2G35160.4 42.947 475 163 13 1 410 363 794 9.29e-108 335
MsaG037030 AT2G22740.2 41.458 480 194 13 1 410 328 790 3.48e-105 328
MsaG037030 AT2G22740.1 41.458 480 194 13 1 410 328 790 3.48e-105 328
MsaG037030 AT5G13960.2 33.130 492 233 14 1 410 13 490 1.56e-62 209
MsaG037030 AT5G13960.1 33.130 492 233 14 1 410 147 624 2.28e-61 209
MsaG037030 AT1G73100.1 30.544 478 238 14 2 405 207 664 3.47e-58 201
MsaG037030 AT5G04940.2 30.608 477 235 14 3 405 211 665 6.13e-57 198
MsaG037030 AT5G04940.1 30.608 477 235 14 3 405 211 665 6.13e-57 198
MsaG037030 AT4G13460.1 29.646 452 227 12 1 379 203 636 2.25e-46 169
MsaG037030 AT4G13460.2 29.646 452 227 12 1 379 203 636 2.25e-46 169
MsaG037030 AT2G33290.1 29.075 454 226 12 1 379 205 637 2.75e-46 169
MsaG037030 AT2G33290.3 29.075 454 226 12 1 379 205 637 2.75e-46 169
MsaG037030 AT2G33290.4 29.075 454 226 12 1 379 205 637 2.75e-46 169
MsaG037030 AT2G33290.2 29.075 454 226 12 1 379 205 637 2.75e-46 169
MsaG037030 AT2G24740.1 29.474 475 231 16 2 405 309 750 1.58e-43 162
MsaG037030 AT1G17770.1 27.495 491 241 18 2 405 226 688 5.67e-42 157
MsaG037030 AT5G47150.1 44.231 156 69 4 2 145 175 324 1.17e-25 106
MsaG037030 AT2G23740.3 34.375 192 95 7 215 405 1216 1377 6.70e-23 102
MsaG037030 AT2G23740.6 34.375 192 95 7 215 405 1216 1377 6.70e-23 102
MsaG037030 AT2G23740.2 34.375 192 95 7 215 405 1216 1377 6.70e-23 102
MsaG037030 AT2G23740.1 34.375 192 95 7 215 405 1209 1370 6.81e-23 102
MsaG037030 AT5G47160.1 42.484 153 69 4 2 141 261 407 1.61e-22 99.0
MsaG037030 AT5G47160.3 42.484 153 69 4 2 141 261 407 1.61e-22 99.0
MsaG037030 AT5G47160.2 42.484 153 69 4 2 141 261 407 1.61e-22 99.0
MsaG037030 AT3G04380.6 31.579 209 109 7 202 408 257 433 6.47e-19 89.0
MsaG037030 AT3G04380.5 31.579 209 109 7 202 408 241 417 7.83e-19 88.6
MsaG037030 AT3G04380.2 31.579 209 109 7 202 408 257 433 8.57e-19 88.6
MsaG037030 AT3G04380.4 31.579 209 109 7 202 408 284 460 8.84e-19 88.6
MsaG037030 AT3G04380.1 31.579 209 109 7 202 408 284 460 9.43e-19 88.6
MsaG037030 AT3G04380.3 31.579 209 109 7 202 408 268 444 1.00e-18 88.2
MsaG037030 AT3G03750.2 28.718 195 111 5 215 409 183 349 1.42e-14 75.1
MsaG037030 AT2G05900.1 27.363 201 104 7 20 183 1 196 1.88e-12 68.2
MsaG037030 AT2G23740.4 36.000 125 53 6 215 338 1216 1314 4.36e-12 68.6
MsaG037030 AT2G23740.5 36.000 125 53 6 215 338 1216 1314 4.36e-12 68.6
MsaG037030 AT1G76710.3 32.065 184 74 9 224 405 90 224 6.19e-12 67.8
MsaG037030 AT1G76710.2 32.065 184 74 9 224 405 90 224 6.51e-12 67.4
MsaG037030 AT1G76710.1 32.065 184 74 9 224 405 90 224 6.51e-12 67.4
MsaG037030 AT1G76710.4 32.065 184 74 9 224 405 90 224 6.80e-12 67.4

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATAAAGTCGAATCTATT+TGG 0.122230 7:+50441334 MsaT037030.1:CDS
GATGAGTATTTATTTAATAT+TGG 0.230586 7:+50441780 MsaT037030.1:CDS
AACATTTCGAGCAAACAAAT+TGG 0.261209 7:-50442000 None:intergenic
AACTTTATAATAACTCTAAT+AGG 0.276626 7:-50441249 None:intergenic
GGAGGTAATGTCATTAATTC+TGG 0.290555 7:+50441174 MsaT037030.1:CDS
CGAGTAATGTATTGAAAATA+TGG 0.296319 7:-50441492 None:intergenic
ACAATTGATGCTGCTAAGTA+TGG 0.305938 7:+50441942 MsaT037030.1:CDS
TTATAAAGTCGAATCTATTT+GGG 0.319297 7:+50441335 MsaT037030.1:CDS
TCAGAGAATGCAATTCCTTC+TGG 0.338329 7:+50441690 MsaT037030.1:CDS
AATTCTGGAGAATGTGATAA+AGG 0.344872 7:+50441189 MsaT037030.1:CDS
GAATCTTCGAAAAGCCATTC+AGG 0.347836 7:-50441525 None:intergenic
ACAAAAGTGTTCGTTTATAC+AGG 0.350376 7:+50441147 MsaT037030.1:CDS
TCTCATTGATTACGTGATAA+AGG 0.363505 7:+50441068 MsaT037030.1:CDS
CTCGTAACATTTATCCTGAA+TGG 0.372209 7:+50441511 MsaT037030.1:CDS
AGGTGAGATTATTGAAGATC+AGG 0.383100 7:+50441734 MsaT037030.1:CDS
TTGTCACCTACTTCAATTCC+AGG 0.384331 7:-50440991 None:intergenic
TTCATATATGGTTGATAGTA+AGG 0.403159 7:+50441842 MsaT037030.1:CDS
ATGGGAAGAGGATTCATATA+TGG 0.412825 7:+50441830 MsaT037030.1:CDS
GTGGTTAATCGTATAGATTC+TGG 0.413281 7:+50441462 MsaT037030.1:CDS
GGTGGTGGTGCACGTGGTGG+AGG 0.414640 7:+50441288 MsaT037030.1:CDS
ACAAATTGGGAGAACAACTA+TGG 0.427505 7:-50441986 None:intergenic
GGTGAGGTGTATTGTTACTA+TGG 0.435582 7:+50441309 MsaT037030.1:CDS
ACATTTCGAGCAAACAAATT+GGG 0.449844 7:-50441999 None:intergenic
ATACGATTAACCACACATAT+AGG 0.451047 7:-50441453 None:intergenic
ATTCTGGAGAATGTGATAAA+GGG 0.456300 7:+50441190 MsaT037030.1:CDS
TTCACAAGGTCTATAGTTCA+TGG 0.458760 7:+50441811 MsaT037030.1:CDS
CCCTCCACTCTTAAGAGCAC+AGG 0.461039 7:-50441586 None:intergenic
TGGCAAGAACTCAACGTTGT+TGG 0.468798 7:+50441024 MsaT037030.1:CDS
GATCAAGTGATTGATTCAAA+TGG 0.480841 7:+50442125 MsaT037030.1:CDS
CAAGACTAAGGCGAAAGGAT+GGG 0.513493 7:+50441659 MsaT037030.1:CDS
ATAAAGTTTAATTCAAAGAA+CGG 0.520311 7:+50441264 MsaT037030.1:CDS
GGTCTATAGTTCATGGGAAG+AGG 0.525490 7:+50441818 MsaT037030.1:CDS
AAAATGATTGGAAATGTCCC+TGG 0.526919 7:+50440973 None:intergenic
AAGACGAAAGCATGTTATTG+TGG 0.528019 7:+50442155 MsaT037030.1:CDS
TAGGTGACAAGTTTCAGTAT+TGG 0.528561 7:+50441004 MsaT037030.1:CDS
TCAAGACTAAGGCGAAAGGA+TGG 0.530216 7:+50441658 MsaT037030.1:CDS
GCCTGTGCTCTTAAGAGTGG+AGG 0.530694 7:+50441585 MsaT037030.1:CDS
CAAAAGTGTTCGTTTATACA+GGG 0.533398 7:+50441148 MsaT037030.1:CDS
AAGACTAAGGCGAAAGGATG+GGG 0.542189 7:+50441660 MsaT037030.1:CDS
AATTCAAAGAACGGTGGTGG+TGG 0.550993 7:+50441273 MsaT037030.1:CDS
GGTGGTGGTGGTGCACGTGG+TGG 0.552190 7:+50441285 MsaT037030.1:CDS
AGAAGCTTCAAGTGGTAAAG+AGG 0.552219 7:+50441887 MsaT037030.1:CDS
ATGATCGATGTGATAGTCGT+AGG 0.556272 7:+50441916 MsaT037030.1:CDS
TTTGTTATAGATATTTCCAA+TGG 0.556582 7:+50441417 MsaT037030.1:CDS
CTAAGTATGGTAACGTGGCA+AGG 0.558339 7:+50441955 MsaT037030.1:CDS
TTTAATTCAAAGAACGGTGG+TGG 0.569178 7:+50441270 MsaT037030.1:CDS
AACGGTGGTGGTGGTGCACG+TGG 0.573546 7:+50441282 MsaT037030.1:CDS
TTTGCCTGTGCTCTTAAGAG+TGG 0.575325 7:+50441582 MsaT037030.1:CDS
TGGTGCACGTGGTGGAGGTG+AGG 0.581579 7:+50441293 MsaT037030.1:CDS
CCTGTGCTCTTAAGAGTGGA+GGG 0.588175 7:+50441586 MsaT037030.1:CDS
TCACAAGGTCTATAGTTCAT+GGG 0.590256 7:+50441812 MsaT037030.1:CDS
TATTGGAAATTATATTCACA+AGG 0.594119 7:+50441797 MsaT037030.1:CDS
AAAGTCAGCTCATTCATGCT+CGG 0.596521 7:-50442083 None:intergenic
TTTACAATGATGATGATCTG+AGG 0.599652 7:+50442024 MsaT037030.1:CDS
GTGTTCGTTTATACAGGGGA+AGG 0.599990 7:+50441153 MsaT037030.1:CDS
AATCTCATCAATACAGTCGA+CGG 0.610597 7:-50442193 None:intergenic
TTCGTTTATACAGGGGAAGG+AGG 0.611584 7:+50441156 MsaT037030.1:CDS
AATGTCCCTGGAATTGAAGT+AGG 0.621360 7:+50440985 MsaT037030.1:CDS
TAATCGTATAGATTCTGGCA+AGG 0.632162 7:+50441467 MsaT037030.1:CDS
TGTCACCTACTTCAATTCCA+GGG 0.640833 7:-50440990 None:intergenic
TCACAAATAAAACTTCCAGA+AGG 0.644047 7:-50441705 None:intergenic
CAACGCTTAGAAGCTTCAAG+TGG 0.647600 7:+50441879 MsaT037030.1:CDS
AGAACATTTACGCCCAACAG+AGG 0.655904 7:-50441547 None:intergenic
AAAAGTGTTCGTTTATACAG+GGG 0.661822 7:+50441149 MsaT037030.1:CDS
AGAAGCTGTTCCTATATGTG+TGG 0.665038 7:+50441443 MsaT037030.1:CDS
ACTATCACATCGATCATCAA+CGG 0.674602 7:-50441910 None:intergenic
AAGTTTAATTCAAAGAACGG+TGG 0.694907 7:+50441267 MsaT037030.1:CDS
GCTGCATATAGCATGATGTG+TGG 0.717627 7:-50442050 None:intergenic
TGCTGCTAAGTATGGTAACG+TGG 0.728265 7:+50441950 MsaT037030.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50440976 50442208 50440976 ID=MsaG037030
Chr7 mRNA 50440976 50442208 50440976 ID=MsaT037030.1;Parent=MsaG037030
Chr7 exon 50440976 50442208 50440976 ID=MsaT037030.1.exon1;Parent=MsaT037030.1
Chr7 CDS 50440976 50442208 50440976 ID=cds.MsaT037030.1;Parent=MsaT037030.1
Gene Sequence

>MsaT037030.1

ATGATTGGAAATGTCCCTGGAATTGAAGTAGGTGACAAGTTTCAGTATTGGCAAGAACTCAACGTTGTTGGTCTTCATCGTCAGAATCTAAGTCTCATTGATTACGTGATAAAGGATGATAATTTAATTGCAACTAGCATTGTTTCTTGTCATTTTGATGATATGGATGATACAAAAGTGTTCGTTTATACAGGGGAAGGAGGTAATGTCATTAATTCTGGAGAATGTGATAAAGGGAATCTTGCTCTGATGAATAGTTTTGCTGTTAAGAATCCTATTAGAGTTATTATAAAGTTTAATTCAAAGAACGGTGGTGGTGGTGCACGTGGTGGAGGTGAGGTGTATTGTTACTATGGTCTTTATAAAGTCGAATCTATTTGGGAAAAAAAAGGGAAAGTAGATTTTAAATTTTGTTTGGTTAGATTGCCTGATCAGAAATTTTTTGTTATAGATATTTCCAATGGAAAAGAAGCTGTTCCTATATGTGTGGTTAATCGTATAGATTCTGGCAAGGTTCCATATTTTCAATACATTACTCGTAACATTTATCCTGAATGGCTTTTCGAAGATTCCTCTGTTGGGCGTAAATGTTCTGATTCAATGAAATTTGCCTGTGCTCTTAAGAGTGGAGGGAAAATTCCTAGAGTAAAGAAAAATGAGATTAAATTTAAGCTTCAAATTTTCAAGACTAAGGCGAAAGGATGGGGTGTGAGATCAGAGAATGCAATTCCTTCTGGAAGTTTTATTTGTGAGTATTTAGGTGAGATTATTGAAGATCAGGAAGCTTTGAAAATAGTTGATAATGATGAGTATTTATTTAATATTGGAAATTATATTCACAAGGTCTATAGTTCATGGGAAGAGGATTCATATATGGTTGATAGTAAGGAGATGAGTTCATATCAACGCTTAGAAGCTTCAAGTGGTAAAGAGGCCGTTGATGATCGATGTGATAGTCGTAGGTTTACAATTGATGCTGCTAAGTATGGTAACGTGGCAAGGTTTATCAACCATAGTTGTTCTCCCAATTTGTTTGCTCGAAATGTTCTTTACAATGATGATGATCTGAGGATTCCACACATCATGCTATATGCAGCTGAGAACATTCCGAGCATGAATGAGCTGACTTTGAATTACAATTACAAGCTCGATCAAGTGATTGATTCAAATGGAAATTTAAAGACGAAAGCATGTTATTGTGGAGCTTCTGAATGCATCCGTCGACTGTATTGA
Protein sequence

>MsaT037030.1

MIGNVPGIEVGDKFQYWQELNVVGLHRQNLSLIDYVIKDDNLIATSIVSCHFDDMDDTKVFVYTGEGGNVINSGECDKGNLALMNSFAVKNPIRVIIKFNSKNGGGGARGGGEVYCYYGLYKVESIWEKKGKVDFKFCLVRLPDQKFFVIDISNGKEAVPICVVNRIDSGKVPYFQYITRNIYPEWLFEDSSVGRKCSDSMKFACALKSGGKIPRVKKNEIKFKLQIFKTKAKGWGVRSENAIPSGSFICEYLGEIIEDQEALKIVDNDEYLFNIGNYIHKVYSSWEEDSYMVDSKEMSSYQRLEASSGKEAVDDRCDSRRFTIDAAKYGNVARFINHSCSPNLFARNVLYNDDDLRIPHIMLYAAENIPSMNELTLNYNYKLDQVIDSNGNLKTKACYCGASECIRRLY*