AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037041

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037041 MtrunA17_Chr7g0232681 66.327 196 18 2 1 148 1 196 8.43e-87 252
MsaG037041 MtrunA17_Chr7g0232701 63.043 138 13 2 48 148 1 137 1.01e-52 163
MsaG037041 MtrunA17_Chr7g0232671 70.130 77 23 0 73 149 176 252 1.61e-33 118
MsaG037041 MtrunA17_Chr3g0145121 55.224 67 28 1 83 147 214 280 3.40e-19 81.6
MsaG037041 MtrunA17_Chr4g0035891 53.846 65 30 0 81 145 208 272 4.24e-19 81.6
MsaG037041 MtrunA17_Chr3g0140331 59.016 61 25 0 84 144 253 313 4.67e-19 82.0
MsaG037041 MtrunA17_Chr2g0326771 52.857 70 33 0 83 152 215 284 1.07e-18 80.5
MsaG037041 MtrunA17_Chr6g0461591 45.455 88 46 1 65 152 175 260 1.25e-18 79.7
MsaG037041 MtrunA17_Chr4g0028961 56.452 62 27 0 84 145 232 293 1.30e-18 80.5
MsaG037041 MtrunA17_Chr1g0199671 53.731 67 31 0 84 150 242 308 3.08e-18 79.7
MsaG037041 MtrunA17_Chr1g0146231 56.452 62 27 0 84 145 234 295 4.76e-18 79.0
MsaG037041 MtrunA17_Chr7g0253101 56.452 62 27 0 84 145 188 249 7.22e-18 77.8
MsaG037041 MtrunA17_Chr8g0340401 54.839 62 28 0 84 145 226 287 1.75e-17 77.4
MsaG037041 MtrunA17_Chr5g0404981 42.391 92 51 1 63 152 279 370 2.10e-17 77.8
MsaG037041 MtrunA17_Chr8g0372291 49.296 71 34 1 84 152 218 288 3.91e-17 76.3
MsaG037041 MtrunA17_Chr1g0213161 54.839 62 28 0 84 145 152 213 8.09e-17 74.3
MsaG037041 MtrunA17_Chr1g0169431 53.731 67 29 1 84 148 172 238 3.05e-16 73.2
MsaG037041 MtrunA17_Chr5g0395391 48.611 72 35 1 84 153 270 341 4.31e-15 70.9
MsaG037041 MtrunA17_Chr5g0395391 50.000 62 31 0 84 145 168 229 1.12e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037041 AT3G60530.1 56.452 62 27 0 84 145 158 219 3.64e-20 83.6
MsaG037041 AT5G25830.1 57.143 63 27 0 83 145 218 280 9.06e-20 84.0
MsaG037041 AT2G45050.1 54.839 62 28 0 84 145 179 240 1.09e-19 82.8
MsaG037041 AT4G32890.1 56.452 62 27 0 84 145 197 258 4.45e-19 82.0
MsaG037041 AT4G36240.1 29.592 196 86 6 2 145 30 225 7.69e-19 80.1
MsaG037041 AT4G34680.1 32.317 164 68 3 20 145 83 241 1.42e-18 80.1
MsaG037041 AT4G34680.2 32.317 164 68 3 20 145 83 241 1.42e-18 80.1
MsaG037041 AT1G08000.2 56.716 67 27 1 84 148 218 284 1.81e-18 80.1
MsaG037041 AT1G08000.1 56.716 67 27 1 84 148 218 284 1.81e-18 80.1
MsaG037041 AT3G54810.1 50.633 79 38 1 73 150 217 295 1.82e-18 80.5
MsaG037041 AT3G54810.2 50.633 79 38 1 73 150 217 295 1.82e-18 80.5
MsaG037041 AT2G28340.1 58.333 60 25 0 86 145 193 252 2.58e-18 79.7
MsaG037041 AT5G66320.2 53.968 63 29 0 83 145 248 310 3.09e-18 80.1
MsaG037041 AT5G66320.1 53.968 63 29 0 83 145 248 310 3.09e-18 80.1
MsaG037041 AT3G45170.1 51.471 68 31 1 86 151 117 184 1.01e-17 76.6
MsaG037041 AT1G08010.2 53.226 62 29 0 84 145 220 281 3.81e-17 76.6
MsaG037041 AT1G08010.1 53.226 62 29 0 84 145 220 281 3.81e-17 76.6
MsaG037041 AT1G08010.3 53.226 62 29 0 84 145 220 281 3.81e-17 76.6
MsaG037041 AT3G51080.1 51.429 70 32 1 84 151 221 290 4.58e-17 76.6
MsaG037041 AT3G24050.1 53.731 67 29 1 84 148 194 260 1.19e-16 75.1

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGGAGGTTGGGTCCTT+TGG 0.187764 7:-50597902 MsaT037041.1:CDS
GTTCTGGCAATCCTCATCTT+TGG 0.213267 7:-50598621 MsaT037041.1:CDS
TTTAGAAAAGGTCATCAAAT+TGG 0.233940 7:-50598037 MsaT037041.1:CDS
CATCAAATTGGATGAATTAA+TGG 0.255209 7:-50598025 MsaT037041.1:CDS
ACAGGAGGCACTAGAAAATT+TGG 0.257280 7:-50598094 MsaT037041.1:CDS
CAATGGAGGTTGGGTCCTTT+GGG 0.260138 7:-50597901 MsaT037041.1:CDS
AAGCTAAATGGATTGGTTAA+AGG 0.267058 7:-50597841 MsaT037041.1:CDS
CAAATTCTCTGATGATGATT+AGG 0.271705 7:+50597952 None:intergenic
TTCTGGCAATCCTCATCTTT+GGG 0.283554 7:-50598620 MsaT037041.1:intron
CCTCCATTGAGTAGTTTCTT+TGG 0.310305 7:+50597915 None:intergenic
GGATTACGATACAAGCTAAA+TGG 0.317668 7:-50597853 MsaT037041.1:CDS
AGCTAAATGGATTGGTTAAA+GGG 0.347180 7:-50597840 MsaT037041.1:CDS
AAATTCTCTGATGATGATTA+GGG 0.356028 7:+50597953 None:intergenic
AACATGAAGTGGGTCAGTTC+TGG 0.366216 7:-50598637 MsaT037041.1:CDS
ATCAAATTGGATGAATTAAT+GGG 0.418542 7:-50598024 MsaT037041.1:CDS
TCAAAAGCTGGACTTGCCTT+AGG 0.449589 7:+50597811 None:intergenic
CTTGTATCAATATTGAAAGA+TGG 0.486236 7:+50597979 None:intergenic
GAAACTACTCAATGGAGGTT+GGG 0.488791 7:-50597910 MsaT037041.1:CDS
TCATTCAAATTTACACAAGA+AGG 0.495479 7:-50597779 MsaT037041.1:CDS
ACGATACAAGCTAAATGGAT+TGG 0.515501 7:-50597848 MsaT037041.1:CDS
ACACAAGAAGGCGATGAGAA+AGG 0.520810 7:-50597767 MsaT037041.1:CDS
AGAAACTACTCAATGGAGGT+TGG 0.521850 7:-50597911 MsaT037041.1:CDS
TGACATGGAGAATTTAGAAA+AGG 0.524637 7:-50598049 MsaT037041.1:CDS
AATGGAGGTTGGGTCCTTTG+GGG 0.535179 7:-50597900 MsaT037041.1:CDS
TGATTTCTGATATCAAAAGC+TGG 0.547788 7:+50597799 None:intergenic
GGTTAAAGGGTATAGACCTA+AGG 0.562082 7:-50597827 MsaT037041.1:CDS
AGATTTGAGTAAACATGAAG+TGG 0.567173 7:-50598648 MsaT037041.1:CDS
AAGAAGGCGATGAGAAAGGA+AGG 0.572140 7:-50597763 MsaT037041.1:CDS
AGACCAAAGAAACTACTCAA+TGG 0.572896 7:-50597918 MsaT037041.1:CDS
CACAAAGTATTCTTCCCCAA+AGG 0.604152 7:+50597886 None:intergenic
GAATCTTGATTATATCCCAC+AGG 0.607559 7:-50598112 MsaT037041.1:CDS
AATACTTTGTGCAATGCATG+TGG 0.615784 7:-50597874 MsaT037041.1:CDS
AGGCACTAGAAAATTTGGAA+TGG 0.632247 7:-50598089 MsaT037041.1:CDS
ATAATACTAACCCAAAGATG+AGG 0.642509 7:+50598610 None:intergenic
CCAAAGAAACTACTCAATGG+AGG 0.662722 7:-50597915 MsaT037041.1:CDS
GATTTGAGTAAACATGAAGT+GGG 0.670715 7:-50598647 MsaT037041.1:CDS
TCTTGATTATATCCCACAGG+AGG 0.714474 7:-50598109 MsaT037041.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50597747 50598693 50597747 ID=MsaG037041
Chr7 mRNA 50597747 50598693 50597747 ID=MsaT037041.1;Parent=MsaG037041
Chr7 exon 50597747 50598135 50597747 ID=MsaT037041.1.exon2;Parent=MsaT037041.1
Chr7 CDS 50597747 50598135 50597747 ID=cds.MsaT037041.1;Parent=MsaT037041.1
Chr7 exon 50598621 50598693 50598621 ID=MsaT037041.1.exon1;Parent=MsaT037041.1
Chr7 CDS 50598621 50598693 50598621 ID=cds.MsaT037041.1;Parent=MsaT037041.1
Gene Sequence

>MsaT037041.1

ATGGCTAATCAGAGAGAAGAACAAGATTTGAGTAAACATGAAGTGGGTCAGTTCTGGCAATCCTCATCTTTGGGGAATCTTGATTATATCCCACAGGAGGCACTAGAAAATTTGGAATGGTACACTACTTTTTGGGATGACATGGAGAATTTAGAAAAGGTCATCAAATTGGATGAATTAATGGGTAAGAGAAAAACACAAGAAAATCCATCTTTCAATATTGATACAAGTGTCCCTAATCATCATCAGAGAATTTGCAGCGATTGTGAGACCAAAGAAACTACTCAATGGAGGTTGGGTCCTTTGGGGAAGAATACTTTGTGCAATGCATGTGGATTACGATACAAGCTAAATGGATTGGTTAAAGGGTATAGACCTAAGGCAAGTCCAGCTTTTGATATCAGAAATCATTCAAATTTACACAAGAAGGCGATGAGAAAGGAAGGAAAAACAATCATTTAG
Protein sequence

>MsaT037041.1

MANQREEQDLSKHEVGQFWQSSSLGNLDYIPQEALENLEWYTTFWDDMENLEKVIKLDELMGKRKTQENPSFNIDTSVPNHHQRICSDCETKETTQWRLGPLGKNTLCNACGLRYKLNGLVKGYRPKASPAFDIRNHSNLHKKAMRKEGKTII*