AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037044

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037044 MtrunA17_Chr7g0232811 90.406 542 48 2 1 541 1 539 0.0 1006
MsaG037044 MtrunA17_Chr7g0232841 87.661 543 50 5 1 541 1 528 0.0 930
MsaG037044 MtrunA17_Chr7g0232981 80.996 542 76 5 1 541 1 516 0.0 853
MsaG037044 MtrunA17_Chr7g0232861 85.477 241 34 1 7 247 3 242 1.85e-141 408
MsaG037044 MtrunA17_Chr5g0399801 41.148 610 266 12 2 541 503 1089 2.57e-132 411
MsaG037044 MtrunA17_Chr7g0232761 74.194 279 50 4 1 262 1 274 2.85e-130 380
MsaG037044 MtrunA17_Chr4g0003511 42.486 539 224 10 70 541 533 1052 1.02e-125 393
MsaG037044 MtrunA17_Chr4g0003501 42.329 541 220 11 70 541 438 955 1.43e-122 383
MsaG037044 MtrunA17_Chr7g0232751 94.958 119 6 0 423 541 1 119 2.82e-77 239
MsaG037044 MtrunA17_Chr1g0170651 32.286 573 248 16 61 541 22 546 4.81e-73 243
MsaG037044 MtrunA17_Chr7g0252211 31.765 510 252 13 112 541 264 757 2.83e-67 231
MsaG037044 MtrunA17_Chr7g0232871 92.727 110 8 0 332 441 1 110 1.25e-66 211
MsaG037044 MtrunA17_Chr3g0080761 31.868 546 262 19 78 536 137 659 3.31e-66 226
MsaG037044 MtrunA17_Chr6g0476111 31.961 510 250 15 112 541 202 694 3.64e-64 222
MsaG037044 MtrunA17_Chr8g0354821 31.502 546 262 21 78 536 163 683 1.06e-63 220
MsaG037044 MtrunA17_Chr6g0476121 32.353 510 248 16 112 541 151 643 3.00e-63 218
MsaG037044 MtrunA17_Chr8g0368911 33.333 474 225 16 110 510 293 748 5.99e-63 220
MsaG037044 MtrunA17_Chr8g0368901 32.285 477 226 16 110 510 300 755 1.20e-59 211
MsaG037044 MtrunA17_Chr7g0255181 30.597 536 259 19 79 536 200 700 7.21e-57 202
MsaG037044 MtrunA17_Chr8g0369301 31.356 472 237 12 110 510 280 735 9.44e-57 202
MsaG037044 MtrunA17_Chr1g0163051 29.769 477 249 12 110 517 192 651 6.93e-54 193
MsaG037044 MtrunA17_Chr6g0467421 29.614 466 242 14 113 510 115 562 1.59e-52 187
MsaG037044 MtrunA17_Chr6g0467381 30.043 466 240 15 113 510 115 562 5.52e-52 186
MsaG037044 MtrunA17_Chr6g0467531 29.614 466 242 14 113 510 115 562 7.48e-52 186
MsaG037044 MtrunA17_Chr6g0467401 29.828 466 241 15 113 510 115 562 3.93e-50 181
MsaG037044 MtrunA17_Chr3g0094881 28.083 527 248 18 72 510 138 621 1.27e-45 169
MsaG037044 MtrunA17_Chr6g0467411 28.953 449 246 17 113 510 54 480 2.26e-44 163
MsaG037044 MtrunA17_Chr1g0163041 28.216 482 254 14 110 518 109 571 3.19e-41 156
MsaG037044 MtrunA17_Chr2g0306961 27.252 433 245 16 113 510 64 461 1.14e-35 139
MsaG037044 MtrunA17_Chr4g0075321 35.500 200 89 5 346 541 901 1064 7.03e-28 119
MsaG037044 MtrunA17_Chr5g0403241 34.343 198 94 5 346 541 1344 1507 5.80e-27 116
MsaG037044 MtrunA17_Chr8g0373821 34.197 193 88 5 346 536 378 533 1.06e-24 107
MsaG037044 MtrunA17_Chr1g0183321 31.429 210 102 7 333 536 568 741 1.31e-18 89.7
MsaG037044 MtrunA17_Chr1g0190311 32.057 209 109 8 333 539 465 642 2.11e-18 89.0
MsaG037044 MtrunA17_Chr7g0262001 30.952 210 103 9 333 536 524 697 5.39e-14 75.1
MsaG037044 MtrunA17_Chr6g0460921 25.225 222 124 9 320 540 239 419 1.41e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037044 AT2G35160.5 41.053 570 218 15 44 541 235 758 2.53e-117 364
MsaG037044 AT2G35160.2 41.053 570 218 15 44 541 271 794 5.19e-117 364
MsaG037044 AT2G35160.3 41.053 570 218 15 44 541 271 794 5.19e-117 364
MsaG037044 AT2G35160.1 41.053 570 218 15 44 541 271 794 5.19e-117 364
MsaG037044 AT2G35160.4 41.053 570 218 15 44 541 271 794 5.19e-117 364
MsaG037044 AT2G22740.2 41.155 537 223 14 82 541 270 790 4.06e-112 351
MsaG037044 AT2G22740.1 41.155 537 223 14 82 541 270 790 4.06e-112 351
MsaG037044 AT5G13960.2 33.266 493 238 14 127 541 11 490 1.72e-64 218
MsaG037044 AT5G13960.1 33.266 493 238 14 127 541 145 624 2.84e-64 221
MsaG037044 AT1G73100.1 29.740 538 286 17 70 536 148 664 8.04e-58 204
MsaG037044 AT5G04940.2 30.785 484 236 17 127 536 207 665 4.11e-56 199
MsaG037044 AT5G04940.1 30.785 484 236 17 127 536 207 665 4.11e-56 199
MsaG037044 AT2G33290.1 28.920 574 307 19 22 524 104 647 4.95e-51 185
MsaG037044 AT2G33290.3 28.920 574 307 19 22 524 104 647 4.95e-51 185
MsaG037044 AT2G33290.4 28.920 574 307 19 22 524 104 647 4.95e-51 185
MsaG037044 AT2G33290.2 28.920 574 307 19 22 524 104 647 4.95e-51 185
MsaG037044 AT4G13460.1 29.485 485 252 13 97 510 171 636 6.78e-50 182
MsaG037044 AT4G13460.2 29.485 485 252 13 97 510 171 636 6.78e-50 182
MsaG037044 AT2G24740.1 30.316 475 230 16 130 536 309 750 1.25e-47 177
MsaG037044 AT1G17770.1 28.016 489 250 16 127 536 223 688 8.40e-45 168
MsaG037044 AT5G47150.1 37.339 233 113 8 67 272 98 324 2.24e-27 113
MsaG037044 AT5G47160.1 33.219 292 154 11 5 268 129 407 2.28e-24 105
MsaG037044 AT5G47160.3 33.219 292 154 11 5 268 129 407 2.28e-24 105
MsaG037044 AT5G47160.2 33.219 292 154 11 5 268 129 407 2.28e-24 105
MsaG037044 AT3G04380.2 33.014 209 106 8 333 539 257 433 1.56e-19 92.0
MsaG037044 AT3G04380.6 33.014 209 106 8 333 539 257 433 1.57e-19 92.0
MsaG037044 AT3G04380.4 33.014 209 106 8 333 539 284 460 1.72e-19 92.0
MsaG037044 AT3G04380.1 33.014 209 106 8 333 539 284 460 1.73e-19 92.0
MsaG037044 AT3G04380.3 33.014 209 106 8 333 539 268 444 1.75e-19 92.0
MsaG037044 AT3G04380.5 33.014 209 106 8 333 539 241 417 1.78e-19 91.7
MsaG037044 AT3G03750.2 28.718 195 111 5 346 540 183 349 7.31e-15 76.6
MsaG037044 AT1G04050.2 28.641 206 110 7 333 536 548 718 1.59e-14 77.0
MsaG037044 AT1G04050.1 28.641 206 110 7 333 536 548 718 1.59e-14 77.0
MsaG037044 AT1G04050.3 28.641 206 110 7 333 536 444 614 1.73e-14 77.0
MsaG037044 AT2G05900.1 27.586 232 116 10 148 342 1 217 9.77e-13 69.7
MsaG037044 AT1G76710.3 30.222 225 101 12 317 536 51 224 1.54e-12 70.5
MsaG037044 AT1G76710.4 30.222 225 101 12 317 536 51 224 1.69e-12 70.1
MsaG037044 AT1G76710.2 30.088 226 100 12 317 536 51 224 1.73e-12 70.1
MsaG037044 AT1G76710.1 30.088 226 100 12 317 536 51 224 1.73e-12 70.1

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATAAAGTTGAATCTATT+TGG 0.088477 7:-50631371 MsaT037044.1:CDS
TTTACCACTTGAAGCTTCTA+AGG 0.221245 7:+50630810 None:intergenic
GATGAGTATTTATTTAATAT+TGG 0.230586 7:-50630913 MsaT037044.1:CDS
AACATTTCGAGCAAACAAAT+TGG 0.261209 7:+50630693 None:intergenic
AACTTTATAATAACTCTAAT+AGG 0.276626 7:+50631453 None:intergenic
GGAGGTAATGTCATTAATTC+TGG 0.290555 7:-50631528 MsaT037044.1:CDS
TTATAAAGTTGAATCTATTT+GGG 0.319297 7:-50631370 MsaT037044.1:CDS
ACGATTGATGCTGCTAAGTA+TGG 0.327893 7:-50630751 MsaT037044.1:CDS
TCAGAGAATGCAATTCCTTC+TGG 0.338329 7:-50631003 MsaT037044.1:CDS
GAATCATTGAAAAGCCATTC+AGG 0.342986 7:+50631180 None:intergenic
AATTCTGGAGAATGTGATAA+AGG 0.344872 7:-50631513 MsaT037044.1:CDS
ACAAAAGTGTTCGTTTATAC+AGG 0.350376 7:-50631555 MsaT037044.1:CDS
TCTCATTGATTACGTGATAA+AGG 0.363505 7:-50631634 MsaT037044.1:CDS
TTTAAATGCAGTTTCTTGAT+TGG 0.364503 7:+50631951 None:intergenic
CTCGTAACATTTATCCTGAA+TGG 0.372209 7:-50631194 MsaT037044.1:CDS
TTTCTTGATTGGTGTTTCCT+TGG 0.377579 7:+50631962 None:intergenic
TTGTCACCTACTTCAATTCC+AGG 0.384331 7:+50631711 None:intergenic
CTTGAAATGGTTTGTGGTGA+TGG 0.386469 7:+50632075 None:intergenic
TTCATATATGGTTGATAGTA+AGG 0.403159 7:-50630851 MsaT037044.1:CDS
GTGGTTAATCGTATAGATTC+TGG 0.413281 7:-50631243 MsaT037044.1:CDS
GTGGGAAGAGGATTCATATA+TGG 0.420840 7:-50630863 MsaT037044.1:CDS
ACAAATTGGGAGAACAACTA+TGG 0.427505 7:+50630707 None:intergenic
GGTGAGGTGTATTGTTACTA+TGG 0.435582 7:-50631396 MsaT037044.1:CDS
ACATTTCGAGCAAACAAATT+GGG 0.449844 7:+50630694 None:intergenic
ATACGATTAACCACACATAT+AGG 0.451047 7:+50631252 None:intergenic
TTCCTGTTGGAGACTTGAAA+TGG 0.453907 7:+50632062 None:intergenic
TGGCAAGAACTCAATGTTGT+TGG 0.454774 7:-50631678 MsaT037044.1:CDS
ATTCTGGAGAATGTGATAAA+GGG 0.456300 7:-50631512 MsaT037044.1:CDS
GGTGGTGGTGCACGTGGTGG+AGG 0.461405 7:-50631417 MsaT037044.1:CDS
AACCATTTCAAGTCTCCAAC+AGG 0.470536 7:-50632064 MsaT037044.1:CDS
CCCTCCACTCTTAAGAGCAC+AGG 0.479558 7:+50631107 None:intergenic
GATCAAGTGATTGATTCAAA+TGG 0.480841 7:-50630568 MsaT037044.1:CDS
AAAATTATTGGAAATGTCCC+TGG 0.496911 7:-50631729 MsaT037044.1:CDS
TGGAGACTTGAAATGGTTTG+TGG 0.503466 7:+50632069 None:intergenic
GGTCTATAGTTCGTGGGAAG+AGG 0.504364 7:-50630875 MsaT037044.1:CDS
CAAGACTAAGGCGAAAGGAT+GGG 0.513493 7:-50631034 MsaT037044.1:CDS
AGGTGAGATTATTGAAGATG+AGG 0.514537 7:-50630959 MsaT037044.1:CDS
TTTCTTTCTTCTCGAGTCAT+CGG 0.519338 7:+50631881 None:intergenic
CGTGCTCTCTGTGGAAAACT+CGG 0.520054 7:-50631834 MsaT037044.1:CDS
ATAAAGTTTAATTCAAAGAA+CGG 0.520311 7:-50631438 MsaT037044.1:CDS
TTTGTTAAAGATATTTCCAA+TGG 0.523563 7:-50631288 MsaT037044.1:CDS
AAGACGAAAGCATGTTATTG+TGG 0.528019 7:-50630538 MsaT037044.1:CDS
TAGGTGACAAGTTTCAGTAT+TGG 0.528561 7:-50631698 MsaT037044.1:CDS
TCAAGACTAAGGCGAAAGGA+TGG 0.530216 7:-50631035 MsaT037044.1:CDS
CAAAAGTGTTCGTTTATACA+GGG 0.533398 7:-50631554 MsaT037044.1:CDS
AGAAGCTTCAAGTGGTAAAG+AGG 0.540440 7:-50630806 MsaT037044.1:CDS
AAGACTAAGGCGAAAGGATG+GGG 0.542189 7:-50631033 MsaT037044.1:CDS
TTACCACTTGAAGCTTCTAA+GGG 0.542917 7:+50630811 None:intergenic
CTAAGTATGGTAACGTGGCA+AGG 0.558339 7:-50630738 MsaT037044.1:CDS
ATTGATGATCGATGTGATAG+TGG 0.558469 7:-50630781 MsaT037044.1:CDS
ACTATCACATCGATCATCAA+TGG 0.565161 7:+50630783 None:intergenic
TCAAAGAAACACTGAAACCA+AGG 0.567849 7:-50631979 MsaT037044.1:CDS
TCCTGTGCTCTTAAGAGTGG+AGG 0.568183 7:-50631108 MsaT037044.1:CDS
TTTAATTCAAAGAACGGTGG+TGG 0.569178 7:-50631432 MsaT037044.1:CDS
TTCACAAGGTCTATAGTTCG+TGG 0.573569 7:-50630882 MsaT037044.1:CDS
TGGTGCACGTGGTGGAGGTG+AGG 0.581579 7:-50631412 MsaT037044.1:CDS
AGAATCCGTGACACTTCGAT+AGG 0.588664 7:+50631786 None:intergenic
TCTCTGTGGAAAACTCGGTA+AGG 0.590987 7:-50631829 MsaT037044.1:CDS
AACGGTGGTGGTGCACGTGG+TGG 0.592302 7:-50631420 MsaT037044.1:CDS
TATTGGAAATTATATTCACA+AGG 0.594119 7:-50630896 MsaT037044.1:CDS
AAAGTCAGCTCATTCATGCT+CGG 0.596521 7:+50630610 None:intergenic
TCACAAATAAAATGTCCAGA+AGG 0.599161 7:+50630988 None:intergenic
TGTTCCTGTGCTCTTAAGAG+TGG 0.599372 7:-50631111 MsaT037044.1:CDS
GTGTTCGTTTATACAGGGGA+AGG 0.599990 7:-50631549 MsaT037044.1:CDS
ATGGTTCGTAGTGGAAGATG+AGG 0.601530 7:+50632094 None:intergenic
TTCGATAGGAAGCGCGTCGG+CGG 0.603200 7:+50631800 None:intergenic
TTTACGATGATGATGATCTG+AGG 0.605700 7:-50630669 MsaT037044.1:CDS
CCTGTGCTCTTAAGAGTGGA+GGG 0.608017 7:-50631107 MsaT037044.1:CDS
AATCTCATCAATACAGTCGA+CGG 0.610597 7:+50630500 None:intergenic
TTCGTTTATACAGGGGAAGG+AGG 0.611584 7:-50631546 MsaT037044.1:CDS
ATGATCGATGTGATAGTGGT+AGG 0.614728 7:-50630777 MsaT037044.1:CDS
CGCTTCCTATCGAAGTGTCA+CGG 0.617253 7:-50631791 MsaT037044.1:CDS
GAGGAAAATTCACTCACGAT+GGG 0.618861 7:-50631755 MsaT037044.1:CDS
TCACAAGGTCTATAGTTCGT+GGG 0.620772 7:-50630881 MsaT037044.1:CDS
AATGTCCCTGGAATTGAAGT+AGG 0.621360 7:-50631717 MsaT037044.1:CDS
CACAAGGAGAGTATTGTCAA+AGG 0.629254 7:-50632003 MsaT037044.1:CDS
GGTAACTTCCGTGCTCTCTG+TGG 0.631604 7:-50631843 MsaT037044.1:CDS
TAATCGTATAGATTCTGGCA+AGG 0.632162 7:-50631238 MsaT037044.1:CDS
AGAGGAAAATTCACTCACGA+TGG 0.634330 7:-50631756 MsaT037044.1:CDS
TGTCACCTACTTCAATTCCA+GGG 0.640833 7:+50631712 None:intergenic
CAACCCTTAGAAGCTTCAAG+TGG 0.647600 7:-50630814 MsaT037044.1:CDS
CACTTCGATAGGAAGCGCGT+CGG 0.648323 7:+50631797 None:intergenic
TCTATCTGTAGAGTTCCTGT+TGG 0.652385 7:+50632049 None:intergenic
AAAAGTGTTCGTTTATACAG+GGG 0.661822 7:-50631553 MsaT037044.1:CDS
AGAAGCTGTTCCTATATGTG+TGG 0.665038 7:-50631262 MsaT037044.1:CDS
AACACATTTACGCCCAACAG+AGG 0.665095 7:+50631158 None:intergenic
TGTTCAAGAATGTCAGCACA+AGG 0.670860 7:-50632019 MsaT037044.1:CDS
TGGTTCGTAGTGGAAGATGA+GGG 0.671072 7:+50632095 None:intergenic
TTTGTGGTGATGGTTCGTAG+TGG 0.675157 7:+50632085 None:intergenic
AAGAACGGTGGTGGTGCACG+TGG 0.677631 7:-50631423 MsaT037044.1:CDS
AAGTTTAATTCAAAGAACGG+TGG 0.694907 7:-50631435 MsaT037044.1:CDS
GCTGCATATAGCATGATGTG+TGG 0.717627 7:+50630643 None:intergenic
TGCTGCTAAGTATGGTAACG+TGG 0.728265 7:-50630743 MsaT037044.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50630507 50632132 50630507 ID=MsaG037044
Chr7 mRNA 50630507 50632132 50630507 ID=MsaT037044.1;Parent=MsaG037044
Chr7 exon 50630507 50632132 50630507 ID=MsaT037044.1.exon1;Parent=MsaT037044.1
Chr7 CDS 50630507 50632132 50630507 ID=cds.MsaT037044.1;Parent=MsaT037044.1
Gene Sequence

>MsaT037044.1

ATGACTAAGACGAAGCCCTCATCTTCCACTACGAACCATCACCACAAACCATTTCAAGTCTCCAACAGGAACTCTACAGATAGAGCATTGCTGTTCAAGAATGTCAGCACAAGGAGAGTATTGTCAAAGGTTCAAAGAAACACTGAAACCAAGGAAACACCAATCAAGAAACTGCATTTAAAACGTCGTTGTGAAGATGCAACTTCAAGTAACAAGTCGTCTTTGACTTCCGATGACTCGAGAAGAAAGAAAGTAAAAACAACATTAGGTAACTTCCGTGCTCTCTGTGGAAAACTCGGTAAGGAACTTACCGCCGACGCGCTTCCTATCGAAGTGTCACGGATTCTAAAAAACAGAGGAAAATTCACTCACGATGGGAAAAAAATTATTGGAAATGTCCCTGGAATTGAAGTAGGTGACAAGTTTCAGTATTGGCAAGAACTCAATGTTGTTGGTCTTCATCGTCAGAATCTAAGTCTCATTGATTACGTGATAAAGGATGATAATTTAATTGCAACAAGCATTGTTTCTTGTCATTTTGATGATATGGATGATACAAAAGTGTTCGTTTATACAGGGGAAGGAGGTAATGTCATTAATTCTGGAGAATGTGATAAAGGGAATCTTGCTCTGATGAATAGTTTTGCTGTTAAGAATCCTATTAGAGTTATTATAAAGTTTAATTCAAAGAACGGTGGTGGTGCACGTGGTGGAGGTGAGGTGTATTGTTACTATGGTCTTTATAAAGTTGAATCTATTTGGGAAAAAAAAGGGAAATTAGATTTTAAATTTTGTTTGGTTAGATTGCCTGATCAGAAATTTTTTGTTAAAGATATTTCCAATGGAAAAGAAGCTGTTCCTATATGTGTGGTTAATCGTATAGATTCTGGCAAGGTTCCATTTTTTCAATACATTACTCGTAACATTTATCCTGAATGGCTTTTCAATGATTCCTCTGTTGGGCGTAAATGTGTTGATCATTATTCTGATTCAATGAAATGTTCCTGTGCTCTTAAGAGTGGAGGGAAAATTACTAGAGTACAGAAAAATGAGATTAAATTTAATCTTCAAATTTTCAAGACTAAGGCGAAAGGATGGGGTGTGAGATCAGAGAATGCAATTCCTTCTGGACATTTTATTTGTGAGTATTTAGGTGAGATTATTGAAGATGAGGAAGCTTTGAAAATAGTTGATAATGATGAGTATTTATTTAATATTGGAAATTATATTCACAAGGTCTATAGTTCGTGGGAAGAGGATTCATATATGGTTGATAGTAAGGAGATGAGTTCATATCAACCCTTAGAAGCTTCAAGTGGTAAAGAGGCCATTGATGATCGATGTGATAGTGGTAGGTTTACGATTGATGCTGCTAAGTATGGTAACGTGGCAAGGTTTATCAACCATAGTTGTTCTCCCAATTTGTTTGCTCGAAATGTTCTTTACGATGATGATGATCTGAGGATTCCACACATCATGCTATATGCAGCTGAGAACATTCCGAGCATGAATGAGCTGACTTTGAATTACAATTACAAGATCGATCAAGTGATTGATTCAAATGGAAATTTAAAGACGAAAGCATGTTATTGTGGAGCTTCTGAATGCATCCGTCGACTGTATTGA
Protein sequence

>MsaT037044.1

MTKTKPSSSTTNHHHKPFQVSNRNSTDRALLFKNVSTRRVLSKVQRNTETKETPIKKLHLKRRCEDATSSNKSSLTSDDSRRKKVKTTLGNFRALCGKLGKELTADALPIEVSRILKNRGKFTHDGKKIIGNVPGIEVGDKFQYWQELNVVGLHRQNLSLIDYVIKDDNLIATSIVSCHFDDMDDTKVFVYTGEGGNVINSGECDKGNLALMNSFAVKNPIRVIIKFNSKNGGGARGGGEVYCYYGLYKVESIWEKKGKLDFKFCLVRLPDQKFFVKDISNGKEAVPICVVNRIDSGKVPFFQYITRNIYPEWLFNDSSVGRKCVDHYSDSMKCSCALKSGGKITRVQKNEIKFNLQIFKTKAKGWGVRSENAIPSGHFICEYLGEIIEDEEALKIVDNDEYLFNIGNYIHKVYSSWEEDSYMVDSKEMSSYQPLEASSGKEAIDDRCDSGRFTIDAAKYGNVARFINHSCSPNLFARNVLYDDDDLRIPHIMLYAAENIPSMNELTLNYNYKIDQVIDSNGNLKTKACYCGASECIRRLY*