AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037045

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037045 MtrunA17_Chr7g0232811 74.007 277 17 2 144 369 267 539 2.59e-139 407
MsaG037045 MtrunA17_Chr7g0232811 70.936 203 15 3 1 162 1 200 2.05e-84 266
MsaG037045 MtrunA17_Chr7g0232841 71.583 278 23 3 144 369 255 528 1.02e-131 387
MsaG037045 MtrunA17_Chr7g0232841 66.495 194 27 3 1 162 1 188 1.04e-72 235
MsaG037045 MtrunA17_Chr7g0232981 71.483 263 34 2 144 369 258 516 2.27e-125 370
MsaG037045 MtrunA17_Chr7g0232981 70.769 195 15 2 1 156 1 192 1.04e-83 263
MsaG037045 MtrunA17_Chr7g0232861 70.370 189 17 1 7 156 3 191 1.26e-83 254
MsaG037045 MtrunA17_Chr7g0232761 59.908 217 29 3 1 162 1 214 9.67e-72 225
MsaG037045 MtrunA17_Chr7g0232751 95.327 107 5 0 263 369 13 119 4.17e-70 215
MsaG037045 MtrunA17_Chr4g0003511 44.086 279 90 3 157 369 774 1052 2.58e-64 221
MsaG037045 MtrunA17_Chr4g0003501 44.043 277 91 3 157 369 679 955 9.24e-64 219
MsaG037045 MtrunA17_Chr4g0003501 27.941 136 85 3 23 156 450 574 7.36e-11 63.9
MsaG037045 MtrunA17_Chr5g0399801 44.803 279 88 4 157 369 811 1089 2.07e-63 218
MsaG037045 MtrunA17_Chr1g0170651 35.019 257 118 5 157 369 295 546 2.07e-40 150
MsaG037045 MtrunA17_Chr7g0252211 33.333 291 116 6 157 369 467 757 3.09e-37 142
MsaG037045 MtrunA17_Chr6g0476111 32.990 291 117 6 157 369 404 694 3.17e-36 139
MsaG037045 MtrunA17_Chr6g0476121 31.959 291 120 5 157 369 353 643 6.28e-35 135
MsaG037045 MtrunA17_Chr8g0369301 30.120 249 107 4 157 338 487 735 3.54e-29 119
MsaG037045 MtrunA17_Chr8g0368901 30.159 252 105 6 157 339 507 756 3.58e-29 119
MsaG037045 MtrunA17_Chr8g0368911 29.880 251 105 6 157 338 500 748 3.24e-27 113
MsaG037045 MtrunA17_Chr7g0232871 65.385 78 27 0 211 288 1 78 4.33e-27 103
MsaG037045 MtrunA17_Chr3g0080761 28.223 287 124 7 157 364 376 659 2.97e-26 110
MsaG037045 MtrunA17_Chr4g0075321 35.673 171 77 3 225 369 901 1064 6.80e-26 109
MsaG037045 MtrunA17_Chr8g0354821 28.223 287 123 8 156 364 402 683 5.38e-25 106
MsaG037045 MtrunA17_Chr1g0163051 28.906 256 115 4 157 345 396 651 6.92e-25 106
MsaG037045 MtrunA17_Chr6g0467421 27.244 312 146 8 103 338 256 562 8.22e-24 102
MsaG037045 MtrunA17_Chr5g0403241 35.673 171 77 5 225 369 1344 1507 1.31e-23 102
MsaG037045 MtrunA17_Chr3g0094881 25.954 262 117 6 151 338 363 621 5.65e-22 97.4
MsaG037045 MtrunA17_Chr8g0373821 35.976 164 73 5 225 364 378 533 2.49e-21 95.5
MsaG037045 MtrunA17_Chr2g0306961 28.743 167 78 2 213 341 301 464 2.02e-18 86.3
MsaG037045 MtrunA17_Chr1g0183321 32.778 180 88 7 212 364 568 741 1.87e-16 80.9
MsaG037045 MtrunA17_Chr1g0201311 34.932 146 65 8 236 364 87 219 4.36e-13 70.5
MsaG037045 MtrunA17_Chr2g0297191 27.044 159 85 6 222 364 1023 1166 3.88e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037045 AT2G35160.5 45.669 254 95 4 157 369 507 758 1.41e-65 221
MsaG037045 AT2G35160.2 45.669 254 95 4 157 369 543 794 2.22e-65 221
MsaG037045 AT2G35160.3 45.669 254 95 4 157 369 543 794 2.22e-65 221
MsaG037045 AT2G35160.1 45.669 254 95 4 157 369 543 794 2.22e-65 221
MsaG037045 AT2G35160.4 45.669 254 95 4 157 369 543 794 2.22e-65 221
MsaG037045 AT2G22740.2 41.197 284 94 7 157 369 509 790 3.17e-56 196
MsaG037045 AT2G22740.1 41.197 284 94 7 157 369 509 790 3.17e-56 196
MsaG037045 AT5G13960.2 32.414 290 119 5 157 369 201 490 4.47e-37 140
MsaG037045 AT5G13960.1 32.414 290 119 5 157 369 335 624 5.41e-37 141
MsaG037045 AT4G13460.1 29.921 254 105 6 157 339 386 637 4.39e-26 110
MsaG037045 AT4G13460.2 29.921 254 105 6 157 339 386 637 4.39e-26 110
MsaG037045 AT5G04940.2 27.857 280 128 9 157 364 388 665 4.72e-24 104
MsaG037045 AT5G04940.1 27.857 280 128 9 157 364 388 665 4.72e-24 104
MsaG037045 AT2G33290.1 28.788 264 116 7 157 352 388 647 5.10e-24 103
MsaG037045 AT2G33290.3 28.788 264 116 7 157 352 388 647 5.10e-24 103
MsaG037045 AT2G33290.4 28.788 264 116 7 157 352 388 647 5.10e-24 103
MsaG037045 AT2G33290.2 28.788 264 116 7 157 352 388 647 5.10e-24 103
MsaG037045 AT1G73100.1 26.523 279 134 6 157 364 386 664 5.78e-23 100
MsaG037045 AT2G24740.1 28.623 276 125 9 156 364 480 750 7.73e-22 97.8
MsaG037045 AT2G23740.1 28.720 289 121 11 157 364 1086 1370 6.21e-21 95.1
MsaG037045 AT2G23740.3 28.720 289 121 11 157 364 1093 1377 6.69e-21 95.1
MsaG037045 AT2G23740.6 28.720 289 121 11 157 364 1093 1377 6.69e-21 95.1
MsaG037045 AT2G23740.2 28.720 289 121 11 157 364 1093 1377 6.69e-21 95.1
MsaG037045 AT3G04380.6 30.811 185 91 7 212 367 257 433 5.77e-15 76.3
MsaG037045 AT3G04380.2 30.811 185 91 7 212 367 257 433 6.04e-15 76.3
MsaG037045 AT3G04380.5 30.811 185 91 7 212 367 241 417 6.80e-15 76.3
MsaG037045 AT3G04380.4 30.811 185 91 7 212 367 284 460 7.26e-15 76.3
MsaG037045 AT3G04380.3 30.811 185 91 7 212 367 268 444 7.86e-15 75.9
MsaG037045 AT3G04380.1 30.811 185 91 7 212 367 284 460 7.93e-15 76.3
MsaG037045 AT1G04050.2 28.333 180 93 7 212 364 548 718 7.71e-13 70.5
MsaG037045 AT1G04050.1 28.333 180 93 7 212 364 548 718 7.71e-13 70.5
MsaG037045 AT1G04050.3 28.333 180 93 7 212 364 444 614 8.15e-13 70.1
MsaG037045 AT5G43990.8 28.729 181 96 6 212 367 463 635 1.68e-12 69.3
MsaG037045 AT5G43990.12 28.729 181 96 6 212 367 463 635 1.68e-12 69.3
MsaG037045 AT5G43990.6 28.729 181 96 6 212 367 463 635 1.68e-12 69.3
MsaG037045 AT5G43990.13 28.729 181 96 6 212 367 463 635 1.68e-12 69.3
MsaG037045 AT5G43990.7 28.729 181 96 6 212 367 443 615 1.82e-12 68.9
MsaG037045 AT5G43990.15 28.729 181 96 6 212 367 535 707 2.01e-12 68.9
MsaG037045 AT5G43990.10 28.729 181 96 6 212 367 535 707 2.01e-12 68.9
MsaG037045 AT5G43990.9 28.729 181 96 6 212 367 555 727 2.15e-12 68.9
MsaG037045 AT5G43990.2 28.729 181 96 6 212 367 555 727 2.15e-12 68.9
MsaG037045 AT5G43990.3 28.729 181 96 6 212 367 532 704 2.20e-12 68.9
MsaG037045 AT5G43990.11 28.729 181 96 6 212 367 532 704 2.20e-12 68.9
MsaG037045 AT5G43990.1 28.729 181 96 6 212 367 532 704 2.20e-12 68.9
MsaG037045 AT5G43990.4 28.729 181 96 6 212 367 532 704 2.20e-12 68.9
MsaG037045 AT5G43990.16 28.729 181 96 6 212 367 512 684 2.23e-12 68.9
MsaG037045 AT5G43990.5 28.729 181 96 6 212 367 512 684 2.23e-12 68.9
MsaG037045 AT5G43990.17 28.729 181 96 6 212 367 512 684 2.23e-12 68.9
MsaG037045 AT5G43990.14 28.729 181 96 6 212 367 512 684 2.23e-12 68.9
MsaG037045 AT3G61740.2 31.973 147 77 5 233 364 839 977 3.79e-11 65.1
MsaG037045 AT3G61740.1 31.973 147 77 5 233 364 875 1013 4.03e-11 65.1

Find 61 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATTTCGAGCAAACAAAT+TGG 0.261209 7:+50650689 None:intergenic
CAAAAGTGTTCGTTTATATA+GGG 0.274754 7:-50651551 MsaT037045.1:CDS
CGAGTAATGTATTGAAAATA+TGG 0.296319 7:+50651207 None:intergenic
CTTGAAATGGTTTGTGTTGT+GGG 0.304611 7:+50652071 None:intergenic
ACAATTGATGCTGCTAAGTA+TGG 0.305938 7:-50650747 MsaT037045.1:CDS
ACAAAAGTGTTCGTTTATAT+AGG 0.318564 7:-50651552 MsaT037045.1:CDS
TCAGAGAATGCAATTCCTTC+TGG 0.338329 7:-50650997 MsaT037045.1:CDS
GAATCATTGAAAAGCCATTC+AGG 0.342986 7:+50651174 None:intergenic
ACAAATTGGGAGAACAACTA+TGG 0.363494 7:+50650703 None:intergenic
TCTCATTGATTACGTGATAA+AGG 0.363505 7:-50651631 MsaT037045.1:CDS
TTTAAATGCAGTTTCTTGAT+TGG 0.364503 7:+50651947 None:intergenic
TTTCTTGATTGGTGTTTCCT+TGG 0.368837 7:+50651958 None:intergenic
CTCGTAACATTTATCCTGAA+TGG 0.372209 7:-50651188 MsaT037045.1:CDS
TCTTTCAGTAAAGTTCTTGT+TGG 0.404016 7:+50652045 None:intergenic
GTGGTTAATCGTATAGATTC+TGG 0.413281 7:-50651237 MsaT037045.1:CDS
CGTTGTGAAGATGCAACTTC+AGG 0.429661 7:-50651920 MsaT037045.1:CDS
ACATTTCGAGCAAACAAATT+GGG 0.449844 7:+50650690 None:intergenic
ATACGATTAACCACACATAT+AGG 0.451047 7:+50651246 None:intergenic
TGGCAAGAACTCAATGTTGT+TGG 0.454774 7:-50651675 MsaT037045.1:CDS
ACTTGAAATGGTTTGTGTTG+TGG 0.465435 7:+50652070 None:intergenic
TTCTTGTTGGAGACTTGAAA+TGG 0.467403 7:+50652058 None:intergenic
CCCTCCACTCTTAAGAGCAC+AGG 0.479558 7:+50651101 None:intergenic
GATCAAGTGATTGATTCAAA+TGG 0.480841 7:-50650564 MsaT037045.1:CDS
AACCTTTGACAATACTCTCC+TGG 0.482538 7:+50651997 None:intergenic
TTTCTTTCTTCTTGAATCAT+CGG 0.487327 7:+50651877 None:intergenic
CAAGACTAAGGCGAATGGAT+GGG 0.511054 7:-50651028 MsaT037045.1:CDS
CGTGCTCTCTGTGGAAAACT+CGG 0.520054 7:-50651830 MsaT037045.1:intron
TTTGTTAAAGATATTTCCAA+TGG 0.523563 7:-50651282 MsaT037045.1:intron
TCAAGACTAAGGCGAATGGA+TGG 0.527589 7:-50651029 MsaT037045.1:CDS
AAGACGAAAGCATGTTATTG+TGG 0.528019 7:-50650534 MsaT037045.1:CDS
TAGGTGACAAGTTTCAGTAT+TGG 0.528561 7:-50651695 MsaT037045.1:CDS
TCAAATAAACACTGATACCA+AGG 0.533734 7:-50651975 MsaT037045.1:CDS
AAGACTAAGGCGAATGGATG+GGG 0.539750 7:-50651027 MsaT037045.1:CDS
AGAAGCTTCAAGTGGTAAAG+AGG 0.540440 7:-50650802 MsaT037045.1:CDS
AAAAGTGTTCGTTTATATAG+GGG 0.558261 7:-50651550 MsaT037045.1:CDS
CTAAGTATGGTAACGTGGCA+AGG 0.558339 7:-50650734 MsaT037045.1:CDS
ATTGATGATCGATGTGATAG+TGG 0.558469 7:-50650777 MsaT037045.1:CDS
ACTATCACATCGATCATCAA+TGG 0.565161 7:+50650779 None:intergenic
TCCTGTGCTCTTAAGAGTGG+AGG 0.568183 7:-50651102 MsaT037045.1:CDS
TTCGTTTATATAGGGGAAGG+AGG 0.575866 7:-50651543 MsaT037045.1:intron
GTGTTCGTTTATATAGGGGA+AGG 0.596025 7:-50651546 MsaT037045.1:CDS
AAAGTCAGCTCATTCATGCT+CGG 0.596521 7:+50650606 None:intergenic
TCACAAATAAAATGTCCAGA+AGG 0.599161 7:+50650982 None:intergenic
TGTTCCTGTGCTCTTAAGAG+TGG 0.599372 7:-50651105 MsaT037045.1:CDS
TGTTCAAGAATGTAAGCACC+AGG 0.604626 7:-50652015 MsaT037045.1:CDS
TTTACGATGATGATGATCTG+AGG 0.605700 7:-50650665 MsaT037045.1:CDS
CCTGTGCTCTTAAGAGTGGA+GGG 0.608017 7:-50651101 MsaT037045.1:CDS
AATCTCATCAATACAGTCGA+CGG 0.610597 7:+50650496 None:intergenic
ATGATCGATGTGATAGTGGT+AGG 0.614728 7:-50650773 MsaT037045.1:CDS
CACCAGGAGAGTATTGTCAA+AGG 0.620919 7:-50651999 MsaT037045.1:CDS
GGTAACTTCCGTGCTCTCTG+TGG 0.631604 7:-50651839 MsaT037045.1:CDS
TAATCGTATAGATTCTGGCA+AGG 0.632162 7:-50651232 MsaT037045.1:CDS
TGGGTTCGTAGTGGAAGACG+AGG 0.634379 7:+50652090 None:intergenic
AGAAGTTGTTCCTATATGTG+TGG 0.636958 7:-50651256 MsaT037045.1:CDS
TTTGTGTTGTGGGTTCGTAG+TGG 0.641933 7:+50652081 None:intergenic
CAACCCTTAGAAGCTTCAAG+TGG 0.647600 7:-50650810 MsaT037045.1:intron
GAATCATCGGAAGTCAAAGA+CGG 0.649357 7:+50651890 None:intergenic
AACACATTTACGCCCAACAG+AGG 0.665095 7:+50651152 None:intergenic
GGGTTCGTAGTGGAAGACGA+GGG 0.690983 7:+50652091 None:intergenic
GCTGCATATAGCATGATGTG+TGG 0.717627 7:+50650639 None:intergenic
TGCTGCTAAGTATGGTAACG+TGG 0.728265 7:-50650739 MsaT037045.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50650503 50652128 50650503 ID=MsaG037045
Chr7 mRNA 50650503 50652128 50650503 ID=MsaT037045.1;Parent=MsaG037045
Chr7 exon 50650503 50650822 50650503 ID=MsaT037045.1.exon4;Parent=MsaT037045.1
Chr7 CDS 50650503 50650822 50650503 ID=cds.MsaT037045.1;Parent=MsaT037045.1
Chr7 exon 50650974 50651294 50650974 ID=MsaT037045.1.exon3;Parent=MsaT037045.1
Chr7 CDS 50650974 50651294 50650974 ID=cds.MsaT037045.1;Parent=MsaT037045.1
Chr7 exon 50651544 50651714 50651544 ID=MsaT037045.1.exon2;Parent=MsaT037045.1
Chr7 CDS 50651544 50651714 50651544 ID=cds.MsaT037045.1;Parent=MsaT037045.1
Chr7 exon 50651831 50652128 50651831 ID=MsaT037045.1.exon1;Parent=MsaT037045.1
Chr7 CDS 50651831 50652128 50651831 ID=cds.MsaT037045.1;Parent=MsaT037045.1
Gene Sequence

>MsaT037045.1

ATGACTAAGACGAAGCCCTCGTCTTCCACTACGAACCCACAACACAAACCATTTCAAGTCTCCAACAAGAACTTTACTGAAAGATCATTGTTGTTCAAGAATGTAAGCACCAGGAGAGTATTGTCAAAGGTTCAAATAAACACTGATACCAAGGAAACACCAATCAAGAAACTGCATTTAAAACGTCGTTGTGAAGATGCAACTTCAGGTAACAAGCCGTCTTTGACTTCCGATGATTCAAGAAGAAAGAAAGTAAAAACTACATTAGGTAACTTCCGTGCTCTCTGTGGAAAACTCGGTGACAAGTTTCAGTATTGGCAAGAACTCAATGTTGTTGGTCTTCATCGTCAGAATCTAAGTCTCATTGATTACGTGATAAAGGATGATAATTTAATTGCAACTAGCATTGTTTCTTGTCATTTTGACGATATGGATGATACAAAAGTGTTCGTTTATATAGGGGAAGGAGATATTTCCAATGGAAAAGAAGTTGTTCCTATATGTGTGGTTAATCGTATAGATTCTGGCAAGGTTCCATATTTTCAATACATTACTCGTAACATTTATCCTGAATGGCTTTTCAATGATTCCTCTGTTGGGCGTAAATGTGTTGATCATTATTCTGATTCAATGAAATGTTCCTGTGCTCTTAAGAGTGGAGGGAAAATTACTAGAGTAAAGAAAAATGAGATTAAATTTAATCTTCAAATTTTCAAGACTAAGGCGAATGGATGGGGTGTGAGATCAGAGAATGCAATTCCTTCTGGACATTTTATTTGTGAGTATTTAGAAGCTTCAAGTGGTAAAGAGGCCATTGATGATCGATGTGATAGTGGTAGGTTTACAATTGATGCTGCTAAGTATGGTAACGTGGCAAGGTTTATAAACCATAGTTGTTCTCCCAATTTGTTTGCTCGAAATGTTCTTTACGATGATGATGATCTGAGGATTCCACACATCATGCTATATGCAGCTGAGAACATTCCGAGCATGAATGAGCTGACTTTGAATTACAATTACAAGATCGATCAAGTGATTGATTCAAATGGAAATTTAAAGACGAAAGCATGTTATTGTGGAGCTTCTGAATGCATCCGTCGACTGTATTGA
Protein sequence

>MsaT037045.1

MTKTKPSSSTTNPQHKPFQVSNKNFTERSLLFKNVSTRRVLSKVQINTDTKETPIKKLHLKRRCEDATSGNKPSLTSDDSRRKKVKTTLGNFRALCGKLGDKFQYWQELNVVGLHRQNLSLIDYVIKDDNLIATSIVSCHFDDMDDTKVFVYIGEGDISNGKEVVPICVVNRIDSGKVPYFQYITRNIYPEWLFNDSSVGRKCVDHYSDSMKCSCALKSGGKITRVKKNEIKFNLQIFKTKANGWGVRSENAIPSGHFICEYLEASSGKEAIDDRCDSGRFTIDAAKYGNVARFINHSCSPNLFARNVLYDDDDLRIPHIMLYAAENIPSMNELTLNYNYKIDQVIDSNGNLKTKACYCGASECIRRLY*