AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037056

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037056 MtrunA17_Chr7g0232681 66.327 196 18 2 1 148 1 196 9.61e-87 251
MsaG037056 MtrunA17_Chr7g0232701 63.043 138 13 2 48 148 1 137 9.48e-53 164
MsaG037056 MtrunA17_Chr7g0232671 70.130 77 23 0 73 149 176 252 1.45e-33 118
MsaG037056 MtrunA17_Chr3g0145121 55.224 67 28 1 83 147 214 280 3.13e-19 82.0
MsaG037056 MtrunA17_Chr4g0035891 53.846 65 30 0 81 145 208 272 3.94e-19 81.6
MsaG037056 MtrunA17_Chr3g0140331 59.016 61 25 0 84 144 253 313 4.58e-19 82.0
MsaG037056 MtrunA17_Chr2g0326771 52.857 70 33 0 83 152 215 284 9.93e-19 80.5
MsaG037056 MtrunA17_Chr6g0461591 45.455 88 46 1 65 152 175 260 1.22e-18 79.7
MsaG037056 MtrunA17_Chr4g0028961 56.452 62 27 0 84 145 232 293 1.24e-18 80.5
MsaG037056 MtrunA17_Chr1g0199671 53.731 67 31 0 84 150 242 308 2.96e-18 79.7
MsaG037056 MtrunA17_Chr1g0146231 56.452 62 27 0 84 145 234 295 4.52e-18 79.3
MsaG037056 MtrunA17_Chr7g0253101 56.452 62 27 0 84 145 188 249 6.93e-18 77.8
MsaG037056 MtrunA17_Chr8g0340401 54.839 62 28 0 84 145 226 287 1.68e-17 77.8
MsaG037056 MtrunA17_Chr5g0404981 42.391 92 51 1 63 152 279 370 1.79e-17 77.8
MsaG037056 MtrunA17_Chr8g0372291 49.296 71 34 1 84 152 218 288 3.56e-17 76.3
MsaG037056 MtrunA17_Chr1g0213161 54.839 62 28 0 84 145 152 213 7.93e-17 74.3
MsaG037056 MtrunA17_Chr1g0169431 53.731 67 29 1 84 148 172 238 2.92e-16 73.2
MsaG037056 MtrunA17_Chr5g0395391 48.611 72 35 1 84 153 270 341 4.14e-15 70.9
MsaG037056 MtrunA17_Chr5g0395391 50.000 62 31 0 84 145 168 229 1.05e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037056 AT3G60530.1 56.452 62 27 0 84 145 158 219 3.61e-20 83.6
MsaG037056 AT5G25830.1 57.143 63 27 0 83 145 218 280 8.44e-20 84.3
MsaG037056 AT2G45050.1 54.839 62 28 0 84 145 179 240 1.05e-19 82.8
MsaG037056 AT4G36240.1 30.102 196 85 6 2 145 30 225 1.47e-19 82.0
MsaG037056 AT4G32890.1 56.452 62 27 0 84 145 197 258 4.54e-19 82.0
MsaG037056 AT3G54810.1 50.633 79 38 1 73 150 217 295 1.66e-18 80.5
MsaG037056 AT3G54810.2 50.633 79 38 1 73 150 217 295 1.66e-18 80.5
MsaG037056 AT4G34680.1 33.121 157 62 3 27 145 90 241 1.78e-18 79.7
MsaG037056 AT4G34680.2 33.121 157 62 3 27 145 90 241 1.78e-18 79.7
MsaG037056 AT1G08000.2 56.716 67 27 1 84 148 218 284 1.79e-18 80.5
MsaG037056 AT1G08000.1 56.716 67 27 1 84 148 218 284 1.79e-18 80.5
MsaG037056 AT2G28340.1 58.333 60 25 0 86 145 193 252 2.35e-18 79.7
MsaG037056 AT5G66320.2 53.968 63 29 0 83 145 248 310 2.91e-18 80.1
MsaG037056 AT5G66320.1 53.968 63 29 0 83 145 248 310 2.91e-18 80.1
MsaG037056 AT3G45170.1 51.471 68 31 1 86 151 117 184 9.51e-18 76.6
MsaG037056 AT1G08010.2 53.226 62 29 0 84 145 220 281 3.62e-17 76.6
MsaG037056 AT1G08010.1 53.226 62 29 0 84 145 220 281 3.62e-17 76.6
MsaG037056 AT1G08010.3 53.226 62 29 0 84 145 220 281 3.62e-17 76.6
MsaG037056 AT3G51080.1 51.429 70 32 1 84 151 221 290 4.18e-17 76.6
MsaG037056 AT3G24050.1 53.731 67 29 1 84 148 194 260 1.03e-16 75.1

Find 37 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGGAGGTTGGGTCCTT+TGG 0.187764 7:-50906941 MsaT037056.1:CDS
GTTCTGGCAATCCTCATCTT+TGG 0.213267 7:-50907660 MsaT037056.1:CDS
TTTAGAAAAGGTCATCAAAT+TGG 0.233940 7:-50907076 MsaT037056.1:CDS
CATCAAATTGGATGAATTAA+TGG 0.255209 7:-50907064 MsaT037056.1:CDS
ACAGGAGGCACTAGAAAATT+TGG 0.257280 7:-50907133 MsaT037056.1:CDS
CAATGGAGGTTGGGTCCTTT+GGG 0.260138 7:-50906940 MsaT037056.1:CDS
AAGCTAAATGGATTGGTTAA+AGG 0.267058 7:-50906880 MsaT037056.1:CDS
CAAATTCTCTGATGATGATT+AGG 0.271705 7:+50906991 None:intergenic
TTCTGGCAATCCTCATCTTT+GGG 0.283554 7:-50907659 MsaT037056.1:intron
CCTCCATTGAGTAGTTTCTT+TGG 0.310305 7:+50906954 None:intergenic
GGATTACGATACAAGCTAAA+TGG 0.317668 7:-50906892 MsaT037056.1:CDS
AGCTAAATGGATTGGTTAAA+GGG 0.347180 7:-50906879 MsaT037056.1:CDS
AAATTCTCTGATGATGATTA+GGG 0.356028 7:+50906992 None:intergenic
AACATGAAGTGGGTCAGTTC+TGG 0.366216 7:-50907676 MsaT037056.1:CDS
ATCAAATTGGATGAATTAAT+GGG 0.418542 7:-50907063 MsaT037056.1:CDS
TCAAAAGCTGGACTTGCCTT+AGG 0.449589 7:+50906850 None:intergenic
CTTGTATCAATATTGAAAGA+TGG 0.486236 7:+50907018 None:intergenic
GAAACTACTCAATGGAGGTT+GGG 0.488791 7:-50906949 MsaT037056.1:CDS
TCATTCAAATTTACACAAGA+AGG 0.495479 7:-50906818 MsaT037056.1:CDS
ACGATACAAGCTAAATGGAT+TGG 0.515501 7:-50906887 MsaT037056.1:CDS
ACACAAGAAGGCGATGAGAA+AGG 0.520810 7:-50906806 MsaT037056.1:CDS
AGAAACTACTCAATGGAGGT+TGG 0.521850 7:-50906950 MsaT037056.1:CDS
TGACATGGAGAATTTAGAAA+AGG 0.524637 7:-50907088 MsaT037056.1:CDS
AATGGAGGTTGGGTCCTTTG+GGG 0.535179 7:-50906939 MsaT037056.1:CDS
TGATTTCTGATATCAAAAGC+TGG 0.547788 7:+50906838 None:intergenic
GGTTAAAGGGTATAGACCTA+AGG 0.562082 7:-50906866 MsaT037056.1:CDS
AGATTTGAGTAAACATGAAG+TGG 0.567173 7:-50907687 MsaT037056.1:CDS
AAGAAGGCGATGAGAAAGGA+AGG 0.572140 7:-50906802 MsaT037056.1:CDS
AGACCAAAGAAACTACTCAA+TGG 0.572896 7:-50906957 MsaT037056.1:CDS
GGATCTTGATTATATCCCAC+AGG 0.596434 7:-50907151 MsaT037056.1:CDS
CACAAAGTATTCTTCCCCAA+AGG 0.604152 7:+50906925 None:intergenic
AATACTTTGTGCAATGCATG+TGG 0.615784 7:-50906913 MsaT037056.1:CDS
AGGCACTAGAAAATTTGGAA+TGG 0.632247 7:-50907128 MsaT037056.1:CDS
ATAATACTAACCCAAAGATG+AGG 0.642509 7:+50907649 None:intergenic
CCAAAGAAACTACTCAATGG+AGG 0.662722 7:-50906954 MsaT037056.1:CDS
GATTTGAGTAAACATGAAGT+GGG 0.670715 7:-50907686 MsaT037056.1:CDS
TCTTGATTATATCCCACAGG+AGG 0.714474 7:-50907148 MsaT037056.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 50906786 50907732 50906786 ID=MsaG037056
Chr7 mRNA 50906786 50907732 50906786 ID=MsaT037056.1;Parent=MsaG037056
Chr7 exon 50906786 50907174 50906786 ID=MsaT037056.1.exon2;Parent=MsaT037056.1
Chr7 CDS 50906786 50907174 50906786 ID=cds.MsaT037056.1;Parent=MsaT037056.1
Chr7 exon 50907660 50907732 50907660 ID=MsaT037056.1.exon1;Parent=MsaT037056.1
Chr7 CDS 50907660 50907732 50907660 ID=cds.MsaT037056.1;Parent=MsaT037056.1
Gene Sequence

>MsaT037056.1

ATGGCTAATCAGAGAGAAGAACAAGATTTGAGTAAACATGAAGTGGGTCAGTTCTGGCAATCCTCATCTTTGGGGGATCTTGATTATATCCCACAGGAGGCACTAGAAAATTTGGAATGGTACACTACTTTTTGGGATGACATGGAGAATTTAGAAAAGGTCATCAAATTGGATGAATTAATGGGTAAGAGAAAAACACAAGAAAATCCATCTTTCAATATTGATACAAGTGTCCCTAATCATCATCAGAGAATTTGCAGCGATTGTGAGACCAAAGAAACTACTCAATGGAGGTTGGGTCCTTTGGGGAAGAATACTTTGTGCAATGCATGTGGATTACGATACAAGCTAAATGGATTGGTTAAAGGGTATAGACCTAAGGCAAGTCCAGCTTTTGATATCAGAAATCATTCAAATTTACACAAGAAGGCGATGAGAAAGGAAGGAAAAACAATCATTTAG
Protein sequence

>MsaT037056.1

MANQREEQDLSKHEVGQFWQSSSLGDLDYIPQEALENLEWYTTFWDDMENLEKVIKLDELMGKRKTQENPSFNIDTSVPNHHQRICSDCETKETTQWRLGPLGKNTLCNACGLRYKLNGLVKGYRPKASPAFDIRNHSNLHKKAMRKEGKTII*