Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037109 | XP_020230309.1 | 51.220 | 246 | 61 | 6 | 1 | 191 | 1 | 242 | 1.47e-70 | 227 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037109 | sp|Q8GX84|C3H1_ARATH | 44.770 | 239 | 77 | 4 | 5 | 191 | 14 | 249 | 1.08e-57 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037109 | tr|A0A445EFQ4|A0A445EFQ4_ARAHY | 51.626 | 246 | 60 | 6 | 1 | 191 | 1 | 242 | 5.10e-69 | 223 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG037109 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG037109 | MsaG037110 | 0.862053 | 1.316255e-63 | 1.106970e-60 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037109 | MtrunA17_Chr7g0269781 | 56.098 | 246 | 47 | 7 | 1 | 191 | 1 | 240 | 1.65e-69 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037109 | AT1G01350.1 | 44.770 | 239 | 77 | 4 | 5 | 191 | 14 | 249 | 1.10e-58 | 187 |
| MsaG037109 | AT5G06420.1 | 45.415 | 229 | 73 | 3 | 12 | 191 | 59 | 284 | 1.52e-58 | 188 |
| MsaG037109 | AT5G06420.2 | 45.415 | 229 | 73 | 3 | 12 | 191 | 59 | 284 | 1.52e-58 | 188 |
Find 41 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGAAAGACAAGTTTCT+TGG | 0.272408 | 7:-51877218 | None:intergenic |
| AACTACCTTCTTGTTGAGTT+TGG | 0.337597 | 7:-51876906 | None:intergenic |
| ATTTGCAGCAGAAGCAGGTT+TGG | 0.390936 | 7:-51876881 | None:intergenic |
| TTGGATGCTTCATTCGGATT+CGG | 0.394839 | 7:-51877199 | None:intergenic |
| TTGCCAGCCGGACTTGTAAT+CGG | 0.412299 | 7:-51877476 | None:intergenic |
| TCAGCGCATCCTCCTCTTCT+CGG | 0.426742 | 7:-51877521 | None:intergenic |
| CGACGTATGCAAAGACTTCA+AGG | 0.435111 | 7:+51877402 | MsaT037109.1:CDS |
| AGTTTCTTGGATGCTTCATT+CGG | 0.444837 | 7:-51877205 | None:intergenic |
| ATCGCCGTAGCCACAATATC+CGG | 0.451109 | 7:-51877428 | None:intergenic |
| TTGAAGTCTTTGCATACGTC+GGG | 0.464121 | 7:-51877400 | None:intergenic |
| TGGTGATTTGCAGCAGAAGC+AGG | 0.471446 | 7:-51876886 | None:intergenic |
| TAGCAAAGCAACATCAACTT+TGG | 0.480022 | 7:+51877273 | MsaT037109.1:CDS |
| CTTGAAGTCTTTGCATACGT+CGG | 0.492059 | 7:-51877401 | None:intergenic |
| AGCCGGACTTGTAATCGGTT+CGG | 0.494983 | 7:-51877471 | None:intergenic |
| GAAACTCTAATATTAGTTGA+AGG | 0.497420 | 7:-51877358 | None:intergenic |
| GCTGGCAATTAGAGAAAGAA+TGG | 0.512017 | 7:+51877491 | MsaT037109.1:CDS |
| GACCGAACCGATTACAAGTC+CGG | 0.518417 | 7:+51877469 | MsaT037109.1:CDS |
| TTCTTTCTCTAATTGCCAGC+CGG | 0.522931 | 7:-51877488 | None:intergenic |
| ACAAGAATAAGAAGAATTTG+AGG | 0.528415 | 7:+51877053 | MsaT037109.1:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.528595 | 7:+51877409 | MsaT037109.1:CDS |
| AGAAGAGGAGGATGCGCTGA+TGG | 0.539632 | 7:+51877524 | MsaT037109.1:CDS |
| TATAGAGAACGAGGATAATG+AGG | 0.545622 | 7:+51877087 | MsaT037109.1:CDS |
| CTCTAATATTAGTTGAAGGT+CGG | 0.563651 | 7:-51877354 | None:intergenic |
| TTCAAGGAGACCGGATATTG+TGG | 0.565573 | 7:+51877418 | MsaT037109.1:CDS |
| AGTATCTGGTTTCTTTAACA+AGG | 0.571433 | 7:+51877024 | MsaT037109.1:intron |
| ATTCGGATTCGGTTTAGCAG+TGG | 0.576740 | 7:-51877188 | None:intergenic |
| AGAATAAGAAGAATTTGAGG+AGG | 0.578892 | 7:+51877056 | MsaT037109.1:CDS |
| GAGACCGGATATTGTGGCTA+CGG | 0.581464 | 7:+51877424 | MsaT037109.1:CDS |
| GGAGAGGACCATGTGACCCA+TGG | 0.582366 | 7:-51877333 | None:intergenic |
| GAGATACGAGTTCCATCCAT+GGG | 0.582633 | 7:+51877317 | MsaT037109.1:CDS |
| GGAATGAAGCCGAGAAGAGG+AGG | 0.588901 | 7:+51877512 | MsaT037109.1:CDS |
| GAACCGATTACAAGTCCGGC+TGG | 0.593400 | 7:+51877473 | MsaT037109.1:CDS |
| AATGGAATGAAGCCGAGAAG+AGG | 0.595307 | 7:+51877509 | MsaT037109.1:CDS |
| TATCAGTATGGAAGATTCTG+AGG | 0.603098 | 7:+51876858 | None:intergenic |
| AGTTCCATCCATGGGTCACA+TGG | 0.604452 | 7:+51877325 | MsaT037109.1:CDS |
| AGAGATACGAGTTCCATCCA+TGG | 0.609687 | 7:+51877316 | MsaT037109.1:CDS |
| AGGACCATGTGACCCATGGA+TGG | 0.614584 | 7:-51877329 | None:intergenic |
| AATCACCAAACTCAACAAGA+AGG | 0.618287 | 7:+51876901 | MsaT037109.1:CDS |
| TGAGGATGACTCAAACATTG+TGG | 0.620048 | 7:+51877105 | MsaT037109.1:CDS |
| ATATTAGTTGAAGGTCGGAG+AGG | 0.706689 | 7:-51877349 | None:intergenic |
| GAAGCGTACTATAGAGAACG+AGG | 0.706962 | 7:+51877078 | MsaT037109.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 51876865 | 51877543 | 51876865 | ID=MsaG037109 |
| Chr7 | mRNA | 51876865 | 51877543 | 51876865 | ID=MsaT037109.1;Parent=MsaG037109 |
| Chr7 | exon | 51876865 | 51876922 | 51876865 | ID=MsaT037109.1.exon1;Parent=MsaT037109.1 |
| Chr7 | CDS | 51876865 | 51876922 | 51876865 | ID=cds.MsaT037109.1;Parent=MsaT037109.1 |
| Chr7 | exon | 51877026 | 51877543 | 51877026 | ID=MsaT037109.1.exon2;Parent=MsaT037109.1 |
| Chr7 | CDS | 51877026 | 51877543 | 51877026 | ID=cds.MsaT037109.1;Parent=MsaT037109.1 |
| Gene Sequence |
| Protein sequence |
