Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037116 | RHN67051.1 | 81.935 | 155 | 27 | 1 | 1 | 154 | 1 | 155 | 1.07e-85 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037116 | tr|A0A396IQQ6|A0A396IQQ6_MEDTR | 81.935 | 155 | 27 | 1 | 1 | 154 | 1 | 155 | 5.12e-86 | 258 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG037116 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037116 | MtrunA17_Chr3g0098461 | 81.935 | 155 | 27 | 1 | 1 | 154 | 1 | 155 | 9.86e-90 | 258 |
| MsaG037116 | MtrunA17_Chr0c05g0492161 | 71.074 | 121 | 34 | 1 | 1 | 120 | 1 | 121 | 5.50e-56 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 40 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCGTCAGTGTTGGTCTTC+GGG | 0.301799 | 7:-52073015 | None:intergenic |
| GGTTTCATCGTCTTTGATAT+TGG | 0.310497 | 7:+52072724 | MsaT037116.1:CDS |
| AACCTTGTCCAAGTCCATTT+CGG | 0.354048 | 7:-52072842 | None:intergenic |
| AATGCGTCAGTGTTGGTCTT+CGG | 0.376239 | 7:-52073016 | None:intergenic |
| ACTTGTGACCATGGCCGAAA+TGG | 0.380600 | 7:+52072828 | MsaT037116.1:CDS |
| CATCCACAAGAATCTTAAAT+AGG | 0.405177 | 7:-52072615 | None:intergenic |
| AACCCTATCTTTCGGAGAAA+AGG | 0.415290 | 7:+52072793 | MsaT037116.1:CDS |
| GTGAAGAGCAATAATATTGA+TGG | 0.429245 | 7:+52073073 | MsaT037116.1:CDS |
| TGACGTGGATGCTAAAAGTT+TGG | 0.430756 | 7:+52072648 | MsaT037116.1:CDS |
| TCTTCGTTAACCCTATCTTT+CGG | 0.431368 | 7:+52072785 | MsaT037116.1:CDS |
| GTCCAAGTCCATTTCGGCCA+TGG | 0.446272 | 7:-52072836 | None:intergenic |
| GACCATGGCCGAAATGGACT+TGG | 0.499061 | 7:+52072834 | MsaT037116.1:CDS |
| GATCTGAAGCATACTGGTTA+TGG | 0.504056 | 7:+52072748 | MsaT037116.1:CDS |
| GGACCTATTTAAGATTCTTG+TGG | 0.505182 | 7:+52072612 | MsaT037116.1:CDS |
| GAGCAATAATATTGATGGGA+AGG | 0.524325 | 7:+52073078 | MsaT037116.1:CDS |
| ATTGGTGATCTGAAGCATAC+TGG | 0.528676 | 7:+52072742 | MsaT037116.1:CDS |
| GTCGCCGAGGATAAACTCCT+AGG | 0.541691 | 7:+52072691 | MsaT037116.1:CDS |
| GGCCGAAATGGACTTGGACA+AGG | 0.545609 | 7:+52072840 | MsaT037116.1:CDS |
| TGAAGAGCAATAATATTGAT+GGG | 0.562579 | 7:+52073074 | MsaT037116.1:CDS |
| GCATCTCTTACGACCACATG+TGG | 0.566352 | 7:-52072580 | None:intergenic |
| AGAATCTTAAATAGGTCCAC+AGG | 0.578847 | 7:-52072607 | None:intergenic |
| AAACTCCTAGGGTTTATCCT+CGG | 0.580594 | 7:+52072703 | MsaT037116.1:CDS |
| ATCATGACGAAGAAGCTCGA+GGG | 0.582010 | 7:+52073115 | MsaT037116.1:CDS |
| CGCTACTCGACTTGTGACCA+TGG | 0.583316 | 7:+52072819 | MsaT037116.1:CDS |
| TAAGAGTAATGCGTCAGTGT+TGG | 0.594559 | 7:-52073023 | None:intergenic |
| AAAGATAGGGTTAACGAAGA+GGG | 0.602204 | 7:-52072782 | None:intergenic |
| TCGCCGAGGATAAACTCCTA+GGG | 0.604901 | 7:+52072692 | MsaT037116.1:CDS |
| AAACCCTAGGAGTTTATCCT+CGG | 0.607628 | 7:-52072695 | None:intergenic |
| GAAAGATAGGGTTAACGAAG+AGG | 0.620492 | 7:-52072783 | None:intergenic |
| GTCAGTGTTGGTCTTCGGGA+CGG | 0.627712 | 7:-52073011 | None:intergenic |
| ATCTCTTACGACCACATGTG+GGG | 0.629012 | 7:-52072578 | None:intergenic |
| TGAAACCGAGGATAAACCCT+AGG | 0.631949 | 7:-52072708 | None:intergenic |
| CATCTCTTACGACCACATGT+GGG | 0.632600 | 7:-52072579 | None:intergenic |
| CGTAAGAGATGCAACACCTG+TGG | 0.632733 | 7:+52072591 | MsaT037116.1:CDS |
| GATGCTAAAAGTTTGGCGAA+TGG | 0.635951 | 7:+52072655 | MsaT037116.1:CDS |
| CATCATGACGAAGAAGCTCG+AGG | 0.646448 | 7:+52073114 | MsaT037116.1:CDS |
| TCATGACGAAGAAGCTCGAG+GGG | 0.655307 | 7:+52073116 | MsaT037116.1:CDS |
| AAAGATGGAGTCCCCACATG+TGG | 0.657747 | 7:+52072567 | None:intergenic |
| TATCAAAGACGATGAAACCG+AGG | 0.669702 | 7:-52072720 | None:intergenic |
| TAAGATTCTTCAAGTCGCCG+AGG | 0.715340 | 7:+52072678 | MsaT037116.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 52072571 | 52073159 | 52072571 | ID=MsaG037116 |
| Chr7 | mRNA | 52072571 | 52073159 | 52072571 | ID=MsaT037116.1;Parent=MsaG037116 |
| Chr7 | exon | 52072571 | 52072861 | 52072571 | ID=MsaT037116.1.exon1;Parent=MsaT037116.1 |
| Chr7 | CDS | 52072571 | 52072861 | 52072571 | ID=cds.MsaT037116.1;Parent=MsaT037116.1 |
| Chr7 | exon | 52072986 | 52073159 | 52072986 | ID=MsaT037116.1.exon2;Parent=MsaT037116.1 |
| Chr7 | CDS | 52072986 | 52073159 | 52072986 | ID=cds.MsaT037116.1;Parent=MsaT037116.1 |
| Gene Sequence |
| Protein sequence |
