AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037150 MtrunA17_Chr7g0233521 89.785 186 19 0 28 213 1 186 5.46e-123 350
MsaG037150 MtrunA17_Chr7g0233541 75.743 202 32 3 28 213 1 201 2.78e-103 300
MsaG037150 MtrunA17_Chr7g0233561 65.351 228 63 3 1 213 6 232 8.60e-99 290
MsaG037150 MtrunA17_Chr7g0233571 63.721 215 61 1 5 202 2 216 2.16e-97 283
MsaG037150 MtrunA17_Chr3g0131891 63.596 228 59 5 1 213 7 225 4.96e-92 273
MsaG037150 MtrunA17_Chr7g0233531 60.000 200 51 4 28 213 1 185 2.64e-71 218
MsaG037150 MtrunA17_Chr8g0390841 35.294 238 127 5 11 223 37 272 1.75e-37 136
MsaG037150 MtrunA17_Chr3g0103191 31.944 216 108 6 6 201 5 201 5.98e-31 114
MsaG037150 MtrunA17_Chr3g0103181 34.685 222 107 8 6 210 5 205 6.93e-31 114
MsaG037150 MtrunA17_Chr3g0103151 49.057 106 52 1 6 111 5 108 1.82e-30 110
MsaG037150 MtrunA17_Chr1g0208771 31.858 226 118 6 29 224 13 232 3.55e-30 116
MsaG037150 MtrunA17_Chr1g0163071 31.602 231 123 6 28 224 1 230 1.10e-29 112
MsaG037150 MtrunA17_Chr5g0419201 46.465 99 53 0 13 111 16 114 9.68e-29 107
MsaG037150 MtrunA17_Chr6g0467351 38.356 146 84 2 11 155 13 153 1.32e-24 97.1
MsaG037150 MtrunA17_Chr1g0163251 34.123 211 116 9 13 213 9 206 5.21e-24 98.6
MsaG037150 MtrunA17_Chr1g0163181 32.143 196 114 5 22 200 14 207 1.37e-23 97.8
MsaG037150 MtrunA17_Chr4g0067431 45.794 107 57 1 11 116 13 119 9.44e-23 94.0
MsaG037150 MtrunA17_Chr1g0163231 30.222 225 121 6 10 220 6 208 2.38e-22 92.4
MsaG037150 MtrunA17_Chr1g0163101 38.298 141 75 3 9 149 13 141 2.63e-22 89.7
MsaG037150 MtrunA17_Chr1g0163261 32.895 228 111 8 10 217 6 211 8.32e-22 91.3
MsaG037150 MtrunA17_Chr1g0171611 29.954 217 120 4 10 220 6 196 1.34e-21 90.5
MsaG037150 MtrunA17_Chr4g0033961 40.000 105 61 1 13 115 6 110 4.30e-21 90.5
MsaG037150 MtrunA17_Chr3g0103171 44.578 83 46 0 29 111 6 88 5.48e-21 88.2
MsaG037150 MtrunA17_Chr1g0162441 38.384 99 61 0 13 111 7 105 2.08e-20 88.6
MsaG037150 MtrunA17_Chr1g0163191 45.556 90 47 1 22 111 14 101 2.96e-20 84.0
MsaG037150 MtrunA17_Chr1g0163241 46.988 83 42 1 29 111 23 103 3.37e-19 81.3
MsaG037150 MtrunA17_Chr3g0077411 33.981 103 68 0 12 114 24 126 3.86e-19 85.5
MsaG037150 MtrunA17_Chr3g0077411 28.141 199 117 4 11 205 260 436 1.57e-18 83.6
MsaG037150 MtrunA17_Chr1g0163061 42.857 105 59 1 11 114 13 117 4.22e-19 83.2
MsaG037150 MtrunA17_Chr3g0077461 35.088 114 74 0 12 125 236 349 8.40e-19 84.3
MsaG037150 MtrunA17_Chr3g0077461 30.769 117 69 2 29 145 23 127 2.99e-16 77.0
MsaG037150 MtrunA17_Chr1g0163131 35.526 152 82 4 6 157 11 146 1.29e-18 82.4
MsaG037150 MtrunA17_Chr1g0163121 39.024 123 62 3 27 149 9 118 1.60e-18 79.0
MsaG037150 MtrunA17_Chr1g0163221 39.450 109 65 1 5 112 3 111 4.24e-18 81.6
MsaG037150 MtrunA17_Chr1g0163201 32.143 196 119 5 29 213 19 211 5.43e-18 81.6
MsaG037150 MtrunA17_Chr3g0077451 32.456 114 68 3 27 140 10 114 4.16e-16 74.7
MsaG037150 MtrunA17_Chr4g0009491 31.579 114 78 0 12 125 224 337 5.42e-16 76.3
MsaG037150 MtrunA17_Chr4g0009491 32.955 88 59 0 27 114 11 98 8.99e-15 72.4
MsaG037150 MtrunA17_Chr5g0429931 27.368 190 108 5 29 210 11 178 9.48e-16 73.9
MsaG037150 MtrunA17_Chr1g0163141 34.646 127 78 3 27 153 9 130 1.05e-15 72.4
MsaG037150 MtrunA17_Chr1g0162411 36.364 99 61 1 13 111 7 103 1.09e-15 75.1
MsaG037150 MtrunA17_Chr3g0077381 28.814 118 79 1 27 139 34 151 1.30e-15 75.1
MsaG037150 MtrunA17_Chr3g0077381 30.702 114 79 0 12 125 246 359 7.93e-15 72.8
MsaG037150 MtrunA17_Chr1g0162161 34.426 122 67 3 28 149 3 111 8.12e-15 69.7
MsaG037150 MtrunA17_Chr1g0154101 26.289 194 120 4 11 183 182 373 1.37e-14 72.4
MsaG037150 MtrunA17_Chr1g0154101 31.333 150 92 3 11 154 23 167 2.75e-14 71.6
MsaG037150 MtrunA17_Chr1g0154131 28.311 219 136 9 11 213 182 395 2.12e-14 71.6
MsaG037150 MtrunA17_Chr3g0077361 29.060 117 78 2 12 127 39 151 2.90e-14 71.2
MsaG037150 MtrunA17_Chr1g0163081 36.154 130 68 5 28 157 5 119 3.11e-14 67.4
MsaG037150 MtrunA17_Chr6g0476181 45.238 84 43 2 28 111 1 81 3.34e-14 67.8
MsaG037150 MtrunA17_Chr1g0154161 24.638 207 152 2 11 213 28 234 1.29e-13 68.9
MsaG037150 MtrunA17_Chr1g0154211 33.673 98 64 1 6 103 16 112 6.53e-13 67.0
MsaG037150 MtrunA17_Chr1g0154151 30.769 169 85 3 16 184 14 150 7.53e-13 66.6
MsaG037150 MtrunA17_Chr1g0154251 33.333 93 61 1 11 103 25 116 7.97e-13 67.0
MsaG037150 MtrunA17_Chr1g0154171 25.521 192 134 4 6 189 16 206 2.15e-12 65.5
MsaG037150 MtrunA17_Chr1g0154111 25.279 269 142 7 11 220 24 292 2.54e-12 65.5
MsaG037150 MtrunA17_Chr1g0154071 30.159 126 82 3 14 134 157 281 2.63e-12 65.5
MsaG037150 MtrunA17_Chr1g0154191 31.633 98 67 0 6 103 15 112 5.86e-12 64.3
MsaG037150 MtrunA17_Chr1g0154241 31.959 97 65 1 11 107 20 115 1.06e-11 62.8
MsaG037150 MtrunA17_Chr1g0163161 35.043 117 62 4 41 157 1 103 4.84e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037150 AT4G01580.1 35.075 134 82 1 13 141 30 163 5.82e-23 92.0
MsaG037150 AT3G18990.1 43.434 99 56 0 13 111 6 104 7.69e-23 95.1
MsaG037150 AT3G18990.2 43.434 99 56 0 13 111 6 104 1.12e-22 95.1
MsaG037150 AT1G49475.1 44.118 102 56 1 13 114 34 134 1.35e-22 91.3
MsaG037150 AT3G18960.2 39.423 104 63 0 13 116 30 133 1.14e-21 89.0
MsaG037150 AT3G18960.1 39.423 104 63 0 13 116 30 133 1.49e-21 89.0
MsaG037150 AT3G18960.3 42.045 88 51 0 29 116 9 96 6.69e-18 78.6

Find 33 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATGGAAATTAAATT+TGG 0.194062 7:+52573253 MsaT037150.1:CDS
TATTTCTCCACAAACTTTCT+TGG 0.217927 7:-52573185 None:intergenic
AGAAAGTTTGTGGAGAAATA+TGG 0.293086 7:+52573188 MsaT037150.1:CDS
CGTGGAACACCGATTTCTTA+TGG 0.302917 7:+52573762 MsaT037150.1:CDS
GAATGGATAATTTATCTCTA+AGG 0.305393 7:-52573414 None:intergenic
CCAAAGAGTTGCAGCTAAAA+AGG 0.311285 7:+52573436 MsaT037150.1:CDS
TTCCATCAACCACGTGAAAT+AGG 0.327923 7:+52573500 MsaT037150.1:CDS
AGACTCCAAATGGTGCAAAA+TGG 0.337543 7:+52573240 MsaT037150.1:CDS
GGCAAGGTATGGTTTGAAAA+AGG 0.397029 7:+52573287 MsaT037150.1:CDS
TGATGCAAAGTTGCTACCTT+TGG 0.408525 7:-52573554 None:intergenic
TTCCTATTTCACGTGGTTGA+TGG 0.411563 7:-52573502 None:intergenic
GATGATGCCAAGAAAGTTTG+TGG 0.440968 7:+52573178 MsaT037150.1:intron
GATATTTGAACAGCAGAAGA+TGG 0.468399 7:-52573349 None:intergenic
AGCAACTTACCATAAGAAAT+CGG 0.473971 7:-52573771 None:intergenic
GAGAAGCTTCTTCTTTCACT+TGG 0.486869 7:-52574534 None:intergenic
TTTGTGGAGAAATATGGAAA+AGG 0.487637 7:+52573194 MsaT037150.1:CDS
ATATATCTAAAGACTCCAAA+TGG 0.494837 7:+52573230 MsaT037150.1:CDS
TTAGCTGCAACTCTTTGGAA+TGG 0.508092 7:-52573431 None:intergenic
CAGAATATACCACGTATGTT+CGG 0.512344 7:+52574152 MsaT037150.1:intron
CGCATGACAACAACAAAGGA+TGG 0.548509 7:-52573730 None:intergenic
AGAAGCTTCTTCTTTCACTT+GGG 0.559960 7:-52574533 None:intergenic
CTTGCTCAAAATCTTCATGA+AGG 0.562390 7:+52572310 MsaT037150.1:CDS
CCACGTGAAATAGGAAGCAA+TGG 0.577300 7:+52573509 MsaT037150.1:CDS
GATCATCACTCTCTAGCTGA+TGG 0.591483 7:+52573326 MsaT037150.1:CDS
GTAGCAACTTTGCATCATGC+TGG 0.604292 7:+52573560 MsaT037150.1:CDS
CCATTGCTTCCTATTTCACG+TGG 0.606111 7:-52573509 None:intergenic
GTGAAAGAAGAAGCTTCTCA+AGG 0.611713 7:+52574538 MsaT037150.1:CDS
TGGTTGATGGAATTCAAAAG+AGG 0.624709 7:-52573489 None:intergenic
TGAAGTTGAGAAATTGCCAA+AGG 0.638693 7:+52573538 MsaT037150.1:CDS
TGTCATGCGTGCATCATACG+TGG 0.677837 7:+52573744 MsaT037150.1:CDS
TGCACGCATGACAACAACAA+AGG 0.693553 7:-52573734 None:intergenic
CATGCTGGCAGAGAGTCCAA+AGG 0.714363 7:+52573575 MsaT037150.1:CDS
TGTCTATTACCGAACATACG+TGG 0.717709 7:-52574161 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 52572259 52574564 52572259 ID=MsaG037150
Chr7 mRNA 52572259 52574564 52572259 ID=MsaT037150.1;Parent=MsaG037150
Chr7 exon 52572259 52572339 52572259 ID=MsaT037150.1.exon1;Parent=MsaT037150.1
Chr7 CDS 52572259 52572339 52572259 ID=cds.MsaT037150.1;Parent=MsaT037150.1
Chr7 exon 52573179 52573596 52573179 ID=MsaT037150.1.exon2;Parent=MsaT037150.1
Chr7 CDS 52573179 52573596 52573179 ID=cds.MsaT037150.1;Parent=MsaT037150.1
Chr7 exon 52573662 52573783 52573662 ID=MsaT037150.1.exon3;Parent=MsaT037150.1
Chr7 CDS 52573662 52573783 52573662 ID=cds.MsaT037150.1;Parent=MsaT037150.1
Chr7 exon 52574155 52574173 52574155 ID=MsaT037150.1.exon4;Parent=MsaT037150.1
Chr7 CDS 52574155 52574173 52574155 ID=cds.MsaT037150.1;Parent=MsaT037150.1
Chr7 exon 52574527 52574564 52574527 ID=MsaT037150.1.exon5;Parent=MsaT037150.1
Chr7 CDS 52574527 52574564 52574527 ID=cds.MsaT037150.1;Parent=MsaT037150.1
Gene Sequence

>MsaT037150.1

ATGGCTAGTGAAACTAATGAGTTGAAGCCAATCCACTTTTTCAAGATAATACTTGCTCAAAATCTTCATGAAGGAAAGCTTATGATGCCAAGAAAGTTTGTGGAGAAATATGGAAAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAAAAGTGATGGCAAGGTATGGTTTGAAAAAGGTTGGAAAGAATTTGCAGATCATCACTCTCTAGCTGATGGCCATCTTCTGCTGTTCAAATATCTAAGAAGTTCTCGTTTTCTTCTGCGCATCTTTGAAAAGAGTGCCTTAGAGATAAATTATCCATTCCAAAGAGTTGCAGCTAAAAAGGTCTCCAATGGTCAAAAGAGAAAAACTAACTCCTCTTTTGAATTCCATCAACCACGTGAAATAGGAAGCAATGGTTGTGTTGAAGTTGAGAAATTGCCAAAGGTAGCAACTTTGCATCATGCTGGCAGAGAGTCCAAAGGGAAACAAGTGCTTACTGCTAAGAGAGTAACAGCTCTTGAAAGAGCACAATCTTTCAAAACATGTAATCCATCCTTTGTTGTTGTCATGCGTGCATCATACGTGGAACACCGATTTCTTATGAATATACCACGTATGTTCGACTTTTCCCAAGTGAAAGAAGAAGCTTCTCAAGGATAA

Protein sequence

>MsaT037150.1

MASETNELKPIHFFKIILAQNLHEGKLMMPRKFVEKYGKGLSNAIYLKTPNGAKWKLNLVKSDGKVWFEKGWKEFADHHSLADGHLLLFKYLRSSRFLLRIFEKSALEINYPFQRVAAKKVSNGQKRKTNSSFEFHQPREIGSNGCVEVEKLPKVATLHHAGRESKGKQVLTAKRVTALERAQSFKTCNPSFVVVMRASYVEHRFLMNIPRMFDFSQVKEEASQG*