AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037151

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037151 MtrunA17_Chr7g0233521 79.891 184 35 2 71 253 1 183 6.13e-105 305
MsaG037151 MtrunA17_Chr7g0233541 69.347 199 44 3 71 253 1 198 1.21e-93 277
MsaG037151 MtrunA17_Chr7g0233561 64.467 197 54 2 72 253 34 229 1.64e-82 250
MsaG037151 MtrunA17_Chr7g0233571 61.538 195 56 2 69 245 23 216 3.33e-81 243
MsaG037151 MtrunA17_Chr3g0131891 63.500 200 49 4 69 253 32 222 4.57e-80 244
MsaG037151 MtrunA17_Chr7g0233531 62.121 198 44 4 71 253 1 182 1.27e-71 221
MsaG037151 MtrunA17_Chr1g0163071 34.135 208 103 4 71 244 1 208 8.90e-33 121
MsaG037151 MtrunA17_Chr1g0208771 33.333 213 104 6 72 252 13 219 6.77e-31 119
MsaG037151 MtrunA17_Chr8g0390841 34.010 197 105 3 72 243 55 251 8.79e-29 114
MsaG037151 MtrunA17_Chr3g0103191 54.217 83 38 0 72 154 26 108 1.05e-26 103
MsaG037151 MtrunA17_Chr3g0103181 34.392 189 91 4 72 244 26 197 1.49e-26 104
MsaG037151 MtrunA17_Chr3g0103151 54.217 83 38 0 72 154 26 108 4.95e-26 99.8
MsaG037151 MtrunA17_Chr5g0419201 48.810 84 43 0 71 154 31 114 5.06e-26 100
MsaG037151 MtrunA17_Chr3g0103171 50.588 85 42 0 70 154 4 88 1.13e-24 99.0
MsaG037151 MtrunA17_Chr1g0163181 33.000 200 112 6 72 253 21 216 5.44e-22 94.0
MsaG037151 MtrunA17_Chr1g0163251 32.984 191 111 6 72 253 21 203 5.35e-21 90.9
MsaG037151 MtrunA17_Chr1g0163241 39.823 113 58 2 67 171 18 128 4.18e-20 84.3
MsaG037151 MtrunA17_Chr3g0077451 29.121 182 92 6 72 249 12 160 3.03e-19 84.0
MsaG037151 MtrunA17_Chr1g0163201 30.412 194 119 4 72 253 19 208 6.54e-19 85.1
MsaG037151 MtrunA17_Chr3g0077461 38.372 86 53 0 72 157 23 108 7.36e-19 85.5
MsaG037151 MtrunA17_Chr3g0077461 41.860 86 50 0 72 157 253 338 4.88e-17 80.1
MsaG037151 MtrunA17_Chr1g0163121 39.669 121 61 3 71 191 10 118 8.73e-19 80.5
MsaG037151 MtrunA17_Chr4g0033961 32.886 149 96 2 72 216 22 170 9.89e-19 84.7
MsaG037151 MtrunA17_Chr5g0429931 36.559 93 59 0 65 157 4 96 1.14e-18 82.4
MsaG037151 MtrunA17_Chr1g0162441 42.169 83 48 0 72 154 23 105 1.94e-18 84.0
MsaG037151 MtrunA17_Chr3g0077411 38.372 86 53 0 72 157 41 126 4.69e-18 83.2
MsaG037151 MtrunA17_Chr3g0077411 39.080 87 52 1 72 157 278 364 3.02e-16 77.8
MsaG037151 MtrunA17_Chr1g0163101 38.843 121 63 3 71 191 32 141 5.26e-18 79.3
MsaG037151 MtrunA17_Chr1g0163191 44.578 83 44 1 72 154 21 101 5.89e-18 78.6
MsaG037151 MtrunA17_Chr1g0171611 29.101 189 100 4 72 253 25 186 2.28e-17 79.7
MsaG037151 MtrunA17_Chr1g0163231 30.769 182 118 5 72 253 25 198 4.61e-17 79.0
MsaG037151 MtrunA17_Chr3g0077381 37.209 86 54 0 72 157 36 121 7.25e-17 79.3
MsaG037151 MtrunA17_Chr3g0077381 37.349 83 52 0 75 157 266 348 8.71e-13 67.4
MsaG037151 MtrunA17_Chr6g0467351 34.646 127 78 2 72 197 31 153 9.57e-17 77.0
MsaG037151 MtrunA17_Chr1g0162161 31.373 153 91 4 70 220 2 142 1.53e-16 74.7
MsaG037151 MtrunA17_Chr1g0163081 37.121 132 69 5 68 199 2 119 2.99e-16 73.6
MsaG037151 MtrunA17_Chr4g0009491 36.047 86 55 0 72 157 13 98 5.08e-16 77.0
MsaG037151 MtrunA17_Chr4g0009491 38.554 83 51 0 75 157 244 326 2.28e-14 72.0
MsaG037151 MtrunA17_Chr1g0163131 36.434 129 67 4 71 199 33 146 1.25e-15 75.1
MsaG037151 MtrunA17_Chr6g0476181 33.835 133 78 5 71 199 1 127 2.41e-15 71.6
MsaG037151 MtrunA17_Chr1g0163141 40.000 95 54 2 71 165 10 101 2.45e-15 72.0
MsaG037151 MtrunA17_Chr4g0067431 39.130 92 55 1 72 162 31 122 6.06e-15 73.2
MsaG037151 MtrunA17_Chr1g0163261 28.205 195 99 5 72 245 25 199 1.32e-14 72.0
MsaG037151 MtrunA17_Chr1g0162411 39.759 83 48 1 72 154 23 103 1.03e-13 70.1
MsaG037151 MtrunA17_Chr1g0163221 37.647 85 52 1 72 155 27 111 3.28e-13 68.6
MsaG037151 MtrunA17_Chr1g0154151 30.723 166 90 3 62 227 10 150 4.08e-13 68.2
MsaG037151 MtrunA17_Chr1g0154251 38.667 75 46 0 72 146 42 116 6.76e-13 67.8
MsaG037151 MtrunA17_Chr3g0077421 37.349 83 44 2 100 182 1 75 6.84e-13 65.9
MsaG037151 MtrunA17_Chr3g0077441 37.349 83 44 2 100 182 1 75 6.84e-13 65.9
MsaG037151 MtrunA17_Chr3g0077361 29.310 116 72 3 72 178 56 170 1.37e-12 67.0
MsaG037151 MtrunA17_Chr1g0154211 38.667 75 46 0 72 146 38 112 1.70e-12 66.6
MsaG037151 MtrunA17_Chr1g0154161 25.806 186 134 2 72 253 46 231 1.94e-12 65.9
MsaG037151 MtrunA17_Chr1g0154171 26.966 178 119 5 65 232 30 206 2.15e-12 66.2
MsaG037151 MtrunA17_Chr1g0154101 30.216 139 89 4 72 204 198 334 3.93e-12 65.9
MsaG037151 MtrunA17_Chr1g0154101 31.298 131 81 2 72 196 41 168 4.16e-12 65.9
MsaG037151 MtrunA17_Chr1g0154191 37.333 75 47 0 72 146 38 112 4.44e-12 65.1
MsaG037151 MtrunA17_Chr1g0154131 25.128 195 133 4 72 253 198 392 7.33e-12 64.7
MsaG037151 MtrunA17_Chr1g0163161 32.857 140 78 5 84 220 1 127 3.20e-11 59.7
MsaG037151 MtrunA17_Chr3g0077431 29.221 154 72 6 100 249 1 121 4.28e-11 60.8
MsaG037151 MtrunA17_Chr1g0154241 29.104 134 90 2 72 200 37 170 5.06e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037151 AT1G49475.1 41.270 126 65 4 59 176 31 155 6.01e-22 90.1
MsaG037151 AT3G18990.1 48.193 83 43 0 72 154 22 104 1.15e-20 89.7
MsaG037151 AT3G18990.2 48.193 83 43 0 72 154 22 104 1.44e-20 90.1
MsaG037151 AT4G01580.1 47.826 92 46 1 70 159 42 133 2.47e-20 85.9
MsaG037151 AT3G18960.1 45.652 92 48 1 70 159 42 133 7.80e-19 82.4
MsaG037151 AT3G18960.2 45.652 92 48 1 70 159 42 133 8.77e-19 82.0
MsaG037151 AT3G18960.3 45.161 93 50 1 67 159 5 96 1.25e-18 80.9

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTAGTCTTTACCAGATTA+AGG 0.148544 7:+52596852 MsaT037151.1:intron
TGCAAAATGGAAATTAAATT+TGG 0.194062 7:+52595789 MsaT037151.1:CDS
TATTTCTCCACAAACTTTCT+TGG 0.217927 7:-52595721 None:intergenic
AGATCAACAACTAGCATATA+TGG 0.264408 7:+52591546 MsaT037151.1:CDS
AGAAAGTTTGTGGAGAAATA+TGG 0.293086 7:+52595724 MsaT037151.1:CDS
GAAAGGATAATTTATCTCTA+TGG 0.296423 7:-52595950 None:intergenic
ATGCATGGTTGTTGATTTCT+TGG 0.311840 7:+52592085 MsaT037151.1:CDS
CCTCTTCAGCTGCAACTCTT+TGG 0.320149 7:-52595972 None:intergenic
TTCCATCAACCACGTGAAAT+AGG 0.327923 7:+52596033 MsaT037151.1:CDS
AGACTCCAAATGGTGCAAAA+TGG 0.337543 7:+52595776 MsaT037151.1:CDS
ATATTTCTCACCCCTTAATC+TGG 0.364679 7:-52596864 None:intergenic
TCTAGTCTTTACCAGATTAA+GGG 0.378951 7:+52596853 MsaT037151.1:intron
TGGCAAAATATGGTTTGAAA+AGG 0.393696 7:+52595822 MsaT037151.1:CDS
TCATACGTTGAACACCGTTT+TGG 0.402302 7:+52596754 MsaT037151.1:CDS
TTCCTATTTCACGTGGTTGA+TGG 0.411563 7:-52596035 None:intergenic
TCATGGTCAACATGTAATCT+AGG 0.424926 7:-52591597 None:intergenic
GATCAACAACTAGCATATAT+GGG 0.437589 7:+52591547 MsaT037151.1:CDS
AAATATGGTTTGAAAAGGGT+TGG 0.440218 7:+52595827 MsaT037151.1:CDS
GATGATGCCAAGAAAGTTTG+TGG 0.440968 7:+52595714 MsaT037151.1:intron
GTTGCAGCTGAAGAGGTATC+TGG 0.450766 7:+52595979 MsaT037151.1:CDS
TCTGGTGAGGAATACTCAAA+TGG 0.459266 7:+52591440 MsaT037151.1:CDS
GAAGTTTGCTTTCTGTAAGA+AGG 0.461761 7:+52593860 MsaT037151.1:CDS
TTTGTGGAGAAATATGGAAA+AGG 0.487637 7:+52595730 MsaT037151.1:CDS
TGAAGTTGAGAAATTGCAAA+AGG 0.487851 7:+52596071 MsaT037151.1:CDS
GGCAAAATATGGTTTGAAAA+GGG 0.493730 7:+52595823 MsaT037151.1:CDS
CCAAAGAGTTGCAGCTGAAG+AGG 0.494567 7:+52595972 MsaT037151.1:CDS
ATATATCTAAAGACTCCAAA+TGG 0.494837 7:+52595766 MsaT037151.1:CDS
CTGGTGAGGAATACTCAAAT+GGG 0.516417 7:+52591441 MsaT037151.1:CDS
CAACTAGCATATATGGGGAT+AGG 0.540324 7:+52591553 MsaT037151.1:CDS
CATATCTGAAAAGCACAAGA+TGG 0.540339 7:-52595885 None:intergenic
CGCATGGCAACAACAAAGGA+TGG 0.550805 7:-52596727 None:intergenic
GAGTATCATTCTCTAGCTCA+TGG 0.575939 7:+52595862 MsaT037151.1:CDS
TCAGCTGCAACTCTTTGGAA+AGG 0.577291 7:-52595967 None:intergenic
CCACGTGAAATAGGAAGCAA+TGG 0.577300 7:+52596042 MsaT037151.1:CDS
TAGCATATATGGGGATAGGA+AGG 0.578288 7:+52591557 MsaT037151.1:CDS
TGCAACTTACCAGACCAAAA+CGG 0.594101 7:-52596768 None:intergenic
ATCTTCTTCTTCACACCACG+AGG 0.600231 7:-52593837 None:intergenic
GTAGCAACTTTGCATCATGC+TGG 0.604292 7:+52596093 MsaT037151.1:CDS
CCATTGCTTCCTATTTCACG+TGG 0.606111 7:-52596042 None:intergenic
TGGTTGATGGAATTCAAAAG+AGG 0.624709 7:-52596022 None:intergenic
TTCAACGTATGATGCACGCA+TGG 0.638803 7:-52596743 None:intergenic
TGCACGCATGGCAACAACAA+AGG 0.639444 7:-52596731 None:intergenic
ATCAACAACTAGCATATATG+GGG 0.644443 7:+52591548 MsaT037151.1:CDS
CATGCTGGCAGAGAGTCCAA+AGG 0.714363 7:+52596108 MsaT037151.1:CDS
TAGATTACATGTTGACCATG+AGG 0.737910 7:+52591599 MsaT037151.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 52591437 52596872 52591437 ID=MsaG037151
Chr7 mRNA 52591437 52596872 52591437 ID=MsaT037151.1;Parent=MsaG037151
Chr7 exon 52591437 52591462 52591437 ID=MsaT037151.1.exon1;Parent=MsaT037151.1
Chr7 CDS 52591437 52591462 52591437 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Chr7 exon 52591530 52591620 52591530 ID=MsaT037151.1.exon2;Parent=MsaT037151.1
Chr7 CDS 52591530 52591620 52591530 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Chr7 exon 52592071 52592114 52592071 ID=MsaT037151.1.exon3;Parent=MsaT037151.1
Chr7 CDS 52592071 52592114 52592071 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Chr7 exon 52593833 52593881 52593833 ID=MsaT037151.1.exon4;Parent=MsaT037151.1
Chr7 CDS 52593833 52593881 52593833 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Chr7 exon 52595715 52596129 52595715 ID=MsaT037151.1.exon5;Parent=MsaT037151.1
Chr7 CDS 52595715 52596129 52595715 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Chr7 exon 52596656 52596780 52596656 ID=MsaT037151.1.exon6;Parent=MsaT037151.1
Chr7 CDS 52596656 52596780 52596656 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Chr7 exon 52596858 52596872 52596858 ID=MsaT037151.1.exon7;Parent=MsaT037151.1
Chr7 CDS 52596858 52596872 52596858 ID=cds.MsaT037151.1;Parent=MsaT037151.1
Gene Sequence

>MsaT037151.1

ATGTCTGGTGAGGAATACTCAAATGGTGACGAGCTTTTGATCAGATCAACAACTAGCATATATGGGGATAGGAAGGCTCTAAAAACACTGATGCCTAGATTACATGTTGACCATGAGGGGGATTCACTGATATGCATGGTTGTTGATTTCTTGGAGAACAATGGACCTCGTGGTGTGAAGAAGAAGATGAAGTTTGCTTTCTGTAAGAAGATGATGCCAAGAAAGTTTGTGGAGAAATATGGAAAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAAAAGTGATGGCAAAATATGGTTTGAAAAGGGTTGGAAAGAATTTGCAGAGTATCATTCTCTAGCTCATGGCCATCTTGTGCTTTTCAGATATGAAAAAACTTCTCTTTTTGAGGTACATATCTTTGATAAGAGTGCCATAGAGATAAATTATCCTTTCCAAAGAGTTGCAGCTGAAGAGGTATCTGGTGAAAAGAGAAAAGCTAACTCCTCTTTTGAATTCCATCAACCACGTGAAATAGGAAGCAATGGTTGTGTTGAAGTTGAGAAATTGCAAAAGGTAGCAACTTTGCATCATGCTGGCAGAGAGTCCAAAGGAAAACAAGTGATTCCTACTGCTGAGAAAATTACAACTGTTGAAAGAGCACAATCTTTCAAAACAAGTAATCCATCCTTTGTTGTTGCCATGCGTGCATCATACGTTGAACACCGTTTTGGTCTGTCTTTACCAGATTAA
Protein sequence

>MsaT037151.1

MSGEEYSNGDELLIRSTTSIYGDRKALKTLMPRLHVDHEGDSLICMVVDFLENNGPRGVKKKMKFAFCKKMMPRKFVEKYGKGLSNAIYLKTPNGAKWKLNLVKSDGKIWFEKGWKEFAEYHSLAHGHLVLFRYEKTSLFEVHIFDKSAIEINYPFQRVAAEEVSGEKRKANSSFEFHQPREIGSNGCVEVEKLQKVATLHHAGRESKGKQVIPTAEKITTVERAQSFKTSNPSFVVAMRASYVEHRFGLSLPD*