AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037152

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037152 MtrunA17_Chr7g0233521 81.467 259 47 1 78 336 1 258 6.83e-154 443
MsaG037152 MtrunA17_Chr7g0233521 52.055 292 66 8 365 598 1 276 4.01e-78 249
MsaG037152 MtrunA17_Chr7g0233541 71.273 275 63 3 78 336 1 275 8.84e-137 400
MsaG037152 MtrunA17_Chr7g0233541 52.525 297 74 7 365 598 1 293 3.34e-84 265
MsaG037152 MtrunA17_Chr7g0233561 65.461 304 87 4 50 336 5 307 1.63e-129 383
MsaG037152 MtrunA17_Chr7g0233561 44.512 328 107 10 339 598 5 325 6.51e-70 229
MsaG037152 MtrunA17_Chr3g0131891 63.696 303 86 5 48 335 4 297 1.63e-125 373
MsaG037152 MtrunA17_Chr3g0131891 51.562 320 90 9 337 598 4 316 1.77e-86 272
MsaG037152 MtrunA17_Chr7g0233531 64.103 273 69 4 78 336 1 258 1.14e-112 338
MsaG037152 MtrunA17_Chr7g0233531 58.246 285 59 4 365 598 1 276 1.85e-103 314
MsaG037152 MtrunA17_Chr7g0233571 63.551 214 61 1 55 251 2 215 4.33e-90 278
MsaG037152 MtrunA17_Chr7g0233571 60.784 204 67 7 340 534 1 200 1.89e-65 214
MsaG037152 MtrunA17_Chr8g0390841 37.855 317 159 9 45 335 21 325 3.56e-51 184
MsaG037152 MtrunA17_Chr8g0390841 38.000 150 89 2 348 496 37 183 1.48e-27 117
MsaG037152 MtrunA17_Chr8g0390841 41.111 90 43 2 499 588 255 334 1.34e-11 67.4
MsaG037152 MtrunA17_Chr3g0077411 25.812 554 319 15 62 584 24 516 3.75e-49 179
MsaG037152 MtrunA17_Chr3g0077411 26.948 308 177 11 30 328 236 504 1.93e-22 101
MsaG037152 MtrunA17_Chr3g0077411 28.395 243 147 8 338 572 13 236 3.01e-21 97.8
MsaG037152 MtrunA17_Chr1g0208771 35.739 291 134 10 79 335 13 284 4.12e-46 169
MsaG037152 MtrunA17_Chr1g0208771 31.757 148 97 1 366 509 13 160 3.54e-21 97.1
MsaG037152 MtrunA17_Chr1g0208771 37.931 87 44 2 502 588 217 293 1.89e-11 66.6
MsaG037152 MtrunA17_Chr1g0163071 35.052 291 144 6 78 334 1 280 1.93e-45 163
MsaG037152 MtrunA17_Chr1g0163071 34.641 153 85 3 365 503 1 152 1.01e-23 102
MsaG037152 MtrunA17_Chr1g0163071 43.956 91 40 3 502 592 215 294 3.73e-13 70.9
MsaG037152 MtrunA17_Chr3g0077461 27.432 514 280 16 79 576 23 459 9.22e-45 166
MsaG037152 MtrunA17_Chr3g0077461 28.477 302 156 9 30 328 211 455 1.48e-21 98.2
MsaG037152 MtrunA17_Chr3g0103181 35.216 301 149 11 53 335 2 274 3.71e-39 145
MsaG037152 MtrunA17_Chr3g0103181 32.857 280 137 11 347 584 9 279 6.70e-32 125
MsaG037152 MtrunA17_Chr4g0009491 27.692 390 217 9 77 466 11 335 8.60e-35 136
MsaG037152 MtrunA17_Chr4g0009491 28.636 220 137 7 364 578 11 215 1.66e-19 91.7
MsaG037152 MtrunA17_Chr4g0009491 35.965 114 71 1 49 162 213 324 9.39e-15 77.0
MsaG037152 MtrunA17_Chr1g0163181 28.066 424 237 13 72 438 14 426 9.55e-35 136
MsaG037152 MtrunA17_Chr1g0163181 30.263 304 139 10 340 583 1 291 4.07e-24 105
MsaG037152 MtrunA17_Chr3g0077361 22.604 553 315 15 62 588 39 504 2.28e-32 131
MsaG037152 MtrunA17_Chr3g0077361 26.754 228 154 6 347 572 37 253 7.70e-21 96.3
MsaG037152 MtrunA17_Chr1g0154111 26.027 438 236 10 61 434 24 437 1.80e-31 129
MsaG037152 MtrunA17_Chr1g0163201 29.146 398 241 14 79 449 19 402 2.39e-30 123
MsaG037152 MtrunA17_Chr3g0103191 33.659 205 119 4 56 251 5 201 2.84e-30 119
MsaG037152 MtrunA17_Chr3g0103191 50.495 101 48 1 348 448 10 108 1.11e-26 109
MsaG037152 MtrunA17_Chr1g0163231 29.568 301 170 8 60 343 6 281 4.63e-30 120
MsaG037152 MtrunA17_Chr1g0163231 31.429 280 135 7 350 583 9 277 6.48e-27 111
MsaG037152 MtrunA17_Chr3g0103151 52.941 102 46 1 60 161 9 108 2.46e-29 114
MsaG037152 MtrunA17_Chr3g0103151 50.495 101 48 1 348 448 10 108 3.65e-26 105
MsaG037152 MtrunA17_Chr1g0171611 33.840 263 139 8 350 583 9 265 2.97e-29 117
MsaG037152 MtrunA17_Chr1g0171611 29.930 284 179 5 60 343 6 269 1.00e-28 116
MsaG037152 MtrunA17_Chr5g0419201 38.095 147 84 2 350 489 16 162 1.16e-28 113
MsaG037152 MtrunA17_Chr5g0419201 43.243 111 60 1 63 170 16 126 7.99e-28 110
MsaG037152 MtrunA17_Chr4g0067431 30.847 295 166 11 61 327 13 297 3.82e-28 115
MsaG037152 MtrunA17_Chr4g0067431 28.105 306 144 9 348 584 13 311 2.83e-21 95.1
MsaG037152 MtrunA17_Chr1g0163221 27.250 400 229 12 55 438 3 356 6.03e-28 115
MsaG037152 MtrunA17_Chr1g0163221 28.667 300 149 10 340 584 1 290 3.29e-23 101
MsaG037152 MtrunA17_Chr1g0163251 31.034 290 173 10 63 342 9 281 7.16e-28 115
MsaG037152 MtrunA17_Chr1g0163251 50.000 90 43 1 359 448 14 101 1.58e-18 88.2
MsaG037152 MtrunA17_Chr1g0163261 29.097 299 162 9 60 335 6 277 9.26e-27 110
MsaG037152 MtrunA17_Chr1g0163261 29.930 284 156 6 350 592 9 290 3.91e-22 97.4
MsaG037152 MtrunA17_Chr3g0103171 30.182 275 154 10 79 335 6 260 2.87e-26 108
MsaG037152 MtrunA17_Chr3g0103171 49.398 83 42 0 366 448 6 88 8.71e-20 89.7
MsaG037152 MtrunA17_Chr3g0077451 28.452 239 146 6 364 588 10 237 4.27e-26 107
MsaG037152 MtrunA17_Chr3g0077451 25.843 267 140 6 77 334 10 227 8.14e-22 95.1
MsaG037152 MtrunA17_Chr5g0429931 28.755 233 152 6 366 585 11 242 4.64e-25 104
MsaG037152 MtrunA17_Chr5g0429931 26.154 260 145 6 79 330 11 231 1.16e-18 85.9
MsaG037152 MtrunA17_Chr1g0154131 29.861 288 170 10 61 328 182 457 4.58e-24 105
MsaG037152 MtrunA17_Chr6g0467351 47.573 103 53 1 61 162 13 115 1.97e-23 99.4
MsaG037152 MtrunA17_Chr6g0467351 35.862 145 87 3 348 486 13 157 6.23e-18 83.6
MsaG037152 MtrunA17_Chr1g0154171 25.153 326 192 6 56 333 16 337 2.28e-23 102
MsaG037152 MtrunA17_Chr1g0154161 26.207 290 181 7 61 333 28 301 5.19e-23 100
MsaG037152 MtrunA17_Chr1g0154161 25.581 301 158 11 348 593 28 317 2.59e-17 83.6
MsaG037152 MtrunA17_Chr1g0163131 27.187 320 176 9 56 341 11 307 1.19e-20 93.2
MsaG037152 MtrunA17_Chr1g0163131 42.574 101 55 2 348 448 16 113 6.96e-15 75.9
MsaG037152 MtrunA17_Chr1g0154191 24.484 339 190 9 56 333 15 348 6.02e-20 92.0
MsaG037152 MtrunA17_Chr1g0163101 35.862 145 73 4 59 199 13 141 1.35e-19 87.0
MsaG037152 MtrunA17_Chr1g0163101 43.810 105 56 2 344 448 11 112 4.63e-18 82.4
MsaG037152 MtrunA17_Chr1g0162441 42.574 101 58 0 63 163 7 107 1.78e-19 91.3
MsaG037152 MtrunA17_Chr1g0162441 41.414 99 58 0 350 448 7 105 2.51e-18 87.8
MsaG037152 MtrunA17_Chr1g0162441 26.623 154 106 3 215 367 272 419 3.54e-11 65.9
MsaG037152 MtrunA17_Chr4g0033961 32.075 159 105 2 63 218 6 164 6.58e-19 89.7
MsaG037152 MtrunA17_Chr4g0033961 38.889 108 64 1 347 452 3 110 2.32e-17 84.7
MsaG037152 MtrunA17_Chr4g0033961 31.034 116 74 2 215 330 268 377 3.96e-11 65.5
MsaG037152 MtrunA17_Chr1g0163241 37.931 145 77 4 366 499 23 165 1.53e-18 83.6
MsaG037152 MtrunA17_Chr1g0163241 48.193 83 41 1 79 161 23 103 4.45e-17 79.3
MsaG037152 MtrunA17_Chr1g0154071 24.179 335 166 7 64 328 157 473 1.84e-18 89.0
MsaG037152 MtrunA17_Chr3g0077431 28.708 209 124 6 394 588 1 198 2.07e-18 84.0
MsaG037152 MtrunA17_Chr3g0077431 25.424 236 118 6 107 333 1 187 7.28e-14 70.9
MsaG037152 MtrunA17_Chr1g0163191 44.444 90 48 1 72 161 14 101 1.45e-17 80.5
MsaG037152 MtrunA17_Chr1g0163191 45.556 90 47 1 359 448 14 101 1.70e-17 80.1
MsaG037152 MtrunA17_Chr3g0077381 27.727 220 127 6 364 583 34 221 3.01e-17 84.7
MsaG037152 MtrunA17_Chr3g0077381 30.994 171 97 2 311 466 193 357 6.06e-16 80.5
MsaG037152 MtrunA17_Chr3g0077381 32.759 116 75 1 77 192 34 146 1.13e-15 79.7
MsaG037152 MtrunA17_Chr3g0077381 34.815 135 79 3 34 162 215 346 3.11e-13 72.0
MsaG037152 MtrunA17_Chr3g0103201 29.825 228 137 8 118 335 1 215 3.63e-17 80.9
MsaG037152 MtrunA17_Chr1g0162411 40.404 99 57 1 350 448 7 103 1.01e-16 83.2
MsaG037152 MtrunA17_Chr1g0162411 39.604 101 59 1 63 163 7 105 3.85e-15 78.2
MsaG037152 MtrunA17_Chr1g0154151 23.824 319 187 7 66 343 14 317 1.06e-16 81.6
MsaG037152 MtrunA17_Chr1g0163061 40.566 106 56 2 61 162 13 115 1.87e-16 80.1
MsaG037152 MtrunA17_Chr1g0163061 40.000 105 62 1 348 451 13 117 7.61e-15 75.1
MsaG037152 MtrunA17_Chr1g0163121 39.024 123 62 3 77 199 9 118 2.19e-16 76.6
MsaG037152 MtrunA17_Chr1g0163121 50.000 92 43 2 361 452 6 94 3.80e-16 75.9
MsaG037152 MtrunA17_Chr1g0154101 22.484 467 285 17 61 469 182 629 7.79e-16 81.3
MsaG037152 MtrunA17_Chr1g0154101 32.000 150 92 2 61 204 23 168 6.17e-13 72.0
MsaG037152 MtrunA17_Chr1g0154101 34.783 92 60 0 348 439 23 114 1.07e-11 68.2
MsaG037152 MtrunA17_Chr1g0154101 28.571 126 85 2 348 470 182 305 8.10e-11 65.1
MsaG037152 MtrunA17_Chr1g0154251 37.634 93 57 1 61 153 25 116 2.56e-14 75.9
MsaG037152 MtrunA17_Chr1g0154251 33.333 93 61 1 348 440 25 116 2.78e-11 66.2
MsaG037152 MtrunA17_Chr1g0163141 35.593 118 73 2 77 194 9 123 1.01e-13 70.1
MsaG037152 MtrunA17_Chr1g0163141 43.956 91 48 2 364 454 9 96 1.87e-13 69.3
MsaG037152 MtrunA17_Chr1g0154211 35.714 98 62 1 56 153 16 112 1.15e-13 73.2
MsaG037152 MtrunA17_Chr1g0154211 34.409 93 60 1 348 440 21 112 6.59e-12 67.8
MsaG037152 MtrunA17_Chr3g0077421 26.316 228 123 8 107 331 1 186 3.92e-13 68.6
MsaG037152 MtrunA17_Chr3g0077421 28.218 202 129 7 394 585 1 196 1.04e-12 67.4
MsaG037152 MtrunA17_Chr3g0077441 26.316 228 123 8 107 331 1 186 3.92e-13 68.6
MsaG037152 MtrunA17_Chr3g0077441 28.218 202 129 7 394 585 1 196 1.04e-12 67.4
MsaG037152 MtrunA17_Chr1g0154241 28.643 199 110 6 61 234 20 211 8.98e-13 68.9
MsaG037152 MtrunA17_Chr6g0476181 45.349 86 44 2 78 163 1 83 3.23e-12 65.1
MsaG037152 MtrunA17_Chr6g0476181 46.429 84 42 2 365 448 1 81 1.08e-11 63.5
MsaG037152 MtrunA17_Chr1g0162161 35.200 125 62 4 78 199 3 111 1.43e-11 63.2
MsaG037152 MtrunA17_Chr1g0162161 39.362 94 54 2 365 458 3 93 7.02e-11 61.2
MsaG037152 MtrunA17_Chr1g0163081 34.615 130 70 5 78 207 5 119 2.44e-11 62.0
MsaG037152 MtrunA17_Chr1g0163151 27.074 229 136 10 229 438 2 218 3.47e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037152 AT4G01580.1 41.129 124 70 2 42 162 6 129 7.22e-23 97.1
MsaG037152 AT4G01580.1 40.385 104 62 0 350 453 30 133 1.74e-18 84.3
MsaG037152 AT1G49475.1 47.000 100 52 1 63 162 34 132 1.42e-22 96.3
MsaG037152 AT1G49475.1 41.584 101 58 1 350 450 34 133 2.53e-17 80.9
MsaG037152 AT3G18990.1 45.455 99 54 0 63 161 6 104 1.55e-22 99.8
MsaG037152 AT3G18990.1 42.424 99 57 0 350 448 6 104 5.33e-19 89.4
MsaG037152 AT3G18990.2 45.455 99 54 0 63 161 6 104 2.67e-22 99.8
MsaG037152 AT3G18990.2 42.424 99 57 0 350 448 6 104 9.92e-19 89.0
MsaG037152 AT3G18960.2 44.000 100 56 0 63 162 30 129 2.79e-21 92.4
MsaG037152 AT3G18960.2 39.423 104 63 0 350 453 30 133 3.94e-17 80.5
MsaG037152 AT3G18960.1 44.000 100 56 0 63 162 30 129 3.47e-21 92.8
MsaG037152 AT3G18960.1 39.423 104 63 0 350 453 30 133 4.53e-17 80.9
MsaG037152 AT4G33280.1 28.477 302 172 14 58 330 20 306 3.01e-19 89.7
MsaG037152 AT3G18960.3 47.619 84 44 0 79 162 9 92 1.43e-17 81.3
MsaG037152 AT3G18960.3 44.318 88 49 0 366 453 9 96 6.57e-16 76.3
MsaG037152 AT3G17010.1 24.490 294 171 10 57 328 15 279 8.07e-12 67.0
MsaG037152 AT4G31650.1 25.000 316 185 13 304 578 66 370 1.30e-11 67.8
MsaG037152 AT3G53310.1 33.333 102 66 2 53 154 2 101 2.90e-11 65.1

Find 95 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATGGAAATTAAATT+TGG 0.194062 7:+52619780 MsaT037152.1:CDS
TATTTCTCCACAAACTTTCT+TGG 0.232393 7:-52619712 None:intergenic
CTTCATCAACCGCATGAAAT+TGG 0.235767 7:+52606786 MsaT037152.1:CDS
TGCAAAGTGGAACTTAAATT+TGG 0.244297 7:+52612601 MsaT037152.1:CDS
TTCCATCAACCATGTGAAAT+TGG 0.259052 7:+52612848 MsaT037152.1:CDS
TAAAGGGTAATTTATCTCTA+AGG 0.280934 7:-52619941 None:intergenic
GGAAGGATAATTTATCTCTA+AGG 0.288679 7:-52612762 None:intergenic
GAGAGGAGATGTTTATTTGC+AGG 0.289134 7:+52613818 MsaT037152.1:CDS
TAAATAGTGATGGCAAAATA+TGG 0.289847 7:+52612624 MsaT037152.1:CDS
CTAATCAGGATGACCCTTAC+AGG 0.321649 7:+52613873 MsaT037152.1:CDS
AGACTCCAAATGGTGCAAAA+TGG 0.337543 7:+52619767 MsaT037152.1:CDS
GAGTTTCAGCTAAAGCTAAC+AGG 0.351055 7:+52619968 MsaT037152.1:CDS
TCTAGCGCATGGCCATCTTC+TGG 0.364197 7:+52619864 MsaT037152.1:CDS
TGGTAATAGACACTTTGATT+TGG 0.367753 7:+52613788 MsaT037152.1:CDS
TAGCTGAAACTCTTTGTAAA+GGG 0.374498 7:-52619957 None:intergenic
CTTCATCAACCACGTGAAAT+CGG 0.381082 7:+52620072 MsaT037152.1:CDS
ATCCTGATTAGGTACTTTGC+AGG 0.384916 7:-52613861 None:intergenic
CGCACTCAAAATCTTCATAA+AGG 0.387601 7:+52618639 MsaT037152.1:CDS
AAGTTCCACTTTGCACCATT+TGG 0.391571 7:-52612593 None:intergenic
TGGCAAAATATGGTTTGAAA+AGG 0.393696 7:+52612634 MsaT037152.1:CDS
TGGTATGAGACACTTCGATA+TGG 0.395031 7:+52631861 MsaT037152.1:CDS
TTCCAATTTCACATGGTTGA+TGG 0.398771 7:-52612850 None:intergenic
CAGGTTATACCGTCTATGTT+TGG 0.399062 7:+52631841 MsaT037152.1:intron
AGGTGTTAAATGAAGGAGTT+TGG 0.403488 7:+52613838 MsaT037152.1:CDS
CAGAATATACCACGTATGTT+TGG 0.412403 7:+52613768 MsaT037152.1:intron
TTAGCTGAAACTCTTTGTAA+AGG 0.420980 7:-52619958 None:intergenic
CTGTAAGGGTCATCCTGATT+AGG 0.427523 7:-52613872 None:intergenic
GTCTAACATGTGGTCCTGTA+AGG 0.429846 7:-52613887 None:intergenic
TGTTAACTAGTGGAAGAGTT+TGG 0.436159 7:+52631914 MsaT037152.1:CDS
AAATATGGTTTGAAAAGGGT+TGG 0.440218 7:+52612639 MsaT037152.1:CDS
AAATATGCTTTGAAAAGGGT+TGG 0.440218 7:+52619818 MsaT037152.1:CDS
CTCACTCATGGCTAGTGAAA+TGG 0.441955 7:+52618587 MsaT037152.1:CDS
GGCCTGCAAAGTACCTAATC+AGG 0.445165 7:+52613859 MsaT037152.1:CDS
GATGATGCCAAGAAAGTTTG+TGG 0.445895 7:+52619705 MsaT037152.1:intron
CTTCGATATGGAAAAGAAGA+AGG 0.460858 7:+52631873 MsaT037152.1:CDS
GATATTTGAAAACCAGAAGA+TGG 0.469802 7:-52619876 None:intergenic
GGACCACATGTTAGACTCTC+AGG 0.469882 7:+52613894 MsaT037152.1:CDS
TATTTGCAGGTGTTAAATGA+AGG 0.472581 7:+52613831 MsaT037152.1:CDS
AGAAAGTTTGTGGAGAAATA+CGG 0.478652 7:+52619715 MsaT037152.1:CDS
CATATCTGAAAAGCAGAAGA+TGG 0.486489 7:-52612697 None:intergenic
AAGGACAATAACTTAAAAGT+TGG 0.486873 7:+52632012 MsaT037152.1:CDS
TGAAGTTGAGAAATTGCAAA+AGG 0.487851 7:+52612886 MsaT037152.1:CDS
GGCAAAATATGGTTTGAAAA+GGG 0.493730 7:+52612635 MsaT037152.1:CDS
ATATATCTAAAGACTCCAAA+TGG 0.494837 7:+52612578 MsaT037152.1:CDS
ATATATCTAAAGACTCCAAA+TGG 0.494837 7:+52619757 MsaT037152.1:CDS
TACAGTTGGAAAATTTCAAA+AGG 0.500148 7:+52606824 MsaT037152.1:CDS
ATAAAAGAAGAAGATATTCA+AGG 0.505457 7:+52632234 MsaT037152.1:CDS
AGGTTTATTTCCTTGACCAT+TGG 0.507461 7:-52606709 None:intergenic
TCTAAAGATGTGAACAATGA+AGG 0.509907 7:-52614000 None:intergenic
CAAGGAAATAAACCTCCAAA+TGG 0.511352 7:+52606717 MsaT037152.1:CDS
CAAGGAAATAAACTTCCAAA+TGG 0.511352 7:+52620003 MsaT037152.1:CDS
GAGGACAATAACTTGAAAGT+TGG 0.514989 7:+52613936 MsaT037152.1:CDS
TTAAATTTGGTAAATAGTGA+TGG 0.516606 7:+52612614 MsaT037152.1:CDS
AAAGCTAACAGGGTCTCAAA+TGG 0.527649 7:+52619979 MsaT037152.1:CDS
ACATTTAAATTTGAACTCAC+AGG 0.533222 7:+52631970 MsaT037152.1:CDS
TGCGTATGATCTTGAGAAAG+TGG 0.539949 7:-52618620 None:intergenic
AATTTGAACTCACAGGTGGA+TGG 0.546820 7:+52631977 MsaT037152.1:CDS
TGCAACTTACCAGAAGAAAA+CGG 0.548608 7:-52613211 None:intergenic
TTTAAATTTGAACTCACAGG+TGG 0.549372 7:+52631973 MsaT037152.1:CDS
TTAGCTGCAAAACTTTGGGA+AGG 0.551472 7:-52612779 None:intergenic
CCATTGCTTCCAATTTCACA+TGG 0.553545 7:-52612857 None:intergenic
ATGTTAGACTCTCAGGTGGA+TGG 0.557800 7:+52613901 MsaT037152.1:CDS
CGCATGACAACAAGAAAGGA+TGG 0.561092 7:-52613170 None:intergenic
ATGCGGTTGATGAAGTTCAA+AGG 0.562344 7:-52606778 None:intergenic
ACGTGGTTGATGAAGTTCAA+AGG 0.562344 7:-52620064 None:intergenic
CTTGCTCAAAATCTTCATGA+AGG 0.562390 7:+52611683 MsaT037152.1:CDS
CCATGTGAAATTGGAAGCAA+TGG 0.565270 7:+52612857 MsaT037152.1:CDS
GTGGAAGAGTTTGGTCTGCA+AGG 0.568632 7:+52631923 MsaT037152.1:CDS
GTAGCAACTTTAAATCATGC+TGG 0.574431 7:+52612908 MsaT037152.1:CDS
GAGCATCACTCTCTAGCTCA+TGG 0.577825 7:+52612674 MsaT037152.1:CDS
TTTGTGGAGAAATATGGAGA+AGG 0.578281 7:+52612542 MsaT037152.1:CDS
TTTGTGGAGAAATACGGAGA+AGG 0.580720 7:+52619721 MsaT037152.1:CDS
CACTGTTCACATCTTTAGAG+AGG 0.585792 7:+52632080 MsaT037152.1:CDS
GAGCATCACTCTCTAGCGCA+TGG 0.589556 7:+52619853 MsaT037152.1:CDS
TTTGATTTGGATCAGAAGAG+AGG 0.591832 7:+52613801 MsaT037152.1:CDS
TTCGATATGGAAAAGAAGAA+GGG 0.592083 7:+52631874 MsaT037152.1:CDS
AACAGGGTCTCAAATGGTCA+AGG 0.596416 7:+52619985 MsaT037152.1:CDS
TCTAACATGTGGTCCTGTAA+GGG 0.599023 7:-52613886 None:intergenic
TAGACTCTCAGGTGGATGGA+AGG 0.601234 7:+52613905 MsaT037152.1:CDS
TGCACGCATGACAACAAGAA+AGG 0.606198 7:-52613174 None:intergenic
CTACTGCTTCCGATTTCACG+TGG 0.606234 7:-52620081 None:intergenic
TAGCAAGCAGTACTCACTCA+TGG 0.607806 7:+52611613 MsaT037152.1:intron
AATAGGGTCTCCAATGGTCA+AGG 0.609037 7:+52606699 None:intergenic
CTACTGCTTCCAATTTCATG+CGG 0.623346 7:-52606795 None:intergenic
TGGTTGATGGAATTCAAAAG+AGG 0.626481 7:-52612837 None:intergenic
AGACTCCAAATGGTGCAAAG+TGG 0.626570 7:+52612588 MsaT037152.1:CDS
TAGCACGGAGTACTCACTCA+TGG 0.632921 7:+52618575 MsaT037152.1:intron
AGTTTCAGCTAAAGCTAACA+GGG 0.634428 7:+52619969 MsaT037152.1:CDS
TGGATGGAAAGCATTTGCAA+AGG 0.665042 7:+52631993 MsaT037152.1:CDS
TGGATGGAAGGCATTTGCAG+AGG 0.673309 7:+52613917 MsaT037152.1:CDS
TGTCTCATACCAAACATAGA+CGG 0.687526 7:-52631850 None:intergenic
CCACATGTTAGACTCTCAGG+TGG 0.694586 7:+52613897 MsaT037152.1:CDS
CCACCTGAGAGTCTAACATG+TGG 0.731917 7:-52613897 None:intergenic
TGTCTATTACCAAACATACG+TGG 0.737084 7:-52613777 None:intergenic
CATGCTGGCAGAGAGTCCAG+AGG 0.757168 7:+52612923 MsaT037152.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 52606712 52632260 52606712 ID=MsaG037152
Chr7 mRNA 52606712 52632260 52606712 ID=MsaT037152.1;Parent=MsaG037152
Chr7 exon 52606712 52606845 52606712 ID=MsaT037152.1.exon1;Parent=MsaT037152.1
Chr7 CDS 52606712 52606845 52606712 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52611616 52611712 52611616 ID=MsaT037152.1.exon2;Parent=MsaT037152.1
Chr7 CDS 52611616 52611712 52611616 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52612527 52612944 52612527 ID=MsaT037152.1.exon3;Parent=MsaT037152.1
Chr7 CDS 52612527 52612944 52612527 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52613102 52613223 52613102 ID=MsaT037152.1.exon4;Parent=MsaT037152.1
Chr7 CDS 52613102 52613223 52613102 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52613771 52614007 52613771 ID=MsaT037152.1.exon5;Parent=MsaT037152.1
Chr7 CDS 52613771 52614007 52613771 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52618585 52618668 52618585 ID=MsaT037152.1.exon6;Parent=MsaT037152.1
Chr7 CDS 52618585 52618668 52618585 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52619706 52620113 52619706 ID=MsaT037152.1.exon7;Parent=MsaT037152.1
Chr7 CDS 52619706 52620113 52619706 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52631844 52632138 52631844 ID=MsaT037152.1.exon8;Parent=MsaT037152.1
Chr7 CDS 52631844 52632138 52631844 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Chr7 exon 52632223 52632260 52632223 ID=MsaT037152.1.exon9;Parent=MsaT037152.1
Chr7 CDS 52632223 52632260 52632223 ID=cds.MsaT037152.1;Parent=MsaT037152.1
Gene Sequence

>MsaT037152.1

ATGGTCAAGGAAATAAACCTCCAAATGGTGAAAATTGTAGAGCATCTCAAAAGAGAAAAGATAACTCCTTTGAACTTCATCAACCGCATGAAATTGGAAGCAGTAGTTGTTTTACAGTTGGAAAATTTCAAAAGCAAGCAGTACTCACTCATGGCTAGTGAAACTAATGAGTTGAAGCCAATCCACTTTTTCAAGATAATACTTGCTCAAAATCTTCATGAAGGAAAGCTTATGATACCAAAAAAGTTTGTGGAGAAATATGGAGAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAATGGTGCAAAGTGGAACTTAAATTTGGTAAATAGTGATGGCAAAATATGGTTTGAAAAGGGTTGGATAGAATTTGCAGAGCATCACTCTCTAGCTCATGGCCATCTTCTGCTTTTCAGATATGAAAAAACTTCACTTTTTGAGGTACATATCTTTGATAAGAGTGCCTTAGAGATAAATTATCCTTCCCAAAGTTTTGCAGCTAAAAAGGTCTCCAGTGGTCAAAAGATAAAAGCTAACTCCTCTTTTGAATTCCATCAACCATGTGAAATTGGAAGCAATGGTTGTGTTGAAGTTGAGAAATTGCAAAAGGTAGCAACTTTAAATCATGCTGGCAGAGAGTCCAGAGGAAAACAAGTGATTACTGCTAAGAAAGTTACAACTCTTGAAAGAGCACAATCTTTCAAAACATGCAATCCATCCTTTCTTGTTGTCATGCGTGCATCATACGTTAAACACCGTTTTCTTCTGAATATACCACGTATGTTTGGTAATAGACACTTTGATTTGGATCAGAAGAGAGGAGATGTTTATTTGCAGGTGTTAAATGAAGGAGTTTGGCCTGCAAAGTACCTAATCAGGATGACCCTTACAGGACCACATGTTAGACTCTCAGGTGGATGGAAGGCATTTGCAGAGGACAATAACTTGAAAGTTGGCGATGTTTGCAAATTTGAGCTCATCTCTAGCACTATACTTACCTTCATTTACTCACTCATGGCTAGTGAAATGGTGAGATCAGTCCACTTTCTCAAGATCATACGCACTCAAAATCTTCATAAAGGAAAGCTTATGATGCCAAGAAAGTTTGTGGAGAAATACGGAGAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAAAAGTGATGGAAAAATATGCTTTGAAAAGGGTTGGAAAAAATTTGCAGAGCATCACTCTCTAGCGCATGGCCATCTTCTGGTTTTCAAATATCTAAGAAATTCTCGTTTTCTTGTGCACATCTTTGAAAAGAGTGCCTTAGAGATAAATTACCCTTTACAAAGAGTTTCAGCTAAAGCTAACAGGGTCTCAAATGGTCAAGGAAATAAACTTCCAAATGGTGAAAATTGTAGAGCAGCTCAAAAGAGAAAAGTTAACTCCTTTGAACTTCATCAACCACGTGAAATCGGAAGCAGTAGTTGTTTTAGAGTTATACCGTCTATGTTTGGTATGAGACACTTCGATATGGAAAAGAAGAAGGGAGATATTTATTTTCAGGTGTTAACTAGTGGAAGAGTTTGGTCTGCAAGGTATATAATTAAGATGAGCAGAACAACATTTAAATTTGAACTCACAGGTGGATGGAAAGCATTTGCAAAGGACAATAACTTAAAAGTTGGTGATGTTTGTAACTTTGAGCTCATCTCTAGCACTTCACTTACGTTCACTGTTCACATCTTTAGAGAGGCAGAAAATGACAACACAAATTGTTCAATATCTCAAAAATCTTACCGAATAAAAGAAGAAGATATTCAAGGATGA
Protein sequence

>MsaT037152.1

MVKEINLQMVKIVEHLKREKITPLNFINRMKLEAVVVLQLENFKSKQYSLMASETNELKPIHFFKIILAQNLHEGKLMIPKKFVEKYGEGLSNAIYLKTPNGAKWNLNLVNSDGKIWFEKGWIEFAEHHSLAHGHLLLFRYEKTSLFEVHIFDKSALEINYPSQSFAAKKVSSGQKIKANSSFEFHQPCEIGSNGCVEVEKLQKVATLNHAGRESRGKQVITAKKVTTLERAQSFKTCNPSFLVVMRASYVKHRFLLNIPRMFGNRHFDLDQKRGDVYLQVLNEGVWPAKYLIRMTLTGPHVRLSGGWKAFAEDNNLKVGDVCKFELISSTILTFIYSLMASEMVRSVHFLKIIRTQNLHKGKLMMPRKFVEKYGEGLSNAIYLKTPNGAKWKLNLVKSDGKICFEKGWKKFAEHHSLAHGHLLVFKYLRNSRFLVHIFEKSALEINYPLQRVSAKANRVSNGQGNKLPNGENCRAAQKRKVNSFELHQPREIGSSSCFRVIPSMFGMRHFDMEKKKGDIYFQVLTSGRVWSARYIIKMSRTTFKFELTGGWKAFAKDNNLKVGDVCNFELISSTSLTFTVHIFREAENDNTNCSISQKSYRIKEEDIQG*