AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037157 MtrunA17_Chr7g0233571 69.591 171 46 2 1 168 1 168 9.98e-83 243
MsaG037157 MtrunA17_Chr7g0233561 69.643 168 51 0 1 168 6 173 3.69e-82 245
MsaG037157 MtrunA17_Chr3g0131891 70.238 168 44 1 1 168 7 168 9.01e-81 242
MsaG037157 MtrunA17_Chr7g0233541 76.923 143 30 2 28 168 1 142 6.51e-73 221
MsaG037157 MtrunA17_Chr7g0233521 73.188 138 23 1 28 165 1 124 1.27e-66 204
MsaG037157 MtrunA17_Chr7g0233531 73.529 136 29 2 28 163 1 129 1.95e-65 201
MsaG037157 MtrunA17_Chr8g0390841 41.509 159 88 3 11 168 37 191 1.60e-35 129
MsaG037157 MtrunA17_Chr5g0419201 44.348 115 61 1 9 120 12 126 7.94e-32 112
MsaG037157 MtrunA17_Chr3g0103191 50.485 103 49 1 11 113 10 110 9.42e-32 114
MsaG037157 MtrunA17_Chr3g0103151 49.038 104 51 1 11 114 10 111 1.05e-30 109
MsaG037157 MtrunA17_Chr1g0163071 52.941 85 40 0 28 112 1 85 1.62e-29 109
MsaG037157 MtrunA17_Chr1g0208771 54.217 83 38 0 29 111 13 95 7.90e-29 110
MsaG037157 MtrunA17_Chr3g0103181 49.505 101 49 1 11 111 10 108 2.47e-28 106
MsaG037157 MtrunA17_Chr6g0467351 41.379 145 73 5 11 145 13 155 2.90e-25 97.1
MsaG037157 MtrunA17_Chr1g0162441 41.584 101 59 0 13 113 7 107 2.91e-24 97.4
MsaG037157 MtrunA17_Chr3g0103171 47.674 86 45 0 29 114 6 91 5.29e-24 94.4
MsaG037157 MtrunA17_Chr4g0033961 40.385 104 62 0 10 113 3 106 6.19e-24 96.7
MsaG037157 MtrunA17_Chr1g0163101 36.434 129 77 3 9 135 13 138 2.49e-23 90.9
MsaG037157 MtrunA17_Chr1g0163251 45.192 104 51 2 12 115 8 105 2.44e-22 92.0
MsaG037157 MtrunA17_Chr1g0163131 36.434 129 79 2 7 135 12 137 6.20e-22 89.7
MsaG037157 MtrunA17_Chr4g0067431 45.631 103 55 1 11 112 13 115 6.21e-22 90.1
MsaG037157 MtrunA17_Chr1g0171611 41.748 103 58 1 13 115 9 109 9.69e-22 89.0
MsaG037157 MtrunA17_Chr3g0077461 39.423 104 63 0 9 112 233 336 3.15e-21 89.4
MsaG037157 MtrunA17_Chr3g0077461 29.710 138 85 3 11 145 2 130 7.03e-18 79.7
MsaG037157 MtrunA17_Chr1g0163191 42.308 104 54 2 12 115 8 105 3.53e-21 84.7
MsaG037157 MtrunA17_Chr1g0163231 40.777 103 59 1 13 115 9 109 6.24e-21 87.0
MsaG037157 MtrunA17_Chr3g0077411 35.849 106 68 0 7 112 19 124 1.04e-20 87.8
MsaG037157 MtrunA17_Chr3g0077411 36.190 105 66 1 9 112 258 362 1.08e-20 87.8
MsaG037157 MtrunA17_Chr1g0163181 37.097 124 75 2 22 144 14 135 2.54e-20 86.7
MsaG037157 MtrunA17_Chr1g0163121 48.315 89 43 2 27 115 9 94 3.26e-20 81.6
MsaG037157 MtrunA17_Chr1g0162411 38.235 102 61 1 13 114 7 106 3.73e-20 86.3
MsaG037157 MtrunA17_Chr3g0077381 31.783 129 84 2 18 145 25 150 6.42e-20 85.5
MsaG037157 MtrunA17_Chr3g0077381 35.577 104 67 0 9 112 243 346 2.36e-16 75.5
MsaG037157 MtrunA17_Chr1g0163241 42.708 96 48 2 29 119 23 116 2.75e-19 80.1
MsaG037157 MtrunA17_Chr4g0009491 32.500 120 77 2 27 145 11 127 4.95e-19 82.8
MsaG037157 MtrunA17_Chr4g0009491 36.538 104 66 0 9 112 221 324 3.95e-18 80.5
MsaG037157 MtrunA17_Chr1g0163221 31.461 178 105 4 7 168 5 181 7.82e-19 82.0
MsaG037157 MtrunA17_Chr3g0077361 30.519 154 91 4 3 147 30 176 2.21e-18 81.3
MsaG037157 MtrunA17_Chr3g0077361 35.135 74 48 0 9 82 285 358 9.12e-11 59.3
MsaG037157 MtrunA17_Chr1g0163261 33.775 151 93 2 13 158 9 157 4.41e-18 79.3
MsaG037157 MtrunA17_Chr1g0163141 40.000 100 57 2 27 126 9 105 8.03e-18 76.6
MsaG037157 MtrunA17_Chr1g0163061 40.777 103 60 1 11 112 13 115 2.20e-17 77.0
MsaG037157 MtrunA17_Chr3g0077451 30.435 115 71 1 27 141 10 115 2.42e-17 76.3
MsaG037157 MtrunA17_Chr1g0163201 41.379 87 49 1 29 115 19 103 3.21e-17 77.8
MsaG037157 MtrunA17_Chr5g0429931 33.607 122 76 2 29 146 11 131 3.49e-17 76.3
MsaG037157 MtrunA17_Chr1g0162161 35.514 107 66 2 28 134 3 106 1.67e-16 72.4
MsaG037157 MtrunA17_Chr6g0476181 40.404 99 56 2 28 126 1 96 3.21e-16 71.6
MsaG037157 MtrunA17_Chr1g0163081 39.583 96 55 2 28 123 5 97 2.14e-15 69.3
MsaG037157 MtrunA17_Chr1g0154101 33.696 92 61 0 11 102 23 114 3.99e-14 69.3
MsaG037157 MtrunA17_Chr1g0154101 32.258 93 61 1 11 103 182 272 2.43e-13 67.0
MsaG037157 MtrunA17_Chr1g0154071 31.496 127 82 2 9 132 153 277 4.07e-14 68.9
MsaG037157 MtrunA17_Chr1g0154071 28.866 97 68 1 11 107 26 121 6.74e-11 59.7
MsaG037157 MtrunA17_Chr1g0154161 30.928 97 67 0 11 107 28 124 6.80e-14 67.8
MsaG037157 MtrunA17_Chr1g0154211 33.333 93 61 1 11 103 21 112 1.59e-13 67.4
MsaG037157 MtrunA17_Chr1g0154251 32.258 93 62 1 11 103 25 116 1.70e-13 67.4
MsaG037157 MtrunA17_Chr1g0154041 33.663 101 66 1 8 108 11 110 7.58e-13 65.5
MsaG037157 MtrunA17_Chr1g0163161 36.082 97 57 3 41 135 1 94 9.90e-13 62.0
MsaG037157 MtrunA17_Chr1g0154171 31.579 95 64 1 11 105 21 114 1.57e-12 64.3
MsaG037157 MtrunA17_Chr1g0154131 31.959 97 64 1 11 107 182 276 3.56e-12 63.5
MsaG037157 MtrunA17_Chr1g0154191 30.108 93 65 0 11 103 20 112 1.56e-11 61.6
MsaG037157 MtrunA17_Chr1g0154241 29.897 97 67 1 11 107 20 115 2.50e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037157 AT3G18990.1 45.098 102 56 0 13 114 6 107 2.69e-25 99.8
MsaG037157 AT3G18990.2 45.098 102 56 0 13 114 6 107 3.56e-25 99.8
MsaG037157 AT1G49475.1 41.739 115 66 1 6 120 27 140 2.36e-24 94.0
MsaG037157 AT4G01580.1 40.187 107 64 0 6 112 23 129 1.13e-22 89.7
MsaG037157 AT3G18960.2 40.187 107 64 0 6 112 23 129 3.39e-22 88.6
MsaG037157 AT3G18960.1 40.187 107 64 0 6 112 23 129 4.55e-22 88.6
MsaG037157 AT3G18960.3 43.182 88 50 0 29 116 9 96 4.88e-18 77.0

Find 34 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTACAATCCTCATTATT+TGG 0.147186 7:-52793762 None:intergenic
TGCAAAATGGAAATTAAATT+TGG 0.194062 7:+52793530 MsaT037157.1:CDS
CCACAAGAGTTGAAGCTAAA+AGG 0.209640 7:+52793712 MsaT037157.1:CDS
TATTTCTCCACAAACTTTCT+TGG 0.217927 7:-52793462 None:intergenic
AGAAAGTTTGTGGAGAAATA+TGG 0.293086 7:+52793465 MsaT037157.1:CDS
TTTCATCAACCACGTGAAAT+AGG 0.293751 7:+52793819 MsaT037157.1:CDS
AGACTCCAAATGGTGCAAAA+TGG 0.337543 7:+52793517 MsaT037157.1:CDS
GGAAGGGTAACTTATCTCTA+AGG 0.432576 7:-52793691 None:intergenic
TGGCAAGATATGGTTTGAAA+AGG 0.433004 7:+52793563 MsaT037157.1:CDS
AGCAGTGGTTGTGTTAAAGT+TGG 0.434623 7:+52793843 MsaT037157.1:CDS
CACAAGAGTTGAAGCTAAAA+GGG 0.438433 7:+52793713 MsaT037157.1:CDS
GATGATGCCAAGAAAGTTTG+TGG 0.445895 7:+52793455 MsaT037157.1:intron
CATATTTGAAAAGCAGAAGA+TGG 0.450028 7:-52793626 None:intergenic
AGATATGGTTTGAAAAGGGT+TGG 0.475702 7:+52793568 MsaT037157.1:CDS
CTTCACCTCAGGAATTTCAC+TGG 0.477594 7:-52791894 None:intergenic
GCAGTGGTTGTGTTAAAGTT+GGG 0.493563 7:+52793844 MsaT037157.1:CDS
ATTTGGAGTCTTTAGATACA+TGG 0.499172 7:-52793505 None:intergenic
TTAGCTTCAACTCTTGTGGA+AGG 0.514301 7:-52793708 None:intergenic
ATGTATCTAAAGACTCCAAA+TGG 0.514402 7:+52793507 MsaT037157.1:CDS
GGCAAGATATGGTTTGAAAA+GGG 0.522590 7:+52793564 MsaT037157.1:CDS
GGTTGATGAAATTCAAATGA+GGG 0.525738 7:-52793807 None:intergenic
AAGTGGATTGACTTCACCTC+AGG 0.534487 7:-52791905 None:intergenic
CTATAAATCTCCAAATAATG+AGG 0.558294 7:+52793752 MsaT037157.1:CDS
TGGTTGATGAAATTCAAATG+AGG 0.559107 7:-52793808 None:intergenic
TAGCTTCAACTCTTGTGGAA+GGG 0.568047 7:-52793707 None:intergenic
TTTGTGGAGAAATATGGAGA+AGG 0.578281 7:+52793471 MsaT037157.1:CDS
CAGCATCACTCACTAGCTCA+TGG 0.601771 7:+52793603 MsaT037157.1:CDS
CTTACTCACAGCATTCATGA+AGG 0.605183 7:+52791941 MsaT037157.1:CDS
CCACTGCTTCCTATTTCACG+TGG 0.606234 7:-52793828 None:intergenic
GTTGATGAAATTCAAATGAG+GGG 0.615052 7:-52793806 None:intergenic
CCACGTGAAATAGGAAGCAG+TGG 0.619437 7:+52793828 MsaT037157.1:CDS
GAAGCTAAAAGGGTCTCGAA+TGG 0.643697 7:+52793723 MsaT037157.1:CDS
TTAAAGTTGGGCAATCACAA+AGG 0.676682 7:+52793856 MsaT037157.1:CDS
CATGGCCAGTGAAATTCCTG+AGG 0.703012 7:+52791889 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 52791890 52793881 52791890 ID=MsaG037157
Chr7 mRNA 52791890 52793881 52791890 ID=MsaT037157.1;Parent=MsaG037157
Chr7 exon 52791890 52791970 52791890 ID=MsaT037157.1.exon1;Parent=MsaT037157.1
Chr7 CDS 52791890 52791970 52791890 ID=cds.MsaT037157.1;Parent=MsaT037157.1
Chr7 exon 52793456 52793881 52793456 ID=MsaT037157.1.exon2;Parent=MsaT037157.1
Chr7 CDS 52793456 52793881 52793456 ID=cds.MsaT037157.1;Parent=MsaT037157.1
Gene Sequence

>MsaT037157.1

ATGGCCAGTGAAATTCCTGAGGTGAAGTCAATCCACTTTTTCAAGATCATACTTACTCACAGCATTCATGAAGGAAAGCTTATGATGCCAAGAAAGTTTGTGGAGAAATATGGAGAAGGTTTATCAAATGCCATGTATCTAAAGACTCCAAATGGTGCAAAATGGAAATTAAATTTGGTAAAAAGTGATGGCAAGATATGGTTTGAAAAGGGTTGGAAAGAATTTGCACAGCATCACTCACTAGCTCATGGCCATCTTCTGCTTTTCAAATATGAAAGAACATCCCATTTTTATGTGCACGTTTTTGAAAAGAGTGCCTTAGAGATAAGTTACCCTTCCACAAGAGTTGAAGCTAAAAGGGTCTCGAATGGTCAAAGCTATAAATCTCCAAATAATGAGGATTGTAGAGCATGTCAAAAGAGAAAAGCTAACCCCTCATTTGAATTTCATCAACCACGTGAAATAGGAAGCAGTGGTTGTGTTAAAGTTGGGCAATCACAAAGGTAG
Protein sequence

>MsaT037157.1

MASEIPEVKSIHFFKIILTHSIHEGKLMMPRKFVEKYGEGLSNAMYLKTPNGAKWKLNLVKSDGKIWFEKGWKEFAQHHSLAHGHLLLFKYERTSHFYVHVFEKSALEISYPSTRVEAKRVSNGQSYKSPNNEDCRACQKRKANPSFEFHQPREIGSSGCVKVGQSQR*