AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037172

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037172 MtrunA17_Chr7g0223591 86.139 303 39 3 253 555 45 344 1.50e-170 501
MsaG037172 MtrunA17_Chr7g0223561 90.494 263 23 2 314 576 1 261 1.34e-156 462
MsaG037172 MtrunA17_Chr7g0223551 86.716 271 31 4 637 902 1 271 2.64e-155 458
MsaG037172 MtrunA17_Chr7g0233571 91.855 221 18 0 1 221 1 221 1.08e-145 430
MsaG037172 MtrunA17_Chr4g0041921 44.919 679 290 18 280 903 164 813 2.78e-144 449
MsaG037172 MtrunA17_Chr7g0215611 65.875 337 62 9 266 555 56 386 4.21e-127 389
MsaG037172 MtrunA17_Chr7g0233541 63.519 233 78 4 25 255 1 228 9.02e-96 303
MsaG037172 MtrunA17_Chr3g0131891 62.162 259 81 7 1 255 7 252 2.63e-94 300
MsaG037172 MtrunA17_Chr7g0233561 58.527 258 101 4 4 260 12 264 7.03e-93 296
MsaG037172 MtrunA17_Chr7g0233521 58.874 231 76 3 25 255 1 212 1.54e-85 275
MsaG037172 MtrunA17_Chr7g0215621 55.973 293 74 5 633 889 4 277 5.03e-78 255
MsaG037172 MtrunA17_Chr4g0075891 39.669 484 190 17 469 902 91 522 3.14e-75 256
MsaG037172 MtrunA17_Chr7g0233531 54.545 231 86 5 25 255 1 212 5.97e-72 238
MsaG037172 MtrunA17_Chr7g0223581 91.860 86 7 0 637 722 1 86 6.16e-47 162
MsaG037172 MtrunA17_Chr5g0413891 41.036 251 109 6 687 902 15 261 4.86e-42 156
MsaG037172 MtrunA17_Chr2g0319471 42.727 220 112 7 300 513 186 397 2.64e-38 150
MsaG037172 MtrunA17_Chr1g0163071 34.646 254 145 5 25 261 1 250 8.77e-37 140
MsaG037172 MtrunA17_Chr4g0041891 51.266 158 67 5 300 455 165 314 8.87e-35 136
MsaG037172 MtrunA17_Chr8g0390841 32.143 280 163 10 10 271 39 309 4.23e-32 132
MsaG037172 MtrunA17_Chr1g0208771 32.377 244 136 7 26 252 13 244 6.46e-30 124
MsaG037172 MtrunA17_Chr3g0103191 30.682 264 137 6 7 250 9 246 1.20e-29 118
MsaG037172 MtrunA17_Chr3g0103151 49.020 102 50 1 7 108 9 108 7.71e-27 107
MsaG037172 MtrunA17_Chr3g0103181 31.349 252 140 9 2 244 4 231 1.03e-26 110
MsaG037172 MtrunA17_Chr5g0419201 35.912 181 102 5 10 182 16 190 6.21e-26 105
MsaG037172 MtrunA17_Chr8g0368851 34.098 305 118 11 595 824 1 297 5.39e-24 103
MsaG037172 MtrunA17_Chr8g0368841 57.471 87 33 3 469 554 87 170 2.26e-22 95.5
MsaG037172 MtrunA17_Chr1g0163261 33.061 245 138 9 5 246 4 225 2.31e-22 99.0
MsaG037172 MtrunA17_Chr5g0413901 80.769 52 10 0 503 554 1 52 2.60e-22 91.3
MsaG037172 MtrunA17_Chr4g0067431 29.528 254 166 5 9 254 14 262 1.31e-20 94.0
MsaG037172 MtrunA17_Chr4g0033961 40.909 110 63 1 5 112 1 110 2.05e-20 95.1
MsaG037172 MtrunA17_Chr1g0163231 31.602 231 123 9 7 232 6 206 2.59e-20 92.8
MsaG037172 MtrunA17_Chr6g0467351 38.843 121 71 2 9 126 14 134 3.37e-20 90.5
MsaG037172 MtrunA17_Chr1g0163181 31.982 222 132 7 19 232 14 224 3.50e-20 94.7
MsaG037172 MtrunA17_Chr3g0103171 31.034 203 106 6 26 218 6 184 5.90e-20 90.9
MsaG037172 MtrunA17_Chr1g0162441 40.000 130 75 2 6 134 3 130 6.07e-20 93.6
MsaG037172 MtrunA17_Chr1g0163251 46.296 108 51 3 1 108 1 101 3.08e-19 91.3
MsaG037172 MtrunA17_Chr1g0171611 42.157 102 57 1 7 108 6 105 3.39e-19 89.4
MsaG037172 MtrunA17_Chr1g0163241 38.732 142 74 4 26 156 23 162 3.87e-18 82.8
MsaG037172 MtrunA17_Chr1g0163201 31.628 215 122 6 26 232 19 216 5.96e-18 87.4
MsaG037172 MtrunA17_Chr1g0163191 45.556 90 47 1 19 108 14 101 6.79e-18 82.0
MsaG037172 MtrunA17_Chr3g0077411 37.864 103 64 0 9 111 24 126 2.51e-17 86.3
MsaG037172 MtrunA17_Chr3g0077411 30.935 139 95 1 9 146 261 399 2.00e-15 80.5
MsaG037172 MtrunA17_Chr1g0163101 35.664 143 82 4 9 145 16 154 4.76e-17 80.1
MsaG037172 MtrunA17_Chr3g0077461 34.400 125 82 0 9 133 236 360 4.93e-17 85.1
MsaG037172 MtrunA17_Chr3g0077461 39.535 86 52 0 26 111 23 108 1.42e-15 80.5
MsaG037172 MtrunA17_Chr3g0077451 34.109 129 82 2 24 150 10 137 4.35e-16 79.0
MsaG037172 MtrunA17_Chr5g0429931 31.783 129 73 2 13 141 1 114 1.04e-15 77.8
MsaG037172 MtrunA17_Chr1g0163221 37.255 102 63 1 9 109 10 111 1.09e-15 79.7
MsaG037172 MtrunA17_Chr1g0154131 27.530 247 152 5 23 259 195 424 1.44e-15 80.5
MsaG037172 MtrunA17_Chr1g0163121 46.067 89 45 2 24 112 9 94 2.08e-15 74.3
MsaG037172 MtrunA17_Chr1g0162411 41.053 95 56 0 10 104 7 101 9.36e-15 77.8
MsaG037172 MtrunA17_Chr3g0077381 34.259 108 68 1 24 131 34 138 2.08e-14 76.6
MsaG037172 MtrunA17_Chr3g0077381 32.000 125 85 0 9 133 246 370 1.66e-12 70.9
MsaG037172 MtrunA17_Chr1g0163061 38.462 104 63 1 9 111 14 117 3.08e-14 73.9
MsaG037172 MtrunA17_Chr4g0009491 32.800 125 84 0 9 133 224 348 5.67e-14 75.1
MsaG037172 MtrunA17_Chr4g0009491 32.283 127 75 2 24 150 11 126 6.09e-14 75.1
MsaG037172 MtrunA17_Chr1g0163131 36.585 123 75 2 9 131 17 136 5.90e-14 73.9
MsaG037172 MtrunA17_Chr1g0162161 36.449 107 65 2 25 131 3 106 7.00e-14 70.5
MsaG037172 MtrunA17_Chr1g0154161 24.535 269 137 7 9 252 29 256 8.68e-14 73.6
MsaG037172 MtrunA17_Chr1g0163141 43.820 89 47 2 24 112 9 94 1.19e-13 70.5
MsaG037172 MtrunA17_Chr1g0154251 39.130 92 55 1 9 100 26 116 1.88e-13 73.9
MsaG037172 MtrunA17_Chr1g0154041 26.102 295 170 10 9 259 15 305 2.87e-13 73.6
MsaG037172 MtrunA17_Chr1g0154101 29.891 184 106 5 9 191 183 344 3.02e-13 73.9
MsaG037172 MtrunA17_Chr1g0154101 38.462 91 56 0 9 99 24 114 1.13e-12 72.0
MsaG037172 MtrunA17_Chr1g0154211 38.776 98 59 1 3 100 16 112 3.10e-13 72.8
MsaG037172 MtrunA17_Chr1g0154171 23.333 270 159 6 21 252 33 292 7.08e-13 71.2
MsaG037172 MtrunA17_Chr1g0154071 23.507 268 163 6 24 252 169 433 1.25e-12 71.2
MsaG037172 MtrunA17_Chr1g0154071 34.375 96 62 1 9 104 27 121 2.32e-11 67.4
MsaG037172 MtrunA17_Chr1g0154111 23.958 288 170 7 9 252 25 307 1.53e-12 71.2
MsaG037172 MtrunA17_Chr4g0075901 52.941 68 21 2 488 555 1 57 1.82e-12 63.5
MsaG037172 MtrunA17_Chr3g0077361 29.371 143 89 3 9 150 39 170 3.41e-12 70.1
MsaG037172 MtrunA17_Chr1g0154191 36.735 98 62 0 3 100 15 112 4.98e-12 68.6
MsaG037172 MtrunA17_Chr1g0163081 39.583 96 55 2 25 120 5 97 9.03e-12 63.9
MsaG037172 MtrunA17_Chr1g0154241 34.375 96 62 1 9 104 21 115 1.15e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037172 AT4G21880.2 34.421 613 338 15 324 895 173 762 6.47e-87 295
MsaG037172 AT4G21880.1 34.421 613 338 15 324 895 217 806 7.86e-87 296
MsaG037172 AT4G04790.2 33.441 622 357 16 310 894 47 648 4.77e-85 287
MsaG037172 AT4G04790.1 33.441 622 357 16 310 894 184 785 3.18e-84 288
MsaG037172 AT4G04790.3 33.441 622 357 16 310 894 180 781 5.35e-84 287
MsaG037172 AT3G18990.1 30.872 298 148 10 6 255 2 289 2.11e-26 112
MsaG037172 AT3G18990.2 30.872 298 148 10 6 255 2 289 5.80e-26 111
MsaG037172 AT4G01580.1 34.483 145 93 1 4 148 24 166 6.37e-21 92.0
MsaG037172 AT3G18960.1 40.000 110 66 0 4 113 24 133 7.71e-21 92.4
MsaG037172 AT3G18960.2 40.000 110 66 0 4 113 24 133 8.77e-21 91.7
MsaG037172 AT1G49475.1 38.889 108 65 1 10 117 34 140 1.66e-18 85.1
MsaG037172 AT3G18960.3 44.318 88 49 0 26 113 9 96 9.33e-18 82.4
MsaG037172 AT4G33280.2 38.614 101 58 2 8 104 18 118 3.91e-11 65.5
MsaG037172 AT4G33280.1 25.424 236 163 6 8 233 18 250 5.97e-11 65.5

Find 153 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTTGAATAAAGCTAATT+TGG 0.210052 7:-52964694 MsaT037172.1:CDS
CGAAGATGTGCACTTCAAAA+TGG 0.226194 7:+52975235 None:intergenic
ATTCATCTTAGGTTATTAAA+TGG 0.226202 7:-52973892 MsaT037172.1:CDS
TTTAATCAACCATGTGAAAT+TGG 0.235269 7:-52975066 MsaT037172.1:CDS
GGGTCCACACATTTGCAATT+TGG 0.242362 7:-52964807 MsaT037172.1:CDS
TATTTCTCCACAAACTTTCT+TGG 0.252264 7:+52975432 None:intergenic
CTGTGTTAAAGTTGGGAAAT+TGG 0.261586 7:-52975034 MsaT037172.1:CDS
AAGTTGGTCACCTCTAAATT+TGG 0.262612 7:+52967519 None:intergenic
ATGCTTGTGAGTATATTAAA+AGG 0.264608 7:-52967974 MsaT037172.1:CDS
TTTGGTAAAAGACACTTATT+AGG 0.278617 7:-52973937 MsaT037172.1:CDS
TCAAATATATTCAAATTATT+AGG 0.280088 7:-52967725 MsaT037172.1:CDS
AGAAAGTTTGTGGAGAAATA+TGG 0.293086 7:-52975429 MsaT037172.1:CDS
ACACCATTAGAGAGAGATTT+GGG 0.300564 7:-52966736 MsaT037172.1:CDS
TTTATTCAAATCTCTTGATT+TGG 0.308939 7:+52964704 None:intergenic
GGTTATTAAATGGGAGAGTT+TGG 0.317803 7:-52973882 MsaT037172.1:CDS
TCTTCTTCTAGTTCTAGTTC+TGG 0.328382 7:+52968167 None:intergenic
GCAATTTGGATTGGACTTGC+TGG 0.332582 7:-52964793 MsaT037172.1:CDS
AAGATCGTTGGATACCCTTT+TGG 0.336100 7:+52975167 None:intergenic
TTCAATATCAATTTCGCGAT+TGG 0.345428 7:-52968051 MsaT037172.1:CDS
AAAAGACACTTATTAGGTTT+AGG 0.347950 7:-52973931 MsaT037172.1:CDS
TTCCTAATTAGATACCTTGC+AGG 0.351726 7:+52973859 None:intergenic
TAGAATGTATCACGTACTTT+TGG 0.352753 7:-52964538 MsaT037172.1:CDS
GACACCATTAGAGAGAGATT+TGG 0.355285 7:-52966737 MsaT037172.1:CDS
AATCTGCATATGCATGAATT+TGG 0.359386 7:+52965635 None:intergenic
ACCTCCGAATGATGAAGATT+TGG 0.360032 7:-52975136 MsaT037172.1:CDS
CAGAACATACCAAGTGAGTT+TGG 0.360251 7:-52973955 MsaT037172.1:CDS
CAAATGGCGTGACCCCTTCT+TGG 0.360786 7:+52966228 None:intergenic
TGAATAAAGCTAATTTGGTT+TGG 0.362065 7:-52964689 MsaT037172.1:CDS
AGCAAGGAGATATTCATCTT+AGG 0.368678 7:-52973903 MsaT037172.1:CDS
AGTTCTAGTTCTGGTTGAAC+TGG 0.373388 7:+52968176 None:intergenic
CACACATTTGCAATTTGGAT+TGG 0.379956 7:-52964802 MsaT037172.1:CDS
CAAGAAGGGGTCACGCCATT+TGG 0.387051 7:-52966227 MsaT037172.1:CDS
ATTTAGCATTCCAAATTTAG+AGG 0.389184 7:-52967529 MsaT037172.1:intron
ATTTCTCCACAAACTTTCTT+GGG 0.389419 7:+52975433 None:intergenic
GCAACAACAACGTTTCGAAT+TGG 0.390614 7:-52968261 MsaT037172.1:CDS
CGGAGTTGAGTGGCGAAGAT+TGG 0.395356 7:-52965205 MsaT037172.1:CDS
AGTGGCGAAGATTGGGTTCA+AGG 0.406061 7:-52965197 MsaT037172.1:CDS
TGCAAAGTGGAGATTAGATT+TGG 0.413094 7:-52975364 MsaT037172.1:CDS
TCAATATCAATTTCGCGATT+GGG 0.413807 7:-52968050 MsaT037172.1:CDS
CATTGATGCTGCTTGTAATC+TGG 0.414628 7:-52974679 MsaT037172.1:intron
CGAAATGTTTATGCGGTACT+CGG 0.418807 7:-52965171 MsaT037172.1:CDS
TTCTTGGTCCCTACATATGC+TGG 0.425412 7:+52966244 None:intergenic
TCCAAAGAGTTGAAGCCAAA+AGG 0.428396 7:-52975182 MsaT037172.1:CDS
TTCATCTTAGGTTATTAAAT+GGG 0.428424 7:-52973891 MsaT037172.1:CDS
ATGACATGTTGATGTGGATA+AGG 0.432395 7:-52968022 MsaT037172.1:CDS
TGAATTTGAGAAAGCAAAAC+AGG 0.437605 7:-52965608 MsaT037172.1:intron
AAATATGGTTTCAAAAGGGT+TGG 0.440218 7:-52975326 MsaT037172.1:CDS
GATGATCCCAAGAAAGTTTG+TGG 0.440968 7:-52975439 MsaT037172.1:CDS
TCTTCCAAATCTTCATCATT+CGG 0.441815 7:+52975132 None:intergenic
GCAATAGCTGTGTTAAAGTT+GGG 0.451550 7:-52975041 MsaT037172.1:CDS
GGAGTTGAGTGGCGAAGATT+GGG 0.455800 7:-52965204 MsaT037172.1:CDS
AGCAATAGCTGTGTTAAAGT+TGG 0.455850 7:-52975042 MsaT037172.1:CDS
ATTTCAATTAACCACTTCAC+TGG 0.456205 7:+52965476 None:intergenic
AAAAGGGTATCCAACGATCT+TGG 0.458573 7:-52975165 MsaT037172.1:CDS
TTACAAGCTTTCCTTGATGA+AGG 0.460972 7:+52975590 None:intergenic
CCAAAGAGTTGAAGCCAAAA+GGG 0.465061 7:-52975181 MsaT037172.1:CDS
TTGATTGTCAATATAGCAAA+AGG 0.465452 7:+52965925 None:intergenic
TTGATTTGGCACAAGCAGAT+AGG 0.469420 7:+52964718 None:intergenic
TGACATGTTGATGTGGATAA+GGG 0.469858 7:-52968021 MsaT037172.1:CDS
GCTGTATACAATACACCAAA+TGG 0.472205 7:+52966212 None:intergenic
CTCACAAGCATCGCGGATCA+TGG 0.474289 7:+52967986 None:intergenic
ATGAGACATAAAACAGCTCT+AGG 0.475843 7:+52965356 None:intergenic
TACCCCAAATCTCTCTCTAA+TGG 0.478000 7:+52966733 None:intergenic
ATGTAGTGCAGAAGATGATC+TGG 0.480251 7:-52967583 MsaT037172.1:intron
TGGCTTCAACTCTTTGGAAA+GGG 0.481840 7:+52975187 None:intergenic
GAAGATGTGCACTTCAAAAT+GGG 0.484263 7:+52975236 None:intergenic
TTGGAGGAAATCAAGAAAGC+TGG 0.486524 7:-52965392 MsaT037172.1:CDS
CATATTTGAAAAGCAGAAGA+TGG 0.487743 7:+52975268 None:intergenic
AATCTCCACTTTGCACCACT+TGG 0.487838 7:+52975372 None:intergenic
TGGAAAGAATATGCATTGGC+TGG 0.492664 7:-52964669 MsaT037172.1:CDS
TATGAAGAGATGGAACAATC+TGG 0.495912 7:-52965688 MsaT037172.1:CDS
TAGAGAGACTAACAATTGAA+AGG 0.499434 7:-52968322 MsaT037172.1:CDS
ATGCTGGAGCATTTGTGCAC+CGG 0.503432 7:+52966260 None:intergenic
CATAGAGTTTGCTCTGTCAA+AGG 0.505840 7:+52974746 None:intergenic
CTTGTAGTATTATGAAGAGA+TGG 0.505940 7:-52965698 MsaT037172.1:intron
ATAATTGACATGGGAGATGT+TGG 0.507156 7:-52968119 MsaT037172.1:CDS
AGGTTTATTGCCAAGATCGT+TGG 0.509044 7:+52975155 None:intergenic
GAGTTGTTTAAACATTTCAA+TGG 0.512869 7:+52965046 None:intergenic
TTGGCTTCAACTCTTTGGAA+AGG 0.513756 7:+52975186 None:intergenic
TAAAGTTGGGAAATTGGAAA+AGG 0.514132 7:-52975028 MsaT037172.1:CDS
GTTGATAAAATGTGTGAAGC+AGG 0.514403 7:-52966959 MsaT037172.1:CDS
GCATATGTAGGGACCAAGAA+GGG 0.515295 7:-52966241 MsaT037172.1:CDS
TGCTGGAGCATTTGTGCACC+GGG 0.516820 7:+52966261 None:intergenic
TGAAATGTTTAAACAACTCA+AGG 0.520211 7:-52965042 MsaT037172.1:CDS
AGAGAGACTAACAATTGAAA+GGG 0.520948 7:-52968321 MsaT037172.1:CDS
GTCTCATCTCTTGCATCACA+CGG 0.523873 7:-52965440 MsaT037172.1:CDS
CGCACGACAGCAATAAAAGA+TGG 0.524177 7:+52974711 None:intergenic
TACAGATTGCATTACATGAC+TGG 0.530721 7:+52966925 None:intergenic
CAAGCAGCATCAATGTATGA+TGG 0.531742 7:+52974687 None:intergenic
ACAAATGCTCCAGCATATGT+AGG 0.535178 7:-52966253 MsaT037172.1:CDS
ATGGGAGAGTTTGGCCTGCA+AGG 0.535413 7:-52973873 MsaT037172.1:intron
ATTATGACGAAGATTGCTAC+AGG 0.543345 7:-52964641 MsaT037172.1:CDS
CTCCAACAAAACAAGTGCCT+CGG 0.544653 7:+52965409 None:intergenic
ATTTGAAAATATTTCATCTG+TGG 0.547796 7:+52967742 None:intergenic
TGCTTGTGAGTATATTAAAA+GGG 0.549777 7:-52967973 MsaT037172.1:CDS
AATTGGAGATTGAGAAGGCT+AGG 0.552721 7:-52968244 MsaT037172.1:CDS
AAGTATCATTCTTTATCTCA+AGG 0.553311 7:-52975291 MsaT037172.1:CDS
ATATATTCAAATTATTAGGA+AGG 0.554147 7:-52967721 MsaT037172.1:CDS
TTTACACGGCGAGTTGGAGA+GGG 0.556976 7:-52965243 MsaT037172.1:CDS
AAAGAGATTGAGAATTTGCA+TGG 0.557143 7:-52967264 MsaT037172.1:CDS
TCAATTATATACTTGAGAAG+TGG 0.559264 7:+52968134 None:intergenic
GGATGAGTGGCAAGACTAGA+CGG 0.559350 7:-52966775 MsaT037172.1:intron
AGCATATGTAGGGACCAAGA+AGG 0.559410 7:-52966242 MsaT037172.1:CDS
GATGAGTGGCAAGACTAGAC+GGG 0.563858 7:-52966774 MsaT037172.1:intron
CAAATGCTCCAGCATATGTA+GGG 0.563920 7:-52966252 MsaT037172.1:CDS
CTATTGCTTCCAATTTCACA+TGG 0.566631 7:+52975057 None:intergenic
TTTCGAATTGGAGATTGAGA+AGG 0.574478 7:-52968249 MsaT037172.1:CDS
GTTTACACGGCGAGTTGGAG+AGG 0.577781 7:-52965244 MsaT037172.1:CDS
TTTGTGGAGAAATATGGAGA+AGG 0.578281 7:-52975423 MsaT037172.1:CDS
AGCAGATTTGTGTTCTCCCG+AGG 0.579128 7:+52964515 None:intergenic
GTTATGTTTACACGGCGAGT+TGG 0.581675 7:-52965249 MsaT037172.1:CDS
AAAATTGTGGAATTCTAGAG+AGG 0.584254 7:-52968297 MsaT037172.1:CDS
TCCAAATCTTCATCATTCGG+AGG 0.588522 7:+52975135 None:intergenic
CAATCCAAATTGCAAATGTG+TGG 0.592711 7:+52964803 None:intergenic
GATTACCTTGAAAAGCCTCT+AGG 0.597456 7:+52973167 None:intergenic
TGTGATCAAAGCCTTCATCA+AGG 0.598883 7:-52975601 MsaT037172.1:CDS
GTTATTATGACATGTTGATG+TGG 0.599778 7:-52968028 MsaT037172.1:CDS
TAAGACATTGCGAAGGAACA+CGG 0.599807 7:+52964748 None:intergenic
GAACTAGAAGAAGAAACATA+CGG 0.604179 7:-52968158 MsaT037172.1:CDS
GTATTGCTGTGGTAAGAACA+AGG 0.611244 7:-52967193 MsaT037172.1:intron
TAGGATGTGTTATGTTTACA+CGG 0.611885 7:-52965257 MsaT037172.1:CDS
TACCTTGAAAAGCCTCTAGG+AGG 0.615390 7:+52973170 None:intergenic
TCTCATCTCTTGCATCACAC+GGG 0.615918 7:-52965439 MsaT037172.1:CDS
GAGGGGTCTTCTCCTACGCA+CGG 0.616383 7:-52965225 MsaT037172.1:CDS
TCTCAAGTATATAATTGACA+TGG 0.617377 7:-52968129 MsaT037172.1:CDS
GGTTTGGAAAGAATATGCAT+TGG 0.617747 7:-52964673 MsaT037172.1:CDS
GATTGCATTACATGACTGGA+GGG 0.621878 7:+52966929 None:intergenic
ACGAATCAAATTCAATTGTG+AGG 0.621986 7:+52968217 None:intergenic
TTACTATTCGAATGTTATGA+CGG 0.628963 7:-52967671 MsaT037172.1:intron
AGACTCCAAGTGGTGCAAAG+TGG 0.629081 7:-52975377 MsaT037172.1:CDS
CTCCTACGCACGGAGTTGAG+TGG 0.630400 7:-52965215 MsaT037172.1:CDS
CTCAAGTATATAATTGACAT+GGG 0.632252 7:-52968128 MsaT037172.1:CDS
TGAGACATAAAACAGCTCTA+GGG 0.635509 7:+52965357 None:intergenic
ATCAACGCATACCAGTGAAG+TGG 0.637290 7:-52965487 MsaT037172.1:CDS
TTGTGAAAGTGATGACCGCA+AGG 0.639107 7:-52967813 MsaT037172.1:CDS
CGCCACTCAACTCCGTGCGT+AGG 0.640967 7:+52965213 None:intergenic
CATCTGAAATCTGTGCCTTG+CGG 0.647291 7:+52967798 None:intergenic
AGATTGCATTACATGACTGG+AGG 0.650129 7:+52966928 None:intergenic
TAGTGCAGAAGATGATCTGG+AGG 0.658411 7:-52967580 MsaT037172.1:intron
AAGGCTACAGTTGAAATCTG+AGG 0.661211 7:-52967702 MsaT037172.1:CDS
AAGGTTGCGAAATGTTTATG+CGG 0.662196 7:-52965178 MsaT037172.1:CDS
ATATATCTAAAGACTCCAAG+TGG 0.664356 7:-52975387 MsaT037172.1:CDS
CATATGTAGGGACCAAGAAG+GGG 0.668859 7:-52966240 MsaT037172.1:CDS
GCTACAGTTGAAATCTGAGG+CGG 0.677312 7:-52967699 MsaT037172.1:CDS
GTGGATAAGGGTTAATGACG+AGG 0.682278 7:-52968009 MsaT037172.1:CDS
TTACACGGCGAGTTGGAGAG+GGG 0.686320 7:-52965242 MsaT037172.1:CDS
AGATAATTAAGACATTGCGA+AGG 0.692374 7:+52964741 None:intergenic
TAATATACTCACAAGCATCG+CGG 0.704453 7:+52967979 None:intergenic
ATGACGAAGATTGCTACAGG+AGG 0.705189 7:-52964638 MsaT037172.1:intron
CACCATTAGAGAGAGATTTG+GGG 0.718287 7:-52966735 MsaT037172.1:CDS
GGCTAGCACTAACCTCCTAG+AGG 0.719203 7:-52973182 MsaT037172.1:CDS
ATCACACGGGAAATTGTCCG+AGG 0.719984 7:-52965426 MsaT037172.1:CDS
AACACAACTGAAGAATTACG+AGG 0.731861 7:-52967948 MsaT037172.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 52964458 52975665 52964458 ID=MsaG037172
Chr7 mRNA 52964458 52975665 52964458 ID=MsaT037172.1;Parent=MsaG037172
Chr7 exon 52964458 52964557 52964458 ID=MsaT037172.1.exon19;Parent=MsaT037172.1
Chr7 CDS 52964458 52964557 52964458 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52964639 52964853 52964639 ID=MsaT037172.1.exon18;Parent=MsaT037172.1
Chr7 CDS 52964639 52964853 52964639 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52964968 52965073 52964968 ID=MsaT037172.1.exon17;Parent=MsaT037172.1
Chr7 CDS 52964968 52965073 52964968 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52965152 52965276 52965152 ID=MsaT037172.1.exon16;Parent=MsaT037172.1
Chr7 CDS 52965152 52965276 52965152 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52965355 52965519 52965355 ID=MsaT037172.1.exon15;Parent=MsaT037172.1
Chr7 CDS 52965355 52965519 52965355 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52965609 52965713 52965609 ID=MsaT037172.1.exon14;Parent=MsaT037172.1
Chr7 CDS 52965609 52965713 52965609 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52965924 52966000 52965924 ID=MsaT037172.1.exon13;Parent=MsaT037172.1
Chr7 CDS 52965924 52966000 52965924 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52966204 52966291 52966204 ID=MsaT037172.1.exon12;Parent=MsaT037172.1
Chr7 CDS 52966204 52966291 52966204 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52966715 52966783 52966715 ID=MsaT037172.1.exon11;Parent=MsaT037172.1
Chr7 CDS 52966715 52966783 52966715 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52966889 52967002 52966889 ID=MsaT037172.1.exon10;Parent=MsaT037172.1
Chr7 CDS 52966889 52967002 52966889 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52967194 52967301 52967194 ID=MsaT037172.1.exon9;Parent=MsaT037172.1
Chr7 CDS 52967194 52967301 52967194 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52967530 52967594 52967530 ID=MsaT037172.1.exon8;Parent=MsaT037172.1
Chr7 CDS 52967530 52967594 52967530 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52967672 52967854 52967672 ID=MsaT037172.1.exon7;Parent=MsaT037172.1
Chr7 CDS 52967672 52967854 52967672 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52967949 52968341 52967949 ID=MsaT037172.1.exon6;Parent=MsaT037172.1
Chr7 CDS 52967949 52968341 52967949 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52973173 52973207 52973173 ID=MsaT037172.1.exon5;Parent=MsaT037172.1
Chr7 CDS 52973173 52973207 52973173 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52973874 52973974 52973874 ID=MsaT037172.1.exon4;Parent=MsaT037172.1
Chr7 CDS 52973874 52973974 52973874 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52974680 52974801 52974680 ID=MsaT037172.1.exon3;Parent=MsaT037172.1
Chr7 CDS 52974680 52974801 52974680 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52974992 52975460 52974992 ID=MsaT037172.1.exon2;Parent=MsaT037172.1
Chr7 CDS 52974992 52975460 52974992 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Chr7 exon 52975594 52975665 52975594 ID=MsaT037172.1.exon1;Parent=MsaT037172.1
Chr7 CDS 52975594 52975665 52975594 ID=cds.MsaT037172.1;Parent=MsaT037172.1
Gene Sequence

>MsaT037172.1

ATGGCTAATGAATTTCCTCCATCCCATTTTTTCAAGATCATATGTGATCAAAGCCTTCATCAAGGAAAGCTTATGATCCCAAGAAAGTTTGTGGAGAAATATGGAGAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAGTGGTGCAAAGTGGAGATTAGATTTGGTAAAAAGTGATGGAAAAATATGGTTTCAAAAGGGTTGGAAACAATTTGTGAAGTATCATTCTTTATCTCAAGGCCATCTTCTGCTTTTCAAATATGGAAGAACTTCCCATTTTGAAGTGCACATCTTCGATAAGAGTGCCTTAGAGATAAAATACCCTTTCCAAAGAGTTGAAGCCAAAAGGGTATCCAACGATCTTGGCAATAAACCTCCGAATGATGAAGATTTGGAAGATTATATAGCAAGTAAAAAGAGAAAAACTAACTTGTCATTAGAATTTAATCAACCATGTGAAATTGGAAGCAATAGCTGTGTTAAAGTTGGGAAATTGGAAAAGGTAGCAGCTTTGAATCATATTGACAAAAAGTGTAAAGGAAAGCAATTGGCTGTTGCTAAGAAAGTTACAACCTTTGACAGAGCAAACTCTATGAGAACTTATAATCCATCTTTTATTGCTGTCGTGCGTCCATCATACATTGATGCTGCTTGTAATCTGAACATACCAAGTGAGTTTGGTAAAAGACACTTATTAGGTTTAGGCAAAAAGCAAGGAGATATTCATCTTAGGTTATTAAATGGGAGAGTTTGGCCTGCAAGGGAGGCTAGCACTAACCTCCTAGAGGCTTTTCAAGAGAGACTAACAATTGAAAGGGAAAAATTGTGGAATTCTAGAGAGGATATCATTGATGAGCAACAACAACGTTTCGAATTGGAGATTGAGAAGGCTAGGTCGACCTCACAATTGAATTTGATTCGTCATCTTTTGCAGTCAATGCCAGTTCAACCAGAACTAGAACTAGAAGAAGAAACATACGGTCCACTTCTCAAGTATATAATTGACATGGGAGATGTTGGACAATTTCAAATTTTCTCTAAATTGATTAAAGATCATATTCACAATTCAATATCAATTTCGCGATTGGGTTATTATGACATGTTGATGTGGATAAGGGTTAATGACGAGGCCATGATCCGCGATGCTTGTGAGTATATTAAAAGGGAAAACACAACTGAAGAATTACGAGAAAACTACTTTTTGGCTCTTTGTGAAAGTGATGACCGCAAGGCACAGATTTCAGATGTGCTTAAAAATACAGACATTACAGAGTTTACTTCCACAGATGAAATATTTTCAAATATATTCAAATTATTAGGAAGGCTACAGTTGAAATCTGAGGCGGAAAAGTTACTATTCGAATGTTATGACGAAGATGATCTGGAGGAAATTTCAAACTTCATTGATATTTATGCATTTAGCATTCCAAATTTAGAGGTTGAGCATATCATAAAAGAGATTGAGAATTTGCATGGTCTGTTGAAAATTTCACCTACATTCTCTTTGTATGAAAAGCTAATTTTGTATTGCTGTGGTAAGAACAAGGTGGATGTAGCTATTTATCTTGTTGATAAAATGTGTGAAGCAGGCTTTATGCCCTCCAGTCATGTAATGCAATCTGTATTAGAGACTTGCTCAGAAAATAATCAAGATTTTCAGACTAGACGGGATATGATTACGAATGACACCATTAGAGAGAGATTTGGGGTAGCACCTATAGTAAAAAAGAAAAGGCGCCCGGTGCACAAATGCTCCAGCATATGTAGGGACCAAGAAGGGGTCACGCCATTTGGTGTATTGTATACAGCCTTAACTTCATTCTTTCTAATTTGTCTTGTTTTACTAATATCTGGCTGTTCATTTTCTATTCCTTTTGCTATATTGACAATCAAGTATTATGAAGAGATGGAACAATCTGGAATCAACTACGCAAAAGAAGTTTTATTAGACCAAATTCATGCATATGCAGATTACGATGAATTTGAGAAAGCAAAACAGGTTGTTCAAGATCAACGCATACCAGTGAAGTGGTTAATTGAAATTAAAAGAATGCTTGTCTCATCTCTTGCATCACACGGGAAATTGTCCGAGGCACTTGTTTTGTTGGAGGAAATCAAGAAAGCTGGACAAACTTTGAACCCTAGAGCTGTTTTATGTCTCATGGATGTGTTATGTTTACACGGCGAGTTGGAGAGGGGTCTTCTCCTACGCACGGAGTTGAGTGGCGAAGATTGGGTTCAAGGTTGCGAAATGTTTATGCGGTACTCGGTTCAATACAAAAATTTAAGTTCTACCATTGAAATGTTTAAACAACTCAAGGATCATTTCGAAAGTGATGCATTCAAGAGAGAGAATCTTTTTGGAGGAAGTAGTATGTATAGTCTTTGTGTTGAAGCCTTTGAGCTAATTTTTGAATATGGGTCCACACATTTGCAATTTGGATTGGACTTGCTGGATCTAATAAAAAAAGAGGTTGGCCGTGTTCCTTCGCAATGTCTTAATTATCTCCTATCTGCTTGTGCCAAATCAAGAGATTTGAATAAAGCTAATTTGGTTTGGAAAGAATATGCATTGGCTGGATATTATTATGACGAAGATTGCTACAGGAGAATGTATCACGTACTTTTGGCCTCGGGAGAACACAAATCTGCTGACAGTATACTCGTTAATAAAATTCCCAGTTTTTACTCTGGTCTTTGTTCGATCTGA
Protein sequence

>MsaT037172.1

MANEFPPSHFFKIICDQSLHQGKLMIPRKFVEKYGEGLSNAIYLKTPSGAKWRLDLVKSDGKIWFQKGWKQFVKYHSLSQGHLLLFKYGRTSHFEVHIFDKSALEIKYPFQRVEAKRVSNDLGNKPPNDEDLEDYIASKKRKTNLSLEFNQPCEIGSNSCVKVGKLEKVAALNHIDKKCKGKQLAVAKKVTTFDRANSMRTYNPSFIAVVRPSYIDAACNLNIPSEFGKRHLLGLGKKQGDIHLRLLNGRVWPAREASTNLLEAFQERLTIEREKLWNSREDIIDEQQQRFELEIEKARSTSQLNLIRHLLQSMPVQPELELEEETYGPLLKYIIDMGDVGQFQIFSKLIKDHIHNSISISRLGYYDMLMWIRVNDEAMIRDACEYIKRENTTEELRENYFLALCESDDRKAQISDVLKNTDITEFTSTDEIFSNIFKLLGRLQLKSEAEKLLFECYDEDDLEEISNFIDIYAFSIPNLEVEHIIKEIENLHGLLKISPTFSLYEKLILYCCGKNKVDVAIYLVDKMCEAGFMPSSHVMQSVLETCSENNQDFQTRRDMITNDTIRERFGVAPIVKKKRRPVHKCSSICRDQEGVTPFGVLYTALTSFFLICLVLLISGCSFSIPFAILTIKYYEEMEQSGINYAKEVLLDQIHAYADYDEFEKAKQVVQDQRIPVKWLIEIKRMLVSSLASHGKLSEALVLLEEIKKAGQTLNPRAVLCLMDVLCLHGELERGLLLRTELSGEDWVQGCEMFMRYSVQYKNLSSTIEMFKQLKDHFESDAFKRENLFGGSSMYSLCVEAFELIFEYGSTHLQFGLDLLDLIKKEVGRVPSQCLNYLLSACAKSRDLNKANLVWKEYALAGYYYDEDCYRRMYHVLLASGEHKSADSILVNKIPSFYSGLCSI*