AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037285

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037285 MtrunA17_Chr7g0234641 97.462 197 5 0 10 206 83 279 2.62e-141 401
MsaG037285 MtrunA17_Chr8g0338741 62.121 198 72 3 10 206 93 288 1.06e-82 252
MsaG037285 MtrunA17_Chr4g0032761 39.806 206 113 4 12 206 87 292 2.51e-53 176
MsaG037285 MtrunA17_Chr2g0329011 43.434 198 91 3 10 206 79 256 1.06e-50 169
MsaG037285 MtrunA17_Chr2g0309421 43.961 207 99 4 14 206 244 447 2.98e-49 169
MsaG037285 MtrunA17_Chr1g0179101 44.878 205 100 3 14 206 539 742 8.88e-48 167
MsaG037285 MtrunA17_Chr7g0250631 42.439 205 104 4 14 206 433 635 1.20e-47 166
MsaG037285 MtrunA17_Chr2g0290691 40.284 211 107 4 14 206 273 482 1.89e-47 164
MsaG037285 MtrunA17_Chr7g0266031 44.878 205 100 4 14 206 561 764 1.12e-46 164
MsaG037285 MtrunA17_Chr8g0335351 39.806 206 109 3 14 206 439 642 8.39e-45 159
MsaG037285 MtrunA17_Chr2g0285711 40.722 194 108 4 14 206 156 343 9.98e-45 154
MsaG037285 MtrunA17_Chr2g0321101 38.462 208 111 4 12 206 159 362 1.88e-44 154
MsaG037285 MtrunA17_Chr7g0227331 40.952 210 107 5 14 206 301 510 6.49e-44 155
MsaG037285 MtrunA17_Chr4g0063311 41.872 203 96 4 22 206 173 371 8.47e-44 153
MsaG037285 MtrunA17_Chr3g0080101 39.806 206 107 3 16 206 486 689 1.85e-43 155
MsaG037285 MtrunA17_Chr8g0381091 39.024 205 98 3 14 206 152 341 2.55e-43 151
MsaG037285 MtrunA17_Chr4g0030361 38.579 197 80 3 12 206 374 531 7.09e-43 152
MsaG037285 MtrunA17_Chr8g0369091 40.722 194 81 4 13 206 665 824 9.32e-43 153
MsaG037285 MtrunA17_Chr2g0312641 41.530 183 89 3 24 206 173 337 3.23e-42 148
MsaG037285 MtrunA17_Chr8g0356291 39.437 213 100 4 14 206 196 399 1.75e-41 147
MsaG037285 MtrunA17_Chr6g0449401 34.583 240 110 4 14 206 332 571 9.03e-41 147
MsaG037285 MtrunA17_Chr2g0327561 37.681 207 103 3 12 218 693 873 3.18e-39 143
MsaG037285 MtrunA17_Chr6g0478601 35.938 192 91 3 13 204 177 336 1.36e-30 117
MsaG037285 MtrunA17_Chr5g0428981 35.385 195 93 4 10 204 826 987 2.27e-28 112
MsaG037285 MtrunA17_Chr4g0018201 34.359 195 95 3 10 204 822 983 3.14e-28 112
MsaG037285 MtrunA17_Chr7g0261491 30.208 192 111 4 13 204 186 354 1.43e-24 100
MsaG037285 MtrunA17_Chr1g0183651 29.688 192 112 4 13 204 186 354 3.98e-24 99.8
MsaG037285 MtrunA17_Chr2g0299781 29.703 202 108 5 13 204 179 356 5.36e-24 99.4
MsaG037285 MtrunA17_Chr3g0135951 29.703 202 108 6 13 204 160 337 5.62e-24 99.0
MsaG037285 MtrunA17_Chr4g0059751 30.412 194 67 5 13 204 162 289 1.58e-23 97.4
MsaG037285 MtrunA17_Chr1g0153321 32.474 194 106 6 13 204 174 344 4.08e-23 96.7
MsaG037285 MtrunA17_Chr1g0167351 29.688 192 114 4 13 204 176 346 1.76e-22 95.1
MsaG037285 MtrunA17_Chr1g0167311 29.167 192 115 4 13 204 181 351 2.54e-22 94.4
MsaG037285 MtrunA17_Chr7g0245031 29.167 192 72 3 13 204 214 341 2.81e-22 94.0
MsaG037285 MtrunA17_Chr1g0187581 29.688 192 113 4 13 204 183 352 3.43e-22 94.0
MsaG037285 MtrunA17_Chr1g0197721 30.570 193 81 5 14 206 118 257 4.18e-21 90.9
MsaG037285 MtrunA17_Chr2g0286501 26.500 200 88 6 6 204 102 243 9.21e-16 75.9
MsaG037285 MtrunA17_Chr3g0116701 27.273 176 89 3 29 204 176 312 2.92e-13 68.2
MsaG037285 MtrunA17_Chr4g0058241 28.249 177 86 5 29 204 180 316 4.91e-12 64.3
MsaG037285 MtrunA17_Chr5g0407121 30.366 191 110 8 29 204 125 307 2.69e-11 62.4
MsaG037285 MtrunA17_Chr1g0188031 38.272 81 48 1 14 94 89 167 3.52e-11 62.0
MsaG037285 MtrunA17_Chr3g0107051 35.484 93 57 1 13 105 87 176 4.71e-11 61.6
MsaG037285 MtrunA17_Chr3g0125381 27.273 143 97 3 12 149 17 157 5.39e-11 60.1
MsaG037285 MtrunA17_Chr1g0150321 37.079 89 53 1 6 94 72 157 5.76e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037285 AT4G13000.1 57.360 197 67 4 12 206 86 267 4.07e-68 213
MsaG037285 AT3G25250.1 58.376 197 69 6 12 206 89 274 1.53e-66 211
MsaG037285 AT3G20830.1 42.929 198 103 4 12 206 92 282 1.62e-50 169
MsaG037285 AT3G12690.1 42.661 218 94 4 14 214 252 455 4.61e-49 169
MsaG037285 AT3G12690.3 42.661 218 94 4 14 214 252 455 4.61e-49 169
MsaG037285 AT3G12690.2 42.661 218 94 4 14 214 252 455 4.61e-49 169
MsaG037285 AT5G03640.2 43.379 219 88 6 14 206 608 816 1.00e-48 171
MsaG037285 AT5G03640.1 43.379 219 88 6 14 206 608 816 1.00e-48 171
MsaG037285 AT5G40030.1 44.976 209 92 6 14 206 181 382 2.01e-48 166
MsaG037285 AT2G36350.1 43.602 211 101 3 14 206 626 836 2.84e-48 169
MsaG037285 AT3G52890.2 43.458 214 99 4 14 206 605 817 3.34e-48 169
MsaG037285 AT3G52890.4 43.458 214 99 4 14 206 605 817 3.34e-48 169
MsaG037285 AT3G52890.1 43.458 214 99 4 14 206 605 817 3.34e-48 169
MsaG037285 AT3G52890.3 43.458 214 99 4 14 206 605 817 3.34e-48 169
MsaG037285 AT1G51170.1 41.538 195 102 4 15 206 96 281 5.72e-48 162
MsaG037285 AT2G34650.1 43.434 198 100 4 14 210 147 333 5.85e-48 163
MsaG037285 AT5G47750.1 40.952 210 105 4 14 206 258 465 2.53e-47 164
MsaG037285 AT1G79250.1 41.589 214 112 4 14 214 213 426 8.10e-46 159
MsaG037285 AT1G79250.3 41.589 214 112 4 14 214 213 426 8.10e-46 159
MsaG037285 AT1G79250.2 41.589 214 112 4 14 214 213 426 8.10e-46 159
MsaG037285 AT1G79250.4 41.589 214 112 4 14 214 213 426 8.10e-46 159
MsaG037285 AT4G26610.1 37.864 206 114 2 14 206 190 394 8.36e-46 159
MsaG037285 AT5G55910.2 37.799 209 114 2 14 206 176 384 1.38e-45 158
MsaG037285 AT5G55910.1 37.799 209 114 2 14 206 176 384 1.38e-45 158
MsaG037285 AT1G16440.1 44.615 195 104 3 14 206 182 374 2.62e-45 157
MsaG037285 AT5G58140.6 40.000 195 83 3 12 206 642 802 2.02e-44 158
MsaG037285 AT5G58140.7 40.000 195 83 3 12 206 642 802 2.02e-44 158
MsaG037285 AT5G58140.5 40.000 195 83 3 12 206 642 802 2.02e-44 158
MsaG037285 AT5G58140.2 40.000 195 83 3 12 206 642 802 2.02e-44 158
MsaG037285 AT5G58140.1 40.000 195 83 3 12 206 642 802 2.02e-44 158
MsaG037285 AT5G58140.3 40.000 195 83 3 12 206 642 802 2.36e-44 158
MsaG037285 AT3G44610.1 38.462 208 115 5 12 206 144 351 1.11e-43 152
MsaG037285 AT2G44830.2 40.000 210 105 4 14 206 430 635 6.87e-43 154
MsaG037285 AT2G44830.3 40.000 210 105 4 14 206 430 635 6.87e-43 154
MsaG037285 AT2G44830.1 40.000 210 105 4 14 206 430 635 6.87e-43 154
MsaG037285 AT3G27580.2 39.713 209 107 4 14 206 249 454 4.45e-42 150
MsaG037285 AT3G27580.1 39.713 209 107 4 14 206 249 454 4.45e-42 150
MsaG037285 AT1G53700.1 41.848 184 89 3 24 206 171 337 8.59e-41 145
MsaG037285 AT2G26700.1 34.413 247 106 5 12 206 154 396 9.63e-41 145
MsaG037285 AT3G14370.1 39.459 185 94 4 24 206 165 333 1.35e-38 139
MsaG037285 AT3G45780.2 35.885 209 98 2 12 218 728 902 2.84e-38 141
MsaG037285 AT3G45780.1 35.885 209 98 2 12 218 728 902 2.84e-38 141
MsaG037285 AT5G62310.1 34.896 192 93 3 13 204 820 979 1.22e-29 116
MsaG037285 AT3G17850.1 35.519 183 86 2 22 204 957 1107 8.86e-29 114
MsaG037285 AT1G45160.2 35.897 195 83 3 13 204 736 891 1.67e-28 113
MsaG037285 AT1G45160.1 35.897 195 83 3 13 204 736 891 1.74e-28 112
MsaG037285 AT1G48490.4 34.135 208 90 6 4 204 881 1048 7.49e-27 108
MsaG037285 AT1G48490.3 34.135 208 90 6 4 204 880 1047 8.25e-27 108
MsaG037285 AT1G48490.2 34.135 208 90 6 4 204 880 1047 8.25e-27 108
MsaG037285 AT1G48490.1 34.135 208 90 6 4 204 880 1047 8.25e-27 108
MsaG037285 AT4G33080.1 29.703 202 108 6 13 204 160 337 6.34e-25 102
MsaG037285 AT4G33080.2 29.703 202 108 6 13 204 160 337 7.35e-25 102
MsaG037285 AT1G30640.3 31.250 192 114 4 13 204 93 266 9.01e-25 101
MsaG037285 AT1G30640.2 31.250 192 114 4 13 204 186 359 1.19e-24 101
MsaG037285 AT1G30640.1 31.250 192 114 4 13 204 186 359 1.19e-24 101
MsaG037285 AT2G19400.1 29.353 201 110 4 13 204 171 348 1.50e-24 101
MsaG037285 AT2G20470.3 28.500 200 103 4 13 204 190 357 2.33e-24 100
MsaG037285 AT2G20470.2 28.500 200 103 4 13 204 190 357 2.95e-24 100
MsaG037285 AT2G20470.1 28.500 200 103 4 13 204 190 357 2.95e-24 100
MsaG037285 AT5G09890.2 32.292 192 111 5 13 204 168 340 2.32e-23 97.8
MsaG037285 AT5G09890.1 32.292 192 111 5 13 204 168 340 2.36e-23 97.8
MsaG037285 AT3G23310.1 27.094 203 106 3 13 204 186 357 2.67e-22 94.7
MsaG037285 AT3G10540.1 30.928 194 81 5 13 206 111 251 3.94e-22 94.4
MsaG037285 AT3G08730.1 29.231 195 74 4 10 204 197 327 5.46e-22 93.6
MsaG037285 AT3G08730.2 29.231 195 74 4 10 204 197 327 5.46e-22 93.6
MsaG037285 AT3G08720.1 29.231 195 74 4 10 204 203 333 5.55e-22 93.6
MsaG037285 AT3G08720.3 29.231 195 74 4 10 204 203 333 5.55e-22 93.6
MsaG037285 AT3G08720.4 29.231 195 74 4 10 204 203 333 5.55e-22 93.6
MsaG037285 AT3G08720.2 29.231 195 74 4 10 204 203 333 5.55e-22 93.6
MsaG037285 AT4G14350.2 27.638 199 108 4 13 204 185 354 9.72e-22 93.2
MsaG037285 AT4G14350.1 27.638 199 108 4 13 204 185 354 9.72e-22 93.2
MsaG037285 AT4G14350.4 27.638 199 108 4 13 204 185 354 9.72e-22 93.2
MsaG037285 AT4G14350.3 27.638 199 108 4 13 204 185 354 1.05e-21 93.2
MsaG037285 AT5G04510.3 30.570 193 81 5 14 206 111 250 1.10e-21 91.3
MsaG037285 AT1G03920.1 28.500 200 104 4 13 204 203 371 1.34e-21 92.8
MsaG037285 AT1G03920.3 28.500 200 104 4 13 204 203 371 1.34e-21 92.8
MsaG037285 AT1G03920.2 28.500 200 104 4 13 204 90 258 1.72e-21 92.0
MsaG037285 AT5G04510.1 30.412 194 82 5 13 206 110 250 2.40e-21 92.0
MsaG037285 AT5G04510.2 30.570 193 81 5 14 206 111 250 2.81e-21 91.3
MsaG037285 AT5G18700.1 41.584 101 55 2 6 104 57 155 7.56e-18 82.4
MsaG037285 AT2G25090.1 31.633 98 65 1 3 100 72 167 5.37e-11 62.0
MsaG037285 AT1G54960.1 32.955 88 57 1 10 97 136 221 5.96e-11 62.0
MsaG037285 AT1G54960.2 32.955 88 57 1 10 97 136 221 5.96e-11 62.0
MsaG037285 AT1G09000.2 32.955 88 57 1 10 97 137 222 9.85e-11 61.2

Find 38 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATGAGTTTGGTGATTT+TGG 0.015954 7:+55259569 None:intergenic
TTTCAAACTGAAAAGCTTAT+TGG 0.148224 7:-55260441 MsaT037285.1:CDS
CCTCTCTTGCCTAGGTTTAA+AGG 0.151835 7:-55260468 MsaT037285.1:intron
TGAAATGTTTCCTTCCTATT+TGG 0.201550 7:+55259234 None:intergenic
TTAGGGAATGGACATAATTT+TGG 0.205600 7:-55259323 MsaT037285.1:CDS
GTGGCACCTGAAGTTATATT+AGG 0.227326 7:-55259341 MsaT037285.1:CDS
TGACTCAATGATTCAAATTT+CGG 0.238923 7:+55259446 None:intergenic
TTGGAGTATCTACACAATTT+AGG 0.242514 7:-55259686 MsaT037285.1:CDS
ATATCAACTCGGTTTGTAAA+AGG 0.283970 7:-55259418 MsaT037285.1:CDS
TTGGATTAAGTTTCTTTGAT+AGG 0.376257 7:+55259588 None:intergenic
TGGCACCTGAAGTTATATTA+GGG 0.404506 7:-55259340 MsaT037285.1:CDS
ATTCTTGTAATTCTTATGTT+GGG 0.407852 7:-55259472 MsaT037285.1:CDS
GGTGTTGATTGGTGGTCATT+TGG 0.413313 7:-55259302 MsaT037285.1:CDS
ACGCGTTTAGTAGTCCAAAT+AGG 0.421731 7:-55259248 MsaT037285.1:CDS
AAATATCATGATTCAACAAA+CGG 0.426253 7:-55259636 MsaT037285.1:CDS
TATTCTTGTAATTCTTATGT+TGG 0.434882 7:-55259473 MsaT037285.1:CDS
CCATTCCCTAATATAACTTC+AGG 0.445354 7:+55259335 None:intergenic
TTTGCTATTGATTATTGTCA+TGG 0.483556 7:-55260417 MsaT037285.1:CDS
GAAAGAATGGTGAATGCAAA+AGG 0.496992 7:-55260530 MsaT037285.1:CDS
AATATCATGATTCAACAAAC+GGG 0.499507 7:-55259635 MsaT037285.1:CDS
TTGAGTTCCAACTCGTCACC+AGG 0.507046 7:-55259551 MsaT037285.1:CDS
GAGTTGGAACTCAATGAGTT+TGG 0.516698 7:+55259560 None:intergenic
AATTCTCTGAAGAAACCATC+AGG 0.520550 7:-55260353 MsaT037285.1:intron
TTTCGTATTTGGTGGTGAGT+TGG 0.528298 7:+55259526 None:intergenic
TGAAAAGTCAAACTCGTTCG+TGG 0.546520 7:-55259378 MsaT037285.1:CDS
TTTGGTGGTGAGTTGGAACC+TGG 0.553367 7:+55259533 None:intergenic
CCTGAAGTTATATTAGGGAA+TGG 0.555674 7:-55259335 MsaT037285.1:CDS
CTATTGATTATTGTCATGGT+GGG 0.561164 7:-55260413 MsaT037285.1:CDS
AGTTTATAGAGATTTGAAGC+CGG 0.590893 7:-55259660 MsaT037285.1:CDS
GTTTAGTAGTCCAAATAGGA+AGG 0.600674 7:-55259244 MsaT037285.1:CDS
GAAAAGTCAAACTCGTTCGT+GGG 0.611855 7:-55259377 MsaT037285.1:CDS
AGTGGAATTGGTGTTAGCAT+TGG 0.614201 7:-55259705 MsaT037285.1:CDS
GTTGGAACCTGGTGACGAGT+TGG 0.630791 7:+55259544 None:intergenic
GAGTCAAATTGATATCAACT+CGG 0.631076 7:-55259429 MsaT037285.1:CDS
CGTGGGAACTGAGGATTACG+TGG 0.637947 7:-55259360 MsaT037285.1:CDS
AAAGAATGGTGAATGCAAAA+GGG 0.638261 7:-55260529 MsaT037285.1:intron
GCTATTGATTATTGTCATGG+TGG 0.639438 7:-55260414 MsaT037285.1:CDS
AAACTCGTTCGTGGGAACTG+AGG 0.645067 7:-55259369 MsaT037285.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 55259223 55260555 55259223 ID=MsaG037285
Chr7 mRNA 55259223 55260555 55259223 ID=MsaT037285.1;Parent=MsaG037285
Chr7 exon 55259223 55259736 55259223 ID=MsaT037285.1.exon3;Parent=MsaT037285.1
Chr7 CDS 55259223 55259736 55259223 ID=cds.MsaT037285.1;Parent=MsaT037285.1
Chr7 exon 55260354 55260476 55260354 ID=MsaT037285.1.exon2;Parent=MsaT037285.1
Chr7 CDS 55260354 55260476 55260354 ID=cds.MsaT037285.1;Parent=MsaT037285.1
Chr7 exon 55260530 55260555 55260530 ID=MsaT037285.1.exon1;Parent=MsaT037285.1
Chr7 CDS 55260530 55260555 55260530 ID=cds.MsaT037285.1;Parent=MsaT037285.1
Gene Sequence

>MsaT037285.1

ATGGAAAGAATGGTGAATGCAAAAGGGTTTAAAGGAGCTTTTCAAACTGAAAAGCTTATTGGATTTGCTATTGATTATTGTCATGGTGGGAATTTGCATTCTCTTAGAAAGAAACAGCCTGAGAAAAAATTCTCTGAAGAAACCATCAGGTTTTATGCAGTGGAATTGGTGTTAGCATTGGAGTATCTACACAATTTAGGAGTAGTTTATAGAGATTTGAAGCCGGAAAATATCATGATTCAACAAACGGGTCACATAATGATCGTAGATTTCGACCTATCAAAGAAACTTAATCCAAAATCACCAAACTCATTGAGTTCCAACTCGTCACCAGGTTCCAACTCACCACCAAATACGAAAAACAGAAGGAAGAGATGGTTATCAAAGTTCTATTCTTGTAATTCTTATGTTGGGAAACCGAAATTTGAATCATTGAGTCAAATTGATATCAACTCGGTTTGTAAAAGGAGTGAGTCAGACTCAGTTGAAAAGTCAAACTCGTTCGTGGGAACTGAGGATTACGTGGCACCTGAAGTTATATTAGGGAATGGACATAATTTTGGTGTTGATTGGTGGTCATTTGGCGTCGTTTTGTATGAGTTGTTTTATGGAGCGACGCGTTTAGTAGTCCAAATAGGAAGGAAACATTTCACAAGATTTTAA
Protein sequence

>MsaT037285.1

MERMVNAKGFKGAFQTEKLIGFAIDYCHGGNLHSLRKKQPEKKFSEETIRFYAVELVLALEYLHNLGVVYRDLKPENIMIQQTGHIMIVDFDLSKKLNPKSPNSLSSNSSPGSNSPPNTKNRRKRWLSKFYSCNSYVGKPKFESLSQIDINSVCKRSESDSVEKSNSFVGTEDYVAPEVILGNGHNFGVDWWSFGVVLYELFYGATRLVVQIGRKHFTRF*