AlfalfaGEDB | MsaG037286

M. sativa ssp. caerulea / MsaG037286

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG037286	AES77546.1	91.200	125	11	0	1	125	1	125	1.50e-72	223

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG037286	sp\|Q9FJK3\|AGL80_ARATH	51.613	124	59	1	1	123	1	124	1.54e-29	111

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG037286	tr\|G7L356\|G7L356_MEDTR	91.200	125	11	0	1	125	1	125	7.17e-73	223

TFs/TRs:

Gene ID	Type	Classification
MsaG037286	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG037286	MtrunA17_Chr7g0218151	91.200	125	11	0	1	125	1	125	1.38e-76	223
MsaG037286	MtrunA17_Chr4g0012761	91.200	125	11	0	1	125	1	125	9.30e-75	219
MsaG037286	MtrunA17_Chr1g0188661	84.000	125	20	0	1	125	1	125	5.23e-69	204
MsaG037286	MtrunA17_Chr5g0420351	76.000	125	30	0	1	125	1	125	7.47e-65	194
MsaG037286	MtrunA17_Chr1g0193311	78.400	125	27	0	1	125	1	125	1.34e-60	183
MsaG037286	MtrunA17_Chr1g0188681	69.600	125	38	0	1	125	1	125	1.09e-58	178
MsaG037286	MtrunA17_Chr1g0188651	77.869	122	27	0	1	122	1	122	2.09e-56	172
MsaG037286	MtrunA17_Chr1g0197261	57.600	125	53	0	1	125	1	125	1.57e-49	155
MsaG037286	MtrunA17_Chr1g0197461	58.537	123	51	0	3	125	2	124	9.56e-48	150
MsaG037286	MtrunA17_Chr1g0197321	54.472	123	56	0	1	123	1	123	3.54e-47	149
MsaG037286	MtrunA17_Chr4g0014321	45.455	121	65	1	1	120	1	121	4.93e-37	125
MsaG037286	MtrunA17_Chr3g0110131	52.381	126	59	1	1	125	1	126	1.01e-33	117
MsaG037286	MtrunA17_Chr3g0107381	49.153	118	59	1	1	117	1	118	1.57e-33	116
MsaG037286	MtrunA17_Chr4g0013041	42.857	126	69	2	1	125	1	124	3.59e-33	115
MsaG037286	MtrunA17_Chr3g0107331	46.552	116	61	1	3	117	2	117	2.13e-30	107
MsaG037286	MtrunA17_Chr3g0107351	46.667	120	63	1	3	121	2	121	5.13e-30	105
MsaG037286	MtrunA17_Chr8g0351751	43.750	128	69	1	1	125	1	128	5.19e-30	108
MsaG037286	MtrunA17_Chr4g0014031	47.009	117	61	1	1	116	1	117	4.34e-28	102
MsaG037286	MtrunA17_Chr3g0107361	44.828	116	63	1	3	117	2	117	2.00e-27	99.4
MsaG037286	MtrunA17_Chr3g0107341	43.966	116	64	1	3	117	2	117	4.00e-27	98.6
MsaG037286	MtrunA17_Chr2g0296831	41.525	118	68	1	1	117	1	118	8.99e-26	94.0
MsaG037286	MtrunA17_Chr2g0283941	41.026	117	68	1	1	116	1	117	4.19e-24	92.8
MsaG037286	MtrunA17_Chr3g0107461	36.508	126	79	1	1	125	1	126	4.35e-24	90.9
MsaG037286	MtrunA17_Chr5g0432691	36.066	122	77	1	1	121	1	122	8.07e-24	90.1
MsaG037286	MtrunA17_Chr4g0014131	46.154	117	62	1	1	116	1	117	1.53e-23	91.3
MsaG037286	MtrunA17_Chr4g0014121	42.735	117	66	1	1	116	29	145	1.51e-21	84.7
MsaG037286	MtrunA17_Chr4g0008701	36.000	125	72	3	1	121	1	121	7.49e-18	78.2
MsaG037286	MtrunA17_Chr2g0296861	37.234	94	58	1	1	93	1	94	1.52e-17	73.9
MsaG037286	MtrunA17_Chr7g0267601	35.398	113	69	2	1	109	1	113	4.48e-17	75.9
MsaG037286	MtrunA17_Chr4g0031421	37.234	94	58	1	29	121	1	94	1.00e-15	68.6
MsaG037286	MtrunA17_Chr3g0105791	38.298	94	57	1	29	121	1	94	1.48e-15	68.2
MsaG037286	MtrunA17_Chr3g0106501	38.710	93	56	1	29	120	1	93	6.15e-14	64.3
MsaG037286	MtrunA17_Chr3g0128721	32.292	96	63	1	3	98	6	99	3.68e-12	62.0
MsaG037286	MtrunA17_Chr6g0449861	36.842	95	53	4	1	88	9	103	6.60e-12	61.2
MsaG037286	MtrunA17_Chr6g0457851	32.500	80	48	3	1	74	11	90	2.20e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG037286	AT5G48670.1	51.613	124	59	1	1	123	1	124	1.57e-30	111
MsaG037286	AT3G05860.2	42.975	121	68	1	1	120	1	121	2.94e-30	108
MsaG037286	AT3G05860.3	44.068	118	65	1	1	117	1	118	9.17e-30	107
MsaG037286	AT3G05860.1	44.068	118	65	1	1	117	1	118	1.28e-29	107
MsaG037286	AT1G65330.1	40.496	121	71	1	3	122	2	122	1.83e-26	100
MsaG037286	AT1G65300.1	38.017	121	74	1	3	122	2	122	2.41e-26	99.8
MsaG037286	AT5G26630.1	45.763	118	63	1	1	117	1	118	6.57e-25	94.7
MsaG037286	AT5G26650.1	40.870	115	67	1	4	117	2	116	1.83e-23	93.6
MsaG037286	AT5G27960.1	40.870	115	67	1	4	117	2	116	2.76e-23	92.4
MsaG037286	AT2G28700.1	36.290	124	78	1	1	123	1	124	8.98e-22	88.6
MsaG037286	AT5G27810.1	43.750	96	53	1	29	123	1	96	2.85e-21	82.8
MsaG037286	AT1G31630.1	34.483	116	74	2	3	117	2	116	1.64e-20	85.1
MsaG037286	AT1G31640.1	37.931	116	70	2	3	117	2	116	2.56e-20	85.5
MsaG037286	AT1G22590.2	33.607	122	68	2	1	115	1	116	8.53e-20	80.1
MsaG037286	AT5G26580.1	37.607	117	60	1	1	117	1	104	1.80e-17	77.0
MsaG037286	AT2G40210.1	37.815	119	69	2	1	116	1	117	1.48e-15	72.0
MsaG037286	AT5G06500.1	34.921	126	78	3	1	124	1	124	1.24e-13	65.5
MsaG037286	AT5G58890.1	35.417	96	55	2	1	89	1	96	2.78e-13	65.1

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTTCTTGTTGAATCAT+TGG	0.233760	7:+55263023	None:intergenic
TGTTGAATCATTGGAAATAA+AGG	0.251603	7:+55263032	None:intergenic
TTTGCAAAAGGAAACTTTCT+TGG	0.282244	7:+55262806	None:intergenic
ACCTTTCCTCCTCCTCTTGT+AGG	0.282788	7:+55262999	None:intergenic
AGTTGAACATTCTTATGTTT+TGG	0.291264	7:-55262726	MsaT037286.1:CDS
TGTTTGGCTTCCTTTAGATT+TGG	0.353502	7:+55262883	None:intergenic
ATACCTCTCAATCACCTGTT+TGG	0.354870	7:+55262867	None:intergenic
TTCCAATGATTCAACAAGAA+AGG	0.389550	7:-55263025	MsaT037286.1:CDS
AAATCTAAAGGAAGCCAAAC+AGG	0.425294	7:-55262881	MsaT037286.1:CDS
CTTTAGTAATCATTTGCAAA+AGG	0.447168	7:+55262794	None:intergenic
TGCAAATGATTACTAAAGCT+AGG	0.481563	7:-55262789	MsaT037286.1:CDS
TTTCACCTTCTTCCTAGTCA+TGG	0.490416	7:+55263059	None:intergenic
GCAAATGATTACTAAAGCTA+GGG	0.492435	7:-55262788	MsaT037286.1:CDS
ACATGCAAAACAACAAGCTT+CGG	0.496923	7:-55262702	MsaT037286.1:CDS
ACCTCTGTTTCAGAATCAAA+AGG	0.516061	7:+55262910	None:intergenic
ACCTACAAGAGGAGGAGGAA+AGG	0.522508	7:-55263000	MsaT037286.1:CDS
AGAGGTGTGGCCAAATCTAA+AGG	0.522555	7:-55262893	MsaT037286.1:CDS
AGAGAACTCACTATTCTTTG+TGG	0.526508	7:-55262961	MsaT037286.1:CDS
CAAAGCCATGACTAGGAAGA+AGG	0.543154	7:-55263064	None:intergenic
AGGAGACCTACAAGAGGAGG+AGG	0.545154	7:-55263005	MsaT037286.1:CDS
GAAAGGAGACCTACAAGAGG+AGG	0.592612	7:-55263008	MsaT037286.1:CDS
TACATTGCAAAGCCATGACT+AGG	0.609749	7:-55263071	None:intergenic
GAGGAAAGGTATCATCAAGA+AGG	0.620547	7:-55262986	MsaT037286.1:CDS
TTGATTCTGAAACAGAGGTG+TGG	0.630350	7:-55262906	MsaT037286.1:CDS
AAGCCAAACAGGTGATTGAG+AGG	0.637552	7:-55262870	MsaT037286.1:CDS
CAAGAAAGGAGACCTACAAG+AGG	0.685284	7:-55263011	MsaT037286.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	55262693	55263079	55262693	ID=MsaG037286
Chr7	mRNA	55262693	55263079	55262693	ID=MsaT037286.1;Parent=MsaG037286
Chr7	exon	55262693	55263079	55262693	ID=MsaT037286.1.exon1;Parent=MsaT037286.1
Chr7	CDS	55262693	55263079	55262693	ID=cds.MsaT037286.1;Parent=MsaT037286.1

Gene Sequence

>MsaT037286.1

ATGACTAGGAAGAAGGTGAAACTCGCCTTTATTTCCAATGATTCAACAAGAAAGGAGACCTACAAGAGGAGGAGGAAAGGTATCATCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTTCAGCATGTGCTATAATTTCTGATCCTTTTGATTCTGAAACAGAGGTGTGGCCAAATCTAAAGGAAGCCAAACAGGTGATTGAGAGGTATCAAAACTCATATATGAAAGATGAGAGAAAAAATGTGAACCAAGAAAGTTTCCTTTTGCAAATGATTACTAAAGCTAGGGAACAACTGAGAATGCAAAGACACGACAATCATGAGAATGAGTTGAACATTCTTATGTTTTGGTACATGCAAAACAACAAGCTTCGGATGATGTGA

Protein sequence

>MsaT037286.1

MTRKKVKLAFISNDSTRKETYKRRRKGIIKKVRELTILCGISACAIISDPFDSETEVWPNLKEAKQVIERYQNSYMKDERKNVNQESFLLQMITKAREQLRMQRHDNHENELNILMFWYMQNNKLRMM*