AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037297


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037297 MtrunA17_Chr1g0188661 91.195 159 14 0 1 159 1 159 1.35e-97 278
MsaG037297 MtrunA17_Chr7g0218151 88.050 159 19 0 1 159 1 159 3.01e-94 270
MsaG037297 MtrunA17_Chr4g0012761 86.164 159 22 0 1 159 1 159 4.09e-94 269
MsaG037297 MtrunA17_Chr1g0188681 71.338 157 45 0 1 157 1 157 1.07e-78 230
MsaG037297 MtrunA17_Chr1g0188651 74.214 159 39 1 1 159 1 157 4.82e-75 221
MsaG037297 MtrunA17_Chr5g0420351 67.949 156 50 0 1 156 1 156 4.75e-73 216
MsaG037297 MtrunA17_Chr1g0193311 72.436 156 43 0 1 156 1 156 6.31e-73 216
MsaG037297 MtrunA17_Chr1g0197261 56.774 155 67 0 1 155 1 155 4.68e-58 178
MsaG037297 MtrunA17_Chr1g0197321 55.128 156 69 1 1 155 1 156 5.15e-56 173
MsaG037297 MtrunA17_Chr1g0197461 54.248 153 70 0 3 155 2 154 1.09e-54 169
MsaG037297 MtrunA17_Chr3g0110131 49.045 157 79 1 1 156 1 157 1.07e-38 131
MsaG037297 MtrunA17_Chr5g0432691 39.103 156 94 1 1 155 1 156 3.42e-37 125
MsaG037297 MtrunA17_Chr3g0107381 42.484 153 87 1 1 152 1 153 5.51e-36 124
MsaG037297 MtrunA17_Chr4g0014321 36.774 155 96 2 1 153 1 155 3.09e-34 119
MsaG037297 MtrunA17_Chr3g0107331 41.401 157 91 1 3 158 2 158 2.81e-32 112
MsaG037297 MtrunA17_Chr4g0014031 42.667 150 84 2 1 148 1 150 6.51e-32 114
MsaG037297 MtrunA17_Chr3g0107361 40.909 154 90 1 3 155 2 155 8.45e-31 108
MsaG037297 MtrunA17_Chr3g0107351 41.935 155 87 2 3 155 2 155 3.45e-30 107
MsaG037297 MtrunA17_Chr4g0014121 42.500 160 90 2 1 158 29 188 5.45e-30 107
MsaG037297 MtrunA17_Chr4g0013041 35.484 155 96 3 1 153 1 153 8.78e-30 107
MsaG037297 MtrunA17_Chr3g0107341 39.610 154 92 1 3 155 2 155 2.27e-29 105
MsaG037297 MtrunA17_Chr2g0283941 38.994 159 95 2 1 157 1 159 9.49e-29 105
MsaG037297 MtrunA17_Chr8g0351751 36.478 159 97 2 1 155 1 159 5.29e-28 104
MsaG037297 MtrunA17_Chr4g0014131 39.752 161 95 2 1 159 1 161 6.39e-27 100
MsaG037297 MtrunA17_Chr3g0107461 32.704 159 105 2 1 157 1 159 8.16e-26 96.3
MsaG037297 MtrunA17_Chr2g0296831 41.026 117 68 1 1 116 1 117 1.57e-24 92.0
MsaG037297 MtrunA17_Chr2g0296861 32.258 155 104 1 1 154 1 155 2.48e-21 84.7
MsaG037297 MtrunA17_Chr3g0105791 34.884 129 82 2 31 157 3 131 5.97e-21 83.2
MsaG037297 MtrunA17_Chr4g0031421 34.884 129 82 2 31 157 3 131 1.41e-20 82.4
MsaG037297 MtrunA17_Chr3g0106501 35.156 128 81 2 32 157 4 131 1.81e-19 79.7
MsaG037297 MtrunA17_Chr4g0008701 33.553 152 94 3 1 148 1 149 1.97e-18 81.3
MsaG037297 MtrunA17_Chr7g0267601 37.168 113 67 2 1 109 1 113 3.67e-17 77.4
MsaG037297 MtrunA17_Chr3g0106421 33.594 128 81 3 31 155 3 129 2.07e-16 73.2
MsaG037297 MtrunA17_Chr3g0128721 27.632 152 105 2 3 151 6 155 4.57e-13 65.9
MsaG037297 MtrunA17_Chr1g0161931 40.426 94 55 1 64 156 28 121 1.03e-11 58.5
MsaG037297 MtrunA17_Chr1g0162261 37.500 96 59 1 64 158 22 117 1.12e-11 58.5
MsaG037297 MtrunA17_Chr6g0449861 29.464 112 73 3 1 106 9 120 3.91e-11 60.1
MsaG037297 MtrunA17_Chr1g0162181 38.542 96 58 1 64 158 22 117 5.01e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037297 AT5G48670.1 45.513 156 84 1 1 155 1 156 7.48e-34 121
MsaG037297 AT3G05860.2 38.926 149 90 1 1 148 1 149 3.79e-30 108
MsaG037297 AT5G26630.1 38.750 160 97 1 1 159 1 160 1.17e-29 108
MsaG037297 AT3G05860.3 38.926 149 90 1 1 148 1 149 2.05e-29 108
MsaG037297 AT3G05860.1 38.926 149 90 1 1 148 1 149 2.65e-29 108
MsaG037297 AT1G65300.1 36.054 147 93 1 3 148 2 148 6.26e-27 102
MsaG037297 AT1G65330.1 36.735 147 92 1 3 148 2 148 2.10e-26 101
MsaG037297 AT5G26650.1 35.294 153 98 1 4 155 2 154 6.76e-24 95.9
MsaG037297 AT5G27960.1 43.925 107 59 1 4 109 2 108 2.73e-23 93.6
MsaG037297 AT1G22590.2 29.012 162 99 3 1 155 1 153 1.93e-20 82.8
MsaG037297 AT5G27810.1 45.238 84 45 1 31 113 3 86 1.59e-19 79.3
MsaG037297 AT1G31630.1 29.032 155 108 2 3 156 2 155 3.05e-19 82.8
MsaG037297 AT2G28700.1 33.813 139 89 2 1 138 1 137 5.76e-18 79.3
MsaG037297 AT1G31640.1 38.739 111 66 2 3 112 2 111 1.28e-17 79.0
MsaG037297 AT5G26580.1 36.697 109 56 1 1 109 1 96 1.48e-15 72.8
MsaG037297 AT2G40210.1 37.864 103 59 2 1 100 1 101 1.59e-12 64.3
MsaG037297 AT5G58890.1 33.333 111 66 3 1 104 1 110 3.21e-12 63.2
MsaG037297 AT5G06500.1 35.849 106 64 2 1 102 1 106 1.36e-11 60.8

Find 26 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCAAAAGGAAACTTTCT+TGG 0.253590 7:+55453599 None:intergenic
AACAGAGGTGTGGCCAAATC+TGG 0.333286 7:-55453689 MsaT037297.1:CDS
TGTTGAATCATTGGAGATAA+AGG 0.341938 7:+55453825 None:intergenic
CTTGTCTTTGCTTTCTCAGT+TGG 0.347479 7:+55453557 None:intergenic
AGCCTTTCTTGTTGAATCAT+TGG 0.353862 7:+55453816 None:intergenic
CTCCAATGATTCAACAAGAA+AGG 0.407412 7:-55453818 MsaT037297.1:CDS
AAATCTGGAGGAAGCAAAAC+AGG 0.418438 7:-55453674 MsaT037297.1:CDS
CTTTAGTAATCTGTTGCAAA+AGG 0.444882 7:+55453587 None:intergenic
TCTTTCTGATTTGAAAGACT+TGG 0.461416 7:-55453461 MsaT037297.1:CDS
TTTCACCTTCTTTCTAGTCA+TGG 0.476937 7:+55453852 None:intergenic
TCAGAAAGAGTGATATCATC+TGG 0.482942 7:+55453475 None:intergenic
GAAGAAAAGTATCATAATGA+AGG 0.494513 7:-55453779 MsaT037297.1:CDS
CAGAACTCATATATGAAAGA+TGG 0.506115 7:-55453637 MsaT037297.1:CDS
AGCTGAACATTCGTATGAGC+TGG 0.525005 7:-55453519 MsaT037297.1:CDS
AGAGAACTCACTATTCTTTG+TGG 0.526508 7:-55453754 MsaT037297.1:CDS
TGCAACAGATTACTAAAGCT+AGG 0.531119 7:-55453582 MsaT037297.1:CDS
GCAACAGATTACTAAAGCTA+GGG 0.532673 7:-55453581 MsaT037297.1:CDS
GAAGGAAATTGATGACAAGA+TGG 0.550366 7:-55453416 MsaT037297.1:CDS
AGGATCGGAAATTATAGCAC+AGG 0.559456 7:+55453723 None:intergenic
CGAAGCCATGACTAGAAAGA+AGG 0.562075 7:-55453857 None:intergenic
GAAAGGCTTCCTACAAGAAG+AGG 0.563850 7:-55453801 MsaT037297.1:CDS
TCGGAAATTATAGCACAGGC+TGG 0.570119 7:+55453727 None:intergenic
TTGATTCTAAAACAGAGGTG+TGG 0.633348 7:-55453699 MsaT037297.1:CDS
AAGCAAAACAGGTGATTGAG+AGG 0.635256 7:-55453663 MsaT037297.1:CDS
AGAGGTGTGGCCAAATCTGG+AGG 0.647190 7:-55453686 MsaT037297.1:CDS
CTGGTACCTGCAAAACAACA+CGG 0.672567 7:-55453500 MsaT037297.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 55453393 55453872 55453393 ID=MsaG037297
Chr7 mRNA 55453393 55453872 55453393 ID=MsaT037297.1;Parent=MsaG037297
Chr7 exon 55453393 55453872 55453393 ID=MsaT037297.1.exon1;Parent=MsaT037297.1
Chr7 CDS 55453393 55453872 55453393 ID=cds.MsaT037297.1;Parent=MsaT037297.1
Gene Sequence

>MsaT037297.1

ATGACTAGAAAGAAGGTGAAACTCGCCTTTATCTCCAATGATTCAACAAGAAAGGCTTCCTACAAGAAGAGGAAGAAAAGTATCATAATGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTCCAGCCTGTGCTATAATTTCCGATCCTTTTGATTCTAAAACAGAGGTGTGGCCAAATCTGGAGGAAGCAAAACAGGTGATTGAGAGGTATCAGAACTCATATATGAAAGATGGCAGAAAAAATGTGGACCAAGAAAGTTTCCTTTTGCAACAGATTACTAAAGCTAGGGACCAACTGAGAAAGCAAAGACAAGACAATCATGAAAATGAGCTGAACATTCGTATGAGCTGGTACCTGCAAAACAACACGGTGCCAGATGATATCACTCTTTCTGATTTGAAAGACTTGGATAAGTTAATTGCAAAAAGTATGAAGGAAATTGATGACAAGATGGCTTCACTTAGTTTATCAAACTGA

Protein sequence

>MsaT037297.1

MTRKKVKLAFISNDSTRKASYKKRKKSIIMKVRELTILCGIPACAIISDPFDSKTEVWPNLEEAKQVIERYQNSYMKDGRKNVDQESFLLQQITKARDQLRKQRQDNHENELNIRMSWYLQNNTVPDDITLSDLKDLDKLIAKSMKEIDDKMASLSLSN*