AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037298

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037298 MtrunA17_Chr1g0188661 92.453 159 12 0 1 159 1 159 2.21e-98 280
MsaG037298 MtrunA17_Chr4g0012761 87.421 159 20 0 1 159 1 159 3.83e-95 272
MsaG037298 MtrunA17_Chr7g0218151 89.308 159 17 0 1 159 1 159 3.91e-95 272
MsaG037298 MtrunA17_Chr1g0188681 71.338 157 45 0 1 157 1 157 9.67e-79 230
MsaG037298 MtrunA17_Chr1g0188651 75.472 159 37 1 1 159 1 157 6.78e-76 223
MsaG037298 MtrunA17_Chr5g0420351 69.231 156 48 0 1 156 1 156 1.07e-74 220
MsaG037298 MtrunA17_Chr1g0193311 73.718 156 41 0 1 156 1 156 1.23e-73 217
MsaG037298 MtrunA17_Chr1g0197261 57.419 155 66 0 1 155 1 155 5.31e-59 180
MsaG037298 MtrunA17_Chr1g0197321 55.769 156 68 1 1 155 1 156 4.03e-57 176
MsaG037298 MtrunA17_Chr1g0197461 55.556 153 68 0 3 155 2 154 3.44e-56 173
MsaG037298 MtrunA17_Chr3g0110131 50.318 157 77 1 1 156 1 157 1.70e-39 133
MsaG037298 MtrunA17_Chr3g0107381 43.791 153 85 1 1 152 1 153 1.58e-37 128
MsaG037298 MtrunA17_Chr5g0432691 39.103 156 94 1 1 155 1 156 1.67e-37 126
MsaG037298 MtrunA17_Chr4g0014321 38.065 155 94 2 1 153 1 155 6.74e-36 124
MsaG037298 MtrunA17_Chr3g0107331 42.675 157 89 1 3 158 2 158 8.20e-34 116
MsaG037298 MtrunA17_Chr4g0014031 44.000 150 82 2 1 148 1 150 1.03e-32 115
MsaG037298 MtrunA17_Chr3g0107361 42.208 154 88 1 3 155 2 155 2.12e-32 113
MsaG037298 MtrunA17_Chr3g0107351 43.226 155 85 2 3 155 2 155 9.79e-32 111
MsaG037298 MtrunA17_Chr4g0013041 36.774 155 94 3 1 153 1 153 2.05e-31 112
MsaG037298 MtrunA17_Chr3g0107341 40.909 154 90 1 3 155 2 155 5.25e-31 109
MsaG037298 MtrunA17_Chr8g0351751 37.736 159 95 2 1 155 1 159 9.08e-30 108
MsaG037298 MtrunA17_Chr4g0014121 42.500 160 90 2 1 158 29 188 2.17e-29 106
MsaG037298 MtrunA17_Chr2g0283941 38.994 159 95 2 1 157 1 159 1.03e-28 105
MsaG037298 MtrunA17_Chr4g0014131 40.994 161 93 2 1 159 1 161 9.56e-28 103
MsaG037298 MtrunA17_Chr3g0107461 32.298 161 107 2 1 159 1 161 6.16e-26 96.7
MsaG037298 MtrunA17_Chr2g0296831 42.735 117 66 1 1 116 1 117 2.67e-25 94.0
MsaG037298 MtrunA17_Chr2g0296861 32.258 155 104 1 1 154 1 155 1.20e-21 85.5
MsaG037298 MtrunA17_Chr3g0105791 34.351 131 84 2 29 157 1 131 7.97e-21 82.8
MsaG037298 MtrunA17_Chr4g0031421 34.351 131 84 2 29 157 1 131 1.38e-20 82.4
MsaG037298 MtrunA17_Chr3g0106501 34.351 131 84 2 29 157 1 131 3.32e-19 79.0
MsaG037298 MtrunA17_Chr4g0008701 32.895 152 95 3 1 148 1 149 1.13e-17 79.0
MsaG037298 MtrunA17_Chr3g0106421 33.077 130 83 3 29 155 1 129 2.21e-16 73.2
MsaG037298 MtrunA17_Chr7g0267601 35.185 108 69 1 3 109 6 113 2.24e-16 75.1
MsaG037298 MtrunA17_Chr1g0161931 41.489 94 54 1 64 156 28 121 2.37e-12 60.5
MsaG037298 MtrunA17_Chr3g0128721 26.974 152 106 2 3 151 6 155 2.78e-12 63.5
MsaG037298 MtrunA17_Chr1g0162261 38.542 96 58 1 64 158 22 117 2.84e-12 60.1
MsaG037298 MtrunA17_Chr6g0449861 30.357 112 72 3 1 106 9 120 8.89e-12 61.6
MsaG037298 MtrunA17_Chr1g0162181 39.583 96 57 1 64 158 22 117 1.40e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037298 AT5G48670.1 45.513 156 84 1 1 155 1 156 3.91e-33 119
MsaG037298 AT3G05860.2 39.597 149 89 1 1 148 1 149 4.04e-31 111
MsaG037298 AT3G05860.3 39.597 149 89 1 1 148 1 149 2.13e-30 110
MsaG037298 AT5G26630.1 40.000 160 95 1 1 159 1 160 2.69e-30 109
MsaG037298 AT3G05860.1 39.597 149 89 1 1 148 1 149 2.89e-30 110
MsaG037298 AT1G65300.1 36.054 147 93 1 3 148 2 148 3.85e-27 103
MsaG037298 AT1G65330.1 36.735 147 92 1 3 148 2 148 1.14e-26 101
MsaG037298 AT5G26650.1 35.294 153 98 1 4 155 2 154 4.82e-24 96.3
MsaG037298 AT5G27960.1 43.925 107 59 1 4 109 2 108 2.00e-23 94.0
MsaG037298 AT1G22590.2 29.630 162 98 3 1 155 1 153 6.47e-21 84.0
MsaG037298 AT1G31630.1 29.032 155 108 2 3 156 2 155 1.11e-19 84.3
MsaG037298 AT5G27810.1 44.186 86 47 1 29 113 1 86 1.37e-19 79.3
MsaG037298 AT2G28700.1 35.252 139 87 2 1 138 1 137 2.01e-19 83.2
MsaG037298 AT1G31640.1 39.640 111 65 2 3 112 2 111 1.42e-18 82.0
MsaG037298 AT5G26580.1 38.532 109 54 1 1 109 1 96 3.56e-17 77.4
MsaG037298 AT5G58890.1 35.135 111 64 3 1 104 1 110 1.21e-13 67.0
MsaG037298 AT2G40210.1 38.835 103 58 2 1 100 1 101 1.56e-13 67.4
MsaG037298 AT5G06500.1 35.849 106 64 2 1 102 1 106 7.41e-12 61.6

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCAAAAGGAAACTTTCC+TGG 0.331570 7:+55476765 None:intergenic
AACAGAGGTGTGGCCAAATC+TGG 0.333286 7:-55476855 MsaT037298.1:CDS
TGTTGAATCATTGGAGATAA+AGG 0.341938 7:+55476991 None:intergenic
CTTGTCTTTGCTTTCTCAGT+TGG 0.347479 7:+55476723 None:intergenic
AGCCTTTCTTGTTGAATCAT+TGG 0.353862 7:+55476982 None:intergenic
CTCCAATGATTCAACAAGAA+AGG 0.407412 7:-55476984 MsaT037298.1:CDS
AAATCTGGAGGAAGCAAAAC+AGG 0.418438 7:-55476840 MsaT037298.1:CDS
CTTTAGTAATCTGTTGCAAA+AGG 0.444882 7:+55476753 None:intergenic
CAGAACTCATATATGAAAGA+TGG 0.456034 7:-55476803 MsaT037298.1:CDS
TCTTTCTGATTTGAAAGACT+TGG 0.461416 7:-55476627 MsaT037298.1:CDS
TCAGAAAGAGTGATATCATC+TGG 0.482942 7:+55476641 None:intergenic
TTTCACCTTCTTCCTAGTCA+TGG 0.490416 7:+55477018 None:intergenic
AGCTGAACATTCGTATGAGC+TGG 0.525005 7:-55476685 MsaT037298.1:CDS
AGAGAACTCACTATTCTTTG+TGG 0.526508 7:-55476920 MsaT037298.1:CDS
TGCAACAGATTACTAAAGCT+AGG 0.531119 7:-55476748 MsaT037298.1:CDS
GCAACAGATTACTAAAGCTA+GGG 0.532673 7:-55476747 MsaT037298.1:CDS
AGAACTCATATATGAAAGAT+GGG 0.538835 7:-55476802 MsaT037298.1:CDS
GAAGGAAATTGATGACAAGA+TGG 0.550366 7:-55476582 MsaT037298.1:CDS
CGAAGCCATGACTAGGAAGA+AGG 0.551367 7:-55477023 None:intergenic
GAAGAAAAGTATCATCAAGA+AGG 0.556586 7:-55476945 MsaT037298.1:CDS
AGGATCGGAAATTATAGCAC+AGG 0.559456 7:+55476889 None:intergenic
GAAAGGCTTCCTACAAGAAG+AGG 0.563850 7:-55476967 MsaT037298.1:CDS
TCGGAAATTATAGCACAGGC+TGG 0.570119 7:+55476893 None:intergenic
TACATTGCGAAGCCATGACT+AGG 0.618148 7:-55477030 None:intergenic
TTGATTCTAAAACAGAGGTG+TGG 0.633348 7:-55476865 MsaT037298.1:CDS
AAGCAAAACAGGTGATTGAG+AGG 0.635256 7:-55476829 MsaT037298.1:CDS
AGAGGTGTGGCCAAATCTGG+AGG 0.647190 7:-55476852 MsaT037298.1:CDS
CTGGTACCTGCAAAACAACA+CGG 0.672567 7:-55476666 MsaT037298.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 55476559 55477038 55476559 ID=MsaG037298
Chr7 mRNA 55476559 55477038 55476559 ID=MsaT037298.1;Parent=MsaG037298
Chr7 exon 55476559 55477038 55476559 ID=MsaT037298.1.exon1;Parent=MsaT037298.1
Chr7 CDS 55476559 55477038 55476559 ID=cds.MsaT037298.1;Parent=MsaT037298.1
Gene Sequence

>MsaT037298.1

ATGACTAGGAAGAAGGTGAAACTCGCCTTTATCTCCAATGATTCAACAAGAAAGGCTTCCTACAAGAAGAGGAAGAAAAGTATCATCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTCCAGCCTGTGCTATAATTTCCGATCCTTTTGATTCTAAAACAGAGGTGTGGCCAAATCTGGAGGAAGCAAAACAGGTGATTGAGAGGTATCAGAACTCATATATGAAAGATGGGAGAAAAAATGTGAACCAGGAAAGTTTCCTTTTGCAACAGATTACTAAAGCTAGGGACCAACTGAGAAAGCAAAGACAAGACAATCATGAAAATGAGCTGAACATTCGTATGAGCTGGTACCTGCAAAACAACACGGTGCCAGATGATATCACTCTTTCTGATTTGAAAGACTTGGATAAGTTAATTGCAAAAAGTATGAAGGAAATTGATGACAAGATGGCTTCACTTAGTTTATCAAACTGA
Protein sequence

>MsaT037298.1

MTRKKVKLAFISNDSTRKASYKKRKKSIIKKVRELTILCGIPACAIISDPFDSKTEVWPNLEEAKQVIERYQNSYMKDGRKNVNQESFLLQQITKARDQLRKQRQDNHENELNIRMSWYLQNNTVPDDITLSDLKDLDKLIAKSMKEIDDKMASLSLSN*