AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037458

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037458 MtrunA17_Chr7g0259571 86.925 413 48 2 314 725 1 408 0.0 707
MsaG037458 MtrunA17_Chr2g0334311 64.570 477 158 3 256 722 840 1315 0.0 599
MsaG037458 MtrunA17_Chr2g0287291 59.454 476 181 3 257 722 845 1318 1.04e-172 530
MsaG037458 MtrunA17_Chr7g0245401 42.117 444 238 6 280 718 879 1308 3.94e-100 335
MsaG037458 MtrunA17_Chr3g0092621 79.452 73 15 0 56 128 84 156 2.34e-32 123
MsaG037458 MtrunA17_Chr6g0488471 73.611 72 19 0 49 120 90 161 6.79e-32 125
MsaG037458 MtrunA17_Chr5g0415191 69.892 93 28 0 41 133 87 179 2.05e-31 125
MsaG037458 MtrunA17_Chr4g0055931 70.667 75 22 0 49 123 88 162 2.40e-31 125
MsaG037458 MtrunA17_Chr2g0331031 69.863 73 22 0 51 123 104 176 1.43e-30 123
MsaG037458 MtrunA17_Chr8g0389241 70.270 74 21 1 47 120 96 168 3.04e-30 120
MsaG037458 MtrunA17_Chr4g0059701 91.892 74 6 0 51 124 79 152 7.58e-30 119
MsaG037458 MtrunA17_Chr1g0210471 64.935 77 27 0 46 122 114 190 1.41e-28 114
MsaG037458 MtrunA17_Chr4g0062741 65.753 73 25 0 50 122 132 204 3.73e-28 115
MsaG037458 MtrunA17_Chr1g0162081 65.753 73 25 0 50 122 116 188 1.06e-27 114
MsaG037458 MtrunA17_Chr3g0104071 65.753 73 25 0 50 122 141 213 1.40e-26 111
MsaG037458 MtrunA17_Chr5g0429521 65.753 73 25 0 50 122 126 198 2.16e-26 110
MsaG037458 MtrunA17_Chr7g0241761 60.976 82 28 1 44 121 124 205 4.66e-26 109
MsaG037458 MtrunA17_Chr5g0436501 56.471 85 35 1 42 124 111 195 2.48e-24 103
MsaG037458 MtrunA17_Chr6g0452791 75.000 60 15 0 56 115 80 139 5.49e-24 99.4
MsaG037458 MtrunA17_Chr3g0135671 61.972 71 24 1 53 120 88 158 6.06e-23 98.2
MsaG037458 MtrunA17_Chr5g0409841 42.708 96 49 2 34 123 66 161 1.35e-18 85.5
MsaG037458 MtrunA17_Chr5g0440731 45.238 84 40 2 52 129 101 184 1.35e-17 84.0
MsaG037458 MtrunA17_Chr5g0409831 56.667 60 22 1 48 103 65 124 1.41e-16 79.7
MsaG037458 MtrunA17_Chr5g0440741 47.436 78 35 2 58 129 102 179 2.13e-16 80.5
MsaG037458 MtrunA17_Chr8g0365011 39.062 128 57 4 58 171 94 214 1.45e-15 77.8
MsaG037458 MtrunA17_Chr8g0364731 59.322 59 22 1 65 121 10 68 1.31e-13 70.1
MsaG037458 MtrunA17_Chr1g0192061 50.000 70 31 2 58 124 63 131 1.39e-13 69.7
MsaG037458 MtrunA17_Chr5g0432921 49.254 67 31 2 58 122 115 180 2.65e-12 67.0
MsaG037458 MtrunA17_Chr5g0432941 47.761 67 32 2 58 122 115 180 1.03e-11 65.5
MsaG037458 MtrunA17_Chr3g0113121 47.222 72 31 2 58 122 97 168 1.64e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037458 AT2G48110.1 57.231 484 188 6 252 721 793 1271 1.42e-170 523
MsaG037458 AT3G23590.1 55.782 441 192 3 280 719 867 1305 3.49e-155 484
MsaG037458 AT5G47390.1 59.375 96 39 0 28 123 68 163 4.47e-32 128
MsaG037458 AT1G70000.1 73.256 86 23 0 41 126 83 168 6.54e-30 119
MsaG037458 AT1G70000.2 73.256 86 23 0 41 126 83 168 6.54e-30 119
MsaG037458 AT5G04760.1 66.667 75 25 0 48 122 91 165 1.05e-29 117
MsaG037458 AT1G49010.1 70.667 75 21 1 49 122 126 200 6.04e-29 118
MsaG037458 AT1G74840.2 74.419 86 22 0 43 128 83 168 2.39e-27 111
MsaG037458 AT1G74840.1 74.419 86 22 0 43 128 83 168 2.61e-27 112
MsaG037458 AT2G38090.1 66.667 72 24 0 51 122 136 207 2.64e-27 113
MsaG037458 AT1G19000.2 68.889 90 28 0 41 130 87 176 3.46e-27 112
MsaG037458 AT1G19000.1 68.889 90 28 0 41 130 87 176 3.46e-27 112
MsaG037458 AT5G08520.1 50.943 106 46 1 17 122 85 184 3.72e-27 112
MsaG037458 AT5G56840.1 66.667 72 23 1 52 122 87 158 4.02e-27 110
MsaG037458 AT5G58900.1 66.667 72 24 0 51 122 136 207 8.36e-27 111
MsaG037458 AT5G61620.1 64.384 73 26 0 49 121 101 173 1.15e-26 111
MsaG037458 AT3G16350.1 69.737 76 23 0 51 126 132 207 8.30e-26 110
MsaG037458 AT5G01200.1 61.644 73 28 0 50 122 140 212 1.86e-24 103
MsaG037458 AT3G11280.2 54.348 92 41 1 47 138 118 208 6.13e-24 102
MsaG037458 AT3G11280.1 54.348 92 41 1 47 138 118 208 6.13e-24 102
MsaG037458 AT5G05790.2 56.627 83 32 1 44 122 115 197 5.70e-23 100
MsaG037458 AT5G05790.1 56.627 83 32 1 44 122 115 197 5.70e-23 100
MsaG037458 AT4G09450.1 51.765 85 37 1 41 121 72 156 4.65e-20 89.4
MsaG037458 AT3G10580.2 46.809 94 48 1 28 121 67 158 1.21e-19 89.7
MsaG037458 AT3G10580.1 46.809 94 48 1 28 121 67 158 1.84e-19 89.7
MsaG037458 AT5G23650.1 55.556 72 32 0 50 121 114 185 4.99e-19 89.7
MsaG037458 AT3G10590.1 51.471 68 32 1 57 124 112 178 7.57e-16 77.4

Find 172 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCACTGATATTATGCTTT+TGG 0.214303 7:-58519316 MsaT037458.1:CDS
GCTTCATTGGTTTGGATATC+AGG 0.221476 7:-58517357 MsaT037458.1:CDS
CTCGCAATGGCGAGCTCAAC+GGG 0.232491 7:+58520950 None:intergenic
CACGCCAACGGTTCACCTTT+TGG 0.252464 7:+58517796 None:intergenic
GGCCTTGCTTCCCAAGTTGC+TGG 0.276296 7:-58517624 MsaT037458.1:CDS
GGTGGTGTTGGTGCCCTTCT+TGG 0.278240 7:-58517651 MsaT037458.1:CDS
CTTAGTCTCTTCAACAATTC+CGG 0.282016 7:+58516982 None:intergenic
CACTCGGTGCTTCATTGGTT+TGG 0.290629 7:-58517365 MsaT037458.1:CDS
TAGGAGACACTGTTCCTTCC+TGG 0.305385 7:-58517308 MsaT037458.1:CDS
TGTTTCTTTACATTGTCTTT+AGG 0.312553 7:+58517468 None:intergenic
CAACTAAATAAAATCAAGTA+TGG 0.329967 7:-58517447 MsaT037458.1:CDS
GGTTATGGGTAGCAGAGGTT+TGG 0.337295 7:-58525387 MsaT037458.1:CDS
CAAGTTTGTATGTTATTGAT+AGG 0.337377 7:+58517922 None:intergenic
TGAGTTGGCTCTTCGCCTTT+TGG 0.338379 7:-58516932 MsaT037458.1:CDS
GAAGCTTGATGCTTTGGTTA+TGG 0.340692 7:-58525402 MsaT037458.1:CDS
AAGCTTGATGCTTTGGTTAT+GGG 0.345992 7:-58525401 MsaT037458.1:CDS
ATAACCTTTGCTCGTCGATT+TGG 0.348498 7:+58517147 None:intergenic
GTTATGGGTAGCAGAGGTTT+GGG 0.355817 7:-58525386 MsaT037458.1:CDS
AAAGGTTATTGCAATTCATT+TGG 0.357195 7:-58517133 MsaT037458.1:CDS
GCGATTGCGGCGATGGCAAC+AGG 0.362165 7:+58521050 None:intergenic
AGATGATTGTTGATTCAGTT+GGG 0.362498 7:-58525430 MsaT037458.1:CDS
GGGATCTCTCCAGTTGCTCC+AGG 0.364712 7:-58517600 MsaT037458.1:CDS
CGCGACACTCGGTGCTTCAT+TGG 0.368434 7:-58517370 MsaT037458.1:CDS
TAGAGAATCCCTGGAGCAAC+TGG 0.376437 7:+58517591 None:intergenic
TTAAGAGTGTACCGGTCTAT+TGG 0.380018 7:-58517567 MsaT037458.1:CDS
GAACAGGATGATGGAGAATC+TGG 0.385401 7:-58517264 MsaT037458.1:CDS
GAGATGATTGTTGATTCAGT+TGG 0.386103 7:-58525431 MsaT037458.1:CDS
GGATTTGAAGCTTGATGCTT+TGG 0.387071 7:-58525408 MsaT037458.1:CDS
CAAAGATGCTGGAATAAAAT+CGG 0.409250 7:+58518406 None:intergenic
ATCAGCTGATACGTTAGAAC+AGG 0.412475 7:-58517280 MsaT037458.1:CDS
CCGTTGCTTCTGTTTGCCCC+TGG 0.415514 7:-58517851 MsaT037458.1:CDS
CTTGAGATGTGTTGCGAGCT+CGG 0.418088 7:-58521078 MsaT037458.1:intron
GATATCGCCGTCTCCATTAC+TGG 0.420969 7:+58519373 None:intergenic
TAACCTCCTGATTGATTGAC+TGG 0.422854 7:+58519226 None:intergenic
AAGTTGGTTCAATCATTGAT+AGG 0.430117 7:-58517327 MsaT037458.1:CDS
ACGCTTCGCGATTGCGGCGA+TGG 0.430565 7:+58521043 None:intergenic
ACACAATTTCGCACAACATC+AGG 0.434894 7:+58520890 None:intergenic
AGCTCATTAGTACATATTGT+TGG 0.436090 7:-58522486 MsaT037458.1:intron
AAGCAACGGCATTGAGAGTT+GGG 0.438037 7:+58517865 None:intergenic
GTTGGTGCCCTTCTTGGTCA+TGG 0.438526 7:-58517645 MsaT037458.1:CDS
ACTTGGCAAGCCTATGTGTC+AGG 0.440784 7:-58517057 MsaT037458.1:CDS
TCTACATGTATGAATAATAA+TGG 0.444873 7:-58517672 MsaT037458.1:CDS
GGTAAATATGCAGCAGGGCT+AGG 0.450158 7:+58518305 None:intergenic
ACCTTTGCTCGTCGATTTGG+TGG 0.453322 7:+58517150 None:intergenic
CTTTCATGAATGGAACTGAT+TGG 0.454720 7:-58518328 MsaT037458.1:CDS
CTTGCTTCCCAAGTTGCTGG+TGG 0.458658 7:-58517621 MsaT037458.1:CDS
CCTTTCAATTACATCCTTGA+GGG 0.460597 7:+58519194 None:intergenic
ACGAAAGCTGCCAAAGGAAA+AGG 0.462660 7:+58517948 None:intergenic
TTCACATCCACTCTCAGACT+TGG 0.462701 7:-58517696 MsaT037458.1:CDS
TTGGTTGGATTGGAGAAAGT+GGG 0.463944 7:-58523549 MsaT037458.1:CDS
TTTAACTGAAAGCAATAGAA+TGG 0.478373 7:+58517512 None:intergenic
GATGTTCCAAGCCTTGCTAT+AGG 0.480739 7:-58518236 MsaT037458.1:intron
TGTTTAGTGTGGCATTTGCA+TGG 0.482006 7:-58517194 MsaT037458.1:CDS
TTGTTATTGGCTGATTACTC+AGG 0.482236 7:-58519150 MsaT037458.1:intron
AGAAAGTGGGAAAAGGTGAT+TGG 0.482958 7:-58523536 MsaT037458.1:CDS
AAAATGGGCATGCAGGGCCA+GGG 0.483683 7:+58517834 None:intergenic
TGAGTTGCCATCATGCTACT+TGG 0.487325 7:-58517074 MsaT037458.1:CDS
GTGGATTCCTGAGGTTGATC+CGG 0.487895 7:-58517001 MsaT037458.1:CDS
ACAAGTGGTAAATATGCAGC+AGG 0.493189 7:+58518299 None:intergenic
GAGAGATCCCACCAGCAACT+TGG 0.494449 7:+58517613 None:intergenic
TAATTACCTATAGCAAGGCT+TGG 0.495124 7:+58518230 None:intergenic
CACCAGCAACTTGGGAAGCA+AGG 0.497350 7:+58517622 None:intergenic
TTGCTTCCCAAGTTGCTGGT+GGG 0.499786 7:-58517620 MsaT037458.1:CDS
TCAGTTCCATTCATGAAAGC+TGG 0.500506 7:+58518332 None:intergenic
TAAAATCGGCTAAAGCTTTG+AGG 0.500789 7:+58518420 None:intergenic
AAAGGAAAAGGAAGTGTAGC+TGG 0.501361 7:+58517960 None:intergenic
GTGCTCCCAGTCAATCAATC+AGG 0.501419 7:-58519232 MsaT037458.1:CDS
TGGAAGCCAGCTTTCATGAA+TGG 0.503451 7:-58518338 MsaT037458.1:CDS
AACAATTCCGGATCAACCTC+AGG 0.506041 7:+58516994 None:intergenic
AGAGATCCCACCAGCAACTT+GGG 0.507342 7:+58517614 None:intergenic
ACAATTGAATGAAGATGAGT+TGG 0.508831 7:-58516947 MsaT037458.1:CDS
TATGCTTAGCGGCTATGCAC+TGG 0.509897 7:-58517229 MsaT037458.1:CDS
GCCTCTCCATTGTGTTTGCA+AGG 0.514317 7:+58517106 None:intergenic
ACGAACTTCACCACCAGTAA+TGG 0.515475 7:-58519386 MsaT037458.1:CDS
TACCTCTTTATGGATCCAAA+AGG 0.517282 7:-58517811 MsaT037458.1:CDS
AGTGGTGATATCAAAGAGAC+TGG 0.518743 7:+58523404 None:intergenic
TTTATCTGTTGTTCAACAAG+TGG 0.519488 7:+58518284 None:intergenic
CCCTCAAGGATGTAATTGAA+AGG 0.522915 7:-58519194 MsaT037458.1:CDS
ACTCTTAAGTAGAGAATCCC+TGG 0.523402 7:+58517582 None:intergenic
CACTGAACATGATTTGTGTA+TGG 0.525344 7:-58519351 MsaT037458.1:CDS
AAAGTAGTAGTACCTTTGTA+TGG 0.525730 7:+58525361 None:intergenic
GAAGCAACGGCATTGAGAGT+TGG 0.530299 7:+58517864 None:intergenic
TTTGGTTGGATTGGAGAAAG+TGG 0.531455 7:-58523550 MsaT037458.1:CDS
CTGAAGTTACAAGGGGCGTA+TGG 0.533589 7:-58518358 MsaT037458.1:CDS
GTCAAGCAAGCCGCGACACT+CGG 0.535595 7:-58517381 MsaT037458.1:CDS
GAGTTGGGACAATCAGATTG+AGG 0.537050 7:+58517880 None:intergenic
GTTGTGGTGGAAGACAGTTC+CGG 0.537675 7:+58517752 None:intergenic
GCAATGGAAACCAAAGATGC+TGG 0.538275 7:+58518395 None:intergenic
ACAAACTTGACAAAGGCAAT+GGG 0.539105 7:-58517908 MsaT037458.1:CDS
AGCAAGGCCATGACCAAGAA+GGG 0.541204 7:+58517638 None:intergenic
ATCAAATGAAGTTGAATGGT+GGG 0.542099 7:-58524507 MsaT037458.1:CDS
CTCTTGTCAGATACAAGGCA+CGG 0.543493 7:-58517404 MsaT037458.1:CDS
ACCTCAGGAATCCACATTTG+AGG 0.543852 7:+58517009 None:intergenic
TACAAACTTGACAAAGGCAA+TGG 0.544769 7:-58517909 MsaT037458.1:CDS
CTATCCCTCCAATTTCCAAA+AGG 0.545538 7:+58516917 None:intergenic
ATGAATAATAATGGTGGTGT+TGG 0.546260 7:-58517663 MsaT037458.1:CDS
CCAGGGGCAAACAGAAGCAA+CGG 0.547663 7:+58517851 None:intergenic
GCTTTGGTTATGGGTAGCAG+AGG 0.552540 7:-58525392 MsaT037458.1:CDS
GATGATTGTTGATTCAGTTG+GGG 0.554707 7:-58525429 MsaT037458.1:CDS
GTTTAGTGTGGCATTTGCAT+GGG 0.555058 7:-58517193 MsaT037458.1:CDS
AATTGGAGGGATAGGTGTCA+TGG 0.557017 7:-58516908 MsaT037458.1:CDS
TTCTCTACTTAAGAGTGTAC+CGG 0.558997 7:-58517575 MsaT037458.1:CDS
GTATGGAATGAAATGTGGAC+AGG 0.559164 7:-58517430 MsaT037458.1:CDS
ACCTTTCAATTACATCCTTG+AGG 0.562218 7:+58519193 None:intergenic
ACTCGCAATGGCGAGCTCAA+CGG 0.562278 7:+58520949 None:intergenic
GGATTGGAGAAAGTGGGAAA+AGG 0.562648 7:-58523543 MsaT037458.1:CDS
TGGAATCAAATGAAGTTGAA+TGG 0.562909 7:-58524534 MsaT037458.1:CDS
TAGTGGGTTTACCTCAAATG+TGG 0.564770 7:-58517020 MsaT037458.1:CDS
AAGCAAGGCCATGACCAAGA+AGG 0.564852 7:+58517637 None:intergenic
ACATGATTTGTGTATGGTAC+AGG 0.565964 7:-58519345 MsaT037458.1:CDS
ACCTCAAATGTGGATTCCTG+AGG 0.566821 7:-58517010 MsaT037458.1:CDS
GGATCTCTCCAGTTGCTCCA+GGG 0.569027 7:-58517599 MsaT037458.1:CDS
GGTTTCTCTTGTCAGATACA+AGG 0.571357 7:-58517409 MsaT037458.1:CDS
TCCACCAAATCGACGAGCAA+AGG 0.571454 7:-58517151 MsaT037458.1:CDS
GATACGTTAGAACAGGATGA+TGG 0.573139 7:-58517273 MsaT037458.1:CDS
AAGATTAATTACCTATAGCA+AGG 0.575466 7:+58518225 None:intergenic
TTGTGCTCTTCCTCAGTCCA+CGG 0.578432 7:+58523579 None:intergenic
AGCCGAAAAGTAGCTTGCAA+TGG 0.580939 7:+58518379 None:intergenic
AAATGGGCATGCAGGGCCAG+GGG 0.581112 7:+58517835 None:intergenic
GTAGGAACGCTTCGCGATTG+CGG 0.581210 7:+58521037 None:intergenic
ATCAAGTATGGAATGAAATG+TGG 0.582356 7:-58517435 MsaT037458.1:CDS
ACATAATATCTCCAATAGAC+CGG 0.584267 7:+58517556 None:intergenic
GGTTATTAAAGTTCCCCTCA+AGG 0.585039 7:-58519208 MsaT037458.1:CDS
GCTCACAAAACCTGACACAT+AGG 0.585072 7:+58517047 None:intergenic
TCATTGGTTTGGATATCAGG+TGG 0.587552 7:-58517354 MsaT037458.1:CDS
ATCAAATGAAGTTGAATGGT+GGG 0.587690 7:-58524530 MsaT037458.1:CDS
GTGTTGGTTGCTATGCTTAG+CGG 0.587896 7:-58517240 MsaT037458.1:CDS
TGATGGAGAATCTGGAGTGT+TGG 0.592024 7:-58517256 MsaT037458.1:CDS
GGATCCAAAAGGTGAACCGT+TGG 0.592102 7:-58517800 MsaT037458.1:CDS
TATCAAAGAGACTGGATCTA+CGG 0.592657 7:+58523412 None:intergenic
CTACAGCATCTCTGTTGTGG+TGG 0.596787 7:+58517739 None:intergenic
GAACTACAGCATCTCTGTTG+TGG 0.597427 7:+58517736 None:intergenic
CTTTCAATTACATCCTTGAG+GGG 0.598422 7:+58519195 None:intergenic
AATCAAATGAAGTTGAATGG+TGG 0.608049 7:-58524531 MsaT037458.1:CDS
GGAATTCCTTGCAAACACAA+TGG 0.608818 7:-58517112 MsaT037458.1:CDS
TCTTGTCAGATACAAGGCAC+GGG 0.610069 7:-58517403 MsaT037458.1:CDS
CTTTGATATCACCACTGACA+CGG 0.611879 7:-58523397 MsaT037458.1:intron
TGGGATCAAATGAAGTTGAA+TGG 0.615162 7:-58524511 MsaT037458.1:CDS
TTACTGGTGGTGAAGTTCGT+AGG 0.616509 7:+58519389 None:intergenic
GACGGCAATTGAGATTACTT+CGG 0.617287 7:+58523436 None:intergenic
TGTAGAGCCAAGTCTGAGAG+TGG 0.618398 7:+58517689 None:intergenic
AGGCTTGCCAAGTAGCATGA+TGG 0.618629 7:+58517067 None:intergenic
GATCAAATGAAGTTGAATGG+TGG 0.618929 7:-58524508 MsaT037458.1:CDS
GTGATTGGAGAGGAATCTCA+AGG 0.619264 7:-58523521 MsaT037458.1:CDS
ATTGGAGGGATAGGTGTCAT+GGG 0.620668 7:-58516907 MsaT037458.1:CDS
TCAAATGAAGTTGAATGGTG+GGG 0.622134 7:-58524506 MsaT037458.1:CDS
TCCCAAAAGCATAATATCAG+TGG 0.623280 7:+58519314 None:intergenic
GGCAATTGAGATTACTTCGG+CGG 0.623482 7:+58523439 None:intergenic
GTGGGAAAAGGTGATTGGAG+AGG 0.624127 7:-58523531 MsaT037458.1:CDS
GAGGTTTGGGTGCCATACAA+AGG 0.624194 7:-58525373 MsaT037458.1:intron
AACCTCCTGATTGATTGACT+GGG 0.625426 7:+58519227 None:intergenic
TGAATGGATCAGGGATTCCG+TGG 0.626840 7:-58523596 MsaT037458.1:intron
GCGAGCTAAACAACTCGCAA+TGG 0.628105 7:+58520937 None:intergenic
GGTTACTCGCAGGTGCGAGT+AGG 0.629624 7:+58521019 None:intergenic
ATTGTTGAAGAGACTAAGCA+AGG 0.631399 7:-58516977 MsaT037458.1:CDS
GCATGACTTGCAACATGCGT+AGG 0.636946 7:+58523477 None:intergenic
TCCTTGCAAACACAATGGAG+AGG 0.639673 7:-58517107 MsaT037458.1:CDS
CAATGAAGCACCGAGTGTCG+CGG 0.640419 7:+58517371 None:intergenic
AGGCTTACGAAAGCTGCCAA+AGG 0.640426 7:+58517942 None:intergenic
CTCCCAGTCAATCAATCAGG+AGG 0.644545 7:-58519229 MsaT037458.1:CDS
ATAACATACAAACTTGACAA+AGG 0.650531 7:-58517915 MsaT037458.1:CDS
TTCACCACCAGTAATGGAGA+CGG 0.651845 7:-58519380 MsaT037458.1:CDS
TTGTTGAAGAGACTAAGCAA+GGG 0.652825 7:-58516976 MsaT037458.1:CDS
ATCAGGGATTCCGTGGACTG+AGG 0.660333 7:-58523589 MsaT037458.1:intron
ATCGCCGTCTCCATTACTGG+TGG 0.665704 7:+58519376 None:intergenic
GCTTGGAACATCAACACCTG+TGG 0.678556 7:+58518247 None:intergenic
CTGTTATCAAAAGTGTGCGA+AGG 0.678808 7:+58522434 None:intergenic
AGAGACTGGATCTACGGCGA+CGG 0.684123 7:+58523418 None:intergenic
CAAGTGGTAAATATGCAGCA+GGG 0.706180 7:+58518300 None:intergenic
ACATGTATGAATAATAATGG+TGG 0.709661 7:-58517669 MsaT037458.1:CDS
TGTACTGCACTAACTCGCAG+TGG 0.711108 7:+58520915 None:intergenic
TCGCAATGGCGAGCTCAACG+GGG 0.753157 7:+58520951 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 58516864 58525450 58516864 ID=MsaG037458
Chr7 mRNA 58516864 58525450 58516864 ID=MsaT037458.1;Parent=MsaG037458
Chr7 exon 58516864 58517993 58516864 ID=MsaT037458.1.exon11;Parent=MsaT037458.1
Chr7 CDS 58516864 58517993 58516864 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58518237 58518443 58518237 ID=MsaT037458.1.exon10;Parent=MsaT037458.1
Chr7 CDS 58518237 58518443 58518237 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58519151 58519283 58519151 ID=MsaT037458.1.exon9;Parent=MsaT037458.1
Chr7 CDS 58519151 58519283 58519151 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58519313 58519431 58519313 ID=MsaT037458.1.exon8;Parent=MsaT037458.1
Chr7 CDS 58519313 58519431 58519313 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58520894 58520976 58520894 ID=MsaT037458.1.exon7;Parent=MsaT037458.1
Chr7 CDS 58520894 58520976 58520894 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58521025 58521094 58521025 ID=MsaT037458.1.exon6;Parent=MsaT037458.1
Chr7 CDS 58521025 58521094 58521025 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58522435 58522498 58522435 ID=MsaT037458.1.exon5;Parent=MsaT037458.1
Chr7 CDS 58522435 58522498 58522435 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58523398 58523606 58523398 ID=MsaT037458.1.exon4;Parent=MsaT037458.1
Chr7 CDS 58523398 58523606 58523398 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58524488 58524563 58524488 ID=MsaT037458.1.exon3;Parent=MsaT037458.1
Chr7 CDS 58524488 58524563 58524488 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58525266 58525275 58525266 ID=MsaT037458.1.exon2;Parent=MsaT037458.1
Chr7 CDS 58525266 58525275 58525266 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Chr7 exon 58525374 58525450 58525374 ID=MsaT037458.1.exon1;Parent=MsaT037458.1
Chr7 CDS 58525374 58525450 58525374 ID=cds.MsaT037458.1;Parent=MsaT037458.1
Gene Sequence

>MsaT037458.1

ATGATTGTTGATTCAGTTGGGGATTTGAAGCTTGATGCTTTGGTTATGGGTAGCAGAGGTTTGGGTGCCATACAAAGAACTATCCCTCATAAAGTGGAATCAAATGAAGTTGAATGGTGGGATCAAATGAAGTTGAATGGTGGGGCTGAGCAGCAAAAAAGAGGGATTCCGTGGACTGAGGAAGAGCACAAGATGTTTTTGGTTGGATTGGAGAAAGTGGGAAAAGGTGATTGGAGAGGAATCTCAAGGAACTATGTGAAAACAAGAACGCCTACGCATGTTGCAAGTCATGCTCAGAAGTACTTTCTCCGCCGAAGTAATCTCAATTGCCGTCGCCGTAGATCCAGTCTCTTTGATATCACCACTGACACGTACATATTGTTGGCTTTCTTATTTGAATTTTATTCATGGCATCCTTCGCACACTTTTGATAACAATGTGTTGCGAGCTCGGCAGAACCTGTTGCCATCGCCGCAATCGCGAAGCGTTCCTACTCGCACCTGCGATTGCCCCGTTGAGCTCGCCATTGCGAGTTGTTTAGCTCGCCACTGCGAGTTAGTGCAGTACAGACCTGATGTTGTGCGAAATTGTTTAATTTCAGTTCAGTATCCTACGAACTTCACCACCAGTAATGGAGACGGCGATATCACTGAACATGATTTGTGTATGGTACAGGTATCGATACCACTGATATTATGCTTTTGGGAAAAGGAAGCTTCTCGCTTTTATATAATGCAGTGCTCCCAGTCAATCAATCAGGAGGTTATTAAAGTTCCCCTCAAGGATGTAATTGAAAGGTTTGTTTTAATTGTTATCGTTTTGTTATTGGCTGATTACTCAGGCCTCAAAGCTTTAGCCGATTTTATTCCAGCATCTTTGGTTTCCATTGCAAGCTACTTTTCGGCTGAAGTTACAAGGGGCGTATGGAAGCCAGCTTTCATGAATGGAACTGATTGGCCTAGCCCTGCTGCATATTTACCACTTGTTGAACAACAGATAAAGAAAATTTTAGCAGCCACAGGTGTTGATGTTCCAAGCCTTGCTATAGATGAGGACTCTCCAGCTACACTTCCTTTTCCTTTGGCAGCTTTCGTAAGCCTATCAATAACATACAAACTTGACAAAGGCAATGGGAGATTCCTCAATCTGATTGTCCCAACTCTCAATGCCGTTGCTTCTGTTTGCCCCTGGCCCTGCATGCCCATTTTTACCTCTTTATGGATCCAAAAGGTGAACCGTTGGCGTGACTACTTTTTGCTGCAAGCTTCCGGAACTGTCTTCCACCACAACAGAGATGCTGTAGTTCAGCTGCTAAAGAGTTGCTTCACATCCACTCTCAGACTTGGCTCTACATGTATGAATAATAATGGTGGTGTTGGTGCCCTTCTTGGTCATGGCCTTGCTTCCCAAGTTGCTGGTGGGATCTCTCCAGTTGCTCCAGGGATTCTCTACTTAAGAGTGTACCGGTCTATTGGAGATATTATGTTTTTGAACAAAGAAATCTTGTCCATTCTATTGCTTTCAGTTAAAGACATAGCAAGTAGCGAGTTACCTAAAGACAATGTAAAGAAACAACTAAATAAAATCAAGTATGGAATGAAATGTGGACAGGTTTCTCTTGTCAGATACAAGGCACGGGTCAAGCAAGCCGCGACACTCGGTGCTTCATTGGTTTGGATATCAGGTGGAAAAAAGTTGGTTCAATCATTGATAGGAGACACTGTTCCTTCCTGGTTTTTATCAGCTGATACGTTAGAACAGGATGATGGAGAATCTGGAGTGTTGGTTGCTATGCTTAGCGGCTATGCACTGGCGTATTTTGTTATGTTTAGTGTGGCATTTGCATGGGATATTGACAACTATTCAGTTCCACCAAATCGACGAGCAAAGGTTATTGCAATTCATTTGGAATTCCTTGCAAACACAATGGAGAGGCTTACATCACTGAGTTGCCATCATGCTACTTGGCAAGCCTATGTGTCAGGTTTTGTGAGCTTGATAGTGGGTTTACCTCAAATGTGGATTCCTGAGGTTGATCCGGAATTGTTGAAGAGACTAAGCAAGGGATTAAGACAATTGAATGAAGATGAGTTGGCTCTTCGCCTTTTGGAAATTGGAGGGATAGGTGTCATGGGTGCAGCAGCTGAAATGATAATAGCATCTGAAAGTATGTTGTAA
Protein sequence

>MsaT037458.1

MIVDSVGDLKLDALVMGSRGLGAIQRTIPHKVESNEVEWWDQMKLNGGAEQQKRGIPWTEEEHKMFLVGLEKVGKGDWRGISRNYVKTRTPTHVASHAQKYFLRRSNLNCRRRRSSLFDITTDTYILLAFLFEFYSWHPSHTFDNNVLRARQNLLPSPQSRSVPTRTCDCPVELAIASCLARHCELVQYRPDVVRNCLISVQYPTNFTTSNGDGDITEHDLCMVQVSIPLILCFWEKEASRFYIMQCSQSINQEVIKVPLKDVIERFVLIVIVLLLADYSGLKALADFIPASLVSIASYFSAEVTRGVWKPAFMNGTDWPSPAAYLPLVEQQIKKILAATGVDVPSLAIDEDSPATLPFPLAAFVSLSITYKLDKGNGRFLNLIVPTLNAVASVCPWPCMPIFTSLWIQKVNRWRDYFLLQASGTVFHHNRDAVVQLLKSCFTSTLRLGSTCMNNNGGVGALLGHGLASQVAGGISPVAPGILYLRVYRSIGDIMFLNKEILSILLLSVKDIASSELPKDNVKKQLNKIKYGMKCGQVSLVRYKARVKQAATLGASLVWISGGKKLVQSLIGDTVPSWFLSADTLEQDDGESGVLVAMLSGYALAYFVMFSVAFAWDIDNYSVPPNRRAKVIAIHLEFLANTMERLTSLSCHHATWQAYVSGFVSLIVGLPQMWIPEVDPELLKRLSKGLRQLNEDELALRLLEIGGIGVMGAAAEMIIASESML*