Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037477 | KEH22681.1 | 96.075 | 586 | 23 | 0 | 1 | 586 | 164 | 749 | 0.0 | 1184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037477 | sp|Q9SXB8|Y1133_ARATH | 52.211 | 588 | 267 | 7 | 1 | 582 | 181 | 760 | 0.0 | 629 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037477 | tr|A0A072TZR6|A0A072TZR6_MEDTR | 96.075 | 586 | 23 | 0 | 1 | 586 | 164 | 749 | 0.0 | 1184 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037477 | MtrunA17_Chr7g0236231 | 96.075 | 586 | 23 | 0 | 1 | 586 | 174 | 759 | 0.0 | 1183 |
| MsaG037477 | MtrunA17_Chr7g0236121 | 64.856 | 589 | 182 | 7 | 4 | 586 | 175 | 744 | 0.0 | 788 |
| MsaG037477 | MtrunA17_Chr7g0236201 | 63.311 | 586 | 162 | 5 | 4 | 586 | 175 | 710 | 0.0 | 759 |
| MsaG037477 | MtrunA17_Chr7g0236341 | 61.667 | 600 | 208 | 5 | 3 | 589 | 171 | 761 | 0.0 | 751 |
| MsaG037477 | MtrunA17_Chr7g0236551 | 60.988 | 587 | 202 | 3 | 1 | 586 | 169 | 729 | 0.0 | 741 |
| MsaG037477 | MtrunA17_Chr7g0236641 | 61.833 | 600 | 213 | 5 | 1 | 586 | 169 | 766 | 0.0 | 738 |
| MsaG037477 | MtrunA17_Chr7g0236691 | 62.437 | 591 | 203 | 4 | 3 | 590 | 171 | 745 | 0.0 | 737 |
| MsaG037477 | MtrunA17_Chr7g0236471 | 62.203 | 590 | 198 | 7 | 1 | 586 | 13 | 581 | 0.0 | 736 |
| MsaG037477 | MtrunA17_Chr7g0236651 | 60.067 | 596 | 207 | 7 | 1 | 594 | 167 | 733 | 0.0 | 730 |
| MsaG037477 | MtrunA17_Chr7g0236561 | 59.934 | 609 | 225 | 5 | 1 | 594 | 149 | 753 | 0.0 | 729 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037477 | AT1G11330.1 | 52.568 | 584 | 269 | 6 | 1 | 582 | 181 | 758 | 0.0 | 629 |
| MsaG037477 | AT1G11330.2 | 52.211 | 588 | 267 | 7 | 1 | 582 | 181 | 760 | 0.0 | 629 |
| MsaG037477 | AT1G11330.3 | 52.724 | 569 | 255 | 7 | 1 | 563 | 181 | 741 | 0.0 | 610 |
| MsaG037477 | AT1G11300.1 | 48.471 | 654 | 282 | 13 | 1 | 621 | 174 | 805 | 0.0 | 594 |
| MsaG037477 | AT1G11300.2 | 48.239 | 653 | 270 | 14 | 1 | 621 | 174 | 790 | 0.0 | 584 |
| MsaG037477 | AT1G11350.1 | 51.017 | 590 | 272 | 10 | 1 | 583 | 170 | 749 | 0.0 | 574 |
| MsaG037477 | AT1G11303.1 | 50.594 | 589 | 262 | 10 | 1 | 583 | 174 | 739 | 0.0 | 568 |
| MsaG037477 | AT4G21390.1 | 48.154 | 596 | 276 | 12 | 5 | 580 | 180 | 762 | 0.0 | 549 |
| MsaG037477 | AT1G61370.1 | 47.774 | 584 | 281 | 8 | 3 | 580 | 170 | 735 | 0.0 | 549 |
| MsaG037477 | AT1G61370.2 | 47.774 | 584 | 281 | 8 | 3 | 580 | 19 | 584 | 0.0 | 548 |
Find 111 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGACTTTCGAGATCATC+CGG | 0.487705 | 7:-58972188 | MsaT037477.1:CDS |
| AAAATCTTGGAGTATTGAAA+AGG | 0.502539 | 7:-58973358 | MsaT037477.1:CDS |
| AAACGCTTTAGTATAATAGA+AGG | 0.447930 | 7:-58971821 | MsaT037477.1:CDS |
| AAACTTGGACAAGGAGGTTT+TGG | 0.261465 | 7:-58972334 | MsaT037477.1:CDS |
| AAAGAGAAGCTTCCGTTGGA+TGG | 0.488539 | 7:+58973618 | None:intergenic |
| AAATCAGACGTCTTCAGCTT+TGG | 0.376486 | 7:-58971489 | MsaT037477.1:CDS |
| AAATCAGATTGTGATCTTTA+TGG | 0.106758 | 7:-58973306 | MsaT037477.1:CDS |
| AACCAAGAAACAATCAAGAG+TGG | 0.602313 | 7:-58973215 | MsaT037477.1:CDS |
| AACGCACGTTGATTTGATTC+TGG | 0.280014 | 7:-58970216 | MsaT037477.1:CDS |
| AAGAAAATCGGCCATATTGG+CGG | 0.538265 | 7:-58973554 | MsaT037477.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 58969104 | 58973673 | 58969104 | ID=MsaG037477 |
| Chr7 | mRNA | 58969104 | 58973673 | 58969104 | ID=MsaT037477.1;Parent=MsaG037477 |
| Chr7 | exon | 58969104 | 58969254 | 58969104 | ID=MsaT037477.1.exon9;Parent=MsaT037477.1 |
| Chr7 | CDS | 58969104 | 58969254 | 58969104 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58970131 | 58970239 | 58970131 | ID=MsaT037477.1.exon8;Parent=MsaT037477.1 |
| Chr7 | CDS | 58970131 | 58970239 | 58970131 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58971250 | 58971313 | 58971250 | ID=MsaT037477.1.exon7;Parent=MsaT037477.1 |
| Chr7 | CDS | 58971250 | 58971313 | 58971250 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58971407 | 58971557 | 58971407 | ID=MsaT037477.1.exon6;Parent=MsaT037477.1 |
| Chr7 | CDS | 58971407 | 58971557 | 58971407 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58971638 | 58971875 | 58971638 | ID=MsaT037477.1.exon5;Parent=MsaT037477.1 |
| Chr7 | CDS | 58971638 | 58971875 | 58971638 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58972033 | 58972243 | 58972033 | ID=MsaT037477.1.exon4;Parent=MsaT037477.1 |
| Chr7 | CDS | 58972033 | 58972243 | 58972033 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58972321 | 58972526 | 58972321 | ID=MsaT037477.1.exon3;Parent=MsaT037477.1 |
| Chr7 | CDS | 58972321 | 58972526 | 58972321 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58972636 | 58972740 | 58972636 | ID=MsaT037477.1.exon2;Parent=MsaT037477.1 |
| Chr7 | CDS | 58972636 | 58972740 | 58972636 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Chr7 | exon | 58972899 | 58973673 | 58972899 | ID=MsaT037477.1.exon1;Parent=MsaT037477.1 |
| Chr7 | CDS | 58972899 | 58973673 | 58972899 | ID=cds.MsaT037477.1;Parent=MsaT037477.1 |
| Gene Sequence |
>MsaT037477.1 ATGACAATCACATCATGGAAAACACCTACTGATCCATCCAACGGAAGCTTCTCTTTAAGCATTGAACGGTTGAATATGCCAGAAGTTTTTATCAGGAAAGAAAATCGGCCATATTGGCGGAGTGGTCCTTGGAACAATCAAATCTTTATAGGGATAGAAGACATGTCAGCTCTTTATCTTAATGGTTTTCATTTCGGAAAAGATAGAACCGGAGGAACTCTTGATTTAGTCTTCAGAGCAGACGACTATGGACTTGTGATGTATGTTCTTAATTCGACAGGTCAAATGAATGAAAAATCTTGGAGTATTGAAAAGGAGGAATGGATGGATACATGGACTAATCAAAAATCAGATTGTGATCTTTATGGTTTTTGTGGTTCATTTGGAATTTGTAATTCAAAAGGGTCCCCAATTTGTCGCTGTTTGGAAGGGTTTGAACCAAGAAACAATCAAGAGTGGAATAAACAAAATTGGACTAATGGATGTGTTAGGAAGACGCCGTTGCAGTGTGAAAGTGCCAACAATCAAAATAAGAGCGCAAATGGTAATGAAGCAGTTAGTTTTATGAAATTGAGTCTGGTAAAAGTACCTGATTTTGCAGAATTGTCATCTGTTGAACAAGATGAATGCAAAAACCAGTGTTTGATGAATTGCTCTTGCACTGCATATTCATATGTAGCTGATATTGGTTGTATTTCTTGGAATGGAAATCTAATCGATATTCAACAATTCGAAACTGGAGGAACTGATCTATACATTCGTGTACCATCATCAGATAAAGGGAACAATGGAACAGTTATCATTACAGTTTCAGTACTAGTAGGAATTATCATATTTGCTACTGCGGCATATTTCATTTGGATAAAGGTTTCAAAGCCAGTGAGAAATAAGAAGAAACATAGAATTTTTCAATTCAACAAAATTGAAAAAGATGAAGAATACAAAACTGACAATGTAATTGGAGAACTGTCACAAGCAAAGCTACAAGAGCTATTACTATATAGTTTTGAAAAGCTTGCAATTGCCACAAACAACTTCCATTTATCCAATAAACTTGGACAAGGAGGTTTTGGTCCTGTATACAAGGGAATACTGCAAGATGGTAAGGAAATAGCGGTTAAAAGACTTTCGAGATCATCCGGACAAGGGTTAGAAGAATTCATGAATGAAGTAGTAGTGATTTCTAAGCTTCAGCACCGAAATCTTGTAAGACTTGTAGGCTGCTGCATTGAAGGAGACGAAAAGATGTTGATGTATGAGTTCATGCCAAATAGCAGTCTGGATGCATATGTTTTTGATCCATCAAGAAATAAACTTCTGGATTGGGAGAAACGCTTTAGTATAATAGAAGGAATAGCTCGTGGATTGCTTTATCTACACAGGGATTCCAGGCTAAAGATCATACATAGAGATATGAAAGCAAGTAATATCTTGCTAGACGAGAACCTGAATCCAAAAATATCTGACTTTGGTATGGCCAGAATCTTTGGAGTGAGTGAAGATCATGCCAATACTCAAAGGGTTGTCGGAACATATGGTTATATGGCTCCAGAGTATGCAATGCAAGGAGTATTTTCGGATAAATCAGACGTCTTCAGCTTTGGAGTTTTGCTAATAGAGATTGTTAGTGGAAGACGAAACTCAAGCTTTTATGAACATGAAAATTCTCTCACTCTCTTAGGATTTGCTTGGATTCAATGGAAGGAAGACAATATTTTATCTTTGATAGAACCAGAAATATATGATCATAAAACGCACGTTGATTTGATTCTGGAATTCCTAACTCCTACAAAACCAAGTCAATTTTGCTTTGTAGCGCGAGAATGTATTCCACGACTCATATCACAACATGCTAATAACATATTCTCATTACTAAGTCTGGAAGTTACTCCTCGACGCTTAATTCGGTCATATAGATTCTACTCCCAGCTCTTCAGCTTCAATGAGAGACTTATGACATCATTTATGACACGACTCATCTTATTACGAAGACCAGAAGATCTTCCCTAG |
| Protein sequence |
>MsaT037477.1 MTITSWKTPTDPSNGSFSLSIERLNMPEVFIRKENRPYWRSGPWNNQIFIGIEDMSALYLNGFHFGKDRTGGTLDLVFRADDYGLVMYVLNSTGQMNEKSWSIEKEEWMDTWTNQKSDCDLYGFCGSFGICNSKGSPICRCLEGFEPRNNQEWNKQNWTNGCVRKTPLQCESANNQNKSANGNEAVSFMKLSLVKVPDFAELSSVEQDECKNQCLMNCSCTAYSYVADIGCISWNGNLIDIQQFETGGTDLYIRVPSSDKGNNGTVIITVSVLVGIIIFATAAYFIWIKVSKPVRNKKKHRIFQFNKIEKDEEYKTDNVIGELSQAKLQELLLYSFEKLAIATNNFHLSNKLGQGGFGPVYKGILQDGKEIAVKRLSRSSGQGLEEFMNEVVVISKLQHRNLVRLVGCCIEGDEKMLMYEFMPNSSLDAYVFDPSRNKLLDWEKRFSIIEGIARGLLYLHRDSRLKIIHRDMKASNILLDENLNPKISDFGMARIFGVSEDHANTQRVVGTYGYMAPEYAMQGVFSDKSDVFSFGVLLIEIVSGRRNSSFYEHENSLTLLGFAWIQWKEDNILSLIEPEIYDHKTHVDLILEFLTPTKPSQFCFVARECIPRLISQHANNIFSLLSLEVTPRRLIRSYRFYSQLFSFNERLMTSFMTRLILLRRPEDLP* |
