Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037490 | XP_024637264.1 | 91.463 | 82 | 7 | 0 | 27 | 108 | 84 | 165 | 2.34e-49 | 164 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037490 | tr|G7JQ00|G7JQ00_MEDTR | 91.463 | 82 | 7 | 0 | 27 | 108 | 144 | 225 | 6.37e-49 | 164 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG037490 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAACTTTAGTGGTAAAATT+TGG | 0.131735 | 7:+59214094 | None:intergenic |
| TAACTTTAGTGGTAAAATTT+GGG | 0.245976 | 7:+59214095 | None:intergenic |
| GAACGAACATTTAACTTTAG+TGG | 0.296028 | 7:+59214084 | None:intergenic |
| CACAGTGCATGTAGAAAATC+AGG | 0.323162 | 7:-59214059 | MsaT037490.1:intron |
| AGGAATTTGCCGAAAGTATC+AGG | 0.335653 | 7:+59213407 | None:intergenic |
| AATCGTGATGTTTCACTCTA+AGG | 0.341127 | 7:+59213387 | None:intergenic |
| ATAGGAAGATATGCGTTTAA+AGG | 0.361554 | 7:-59214128 | None:intergenic |
| TAGGAACTACCTGATACTTT+CGG | 0.400617 | 7:-59213416 | MsaT037490.1:CDS |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 7:+59213301 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 7:+59213302 | None:intergenic |
| TTGTGCTGCCAATGGTATTG+AGG | 0.463000 | 7:-59213261 | MsaT037490.1:CDS |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 7:-59213296 | MsaT037490.1:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 7:-59213302 | MsaT037490.1:CDS |
| GTCATGCGTTTATCAACGCT+TGG | 0.494559 | 7:+59213178 | None:intergenic |
| TGTGCTGCCAATGGTATTGA+GGG | 0.501205 | 7:-59213260 | MsaT037490.1:CDS |
| AACTTTAGTGGTAAAATTTG+GGG | 0.508761 | 7:+59214096 | None:intergenic |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 7:+59213303 | None:intergenic |
| TTTCAAGTGCAAGTGCGTCA+TGG | 0.567746 | 7:-59213222 | MsaT037490.1:CDS |
| GTCTCCGCCCTCAATACCAT+TGG | 0.581950 | 7:+59213253 | None:intergenic |
| CACGATTACGTCATGTTGTG+CGG | 0.585634 | 7:-59213371 | MsaT037490.1:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 7:-59213292 | MsaT037490.1:CDS |
| ACTTTAGTGGTAAAATTTGG+GGG | 0.613670 | 7:+59214097 | None:intergenic |
| GCTGCCAATGGTATTGAGGG+CGG | 0.739556 | 7:-59213257 | MsaT037490.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 59213190 | 59214140 | 59213190 | ID=MsaG037490 |
| Chr7 | mRNA | 59213190 | 59214140 | 59213190 | ID=MsaT037490.1;Parent=MsaG037490 |
| Chr7 | exon | 59213190 | 59213435 | 59213190 | ID=MsaT037490.1.exon2;Parent=MsaT037490.1 |
| Chr7 | CDS | 59213190 | 59213435 | 59213190 | ID=cds.MsaT037490.1;Parent=MsaT037490.1 |
| Chr7 | exon | 59214060 | 59214140 | 59214060 | ID=MsaT037490.1.exon1;Parent=MsaT037490.1 |
| Chr7 | CDS | 59214060 | 59214140 | 59214060 | ID=cds.MsaT037490.1;Parent=MsaT037490.1 |
| Gene Sequence |
| Protein sequence |
