Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037525 | PNX76213.1 | 42.188 | 128 | 74 | 0 | 32 | 159 | 95 | 222 | 8.28e-26 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037525 | tr|A0A2K3LCF2|A0A2K3LCF2_TRIPR | 42.188 | 128 | 74 | 0 | 32 | 159 | 95 | 222 | 3.96e-26 | 108 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG037525 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTATACGATTTACTATTA+TGG | 0.150896 | 7:+59870297 | MsaT037525.1:CDS |
| TTGTTCTGAGAACCACTTTA+AGG | 0.164196 | 7:+59870858 | MsaT037525.1:CDS |
| AGAAGATCATGGACTGAATT+TGG | 0.168545 | 7:+59870817 | MsaT037525.1:CDS |
| TAGCGATATGTGTGATAAAA+TGG | 0.269152 | 7:-59870508 | None:intergenic |
| GGACTGAATTTGGGGATAAA+TGG | 0.270320 | 7:+59870827 | MsaT037525.1:CDS |
| ACATCAACTTTCAATCCTTC+TGG | 0.291898 | 7:-59870769 | None:intergenic |
| GGTATACGATTTACTATTAT+GGG | 0.296382 | 7:+59870298 | MsaT037525.1:CDS |
| GAAGATCATGGACTGAATTT+GGG | 0.307959 | 7:+59870818 | MsaT037525.1:CDS |
| TAATGTTCTCGCACAAAGTT+TGG | 0.345237 | 7:-59870265 | None:intergenic |
| TTGGTTAGTTTAACTTCAAA+AGG | 0.387339 | 7:-59870550 | None:intergenic |
| AGTTTAACTTCAAAAGGCTC+AGG | 0.471079 | 7:-59870544 | None:intergenic |
| ATTACCACGGAGTAATATCT+AGG | 0.481301 | 7:-59870739 | None:intergenic |
| ACACATCTCCTTCCTTAAAG+TGG | 0.483854 | 7:-59870870 | None:intergenic |
| TGATAAAATGGTAATATCAA+TGG | 0.485102 | 7:-59870496 | None:intergenic |
| TCTAGGAGTTCTAAGATAGT+TGG | 0.488890 | 7:-59870722 | None:intergenic |
| ATACTTCTCAGCATAATACT+TGG | 0.490669 | 7:-59870569 | None:intergenic |
| TATCACACATATCGCTATGT+TGG | 0.509609 | 7:+59870514 | MsaT037525.1:CDS |
| CATCATAACTTTCATCACAT+TGG | 0.526559 | 7:-59870923 | None:intergenic |
| GATAAAATGGTAATATCAAT+GGG | 0.532657 | 7:-59870495 | None:intergenic |
| GGAACAAAGGAAGAAGATCA+TGG | 0.533237 | 7:+59870806 | MsaT037525.1:CDS |
| CGAGAACATTATCAACAACT+TGG | 0.533791 | 7:+59870277 | MsaT037525.1:CDS |
| CACATATCGCTATGTTGGAC+TGG | 0.537867 | 7:+59870519 | MsaT037525.1:CDS |
| TTTGGTTCAACTTCACCGTA+TGG | 0.547931 | 7:-59870247 | None:intergenic |
| TTGATGTCAAGATTCTAAAG+AGG | 0.554344 | 7:+59870785 | MsaT037525.1:CDS |
| CAATGTGATGAAAGTTATGA+TGG | 0.563035 | 7:+59870924 | MsaT037525.1:CDS |
| TCTGAGAACCACTTTAAGGA+AGG | 0.566120 | 7:+59870862 | MsaT037525.1:CDS |
| GTGGTAATGTGTGGACCAGA+AGG | 0.600116 | 7:+59870754 | MsaT037525.1:CDS |
| AAGATTCTAAAGAGGAACAA+AGG | 0.604461 | 7:+59870793 | MsaT037525.1:CDS |
| TATTACTCCGTGGTAATGTG+TGG | 0.612024 | 7:+59870745 | MsaT037525.1:CDS |
| AAGATCATGGACTGAATTTG+GGG | 0.613647 | 7:+59870819 | MsaT037525.1:CDS |
| ATCAATGGGCTGAATTCTTG+TGG | 0.614660 | 7:-59870481 | None:intergenic |
| ACAAACGAGTTGCAGCCATA+CGG | 0.621234 | 7:+59870232 | MsaT037525.1:CDS |
| TTCTGGTCCACACATTACCA+CGG | 0.702936 | 7:-59870752 | None:intergenic |
| AACTCCTAGATATTACTCCG+TGG | 0.711344 | 7:+59870735 | MsaT037525.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 59870214 | 59870951 | 59870214 | ID=MsaG037525 |
| Chr7 | mRNA | 59870214 | 59870951 | 59870214 | ID=MsaT037525.1;Parent=MsaG037525 |
| Chr7 | exon | 59870214 | 59870319 | 59870214 | ID=MsaT037525.1.exon1;Parent=MsaT037525.1 |
| Chr7 | CDS | 59870214 | 59870319 | 59870214 | ID=cds.MsaT037525.1;Parent=MsaT037525.1 |
| Chr7 | exon | 59870467 | 59870597 | 59870467 | ID=MsaT037525.1.exon2;Parent=MsaT037525.1 |
| Chr7 | CDS | 59870467 | 59870597 | 59870467 | ID=cds.MsaT037525.1;Parent=MsaT037525.1 |
| Chr7 | exon | 59870703 | 59870951 | 59870703 | ID=MsaT037525.1.exon3;Parent=MsaT037525.1 |
| Chr7 | CDS | 59870703 | 59870951 | 59870703 | ID=cds.MsaT037525.1;Parent=MsaT037525.1 |
| Gene Sequence |
| Protein sequence |
