AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037566

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037566 MtrunA17_Chr7g0237271 98.980 784 8 0 1 784 1 784 0.0 1615
MsaG037566 MtrunA17_Chr7g0237241 94.388 784 35 1 1 784 1 775 0.0 1431
MsaG037566 MtrunA17_Chr1g0171711 63.579 788 273 12 8 784 2 786 0.0 957
MsaG037566 MtrunA17_Chr1g0148791 43.010 937 338 25 8 784 2 902 0.0 610
MsaG037566 MtrunA17_Chr3g0138311 41.335 779 313 24 114 784 155 897 2.99e-157 480
MsaG037566 MtrunA17_Chr2g0282401 42.655 354 193 6 437 784 439 788 1.87e-92 306
MsaG037566 MtrunA17_Chr4g0062881 35.887 496 268 12 298 784 3 457 8.79e-88 285
MsaG037566 MtrunA17_Chr4g0047051 36.823 277 172 3 510 784 33 308 2.83e-58 201
MsaG037566 MtrunA17_Chr4g0047071 37.324 284 164 6 512 784 2 282 7.25e-52 182
MsaG037566 MtrunA17_Chr4g0047091 36.170 282 175 4 506 784 87 366 1.78e-50 181
MsaG037566 MtrunA17_Chr4g0047061 37.698 252 150 5 536 784 83 330 5.10e-46 167
MsaG037566 MtrunA17_Chr4g0047101 37.768 233 127 5 509 736 28 247 1.32e-40 150
MsaG037566 MtrunA17_Chr4g0047111 36.986 146 68 3 591 735 7 129 2.14e-24 100
MsaG037566 MtrunA17_Chr4g0062891 37.589 141 82 4 18 154 22 160 2.24e-24 102
MsaG037566 MtrunA17_Chr1g0172501 24.055 291 211 7 500 783 265 552 1.01e-20 97.1
MsaG037566 MtrunA17_Chr3g0093681 24.055 291 211 7 500 783 265 552 1.34e-20 96.7
MsaG037566 MtrunA17_Chr5g0405851 23.948 309 204 9 500 783 147 449 1.10e-19 92.8
MsaG037566 MtrunA17_Chr4g0067151 23.418 316 185 9 483 783 4 277 2.51e-18 87.0
MsaG037566 MtrunA17_Chr4g0067151 23.457 243 174 6 510 742 81 321 3.42e-12 68.6
MsaG037566 MtrunA17_Chr1g0185501 25.436 287 166 8 504 783 23 268 5.74e-18 85.9
MsaG037566 MtrunA17_Chr4g0053651 25.882 255 170 8 510 750 292 541 1.18e-16 84.3
MsaG037566 MtrunA17_Chr4g0053651 21.824 307 203 11 509 783 232 533 5.41e-11 66.2
MsaG037566 MtrunA17_Chr8g0352821 19.728 294 225 8 498 783 174 464 6.39e-15 79.0
MsaG037566 MtrunA17_Chr6g0448921 25.000 216 156 4 520 731 30 243 1.49e-14 77.8
MsaG037566 MtrunA17_Chr1g0197551 23.529 272 172 7 510 748 54 322 1.79e-13 74.7
MsaG037566 MtrunA17_Chr1g0168391 26.738 187 131 4 521 702 238 423 5.54e-13 72.4
MsaG037566 MtrunA17_Chr4g0035611 22.426 272 175 8 510 748 54 322 5.62e-13 73.2
MsaG037566 MtrunA17_Chr2g0331981 23.923 209 153 3 510 712 60 268 1.54e-12 71.6
MsaG037566 MtrunA17_Chr2g0281451 23.611 216 159 4 520 731 29 242 1.77e-12 71.2
MsaG037566 MtrunA17_Chr8g0383711 21.631 282 210 7 510 784 21 298 2.11e-12 68.9
MsaG037566 MtrunA17_Chr4g0004241 23.923 209 153 3 510 712 60 268 2.17e-12 70.9
MsaG037566 MtrunA17_Chr3g0136971 27.746 173 119 4 499 667 452 622 8.13e-12 68.9
MsaG037566 MtrunA17_Chr3g0136971 23.144 229 163 6 510 729 422 646 1.27e-11 68.2
MsaG037566 MtrunA17_Chr3g0136971 20.833 288 207 6 501 783 343 614 5.93e-11 66.2
MsaG037566 MtrunA17_Chr5g0401661 23.320 253 175 8 510 748 399 646 1.94e-11 67.8
MsaG037566 MtrunA17_Chr5g0401661 21.290 310 207 10 507 783 335 640 5.67e-11 66.2
MsaG037566 MtrunA17_Chr4g0059031 20.070 284 208 5 518 783 371 653 2.65e-11 67.4
MsaG037566 MtrunA17_Chr1g0200901 22.909 275 198 7 517 783 91 359 4.83e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037566 AT2G32700.6 61.294 788 290 13 8 784 2 785 0.0 926
MsaG037566 AT2G32700.2 61.139 790 290 14 8 784 2 787 0.0 921
MsaG037566 AT2G32700.5 61.139 790 290 14 8 784 2 787 0.0 921
MsaG037566 AT2G32700.1 61.139 790 290 14 8 784 2 787 0.0 921
MsaG037566 AT2G32700.4 61.139 790 290 14 8 784 2 787 0.0 921
MsaG037566 AT2G32700.3 61.139 790 290 14 8 784 2 787 0.0 921
MsaG037566 AT2G32700.7 59.703 809 290 15 8 784 2 806 0.0 909
MsaG037566 AT4G32551.1 54.762 420 173 6 373 784 521 931 6.44e-145 449
MsaG037566 AT4G32551.1 83.908 87 14 0 8 94 2 88 1.17e-42 167
MsaG037566 AT4G32551.1 37.674 215 95 10 113 303 185 384 2.16e-17 87.8
MsaG037566 AT4G32551.2 54.762 420 173 6 373 784 559 969 2.12e-144 449
MsaG037566 AT4G32551.2 83.908 87 14 0 8 94 2 88 1.38e-42 167
MsaG037566 AT4G32551.2 35.124 242 102 12 113 303 185 422 5.70e-15 79.7
MsaG037566 AT2G41500.1 25.979 281 198 7 510 783 258 535 3.06e-21 99.0
MsaG037566 AT2G41500.2 25.979 281 198 7 510 783 258 535 3.06e-21 99.0
MsaG037566 AT4G02730.1 24.055 291 166 6 499 783 44 285 6.62e-19 89.4
MsaG037566 AT3G49660.1 26.224 286 159 8 510 784 27 271 1.75e-18 87.8
MsaG037566 AT3G49660.1 22.041 245 179 7 507 742 71 312 1.20e-11 67.0
MsaG037566 AT5G13480.1 22.789 294 216 9 498 783 114 404 7.18e-18 88.6
MsaG037566 AT5G13480.4 22.789 294 216 9 498 783 102 392 8.78e-18 88.2
MsaG037566 AT5G13480.3 22.789 294 216 9 498 783 102 392 1.06e-17 88.2
MsaG037566 AT5G13480.2 22.789 294 216 9 498 783 125 415 1.28e-17 87.8
MsaG037566 AT1G11160.1 22.685 216 161 4 520 731 29 242 4.92e-14 76.6
MsaG037566 AT1G11160.2 22.685 216 161 4 520 731 29 242 5.69e-14 76.6
MsaG037566 AT1G11160.4 22.685 216 161 4 520 731 29 242 6.64e-14 76.3
MsaG037566 AT1G61210.3 23.077 273 193 9 520 783 29 293 7.09e-14 76.3
MsaG037566 AT1G61210.1 23.077 273 193 9 520 783 29 293 1.23e-13 75.5
MsaG037566 AT1G61210.2 23.077 273 193 9 520 783 29 293 1.30e-13 75.5
MsaG037566 AT5G25150.1 30.058 173 115 5 499 667 451 621 1.49e-13 75.1
MsaG037566 AT5G25150.2 30.058 173 115 5 499 667 451 621 1.67e-13 74.7
MsaG037566 AT5G08390.1 24.352 193 140 4 520 708 30 220 2.27e-13 74.7
MsaG037566 AT2G21390.1 22.441 254 162 7 510 730 54 305 2.41e-13 74.7
MsaG037566 AT1G62020.1 22.426 272 175 7 510 748 54 322 2.43e-13 74.7
MsaG037566 AT5G23430.3 24.352 193 140 4 520 708 30 220 3.30e-13 73.9
MsaG037566 AT5G23430.2 24.352 193 140 4 520 708 30 220 3.30e-13 73.9
MsaG037566 AT5G23430.1 24.352 193 140 4 520 708 30 220 3.31e-13 73.9
MsaG037566 AT2G33340.2 25.781 256 173 8 521 769 236 481 7.02e-13 72.4
MsaG037566 AT2G33340.1 25.781 256 173 8 521 769 236 481 7.02e-13 72.4
MsaG037566 AT2G33340.3 25.781 256 173 8 521 769 236 481 1.08e-12 71.6
MsaG037566 AT1G52360.4 23.923 209 153 3 510 712 60 268 1.09e-12 72.4
MsaG037566 AT1G52360.1 23.923 209 153 3 510 712 60 268 1.09e-12 72.4
MsaG037566 AT1G52360.3 23.923 209 153 3 510 712 60 268 1.30e-12 72.0
MsaG037566 AT3G15980.3 24.402 209 152 3 510 712 60 268 1.38e-12 72.0
MsaG037566 AT3G15980.6 24.402 209 152 3 510 712 60 268 1.38e-12 72.0
MsaG037566 AT3G15980.2 24.402 209 152 3 510 712 60 268 1.38e-12 72.0
MsaG037566 AT3G15980.4 24.402 209 152 3 510 712 60 268 1.38e-12 72.0
MsaG037566 AT3G15980.5 24.402 209 152 3 510 712 60 268 1.53e-12 72.0
MsaG037566 AT3G15980.7 24.402 209 152 3 510 712 60 268 1.55e-12 72.0
MsaG037566 AT3G15980.1 24.402 209 152 3 510 712 60 268 1.65e-12 71.6
MsaG037566 AT1G52360.2 23.923 209 153 3 510 712 104 312 2.52e-12 71.2
MsaG037566 AT5G16750.1 21.631 282 202 5 520 783 372 652 3.76e-12 70.5
MsaG037566 AT1G71840.1 25.000 280 192 11 517 783 81 355 1.26e-11 67.8
MsaG037566 AT5G67320.1 24.204 314 187 9 509 783 267 568 1.36e-11 68.6
MsaG037566 AT1G79990.1 23.445 209 154 3 510 712 60 268 4.00e-11 67.4
MsaG037566 AT1G79990.2 23.445 209 154 3 510 712 60 268 4.73e-11 67.0
MsaG037566 AT5G60940.1 23.478 230 147 10 544 748 118 343 9.62e-11 65.1

Find 181 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATATCTTCACCAAATT+TGG 0.073622 7:+60985408 None:intergenic
ATGGATCCACGCAGACTTTC+TGG 0.168111 7:-60988741 MsaT037566.1:CDS
GGCATCTATTAGTCCAAATT+TGG 0.206338 7:-60985421 MsaT037566.1:CDS
TGGTTCTGCGTTGTTAAATT+AGG 0.217015 7:+60988849 None:intergenic
CAGTTGGTTCATGGTAATTC+TGG 0.222193 7:-60990132 MsaT037566.1:CDS
AGCCTTGGAAATTCTAATTA+TGG 0.228558 7:-60988768 MsaT037566.1:CDS
TGGGTCAAGCTCAAAGTAAT+TGG 0.252250 7:-60991545 MsaT037566.1:CDS
AGAAAGCAAGCTTCTTCTTC+TGG 0.265001 7:-60987928 MsaT037566.1:CDS
GAATCCATGGGAAAATTCTT+TGG 0.267475 7:+60989910 None:intergenic
TTGAGTGGTGGTCCGTCTTT+TGG 0.276468 7:-60991059 MsaT037566.1:CDS
TCAGGTTACCCTGTGGAATA+TGG 0.285489 7:-60986508 MsaT037566.1:intron
ATAACTAATTTGTGATCTTC+AGG 0.300971 7:+60986462 None:intergenic
CCATGGATTCATCAGTTTAT+CGG 0.305168 7:-60989897 MsaT037566.1:CDS
GTCCAAATTTGGTGAAGATA+TGG 0.313890 7:-60985410 MsaT037566.1:CDS
ACATTTCAACTCACAGTTAG+AGG 0.314147 7:+60988990 None:intergenic
AAATTCTAATTATGGTGATA+TGG 0.317057 7:-60988760 MsaT037566.1:CDS
TAGGTGAACCTGATTGCATT+TGG 0.330287 7:+60988652 None:intergenic
CTATTTGCATCAATAAGATT+TGG 0.330561 7:+60990305 None:intergenic
ATTAGTAGTTGGAGGGATTT+CGG 0.336955 7:-60985298 MsaT037566.1:intron
TGATCAACTAAGGCCTGGTT+TGG 0.343586 7:-60988889 MsaT037566.1:CDS
GTCGCCAACTGAGATGAATT+TGG 0.348706 7:+60986420 None:intergenic
AACGCAGAACCAATATCTTT+TGG 0.350058 7:-60988838 MsaT037566.1:CDS
TTCATGGCCAATGCACTTGC+TGG 0.354921 7:+60990380 None:intergenic
TGTGGAATATGGACTCTTTA+CGG 0.363207 7:-60986497 MsaT037566.1:CDS
TGTCTCAGTCAGTTGGTTCA+TGG 0.373309 7:-60990141 MsaT037566.1:intron
CCACCCAGTCCAGGATGATT+AGG 0.373675 7:+60990446 None:intergenic
CAGGATGATATAGATCGATT+TGG 0.381220 7:-60987414 MsaT037566.1:CDS
CAAAGGATGGTCAATCTAAC+AGG 0.384060 7:-60988695 MsaT037566.1:CDS
CAACATACCTTCAGAGTTCA+TGG 0.387665 7:+60990412 None:intergenic
GTGACAAATCTGTGCGGTTA+TGG 0.388414 7:-60986389 MsaT037566.1:CDS
AACAGGGAATTGATCAACTA+AGG 0.398629 7:-60988899 MsaT037566.1:intron
TGCGTTGTTAAATTAGGCTT+CGG 0.401986 7:+60988855 None:intergenic
AGGTGAACCTGATTGCATTT+GGG 0.402536 7:+60988653 None:intergenic
GCTATATTCTTGAACACAAT+AGG 0.403277 7:+60986214 None:intergenic
TGTGTTGATGACGGTGAATT+AGG 0.403713 7:+60987862 None:intergenic
CAAATATTGTTCACCGGCTC+AGG 0.410939 7:+60985468 None:intergenic
CAGTGCTGGAGAAGACAAAA+AGG 0.412474 7:-60987105 MsaT037566.1:intron
ATGAACTCTGAAGGTATGTT+GGG 0.414053 7:-60990410 MsaT037566.1:CDS
GTTTCCAGTACCAGTGCTAT+TGG 0.416797 7:+60987900 None:intergenic
GTCAGTATGAAGCCAACTTT+GGG 0.420214 7:-60991564 None:intergenic
TTGGCTGAGTGTGCTGCTAC+TGG 0.422243 7:-60984696 MsaT037566.1:CDS
CTCGTGTACCCGTGTATCTA+AGG 0.424307 7:-60986084 MsaT037566.1:intron
ATTTCCTCCCAAATGCAATC+AGG 0.425797 7:-60988660 MsaT037566.1:CDS
GGAGATGGTGGAAATCTCTA+TGG 0.430122 7:-60987339 MsaT037566.1:CDS
TGTCAGTATGAAGCCAACTT+TGG 0.431563 7:-60991565 None:intergenic
GACCTCAGGAGAATGCGTTC+AGG 0.438008 7:-60985382 MsaT037566.1:CDS
GGGTCAAGCTCAAAGTAATT+GGG 0.439675 7:-60991544 MsaT037566.1:CDS
ACTAATAAAGTTGCATAGCT+TGG 0.440621 7:+60985315 None:intergenic
TATGCAACTTTATTAGTAGT+TGG 0.442564 7:-60985309 MsaT037566.1:CDS
GTGATAGTGAAAATGAAATT+CGG 0.443112 7:-60986127 MsaT037566.1:CDS
AGCCGGTGAACAATATTTGT+TGG 0.446057 7:-60985464 MsaT037566.1:CDS
TTTGGCATCACAACAACAAC+AGG 0.447254 7:-60988820 MsaT037566.1:CDS
AATGAAACACCCTCATTCAA+TGG 0.448834 7:-60990342 MsaT037566.1:CDS
TCTTCTTAGGGTGGAAATCA+AGG 0.451351 7:+60986168 None:intergenic
GGAAAGTTGCTTGCCAGTGC+TGG 0.454974 7:-60987119 MsaT037566.1:CDS
GTGAGGTTTCAGCCTGGATC+AGG 0.455249 7:-60985965 MsaT037566.1:CDS
GCCGGTGAACAATATTTGTT+GGG 0.455398 7:-60985463 MsaT037566.1:CDS
GTTAGAGGCCACCCCTTCAA+TGG 0.455509 7:+60989005 None:intergenic
GTGGAAATCAAGGGACATAA+TGG 0.456387 7:+60986178 None:intergenic
GATCAACTAAGGCCTGGTTT+GGG 0.457801 7:-60988888 MsaT037566.1:CDS
TGACAAATCTGTGCGGTTAT+GGG 0.458834 7:-60986388 MsaT037566.1:CDS
TCCTGCATCATTCGATAGAA+AGG 0.466406 7:+60987359 None:intergenic
GCAAGCAACTGTCCTGATCC+AGG 0.471944 7:+60985953 None:intergenic
AGTTGGTTCATGGTAATTCT+GGG 0.472282 7:-60990131 MsaT037566.1:CDS
GTTCAGGAACTCAACTCTAC+TGG 0.475211 7:-60985366 MsaT037566.1:CDS
GTTGTGATGCCAAAAGATAT+TGG 0.477440 7:+60988829 None:intergenic
GCTGCCAATAGCACTGGTAC+TGG 0.478935 7:-60987904 MsaT037566.1:CDS
GCAACTTTATTAGTAGTTGG+AGG 0.479170 7:-60985306 MsaT037566.1:CDS
TGCGGTATTGCAGCAAATGC+AGG 0.483221 7:-60990100 MsaT037566.1:CDS
AATATTTGTTGGGATACTAC+TGG 0.484000 7:-60985453 MsaT037566.1:CDS
CTTTGAGCTTGACCCAAAGT+TGG 0.484960 7:+60991552 None:intergenic
TAGACCAAATTCATCTCAGT+TGG 0.486643 7:-60986424 MsaT037566.1:CDS
CCGATAAACTGATGAATCCA+TGG 0.487131 7:+60989897 None:intergenic
GTCCTTGGAGCTGTGGAACA+TGG 0.490801 7:-60984769 MsaT037566.1:CDS
CATGAACTCTGAAGGTATGT+TGG 0.492726 7:-60990411 MsaT037566.1:CDS
GCAGCAGCATCAACTGCAGC+AGG 0.494418 7:-60988058 MsaT037566.1:intron
AGATCCTAATCATCCTGGAC+TGG 0.494987 7:-60990450 MsaT037566.1:CDS
GAAATCACATTCTCATGTGC+AGG 0.497961 7:+60984723 None:intergenic
GGTCAATCTAACAGGAATGA+TGG 0.498321 7:-60988687 MsaT037566.1:CDS
CAACTTTATTAGTAGTTGGA+GGG 0.501616 7:-60985305 MsaT037566.1:CDS
TCTTATTGATGCAAATAGAA+TGG 0.502350 7:-60990300 MsaT037566.1:CDS
CTGGAGGATTTCTGTTTGAG+TGG 0.509340 7:-60991074 MsaT037566.1:CDS
CGAAGAGATCCTAATCATCC+TGG 0.509773 7:-60990455 MsaT037566.1:CDS
AAATCAGCAACAAGTCATCA+AGG 0.513351 7:-60990269 MsaT037566.1:CDS
AAATCTGGGTTAGGCAGTGC+AGG 0.515833 7:-60989856 MsaT037566.1:intron
TATTCTTGAACACAATAGGT+TGG 0.516797 7:+60986218 None:intergenic
ACAGGTCTTGGCACAGGCTC+AGG 0.517377 7:-60988802 MsaT037566.1:CDS
GGATACTACTGGAGATATGC+TGG 0.518775 7:-60985442 MsaT037566.1:CDS
ACAGAAGCAACAGGAGGACT+TGG 0.519265 7:-60987760 MsaT037566.1:CDS
ATGTATGGTACAGAAGCAAC+AGG 0.522786 7:-60987769 MsaT037566.1:CDS
GCAGCCACCAGCAAGTGCAT+TGG 0.523196 7:-60990387 MsaT037566.1:CDS
CATGGGAAAATTCTTTGGAC+TGG 0.526682 7:+60989915 None:intergenic
TTCAAAGAACCACCCAGTCC+AGG 0.526740 7:+60990437 None:intergenic
TCCTTTCTATCGAATGATGC+AGG 0.528349 7:-60987360 MsaT037566.1:CDS
CACCATAATTAGAATTTCCA+AGG 0.530895 7:+60988766 None:intergenic
ATCACAACAACAACAGGTCT+TGG 0.532144 7:-60988814 MsaT037566.1:CDS
ATGGCCAATGCACTTGCTGG+TGG 0.533520 7:+60990383 None:intergenic
TGAGAATGTGATTTCTGCGT+TGG 0.535664 7:-60984715 MsaT037566.1:CDS
AGTTACAAGTCTTCCATTGA+AGG 0.537705 7:-60989018 MsaT037566.1:CDS
CAGGCACAACAAAACAGCCT+TGG 0.542631 7:-60988783 MsaT037566.1:CDS
CAAAAGAGCATGATGATGTA+TGG 0.542686 7:-60987784 MsaT037566.1:CDS
CTATCGAATGATGCAGGAGA+TGG 0.543289 7:-60987354 MsaT037566.1:CDS
GAGACAATGATTGTTGTGAC+TGG 0.545286 7:+60988097 None:intergenic
GGAATTGATCAACTAAGGCC+TGG 0.546179 7:-60988894 MsaT037566.1:CDS
GATCCTAATCATCCTGGACT+GGG 0.546709 7:-60990449 MsaT037566.1:CDS
ACATCTGAATCCATTGAATG+AGG 0.547319 7:+60990332 None:intergenic
ATAGATCGATTTGGAGATGT+TGG 0.547428 7:-60987405 MsaT037566.1:CDS
TTCAGGAACTCAACTCTACT+GGG 0.548571 7:-60985365 MsaT037566.1:CDS
AACATACCTTCAGAGTTCAT+GGG 0.549284 7:+60990413 None:intergenic
GTGTGCAGTCCTTGGAGCTG+TGG 0.553456 7:-60984776 MsaT037566.1:intron
TCTGGAGCTGCCAATAGCAC+TGG 0.557212 7:-60987910 MsaT037566.1:CDS
TGAGTGTGCTGCTACTGGAA+TGG 0.557700 7:-60984691 MsaT037566.1:CDS
AAGATATGGAATTTGACCTC+AGG 0.557728 7:-60985396 MsaT037566.1:CDS
CTTTACAGAGTTGTCACGAC+TGG 0.558560 7:+60984659 None:intergenic
CGATAAACTGATGAATCCAT+GGG 0.559912 7:+60989898 None:intergenic
GTTACAAGTCTTCCATTGAA+GGG 0.560570 7:-60989017 MsaT037566.1:CDS
TTACAATCGAAATCTGGGTT+AGG 0.560892 7:-60989865 MsaT037566.1:CDS
CAAGTCTTCCATTGAAGGGG+TGG 0.561593 7:-60989013 MsaT037566.1:CDS
TTGAACCCCATGAACTCTGA+AGG 0.565456 7:-60990419 MsaT037566.1:CDS
AAAAGTGACAAGAAGTAACT+TGG 0.565623 7:+60987150 None:intergenic
TTTGCAGGGTCATCCTGAGC+CGG 0.566190 7:-60985481 MsaT037566.1:intron
GTAAAGAGTCCATATTCCAC+AGG 0.566610 7:+60986499 None:intergenic
TGTTGTCATACAGATGAAGA+TGG 0.570938 7:-60988121 MsaT037566.1:intron
GAGGATTTCTGTTTGAGTGG+TGG 0.572454 7:-60991071 MsaT037566.1:CDS
AGAGTTGTCACGACTGGCAG+AGG 0.573691 7:+60984665 None:intergenic
CATCTGAATCCATTGAATGA+GGG 0.576618 7:+60990333 None:intergenic
TCCCAACAAATATTGTTCAC+CGG 0.579006 7:+60985462 None:intergenic
TTGATAGCAATTGATGCACC+TGG 0.581055 7:-60991093 MsaT037566.1:intron
CAGACAAATTTACTCACTCC+AGG 0.587469 7:-60985885 MsaT037566.1:intron
ACTGGTACTGGAAACACAGC+TGG 0.587738 7:-60987892 MsaT037566.1:CDS
CTTCTTAGGGTGGAAATCAA+GGG 0.588390 7:+60986169 None:intergenic
CCGTCATCAACACACACACC+TGG 0.589445 7:-60987853 MsaT037566.1:CDS
ATTGTGTTCAAGAATATAGC+GGG 0.590018 7:-60986211 MsaT037566.1:CDS
GTGACAACTCTGTAAAGTTG+TGG 0.593245 7:-60984653 MsaT037566.1:CDS
GATGAATATATCCCAAAAGA+CGG 0.596758 7:+60991047 None:intergenic
TATTGCAGCAAATGCAGGCA+CGG 0.597994 7:-60990095 MsaT037566.1:CDS
AATGCAATCAGGTTCACCTA+AGG 0.600370 7:-60988649 MsaT037566.1:intron
ATCAAAGGAGAAGTTAACAT+TGG 0.600466 7:-60989940 MsaT037566.1:CDS
AGCCATGTTCCACAGCTCCA+AGG 0.601941 7:+60984767 None:intergenic
GCAAAAGTGAGGTTTCAGCC+TGG 0.602949 7:-60985971 MsaT037566.1:CDS
TCGAATGATGCAGGAGATGG+TGG 0.603645 7:-60987351 MsaT037566.1:CDS
CACTCAAACAGAAATCCTCC+AGG 0.604315 7:+60991075 None:intergenic
TATGCAGCTAAATTCAGCAA+AGG 0.604600 7:+60987184 None:intergenic
TGAACCTGATTGCATTTGGG+AGG 0.613490 7:+60988656 None:intergenic
TCGAGGTAGCTTAAATGCAA+AGG 0.613666 7:-60988712 MsaT037566.1:CDS
CTTAGATACACGGGTACACG+AGG 0.614445 7:+60986085 None:intergenic
ACAACAACAGGTCTTGGCAC+AGG 0.616147 7:-60988808 MsaT037566.1:CDS
TTACAAGTCTTCCATTGAAG+GGG 0.616668 7:-60989016 MsaT037566.1:CDS
TTGACCAACCTTAGATACAC+GGG 0.620122 7:+60986076 None:intergenic
AATCAGCAACAAGTCATCAA+GGG 0.625746 7:-60990268 MsaT037566.1:intron
TTTGACCAACCTTAGATACA+CGG 0.626725 7:+60986075 None:intergenic
GGAACTTGTACACCCAAACC+AGG 0.627589 7:+60988876 None:intergenic
CCTAATCATCCTGGACTGGG+TGG 0.629932 7:-60990446 MsaT037566.1:CDS
TGGTGCTTTAGATGATAATG+TGG 0.631026 7:-60987385 MsaT037566.1:CDS
GCTGTATTCAATACATCACC+AGG 0.633261 7:+60987835 None:intergenic
GGTAGCTTAAATGCAAAGGA+TGG 0.633429 7:-60988708 MsaT037566.1:CDS
TTCCTGAACGCATTCTCCTG+AGG 0.634159 7:+60985380 None:intergenic
AAGGTTTCAACTGATCCAGT+AGG 0.634552 7:-60991318 MsaT037566.1:intron
AGGGAGGTAATGCAAAAGTG+AGG 0.639313 7:-60985982 MsaT037566.1:CDS
CCGCACAGATTTGTCACATG+AGG 0.645521 7:+60986395 None:intergenic
AGACAATGATTGTTGTGACT+GGG 0.645930 7:+60988098 None:intergenic
ACATACCTTCAGAGTTCATG+GGG 0.646193 7:+60990414 None:intergenic
TAATTTGTGATCTTCAGGAG+TGG 0.651459 7:+60986467 None:intergenic
AATAAAACAAAGCCCAGCTG+AGG 0.653660 7:-60987313 MsaT037566.1:CDS
AGACTTTCTGGTCTTCCTCG+AGG 0.655968 7:-60988729 MsaT037566.1:CDS
AATTTAGCTGCATAAGAACA+AGG 0.658156 7:-60987175 MsaT037566.1:CDS
CCAGGTGTGTGTGTTGATGA+CGG 0.659988 7:+60987853 None:intergenic
AACAAATGAGAAGCATTCTG+AGG 0.662829 7:-60991019 MsaT037566.1:CDS
GGCTGCTGCATCTTACATTG+AGG 0.668511 7:-60990998 MsaT037566.1:intron
TTGGTAGACACAGCAAACAA+AGG 0.669557 7:-60990540 MsaT037566.1:intron
TGAACTCTGAAGGTATGTTG+GGG 0.670256 7:-60990409 MsaT037566.1:CDS
TATTGTGTTCAAGAATATAG+CGG 0.672769 7:-60986212 MsaT037566.1:CDS
ATAGCAATTGATGCACCTGG+AGG 0.686315 7:-60991090 MsaT037566.1:intron
TAAAGAGTCCATATTCCACA+GGG 0.698758 7:+60986500 None:intergenic
TAATTCTGGGAATATGTCTG+CGG 0.708677 7:-60990118 MsaT037566.1:CDS
GGAAGACCAGAAAGTCTGCG+TGG 0.711585 7:+60988735 None:intergenic
TTTGCATTTAAGCTACCTCG+AGG 0.727027 7:+60988714 None:intergenic
GCATGTATCAGGTTACCCTG+TGG 0.729250 7:-60986515 MsaT037566.1:intron
TATGGTACAGAAGCAACAGG+AGG 0.736146 7:-60987766 MsaT037566.1:CDS
GATGACGGTGAATTAGGTGA+AGG 0.756690 7:+60987868 None:intergenic
CCTCATGTGACAAATCTGTG+CGG 0.774368 7:-60986395 MsaT037566.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 60984647 60991580 60984647 ID=MsaG037566
Chr7 mRNA 60984647 60991580 60984647 ID=MsaT037566.1;Parent=MsaG037566
Chr7 exon 60984647 60984790 60984647 ID=MsaT037566.1.exon17;Parent=MsaT037566.1
Chr7 CDS 60984647 60984790 60984647 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60985299 60985496 60985299 ID=MsaT037566.1.exon16;Parent=MsaT037566.1
Chr7 CDS 60985299 60985496 60985299 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60985886 60986002 60985886 ID=MsaT037566.1.exon15;Parent=MsaT037566.1
Chr7 CDS 60985886 60986002 60985886 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60986085 60986240 60986085 ID=MsaT037566.1.exon14;Parent=MsaT037566.1
Chr7 CDS 60986085 60986240 60986085 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60986377 60986526 60986377 ID=MsaT037566.1.exon13;Parent=MsaT037566.1
Chr7 CDS 60986377 60986526 60986377 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60987106 60987212 60987106 ID=MsaT037566.1.exon12;Parent=MsaT037566.1
Chr7 CDS 60987106 60987212 60987106 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60987292 60987433 60987292 ID=MsaT037566.1.exon11;Parent=MsaT037566.1
Chr7 CDS 60987292 60987433 60987292 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60987741 60987962 60987741 ID=MsaT037566.1.exon10;Parent=MsaT037566.1
Chr7 CDS 60987741 60987962 60987741 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60988059 60988130 60988059 ID=MsaT037566.1.exon9;Parent=MsaT037566.1
Chr7 CDS 60988059 60988130 60988059 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60988650 60988916 60988650 ID=MsaT037566.1.exon8;Parent=MsaT037566.1
Chr7 CDS 60988650 60988916 60988650 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60989004 60989053 60989004 ID=MsaT037566.1.exon7;Parent=MsaT037566.1
Chr7 CDS 60989004 60989053 60989004 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60989857 60989965 60989857 ID=MsaT037566.1.exon6;Parent=MsaT037566.1
Chr7 CDS 60989857 60989965 60989857 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60990080 60990155 60990080 ID=MsaT037566.1.exon5;Parent=MsaT037566.1
Chr7 CDS 60990080 60990155 60990080 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60990269 60990555 60990269 ID=MsaT037566.1.exon4;Parent=MsaT037566.1
Chr7 CDS 60990269 60990555 60990269 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60990999 60991108 60990999 ID=MsaT037566.1.exon3;Parent=MsaT037566.1
Chr7 CDS 60990999 60991108 60990999 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60991319 60991416 60991319 ID=MsaT037566.1.exon2;Parent=MsaT037566.1
Chr7 CDS 60991319 60991416 60991319 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Chr7 exon 60991531 60991580 60991531 ID=MsaT037566.1.exon1;Parent=MsaT037566.1
Chr7 CDS 60991531 60991580 60991531 ID=cds.MsaT037566.1;Parent=MsaT037566.1
Gene Sequence

>MsaT037566.1

ATGAAGCCAACTTTGGGTCAAGCTCAAAGTAATTGGGAAGCTGACAAAATGCTTGATGTTTATATTCATGATTATTTTCTCAAAAGAAGGTTACACAACACTGCGAAAGCTTTTATGGCAGAAGGAAAGGTTTCAACTGATCCAGTAGCAATTGATGCACCTGGAGGATTTCTGTTTGAGTGGTGGTCCGTCTTTTGGGATATATTCATCTCGAGAACAAATGAGAAGCATTCTGAGGCTGCTGCATCTTACATTGAGACACAGCAAACAAAGGCGAGAGAACAGCTTCAAATGCAGCAATTGCAACTGATGCAACAGCGCAATGCGCAACTGCAGCGAAGAGATCCTAATCATCCTGGACTGGGTGGTTCTTTGAACCCCATGAACTCTGAAGGTATGTTGGGGCAGCCACCAGCAAGTGCATTGGCCATGAAAATGTATGAAGAACGAATGAAACACCCTCATTCAATGGATTCAGATGTATCTCCAAATCTTATTGATGCAAATAGAATGGCTCTTCTCAAATCAGCAACAAGTCATCAAGGTCAGTTGGTTCATGGTAATTCTGGGAATATGTCTGCGGTATTGCAGCAAATGCAGGCACGGTCTGCTTTGAACAACGACATCAAAGGAGAAGTTAACATTGGTGCCAGTCCAAAGAATTTTCCCATGGATTCATCAGTTTATCGGCAATCAATTTTACAATCGAAATCTGGGTTAGGCAGTGCAGGACTAAATCAAGGAGTTACAAGTCTTCCATTGAAGGGGTGGCCTCTAACTGGAATTGATCAACTAAGGCCTGGTTTGGGTGTACAAGTTCCGAAGCCTAATTTAACAACGCAGAACCAATATCTTTTGGCATCACAACAACAACAGGTCTTGGCACAGGCTCAGGCACAACAAAACAGCCTTGGAAATTCTAATTATGGTGATATGGATCCACGCAGACTTTCTGGTCTTCCTCGAGGTAGCTTAAATGCAAAGGATGGTCAATCTAACAGGAATGATGGATCTATTTCCTCCCAAATGCAATCAGGTTCACCTAAGATGAAGATGGCCCAGTCACAACAATCATTGTCTCAGCAGCAAGAGCAATTGCAGCAGCATCAACTGCAGCAGAGTAACAGAAAAAGAAAGCAAGCTTCTTCTTCTGGAGCTGCCAATAGCACTGGTACTGGAAACACAGCTGGTCCTTCACCTAATTCACCGTCATCAACACACACACCTGGTGATGTATTGAATACAGCAAGCAGCATGCAACATGTTAACAATGTGCAAAAGAGCATGATGATGTATGGTACAGAAGCAACAGGAGGACTTGGATCATCTTCAAATTTACTTGATGATATAGATCGATTTGGAGATGTTGGTGCTTTAGATGATAATGTGGAGTCCTTTCTATCGAATGATGCAGGAGATGGTGGAAATCTCTATGGAACAATAAAACAAAGCCCAGCTGAGGAACAAAAAGAATCTTCAAAAACTTTCACCTTTGCTGAATTTAGCTGCATAAGAACAAGGAACCAAGTTACTTCTTGTCACTTTTCATCAGATGGAAAGTTGCTTGCCAGTGCTGGAGAAGACAAAAAGGTTACCCTGTGGAATATGGACTCTTTACGGACAGAGACCACTCCTGAAGATCACAAATTAGTTATTACAGATGTTCGTTTTAGACCAAATTCATCTCAGTTGGCGACAGCCTCATGTGACAAATCTGTGCGGTTATGGGACGCAGCCAATCCAACCTATTGTGTTCAAGAATATAGCGGGCACAGTTCAGCCATTATGTCCCTTGATTTCCACCCTAAGAAGACTGATTTTTTCTGCTTCTGTGATAGTGAAAATGAAATTCGGTACTATAACATTAGTTCATCCTCGTGTACCCGTGTATCTAAGGGAGGTAATGCAAAAGTGAGGTTTCAGCCTGGATCAGGACAGTTGCTTGCTGCAGCTTCTGATAAAGTAGTGTCTATTTTTGATGTTGAAACTGACAGACAAATTTACTCACTCCAGGGTCATCCTGAGCCGGTGAACAATATTTGTTGGGATACTACTGGAGATATGCTGGCATCTATTAGTCCAAATTTGGTGAAGATATGGAATTTGACCTCAGGAGAATGCGTTCAGGAACTCAACTCTACTGGGAACCAGTTTTATTCTGGTCTTTTTCATCCAAGCTATGCAACTTTATTAGTAGTTGGAGGGATTTCGTCCTTGGAGCTGTGGAACATGGCTGAGAACAAAAGCATGACAATTCCTGCACATGAGAATGTGATTTCTGCGTTGGCTGAGTGTGCTGCTACTGGAATGGTTGCCTCTGCCAGTCGTGACAACTCTGTAAAGTTGTGGAAATAA
Protein sequence

>MsaT037566.1

MKPTLGQAQSNWEADKMLDVYIHDYFLKRRLHNTAKAFMAEGKVSTDPVAIDAPGGFLFEWWSVFWDIFISRTNEKHSEAAASYIETQQTKAREQLQMQQLQLMQQRNAQLQRRDPNHPGLGGSLNPMNSEGMLGQPPASALAMKMYEERMKHPHSMDSDVSPNLIDANRMALLKSATSHQGQLVHGNSGNMSAVLQQMQARSALNNDIKGEVNIGASPKNFPMDSSVYRQSILQSKSGLGSAGLNQGVTSLPLKGWPLTGIDQLRPGLGVQVPKPNLTTQNQYLLASQQQQVLAQAQAQQNSLGNSNYGDMDPRRLSGLPRGSLNAKDGQSNRNDGSISSQMQSGSPKMKMAQSQQSLSQQQEQLQQHQLQQSNRKRKQASSSGAANSTGTGNTAGPSPNSPSSTHTPGDVLNTASSMQHVNNVQKSMMMYGTEATGGLGSSSNLLDDIDRFGDVGALDDNVESFLSNDAGDGGNLYGTIKQSPAEEQKESSKTFTFAEFSCIRTRNQVTSCHFSSDGKLLASAGEDKKVTLWNMDSLRTETTPEDHKLVITDVRFRPNSSQLATASCDKSVRLWDAANPTYCVQEYSGHSSAIMSLDFHPKKTDFFCFCDSENEIRYYNISSSSCTRVSKGGNAKVRFQPGSGQLLAAASDKVVSIFDVETDRQIYSLQGHPEPVNNICWDTTGDMLASISPNLVKIWNLTSGECVQELNSTGNQFYSGLFHPSYATLLVVGGISSLELWNMAENKSMTIPAHENVISALAECAATGMVASASRDNSVKLWK*