Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037604 | AES79212.1 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 5.33e-52 | 167 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037604 | sp|Q8GW32|PRE6_ARATH | 47.297 | 74 | 39 | 0 | 16 | 89 | 21 | 94 | 1.58e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037604 | tr|G7L2D1|G7L2D1_MEDTR | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 2.55e-52 | 167 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG037604 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037604 | MtrunA17_Chr7g0237771 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 4.90e-56 | 167 |
| MsaG037604 | MtrunA17_Chr8g0362131 | 50.746 | 67 | 32 | 1 | 10 | 76 | 15 | 80 | 2.98e-17 | 69.7 |
| MsaG037604 | MtrunA17_Chr3g0093001 | 50.794 | 63 | 31 | 0 | 12 | 74 | 17 | 79 | 4.40e-16 | 67.0 |
| MsaG037604 | MtrunA17_Chr5g0416431 | 46.970 | 66 | 35 | 0 | 11 | 76 | 16 | 81 | 5.49e-16 | 66.6 |
| MsaG037604 | MtrunA17_Chr6g0453041 | 49.206 | 63 | 32 | 0 | 12 | 74 | 15 | 77 | 1.67e-14 | 62.8 |
| MsaG037604 | MtrunA17_Chr7g0217141 | 43.836 | 73 | 41 | 0 | 2 | 74 | 6 | 78 | 2.11e-14 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG037604 | AT1G26945.1 | 47.297 | 74 | 39 | 0 | 16 | 89 | 21 | 94 | 1.60e-17 | 70.9 |
| MsaG037604 | AT3G47710.1 | 53.521 | 71 | 32 | 1 | 5 | 74 | 9 | 79 | 6.29e-17 | 69.3 |
| MsaG037604 | AT3G28857.1 | 47.436 | 78 | 37 | 2 | 1 | 74 | 1 | 78 | 1.19e-15 | 66.2 |
| MsaG037604 | AT5G39860.1 | 50.769 | 65 | 31 | 1 | 11 | 74 | 14 | 78 | 6.18e-15 | 64.3 |
| MsaG037604 | AT5G15160.1 | 53.226 | 62 | 28 | 1 | 16 | 76 | 20 | 81 | 1.10e-13 | 61.2 |
| MsaG037604 | AT1G74500.1 | 48.438 | 64 | 32 | 1 | 12 | 74 | 17 | 80 | 1.14e-13 | 61.2 |
Find 17 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAACATTGGATCATATT+AGG | 0.284722 | 7:-61509571 | MsaT037604.1:CDS |
| ATACCCTTGAGTTGTTTCTT+TGG | 0.331022 | 7:+61511088 | None:intergenic |
| CAATGTTTCCTGCAAGATCT+TGG | 0.360132 | 7:+61509583 | None:intergenic |
| TTGAATCTCTTGAGTTTCTC+TGG | 0.365643 | 7:+61511175 | None:intergenic |
| TGAGAGGCTAACACAATTGA+TGG | 0.413964 | 7:-61509519 | MsaT037604.1:CDS |
| ATCAGCCTCCAAGATCTTGC+AGG | 0.440449 | 7:-61509591 | MsaT037604.1:CDS |
| CAAGATCTTGCAGGAAACAT+TGG | 0.497669 | 7:-61509582 | MsaT037604.1:CDS |
| TGTTTCCTGCAAGATCTTGG+AGG | 0.509912 | 7:+61509586 | None:intergenic |
| TAAGAAAAGAGAGTCATGTC+TGG | 0.537278 | 7:-61511198 | None:intergenic |
| TATTAGGAGGCTTCAGAAAG+AGG | 0.545892 | 7:-61509555 | MsaT037604.1:CDS |
| TTGTTTCTTTGGTTCAGCTG+AGG | 0.545919 | 7:+61511099 | None:intergenic |
| AAACATTGGATCATATTAGG+AGG | 0.601405 | 7:-61509568 | MsaT037604.1:CDS |
| TAGGAGGCTTCAGAAAGAGG+TGG | 0.622036 | 7:-61509552 | MsaT037604.1:CDS |
| TGAACCAAAGAAACAACTCA+AGG | 0.632348 | 7:-61511092 | MsaT037604.1:CDS |
| AGGTGGAAGATCTCAGTGAG+AGG | 0.651028 | 7:-61509535 | MsaT037604.1:CDS |
| AGATTAATGATCTTGTGTCA+AGG | 0.656005 | 7:-61511137 | MsaT037604.1:CDS |
| GAACCAAAGAAACAACTCAA+GGG | 0.668866 | 7:-61511091 | MsaT037604.1:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 61509460 | 61511205 | 61509460 | ID=MsaG037604 |
| Chr7 | mRNA | 61509460 | 61511205 | 61509460 | ID=MsaT037604.1;Parent=MsaG037604 |
| Chr7 | exon | 61509460 | 61509615 | 61509460 | ID=MsaT037604.1.exon2;Parent=MsaT037604.1 |
| Chr7 | CDS | 61509460 | 61509615 | 61509460 | ID=cds.MsaT037604.1;Parent=MsaT037604.1 |
| Chr7 | exon | 61511092 | 61511205 | 61511092 | ID=MsaT037604.1.exon1;Parent=MsaT037604.1 |
| Chr7 | CDS | 61511092 | 61511205 | 61511092 | ID=cds.MsaT037604.1;Parent=MsaT037604.1 |
| Gene Sequence |
| Protein sequence |
