Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG037635 | KAG5243843.1 | 79.755 | 163 | 33 | 0 | 5 | 167 | 4 | 166 | 7.17e-88 | 265 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG037635 | sp|Q9LZD7|MED7B_ARATH | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.71e-80 | 238 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG037635 | tr|A0A059DIJ1|A0A059DIJ1_EUCGR | 80.000 | 165 | 33 | 0 | 5 | 169 | 4 | 168 | 4.30e-85 | 256 |
Gene ID | Type | Classification |
---|---|---|
MsaG037635 | TR | MED7 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000583 | MsaG037635 | 0.811390 | 1.229829e-50 | 2.172738e-48 |
MsaG002731 | MsaG037635 | 0.829655 | 8.184758e-55 | 2.345474e-52 |
MsaG003938 | MsaG037635 | 0.805083 | 2.676915e-49 | 4.066204e-47 |
MsaG004227 | MsaG037635 | 0.820234 | 1.338682e-52 | 2.962709e-50 |
MsaG004367 | MsaG037635 | 0.834764 | 4.519038e-56 | 1.503113e-53 |
MsaG004465 | MsaG037635 | 0.802386 | 9.656423e-49 | 1.378190e-46 |
MsaG004782 | MsaG037635 | 0.814789 | 2.227039e-51 | 4.282223e-49 |
MsaG005282 | MsaG037635 | 0.855222 | 1.410016e-61 | 9.182372e-59 |
MsaG005288 | MsaG037635 | 0.807196 | 9.659570e-50 | 1.541973e-47 |
MsaG005664 | MsaG037635 | 0.823918 | 1.891327e-53 | 4.619278e-51 |
MsaG006425 | MsaG037635 | 0.801907 | 1.210299e-48 | 1.708570e-46 |
MsaG006586 | MsaG037635 | 0.803223 | 6.499223e-49 | 9.455822e-47 |
MsaG006743 | MsaG037635 | 0.827434 | 2.799120e-54 | 7.533817e-52 |
MsaG007094 | MsaG037635 | 0.835086 | 3.752649e-56 | 1.260235e-53 |
MsaG007665 | MsaG037635 | 0.811544 | 1.138746e-50 | 2.019443e-48 |
MsaG007759 | MsaG037635 | 0.801514 | 1.456282e-48 | 2.037469e-46 |
MsaG009172 | MsaG037635 | 0.805405 | 2.293360e-49 | 3.509729e-47 |
MsaG009495 | MsaG037635 | 0.824339 | 1.508182e-53 | 3.726137e-51 |
MsaG010314 | MsaG037635 | 0.811086 | 1.430494e-50 | 2.508472e-48 |
MsaG010621 | MsaG037635 | 0.824446 | 1.423741e-53 | 3.527608e-51 |
MsaG011133 | MsaG037635 | 0.833615 | 8.740157e-56 | 2.809498e-53 |
MsaG044793 | MsaG037635 | 0.813625 | 4.013272e-51 | 7.494658e-49 |
MsaG045861 | MsaG037635 | 0.840669 | 1.399942e-57 | 5.581040e-55 |
MsaG045883 | MsaG037635 | 0.801064 | 1.798276e-48 | 2.490386e-46 |
MsaG046532 | MsaG037635 | 0.809577 | 3.016205e-50 | 5.098706e-48 |
MsaG002505 | MsaG037635 | 0.802424 | 9.487731e-49 | 1.355277e-46 |
MsaG003580 | MsaG037635 | 0.807940 | 6.726881e-50 | 1.093152e-47 |
MsaG013255 | MsaG037635 | 0.819335 | 2.143572e-52 | 4.632746e-50 |
MsaG013398 | MsaG037635 | 0.865747 | 9.450260e-65 | 9.198727e-62 |
MsaG025840 | MsaG037635 | 0.803532 | 5.612035e-49 | 8.223609e-47 |
MsaG026617 | MsaG037635 | 0.819901 | 1.593902e-52 | 3.496354e-50 |
MsaG027196 | MsaG037635 | 0.810448 | 1.962127e-50 | 3.387672e-48 |
MsaG034035 | MsaG037635 | 0.801606 | 1.394686e-48 | 1.955395e-46 |
MsaG031458 | MsaG037635 | 0.819071 | 2.459526e-52 | 5.278684e-50 |
MsaG032719 | MsaG037635 | 0.842676 | 4.161669e-58 | 1.768617e-55 |
MsaG030868 | MsaG037635 | 0.807933 | 6.748968e-50 | 1.096568e-47 |
MsaG034266 | MsaG037635 | 0.825240 | 9.266171e-54 | 2.346294e-51 |
MsaG031897 | MsaG037635 | 0.826891 | 3.770591e-54 | 9.995554e-52 |
MsaG037375 | MsaG037635 | 0.822371 | 4.325638e-53 | 1.013201e-50 |
MsaG037635 | MsaG037000 | 0.806165 | 1.590841e-49 | 2.478440e-47 |
MsaG037635 | MsaG037387 | 0.829306 | 9.942346e-55 | 2.820609e-52 |
MsaG037635 | MsaG036656 | 0.817849 | 4.640946e-52 | 9.648921e-50 |
MsaG013177 | MsaG037635 | 0.828153 | 1.883026e-54 | 5.171622e-52 |
MsaG014560 | MsaG037635 | 0.812535 | 6.944474e-51 | 1.262012e-48 |
MsaG015329 | MsaG037635 | 0.823542 | 2.313737e-53 | 5.593799e-51 |
MsaG015347 | MsaG037635 | 0.804149 | 4.183711e-49 | 6.218937e-47 |
MsaG016474 | MsaG037635 | 0.819310 | 2.171325e-52 | 4.689508e-50 |
MsaG016707 | MsaG037635 | 0.804972 | 2.823412e-49 | 4.277652e-47 |
MsaG016972 | MsaG037635 | 0.800335 | 2.529094e-48 | 3.445367e-46 |
MsaG017085 | MsaG037635 | 0.827863 | 2.210367e-54 | 6.021298e-52 |
MsaG017267 | MsaG037635 | 0.802796 | 7.955153e-49 | 1.146041e-46 |
MsaG018669 | MsaG037635 | 0.804663 | 3.273766e-49 | 4.924402e-47 |
MsaG018847 | MsaG037635 | 0.820823 | 9.820925e-53 | 2.207412e-50 |
MsaG018920 | MsaG037635 | 0.800755 | 2.077934e-48 | 2.857874e-46 |
MsaG019019 | MsaG037635 | 0.817001 | 7.189861e-52 | 1.462582e-49 |
MsaG019292 | MsaG037635 | 0.803468 | 5.784864e-49 | 8.464295e-47 |
MsaG019937 | MsaG037635 | 0.812629 | 6.623359e-51 | 1.206435e-48 |
MsaG019939 | MsaG037635 | 0.805754 | 1.939620e-49 | 2.992637e-47 |
MsaG020075 | MsaG037635 | 0.800434 | 2.414178e-48 | 3.296308e-46 |
MsaG020567 | MsaG037635 | 0.835218 | 3.475194e-56 | 1.171648e-53 |
MsaG020667 | MsaG037635 | 0.825961 | 6.265346e-54 | 1.618561e-51 |
MsaG021001 | MsaG037635 | 0.803567 | 5.520797e-49 | 8.096232e-47 |
MsaG021418 | MsaG037635 | 0.833565 | 8.993714e-56 | 2.886675e-53 |
MsaG022472 | MsaG037635 | 0.814080 | 3.189817e-51 | 6.025161e-49 |
MsaG022820 | MsaG037635 | 0.819569 | 1.896956e-52 | 4.124594e-50 |
MsaG022824 | MsaG037635 | 0.807166 | 9.799651e-50 | 1.563253e-47 |
MsaG023442 | MsaG037635 | 0.817551 | 5.412422e-52 | 1.116718e-49 |
MsaG024150 | MsaG037635 | 0.822398 | 4.264318e-53 | 9.995495e-51 |
MsaG024152 | MsaG037635 | 0.812213 | 8.158839e-51 | 1.470803e-48 |
MsaG024586 | MsaG037635 | 0.820350 | 1.259569e-52 | 2.796135e-50 |
MsaG025262 | MsaG037635 | 0.850438 | 3.236120e-60 | 1.780405e-57 |
MsaG025451 | MsaG037635 | 0.800547 | 2.290090e-48 | 3.134865e-46 |
MsaG025797 | MsaG037635 | 0.808093 | 6.242816e-50 | 1.018217e-47 |
MsaG026926 | MsaG037635 | 0.813891 | 3.510726e-51 | 6.599977e-49 |
MsaG027046 | MsaG037635 | 0.805368 | 2.334921e-49 | 3.570216e-47 |
MsaG027329 | MsaG037635 | 0.817296 | 6.174358e-52 | 1.265616e-49 |
MsaG027970 | MsaG037635 | 0.815406 | 1.626887e-51 | 3.177477e-49 |
MsaG028348 | MsaG037635 | 0.813428 | 4.433019e-51 | 8.237790e-49 |
MsaG028931 | MsaG037635 | 0.820689 | 1.053823e-52 | 2.360258e-50 |
MsaG030080 | MsaG037635 | 0.834762 | 4.522368e-56 | 1.504164e-53 |
MsaG033125 | MsaG037635 | 0.821642 | 6.371587e-53 | 1.463601e-50 |
MsaG034999 | MsaG037635 | 0.819541 | 1.925040e-52 | 4.182578e-50 |
MsaG035300 | MsaG037635 | 0.816516 | 9.221306e-52 | 1.852505e-49 |
MsaG036128 | MsaG037635 | 0.803524 | 5.634162e-49 | 8.254418e-47 |
MsaG036529 | MsaG037635 | 0.813085 | 5.268230e-51 | 9.706015e-49 |
MsaG036699 | MsaG037635 | 0.806885 | 1.122917e-49 | 1.779419e-47 |
MsaG039591 | MsaG037635 | 0.808564 | 4.958843e-50 | 8.180097e-48 |
MsaG041706 | MsaG037635 | 0.814586 | 2.468874e-51 | 4.722807e-49 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG037635 | MtrunA17_Chr6g0478701 | 86.061 | 165 | 23 | 0 | 5 | 169 | 4 | 168 | 1.06e-85 | 249 |
MsaG037635 | MtrunA17_Chr2g0326811 | 85.455 | 165 | 24 | 0 | 5 | 169 | 4 | 168 | 4.51e-85 | 247 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG037635 | AT5G03500.3 | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.74e-81 | 238 |
MsaG037635 | AT5G03500.4 | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.74e-81 | 238 |
MsaG037635 | AT5G03500.2 | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.74e-81 | 238 |
MsaG037635 | AT5G03500.5 | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.74e-81 | 238 |
MsaG037635 | AT5G03500.7 | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.74e-81 | 238 |
MsaG037635 | AT5G03500.1 | 74.847 | 163 | 41 | 0 | 5 | 167 | 4 | 166 | 1.74e-81 | 238 |
MsaG037635 | AT5G03500.6 | 74.847 | 163 | 41 | 0 | 5 | 167 | 14 | 176 | 2.92e-81 | 238 |
MsaG037635 | AT5G03220.1 | 75.460 | 163 | 40 | 0 | 5 | 167 | 4 | 166 | 1.46e-74 | 221 |
Find 40 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ATCAACATTGATTCATATTA+TGG | 0.195694 | 7:-62035044 | MsaT037635.1:CDS |
TAGACTTTACAAATATTATT+TGG | 0.223723 | 7:-62038121 | MsaT037635.1:CDS |
AGAACTGCAACTACACATTT+TGG | 0.235567 | 7:-62035304 | MsaT037635.1:CDS |
CAAGGGACTTGCATTTGTTT+TGG | 0.271921 | 7:-62038060 | MsaT037635.1:CDS |
GCAACACTCGATGACCATTA+AGG | 0.341261 | 7:-62034100 | None:intergenic |
TGAATTGGCGGTGGAGGCTC+TGG | 0.388357 | 7:+62038081 | None:intergenic |
TATTTGTAAAGTCTATAATA+AGG | 0.399185 | 7:+62038129 | None:intergenic |
AAATTCATTGCATCCTCATC+AGG | 0.403386 | 7:-62035190 | MsaT037635.1:intron |
CTATAATAAGGTGGTGGTGG+TGG | 0.415430 | 7:+62038141 | None:intergenic |
CAAATGCAAGTCCCTTGAAT+TGG | 0.416060 | 7:+62038066 | None:intergenic |
GATAGAGCTGGCGCACCCCT+TGG | 0.428730 | 7:+62036185 | None:intergenic |
TGTCTTCTACGGCTTGTTTG+CGG | 0.449632 | 7:+62035005 | None:intergenic |
GTCCCTTGAATTGGCGGTGG+AGG | 0.451047 | 7:+62038075 | None:intergenic |
GAGCCTCCACCGCCAATTCA+AGG | 0.453043 | 7:-62038078 | MsaT037635.1:CDS |
CGTACCAACATTCGGCCCCT+TGG | 0.453104 | 7:+62036162 | None:intergenic |
ATACCTCTTTATGTCTTCTA+CGG | 0.455805 | 7:+62034994 | None:intergenic |
AAAGATAGCGTACCAACATT+CGG | 0.494584 | 7:+62036154 | None:intergenic |
GTACCAACATTCGGCCCCTT+GGG | 0.495917 | 7:+62036163 | None:intergenic |
TATAATAAGGTGGTGGTGGT+GGG | 0.505040 | 7:+62038142 | None:intergenic |
CAGCGACGTTCTACTAAGCT+TGG | 0.509261 | 7:-62036212 | MsaT037635.1:CDS |
ATGCAAGTCCCTTGAATTGG+CGG | 0.513484 | 7:+62038069 | None:intergenic |
AGTCTATAATAAGGTGGTGG+TGG | 0.525802 | 7:+62038138 | None:intergenic |
ACTCTTCTTGCATACTGTGA+TGG | 0.527495 | 7:+62035255 | None:intergenic |
AAGCCGTAGAAGACATAAAG+AGG | 0.531060 | 7:-62034997 | MsaT037635.1:intron |
TCGGCCCCTTGGGATAGAGC+TGG | 0.544781 | 7:+62036173 | None:intergenic |
TATCCCAAGGGGCCGAATGT+TGG | 0.548924 | 7:-62036166 | MsaT037635.1:intron |
AGCCTCCACCGCCAATTCAA+GGG | 0.558105 | 7:-62038077 | MsaT037635.1:CDS |
CAAGCTTAGTAGAACGTCGC+TGG | 0.558886 | 7:+62036213 | None:intergenic |
CAAGTCCCTTGAATTGGCGG+TGG | 0.561181 | 7:+62038072 | None:intergenic |
GTGCGCCAGCTCTATCCCAA+GGG | 0.563669 | 7:-62036178 | MsaT037635.1:CDS |
CGTCTTTATATACCTTTGAG+TGG | 0.567929 | 7:+62038934 | None:intergenic |
GGTGCGCCAGCTCTATCCCA+AGG | 0.568670 | 7:-62036179 | MsaT037635.1:CDS |
AGAAGATTTAATACCTGATG+AGG | 0.571874 | 7:+62035177 | None:intergenic |
TACTAAGCTTGGAAGACCAA+GGG | 0.579399 | 7:-62036201 | MsaT037635.1:CDS |
TAAAGTCTATAATAAGGTGG+TGG | 0.589868 | 7:+62038135 | None:intergenic |
TTGTAAAGTCTATAATAAGG+TGG | 0.624502 | 7:+62038132 | None:intergenic |
CTACTAAGCTTGGAAGACCA+AGG | 0.625337 | 7:-62036202 | MsaT037635.1:CDS |
TGCGCCAGCTCTATCCCAAG+GGG | 0.660989 | 7:-62036177 | MsaT037635.1:CDS |
ACTAAGCTTGGAAGACCAAG+GGG | 0.673301 | 7:-62036200 | MsaT037635.1:CDS |
TAGATTTCAAGAAGGAACTG+AGG | 0.694233 | 7:-62035338 | MsaT037635.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr7 | gene | 62034102 | 62038959 | 62034102 | ID=MsaG037635 |
Chr7 | mRNA | 62034102 | 62038959 | 62034102 | ID=MsaT037635.1;Parent=MsaG037635 |
Chr7 | exon | 62034102 | 62034162 | 62034102 | ID=MsaT037635.1.exon6;Parent=MsaT037635.1 |
Chr7 | CDS | 62034102 | 62034162 | 62034102 | ID=cds.MsaT037635.1;Parent=MsaT037635.1 |
Chr7 | exon | 62034998 | 62035071 | 62034998 | ID=MsaT037635.1.exon5;Parent=MsaT037635.1 |
Chr7 | CDS | 62034998 | 62035071 | 62034998 | ID=cds.MsaT037635.1;Parent=MsaT037635.1 |
Chr7 | exon | 62035191 | 62035357 | 62035191 | ID=MsaT037635.1.exon4;Parent=MsaT037635.1 |
Chr7 | CDS | 62035191 | 62035357 | 62035191 | ID=cds.MsaT037635.1;Parent=MsaT037635.1 |
Chr7 | exon | 62036167 | 62036236 | 62036167 | ID=MsaT037635.1.exon3;Parent=MsaT037635.1 |
Chr7 | CDS | 62036167 | 62036236 | 62036167 | ID=cds.MsaT037635.1;Parent=MsaT037635.1 |
Chr7 | exon | 62038047 | 62038171 | 62038047 | ID=MsaT037635.1.exon2;Parent=MsaT037635.1 |
Chr7 | CDS | 62038047 | 62038171 | 62038047 | ID=cds.MsaT037635.1;Parent=MsaT037635.1 |
Chr7 | exon | 62038947 | 62038959 | 62038947 | ID=MsaT037635.1.exon1;Parent=MsaT037635.1 |
Chr7 | CDS | 62038947 | 62038959 | 62038947 | ID=cds.MsaT037635.1;Parent=MsaT037635.1 |
Gene Sequence |
Protein sequence |