AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037638


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037638 MtrunA17_Chr6g0478701 73.826 149 13 1 1 123 1 149 5.02e-56 172
MsaG037638 MtrunA17_Chr2g0326811 73.154 149 14 1 1 123 1 149 2.22e-55 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037638 AT5G03500.3 68.456 149 21 1 1 123 1 149 1.22e-62 189
MsaG037638 AT5G03500.4 68.456 149 21 1 1 123 1 149 1.22e-62 189
MsaG037638 AT5G03500.2 68.456 149 21 1 1 123 1 149 1.22e-62 189
MsaG037638 AT5G03500.5 68.456 149 21 1 1 123 1 149 1.22e-62 189
MsaG037638 AT5G03500.7 68.456 149 21 1 1 123 1 149 1.22e-62 189
MsaG037638 AT5G03500.1 68.456 149 21 1 1 123 1 149 1.22e-62 189
MsaG037638 AT5G03500.6 68.456 149 21 1 1 123 11 159 1.24e-62 189
MsaG037638 AT5G03220.1 68.456 149 21 1 1 123 1 149 2.84e-55 171

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACATTGATTCATATTA+TGG 0.195694 7:-62064798 MsaT037638.1:CDS
TAGACTTTACAAATATTATT+TGG 0.223723 7:-62089449 MsaT037638.1:CDS
AGAACTGCAACTACACATTT+TGG 0.235567 7:-62065058 MsaT037638.1:CDS
GAAGGGACTTATATTTGTTT+TGG 0.300965 7:-62089388 MsaT037638.1:CDS
TCGATTGGCGGTGGAGGCTC+TGG 0.362896 7:+62089409 None:intergenic
TATTTGTAAAGTCTATAATA+AGG 0.399185 7:+62089457 None:intergenic
AAATTCATTGCATCCTCATC+AGG 0.403386 7:-62064944 MsaT037638.1:intron
CTATAATAAGGTGGTGGTGG+TGG 0.415430 7:+62089469 None:intergenic
TGTCTTCTACGGCTTGTTTG+CGG 0.449632 7:+62064759 None:intergenic
ATACCTCTTTATGTCTTCTA+CGG 0.455805 7:+62064748 None:intergenic
GTCCCTTCGATTGGCGGTGG+AGG 0.464706 7:+62089403 None:intergenic
GCAAGGACATTTCACTATGA+TGG 0.485863 7:+62064661 None:intergenic
GAGCCTCCACCGCCAATCGA+AGG 0.491154 7:-62089406 MsaT037638.1:CDS
TATAATAAGGTGGTGGTGGT+GGG 0.505040 7:+62089470 None:intergenic
CAAATATAAGTCCCTTCGAT+TGG 0.522975 7:+62089394 None:intergenic
AGTCTATAATAAGGTGGTGG+TGG 0.525802 7:+62089466 None:intergenic
ACTCTTCTTGCATACTGTGA+TGG 0.527495 7:+62065009 None:intergenic
AAGCCGTAGAAGACATAAAG+AGG 0.531060 7:-62064751 MsaT037638.1:intron
AGCCTCCACCGCCAATCGAA+GGG 0.535045 7:-62089405 MsaT037638.1:CDS
ATATAAGTCCCTTCGATTGG+CGG 0.542571 7:+62089397 None:intergenic
ATAAGAACAAAAGATTGCCT+AGG 0.554263 7:+62064685 None:intergenic
TAAGTCCCTTCGATTGGCGG+TGG 0.560515 7:+62089400 None:intergenic
TAAGAACAAAAGATTGCCTA+GGG 0.567732 7:+62064686 None:intergenic
AGAAGATTTAATACCTGATG+AGG 0.571874 7:+62064931 None:intergenic
CTTAATGGTACAGAAACCCT+AGG 0.581794 7:-62064702 MsaT037638.1:intron
TAAAGTCTATAATAAGGTGG+TGG 0.589868 7:+62089463 None:intergenic
TTGTAAAGTCTATAATAAGG+TGG 0.624502 7:+62089460 None:intergenic
TAGATTTCAAGAAGGAACTG+AGG 0.694233 7:-62065092 MsaT037638.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 62064675 62089509 62064675 ID=MsaG037638
Chr7 mRNA 62064675 62089509 62064675 ID=MsaT037638.1;Parent=MsaG037638
Chr7 exon 62064675 62064711 62064675 ID=MsaT037638.1.exon4;Parent=MsaT037638.1
Chr7 CDS 62064675 62064711 62064675 ID=cds.MsaT037638.1;Parent=MsaT037638.1
Chr7 exon 62064752 62064825 62064752 ID=MsaT037638.1.exon3;Parent=MsaT037638.1
Chr7 CDS 62064752 62064825 62064752 ID=cds.MsaT037638.1;Parent=MsaT037638.1
Chr7 exon 62064945 62065103 62064945 ID=MsaT037638.1.exon2;Parent=MsaT037638.1
Chr7 CDS 62064945 62065103 62064945 ID=cds.MsaT037638.1;Parent=MsaT037638.1
Chr7 exon 62089375 62089509 62089375 ID=MsaT037638.1.exon1;Parent=MsaT037638.1
Chr7 CDS 62089375 62089509 62089375 ID=cds.MsaT037638.1;Parent=MsaT037638.1
Gene Sequence

>MsaT037638.1

ATGGCAACAGCTACATACCCACCACCACCACCTTATTATAGACTTTACAAATATTATTTGGAAGATTCTGAATCTTCTCCAGAGCCTCCACCGCCAATCGAAGGGACTTATATTTGTTTTGGTGGAAATTATACGAAGGAACTGAGGTCACTTAATCGAGAACTGCAACTACACATTTTGGAGCTTGCTGATATTCTTATTGAGAGACCATCACAGTATGCAAGAAGAGTCGAAGAAGTTTCAACTTTATTCAAAAACTTGCATCATCTTTTAAATTCATTGCATCCTCATCAGGCAAGATCAACATTGATTCATATTATGGAACTTCAGATAGAGCGCCGCAAACAAGCCGTAGAAGACATAAAGAGAAACCCTAGGCAATCTTTTGTTCTTATGCCATCATAG

Protein sequence

>MsaT037638.1

MATATYPPPPPYYRLYKYYLEDSESSPEPPPPIEGTYICFGGNYTKELRSLNRELQLHILELADILIERPSQYARRVEEVSTLFKNLHHLLNSLHPHQARSTLIHIMELQIERRKQAVEDIKRNPRQSFVLMPS*