AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037703

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037703 MtrunA17_Chr1g0203051 37.945 253 144 3 50 301 18 258 1.45e-48 171
MsaG037703 MtrunA17_Chr1g0171741 47.917 144 74 1 108 251 119 261 1.11e-41 145
MsaG037703 MtrunA17_Chr8g0389811 43.411 129 73 0 99 227 61 189 6.70e-36 128
MsaG037703 MtrunA17_Chr2g0331371 44.361 133 74 0 94 226 27 159 3.40e-28 108
MsaG037703 MtrunA17_Chr2g0287651 30.726 179 124 0 113 291 66 244 3.00e-27 111
MsaG037703 MtrunA17_Chr6g0473421 41.221 131 68 2 74 197 2 130 5.18e-25 98.2
MsaG037703 MtrunA17_Chr1g0202341 29.279 222 142 5 94 304 15 232 2.79e-17 82.4
MsaG037703 MtrunA17_Chr1g0172931 32.653 147 82 3 60 204 27 158 1.70e-16 75.5
MsaG037703 MtrunA17_Chr1g0198761 30.952 168 101 6 134 289 59 223 3.76e-15 75.9
MsaG037703 MtrunA17_Chr5g0448761 28.743 167 117 2 126 290 64 230 5.50e-15 75.5
MsaG037703 MtrunA17_Chr5g0431001 30.303 165 102 5 133 286 72 234 6.16e-14 72.0
MsaG037703 MtrunA17_Chr2g0276841 31.138 167 103 6 134 292 82 244 8.89e-14 71.6
MsaG037703 MtrunA17_Chr2g0294841 32.414 145 86 5 127 262 50 191 5.96e-13 69.3
MsaG037703 MtrunA17_Chr8g0363841 30.769 156 102 3 134 287 29 180 2.53e-12 67.4
MsaG037703 MtrunA17_Chr8g0340801 39.130 69 42 0 233 301 1 69 3.44e-12 62.0
MsaG037703 MtrunA17_Chr6g0458391 26.923 208 130 8 50 248 20 214 9.42e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037703 AT4G38180.1 28.077 260 163 8 37 291 3 243 8.11e-15 75.5
MsaG037703 AT1G10240.2 29.167 168 107 6 133 292 59 222 1.02e-14 75.1
MsaG037703 AT1G10240.1 29.167 168 107 6 133 292 59 222 1.02e-14 75.1
MsaG037703 AT5G28530.3 27.273 165 108 5 134 291 67 226 2.01e-13 71.2
MsaG037703 AT5G28530.2 27.273 165 108 5 134 291 67 226 2.01e-13 71.2
MsaG037703 AT5G28530.1 27.273 165 108 5 134 291 67 226 2.23e-13 70.9
MsaG037703 AT5G28530.5 27.273 165 108 5 134 291 67 226 4.05e-13 70.1
MsaG037703 AT5G28530.4 27.273 165 108 5 134 291 67 226 4.10e-13 70.1

Find 49 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTTGAAACATCTTCAT+TGG 0.320552 7:-63476755 None:intergenic
GATGTTTCAAAGATGCAATT+TGG 0.353041 7:+63476763 MsaT037703.1:CDS
TGTTAAATCTGAAGCATTGC+GGG 0.366165 7:+63477073 MsaT037703.1:CDS
CCACATCTTGTTTCATGCTT+CGG 0.382644 7:-63476964 None:intergenic
GATGGAGAAGAGGGCTTTGT+TGG 0.391951 7:+63476529 MsaT037703.1:CDS
AGTGAAAGTGGGTTTGTTGA+TGG 0.398122 7:+63476574 MsaT037703.1:CDS
AAGATTCAAGTTAAGAACTT+TGG 0.409899 7:+63477132 MsaT037703.1:CDS
TTGAAACATCTTCATTGGTC+AGG 0.410269 7:-63476750 None:intergenic
GTTAAGAACTTTGGCAATGT+TGG 0.430183 7:+63477141 MsaT037703.1:CDS
TCTGAAGCATTGCGGGCTCT+TGG 0.447829 7:+63477080 MsaT037703.1:CDS
TGTTGGTTTGCAAAGATGAA+TGG 0.456596 7:+63476814 MsaT037703.1:CDS
TAAAGTAGGTTTCTTGAAGA+AGG 0.456624 7:+63477218 MsaT037703.1:CDS
AATGCAAATAACACCGATGA+AGG 0.470320 7:+63476616 MsaT037703.1:CDS
ATCAAAGTCACACAAATGAT+TGG 0.473157 7:+63477165 MsaT037703.1:CDS
GCAATTACGAAGGACTCATC+TGG 0.473205 7:-63476691 None:intergenic
GTTAATGATGTTGATGATGA+AGG 0.482514 7:+63476448 MsaT037703.1:CDS
GTTGGTGATGATGATGATGC+TGG 0.527437 7:+63476547 MsaT037703.1:CDS
ATTTCCTTCCAACCCTTCAT+CGG 0.534967 7:-63476629 None:intergenic
GAGGTTGGTGGTAATGATAT+TGG 0.543133 7:+63476418 MsaT037703.1:CDS
CTGACGCTAGAGGTGCTGTA+AGG 0.545157 7:+63477283 MsaT037703.1:CDS
GTGGTAATGATATTGGTGAA+GGG 0.551537 7:+63476425 MsaT037703.1:CDS
TGGTAATGATATTGGTGAAG+GGG 0.563989 7:+63476426 MsaT037703.1:CDS
TTGATACAAATTCTCAACGT+TGG 0.565950 7:+63477013 MsaT037703.1:CDS
AATGGATTTGTTGTACGCAA+AGG 0.568521 7:+63476832 MsaT037703.1:CDS
ACCACGCTTGCGTGCCTCTG+CGG 0.570833 7:-63476941 None:intergenic
TTAATGATGTTGATGATGAA+GGG 0.574070 7:+63476449 MsaT037703.1:CDS
AACACCGATGAAGGGTTGGA+AGG 0.575758 7:+63476625 MsaT037703.1:CDS
GGTGGTAATGATATTGGTGA+AGG 0.577650 7:+63476424 MsaT037703.1:CDS
GATGCTGGTAGTAGTGAAAG+TGG 0.586986 7:+63476562 MsaT037703.1:CDS
ACCGCAGAGGCACGCAAGCG+TGG 0.589040 7:+63476940 MsaT037703.1:CDS
GCATTTGCAAATGCTGCCGA+TGG 0.595646 7:+63477189 MsaT037703.1:CDS
GCGGGCTCTTGGCTGCACAC+AGG 0.603127 7:+63477091 MsaT037703.1:CDS
CATGAAACAAGATGTGGTTG+TGG 0.606083 7:+63476970 MsaT037703.1:CDS
ATGTTAAATCTGAAGCATTG+CGG 0.608516 7:+63477072 MsaT037703.1:CDS
GACATAAGAGACGAGGTTGG+TGG 0.609026 7:+63476406 MsaT037703.1:CDS
GCCGATGGGTATGATAAAGT+AGG 0.625170 7:+63477204 MsaT037703.1:CDS
AAATAACACCGATGAAGGGT+TGG 0.630492 7:+63476621 MsaT037703.1:CDS
ACCTACTTTATCATACCCAT+CGG 0.631628 7:-63477205 None:intergenic
ATGCTGGTAGTAGTGAAAGT+GGG 0.635645 7:+63476563 MsaT037703.1:CDS
ATGCAAATAACACCGATGAA+GGG 0.643045 7:+63476617 MsaT037703.1:CDS
CATTTGCAAATGCTGCCGAT+GGG 0.646524 7:+63477190 MsaT037703.1:CDS
GTCAATATCAGCAATTACGA+AGG 0.647225 7:-63476701 None:intergenic
AATGACATAAGAGACGAGGT+TGG 0.660867 7:+63476403 MsaT037703.1:CDS
GTGATGTCTTCTGACGCTAG+AGG 0.698711 7:+63477273 MsaT037703.1:CDS
AGAAATAGGGTTAACCGCAG+AGG 0.699737 7:+63476927 MsaT037703.1:CDS
CCGAAGCATGAAACAAGATG+TGG 0.716569 7:+63476964 MsaT037703.1:CDS
AATGAATGACATAAGAGACG+AGG 0.722234 7:+63476399 None:intergenic
TGATGCGAATGTTGATGACG+AGG 0.734293 7:+63476492 MsaT037703.1:CDS
TGCGAATGTTGATGACGAGG+AGG 0.737910 7:+63476495 MsaT037703.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 63476400 63477602 63476400 ID=MsaG037703
Chr7 mRNA 63476400 63477602 63476400 ID=MsaT037703.1;Parent=MsaG037703
Chr7 exon 63476400 63477304 63476400 ID=MsaT037703.1.exon1;Parent=MsaT037703.1
Chr7 CDS 63476400 63477304 63476400 ID=cds.MsaT037703.1;Parent=MsaT037703.1
Chr7 exon 63477590 63477602 63477590 ID=MsaT037703.1.exon2;Parent=MsaT037703.1
Chr7 CDS 63477590 63477602 63477590 ID=cds.MsaT037703.1;Parent=MsaT037703.1
Gene Sequence

>MsaT037703.1

ATGAATGACATAAGAGACGAGGTTGGTGGTAATGATATTGGTGAAGGGGTTAATGATGTTGATGATGAAGGGAGTTCTTCTGACGAAGATGCTGATGCGAATGTTGATGACGAGGAGGTAGATGATTTTGATGGAGAAGAGGGCTTTGTTGGTGATGATGATGATGCTGGTAGTAGTGAAAGTGGGTTTGTTGATGGTGATGAATGTTCTGATACAAATGCAAATAACACCGATGAAGGGTTGGAAGGAAATTCAAACAGTCTCAATGAATCAACATATTCAGATCAAAATCCAGATGAGTCCTTCGTAATTGCTGATATTGACGATATCGTAAAGATTGATCTGTTTAACCTGACCAATGAAGATGTTTCAAAGATGCAATTTGGTAGTCTAGTAGTTGCATACAAATTTTACTGTTGGTTTGCAAAGATGAATGGATTTGTTGTACGCAAAGGTCAAGTTATTAAAAACAAGAATGGAGAAGTTCTTCAACAAACATTTCTATGTAATTTTGAAGGTTTTAGGCAAGAAATAGGGTTAACCGCAGAGGCACGCAAGCGTGGTCCGAAGCATGAAACAAGATGTGGTTGTGGCGCAAAATTTCGAGTGCATATTGATACAAATTCTCAACGTTGGTACATCACAGTTTTCACTCTTGAGCACAATCATGATATGTTAAATCTGAAGCATTGCGGGCTCTTGGCTGCACACAGGAAGCTTACTAAGTCAGACAAGATTCAAGTTAAGAACTTTGGCAATGTTGGTATCAAAGTCACACAAATGATTGGTGCATTTGCAAATGCTGCCGATGGGTATGATAAAGTAGGTTTCTTGAAGAAGGATGTACATAATCAAATCTCAAGACAAAGAAAAGTGATGTCTTCTGACGCTAGAGGTGCTGTAAGTCAGTTTTTCTAA
Protein sequence

>MsaT037703.1

MNDIRDEVGGNDIGEGVNDVDDEGSSSDEDADANVDDEEVDDFDGEEGFVGDDDDAGSSESGFVDGDECSDTNANNTDEGLEGNSNSLNESTYSDQNPDESFVIADIDDIVKIDLFNLTNEDVSKMQFGSLVVAYKFYCWFAKMNGFVVRKGQVIKNKNGEVLQQTFLCNFEGFRQEIGLTAEARKRGPKHETRCGCGAKFRVHIDTNSQRWYITVFTLEHNHDMLNLKHCGLLAAHRKLTKSDKIQVKNFGNVGIKVTQMIGAFANAADGYDKVGFLKKDVHNQISRQRKVMSSDARGAVSQFF*