AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037757

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037757 MtrunA17_Chr7g0239061 95.147 680 33 0 1 680 1 680 0.0 1338
MsaG037757 MtrunA17_Chr4g0009751 70.111 629 160 8 13 637 18 622 0.0 837
MsaG037757 MtrunA17_Chr8g0346711 55.248 686 246 14 9 677 24 665 0.0 708
MsaG037757 MtrunA17_Chr2g0276781 67.956 362 110 4 9 367 18 376 1.06e-171 506
MsaG037757 MtrunA17_Chr2g0276781 42.021 188 95 6 485 664 461 642 1.58e-33 137
MsaG037757 MtrunA17_Chr8g0385791 61.702 376 134 7 8 378 53 423 3.72e-151 459
MsaG037757 MtrunA17_Chr8g0385791 38.725 204 104 6 477 661 601 802 5.46e-32 132
MsaG037757 MtrunA17_Chr2g0285461 49.733 374 178 8 6 373 15 384 7.12e-108 347
MsaG037757 MtrunA17_Chr2g0285461 35.000 120 70 4 560 672 765 883 1.36e-14 77.8
MsaG037757 MtrunA17_Chr8g0376771 49.072 377 175 8 6 373 15 383 5.76e-106 343
MsaG037757 MtrunA17_Chr8g0376771 38.710 93 54 2 569 659 780 871 1.31e-14 77.8
MsaG037757 MtrunA17_Chr5g0433301 50.287 348 165 6 5 346 15 360 3.44e-105 339
MsaG037757 MtrunA17_Chr5g0433301 40.506 79 45 1 581 657 725 803 9.71e-14 75.1
MsaG037757 MtrunA17_Chr3g0109391 45.433 416 210 10 11 418 23 429 7.82e-105 338
MsaG037757 MtrunA17_Chr3g0109391 38.298 94 55 2 566 657 719 811 1.88e-13 73.9
MsaG037757 MtrunA17_Chr4g0046371 43.552 411 193 9 14 397 38 436 7.01e-104 332
MsaG037757 MtrunA17_Chr4g0029671 45.257 369 183 7 12 365 52 416 6.76e-103 332
MsaG037757 MtrunA17_Chr4g0029671 42.857 140 74 3 527 660 615 754 4.48e-26 114
MsaG037757 MtrunA17_Chr2g0326281 45.013 371 175 7 12 355 54 422 1.39e-100 326
MsaG037757 MtrunA17_Chr2g0326281 53.261 92 42 1 570 660 695 786 8.53e-25 110
MsaG037757 MtrunA17_Chr1g0156321 46.705 349 179 5 1 344 30 376 5.93e-95 312
MsaG037757 MtrunA17_Chr1g0156321 43.299 97 51 3 570 663 754 849 2.98e-15 79.7
MsaG037757 MtrunA17_Chr2g0282961 45.082 366 178 7 14 366 53 408 4.17e-94 303
MsaG037757 MtrunA17_Chr4g0070181 46.779 357 180 7 6 357 15 366 1.33e-88 300
MsaG037757 MtrunA17_Chr4g0070181 46.154 91 46 2 570 658 994 1083 9.42e-18 88.2
MsaG037757 MtrunA17_Chr2g0301251 46.839 348 175 7 6 347 15 358 1.76e-88 299
MsaG037757 MtrunA17_Chr2g0301251 43.269 104 52 3 570 667 986 1088 8.11e-18 88.2
MsaG037757 MtrunA17_Chr8g0389081 46.334 341 175 6 14 348 32 370 2.56e-84 287
MsaG037757 MtrunA17_Chr8g0389081 48.352 91 44 2 570 658 985 1074 9.42e-19 91.3
MsaG037757 MtrunA17_Chr2g0326761 37.346 407 206 9 8 373 5 403 1.69e-70 243
MsaG037757 MtrunA17_Chr4g0028971 35.845 438 209 13 3 379 1 427 4.52e-67 233
MsaG037757 MtrunA17_Chr7g0264171 34.541 414 206 12 14 371 31 435 2.88e-63 221
MsaG037757 MtrunA17_Chr1g0199681 35.922 412 207 12 3 369 83 482 1.51e-60 215
MsaG037757 MtrunA17_Chr3g0115271 34.459 444 241 14 9 414 14 445 1.77e-60 213
MsaG037757 MtrunA17_Chr1g0181211 34.018 438 219 14 8 384 21 449 8.03e-58 207
MsaG037757 MtrunA17_Chr5g0417281 34.770 348 201 8 7 346 4 333 3.86e-57 202
MsaG037757 MtrunA17_Chr7g0264161 38.111 307 177 7 24 324 2 301 7.18e-56 192
MsaG037757 MtrunA17_Chr5g0424931 30.635 457 255 14 14 434 19 449 1.56e-55 198
MsaG037757 MtrunA17_Chr5g0436761 32.432 370 226 7 3 363 1 355 2.46e-53 190
MsaG037757 MtrunA17_Chr8g0356211 41.667 228 121 6 124 346 2 222 8.29e-52 182
MsaG037757 MtrunA17_Chr1g0178011 31.564 358 228 9 14 366 12 357 7.40e-48 175
MsaG037757 MtrunA17_Chr2g0289221 33.742 326 177 12 14 324 20 321 3.28e-44 162
MsaG037757 MtrunA17_Chr5g0417211 33.434 332 173 11 14 325 12 315 5.77e-43 160
MsaG037757 MtrunA17_Chr5g0424801 29.722 360 229 11 14 366 12 354 5.45e-39 148
MsaG037757 MtrunA17_Chr2g0277461 28.382 377 244 9 14 380 13 373 5.13e-38 147
MsaG037757 MtrunA17_Chr5g0425261 29.722 360 229 11 14 366 12 354 9.47e-38 144
MsaG037757 MtrunA17_Chr5g0424741 27.344 384 229 12 14 357 12 385 8.87e-36 142
MsaG037757 MtrunA17_Chr5g0425661 34.951 206 126 3 14 216 37 237 2.06e-34 131
MsaG037757 MtrunA17_Chr2g0277531 28.571 315 200 10 9 314 15 313 1.81e-32 128
MsaG037757 MtrunA17_Chr8g0340731 31.250 112 55 1 573 662 158 269 7.11e-13 69.7
MsaG037757 MtrunA17_Chr4g0009741 82.500 40 5 2 642 679 1 40 8.98e-12 60.1
MsaG037757 MtrunA17_Chr5g0424791 29.452 146 95 2 67 212 14 151 1.70e-11 62.8
MsaG037757 MtrunA17_Chr1g0193861 35.955 89 48 2 568 648 81 168 2.39e-11 63.2
MsaG037757 MtrunA17_Chr1g0156301 38.947 95 42 3 570 663 177 256 3.67e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037757 AT4G23980.2 56.716 670 226 16 2 663 1 614 0.0 681
MsaG037757 AT4G23980.1 56.567 670 229 16 2 663 1 616 0.0 681
MsaG037757 AT1G59750.2 48.974 682 260 17 11 667 19 637 0.0 597
MsaG037757 AT1G59750.1 48.986 690 251 18 11 667 19 640 0.0 596
MsaG037757 AT1G59750.3 48.986 690 251 18 11 667 19 640 0.0 596
MsaG037757 AT1G35540.1 44.544 669 278 15 5 663 18 603 0.0 546
MsaG037757 AT1G34410.1 44.765 659 274 12 5 654 18 595 0.0 545
MsaG037757 AT1G34310.1 45.008 651 272 13 5 648 18 589 0.0 534
MsaG037757 AT1G35520.1 44.977 647 266 12 11 648 24 589 0.0 533
MsaG037757 AT3G61830.1 70.083 361 103 3 8 365 19 377 0.0 530
MsaG037757 AT3G61830.1 50.847 177 54 6 493 667 439 584 1.45e-39 154
MsaG037757 AT2G46530.1 70.492 366 102 4 8 370 15 377 0.0 529
MsaG037757 AT2G46530.1 44.041 193 85 5 473 663 412 583 1.47e-36 145
MsaG037757 AT2G46530.3 70.492 366 102 4 8 370 36 398 0.0 529
MsaG037757 AT2G46530.3 44.041 193 85 5 473 663 433 604 1.77e-36 145
MsaG037757 AT1G35240.1 44.190 654 271 14 5 654 18 581 1.24e-180 526
MsaG037757 AT1G34390.1 45.161 651 269 13 5 648 18 587 3.74e-179 523
MsaG037757 AT1G59750.4 64.820 361 121 4 11 367 19 377 1.36e-163 485
MsaG037757 AT1G59750.4 42.564 195 90 7 485 667 451 635 2.27e-34 139
MsaG037757 AT3G61830.2 69.817 328 94 3 41 365 1 326 2.68e-160 473
MsaG037757 AT3G61830.2 50.847 177 54 6 493 667 388 533 6.83e-40 154
MsaG037757 AT5G62000.2 63.085 363 125 6 8 365 55 413 7.61e-158 477
MsaG037757 AT5G62000.2 38.961 231 119 8 462 673 609 836 4.28e-37 149
MsaG037757 AT5G62000.5 63.085 363 125 6 8 365 55 413 7.61e-158 477
MsaG037757 AT5G62000.5 38.961 231 119 8 462 673 609 836 4.28e-37 149
MsaG037757 AT5G62000.3 63.085 363 125 6 8 365 55 413 7.61e-158 477
MsaG037757 AT5G62000.3 38.961 231 119 8 462 673 609 836 4.28e-37 149
MsaG037757 AT5G62000.1 63.085 363 125 6 8 365 55 413 7.61e-158 477
MsaG037757 AT5G62000.1 38.961 231 119 8 462 673 609 836 4.28e-37 149
MsaG037757 AT5G62000.4 63.085 363 125 6 8 365 55 413 2.10e-157 476
MsaG037757 AT5G62000.4 39.130 230 118 8 462 672 609 835 1.36e-36 147
MsaG037757 AT2G46530.2 68.512 289 84 4 86 370 5 290 1.89e-131 397
MsaG037757 AT2G46530.2 44.041 193 85 5 473 663 325 496 3.66e-37 146
MsaG037757 AT1G34170.1 41.538 520 225 14 2 501 1 461 4.91e-124 378
MsaG037757 AT1G34170.2 39.423 520 210 15 2 501 1 435 6.40e-109 338
MsaG037757 AT1G34170.3 39.423 520 210 15 2 501 1 435 7.32e-108 338
MsaG037757 AT1G34170.3 64.835 91 32 0 573 663 436 526 7.02e-34 137
MsaG037757 AT1G30330.1 45.227 440 207 12 6 429 15 436 4.59e-106 343
MsaG037757 AT1G30330.1 39.583 96 55 2 564 657 788 882 1.08e-14 78.6
MsaG037757 AT1G30330.3 45.227 440 207 12 6 429 17 438 5.76e-106 343
MsaG037757 AT1G30330.3 39.583 96 55 2 564 657 790 884 1.14e-14 78.6
MsaG037757 AT1G30330.2 45.227 440 207 12 6 429 17 438 5.76e-106 343
MsaG037757 AT1G30330.2 39.583 96 55 2 564 657 790 884 1.14e-14 78.6
MsaG037757 AT5G37020.2 47.558 389 190 10 6 384 12 396 6.51e-106 339
MsaG037757 AT5G37020.2 32.639 144 84 6 496 636 639 772 4.56e-11 66.6
MsaG037757 AT5G37020.1 47.558 389 190 10 6 384 12 396 2.40e-105 339
MsaG037757 AT5G37020.1 34.545 165 95 6 496 657 639 793 2.76e-16 83.6
MsaG037757 AT5G60450.2 45.623 377 184 10 3 364 30 400 3.12e-99 322
MsaG037757 AT5G60450.2 43.333 120 64 2 555 673 626 742 7.30e-25 110
MsaG037757 AT5G60450.1 46.341 369 177 10 11 364 62 424 6.75e-98 318
MsaG037757 AT5G60450.1 41.270 126 70 2 555 679 650 772 8.83e-25 110
MsaG037757 AT1G19850.2 47.941 340 163 7 14 344 43 377 1.18e-94 312
MsaG037757 AT1G19850.2 44.330 97 50 4 570 663 782 877 1.51e-15 81.3
MsaG037757 AT1G19850.1 47.941 340 163 7 14 344 54 388 1.41e-94 312
MsaG037757 AT1G19850.1 44.330 97 50 4 570 663 793 888 1.56e-15 81.3
MsaG037757 AT2G33860.1 45.217 345 167 7 14 345 54 389 5.09e-93 301
MsaG037757 AT5G20730.2 45.902 366 186 8 6 366 16 374 1.87e-88 300
MsaG037757 AT5G20730.2 46.154 91 46 3 570 658 1037 1126 4.64e-17 86.3
MsaG037757 AT5G20730.3 45.777 367 186 8 6 366 16 375 3.68e-88 299
MsaG037757 AT5G20730.3 46.154 91 46 3 570 658 1038 1127 4.78e-17 86.3
MsaG037757 AT5G20730.1 45.777 367 186 8 6 366 16 375 4.51e-88 299
MsaG037757 AT5G20730.1 46.154 91 46 3 570 658 1038 1127 4.77e-17 86.3
MsaG037757 AT1G19220.1 45.600 375 190 9 4 369 13 382 7.91e-88 297
MsaG037757 AT1G19220.1 46.078 102 48 3 559 658 951 1047 5.70e-19 92.4
MsaG037757 AT5G37020.3 48.763 283 135 7 108 384 23 301 8.45e-75 255
MsaG037757 AT5G37020.3 28.125 256 142 8 434 657 453 698 1.88e-16 84.0
MsaG037757 AT1G43950.1 53.302 212 91 4 5 215 18 222 3.10e-70 228
MsaG037757 AT2G33860.2 48.498 233 115 3 116 345 2 232 8.66e-69 232
MsaG037757 AT4G30080.1 35.766 411 209 11 3 367 8 409 4.16e-66 230
MsaG037757 AT2G28350.2 37.073 410 200 10 7 367 3 403 7.77e-66 229
MsaG037757 AT2G28350.1 37.073 410 200 10 7 367 3 403 9.53e-66 230
MsaG037757 AT1G77850.1 30.896 424 235 12 10 391 15 422 5.50e-52 189
MsaG037757 AT1G77850.2 30.896 424 235 12 10 391 15 422 6.02e-52 190
MsaG037757 AT5G25890.1 34.884 86 56 0 573 658 83 168 2.54e-13 69.3
MsaG037757 AT3G17600.1 41.026 78 44 1 573 648 75 152 3.36e-11 62.4
MsaG037757 AT3G16500.1 34.000 100 48 2 573 654 154 253 5.89e-11 64.3
MsaG037757 AT3G16500.2 32.143 112 58 2 561 654 142 253 6.01e-11 63.9

Find 150 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAGTGACGTTCCAGATTT+TGG 0.157571 7:+64850865 MsaT037757.1:CDS
TTGAGATATCAAACTTATTT+TGG 0.174168 7:-64851585 None:intergenic
GACTCTACTTACTGGGAAAA+TGG 0.200322 7:+64851112 MsaT037757.1:CDS
AGATCCATCCTAGGAATAAA+TGG 0.234682 7:+64851982 MsaT037757.1:CDS
TGTTGTCAATGTTCATTTAC+TGG 0.249472 7:+64848352 MsaT037757.1:CDS
CACACTGGTTAAATTCAAAA+TGG 0.268060 7:+64850600 MsaT037757.1:CDS
CTCCTGCTGCAAAATATAAA+AGG 0.298744 7:+64850833 MsaT037757.1:CDS
TCCGCTTCCTTACCAAAATC+TGG 0.302105 7:-64850877 None:intergenic
TTGCTGAAGGAGTGAGTTCT+TGG 0.304040 7:-64849047 None:intergenic
CACTTTAAGCATATATTTAG+AGG 0.306214 7:+64849314 MsaT037757.1:CDS
ATGCCTTCATCTGTGATTTC+CGG 0.325895 7:+64849953 MsaT037757.1:CDS
TATAAGTGTGAATAAGTATA+TGG 0.342092 7:+64850167 MsaT037757.1:CDS
CAGTAGGTGACTCTACTTAC+TGG 0.347101 7:+64851104 MsaT037757.1:CDS
TAGTGATGAGATAGGTAATT+TGG 0.349545 7:-64852349 None:intergenic
TACATCCTTACATTAGATTC+TGG 0.353577 7:-64848883 None:intergenic
TGCTTGCTTTGTGTTTCATT+TGG 0.354289 7:-64851649 None:intergenic
AAGGAGTGAGTTCTTGGTCT+TGG 0.354402 7:-64849041 None:intergenic
CTTAGTGCAACTTCTGCTAT+TGG 0.357778 7:-64851674 None:intergenic
ATTCCTGAGTTGGAGTAGAT+TGG 0.370432 7:-64849259 None:intergenic
CGTCGACACTTGCTCACAAC+TGG 0.370955 7:+64849680 MsaT037757.1:CDS
GAGTATGCAGCAAATGTATT+TGG 0.374703 7:+64851511 MsaT037757.1:CDS
ACAATTTCCCATTTATTCCT+AGG 0.375607 7:-64851990 None:intergenic
CTACAAGCTGTAGATTGTTC+GGG 0.378856 7:+64851446 MsaT037757.1:CDS
ATCTACTCCAACTCAGGAAT+TGG 0.380361 7:+64849262 MsaT037757.1:CDS
TATATTCTCATCAGGTTCAA+TGG 0.382829 7:+64850722 MsaT037757.1:intron
TATTCACACTTATAATGAAC+TGG 0.400736 7:-64850158 None:intergenic
GCTACAAGCTGTAGATTGTT+CGG 0.405817 7:+64851445 MsaT037757.1:CDS
TCCCATGGTGAAATTCTATC+AGG 0.415551 7:-64850769 None:intergenic
GTTAGAAGTATCAACAAATC+AGG 0.423287 7:+64848277 MsaT037757.1:intron
GGACAAGGATCAAGGCTTGT+AGG 0.425783 7:-64849008 None:intergenic
ATGAACTCTATGAACAGTTA+TGG 0.431151 7:+64848001 MsaT037757.1:CDS
CTTGGTCTTGGAGGTTCGGC+AGG 0.433704 7:-64849029 None:intergenic
AGTTCTTGGTCTTGGAGGTT+CGG 0.435328 7:-64849033 None:intergenic
GAGTGAGTTCTTGGTCTTGG+AGG 0.436921 7:-64849038 None:intergenic
GTTTCTTCTAGTGATGAGAT+AGG 0.438418 7:-64852357 None:intergenic
TTCCTGAGTTGGAGTAGATT+GGG 0.444751 7:-64849258 None:intergenic
GATGAAGGCATGCAGCTTTG+TGG 0.446909 7:-64849941 None:intergenic
TGTGTTTCATTTGGAGATAT+CGG 0.452653 7:-64851640 None:intergenic
GATTATGTTATTACCAGTGA+TGG 0.454300 7:-64849143 None:intergenic
TGGTCCTTCTCATTTGTGTC+AGG 0.459330 7:+64851297 MsaT037757.1:CDS
GGAATGACGGTATGCATTTG+TGG 0.463307 7:+64851188 MsaT037757.1:CDS
AGCACAATGATGATGAATAA+TGG 0.463577 7:+64847970 None:intergenic
GGTGAAATTCTATCAGGTCT+TGG 0.465108 7:-64850763 None:intergenic
CGTAGGAGCAGAAGCCAGAA+GGG 0.466622 7:-64850800 None:intergenic
AGAGAAGCTGTTTGACATGA+AGG 0.473975 7:+64851956 MsaT037757.1:CDS
CCAAAATCTGGAACGTCACT+AGG 0.476389 7:-64850865 None:intergenic
AACTCAGGAATTGGTTGCTA+AGG 0.479871 7:+64849271 MsaT037757.1:CDS
ATATTCTCATCAGGTTCAAT+GGG 0.480064 7:+64850723 MsaT037757.1:intron
ACTCTACTTACTGGGAAAAT+GGG 0.480110 7:+64851113 MsaT037757.1:CDS
CACAGAATGCCTTCCATCAC+TGG 0.481144 7:+64849130 MsaT037757.1:CDS
TCAAATGCAGGGTGTTGCAG+TGG 0.481218 7:+64851878 MsaT037757.1:CDS
AACACGACAAAGGATCTTGT+TGG 0.481262 7:-64848333 None:intergenic
ATGGGAGATAGAACCCCTTC+TGG 0.482103 7:+64850786 MsaT037757.1:CDS
TTTCCCATTTATTCCTAGGA+TGG 0.483618 7:-64851986 None:intergenic
CAATGATGATTTGACTGATC+AGG 0.484605 7:+64851396 MsaT037757.1:CDS
GCTGCATACTCAGACACCTT+AGG 0.488235 7:-64851499 None:intergenic
CATGTTGAAGTTCCTCGTGC+TGG 0.488356 7:+64848042 MsaT037757.1:CDS
TTAGCAACCAATTCCTGAGT+TGG 0.491444 7:-64849269 None:intergenic
CTGTCAAAAGGTTCAAATGC+AGG 0.492047 7:+64851866 MsaT037757.1:intron
GATCCATCCTAGGAATAAAT+GGG 0.495100 7:+64851983 MsaT037757.1:CDS
ATATTATGTCTGAAAACATA+AGG 0.495436 7:+64851167 MsaT037757.1:CDS
GATGTGCACAAAATGAGCTC+AGG 0.496139 7:+64852324 MsaT037757.1:CDS
GGAATCTGACTGACGGAAGT+TGG 0.500296 7:+64851254 MsaT037757.1:CDS
TCCAAGGTGCATGCTCTGAC+CGG 0.503939 7:-64849972 None:intergenic
ACACGACAAAGGATCTTGTT+GGG 0.504393 7:-64848332 None:intergenic
CTGCAAAATATAAAAGGCCT+AGG 0.510234 7:+64850839 MsaT037757.1:CDS
GTCCCAATCTACTCCAACTC+AGG 0.512152 7:+64849256 MsaT037757.1:CDS
GTCTGCATCAGTTCTTGACA+TGG 0.513254 7:-64851563 None:intergenic
TCTCAAAGGCTTCAAAAGAA+AGG 0.513575 7:+64851602 MsaT037757.1:CDS
CAGCATGAGACGCGGTTGCA+AGG 0.515240 7:-64849997 None:intergenic
TAGCAAGAGTGTCGCAGTCT+CGG 0.516524 7:+64851333 MsaT037757.1:CDS
TGTTGCAGTGGGTCGTGCGG+TGG 0.520688 7:+64851890 MsaT037757.1:CDS
AACTCTGATACAAGTACTCA+TGG 0.524293 7:+64849080 MsaT037757.1:CDS
GTTCGGCAGGACAAGGATCA+AGG 0.528735 7:-64849016 None:intergenic
ATTGTCTTCACTGATGATGA+AGG 0.528983 7:+64852008 MsaT037757.1:CDS
TTGTAGTATACTACAAGCCC+AGG 0.533426 7:+64850038 MsaT037757.1:CDS
CTTGGAGGTTCGGCAGGACA+AGG 0.533568 7:-64849023 None:intergenic
TAAAGAGATACTCACGGCCA+TGG 0.533691 7:-64852056 None:intergenic
AGGATCTTCACAATTATGAA+TGG 0.534136 7:+64849291 MsaT037757.1:CDS
ATTATTAACTTGTGTTACAT+CGG 0.539558 7:-64851146 None:intergenic
GAAGGGGATATGATGCTTGT+TGG 0.542834 7:+64852026 MsaT037757.1:CDS
TGACCGGAAATCACAGATGA+AGG 0.543721 7:-64849956 None:intergenic
GAGAAGCTGTTTGACATGAA+GGG 0.548993 7:+64851957 MsaT037757.1:CDS
AGAGTGTCGCAGTCTCGGCT+TGG 0.549847 7:+64851338 MsaT037757.1:CDS
TCGTAGGAGCAGAAGCCAGA+AGG 0.562413 7:-64850801 None:intergenic
CTATCTTCTCCTCATTCAAG+TGG 0.562928 7:+64851277 MsaT037757.1:CDS
GACACTTGCTCACAACTGGA+TGG 0.564774 7:+64849684 MsaT037757.1:CDS
GAGAATTACGCGTTGGCGTG+AGG 0.565139 7:+64849906 MsaT037757.1:CDS
CAATAGCAGAAGTTGCACTA+AGG 0.566869 7:+64851675 MsaT037757.1:CDS
TGGCACAATAATTGGACAAG+AGG 0.567142 7:+64850568 MsaT037757.1:CDS
TTGTCTTCACTGATGATGAA+GGG 0.569768 7:+64852009 MsaT037757.1:CDS
TTCAAAATGGAGATCACTCA+AGG 0.575917 7:+64850613 MsaT037757.1:CDS
GTTAGTTAAGACCTTGCTGA+AGG 0.576070 7:-64849060 None:intergenic
TGAAGGGACAGATCCATCCT+AGG 0.576311 7:+64851973 MsaT037757.1:CDS
ACGCGGTTGCAAGGACTCCA+AGG 0.576436 7:-64849988 None:intergenic
ACTGATAATAGCAGCAGCAA+TGG 0.577521 7:+64851223 MsaT037757.1:CDS
GGAACGTCACTAGGTAGCCT+AGG 0.578373 7:-64850856 None:intergenic
GGTTGTCAAGGAGAACAAAA+CGG 0.580030 7:+64851417 MsaT037757.1:CDS
TGAGAAGGACCACTTGAATG+AGG 0.580259 7:-64851286 None:intergenic
AGTAGGTGACTCTACTTACT+GGG 0.580726 7:+64851105 MsaT037757.1:CDS
ATTCTTCTGAGACTGACAAA+AGG 0.581364 7:+64850250 MsaT037757.1:CDS
TTTGACTGATCAGGTTGTCA+AGG 0.582263 7:+64851405 MsaT037757.1:CDS
CACAATGGAGAATTACGCGT+TGG 0.583091 7:+64849899 MsaT037757.1:CDS
TCCTCGCACGAACACTCCTA+AGG 0.585464 7:+64851483 MsaT037757.1:CDS
TGCGGTGGACTTGACTGCGT+TGG 0.588732 7:+64851905 MsaT037757.1:CDS
GAGATACGTTTGTGTTTCTG+AGG 0.590708 7:+64849741 MsaT037757.1:CDS
CAAATGCAGGGTGTTGCAGT+GGG 0.591983 7:+64851879 MsaT037757.1:CDS
TTGTTGGGAAGTTTGAACAA+TGG 0.592548 7:-64848317 None:intergenic
ATGTTATTACCAGTGATGGA+AGG 0.593428 7:-64849139 None:intergenic
GTATTTGGAGTCACCTCTGA+GGG 0.593824 7:+64851526 MsaT037757.1:CDS
GTGGACTTGACTGCGTTGGA+TGG 0.595313 7:+64851909 MsaT037757.1:CDS
ATCAGTTCTTGACATGGGAG+CGG 0.595334 7:-64851557 None:intergenic
GCAGGAGGTTGAATAGTCGT+AGG 0.595409 7:-64850817 None:intergenic
TGTCAAAAGGTTCAAATGCA+GGG 0.595922 7:+64851867 MsaT037757.1:intron
TCTGCATCAGTTCTTGACAT+GGG 0.598480 7:-64851562 None:intergenic
AAATAAGTTTGATATCTCAA+AGG 0.603379 7:+64851588 MsaT037757.1:CDS
TGCTTGTTGGTGATGATCCA+TGG 0.605617 7:+64852039 MsaT037757.1:CDS
AAGAGGATATTTCTTCACAC+TGG 0.605848 7:+64850585 MsaT037757.1:CDS
ATGTGCACAAAATGAGCTCA+GGG 0.609204 7:+64852325 MsaT037757.1:CDS
TGTCTTCACTGATGATGAAG+GGG 0.610189 7:+64852010 MsaT037757.1:CDS
ACCTGATAGAATTTCACCAT+GGG 0.610284 7:+64850768 MsaT037757.1:CDS
TCTGAAAACATAAGGAATGA+CGG 0.610544 7:+64851175 MsaT037757.1:CDS
ACTTGTTCCATATGTCCTTG+AGG 0.614119 7:-64848084 None:intergenic
TGCGACACTCTTGCTATCGT+CGG 0.614271 7:-64851326 None:intergenic
AGAAGGGGTTCTATCTCCCA+TGG 0.616888 7:-64850784 None:intergenic
TCCGGTCAGAGCATGCACCT+TGG 0.619758 7:+64849971 MsaT037757.1:CDS
GGGTGTTGCAGTGGGTCGTG+CGG 0.626070 7:+64851887 MsaT037757.1:CDS
ACCTTAGGAGTGTTCGTGCG+AGG 0.626867 7:-64851484 None:intergenic
CAGTTATGGAAAGCTTGTGC+TGG 0.627540 7:+64848015 MsaT037757.1:CDS
GAACATTGACAACACGACAA+AGG 0.628833 7:-64848343 None:intergenic
CAAATCAGGAACTAAATCAG+AGG 0.630877 7:+64848291 MsaT037757.1:CDS
CAGTGATGGAAGGCATTCTG+TGG 0.636814 7:-64849129 None:intergenic
GGTTTATGCACAGATAACTC+TGG 0.642878 7:+64848858 MsaT037757.1:CDS
GTGTCCTGACACAAATGAGA+AGG 0.645568 7:-64851301 None:intergenic
GCTGTTTATAGAGGGCACAA+TGG 0.646019 7:+64849884 MsaT037757.1:intron
AGCGGCAACGCATCCCTCAG+AGG 0.650317 7:-64851539 None:intergenic
TTATCGAGGAATCTGACTGA+CGG 0.657132 7:+64851247 MsaT037757.1:CDS
TGTATTTGGAGTCACCTCTG+AGG 0.658303 7:+64851525 MsaT037757.1:CDS
GCACGAGGAACTTCAACATG+AGG 0.659171 7:-64848039 None:intergenic
GACCTGATAGAATTTCACCA+TGG 0.666067 7:+64850767 MsaT037757.1:CDS
GGCTGAACAAGAAACAGATG+AGG 0.666478 7:+64848837 MsaT037757.1:intron
AGTTGCAACAGCATGAGACG+CGG 0.666713 7:-64850005 None:intergenic
TTCAAAGGAGACACAACAGT+AGG 0.668217 7:+64851088 MsaT037757.1:intron
AAGAACTCACTCCTTCAGCA+AGG 0.678844 7:+64849049 MsaT037757.1:CDS
GTAGGAGCAGAAGCCAGAAG+GGG 0.683231 7:-64850799 None:intergenic
GCAGCAGCAATGGTTTATCG+AGG 0.685062 7:+64851233 MsaT037757.1:CDS
AAAACTCTTTGTCCAGCACG+AGG 0.695811 7:-64848054 None:intergenic
TCTGATACAAGTACTCATGG+TGG 0.729409 7:+64849083 MsaT037757.1:CDS
GTTGTGAGCAAGTGTCGACG+TGG 0.742837 7:-64849677 None:intergenic
TGTCAAGGAGAACAAAACGG+AGG 0.766105 7:+64851420 MsaT037757.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 64847976 64852410 64847976 ID=MsaG037757
Chr7 mRNA 64847976 64852410 64847976 ID=MsaT037757.1;Parent=MsaG037757
Chr7 exon 64847976 64848104 64847976 ID=MsaT037757.1.exon1;Parent=MsaT037757.1
Chr7 CDS 64847976 64848104 64847976 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64848278 64848373 64848278 ID=MsaT037757.1.exon2;Parent=MsaT037757.1
Chr7 CDS 64848278 64848373 64848278 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64848838 64848894 64848838 ID=MsaT037757.1.exon3;Parent=MsaT037757.1
Chr7 CDS 64848838 64848894 64848838 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64848999 64849151 64848999 ID=MsaT037757.1.exon4;Parent=MsaT037757.1
Chr7 CDS 64848999 64849151 64848999 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64849251 64849335 64849251 ID=MsaT037757.1.exon5;Parent=MsaT037757.1
Chr7 CDS 64849251 64849335 64849251 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64849672 64849762 64849672 ID=MsaT037757.1.exon6;Parent=MsaT037757.1
Chr7 CDS 64849672 64849762 64849672 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64849895 64850059 64849895 ID=MsaT037757.1.exon7;Parent=MsaT037757.1
Chr7 CDS 64849895 64850059 64849895 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64850152 64850271 64850152 ID=MsaT037757.1.exon8;Parent=MsaT037757.1
Chr7 CDS 64850152 64850271 64850152 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64850562 64850634 64850562 ID=MsaT037757.1.exon9;Parent=MsaT037757.1
Chr7 CDS 64850562 64850634 64850562 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64850736 64850886 64850736 ID=MsaT037757.1.exon10;Parent=MsaT037757.1
Chr7 CDS 64850736 64850886 64850736 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64851095 64851696 64851095 ID=MsaT037757.1.exon11;Parent=MsaT037757.1
Chr7 CDS 64851095 64851696 64851095 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64851876 64852063 64851876 ID=MsaT037757.1.exon12;Parent=MsaT037757.1
Chr7 CDS 64851876 64852063 64851876 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Chr7 exon 64852278 64852410 64852278 ID=MsaT037757.1.exon13;Parent=MsaT037757.1
Chr7 CDS 64852278 64852410 64852278 ID=cds.MsaT037757.1;Parent=MsaT037757.1
Gene Sequence

>MsaT037757.1

ATGATGATGAATAATGGTGAAGAAAATGAACTCTATGAACAGTTATGGAAAGCTTGTGCTGGTCCTCATGTTGAAGTTCCTCGTGCTGGACAAAGAGTTTTCTATTTTCCTCAAGGACATATGGAACAATTAGAAGTATCAACAAATCAGGAACTAAATCAGAGGATTCCATTGTTCAAACTTCCCAACAAGATCCTTTGTCGTGTTGTCAATGTTCATTTACTGGCTGAACAAGAAACAGATGAGGTTTATGCACAGATAACTCTGGTACCAGAATCTAATCAAACTGAGCCTACAAGCCTTGATCCTTGTCCTGCCGAACCTCCAAGACCAAGAACTCACTCCTTCAGCAAGGTCTTAACTAACTCTGATACAAGTACTCATGGTGGCTTTTCTGTTCTTCGGAAACATGCCACAGAATGCCTTCCATCACTGGATATGTCCCAATCTACTCCAACTCAGGAATTGGTTGCTAAGGATCTTCACAATTATGAATGGCACTTTAAGCATATATTTAGAGGTCAACCACGTCGACACTTGCTCACAACTGGATGGAGTACGTTCGTGACTTCAAAAAGATTAGTTGCTGGAGATACGTTTGTGTTTCTGAGAGGGCACAATGGAGAATTACGCGTTGGCGTGAGGCATCTTGCTTCTCCACAAAGCTGCATGCCTTCATCTGTGATTTCCGGTCAGAGCATGCACCTTGGAGTCCTTGCAACCGCGTCTCATGCTGTTGCAACTCAAACTCTTTTTGTAGTATACTACAAGCCCAGGATGAGCCAGTTCATTATAAGTGTGAATAAGTATATGGAAGCTATCAACCAAAAATTTAGTGTTGGCATGAGGTTTAAGATGAGTTTTGATGGGGATGATTCTTCTGAGACTGACAAAAGATTTTCTGGCACAATAATTGGACAAGAGGATATTTCTTCACACTGGTTAAATTCAAAATGGAGATCACTCAAGGTTCAATGGGATGACGCTGCATCGATTCCAAGACCTGATAGAATTTCACCATGGGAGATAGAACCCCTTCTGGCTTCTGCTCCTACGACTATTCAACCTCCTGCTGCAAAATATAAAAGGCCTAGGCTACCTAGTGACGTTCCAGATTTTGGAGACACAACAGTAGGTGACTCTACTTACTGGGAAAATGGGCTGACACAATCCGATGTAACACAAGTTAATAATATTATGTCTGAAAACATAAGGAATGACGGTATGCATTTGTGGCATCATAAGCAAACTGATAATAGCAGCAGCAATGGTTTATCGAGGAATCTGACTGACGGAAGTTGGCTATCTTCTCCTCATTCAAGTGGTCCTTCTCATTTGTGTCAGGACACGACCGACGATAGCAAGAGTGTCGCAGTCTCGGCTTGGCCTATTTCAAAAACTCACTCAGAAATATTGAACAACAATGATGATTTGACTGATCAGGTTGTCAAGGAGAACAAAACGGAGGCTGCTACAAGCTGTAGATTGTTCGGGATTGATCTTAATGATCCTCGCACGAACACTCCTAAGGTGTCTGAGTATGCAGCAAATGTATTTGGAGTCACCTCTGAGGGATGCGTTGCCGCTCCCATGTCAAGAACTGATGCAGACCAAAATAAGTTTGATATCTCAAAGGCTTCAAAAGAAAGGAAACAAGAACAGCTACCGATATCTCCAAATGAAACACAAAGCAAGCAAACCAATAGCAGAAGTTGCACTAAGGTTCAAATGCAGGGTGTTGCAGTGGGTCGTGCGGTGGACTTGACTGCGTTGGATGGTTATGATCAACTTATTGATGAATTAGAGAAGCTGTTTGACATGAAGGGACAGATCCATCCTAGGAATAAATGGGAAATTGTCTTCACTGATGATGAAGGGGATATGATGCTTGTTGGTGATGATCCATGGCCGGAATTTTGCAATATGGTGAGAAGAATCTTCATTTGTTCCAGCCAAGATGTGCACAAAATGAGCTCAGGGACCAAATTACCTATCTCATCACTAGAAGAAACTGTAATAAGCTCAGACACAGCTGAGATATGA
Protein sequence

>MsaT037757.1

MMMNNGEENELYEQLWKACAGPHVEVPRAGQRVFYFPQGHMEQLEVSTNQELNQRIPLFKLPNKILCRVVNVHLLAEQETDEVYAQITLVPESNQTEPTSLDPCPAEPPRPRTHSFSKVLTNSDTSTHGGFSVLRKHATECLPSLDMSQSTPTQELVAKDLHNYEWHFKHIFRGQPRRHLLTTGWSTFVTSKRLVAGDTFVFLRGHNGELRVGVRHLASPQSCMPSSVISGQSMHLGVLATASHAVATQTLFVVYYKPRMSQFIISVNKYMEAINQKFSVGMRFKMSFDGDDSSETDKRFSGTIIGQEDISSHWLNSKWRSLKVQWDDAASIPRPDRISPWEIEPLLASAPTTIQPPAAKYKRPRLPSDVPDFGDTTVGDSTYWENGLTQSDVTQVNNIMSENIRNDGMHLWHHKQTDNSSSNGLSRNLTDGSWLSSPHSSGPSHLCQDTTDDSKSVAVSAWPISKTHSEILNNNDDLTDQVVKENKTEAATSCRLFGIDLNDPRTNTPKVSEYAANVFGVTSEGCVAAPMSRTDADQNKFDISKASKERKQEQLPISPNETQSKQTNSRSCTKVQMQGVAVGRAVDLTALDGYDQLIDELEKLFDMKGQIHPRNKWEIVFTDDEGDMMLVGDDPWPEFCNMVRRIFICSSQDVHKMSSGTKLPISSLEETVISSDTAEI*