AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG037846

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037846 MtrunA17_Chr7g0240371 95.946 518 18 2 1 517 1 516 0.0 1024
MsaG037846 MtrunA17_Chr4g0008591 50.329 608 201 18 2 517 3 601 4.29e-168 488
MsaG037846 MtrunA17_Chr4g0028701 41.606 411 167 11 125 467 231 636 1.39e-82 270
MsaG037846 MtrunA17_Chr2g0327021 47.719 285 109 7 126 378 235 511 1.50e-76 252
MsaG037846 MtrunA17_Chr8g0387321 35.535 439 215 12 139 514 185 618 5.00e-64 219
MsaG037846 MtrunA17_Chr8g0373801 44.444 288 123 7 135 393 384 663 2.29e-63 221
MsaG037846 MtrunA17_Chr5g0403251 41.333 300 138 7 115 393 329 611 9.90e-62 215
MsaG037846 MtrunA17_Chr1g0155681 39.869 306 149 6 143 425 174 467 2.27e-61 212
MsaG037846 MtrunA17_Chr4g0025521 43.772 281 120 7 139 393 217 485 1.14e-60 212
MsaG037846 MtrunA17_Chr3g0141941 40.909 264 128 6 135 376 158 415 5.28e-59 203
MsaG037846 MtrunA17_Chr1g0177751 44.674 291 132 7 138 407 160 442 8.58e-59 206
MsaG037846 MtrunA17_Chr3g0141931 41.264 269 134 6 136 384 162 426 9.72e-59 201
MsaG037846 MtrunA17_Chr7g0267481 52.551 196 83 4 201 390 250 441 2.06e-58 205
MsaG037846 MtrunA17_Chr1g0151681 32.394 284 166 8 136 398 240 518 7.25e-38 145
MsaG037846 MtrunA17_Chr5g0398181 37.778 90 51 2 295 379 201 290 3.39e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG037846 AT5G02030.1 47.992 498 190 16 72 517 95 575 9.93e-116 353
MsaG037846 AT2G27990.1 50.794 252 115 4 129 378 252 496 6.69e-76 250
MsaG037846 AT1G75410.1 46.792 265 109 9 134 376 162 416 5.63e-67 225
MsaG037846 AT1G75410.2 46.792 265 109 9 134 376 162 416 5.63e-67 225
MsaG037846 AT1G19700.1 46.097 269 110 7 135 376 161 421 8.83e-67 225
MsaG037846 AT1G19700.3 46.097 269 110 7 135 376 161 421 8.83e-67 225
MsaG037846 AT1G19700.5 46.097 269 110 7 135 376 161 421 8.83e-67 225
MsaG037846 AT1G19700.4 46.097 269 110 7 135 376 161 421 8.83e-67 225
MsaG037846 AT1G19700.2 46.097 269 110 7 135 376 161 421 8.83e-67 225
MsaG037846 AT2G16400.1 37.562 402 205 13 134 517 109 482 4.80e-66 221
MsaG037846 AT4G34610.2 45.161 248 115 5 143 376 144 384 1.61e-60 208
MsaG037846 AT4G34610.4 45.161 248 115 5 143 376 144 384 1.61e-60 208
MsaG037846 AT4G34610.3 45.161 248 115 5 143 376 144 384 1.61e-60 208
MsaG037846 AT4G34610.1 45.161 248 115 5 143 376 144 384 1.61e-60 208
MsaG037846 AT5G41410.1 35.160 438 203 16 140 516 194 611 3.73e-58 203
MsaG037846 AT4G36870.2 45.136 257 116 8 139 373 312 565 1.46e-57 204
MsaG037846 AT4G36870.1 45.136 257 116 8 139 373 312 565 1.46e-57 204
MsaG037846 AT2G27220.1 40.074 272 134 6 145 407 82 333 2.43e-57 197
MsaG037846 AT2G27220.3 40.074 272 134 6 145 407 82 333 2.43e-57 197
MsaG037846 AT2G23760.2 43.233 266 109 7 139 373 237 491 5.71e-57 201
MsaG037846 AT2G23760.1 43.233 266 109 7 139 373 237 491 5.71e-57 201
MsaG037846 AT2G23760.3 43.233 266 109 7 139 373 237 491 5.71e-57 201
MsaG037846 AT2G35940.3 41.197 284 129 6 117 367 168 446 3.55e-55 197
MsaG037846 AT2G35940.1 41.197 284 129 6 117 367 168 446 3.55e-55 197
MsaG037846 AT2G35940.2 41.197 284 129 6 117 367 168 446 3.55e-55 197
MsaG037846 AT2G27220.2 38.380 284 140 6 145 407 82 351 7.92e-54 188
MsaG037846 AT1G75410.3 44.215 242 103 9 134 353 162 393 9.61e-53 184
MsaG037846 AT1G75430.1 37.500 272 143 4 140 388 17 284 1.05e-52 181
MsaG037846 AT2G23760.4 35.873 315 114 7 139 373 237 543 1.26e-47 176
MsaG037846 AT2G27990.2 42.718 206 109 4 129 332 252 450 1.38e-44 163
MsaG037846 AT4G36870.4 41.597 238 114 8 139 354 312 546 9.85e-44 163
MsaG037846 AT4G36870.3 41.102 236 114 8 139 352 312 544 1.83e-42 159
MsaG037846 AT4G32980.1 31.579 304 165 8 95 373 154 439 8.34e-39 148
MsaG037846 AT4G32980.2 31.579 304 165 8 95 373 154 439 8.34e-39 148
MsaG037846 AT1G75430.2 30.556 216 123 4 140 332 17 228 4.17e-25 105
MsaG037846 AT1G23380.1 50.909 55 27 0 313 367 253 307 3.47e-11 65.1
MsaG037846 AT1G23380.2 50.909 55 27 0 313 367 255 309 3.55e-11 65.1
MsaG037846 AT1G62990.1 40.789 76 40 2 295 365 200 275 3.77e-11 64.7
MsaG037846 AT1G70510.1 50.909 55 27 0 313 367 237 291 8.67e-11 63.5
MsaG037846 AT1G70510.2 50.909 55 27 0 313 367 241 295 9.62e-11 63.5

Find 110 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACCTGCACAACAATTAT+TGG 0.152856 7:-66525993 MsaT037846.1:CDS
ACTTCATTTGAATATGTTTC+CGG 0.224546 7:-66524040 MsaT037846.1:CDS
CAATTGCAGAATAGGCAATT+TGG 0.234987 7:-66522706 MsaT037846.1:CDS
CTTCATCAAAATCATGGAAT+TGG 0.258172 7:-66522811 MsaT037846.1:CDS
CGCAGCTCCGTATGCAAGTT+TGG 0.286462 7:-66524008 MsaT037846.1:CDS
TTTGAATATGTTTCCGGCCT+TGG 0.297614 7:-66524034 MsaT037846.1:CDS
CTTCAAGAATCTAAAATGTT+TGG 0.306695 7:+66523970 None:intergenic
TCATCTCTCTGTGATTCCTT+TGG 0.313062 7:+66523051 None:intergenic
AGCGTGCTGTCAGTGTTCTT+AGG 0.318063 7:-66523775 MsaT037846.1:CDS
GTTCTGTTGTTGATGTTTCT+AGG 0.323329 7:-66526078 MsaT037846.1:CDS
TCTAGGAACACGGTTCCATT+AGG 0.325865 7:-66526061 MsaT037846.1:CDS
CCAATCCTAAACAGGTTTGC+AGG 0.327106 7:-66524102 MsaT037846.1:intron
GAAGGTAAAGGTGAATAGTT+AGG 0.327476 7:+66526313 None:intergenic
CAGCAGCACTCATCTGATTC+TGG 0.330179 7:+66526116 None:intergenic
GTTCCATTAGGGCCATTTAC+AGG 0.333535 7:-66526049 MsaT037846.1:CDS
CCACTAGCGGCGTAACTATC+AGG 0.342608 7:+66522733 None:intergenic
ACAGAAGCATAACCTGTAAA+TGG 0.347909 7:+66526037 None:intergenic
AATTGCAGAATAGGCAATTT+GGG 0.349127 7:-66522705 MsaT037846.1:CDS
AAGATTCAATGAAACTTGTT+TGG 0.360190 7:+66526350 None:intergenic
ATGTGTTACAGGTGTCTAAT+TGG 0.376653 7:-66523146 MsaT037846.1:intron
GCGTGCTGTCAGTGTTCTTA+GGG 0.378409 7:-66523774 MsaT037846.1:CDS
GATGATAAAGACAAAGATAA+AGG 0.380053 7:+66526151 None:intergenic
ATGAGTGCCGCACAGCGTTT+TGG 0.383305 7:-66522769 MsaT037846.1:CDS
TAACCTGTAAATGGCCCTAA+TGG 0.383820 7:+66526046 None:intergenic
GGTAACAGTGGAATGATTAA+TGG 0.384759 7:-66525893 MsaT037846.1:CDS
TGCCGTTGATGCATCTTTGA+TGG 0.387842 7:-66525927 MsaT037846.1:CDS
TTTCTCTTCTGCTTTGTTGT+GGG 0.388583 7:+66526395 None:intergenic
TTTGAGTTTGATAAAGAAAA+GGG 0.396823 7:+66526242 None:intergenic
TAAATGCAAGAGTAAGGCTT+TGG 0.400143 7:-66523119 MsaT037846.1:CDS
CCTCTTTGAGGTCGCCAAAC+AGG 0.409862 7:+66523806 None:intergenic
TTCATCAAAATCATGGAATT+GGG 0.411246 7:-66522810 MsaT037846.1:CDS
AACCCTGGCCTTTGACTGTA+AGG 0.418297 7:+66523848 None:intergenic
CTAGGAACACGGTTCCATTA+GGG 0.419262 7:-66526060 MsaT037846.1:CDS
GGTTTATAAATGCAAGAGTA+AGG 0.420041 7:-66523125 MsaT037846.1:CDS
TCATCCAATAATTGTTGTGC+AGG 0.423901 7:+66525989 None:intergenic
ATTTGAGTTTGATAAAGAAA+AGG 0.427297 7:+66526241 None:intergenic
CGCTCAGGGAGCCCTCTTTG+AGG 0.433129 7:+66523794 None:intergenic
ACTGACCAGCTTCAGTATAT+CGG 0.436419 7:-66523938 MsaT037846.1:CDS
AGGTGAATAGTTAGGGTTAA+GGG 0.439612 7:+66526321 None:intergenic
CTAATGTTGGCTAAACAAAC+TGG 0.446507 7:-66523269 MsaT037846.1:CDS
CTTGGTCTTCATCAAAATCA+TGG 0.447479 7:-66522817 MsaT037846.1:CDS
AAGGTGAATAGTTAGGGTTA+AGG 0.455170 7:+66526320 None:intergenic
AACATGTCCAACAACCTGTT+TGG 0.456323 7:-66523820 MsaT037846.1:CDS
AACTTGTCTGTGGAGGTAGA+TGG 0.462590 7:+66522985 None:intergenic
AATCAACAAGTTGGTAATGT+TGG 0.479434 7:-66522889 MsaT037846.1:CDS
GTTTAGTACTATGGCTGATG+AGG 0.481895 7:-66526440 None:intergenic
AAGGTAAAGGTGAATAGTTA+GGG 0.487879 7:+66526314 None:intergenic
GCCCCTTACAGTCAAAGGCC+AGG 0.493461 7:-66523851 MsaT037846.1:CDS
AGAACACTGACAGCACGCTC+AGG 0.494420 7:+66523779 None:intergenic
TTGCTTGCGATACTATGATT+CGG 0.499860 7:+66522910 None:intergenic
GAGGTCGCCAAACAGGTTGT+TGG 0.502990 7:+66523813 None:intergenic
TTTAGTACTATGGCTGATGA+GGG 0.503174 7:-66526439 None:intergenic
TTACTGTTTATAGCATCTAA+AGG 0.509725 7:+66526265 None:intergenic
GATGATACACTTGGTGATGA+TGG 0.511653 7:-66525863 MsaT037846.1:CDS
ATGTGTATTTCCTCCACCAT+TGG 0.521327 7:+66523093 None:intergenic
CGGAGCTGCGTTACCAAGGC+CGG 0.523680 7:+66524021 None:intergenic
ATTAGCTTGTCAGTATCAGT+AGG 0.534775 7:+66523287 None:intergenic
CCATCGCCGTTGTGAAACCT+TGG 0.538655 7:+66523875 None:intergenic
TTATCAGATAAGCAATAGAA+AGG 0.539123 7:-66523906 MsaT037846.1:CDS
CCAAGGTTTCACAACGGCGA+TGG 0.539915 7:-66523875 MsaT037846.1:CDS
ATCGCAAGCAATCAACAAGT+TGG 0.542496 7:-66522898 MsaT037846.1:CDS
CTGTCAGTGTTCTTAGGGGA+TGG 0.544619 7:-66523769 MsaT037846.1:CDS
CCCTGGCCTTTGACTGTAAG+GGG 0.546356 7:+66523850 None:intergenic
GGAGTGAGCATGATGAATAA+TGG 0.547619 7:-66522868 MsaT037846.1:CDS
ATGGAGCCCCTTACAGTCAA+AGG 0.556822 7:-66523856 MsaT037846.1:CDS
ACTTGCCGATATACTGAAGC+TGG 0.560095 7:+66523933 None:intergenic
AGTGAAGGATCGTAAAGAGA+AGG 0.562891 7:+66526295 None:intergenic
GGCACTCATGTTGAAAGGCT+CGG 0.566451 7:+66522783 None:intergenic
GGATCGTAAAGAGAAGGTAA+AGG 0.570346 7:+66526301 None:intergenic
TTGGATGAGATATGTGATGT+TGG 0.571767 7:-66525974 MsaT037846.1:CDS
TTTATAGCCAAACTTGCATA+CGG 0.572894 7:+66524001 None:intergenic
TGATACTGACAAGCTAATGT+TGG 0.576970 7:-66523282 MsaT037846.1:CDS
AACTGGTCTATCTCGTAACC+AGG 0.577110 7:-66523252 MsaT037846.1:intron
CCTGATAGTTACGCCGCTAG+TGG 0.577261 7:-66522733 MsaT037846.1:CDS
TCTTTGATGGAAACTAATCA+TGG 0.579826 7:-66525914 MsaT037846.1:CDS
TGGACATGTTCTACAAACCC+TGG 0.582558 7:+66523833 None:intergenic
GCTTTGGAAGCCAATGGTGG+AGG 0.582607 7:-66523103 MsaT037846.1:CDS
GAACACTGACAGCACGCTCA+GGG 0.586445 7:+66523780 None:intergenic
TGTTGATGTTTCTAGGAACA+CGG 0.590452 7:-66526071 MsaT037846.1:CDS
TTCCATCAAAGATGCATCAA+CGG 0.593348 7:+66525925 None:intergenic
TGTGCGGCACTCATGTTGAA+AGG 0.596915 7:+66522778 None:intergenic
CTGTTTGGCGACCTCAAAGA+GGG 0.600827 7:-66523805 MsaT037846.1:CDS
AATGGTGTATCTCTGACGCT+TGG 0.601191 7:-66522835 MsaT037846.1:CDS
GAATCTAAAATGTTTGGACA+TGG 0.606211 7:+66523976 None:intergenic
CCCCTTACAGTCAAAGGCCA+GGG 0.608843 7:-66523850 MsaT037846.1:CDS
GAAACTAATCATGGTAACAG+TGG 0.611291 7:-66525905 MsaT037846.1:CDS
AATAATGGTGCAACAAGCAA+TGG 0.619469 7:-66522853 MsaT037846.1:CDS
TGAATAGTTAGGGTTAAGGG+TGG 0.619527 7:+66526324 None:intergenic
AGTAAGGCTTTGGAAGCCAA+TGG 0.623632 7:-66523109 MsaT037846.1:CDS
ATCGATAAACTTGTCTGTGG+AGG 0.624502 7:+66522978 None:intergenic
ACCCTGGCCTTTGACTGTAA+GGG 0.626110 7:+66523849 None:intergenic
CCTGTTTGGCGACCTCAAAG+AGG 0.626862 7:-66523806 MsaT037846.1:CDS
CATACGGAGCTGCGTTACCA+AGG 0.627780 7:+66524017 None:intergenic
TGAATCACAGCAATCTCCAA+AGG 0.631385 7:-66523067 MsaT037846.1:CDS
ATTGTTAACAGTGCTTGATG+AGG 0.636658 7:-66525822 MsaT037846.1:intron
TCTAAAGGTGAAATGAGTGA+AGG 0.639475 7:+66526280 None:intergenic
ATACACTTGGTGATGATGGA+AGG 0.640811 7:-66525859 MsaT037846.1:CDS
AAGGCTTTGGAAGCCAATGG+TGG 0.647272 7:-66523106 MsaT037846.1:CDS
ATAGAAAGGATGAATCTCCA+AGG 0.653144 7:-66523892 MsaT037846.1:CDS
ATGAATCATGTATACAACGA+TGG 0.656023 7:-66526220 MsaT037846.1:CDS
CGTGCTGTCAGTGTTCTTAG+GGG 0.662282 7:-66523773 MsaT037846.1:CDS
CTGCAATTGAAAACCACTAG+CGG 0.669466 7:+66522720 None:intergenic
TTAGCTTGTCAGTATCAGTA+GGG 0.670053 7:+66523288 None:intergenic
TGCAAGACCAAAACGCTGTG+CGG 0.675306 7:+66522762 None:intergenic
ATCCTAAACAGGTTTGCAGG+AGG 0.676212 7:-66524099 MsaT037846.1:intron
GAATAGTTAGGGTTAAGGGT+GGG 0.680467 7:+66526325 None:intergenic
AATCATGTATACAACGATGG+TGG 0.691205 7:-66526217 MsaT037846.1:CDS
GGTGTTGATGATGATACACT+TGG 0.700349 7:-66525872 MsaT037846.1:CDS
AACATCGATAAACTTGTCTG+TGG 0.700915 7:+66522975 None:intergenic
GAATCTCCAAGGTTTCACAA+CGG 0.755483 7:-66523881 MsaT037846.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 66522660 66526452 66522660 ID=MsaG037846
Chr7 mRNA 66522660 66526452 66522660 ID=MsaT037846.1;Parent=MsaG037846
Chr7 exon 66522660 66523157 66522660 ID=MsaT037846.1.exon4;Parent=MsaT037846.1
Chr7 CDS 66522660 66523157 66522660 ID=cds.MsaT037846.1;Parent=MsaT037846.1
Chr7 exon 66523253 66523313 66523253 ID=MsaT037846.1.exon3;Parent=MsaT037846.1
Chr7 CDS 66523253 66523313 66523253 ID=cds.MsaT037846.1;Parent=MsaT037846.1
Chr7 exon 66523746 66524110 66523746 ID=MsaT037846.1.exon2;Parent=MsaT037846.1
Chr7 CDS 66523746 66524110 66523746 ID=cds.MsaT037846.1;Parent=MsaT037846.1
Chr7 exon 66525823 66526452 66525823 ID=MsaT037846.1.exon1;Parent=MsaT037846.1
Chr7 CDS 66525823 66526452 66525823 ID=cds.MsaT037846.1;Parent=MsaT037846.1
Gene Sequence

>MsaT037846.1

ATGGCTGATGAGGGTTTTGAAAATTATTATCATATCCCACAACAAAGCAGAAGAGAAAAACTTCGATATTTATCTCAAAACCAAACAAGTTTCATTGAATCTTCACCCACCCTTAACCCTAACTATTCACCTTTACCTTCTCTTTACGATCCTTCACTCATTTCACCTTTAGATGCTATAAACAGTAACCCTTTTCTTTATCAAACTCAAATGAATCATGTATACAACGATGGTGGTAGTAGTAGTAACAACAATGAAGTTATGTTGTTGAAATCTGAACCTTTATCTTTGTCTTTATCATCAAACAAAAACAACCAGAATCAGATGAGTGCTGCTGATTTTCAGCGTTATGGTTCTGTTGTTGATGTTTCTAGGAACACGGTTCCATTAGGGCCATTTACAGGTTATGCTTCTGTTTTGAAGGGATCGAGGTTTTTGAAACCTGCACAACAATTATTGGATGAGATATGTGATGTTGGTGTTCGTGCTGAGAAGATTATTGCTGCCGTTGATGCATCTTTGATGGAAACTAATCATGGTAACAGTGGAATGATTAATGGTGTTGATGATGATACACTTGGTGATGATGGAAGGAAGAACAAGTCTAGATTGTTAACAGTGCTTGATGAGGTTTGCAGGAGGTACAGACAGTACTATCAGCAGATTCATGCAGTAATAACTTCATTTGAATATGTTTCCGGCCTTGGTAACGCAGCTCCGTATGCAAGTTTGGCTATAAATGCCATGTCCAAACATTTTAGATTCTTGAAGAATGTGATTACTGACCAGCTTCAGTATATCGGCAAGTCTAATTATCAGATAAGCAATAGAAAGGATGAATCTCCAAGGTTTCACAACGGCGATGGAGCCCCTTACAGTCAAAGGCCAGGGTTTGTAGAACATGTCCAACAACCTGTTTGGCGACCTCAAAGAGGGCTCCCTGAGCGTGCTGTCAGTGTTCTTAGGGGATGGTTGTTTGAACACTTTTTGCATCCTTACCCTACTGATACTGACAAGCTAATGTTGGCTAAACAAACTGGTCTATCTCGTAACCAGGTGTCTAATTGGTTTATAAATGCAAGAGTAAGGCTTTGGAAGCCAATGGTGGAGGAAATACACATGCTTGAATCACAGCAATCTCCAAAGGAATCACAGAGAGATGAACATAGTCGAAACAATTTAAGCGAGAACAACATTGCTGAGAATCCATCTACCTCCACAGACAAGTTTATCGATGTTGCATATAAACGCACTAGAAACGAACTTCACAACATGTCAGTGCCGAATCATAGTATCGCAAGCAATCAACAAGTTGGTAATGTTGGAGTGAGCATGATGAATAATGGTGCAACAAGCAATGGTGTATCTCTGACGCTTGGTCTTCATCAAAATCATGGAATTGGGTTATCCGAGCCTTTCAACATGAGTGCCGCACAGCGTTTTGGTCTTGCACATCAACCTGATAGTTACGCCGCTAGTGGTTTTCAATTGCAGAATAGGCAATTTGGGAGAGATTTTATTGGTGGGCAACTTTTGCGTGACTATGTAGGATGA
Protein sequence

>MsaT037846.1

MADEGFENYYHIPQQSRREKLRYLSQNQTSFIESSPTLNPNYSPLPSLYDPSLISPLDAINSNPFLYQTQMNHVYNDGGSSSNNNEVMLLKSEPLSLSLSSNKNNQNQMSAADFQRYGSVVDVSRNTVPLGPFTGYASVLKGSRFLKPAQQLLDEICDVGVRAEKIIAAVDASLMETNHGNSGMINGVDDDTLGDDGRKNKSRLLTVLDEVCRRYRQYYQQIHAVITSFEYVSGLGNAAPYASLAINAMSKHFRFLKNVITDQLQYIGKSNYQISNRKDESPRFHNGDGAPYSQRPGFVEHVQQPVWRPQRGLPERAVSVLRGWLFEHFLHPYPTDTDKLMLAKQTGLSRNQVSNWFINARVRLWKPMVEEIHMLESQQSPKESQRDEHSRNNLSENNIAENPSTSTDKFIDVAYKRTRNELHNMSVPNHSIASNQQVGNVGVSMMNNGATSNGVSLTLGLHQNHGIGLSEPFNMSAAQRFGLAHQPDSYAASGFQLQNRQFGRDFIGGQLLRDYVG*