AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038061


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038061 MtrunA17_Chr7g0242511 100.000 242 0 0 1 242 1 242 0.0 503
MsaG038061 MtrunA17_Chr4g0006381 90.984 244 18 3 1 242 1 242 1.83e-144 403
MsaG038061 MtrunA17_Chr4g0076771 61.355 251 87 4 1 241 4 254 1.03e-91 270
MsaG038061 MtrunA17_Chr8g0343441 57.831 249 93 5 6 242 8 256 4.10e-88 261
MsaG038061 MtrunA17_Chr1g0150821 59.459 259 79 5 6 242 9 263 7.55e-85 253
MsaG038061 MtrunA17_Chr2g0300241 56.538 260 88 6 1 242 1 253 9.71e-85 253
MsaG038061 MtrunA17_Chr2g0329821 56.557 244 96 4 3 236 1 244 5.81e-82 245
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038061 AT5G05610.2 73.950 238 60 1 4 241 5 240 1.34e-116 333
MsaG038061 AT5G05610.1 73.950 238 60 1 4 241 5 240 1.34e-116 333
MsaG038061 AT3G11200.1 77.083 240 54 1 3 241 6 245 3.41e-115 330
MsaG038061 AT3G11200.2 75.481 208 50 1 35 241 25 232 1.83e-97 284
MsaG038061 AT3G42790.1 62.397 242 84 5 6 241 9 249 7.96e-93 273
MsaG038061 AT5G20510.1 59.127 252 88 4 6 242 9 260 7.77e-88 261
MsaG038061 AT2G02470.1 60.484 248 86 4 7 242 9 256 8.39e-88 261
MsaG038061 AT2G02470.2 59.336 241 91 3 7 242 9 247 2.22e-85 254
MsaG038061 AT1G14510.1 58.333 240 93 4 7 239 9 248 1.35e-82 247
MsaG038061 AT5G26210.1 57.430 249 95 4 4 241 6 254 3.03e-77 234
MsaG038061 AT5G20510.2 59.722 216 72 4 42 242 4 219 4.36e-69 212
MsaG038061 AT5G20510.3 61.875 160 56 3 6 160 9 168 1.73e-55 176
MsaG038061 AT2G02470.3 62.179 156 57 1 7 160 9 164 5.76e-55 177

Find 68 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGCTTGTTGTCCTTAAT+TGG 0.215056 7:-70499853 None:intergenic
ATTACAATGCCGACGAGTTT+TGG 0.256361 7:+70500108 MsaT038061.1:CDS
CTTTCTGTGGCGTTCTATCT+TGG 0.287893 7:+70498468 MsaT038061.1:CDS
TTGCTGTGCACAGTGATTCA+TGG 0.330962 7:+70498442 MsaT038061.1:CDS
AAGGATAATTTGTGTCTCTA+TGG 0.357930 7:+70498294 MsaT038061.1:CDS
CTTGTATTGCTTTATGCTCT+CGG 0.362549 7:-70500195 None:intergenic
GACCGACAGGAAACCAATTA+AGG 0.374595 7:+70499840 MsaT038061.1:CDS
TTGCTTCCACTGTCCGCTGC+TGG 0.382118 7:-70499871 None:intergenic
CCGTCATCCACAGGAAACTT+TGG 0.383878 7:-70500026 None:intergenic
AGAGCTGGCTCAGGGAGTTC+CGG 0.393745 7:-70498366 None:intergenic
TCTCAATTGGGTTATGGAAA+TGG 0.397199 7:+70497554 None:intergenic
CACTAACCTTGGTGCTTCCT+CGG 0.397306 7:-70499897 None:intergenic
TTGCTTTATGCTCTCGGCCT+TGG 0.413678 7:-70500189 None:intergenic
CGTCATCCACAGGAAACTTT+GGG 0.432337 7:-70500025 None:intergenic
GAACTCCCTGAGCCAGCTCT+TGG 0.435136 7:+70498369 MsaT038061.1:CDS
TCCTGTGGATGACGGTTATG+AGG 0.442634 7:+70500034 MsaT038061.1:CDS
AGCACAGTGAAACACTTTGC+GGG 0.443628 7:+70500072 MsaT038061.1:CDS
TCCGCCTGATGCTGCATTGA+GGG 0.446153 7:-70500220 None:intergenic
TTTATGCTCTCGGCCTTGGC+AGG 0.451134 7:-70500185 None:intergenic
TCCGGTGGAACTTCCTCTGC+TGG 0.464747 7:-70498348 None:intergenic
AGTGCCCTCAATGCAGCATC+AGG 0.465718 7:+70500216 MsaT038061.1:CDS
ACTAACCTTGGTGCTTCCTC+GGG 0.472237 7:-70499896 None:intergenic
TTGATTCCAAGAGCTGGCTC+AGG 0.473652 7:-70498375 None:intergenic
ATGGTCATAATAATGAAAGC+TGG 0.475692 7:+70498313 MsaT038061.1:CDS
ACGGTATGAACCGTAGAGAT+TGG 0.485613 7:+70498409 MsaT038061.1:CDS
GTCGGTCACAATTTCGAAAA+CGG 0.493814 7:-70499824 None:intergenic
GTCCTTAATTGGTTTCCTGT+CGG 0.494680 7:-70499842 None:intergenic
TTCCGCCTGATGCTGCATTG+AGG 0.510891 7:-70500221 None:intergenic
TGACGGTTATGAGGAGGAGG+AGG 0.514089 7:+70500043 MsaT038061.1:CDS
GCAAAATTGATTCCAAGAGC+TGG 0.521530 7:-70498381 None:intergenic
TCCTCATAACCGTCATCCAC+AGG 0.530396 7:-70500035 None:intergenic
TGGTCATAATAATGAAAGCT+GGG 0.544113 7:+70498314 MsaT038061.1:CDS
GTTGTTCGTGCTCTCACTCA+AGG 0.552840 7:+70497636 MsaT038061.1:CDS
CAAGAGAAAGCCAATCTCTA+CGG 0.553772 7:-70498419 None:intergenic
ATTCGCAGAGATCCAGTGAC+GGG 0.560137 7:+70499988 MsaT038061.1:intron
GCCAGCAGAGGAAGTTCCAC+CGG 0.563034 7:+70498347 MsaT038061.1:CDS
AAGATCTCTTCAACGGTACG+AGG 0.564667 7:-70497585 None:intergenic
GAAACACTTTGCGGGAGCTG+TGG 0.565640 7:+70500080 MsaT038061.1:CDS
TGATTCATGGTTGCTTTCTG+TGG 0.570084 7:+70498455 MsaT038061.1:CDS
ACACTTTGCGGGAGCTGTGG+CGG 0.573976 7:+70500083 MsaT038061.1:CDS
ATCTTTGAAGATCTCTTCAA+CGG 0.581501 7:-70497592 None:intergenic
CCAAAGTTTCCTGTGGATGA+CGG 0.582757 7:+70500026 MsaT038061.1:CDS
GAGCACAGTGAAACACTTTG+CGG 0.584136 7:+70500071 MsaT038061.1:CDS
GGATGACGGTTATGAGGAGG+AGG 0.591224 7:+70500040 MsaT038061.1:CDS
CTCGTCTTAACCGCAATGAA+AGG 0.595014 7:+70498493 MsaT038061.1:CDS
TATGTGAGAGGTGGTACCAT+GGG 0.596316 7:+70500144 MsaT038061.1:CDS
GTCACAATTTCGAAAACGGT+GGG 0.600381 7:-70499820 None:intergenic
TGTGAAGATCACTCCTGCCA+AGG 0.609845 7:+70500172 MsaT038061.1:CDS
AACAAGCCAGCAGCGGACAG+TGG 0.616447 7:+70499865 MsaT038061.1:CDS
GCTGGCTCAGGGAGTTCCGG+TGG 0.616538 7:-70498363 None:intergenic
TGATCTTCACACATTTCCCA+TGG 0.618512 7:-70500160 None:intergenic
TGATTCCAAGAGCTGGCTCA+GGG 0.623716 7:-70498374 None:intergenic
GGAAGTAACTCTGCCAGCAG+AGG 0.636663 7:+70498335 MsaT038061.1:CDS
GCCCTCAATGCAGCATCAGG+CGG 0.644933 7:+70500219 MsaT038061.1:CDS
TTGAGTGAGAGCACGAACAA+CGG 0.655872 7:-70497634 None:intergenic
GGTCACAATTTCGAAAACGG+TGG 0.656142 7:-70499821 None:intergenic
AAGCAACCCAAAGTTTCCTG+TGG 0.656897 7:+70500019 MsaT038061.1:CDS
TTGGTTGTGATATATGTGAG+AGG 0.665767 7:+70500132 MsaT038061.1:CDS
TGTGGATGACGGTTATGAGG+AGG 0.666323 7:+70500037 MsaT038061.1:CDS
ACAACCAATCCAAAACTCGT+CGG 0.669542 7:-70500117 None:intergenic
AGATCTCTTCAACGGTACGA+GGG 0.670323 7:-70497584 None:intergenic
TATTCGCAGAGATCCAGTGA+CGG 0.673421 7:+70499987 MsaT038061.1:intron
TAAGGACAACAAGCCAGCAG+CGG 0.678184 7:+70499858 MsaT038061.1:CDS
ATAGAATGTACCTTTCATTG+CGG 0.683230 7:-70498503 None:intergenic
GACAGTGGAAGCAAATCCCG+AGG 0.687670 7:+70499880 MsaT038061.1:CDS
ATATGTGAGAGGTGGTACCA+TGG 0.697894 7:+70500143 MsaT038061.1:CDS
CAAATCCCGAGGAAGCACCA+AGG 0.723813 7:+70499891 MsaT038061.1:CDS
GTTGTGATATATGTGAGAGG+TGG 0.732743 7:+70500135 MsaT038061.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 70497567 70500253 70497567 ID=MsaG038061
Chr7 mRNA 70497567 70500253 70497567 ID=MsaT038061.1;Parent=MsaG038061
Chr7 exon 70497567 70497657 70497567 ID=MsaT038061.1.exon1;Parent=MsaT038061.1
Chr7 CDS 70497567 70497657 70497567 ID=cds.MsaT038061.1;Parent=MsaT038061.1
Chr7 exon 70497764 70497796 70497764 ID=MsaT038061.1.exon2;Parent=MsaT038061.1
Chr7 CDS 70497764 70497796 70497764 ID=cds.MsaT038061.1;Parent=MsaT038061.1
Chr7 exon 70498292 70498514 70498292 ID=MsaT038061.1.exon3;Parent=MsaT038061.1
Chr7 CDS 70498292 70498514 70498292 ID=cds.MsaT038061.1;Parent=MsaT038061.1
Chr7 exon 70499789 70499912 70499789 ID=MsaT038061.1.exon4;Parent=MsaT038061.1
Chr7 CDS 70499789 70499912 70499789 ID=cds.MsaT038061.1;Parent=MsaT038061.1
Chr7 exon 70499996 70500253 70499996 ID=MsaT038061.1.exon5;Parent=MsaT038061.1
Chr7 CDS 70499996 70500253 70499996 ID=cds.MsaT038061.1;Parent=MsaT038061.1
Gene Sequence

>MsaT038061.1

ATGGAAATGGCTTCTAGCCCTCGTACCGTTGAAGAGATCTTCAAAGATTACAGCGCTCGTAGAATCGCCGTTGTTCGTGCTCTCACTCAAGATGTTGATGAATTTTACGGTCTCTGTGATCCAGATAAGGATAATTTGTGTCTCTATGGTCATAATAATGAAAGCTGGGAAGTAACTCTGCCAGCAGAGGAAGTTCCACCGGAACTCCCTGAGCCAGCTCTTGGAATCAATTTTGCTCGAGACGGTATGAACCGTAGAGATTGGCTTTCTCTTGTTGCTGTGCACAGTGATTCATGGTTGCTTTCTGTGGCGTTCTATCTTGGAGCTCGTCTTAACCGCAATGAAAGGAAACGTTTGTTTAGTTTGATCAACGAACTTCCCACCGTTTTCGAAATTGTGACCGACAGGAAACCAATTAAGGACAACAAGCCAGCAGCGGACAGTGGAAGCAAATCCCGAGGAAGCACCAAGAGATCCAGTGACGGGCAAGTCAAAAGCAACCCAAAGTTTCCTGTGGATGACGGTTATGAGGAGGAGGAGGAAGACGAGCACAGTGAAACACTTTGCGGGAGCTGTGGCGGTAATTACAATGCCGACGAGTTTTGGATTGGTTGTGATATATGTGAGAGGTGGTACCATGGGAAATGTGTGAAGATCACTCCTGCCAAGGCCGAGAGCATAAAGCAATACAAGTGCCCTCAATGCAGCATCAGGCGGAACAGACCCTAG

Protein sequence

>MsaT038061.1

MEMASSPRTVEEIFKDYSARRIAVVRALTQDVDEFYGLCDPDKDNLCLYGHNNESWEVTLPAEEVPPELPEPALGINFARDGMNRRDWLSLVAVHSDSWLLSVAFYLGARLNRNERKRLFSLINELPTVFEIVTDRKPIKDNKPAADSGSKSRGSTKRSSDGQVKSNPKFPVDDGYEEEEEDEHSETLCGSCGGNYNADEFWIGCDICERWYHGKCVKITPAKAESIKQYKCPQCSIRRNRP*