AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038062


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038062 MtrunA17_Chr7g0242521 92.017 238 18 1 1 238 1 237 9.33e-162 447
MsaG038062 MtrunA17_Chr7g0254981 55.303 132 53 2 13 141 50 178 1.38e-43 148
MsaG038062 MtrunA17_Chr2g0291021 48.408 157 73 5 13 166 49 200 4.17e-42 145
MsaG038062 MtrunA17_Chr1g0175091 49.275 138 64 4 2 138 246 378 2.06e-35 130
MsaG038062 MtrunA17_Chr7g0261911 49.306 144 58 4 1 138 250 384 1.78e-34 128
MsaG038062 MtrunA17_Chr7g0258551 46.512 129 65 2 13 141 212 336 1.01e-33 125
MsaG038062 MtrunA17_Chr1g0190301 43.871 155 79 5 13 166 175 322 2.06e-32 121
MsaG038062 MtrunA17_Chr2g0310011 45.038 131 66 2 13 140 35 162 3.77e-29 112
MsaG038062 MtrunA17_Chr2g0321461 41.611 149 64 3 13 141 45 190 4.63e-29 112
MsaG038062 MtrunA17_Chr6g0461641 41.611 149 68 3 9 141 38 183 8.40e-29 112
MsaG038062 MtrunA17_Chr5g0409731 41.958 143 64 3 13 141 25 162 2.44e-28 108
MsaG038062 MtrunA17_Chr7g0274471 42.636 129 71 1 13 141 218 343 3.42e-28 110
MsaG038062 MtrunA17_Chr8g0356501 42.424 132 70 2 13 141 37 165 2.84e-27 107
MsaG038062 MtrunA17_Chr4g0042451 39.310 145 72 4 9 141 13 153 4.72e-26 103
MsaG038062 MtrunA17_Chr5g0417521 37.415 147 73 4 9 141 11 152 2.31e-24 99.4
MsaG038062 MtrunA17_Chr2g0290991 33.333 180 95 3 9 176 35 201 1.03e-23 97.1
MsaG038062 MtrunA17_Chr1g0175071 42.754 138 73 3 3 138 165 298 6.98e-23 94.7
MsaG038062 MtrunA17_Chr1g0197171 35.897 156 78 3 13 159 44 186 5.15e-21 89.4
MsaG038062 MtrunA17_Chr7g0258531 35.404 161 88 5 1 153 201 353 3.55e-17 79.3
MsaG038062 MtrunA17_Chr1g0175081 45.238 84 42 2 9 92 18 97 1.85e-16 72.4
MsaG038062 MtrunA17_Chr2g0320231 59.322 59 21 1 15 73 99 154 4.12e-13 67.8
MsaG038062 MtrunA17_Chr3g0095611 49.296 71 30 2 2 69 5 72 4.30e-13 66.2
MsaG038062 MtrunA17_Chr8g0379121 57.627 59 22 1 15 73 113 168 6.33e-13 67.4
MsaG038062 MtrunA17_Chr7g0243081 55.000 60 24 1 15 74 73 129 8.15e-13 67.0
MsaG038062 MtrunA17_Chr6g0479431 53.448 58 24 1 15 72 204 258 2.15e-12 65.9
MsaG038062 MtrunA17_Chr2g0288601 61.538 52 17 1 15 66 73 121 2.40e-12 62.4
MsaG038062 MtrunA17_Chr8g0366281 52.542 59 25 1 15 73 175 230 4.44e-12 64.7
MsaG038062 MtrunA17_Chr1g0154261 43.038 79 42 1 15 93 128 203 6.82e-12 63.9
MsaG038062 MtrunA17_Chr3g0109981 39.773 88 50 1 15 102 276 360 7.91e-12 64.3
MsaG038062 MtrunA17_Chr5g0410741 53.448 58 24 1 15 72 221 275 2.05e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038062 AT2G01060.1 46.951 164 79 4 9 167 11 171 8.10e-45 152
MsaG038062 AT5G06800.1 48.551 138 67 2 9 146 188 321 3.17e-37 134
MsaG038062 AT5G06800.2 47.183 142 68 2 9 146 188 326 9.21e-37 133
MsaG038062 AT1G79430.2 43.506 154 68 4 13 161 34 173 1.06e-32 122
MsaG038062 AT4G28610.1 47.794 136 57 3 9 138 221 348 1.58e-32 122
MsaG038062 AT5G29000.1 43.571 140 73 3 2 138 177 313 1.49e-31 119
MsaG038062 AT5G29000.3 43.571 140 73 3 2 138 177 313 1.49e-31 119
MsaG038062 AT5G29000.2 43.571 140 73 3 2 138 220 356 2.01e-31 119
MsaG038062 AT1G69580.1 43.709 151 63 4 9 141 30 176 6.87e-31 117
MsaG038062 AT1G69580.2 43.421 152 63 4 9 141 30 177 1.02e-30 116
MsaG038062 AT3G24120.1 33.036 224 120 5 13 219 41 251 3.92e-30 114
MsaG038062 AT3G04450.4 38.596 171 92 5 9 170 194 360 1.46e-29 114
MsaG038062 AT3G04450.2 38.596 171 92 5 9 170 194 360 1.46e-29 114
MsaG038062 AT3G04450.1 38.596 171 92 5 9 170 234 400 1.94e-29 114
MsaG038062 AT4G13640.1 37.288 177 83 4 13 175 37 199 1.32e-28 109
MsaG038062 AT3G13040.3 42.857 147 75 3 1 141 228 371 2.93e-28 111
MsaG038062 AT3G13040.1 42.857 147 75 3 1 141 228 371 2.93e-28 111
MsaG038062 AT3G13040.2 42.857 147 75 3 1 141 228 371 2.93e-28 111
MsaG038062 AT3G24120.2 32.599 227 120 6 13 219 41 254 4.77e-28 108
MsaG038062 AT3G04030.3 42.282 149 66 4 9 141 41 185 8.26e-28 109
MsaG038062 AT5G18240.1 44.138 145 61 5 13 141 45 185 9.19e-28 109
MsaG038062 AT5G18240.4 44.138 145 61 5 13 141 45 185 9.19e-28 109
MsaG038062 AT2G20400.2 43.307 127 68 2 13 138 231 354 1.38e-26 106
MsaG038062 AT4G13640.2 36.667 180 83 5 13 175 37 202 1.51e-26 104
MsaG038062 AT2G01060.2 43.443 122 64 3 51 167 1 122 8.20e-26 101
MsaG038062 AT2G20400.1 41.270 126 71 1 13 138 231 353 2.11e-25 103
MsaG038062 AT3G04030.2 41.611 149 66 5 9 141 41 184 3.42e-25 102
MsaG038062 AT5G45580.1 38.194 144 68 3 13 138 7 147 7.35e-25 99.4
MsaG038062 AT5G45580.2 38.194 144 68 3 13 138 23 163 1.18e-24 99.4
MsaG038062 AT5G18240.5 42.759 145 61 6 13 141 45 183 1.46e-24 100
MsaG038062 AT3G12730.1 37.778 135 73 3 13 139 23 154 1.30e-23 95.5
MsaG038062 AT3G04030.1 40.268 149 63 5 9 141 41 179 2.73e-23 97.1
MsaG038062 AT5G18240.2 42.069 145 58 6 13 141 45 179 3.63e-23 97.1
MsaG038062 AT5G18240.3 42.069 145 58 6 13 141 45 179 3.63e-23 97.1
MsaG038062 AT5G06800.3 62.319 69 23 1 9 77 188 253 1.59e-22 93.6
MsaG038062 AT5G29000.4 47.872 94 46 1 2 95 220 310 4.70e-20 87.4
MsaG038062 AT3G04450.3 54.412 68 28 1 9 76 234 298 1.36e-18 83.2
MsaG038062 AT1G79430.1 38.655 119 57 3 48 161 1 108 2.43e-18 82.4
MsaG038062 AT2G42660.1 61.905 63 21 1 15 77 52 111 9.66e-16 74.7
MsaG038062 AT2G06020.1 51.667 60 26 1 13 72 86 142 1.24e-15 75.1
MsaG038062 AT2G40260.1 60.345 58 20 1 15 72 84 138 2.79e-14 71.6
MsaG038062 AT3G04030.5 38.182 110 51 3 48 141 1 109 1.32e-13 69.3
MsaG038062 AT4G04605.1 51.351 74 32 2 15 88 16 85 2.12e-13 67.4
MsaG038062 AT5G42630.2 50.000 74 33 2 15 87 107 177 2.57e-13 67.4
MsaG038062 AT5G42630.1 48.148 81 38 2 15 94 107 184 3.44e-13 67.8
MsaG038062 AT4G17695.1 38.835 103 47 2 15 104 166 265 6.33e-12 64.7
MsaG038062 AT5G16560.1 46.575 73 34 2 15 85 221 290 1.33e-11 63.9
MsaG038062 AT2G38300.1 55.932 59 23 1 15 73 56 111 1.83e-11 63.2
MsaG038062 AT1G32240.2 53.448 58 24 1 15 72 215 269 2.34e-11 63.2
MsaG038062 AT2G02060.2 60.000 55 19 1 15 69 31 82 2.47e-11 62.4
MsaG038062 AT2G02060.1 60.000 55 19 1 15 69 31 82 2.47e-11 62.4
MsaG038062 AT1G14600.1 55.932 59 23 1 15 73 25 80 2.49e-11 62.4
MsaG038062 AT1G32240.1 53.448 58 24 1 15 72 215 269 2.54e-11 63.2
MsaG038062 AT4G37180.1 51.724 58 25 1 13 70 210 264 8.18e-11 61.6
MsaG038062 AT4G37180.2 51.724 58 25 1 13 70 217 271 8.58e-11 61.6

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTTACTTGTGGTGGATTC+TGG 0.193661 7:-70535879 None:intergenic
ACCAAACCTGGAACCTTTCT+TGG 0.232009 7:+70536846 MsaT038062.1:CDS
TACGATATAGAACAGAAATT+TGG 0.266828 7:-70536023 None:intergenic
GGTGCCAAGTTTCTTATTAT+AGG 0.282243 7:+70536904 MsaT038062.1:CDS
GGTGGATTCTGGAATCAATT+TGG 0.287077 7:-70535868 None:intergenic
GCACCTCTGCCTGCATTTGA+AGG 0.327265 7:-70536140 None:intergenic
TGGAGGCTGTAAATAGGCTT+GGG 0.332347 7:+70533851 MsaT038062.1:CDS
TCTTTGTGGAGGCTGTAAAT+AGG 0.333114 7:+70533845 MsaT038062.1:CDS
CGCTGTTCGACGTTCTTCAT+CGG 0.369962 7:-70536744 None:intergenic
TCCAAGAAAGGTTCCAGGTT+TGG 0.371544 7:-70536847 None:intergenic
TTGGATATGTTCAGAAATAC+AGG 0.386624 7:+70535841 MsaT038062.1:intron
ACACGTCAAAAGCCACTTGC+AGG 0.391889 7:+70534997 MsaT038062.1:CDS
GTGGAGGCTGTAAATAGGCT+TGG 0.397815 7:+70533850 MsaT038062.1:CDS
AAGAAGAGCTTGCAAGCCTT+TGG 0.398313 7:+70536661 MsaT038062.1:CDS
CTGACTCCAAGAAAGGTTCC+AGG 0.408019 7:-70536852 None:intergenic
GGAAAGTACCTAGACAGAAT+TGG 0.410201 7:+70536611 MsaT038062.1:CDS
AGCAACCTACCTTTCACCTT+TGG 0.415291 7:+70536883 MsaT038062.1:CDS
GAACCTTTCTTGGAGTCAGC+TGG 0.440714 7:+70536856 MsaT038062.1:CDS
ATTCCTTTCAGTATACCTCT+TGG 0.449845 7:-70534941 None:intergenic
CAGCCTCCACAAAGAGATCA+TGG 0.480797 7:-70533837 None:intergenic
GTTATTGAACTCTGAGGTGG+TGG 0.485508 7:-70536825 None:intergenic
CAGCCAGCTGACTCCAAGAA+AGG 0.496169 7:-70536859 None:intergenic
ACAACTCCATGATCTCTTTG+TGG 0.503279 7:+70533831 MsaT038062.1:CDS
ACTGAAAGGAATGAAGGCTA+TGG 0.503485 7:+70534952 MsaT038062.1:CDS
GAACAGTGCTCTTCAGCTAA+AGG 0.503631 7:+70536113 MsaT038062.1:intron
TACTTTGTCCAATTCTGTCT+AGG 0.510514 7:-70536619 None:intergenic
CACACAAAGAACGCTTAAGA+TGG 0.520735 7:+70533803 MsaT038062.1:CDS
CAGAGTTCAATAACCAAACC+TGG 0.524988 7:+70536834 MsaT038062.1:CDS
ATGGGTTCCACTCGCTCAGA+TGG 0.537642 7:+70533772 MsaT038062.1:CDS
AAAGGAACTCCTTCAAATGC+AGG 0.540215 7:+70536131 MsaT038062.1:CDS
AGGTATACTGAAAGGAATGA+AGG 0.543020 7:+70534946 MsaT038062.1:CDS
AATAAGAAACTTGGCACCAA+AGG 0.545841 7:-70536899 None:intergenic
TCGAACAGCGAAAATGCAAA+AGG 0.552298 7:+70536757 MsaT038062.1:CDS
AAAACCTATAATAAGAAACT+TGG 0.552729 7:-70536908 None:intergenic
AGAGCTTGCAAGCCTTTGGT+TGG 0.555317 7:+70536665 MsaT038062.1:CDS
CTGAAAGGAATGAAGGCTAT+GGG 0.555733 7:+70534953 MsaT038062.1:CDS
GAGGCTGTAAATAGGCTTGG+GGG 0.562654 7:+70533853 MsaT038062.1:CDS
TTCAGATTCCTCAGAAAGAG+AGG 0.566319 7:-70536705 None:intergenic
GATCCTAGTTCAAAACATGT+AGG 0.571888 7:-70536800 None:intergenic
GGAGGCTGTAAATAGGCTTG+GGG 0.579180 7:+70533852 MsaT038062.1:CDS
ACCTCTGCCCTCTCTTTCTG+AGG 0.584449 7:+70536697 MsaT038062.1:CDS
GGTGTTGCTTTACCAACCAA+AGG 0.587136 7:-70536677 None:intergenic
TCTTAAGCGTTCTTTGTGTG+TGG 0.591577 7:-70533800 None:intergenic
AACTTGGCACCAAAGGTGAA+AGG 0.593004 7:-70536892 None:intergenic
ACTCCTTCAAATGCAGGCAG+AGG 0.602704 7:+70536137 MsaT038062.1:CDS
TTGGTTATTGAACTCTGAGG+TGG 0.604572 7:-70536828 None:intergenic
TCCTCAGAAAGAGAGGGCAG+AGG 0.605704 7:-70536698 None:intergenic
GATGATCTATGAAGGAGGAA+AGG 0.610887 7:+70535971 MsaT038062.1:intron
GGCAGAGCCATCTGAGCGAG+TGG 0.612768 7:-70533779 None:intergenic
ACGCATGAGTGATAAAGTTG+AGG 0.616870 7:+70536167 MsaT038062.1:CDS
TGGCACCAAAGGTGAAAGGT+AGG 0.623055 7:-70536888 None:intergenic
ACACCAAGAGGTATACTGAA+AGG 0.630195 7:+70534938 MsaT038062.1:CDS
GGTTTGGTTATTGAACTCTG+AGG 0.639747 7:-70536831 None:intergenic
ACTCCATGATCTCTTTGTGG+AGG 0.654213 7:+70533834 MsaT038062.1:CDS
TTGTCAGGGGCAACACCAAG+AGG 0.658933 7:+70534926 MsaT038062.1:intron
TCAGATTCCTCAGAAAGAGA+GGG 0.661556 7:-70536704 None:intergenic
ATGGGTGAAGAACCTGCAAG+TGG 0.669007 7:-70535009 None:intergenic
AGGCTGTAAATAGGCTTGGG+GGG 0.689265 7:+70533854 MsaT038062.1:CDS
AAGGTGAAAATTGAAGCACA+AGG 0.725184 7:+70536590 MsaT038062.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 70533772 70536925 70533772 ID=MsaG038062
Chr7 mRNA 70533772 70536925 70533772 ID=MsaT038062.1;Parent=MsaG038062
Chr7 exon 70533772 70533883 70533772 ID=MsaT038062.1.exon1;Parent=MsaT038062.1
Chr7 CDS 70533772 70533883 70533772 ID=cds.MsaT038062.1;Parent=MsaT038062.1
Chr7 exon 70534933 70535018 70534933 ID=MsaT038062.1.exon2;Parent=MsaT038062.1
Chr7 CDS 70534933 70535018 70534933 ID=cds.MsaT038062.1;Parent=MsaT038062.1
Chr7 exon 70535855 70535894 70535855 ID=MsaT038062.1.exon3;Parent=MsaT038062.1
Chr7 CDS 70535855 70535894 70535855 ID=cds.MsaT038062.1;Parent=MsaT038062.1
Chr7 exon 70535985 70536042 70535985 ID=MsaT038062.1.exon4;Parent=MsaT038062.1
Chr7 CDS 70535985 70536042 70535985 ID=cds.MsaT038062.1;Parent=MsaT038062.1
Chr7 exon 70536119 70536188 70536119 ID=MsaT038062.1.exon5;Parent=MsaT038062.1
Chr7 CDS 70536119 70536188 70536119 ID=cds.MsaT038062.1;Parent=MsaT038062.1
Chr7 exon 70536575 70536925 70536575 ID=MsaT038062.1.exon6;Parent=MsaT038062.1
Chr7 CDS 70536575 70536925 70536575 ID=cds.MsaT038062.1;Parent=MsaT038062.1
Gene Sequence

>MsaT038062.1

ATGGGTTCCACTCGCTCAGATGGCTCTGCCACACACAAAGAACGCTTAAGATGGACACAACAACTCCATGATCTCTTTGTGGAGGCTGTAAATAGGCTTGGGGGGCCAGATAGGGCAACACCAAGAGGTATACTGAAAGGAATGAAGGCTATGGGAGTTTCAGAATTGAAAATATTACACGTCAAAAGCCACTTGCAGAAATACAGGATTTCCAAATTGATTCCAGAATCCACCACAAGAGGAAAGGTTGAGAAGAGAAGCATATCAGATATACTGCCAAATTTCTGTTCTATATCTGCTCTTCAGCTAAAGGAACTCCTTCAAATGCAGGCAGAGGTGCAAAAACGCATGAGTGATAAAGTTGAGGTTCAAAAAAGCTTGAAGGTGAAAATTGAAGCACAAGGAAAGTACCTAGACAGAATTGGACAAAGTAATCAAATCAAAACAATTACAAGAAGAGCTTGCAAGCCTTTGGTTGGTAAAGCAACACCTCTGCCCTCTCTTTCTGAGGAATCTGAATCCTTTAAAACACAATCCGATGAAGAACGTCGAACAGCGAAAATGCAAAAGGTCACAGATGAGAATGTTTTTCCTACATGTTTTGAACTAGGATCATCCACCACCTCAGAGTTCAATAACCAAACCTGGAACCTTTCTTGGAGTCAGCTGGCTGAAGCAACCTACCTTTCACCTTTGGTGCCAAGTTTCTTATTATAG

Protein sequence

>MsaT038062.1

MGSTRSDGSATHKERLRWTQQLHDLFVEAVNRLGGPDRATPRGILKGMKAMGVSELKILHVKSHLQKYRISKLIPESTTRGKVEKRSISDILPNFCSISALQLKELLQMQAEVQKRMSDKVEVQKSLKVKIEAQGKYLDRIGQSNQIKTITRRACKPLVGKATPLPSLSEESESFKTQSDEERRTAKMQKVTDENVFPTCFELGSSTTSEFNNQTWNLSWSQLAEATYLSPLVPSFLL*