AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038115

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038115 MtrunA17_Chr7g0243041 91.195 159 8 1 1 159 1 153 9.17e-90 258
MsaG038115 MtrunA17_Chr3g0134001 61.765 68 26 0 18 85 163 230 1.01e-24 96.3
MsaG038115 MtrunA17_Chr8g0384141 62.121 66 25 0 22 87 110 175 2.39e-23 92.4
MsaG038115 MtrunA17_Chr5g0435021 66.038 53 18 0 33 85 225 277 2.61e-18 79.7
MsaG038115 MtrunA17_Chr8g0375121 59.459 74 30 0 18 91 124 197 4.35e-18 78.2
MsaG038115 MtrunA17_Chr5g0402161 59.459 74 30 0 18 91 129 202 4.62e-18 78.2
MsaG038115 MtrunA17_Chr1g0152651 51.515 66 28 1 22 87 274 335 1.47e-17 78.2
MsaG038115 MtrunA17_Chr5g0393721 58.333 60 25 0 28 87 226 285 2.33e-17 77.4
MsaG038115 MtrunA17_Chr1g0207301 40.206 97 47 2 20 108 285 378 3.71e-17 77.0
MsaG038115 MtrunA17_Chr1g0207311 52.308 65 30 1 23 87 194 257 1.82e-16 74.3
MsaG038115 MtrunA17_Chr8g0341421 56.757 74 32 0 23 96 325 398 3.49e-16 74.7
MsaG038115 MtrunA17_Chr2g0330641 50.000 78 39 0 22 99 286 363 1.08e-15 73.2
MsaG038115 MtrunA17_Chr3g0134741 50.769 65 28 1 23 87 230 290 1.19e-15 72.4
MsaG038115 MtrunA17_Chr4g0074161 37.931 87 50 1 3 85 179 265 3.75e-13 65.9
MsaG038115 MtrunA17_Chr1g0179061 36.986 73 46 0 22 94 166 238 3.03e-12 62.4
MsaG038115 MtrunA17_Chr1g0190731 37.333 75 47 0 11 85 178 252 6.78e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038115 AT5G01305.1 66.265 83 28 0 15 97 26 108 2.98e-35 121
MsaG038115 AT3G21330.1 47.727 88 46 0 22 109 265 352 2.78e-22 91.7
MsaG038115 AT4G00120.1 53.030 66 31 0 22 87 108 173 3.80e-20 82.8
MsaG038115 AT5G43175.1 39.496 119 56 3 2 105 102 219 4.52e-20 83.2
MsaG038115 AT1G27740.1 43.103 116 52 4 12 124 150 254 9.30e-19 80.5
MsaG038115 AT4G33880.1 53.030 66 27 1 22 87 266 327 7.37e-18 79.3
MsaG038115 AT5G67060.1 63.235 68 25 0 18 85 114 181 7.87e-18 77.8
MsaG038115 AT5G67060.2 63.235 68 25 0 18 85 102 169 8.08e-18 77.4
MsaG038115 AT3G50330.1 63.235 68 25 0 18 85 111 178 1.42e-17 77.0
MsaG038115 AT5G09750.1 66.667 66 22 0 22 87 115 180 3.72e-17 75.5
MsaG038115 AT1G66470.1 48.235 85 42 2 3 85 170 254 5.19e-17 76.3
MsaG038115 AT2G14760.1 43.023 86 37 2 22 99 237 318 1.15e-16 75.9
MsaG038115 AT5G37800.1 60.784 51 20 0 35 85 210 260 2.43e-16 74.7
MsaG038115 AT4G30980.1 44.000 75 38 1 11 85 119 189 4.33e-14 68.6
MsaG038115 AT4G30980.2 44.000 75 38 1 11 85 167 237 5.63e-14 68.6
MsaG038115 AT2G24260.2 52.727 55 26 0 33 87 42 96 9.93e-14 67.0
MsaG038115 AT2G14760.3 37.374 99 37 3 22 99 237 331 2.40e-13 66.6
MsaG038115 AT2G24260.1 54.717 53 24 0 33 85 145 197 2.53e-13 66.6
MsaG038115 AT5G58010.1 43.478 69 35 1 17 85 94 158 2.90e-12 63.2

Find 23 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACTTTCTCTTATTCTTA+TGG 0.169939 7:-71593028 MsaT038115.1:CDS
ATTAACTTTGTTGATGATAT+TGG 0.283073 7:-71592764 MsaT038115.1:CDS
TCTCTAGCTGCAACACTTTG+TGG 0.303892 7:+71592932 None:intergenic
ACACTTTCTCTTATTCTTAT+GGG 0.318721 7:-71593027 MsaT038115.1:CDS
TAGACTCAAATGAACCTAAA+AGG 0.329354 7:+71592672 None:intergenic
GATCCCTGGTGGAAGTAAAT+TGG 0.340425 7:-71592864 MsaT038115.1:CDS
AAGTTTCACTGAACTTCCTT+TGG 0.367586 7:+71592964 None:intergenic
AGTTTCTTAAGAAACAGATT+TGG 0.417653 7:-71592802 MsaT038115.1:CDS
ACCTAAAAGGTTGTGCTGAA+TGG 0.469930 7:+71592685 None:intergenic
TTTCATAGTGGCATCAAAGA+TGG 0.476710 7:+71592586 None:intergenic
ATTGGACACTGTTTCAATGT+TGG 0.489547 7:-71592846 MsaT038115.1:CDS
GAAGACATAGAATCAGTGAT+AGG 0.495589 7:-71592907 MsaT038115.1:CDS
GGACACTGTTTCAATGTTGG+AGG 0.517367 7:-71592843 MsaT038115.1:CDS
TGGGTCATGTTCAAAGAGAA+AGG 0.518124 7:-71593008 MsaT038115.1:CDS
TGAAAATAGAACTGCTCTAG+TGG 0.553561 7:+71592629 None:intergenic
TTTCTTAGCTAGCACATGGA+TGG 0.556000 7:-71593052 None:intergenic
GGCTAATTAATATTTCATAG+TGG 0.574341 7:+71592574 None:intergenic
GTGTCCAATTTACTTCCACC+AGG 0.595621 7:+71592860 None:intergenic
TCATTTGAAGAATAATCTTG+AGG 0.620010 7:+71592722 None:intergenic
ATTCTTCAGAGTATGATCCC+TGG 0.631016 7:-71592878 MsaT038115.1:CDS
CTTCAGAGTATGATCCCTGG+TGG 0.671280 7:-71592875 MsaT038115.1:CDS
AACTGCTCTAGTGGTAACTG+TGG 0.680734 7:+71592638 None:intergenic
TGTCCAATTTACTTCCACCA+GGG 0.712775 7:+71592861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 71592580 71593059 71592580 ID=MsaG038115
Chr7 mRNA 71592580 71593059 71592580 ID=MsaT038115.1;Parent=MsaG038115
Chr7 exon 71592580 71593059 71592580 ID=MsaT038115.1.exon1;Parent=MsaT038115.1
Chr7 CDS 71592580 71593059 71592580 ID=cds.MsaT038115.1;Parent=MsaT038115.1
Gene Sequence

>MsaT038115.1

ATGGATGGTAACACTTTCTCTTATTCTTATGGGTCATGTTCAAAGAGAAAGGAGAAAAAAGGTGTGAAAAAATCCAAAGGAAGTTCAGTGAAACTTTCAACTGATCCACAAAGTGTTGCAGCTAGAGAAAGAAGACATAGAATCAGTGATAGGTTCAAGATTCTTCAGAGTATGATCCCTGGTGGAAGTAAATTGGACACTGTTTCAATGTTGGAGGAAGCTATTCATTATGTTAAGTTTCTTAAGAAACAGATTTGGCTTCATGAAACATTGATTAACTTTGTTGATGATATTGGTGAGTCACACATGTTGCTTCCTCAAGATTATTCTTCAAATGATAAAAATATTCATTCCATTCAGCACAACCTTTTAGGTTCATTTGAGTCTATGCAGAATTTGCCACAGTTACCACTAGAGCAGTTCTATTTTCAAGGAGATGAAGATATGAAAACCATCTTTGATGCCACTATGAAATATTAA
Protein sequence

>MsaT038115.1

MDGNTFSYSYGSCSKRKEKKGVKKSKGSSVKLSTDPQSVAARERRHRISDRFKILQSMIPGGSKLDTVSMLEEAIHYVKFLKKQIWLHETLINFVDDIGESHMLLPQDYSSNDKNIHSIQHNLLGSFESMQNLPQLPLEQFYFQGDEDMKTIFDATMKY*