AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038120 MtrunA17_Chr7g0243131 96.603 677 13 1 256 922 1 677 0.0 1345
MsaG038120 MtrunA17_Chr4g0005681 70.860 954 228 11 1 922 1 936 0.0 1332
MsaG038120 MtrunA17_Chr3g0105811 58.735 933 343 11 9 921 1 911 0.0 1048
MsaG038120 MtrunA17_Chr1g0185181 50.176 851 378 7 90 922 51 873 0.0 776
MsaG038120 MtrunA17_Chr7g0243111 95.385 195 8 1 1 195 1 194 1.22e-128 385
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038120 AT3G51770.2 68.553 954 258 8 1 922 1 944 0.0 1310
MsaG038120 AT3G51770.1 68.605 946 255 8 9 922 1 936 0.0 1298
MsaG038120 AT5G58550.1 53.022 943 373 10 9 922 1 902 0.0 956
MsaG038120 AT5G58550.1 27.149 221 149 3 715 924 356 575 2.19e-13 75.1
MsaG038120 AT4G02680.1 49.527 846 393 6 90 922 51 875 0.0 763

Find 221 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAGGCTAGTTTGGTTTC+AGG 0.109106 7:+71677194 MsaT038120.1:CDS
TTGTAGGCACTCAATAATTT+AGG 0.154747 7:+71680489 MsaT038120.1:intron
AGCTTCCAGTGTGGCTGTTT+TGG 0.165102 7:-71676841 None:intergenic
CCATAGAACATCATATAATT+TGG 0.168779 7:-71678427 None:intergenic
CCAAATTATATGATGTTCTA+TGG 0.202581 7:+71678427 MsaT038120.1:CDS
TCTGAAATCAACAAAATAAT+TGG 0.204338 7:+71678298 MsaT038120.1:CDS
GCTAGTGGCCGCTTGCTTAC+AGG 0.226171 7:+71677706 MsaT038120.1:CDS
AGATGTAACAGTTGCAATTC+TGG 0.227845 7:-71682018 None:intergenic
CGTTGATGATATTGGTTCTT+TGG 0.244913 7:+71678549 MsaT038120.1:CDS
TCTCTTTGTATCAGTGTTAA+TGG 0.257847 7:+71681939 MsaT038120.1:intron
GCATTATTGTTGATTGAGTA+TGG 0.258936 7:+71677662 MsaT038120.1:CDS
AAAACAGCCCGGTGTTTATA+TGG 0.268161 7:-71678241 None:intergenic
ATCTGCTTGTGATGCTCATT+TGG 0.276733 7:+71677613 MsaT038120.1:CDS
ACCTGTTGGTTGGATGTATC+AGG 0.286915 7:+71678138 MsaT038120.1:CDS
TTTAGTTCGACCGGAAGTTT+TGG 0.287133 7:-71676808 None:intergenic
CAAACATCACAACACCTAAT+TGG 0.292783 7:-71677961 None:intergenic
CCATACAACATTGTCATAAA+AGG 0.296170 7:-71677416 None:intergenic
CCCTCGCCGGCGTGCGTCGC+CGG 0.297324 7:+71676946 MsaT038120.1:CDS
CAACTTGCTTATCACCAATT+AGG 0.297340 7:+71677947 MsaT038120.1:CDS
TGATGCTCATTTGGCTGCTT+TGG 0.298085 7:+71677622 MsaT038120.1:CDS
CGGAGAATCGGCGGCGGCGG+CGG 0.298093 7:-71676718 None:intergenic
TGTGTGACCACGCTTATATT+TGG 0.311310 7:-71678071 None:intergenic
TGGTTCCACTTCTGAGCAAC+AGG 0.313807 7:-71678487 None:intergenic
CATCTCATCATATGCCGCTT+TGG 0.316982 7:-71680634 None:intergenic
ATCTCATCATATGCCGCTTT+GGG 0.317455 7:-71680633 None:intergenic
AATACAAAGATGCACAAAAT+TGG 0.320811 7:+71677993 MsaT038120.1:CDS
GTATAGGTGATAGTGAAATT+AGG 0.322217 7:+71677360 MsaT038120.1:CDS
AAACTAAGATCGTTCTTTGC+AGG 0.329287 7:-71680730 None:intergenic
CAATTCTGGCTTAAAGTTGA+TGG 0.331764 7:-71682004 None:intergenic
CAGGTTTAAGATCAGAAATA+AGG 0.339519 7:-71678120 None:intergenic
ACCAAGAGGTTGAGTCAGTT+TGG 0.340955 7:+71677530 MsaT038120.1:CDS
TGCTGAAGAAGAATGATTTC+TGG 0.341115 7:-71680132 None:intergenic
AAAGAGTGTGATTGATCTCT+TGG 0.347427 7:+71680316 MsaT038120.1:CDS
GGACATATCCGTACAGATAT+AGG 0.348680 7:+71680776 MsaT038120.1:CDS
TGTAGAGGGTAGAGATAGAT+TGG 0.355541 7:+71677790 MsaT038120.1:CDS
CTAAGGCTAGTTTGGTTTCA+GGG 0.356896 7:+71677195 MsaT038120.1:CDS
TCAGGGTGTGATCATGAATC+AGG 0.357911 7:+71677212 MsaT038120.1:CDS
TGAATGTCCTAAGGCTAGTT+TGG 0.358860 7:+71677187 MsaT038120.1:CDS
GACATATCCGTACAGATATA+GGG 0.382877 7:+71680777 MsaT038120.1:CDS
TGCAAAGAACGATCTTAGTT+TGG 0.384325 7:+71680732 MsaT038120.1:CDS
TTCTTGGAGGAGAATAAGAT+TGG 0.384988 7:+71678265 MsaT038120.1:CDS
AAGCAATTGCAGAGCTTTCA+AGG 0.386541 7:+71681980 MsaT038120.1:CDS
AGCTGACCCGTGTCTTGATT+CGG 0.391453 7:+71680286 MsaT038120.1:CDS
TGGTCTTCCGTTGATGATAT+TGG 0.396259 7:+71678541 MsaT038120.1:CDS
TTGTTTGAAGCCGTTTGATT+TGG 0.402048 7:+71676917 MsaT038120.1:CDS
TGTAGGCACTCAATAATTTA+GGG 0.405280 7:+71680490 MsaT038120.1:intron
CCAAGAGGTTGAGTCAGTTT+GGG 0.407653 7:+71677531 MsaT038120.1:CDS
AACTTCCGGTCGAACTAAAC+CGG 0.409139 7:+71676812 MsaT038120.1:CDS
GGCTCCTTCATAATCCTTCA+TGG 0.410046 7:-71678371 None:intergenic
CAAGCATACACGAGCACATC+AGG 0.413519 7:+71680578 MsaT038120.1:CDS
CTGTACGGATATGTCCTTAG+AGG 0.414002 7:-71680769 None:intergenic
GGCAATCTTTACTTCTGTTA+CGG 0.416098 7:+71678626 MsaT038120.1:CDS
TTTCTGATAGTAAATTGTTC+CGG 0.416330 7:+71677030 MsaT038120.1:CDS
TCCTGATACATCCAACCAAC+AGG 0.422721 7:-71678139 None:intergenic
TTGGTTCGAGGCTGCTGTTA+AGG 0.424110 7:+71678012 MsaT038120.1:CDS
ATTTCTGATCTTAAACCTGT+TGG 0.424740 7:+71678124 MsaT038120.1:CDS
CAACACAGTTGGATCCTCTA+AGG 0.425094 7:+71680755 MsaT038120.1:CDS
TATATGGAAAAGAAAGAGTT+GGG 0.429439 7:-71678225 None:intergenic
TTGGTTCTTGATTGCCATGA+AGG 0.431469 7:+71678357 MsaT038120.1:CDS
TCCTTGTGGCCTTCCTTCGT+CGG 0.433566 7:+71676869 MsaT038120.1:CDS
GTTAGTGTTGATAATGTTGA+TGG 0.438872 7:+71677272 MsaT038120.1:CDS
AATGTCCTTCGGATGGTCTT+CGG 0.439137 7:+71680351 MsaT038120.1:CDS
CTAGACCGCTCCAAAACTTC+CGG 0.441185 7:+71676798 MsaT038120.1:CDS
TGAAGATTGTGTTGGCTGCT+TGG 0.442390 7:+71677096 MsaT038120.1:CDS
AGTGTTAATGGACGATCATA+AGG 0.446891 7:+71681951 MsaT038120.1:intron
TTGGGAATGATCTTGTGCTT+GGG 0.447922 7:+71677549 MsaT038120.1:CDS
AAGGAGCCCTTAGGGATGTC+AGG 0.448282 7:+71678386 MsaT038120.1:CDS
CCGGCGACGCACGCCGGCGA+GGG 0.449553 7:-71676946 None:intergenic
AGCCATGCGTAGTTTGCGTC+TGG 0.451196 7:+71680109 MsaT038120.1:CDS
TTTGGGAATGATCTTGTGCT+TGG 0.451623 7:+71677548 MsaT038120.1:CDS
TTGCAGGTTAAATTCTCAAA+AGG 0.451688 7:+71680085 MsaT038120.1:intron
GCTCTCAACTCAAGGCAGTC+AGG 0.452272 7:-71678334 None:intergenic
AATTGTTCCGGCGGAGTTTG+CGG 0.455194 7:+71677042 MsaT038120.1:CDS
AGGATTATGAAGGAGCCCTT+AGG 0.458477 7:+71678377 MsaT038120.1:CDS
TTCTTCAGTGGGTGTTGCAA+GGG 0.462733 7:+71678048 MsaT038120.1:CDS
CGCCGGATCGAGGGTGAGGG+TGG 0.464077 7:+71676963 MsaT038120.1:CDS
GCTTGTTGGTTCTTCTGCAA+TGG 0.465046 7:+71677145 MsaT038120.1:CDS
GCGTCGCCGGATCGAGGGTG+AGG 0.467299 7:+71676959 MsaT038120.1:CDS
ACTGCCTTGAGTTGAGAGCT+TGG 0.469236 7:+71678338 MsaT038120.1:CDS
CCGCCGATGACGGCGTTGAT+CGG 0.472391 7:-71676738 None:intergenic
AAAGGGCCGCTTCACAGTCT+TGG 0.472906 7:-71682086 None:intergenic
TCCCACCCTCACCCTCGATC+CGG 0.475722 7:-71676965 None:intergenic
ATTCTTCAGTGGGTGTTGCA+AGG 0.476193 7:+71678047 MsaT038120.1:CDS
GATCGGAGAATCGGCGGCGG+CGG 0.476394 7:-71676721 None:intergenic
TCATTTGGCTGCTTTGGTGT+TGG 0.479480 7:+71677628 MsaT038120.1:CDS
AGGTTTAAGATCAGAAATAA+GGG 0.480132 7:-71678119 None:intergenic
AAAGGTCTTTGTATTGCATC+GGG 0.480166 7:+71678161 MsaT038120.1:CDS
TCTCCGATCAACGCCGTCAT+CGG 0.480841 7:+71676735 MsaT038120.1:CDS
CGGCGTGCGTCGCCGGATCG+AGG 0.481001 7:+71676953 MsaT038120.1:CDS
TTGTGTTGGCTGCTTGGTTG+AGG 0.481404 7:+71677102 MsaT038120.1:CDS
GGATTATGAAGGAGCCCTTA+GGG 0.483017 7:+71678378 MsaT038120.1:CDS
TCCGGCGACGCACGCCGGCG+AGG 0.483042 7:-71676947 None:intergenic
AGAAGAGAAGATGAGCTTGT+TGG 0.485086 7:+71677131 MsaT038120.1:CDS
GGATGGTCTTCGGAAGGGAC+AGG 0.489334 7:+71680361 MsaT038120.1:CDS
ATCAACGCCGTCATCGGCGG+CGG 0.491426 7:+71676741 MsaT038120.1:CDS
GATGACAAAGCTGATAGAAA+AGG 0.492033 7:+71680653 MsaT038120.1:CDS
ATGGTGGATTTGTTGAGTCT+AGG 0.494400 7:+71677435 MsaT038120.1:CDS
TGAGGTGATGAGGGCGGTTG+AGG 0.499232 7:+71677496 MsaT038120.1:CDS
TCTTAGTTTGGCAACACAGT+TGG 0.499800 7:+71680744 MsaT038120.1:CDS
GACGGCGTTGATCGGAGAAT+CGG 0.500768 7:-71676730 None:intergenic
CCCAAACTGACTCAACCTCT+TGG 0.501232 7:-71677531 None:intergenic
TTCGCCCTGTTGCTCAGAAG+TGG 0.502085 7:+71678482 MsaT038120.1:CDS
CTCGATCCGGCGACGCACGC+CGG 0.504675 7:-71676952 None:intergenic
GGAAGCTCTTAAATGTCCTT+CGG 0.504692 7:+71680340 MsaT038120.1:CDS
AGGGTTTGAACCAAATCAAA+CGG 0.505034 7:-71676927 None:intergenic
CCTGAAACCAAACTAGCCTT+AGG 0.506291 7:-71677194 None:intergenic
GGCGTGCGTCGCCGGATCGA+GGG 0.508211 7:+71676954 MsaT038120.1:CDS
GAAAGGTCTTTGTATTGCAT+CGG 0.511296 7:+71678160 MsaT038120.1:CDS
TGTTGCTCAGAAGTGGAACC+AGG 0.514083 7:+71678489 MsaT038120.1:CDS
GGAATCGCCGCCGCCGATGA+CGG 0.515016 7:-71676748 None:intergenic
ATTGTTGATTGAGTATGGAT+TGG 0.515047 7:+71677667 MsaT038120.1:CDS
GGACATATTTATTCTTCAGT+GGG 0.515693 7:+71678037 MsaT038120.1:CDS
CTCAAACGTTGCATCGAGCT+AGG 0.516884 7:-71677746 None:intergenic
CCGTACAGATATAGGGCGGC+AGG 0.518690 7:+71680784 MsaT038120.1:CDS
TCCAACCGGTTTAGTTCGAC+CGG 0.518891 7:-71676817 None:intergenic
TGGAGCGGTCTAGTAAATGA+TGG 0.518963 7:-71676788 None:intergenic
GTGATGCTCTTAGAGAGGTT+AGG 0.522199 7:+71677896 MsaT038120.1:CDS
GTTGAATCACAGGAGGGAAA+AGG 0.523398 7:+71682335 MsaT038120.1:intron
GTTGATTGAGTATGGATTGG+AGG 0.524701 7:+71677670 MsaT038120.1:CDS
CATGAAAGACTTGTCTATGA+AGG 0.526341 7:+71680155 MsaT038120.1:CDS
CAAATTATATGATGTTCTAT+GGG 0.529449 7:+71678428 MsaT038120.1:CDS
CTGATCTTAAACCTGTTGGT+TGG 0.530968 7:+71678128 MsaT038120.1:CDS
CTAGAAGCTCCGACGAAGGA+AGG 0.534045 7:-71676878 None:intergenic
GCGCCGACCGCAAACTCCGC+CGG 0.534125 7:-71677049 None:intergenic
AGGACATATTTATTCTTCAG+TGG 0.535820 7:+71678036 MsaT038120.1:CDS
CTGGAAGCTCTTCTTCCTTG+TGG 0.535909 7:+71676855 MsaT038120.1:CDS
AACTAAGATCGTTCTTTGCA+GGG 0.536067 7:-71680729 None:intergenic
AATGGATTGTTGTGGAAGAA+AGG 0.538901 7:+71677163 MsaT038120.1:CDS
AGTGGAACCAGGCTGATTGT+TGG 0.540888 7:+71678500 MsaT038120.1:CDS
AAATTGCCCTGACATCCCTA+AGG 0.545348 7:-71678393 None:intergenic
TTATATGGAAAAGAAAGAGT+TGG 0.545500 7:-71678226 None:intergenic
CCGATCAACGCCGTCATCGG+CGG 0.546042 7:+71676738 MsaT038120.1:CDS
CGTCGCCGGATCGAGGGTGA+GGG 0.548713 7:+71676960 MsaT038120.1:CDS
ATTGCCATGAAGGATTATGA+AGG 0.549623 7:+71678367 MsaT038120.1:CDS
GAATATGAAAGGCGATCGCT+TGG 0.549978 7:+71678450 MsaT038120.1:CDS
ATGTTCTATGGGAATATGAA+AGG 0.551432 7:+71678439 MsaT038120.1:CDS
GTTCCGGCGGAGTTTGCGGT+CGG 0.559799 7:+71677046 MsaT038120.1:CDS
TTTGAGTCAGGTTGCGATGG+AGG 0.560395 7:+71677850 MsaT038120.1:CDS
AGGAGTCGATTTCTATTCAA+AGG 0.560635 7:+71680228 MsaT038120.1:CDS
AAATCAAACGGCTTCAAACA+AGG 0.560982 7:-71676915 None:intergenic
TCGTGCTTCGGATCGAGGTG+AGG 0.563792 7:-71676647 None:intergenic
GAGTGTGATTGATCTCTTGG+AGG 0.564967 7:+71680319 MsaT038120.1:CDS
TCTAACACCTCAGCATGGCT+AGG 0.565229 7:-71682117 None:intergenic
ATGGCTAGGATCAAGACAAA+GGG 0.566751 7:-71682103 None:intergenic
GAGATAGATTGGCAGCGGTA+GGG 0.567075 7:+71677801 MsaT038120.1:CDS
TCCGGTCGAACTAAACCGGT+TGG 0.568000 7:+71676816 MsaT038120.1:CDS
TGGATTTGTTGAGTCTAGGA+GGG 0.568303 7:+71677439 MsaT038120.1:CDS
GGTTCTAGAAGCTCCGACGA+AGG 0.568479 7:-71676882 None:intergenic
TGTGCATTTGAAGATTGTGT+TGG 0.569827 7:+71677088 MsaT038120.1:CDS
AAAGACTTGTCTATGAAGGA+TGG 0.571019 7:+71680159 MsaT038120.1:CDS
TTCAAACAAGGCTCAATCGA+AGG 0.571307 7:-71676903 None:intergenic
GTGGATTTGTTGAGTCTAGG+AGG 0.573651 7:+71677438 MsaT038120.1:CDS
TGAGTATGGATTGGAGGAGA+TGG 0.573672 7:+71677676 MsaT038120.1:CDS
AGAACCAAGCTCTCAACTCA+AGG 0.576516 7:-71678342 None:intergenic
GGGTAGAGATAGATTGGCAG+CGG 0.579682 7:+71677796 MsaT038120.1:CDS
GGCCAGACGCAAACTACGCA+TGG 0.580332 7:-71680111 None:intergenic
GTCTTTGTATTGCATCGGGA+AGG 0.580398 7:+71678165 MsaT038120.1:CDS
TTCGAGGCTGCTGTTAAGGA+AGG 0.580450 7:+71678016 MsaT038120.1:CDS
GATCCGAAGCACGAAAATCA+CGG 0.580778 7:+71676656 MsaT038120.1:CDS
AGATGCACAAAATTGGTTCG+AGG 0.581179 7:+71678000 MsaT038120.1:CDS
TTAGTGTTGATAATGTTGAT+GGG 0.581210 7:+71677273 MsaT038120.1:CDS
TCTTCTGCAATGGATTGTTG+TGG 0.581613 7:+71677155 MsaT038120.1:CDS
TCCTTCGGATGGTCTTCGGA+AGG 0.581679 7:+71680355 MsaT038120.1:CDS
TTTATGACAATGTTGTATGG+TGG 0.584334 7:+71677419 MsaT038120.1:CDS
TGGACCCAAAACAGCCACAC+TGG 0.586338 7:+71676836 MsaT038120.1:CDS
AAGAAAGGTTGAATGTCCTA+AGG 0.586454 7:+71677178 MsaT038120.1:CDS
AGAAGCAATAGCAAAGGCTG+AGG 0.587077 7:+71680208 MsaT038120.1:CDS
TTAGGAGAATGTGCGATGAA+TGG 0.588635 7:+71677914 MsaT038120.1:CDS
GATTTGGTTCAAACCCTCGC+CGG 0.591682 7:+71676933 MsaT038120.1:CDS
GGCGTTGATCGGAGAATCGG+CGG 0.592552 7:-71676727 None:intergenic
ACTTCTGAGCAACAGGGCGA+AGG 0.593862 7:-71678480 None:intergenic
TCAAACGTTGCATCGAGCTA+GGG 0.597450 7:-71677745 None:intergenic
ATTGCATCCAACAATCAGCC+TGG 0.597846 7:-71678507 None:intergenic
AACTTCTTATGAAGAAGATG+AGG 0.604767 7:+71677310 MsaT038120.1:CDS
CCTGCCGCCCTATATCTGTA+CGG 0.604893 7:-71680784 None:intergenic
CAGATGTTAGAAAATAACCC+GGG 0.605466 7:+71678583 MsaT038120.1:CDS
CCAGATGTTAGAAAATAACC+CGG 0.605861 7:+71678582 MsaT038120.1:CDS
GCCGGATCGAGGGTGAGGGT+GGG 0.606510 7:+71676964 MsaT038120.1:CDS
AGCAATTGCAGAGCTTTCAA+GGG 0.606547 7:+71681981 MsaT038120.1:CDS
TACACGAGCACATCAGGGGT+TGG 0.606733 7:+71680584 MsaT038120.1:CDS
GAGAATGTGCGATGAATGGT+TGG 0.608510 7:+71677918 MsaT038120.1:CDS
TTGGTTGGATGTATCAGGAA+AGG 0.609801 7:+71678143 MsaT038120.1:CDS
AGAGATAGATTGGCAGCGGT+AGG 0.613127 7:+71677800 MsaT038120.1:CDS
TCTTGATCCTAGCCATGCTG+AGG 0.613827 7:+71682110 MsaT038120.1:CDS
CATGGCTAGGATCAAGACAA+AGG 0.613870 7:-71682104 None:intergenic
TACTGTCCAAGACTGTGAAG+CGG 0.615288 7:+71682080 MsaT038120.1:CDS
CAAAGAACCAATATCATCAA+CGG 0.617972 7:-71678548 None:intergenic
AGGAGCCCTTAGGGATGTCA+GGG 0.619291 7:+71678387 MsaT038120.1:CDS
GCTCTTAAATGTCCTTCGGA+TGG 0.621082 7:+71680344 MsaT038120.1:CDS
TCCGACGAAGGAAGGCCACA+AGG 0.625348 7:-71676870 None:intergenic
ACACGGGTCAGCTAATGCAT+AGG 0.626512 7:-71680276 None:intergenic
CCTTCGGATGGTCTTCGGAA+GGG 0.626775 7:+71680356 MsaT038120.1:CDS
CAGAAACACCTGTAAGCAAG+CGG 0.626901 7:-71677714 None:intergenic
AAGCATACACGAGCACATCA+GGG 0.626921 7:+71680579 MsaT038120.1:CDS
CTGATAGTAAATTGTTCCGG+CGG 0.627378 7:+71677033 MsaT038120.1:CDS
GGCGGAGTTTGCGGTCGGCG+CGG 0.630626 7:+71677051 MsaT038120.1:CDS
AATTGCCCTGACATCCCTAA+GGG 0.632546 7:-71678392 None:intergenic
GGAGATGGCGTATTTGCTAG+TGG 0.632550 7:+71677691 MsaT038120.1:CDS
TCTTATGAAGAAGATGAGGA+TGG 0.642156 7:+71677314 MsaT038120.1:CDS
GCTTGTAGCAGTCAGCAGCA+AGG 0.642893 7:-71680542 None:intergenic
AAATTTCTAACACCTCAGCA+TGG 0.643658 7:-71682122 None:intergenic
TAGTGTTGATAATGTTGATG+GGG 0.647542 7:+71677274 MsaT038120.1:CDS
TCGTGAAGAAGCAATAGCAA+AGG 0.650394 7:+71680202 MsaT038120.1:CDS
CGAAGCACGAAAATCACGGA+TGG 0.654802 7:+71676660 MsaT038120.1:CDS
GGTTCCACTTCTGAGCAACA+GGG 0.656342 7:-71678486 None:intergenic
GTTGATCGGAGAATCGGCGG+CGG 0.659465 7:-71676724 None:intergenic
CGACTGTGATGCTCTTAGAG+AGG 0.661195 7:+71677891 MsaT038120.1:CDS
CCCTTCCGAAGACCATCCGA+AGG 0.662669 7:-71680356 None:intergenic
GGATGCAATTGTATGACAGA+TGG 0.665267 7:+71678521 MsaT038120.1:CDS
GCAACTGTTACATCTTCGAG+CGG 0.665843 7:+71682026 MsaT038120.1:CDS
GGATGGATATTGTATGACAC+TGG 0.671850 7:+71680176 MsaT038120.1:CDS
GAAGACTCCGAATCAAGACA+CGG 0.676707 7:-71680293 None:intergenic
TTCTGTTGAGGTGATGAGGG+CGG 0.682803 7:+71677490 MsaT038120.1:CDS
GCAAGGGCCAAATATAAGCG+TGG 0.689609 7:+71678064 MsaT038120.1:CDS
AAGAGTTGGGTCTAACTCAG+TGG 0.696129 7:-71678212 None:intergenic
AAGACTCCGAATCAAGACAC+GGG 0.696680 7:-71680292 None:intergenic
TCTTCAACGTCAGCCCAAAG+CGG 0.707078 7:+71680620 MsaT038120.1:CDS
AGCATACACGAGCACATCAG+GGG 0.713965 7:+71680580 MsaT038120.1:CDS
AGGAAGAAGAGCTTCCAGTG+TGG 0.736071 7:-71676850 None:intergenic
AGATGTTAGAAAATAACCCG+GGG 0.738492 7:+71678584 MsaT038120.1:CDS
ATATCCGTACAGATATAGGG+CGG 0.752595 7:+71680780 MsaT038120.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 71676633 71682370 71676633 ID=MsaG038120
Chr7 mRNA 71676633 71682370 71676633 ID=MsaT038120.1;Parent=MsaG038120
Chr7 exon 71676633 71678647 71676633 ID=MsaT038120.1.exon1;Parent=MsaT038120.1
Chr7 CDS 71676633 71678647 71676633 ID=cds.MsaT038120.1;Parent=MsaT038120.1
Chr7 exon 71680091 71680382 71680091 ID=MsaT038120.1.exon2;Parent=MsaT038120.1
Chr7 CDS 71680091 71680382 71680091 ID=cds.MsaT038120.1;Parent=MsaT038120.1
Chr7 exon 71680495 71680674 71680495 ID=MsaT038120.1.exon3;Parent=MsaT038120.1
Chr7 CDS 71680495 71680674 71680495 ID=cds.MsaT038120.1;Parent=MsaT038120.1
Chr7 exon 71680706 71680805 71680706 ID=MsaT038120.1.exon4;Parent=MsaT038120.1
Chr7 CDS 71680706 71680805 71680706 ID=cds.MsaT038120.1;Parent=MsaT038120.1
Chr7 exon 71681953 71682131 71681953 ID=MsaT038120.1.exon5;Parent=MsaT038120.1
Chr7 CDS 71681953 71682131 71681953 ID=cds.MsaT038120.1;Parent=MsaT038120.1
Chr7 exon 71682347 71682370 71682347 ID=MsaT038120.1.exon6;Parent=MsaT038120.1
Chr7 CDS 71682347 71682370 71682347 ID=cds.MsaT038120.1;Parent=MsaT038120.1
Gene Sequence

>MsaT038120.1

ATGCAGCACAACATCCTCACCTCGATCCGAAGCACGAAAATCACGGATGGTTGTAAAAGCAATCAAATCTACGCTCTCAACACCTCCGCCGCCGCCGCCGATTCTCCGATCAACGCCGTCATCGGCGGCGGCGATTCCATCGCTGAAAAACTCTTCCATCATTTACTAGACCGCTCCAAAACTTCCGGTCGAACTAAACCGGTTGGACCCAAAACAGCCACACTGGAAGCTCTTCTTCCTTGTGGCCTTCCTTCGTCGGAGCTTCTAGAACCTTCGATTGAGCCTTGTTTGAAGCCGTTTGATTTGGTTCAAACCCTCGCCGGCGTGCGTCGCCGGATCGAGGGTGAGGGTGGGAAATTTGAAGCTTTTTTGGAACAGTGTTTGGTTTTTAAGGGGTTTTCTGATAGTAAATTGTTCCGGCGGAGTTTGCGGTCGGCGCGGCAGCATGCTGTTGATGTGCATTTGAAGATTGTGTTGGCTGCTTGGTTGAGGTATGAGAGAAGAGAAGATGAGCTTGTTGGTTCTTCTGCAATGGATTGTTGTGGAAGAAAGGTTGAATGTCCTAAGGCTAGTTTGGTTTCAGGGTGTGATCATGAATCAGGTTTTGATCATTGTTCGTGTTTGCGAAAAAATAATATTGTTAGTGTTGATAATGTTGATGGGGAATGTGAATGCTCAACTTCTTATGAAGAAGATGAGGATGGTGATTACCATGATATTTCTTTTTGTATAGGTGATAGTGAAATTAGGTGTAGTAGATATTTTATGGCGTCGCTTTCGAGGCCTTTTATGACAATGTTGTATGGTGGATTTGTTGAGTCTAGGAGGGAGAAGATAAATTTTTCTCTTAATGATTTTTCTGTTGAGGTGATGAGGGCGGTTGAGGTTTTTAGTAGAACCAAGAGGTTGAGTCAGTTTGGGAATGATCTTGTGCTTGGGATGCTTTCTTTTGCGAATAGGTTTTGTTGTACGGAGATGAAATCTGCTTGTGATGCTCATTTGGCTGCTTTGGTGTTGGATATTGATGATGCATTATTGTTGATTGAGTATGGATTGGAGGAGATGGCGTATTTGCTAGTGGCCGCTTGCTTACAGGTGTTTCTGAGAGAGCTCCCTAGCTCGATGCAACGTTTGAGTGTTATGAGATTGTTTTGTAGTGTAGAGGGTAGAGATAGATTGGCAGCGGTAGGGCATGTGTCATTTTCGTTGTATTGTTTTTTGAGTCAGGTTGCGATGGAGGAAGATATGAAATCTAACACGACTGTGATGCTCTTAGAGAGGTTAGGAGAATGTGCGATGAATGGTTGGCAGAAGCAACTTGCTTATCACCAATTAGGTGTTGTGATGTTTGAGAGAAAAGAATACAAAGATGCACAAAATTGGTTCGAGGCTGCTGTTAAGGAAGGACATATTTATTCTTCAGTGGGTGTTGCAAGGGCCAAATATAAGCGTGGTCACACATATTCATCATACAAGATGATAAACTCCCTTATTTCTGATCTTAAACCTGTTGGTTGGATGTATCAGGAAAGGTCTTTGTATTGCATCGGGAAGGAAAAAGCGATGGATTTGATCTCTGCCACTGAGTTAGACCCAACTCTTTCTTTTCCATATAAACACCGGGCTGTTTTTTTCTTGGAGGAGAATAAGATTGGAGCTGCCATTTCTGAAATCAACAAAATAATTGGTTTCAAGATTTCTCCTGACTGCCTTGAGTTGAGAGCTTGGTTCTTGATTGCCATGAAGGATTATGAAGGAGCCCTTAGGGATGTCAGGGCAATTTTGACATTGGATCCAAATTATATGATGTTCTATGGGAATATGAAAGGCGATCGCTTGGTAGAACTCCTTCGCCCTGTTGCTCAGAAGTGGAACCAGGCTGATTGTTGGATGCAATTGTATGACAGATGGTCTTCCGTTGATGATATTGGTTCTTTGGCTGTTGTTCACCAGATGTTAGAAAATAACCCGGGGAAAAGTATTTTACGCTTTCGGCAATCTTTACTTCTGTTACGGTTAAATTCTCAAAAGGCAGCCATGCGTAGTTTGCGTCTGGCCAGAAATCATTCTTCTTCAGCACATGAAAGACTTGTCTATGAAGGATGGATATTGTATGACACTGGTCATCGTGAAGAAGCAATAGCAAAGGCTGAGGAGTCGATTTCTATTCAAAGGTCATTTGAAGCTTTCTTTCTCAAAGCCTATGCATTAGCTGACCCGTGTCTTGATTCGGAGTCTTCAAAGAGTGTGATTGATCTCTTGGAGGAAGCTCTTAAATGTCCTTCGGATGGTCTTCGGAAGGGACAGGCACTCAATAATTTAGGGAGTATATATGTAGATTGCGATCAGCTAGACCTTGCTGCTGACTGCTACAAGCATGCACTTAACATCAAGCATACACGAGCACATCAGGGGTTGGCACGTGTATATCATCTTCAACGTCAGCCCAAAGCGGCATATGATGAGATGACAAAGCTGATAGAAAAGCGTTCAGAATATTGCGATCGTGACCCTGCAAAGAACGATCTTAGTTTGGCAACACAGTTGGATCCTCTAAGGACATATCCGTACAGATATAGGGCGGCAGTGTTAATGGACGATCATAAGGAAACTGAAGCAATTGCAGAGCTTTCAAGGGCCATCAACTTTAAGCCAGAATTGCAACTGTTACATCTTCGAGCGGCATTTTATGAATCAATGGATGATTACGTTTCTACTGTCCAAGACTGTGAAGCGGCCCTTTGTCTTGATCCTAGCCATGCTGAGGAGGGAAAAGGTGAGTTAGAATGA
Protein sequence

>MsaT038120.1

MQHNILTSIRSTKITDGCKSNQIYALNTSAAAADSPINAVIGGGDSIAEKLFHHLLDRSKTSGRTKPVGPKTATLEALLPCGLPSSELLEPSIEPCLKPFDLVQTLAGVRRRIEGEGGKFEAFLEQCLVFKGFSDSKLFRRSLRSARQHAVDVHLKIVLAAWLRYERREDELVGSSAMDCCGRKVECPKASLVSGCDHESGFDHCSCLRKNNIVSVDNVDGECECSTSYEEDEDGDYHDISFCIGDSEIRCSRYFMASLSRPFMTMLYGGFVESRREKINFSLNDFSVEVMRAVEVFSRTKRLSQFGNDLVLGMLSFANRFCCTEMKSACDAHLAALVLDIDDALLLIEYGLEEMAYLLVAACLQVFLRELPSSMQRLSVMRLFCSVEGRDRLAAVGHVSFSLYCFLSQVAMEEDMKSNTTVMLLERLGECAMNGWQKQLAYHQLGVVMFERKEYKDAQNWFEAAVKEGHIYSSVGVARAKYKRGHTYSSYKMINSLISDLKPVGWMYQERSLYCIGKEKAMDLISATELDPTLSFPYKHRAVFFLEENKIGAAISEINKIIGFKISPDCLELRAWFLIAMKDYEGALRDVRAILTLDPNYMMFYGNMKGDRLVELLRPVAQKWNQADCWMQLYDRWSSVDDIGSLAVVHQMLENNPGKSILRFRQSLLLLRLNSQKAAMRSLRLARNHSSSAHERLVYEGWILYDTGHREEAIAKAEESISIQRSFEAFFLKAYALADPCLDSESSKSVIDLLEEALKCPSDGLRKGQALNNLGSIYVDCDQLDLAADCYKHALNIKHTRAHQGLARVYHLQRQPKAAYDEMTKLIEKRSEYCDRDPAKNDLSLATQLDPLRTYPYRYRAAVLMDDHKETEAIAELSRAINFKPELQLLHLRAAFYESMDDYVSTVQDCEAALCLDPSHAEEGKGELE*