Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038130 | XP_003623338.1 | 90.452 | 199 | 19 | 0 | 1 | 199 | 209 | 407 | 9.52e-122 | 367 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038130 | tr|G7KSD5|G7KSD5_MEDTR | 90.452 | 199 | 19 | 0 | 1 | 199 | 209 | 407 | 4.55e-122 | 367 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG038130 | TF | GRAS |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG021585 | MsaG038130 | 0.899511 | 4.231551e-77 | 2.103133e-73 |
| MsaG038130 | MsaG039816 | 0.839674 | 2.539624e-57 | 9.812996e-55 |
| MsaG038130 | MsaG031556 | 0.808080 | 6.281937e-50 | 1.024282e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG038130 | MtrunA17_Chr7g0243151 | 90.452 | 199 | 19 | 0 | 1 | 199 | 209 | 407 | 8.76e-126 | 367 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTGACATAATTGATTAT+TGG | 0.190898 | 7:+71766227 | MsaT038130.1:CDS |
| TGAAATTGACATCAATTAAA+TGG | 0.209268 | 7:+71766311 | MsaT038130.1:CDS |
| GACATAATTGATTATTGGTT+TGG | 0.287363 | 7:+71766232 | MsaT038130.1:CDS |
| ATACTCATAAAGTTCCCTTT+TGG | 0.352223 | 7:-71766374 | None:intergenic |
| ATGAAAATTCTGAATGTGTT+TGG | 0.359147 | 7:+71766338 | MsaT038130.1:CDS |
| TCTTGTATGCCTCGCCTTTG+AGG | 0.374060 | 7:-71766093 | None:intergenic |
| AGTGAGTCTTCCACACTTGA+TGG | 0.390562 | 7:+71765919 | MsaT038130.1:CDS |
| GAAATTGAAGGTAGAGGAAA+AGG | 0.406350 | 7:+71766414 | MsaT038130.1:CDS |
| GAAGGTAGAGGAAAAGGAAT+TGG | 0.435590 | 7:+71766420 | MsaT038130.1:CDS |
| ATTGATTATTGGTTTGGAAA+TGG | 0.443553 | 7:+71766238 | MsaT038130.1:CDS |
| GTCTCAAGAGAGTCACCAAT+TGG | 0.467270 | 7:-71766025 | None:intergenic |
| AGATCAAAATAATGAGCAAA+CGG | 0.470223 | 7:+71766458 | MsaT038130.1:CDS |
| GAAACAGCCTCCAAGAGAAA+AGG | 0.477495 | 7:-71766271 | None:intergenic |
| GCTCAAGAAGTGACAAATCA+TGG | 0.497130 | 7:+71766064 | MsaT038130.1:CDS |
| ATCATGTGGTTTGAAATTGA+AGG | 0.512511 | 7:+71766402 | MsaT038130.1:CDS |
| CATGGTGAAGCCTTGGAGAA+AGG | 0.516575 | 7:+71765947 | MsaT038130.1:CDS |
| GGAAGCACATGGTGAAGCCT+TGG | 0.523810 | 7:+71765940 | MsaT038130.1:CDS |
| TGGTTTGAAATTGAAGGTAG+AGG | 0.525487 | 7:+71766408 | MsaT038130.1:CDS |
| GGTTTGGAAATGGTGTTCAA+TGG | 0.527955 | 7:+71766248 | MsaT038130.1:CDS |
| GATCAAAATAATGAGCAAAC+GGG | 0.531290 | 7:+71766459 | MsaT038130.1:CDS |
| TATGAGTATGCTAAATCATG+TGG | 0.552563 | 7:+71766388 | MsaT038130.1:CDS |
| TCAAGTGTGGAAGACTCACT+TGG | 0.579458 | 7:-71765916 | None:intergenic |
| CAAAATAATGAGCAAACGGG+TGG | 0.609780 | 7:+71766462 | MsaT038130.1:CDS |
| TTCATCACAATCTTCTAACA+TGG | 0.616759 | 7:-71766206 | None:intergenic |
| CCATGTGCTTCCATCAAGTG+TGG | 0.618160 | 7:-71765929 | None:intergenic |
| CCACACTTGATGGAAGCACA+TGG | 0.654957 | 7:+71765929 | MsaT038130.1:CDS |
| AATCATGGAGAATACCTCAA+AGG | 0.661739 | 7:+71766079 | MsaT038130.1:CDS |
| AAGAGAGTCACCAATTGGAG+TGG | 0.663623 | 7:-71766020 | None:intergenic |
| TGGAGAATACCTCAAAGGCG+AGG | 0.686092 | 7:+71766084 | MsaT038130.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 71765902 | 71766513 | 71765902 | ID=MsaG038130 |
| Chr7 | mRNA | 71765902 | 71766513 | 71765902 | ID=MsaT038130.1;Parent=MsaG038130 |
| Chr7 | exon | 71765902 | 71766513 | 71765902 | ID=MsaT038130.1.exon1;Parent=MsaT038130.1 |
| Chr7 | CDS | 71765902 | 71766513 | 71765902 | ID=cds.MsaT038130.1;Parent=MsaT038130.1 |
| Gene Sequence |
| Protein sequence |
