AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG038266

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038266 MtrunA17_Chr7g0244571 92.711 343 15 1 1 333 1 343 0.0 604
MsaG038266 MtrunA17_Chr4g0013081 47.643 403 114 15 1 333 1 376 2.18e-85 265
MsaG038266 MtrunA17_Chr5g0432531 47.087 206 92 6 128 333 60 248 2.07e-46 160
MsaG038266 MtrunA17_Chr2g0284471 34.839 155 94 2 185 333 156 309 5.02e-22 95.9
MsaG038266 MtrunA17_Chr8g0379681 50.000 88 38 2 271 358 258 339 9.41e-21 92.4
MsaG038266 MtrunA17_Chr4g0025901 46.479 71 38 0 262 332 90 160 5.90e-15 72.8
MsaG038266 MtrunA17_Chr8g0390531 52.459 61 29 0 271 331 258 318 1.84e-14 73.9
MsaG038266 MtrunA17_Chr4g0075391 50.000 60 30 0 273 332 83 142 3.86e-14 69.7
MsaG038266 MtrunA17_Chr1g0177881 44.118 68 38 0 264 331 95 162 9.41e-13 66.6
MsaG038266 MtrunA17_Chr5g0435131 36.264 91 50 1 250 332 62 152 1.78e-12 65.1
MsaG038266 MtrunA17_Chr4g0025911 45.312 64 35 0 269 332 91 154 6.00e-12 63.9
MsaG038266 MtrunA17_Chr3g0141471 29.798 198 103 4 152 335 90 265 1.69e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG038266 AT1G06920.1 33.795 361 151 15 1 331 1 303 9.61e-37 136
MsaG038266 AT2G30400.1 32.329 365 160 11 1 331 1 312 3.59e-35 132
MsaG038266 AT5G58360.1 30.548 347 154 11 1 329 5 282 1.65e-30 119
MsaG038266 AT5G01840.1 46.923 130 50 4 214 340 151 264 1.12e-21 94.4
MsaG038266 AT4G18830.1 40.000 105 55 2 235 331 238 342 1.30e-18 87.0
MsaG038266 AT5G19650.1 51.613 62 30 0 270 331 153 214 5.77e-15 73.9
MsaG038266 AT3G52525.1 45.000 60 33 0 272 331 67 126 2.61e-11 62.0

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACGGTCTGTTCTGATTTC+TGG 0.132362 7:-74309298 None:intergenic
CTTTGTTGTCTATAATATTT+TGG 0.178812 7:-74316680 None:intergenic
ACAAATATCGAGCTGGTATT+TGG 0.212439 7:-74316857 None:intergenic
TCAAAGTGTTCAAGCAAATA+TGG 0.233458 7:+74309908 MsaT038266.1:CDS
AATGATGAACACAACGTTAA+AGG 0.235673 7:+74309523 MsaT038266.1:CDS
TTCTTCCTTTGTTGTGTTGT+TGG 0.243928 7:-74309025 None:intergenic
CATTAGAACATCAAAGGATT+TGG 0.325611 7:+74309828 MsaT038266.1:CDS
TGAAGATTCTATTGCTTGTT+TGG 0.331737 7:-74309147 None:intergenic
TTTGTTGTGTTGTTGGTGTT+TGG 0.367246 7:-74309018 None:intergenic
GAGAGATAATGGGAAATTAC+AGG 0.369625 7:+74308927 None:intergenic
ATGATGAACACAACGTTAAA+GGG 0.389606 7:+74309524 MsaT038266.1:CDS
TACGTCTTCCACCATCGTTT+CGG 0.400519 7:+74309699 MsaT038266.1:CDS
TCGAGCTGGTATTTGGTTTG+TGG 0.418678 7:-74316850 None:intergenic
CCATCGTTTCGGCTGGCAAC+CGG 0.420330 7:+74309710 MsaT038266.1:CDS
TTGGATGAATGATACCACTA+TGG 0.424093 7:+74316719 MsaT038266.1:CDS
GTTCACCAGGTGTGAAACTT+AGG 0.443025 7:+74309623 MsaT038266.1:CDS
GTAGAGGGTGTCAAGGGAGG+AGG 0.443902 7:+74316786 MsaT038266.1:CDS
TCTGTTCTGATTTCTGGCTC+AGG 0.447190 7:-74309292 None:intergenic
TGGCAACCGGAGAAGTCTTT+CGG 0.457447 7:+74309723 MsaT038266.1:CDS
TCGGGTTTGGTTACAATTGG+TGG 0.459629 7:-74309433 None:intergenic
GGAGATGATGATGAAGTCTT+TGG 0.474574 7:-74309226 None:intergenic
CAGACATGATGCCTAATGCT+TGG 0.484513 7:+74308960 MsaT038266.1:CDS
CTGTTCTGATTTCTGGCTCA+GGG 0.485970 7:-74309291 None:intergenic
AGCTTGTAAAACCAAGCATT+AGG 0.493200 7:-74308971 None:intergenic
TCGATAATGATATCGTTTGT+CGG 0.505634 7:-74309328 None:intergenic
AGATTCTCTGTTAGTTCACC+AGG 0.512695 7:+74309610 MsaT038266.1:CDS
ATTCACCAAGAATTTCAAAC+CGG 0.519789 7:+74309650 MsaT038266.1:CDS
TCTTCCACCATCGTTTCGGC+TGG 0.529072 7:+74309703 MsaT038266.1:CDS
CTATCAAGTGGAGAATCAAA+TGG 0.529685 7:-74309247 None:intergenic
ACTCATCTGAATTCAAAGAA+AGG 0.530280 7:-74309870 None:intergenic
CTAACAGAGAATCTTCTACT+TGG 0.532521 7:-74309601 None:intergenic
TTCTTCGACACCACCCATAG+TGG 0.538498 7:-74316733 None:intergenic
GAACAACATTAGAACATCAA+AGG 0.551741 7:+74309822 MsaT038266.1:CDS
GGAAAATTCAAACACATGGC+CGG 0.552191 7:+74309671 MsaT038266.1:CDS
TATTAACACAAATATCGAGC+TGG 0.557706 7:-74316864 None:intergenic
AACTATCCGAAAGACTTCTC+CGG 0.559644 7:-74309729 None:intergenic
TTTCTTGGATTGCTTTGGTG+GGG 0.559822 7:-74309097 None:intergenic
ATGGGTGGTGTCGAAGAAGA+AGG 0.562258 7:+74316738 MsaT038266.1:CDS
AAACACCAACAACACAACAA+AGG 0.563217 7:+74309020 MsaT038266.1:CDS
TGGGTAGAGGGTGTCAAGGG+AGG 0.581976 7:+74316783 MsaT038266.1:CDS
GTTGCCAGCCGAAACGATGG+TGG 0.586748 7:-74309707 None:intergenic
CCGGTTGCCAGCCGAAACGA+TGG 0.591822 7:-74309710 None:intergenic
GATTCTCTAAAATCTCCTTG+TGG 0.610956 7:-74309778 None:intergenic
AACCGGAAAATTCAAACACA+TGG 0.611341 7:+74309667 MsaT038266.1:CDS
GATTCTGCAGAGCTATCAAG+TGG 0.613063 7:-74309259 None:intergenic
TAATCATTCTAAGTTTCATG+TGG 0.614253 7:+74316637 MsaT038266.1:intron
TTGATCCTAAGTTTCACACC+TGG 0.631177 7:-74309628 None:intergenic
TGGATGAATGATACCACTAT+GGG 0.659875 7:+74316720 MsaT038266.1:CDS
AAGTCATCATTGAATCCACA+AGG 0.674680 7:+74309763 MsaT038266.1:CDS
ATGAATGATACCACTATGGG+TGG 0.746171 7:+74316723 MsaT038266.1:CDS
CGTTTGTCGGAAGAAGAACA+CGG 0.768531 7:-74309315 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr7 gene 74308935 74316884 74308935 ID=MsaG038266
Chr7 mRNA 74308935 74316884 74308935 ID=MsaT038266.1;Parent=MsaG038266
Chr7 exon 74308935 74309929 74308935 ID=MsaT038266.1.exon1;Parent=MsaT038266.1
Chr7 CDS 74308935 74309929 74308935 ID=cds.MsaT038266.1;Parent=MsaT038266.1
Chr7 exon 74316650 74316884 74316650 ID=MsaT038266.1.exon2;Parent=MsaT038266.1
Chr7 CDS 74316650 74316884 74316650 ID=cds.MsaT038266.1;Parent=MsaT038266.1
Gene Sequence

>MsaT038266.1

ATGGGAAATTACAGGTTCAAATTATCAGACATGATGCCTAATGCTTGGTTTTACAAGCTTAGAGACATGGGAAGAACAAGAAACCAAACACCAACAACACAACAAAGGAAGAAAAAGCAATCTTCAAGTTCAACAACCTCAAACACATCAAAAACAAAGCAACCCCACCAAAGCAATCCAAGAAAATCTTACTACTTTACAAGAGAGCTTAACCAAACAAGCAATAGAATCTTCAACACTTCACCACCAAGAAAATCATCATCAAAACAACAAAGACCAAAAAGAAGAACACCAAAGACTTCATCATCATCTCCATTTGATTCTCCACTTGATAGCTCTGCAGAATCAGAAGAACTCCCTGAGCCAGAAATCAGAACAGACCGTGTTCTTCTTCCGACAAACGATATCATTATCGATGTTGACGACAATTCTATCATAGCAACAAGAAAAGATGATAAGCTAAATGAGTATGATTCATTATCAGAACTCATGAAACTTCCACCAATTGTAACCAAACCCGAAAAGTTCAACTACTTGTTAAACTATGCAAAGAAGAAAGAAACAAAACCCAGAAGCAAAAATTCATCAAATGATGAACACAACGTTAAAGGGTCACTAAAAGTCAAGATTTTGAAGGAAGAACAGAGGAGCATCAACACAAACACACCAAGTAGAAGATTCTCTGTTAGTTCACCAGGTGTGAAACTTAGGATCAATTCACCAAGAATTTCAAACCGGAAAATTCAAACACATGGCCGGAAAAGTACGTCTTCCACCATCGTTTCGGCTGGCAACCGGAGAAGTCTTTCGGATAGTTTTGCAATTGTGAAGTCATCATTGAATCCACAAGGAGATTTTAGAGAATCAATGGTGGAGATGATTGTTCAGAACAACATTAGAACATCAAAGGATTTGGAAGATCTTCTTGCTTGTTACCTTTCTTTGAATTCAGATGAGTATCATGAACTTATTATCAAAGTGTTCAAGCAAATATGTTTCATGTGGAAGTGCACAAACTTGAAGCACCAAAATATTATAGACAACAAAGAAATTAAGCAAAAAACTTGGATGAATGATACCACTATGGGTGGTGTCGAAGAAGAAGGATATGCAGCTTTTAAATACTTTTGGGTAGAGGGTGTCAAGGGAGGAGGAAACAAATGCGATCGAAACACATTTGTTGCACAATATCAATCCACAAACCAAATACCAGCTCGATATTTGTGTTAA
Protein sequence

>MsaT038266.1

MGNYRFKLSDMMPNAWFYKLRDMGRTRNQTPTTQQRKKKQSSSSTTSNTSKTKQPHQSNPRKSYYFTRELNQTSNRIFNTSPPRKSSSKQQRPKRRTPKTSSSSPFDSPLDSSAESEELPEPEIRTDRVLLPTNDIIIDVDDNSIIATRKDDKLNEYDSLSELMKLPPIVTKPEKFNYLLNYAKKKETKPRSKNSSNDEHNVKGSLKVKILKEEQRSINTNTPSRRFSVSSPGVKLRINSPRISNRKIQTHGRKSTSSTIVSAGNRRSLSDSFAIVKSSLNPQGDFRESMVEMIVQNNIRTSKDLEDLLACYLSLNSDEYHELIIKVFKQICFMWKCTNLKHQNIIDNKEIKQKTWMNDTTMGGVEEEGYAAFKYFWVEGVKGGGNKCDRNTFVAQYQSTNQIPARYLC*